pycom05g09640

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012239
Physical Location & Seq
Reverse (-)
12891501 .. 12894485
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g09640.4

Sequence Viewer

Length: 1098 bp
ATGACAGCTGTCCCTCCGCCGGCGCCAGGCCGAGAGCTCTCCAACCCCCCCACGGACGGCATCTCCAACCTCCGCTTCTCCAACCATAGCGACCACCTGCTCGTCTCCTCTTGGGACAAGGTACGCCCTCGCCTTCCTAACTCTCGCTGCGCGTCCTGCTCCCCCATCTGTTTCCAACCCCTCTCTCTCTCATTGCGCAGACCGTCCGATTGTACGACGCCAGCGCCAATGTGCTGCGAGGAGAGTTCATGCACGGAGGTCCCGTTCTCGATTGCTGCTTCCACGATGATTCCTCCGGGTTCAGCGCCGGTGCCGACAACACTGTTCGTATTCAGCTCCAGTAAGGAAGACATTTTAGGAAGGCACGACGCGCCGGTGCGTTGCATTGAATACTCTTATGCAGCAGGGCAGTTGATTACTGGTAGTTGGGATAAAACAGTTAAGTGTTGGGACCCTAGAGGCGCAAGTGGACAGGAGAGAACTCTTGTTGGGTCATACCCACAACCCGAGCGTGTTTATTCTCTTTCACTTGTGGGGAACCGATTAGTGGTTGCAACCGCCGGAAGACATGTCAATATCTATGATTTGAGAAATATGTCCCAGCCTGAGCAACGGAGGGAGTCTTCCTTAAAGTATCAAACTCGATGTGTGCGCTGTTATCCCAATGGAACAGGTTATGCTCTCAGTTCTGTTGAAGGACGGGTTGCAATGGAGTTTTTTGATCTCTCCGAGGCTAGTCAAACTAAAAAATATGCATTTAAGTGTCATAGAAAATCAGAGGCTGGAAGGGACATTGTGTATCCAGTAAATGCAATTGCATTCCACCCCGTCTATGGTACTTTTGCTACTGGAGGTTGTGATGGTTATGTGAACGTATGGGACGGGAACAACAAGAAGAGGCTCTATCAGTACTCGAAGTACCCAACAAGCATTGCGGCACTTTCTTTTAGCAGAGATGGCCGCCTTTTGGCTGTGGCATCAAGTTACACATATGAAGAGGGAGATAAACCCCATGAGCCAGATGCCATCTTTGTTCGAAGTGTAAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTGTACCCTAATCCGCCAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

366

Amino Acids

40.3

Weight (kDa)

8.71

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 105
Acc16I TGCGCA 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 105
AccB1I GGYRCC 2 cut(s) 22, 310
AccII CGCG 2 cut(s) 152, 371
AciI CCGC 6 cut(s) 17, 73, 558, 935, 961, 1088
AcoI YGGCCR 1 cut(s) 958
AcyI GRCGYC 2 cut(s) 23, 218
AfaI GTAC 6 cut(s) 123, 214, 838, 911, 920, 1079
AfiI CCNNNNNNNGG 7 cut(s) 19, 26, 52, 56, 547, 833, 967
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 2 cut(s) 389, 695
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 3 cut(s) 8, 37, 336
AluI AGCT 3 cut(s) 8, 37, 336
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 782
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 2 cut(s) 28, 958
ApeKI GCWGC 4 cut(s) 147, 234, 275, 401
AspLEI GCGC 8 cut(s) 25, 152, 198, 226, 307, 373, 464, 654
AspS9I GGNCC 2 cut(s) 259, 451
AsuC2I CCSGG 1 cut(s) 297
AsuII TTCGAA 1 cut(s) 1036
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 2 cut(s) 259, 451
BanI GGYRCC 2 cut(s) 22, 310
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 3 cut(s) 354, 571, 615
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 4 cut(s) 134, 221, 262, 413
BccI CCATC 4 cut(s) 173, 854, 950, 1034
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 27
BciVI GTATCC 1 cut(s) 810
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 2 cut(s) 456, 735
BfoI RGCGCY 3 cut(s) 26, 227, 308
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 810
BisI GCNGC 6 cut(s) 148, 235, 276, 402, 936, 961
BlsI GCNGC 6 cut(s) 149, 236, 277, 403, 937, 962
BmcAI AGTACT 1 cut(s) 911
Bme1390I CCNGG 2 cut(s) 27, 297
Bme18I GGWCC 2 cut(s) 259, 451
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 259, 451
BmiI GGNNCC 6 cut(s) 24, 261, 312, 452, 453, 539
BmrFI CCNGG 2 cut(s) 27, 297
BmsI GCATC 3 cut(s) 69, 986, 1012
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 3 cut(s) 354, 571, 615
BpmI CTGGAG 2 cut(s) 322, 870
Bpu10I CCTNAGC 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 297
BsaHI GRCGYC 2 cut(s) 23, 218
BsaJI CCNNGG 2 cut(s) 51, 729
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 248, 278
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 26, 52, 56, 547, 833, 967
Bse118I RCCGGY 3 cut(s) 19, 307, 373
Bse1I ACTGG 4 cut(s) 339, 424, 803, 853
Bse3DI GCAATG 3 cut(s) 191, 714, 930
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 51, 729
BseLI CCNNNNNNNGG 7 cut(s) 19, 26, 52, 56, 547, 833, 967
BseMI GCAATG 3 cut(s) 191, 714, 930
BseMII CTCAG 2 cut(s) 597, 697
BseNI ACTGG 4 cut(s) 339, 424, 803, 853
BseRI GAGGAG 2 cut(s) 97, 254
BseXI GCAGC 4 cut(s) 134, 221, 262, 413
BseYI CCCAGC 1 cut(s) 600
Bsh1236I CGCG 2 cut(s) 152, 371
BshFI GGCC 2 cut(s) 30, 960
BshNI GGYRCC 2 cut(s) 22, 310
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 5 cut(s) 20, 296, 308, 374, 561
BslFI GGGAC 6 cut(s) 128, 245, 464, 583, 803, 893
BslI CCNNNNNNNGG 7 cut(s) 19, 26, 52, 56, 547, 833, 967
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 6 cut(s) 128, 245, 464, 583, 803, 893
BsmI GAATGC 1 cut(s) 818
BsnI GGCC 2 cut(s) 30, 960
BsoBI CYCGRG 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 1036
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 1 cut(s) 721
BspACI CCGC 6 cut(s) 17, 73, 558, 935, 961, 1088
BspANI GGCC 2 cut(s) 30, 960
BspCNI CTCAG 2 cut(s) 598, 696
BspFNI CGCG 2 cut(s) 152, 371
BspLI GGNNCC 6 cut(s) 24, 261, 312, 452, 453, 539
BspMI ACCTGC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 1036
BspT107I GGYRCC 2 cut(s) 22, 310
BsrDI GCAATG 3 cut(s) 191, 714, 930
BsrFI RCCGGY 3 cut(s) 19, 307, 373
BsrI ACTGG 4 cut(s) 339, 424, 803, 853
BssAI RCCGGY 3 cut(s) 19, 307, 373
BssECI CCNNGG 2 cut(s) 51, 729
BssMI GATC 1 cut(s) 721
BssNI GRCGYC 2 cut(s) 23, 218
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 204, 324, 439
Bst6I CTCTTC 2 cut(s) 890, 990
BstACI GRCGYC 2 cut(s) 23, 218
BstBI TTCGAA 1 cut(s) 1036
BstC8I GCNNGC 3 cut(s) 21, 222, 1092
BstDEI CTNAG 2 cut(s) 606, 683
BstDSI CCRYGG 1 cut(s) 51
BstFNI CGCG 2 cut(s) 152, 371
BstH2I RGCGCY 3 cut(s) 26, 227, 308
BstHHI GCGC 8 cut(s) 25, 152, 198, 226, 307, 373, 464, 654
BstKTI GATC 1 cut(s) 724
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 1 cut(s) 721
BstMWI GCNNNNNNNGC 3 cut(s) 156, 370, 957
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 572
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 25, 295
BstUI CGCG 2 cut(s) 152, 371
BstV1I GCAGC 4 cut(s) 134, 221, 262, 413
BstV2I GAAGAC 3 cut(s) 354, 571, 615
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 2 cut(s) 30, 960
BtgI CCRYGG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 320
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 21, 222, 1092
CaiI CAGNNNCTG 1 cut(s) 782
CfoI GCGC 8 cut(s) 25, 152, 198, 226, 307, 373, 464, 654
Cfr10I RCCGGY 3 cut(s) 19, 307, 373
Cfr13I GGNCC 2 cut(s) 259, 451
CseI GACGC 3 cut(s) 141, 226, 377
Csp6I GTAC 6 cut(s) 122, 213, 837, 910, 919, 1078
CviAII CATG 3 cut(s) 249, 569, 1013
CviQI GTAC 6 cut(s) 122, 213, 837, 910, 919, 1078
DdeI CTNAG 2 cut(s) 606, 683
DinI GGCGCC 1 cut(s) 24
DpnI GATC 1 cut(s) 723
DpnII GATC 1 cut(s) 721
EaeI YGGCCR 1 cut(s) 958
Eam1104I CTCTTC 2 cut(s) 890, 990
EarI CTCTTC 2 cut(s) 890, 990
EciI GGCGGA 2 cut(s) 6, 1077
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 259, 451
Eco53kI GAGCTC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 2 cut(s) 259, 451
EcoRII CCWGG 1 cut(s) 25
EcoT22I ATGCAT 1 cut(s) 757
EcoT38I GRGCYC 1 cut(s) 39
EgeI GGCGCC 1 cut(s) 24
EheI GGCGCC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 3 cut(s) 252, 572, 1016
FaqI GGGAC 6 cut(s) 128, 245, 464, 583, 803, 893
FatI CATG 3 cut(s) 248, 568, 1012
FauNDI CATATG 1 cut(s) 991
Fnu4HI GCNGC 6 cut(s) 148, 235, 276, 402, 936, 961
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 6 cut(s) 148, 235, 276, 402, 936, 961
FspBI CTAG 2 cut(s) 456, 735
FspI TGCGCA 1 cut(s) 197
GlaI GCGC 8 cut(s) 24, 151, 197, 225, 306, 372, 463, 653
GluI GCNGC 6 cut(s) 148, 235, 276, 402, 936, 961
GsaI CCCAGC 1 cut(s) 604
GsuI CTGGAG 2 cut(s) 322, 870
HaeII RGCGCY 3 cut(s) 26, 227, 308
HaeIII GGCC 2 cut(s) 30, 960
HapII CCGG 5 cut(s) 20, 296, 308, 374, 561
HgaI GACGC 3 cut(s) 141, 226, 377
HhaI GCGC 8 cut(s) 25, 152, 198, 226, 307, 373, 464, 654
Hin1I GRCGYC 2 cut(s) 23, 218
Hin1II CATG 3 cut(s) 252, 572, 1016
Hin6I GCGC 8 cut(s) 23, 150, 196, 224, 305, 371, 462, 652
HinP1I GCGC 8 cut(s) 23, 150, 196, 224, 305, 371, 462, 652
HinfI GANTC 2 cut(s) 289, 620
HpaII CCGG 5 cut(s) 20, 296, 308, 374, 561
Hpy166II GTNNAC 3 cut(s) 470, 871, 1078
Hpy188I TCNGA 3 cut(s) 208, 730, 778
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 3 cut(s) 470, 871, 1078
Hpy99I CGWCG 2 cut(s) 220, 371
HpyAV CCTTC 4 cut(s) 143, 354, 689, 780
HpyCH4III ACNGT 3 cut(s) 204, 324, 439
HpyCH4IV ACGT 1 cut(s) 873
HpyCH4V TGCA 8 cut(s) 252, 384, 401, 554, 707, 755, 812, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 370, 957
HpyF3I CTNAG 2 cut(s) 606, 683
HpySE526I ACGT 1 cut(s) 873
Hsp92I GRCGYC 2 cut(s) 23, 218
Hsp92II CATG 3 cut(s) 252, 572, 1016
HspAI GCGC 8 cut(s) 23, 150, 196, 224, 305, 371, 462, 652
KasI GGCGCC 1 cut(s) 22
KflI GGGWCCC 1 cut(s) 451
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 721
LmnI GCTCC 2 cut(s) 164, 341
Lsp1109I GCAGC 4 cut(s) 134, 221, 262, 413
LweI GCATC 3 cut(s) 69, 986, 1012
MaeI CTAG 2 cut(s) 456, 735
MaeII ACGT 1 cut(s) 873
MaeIII GTNAC 1 cut(s) 983
MalI GATC 1 cut(s) 723
MboI GATC 1 cut(s) 721
MboII GAAGA 5 cut(s) 359, 576, 615, 907, 1007
MfeI CAATTG 1 cut(s) 813
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 813
Mly113I GGCGCC 1 cut(s) 23
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 4 cut(s) 66, 90, 105, 199
Mph1103I ATGCAT 1 cut(s) 757
MreI CGCCGGCG 1 cut(s) 19
MroNI GCCGGC 1 cut(s) 19
MseI TTAA 3 cut(s) 441, 629, 759
MslI CAYNNNNRTG 1 cut(s) 760
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 5 cut(s) 20, 296, 308, 374, 561
MspR9I CCNGG 2 cut(s) 27, 297
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 818
MvaI CCWGG 1 cut(s) 27
MvnI CGCG 2 cut(s) 152, 371
MwoI GCNNNNNNNGC 3 cut(s) 156, 370, 957
NaeI GCCGGC 1 cut(s) 21
NarI GGCGCC 1 cut(s) 23
NciI CCSGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 991
NdeII GATC 1 cut(s) 721
NgoMIV GCCGGC 1 cut(s) 19
NlaIII CATG 3 cut(s) 252, 572, 1016
NlaIV GGNNCC 6 cut(s) 24, 261, 312, 452, 453, 539
NmeAIII GCCGAG 1 cut(s) 56
NsbI TGCGCA 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 757
NspI RCATGY 1 cut(s) 572
NspV TTCGAA 1 cut(s) 1036
PaqCI CACCTGC 1 cut(s) 105
PciI ACATGT 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 260
PctI GAATGC 1 cut(s) 818
PdiI GCCGGC 1 cut(s) 21
PfeI GAWTC 1 cut(s) 289
PkrI GCNGC 6 cut(s) 149, 236, 277, 403, 937, 962
PleI GAGTC 1 cut(s) 628
PluTI GGCGCC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 628
PpuMI RGGWCCY 2 cut(s) 259, 451
PscI ACATGT 1 cut(s) 568
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 2 cut(s) 259, 451
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 600
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 6 cut(s) 24, 261, 312, 452, 453, 539
PspPI GGNCC 2 cut(s) 259, 451
PspPPI RGGWCCY 2 cut(s) 259, 451
PstNI CAGNNNCTG 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 6 cut(s) 123, 214, 838, 911, 920, 1079
RsaNI GTAC 6 cut(s) 122, 213, 837, 910, 919, 1078
RseI CAYNNNNRTG 1 cut(s) 760
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 441, 629, 759
SatI GCNGC 6 cut(s) 148, 235, 276, 402, 936, 961
Sau3AI GATC 1 cut(s) 721
Sau96I GGNCC 2 cut(s) 259, 451
ScaI AGTACT 1 cut(s) 911
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 2 cut(s) 27, 297
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 3 cut(s) 69, 986, 1012
SfoI GGCGCC 1 cut(s) 24
SfuI TTCGAA 1 cut(s) 1036
SgrAI CRCCGGYG 3 cut(s) 19, 307, 373
SinI GGWCC 2 cut(s) 259, 451
SmiMI CAYNNNNRTG 1 cut(s) 760
Sse9I AATT 1 cut(s) 813
SsiI CCGC 6 cut(s) 17, 73, 558, 935, 961, 1088
SspDI GGCGCC 1 cut(s) 22
SspMI CTAG 2 cut(s) 456, 735
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 25, 295
TaaI ACNGT 3 cut(s) 204, 324, 439
TaiI ACGT 1 cut(s) 876
TaqI TCGA 4 cut(s) 269, 643, 914, 1036
TasI AATT 1 cut(s) 813
TatI WGTACW 1 cut(s) 909
TauI GCSGC 2 cut(s) 938, 963
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 3 cut(s) 441, 629, 759
Tru9I TTAA 3 cut(s) 441, 629, 759
TscAI CASTG 1 cut(s) 327
TseI GCWGC 4 cut(s) 147, 234, 275, 401
TspDTI ATGAA 2 cut(s) 237, 1008
TspGWI ACGGA 3 cut(s) 68, 269, 628
TspRI CASTG 1 cut(s) 327
VpaK11BI GGWCC 2 cut(s) 259, 451
XceI RCATGY 1 cut(s) 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 830
XspI CTAG 2 cut(s) 456, 735
ZrmI AGTACT 1 cut(s) 911
Zsp2I ATGCAT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.