Prupe.8G143300_v2.0.a1

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0012239
Physical Location & Seq
Reverse (-)
16021614 .. 16025550
3937 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G143300.1

Sequence Viewer

Length: 1026 bp
ATGACAGCTGTCCCTCCGCCGGCGCCGGGCCGCGAGCTCTCCAGCCCCCCTTCCGACGGCATCTCTAACCTCCGCTTCTCCAACCACAGCGATCATTTGCTCGTCTCTTCATGGGACAAGACCGTCCGATTGTACGACGCCAGCGCCAATGTGCTGCGAGGAGAGTTCAAGCACGGCGGTCCCGTACTCGATTGCTGCTTCCACGACGACTCTTCCGGCTTCAGCGCCGGCGCCGACAACACTGTTAGACGGCTCGTTTTCAGCTCTAGTAAGGAAGACATCCTAGGAAGGCACGATGCACCAGTGCGGTGCGTTGAGTACTCTTATGCAGCAGGGCAGTTGATCACTGGTAGTTGGGATAAGACACTCAAGTGTTGGGACCCCAGAGGTGCAAGCGGACAGGAGCGTACTCTTGTTGGGACGTACCCACAACCCGAACGTGTTTATTCTCTTTCTCTTGTTGGAAATCGGTTGGTGGTTGCAACAGCAGGAAGACACGTCAATGTCTATGATTTGAGAAATATGTCCCAGCCTGAGCAACGGAGGGAATCTTCATTGAAGTATCAAACTCGATGTGTGCGCTGTTATCCTAATGGAACAGGTTATGCTCTTAGTTCTGTTGAAGGACGGGTTGCGATGGAGTTTTTTGATCTCTCAGAGGCTAGTCAGACCAAAAAATATGCATTCAAGTGTCACAGAAAATCAGAGGCTGGAAGGGACATTGTCTATCCAGTAAATGCAATTGCATTCCACCCCATCTATGGCACTTTTGCTACTGGAGGTTGTGATGGCTATGTGAATGTATGGGATGGGAACAACAAGAAGAGGCTGTATCAGTACTCAAAGTACCCAACAAGCATTGCGGCACTTTCTTTTAGCCGAGATGGCCGCCTATTGGCTGTGGCTTCAAGTTATACATTTGAAGAGGGAGATAAACCCCACGAACCAGATTCCATCTTTGTTCGAAGTGTAAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTGTACCCTAATCCACCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

342

Amino Acids

37.81

Weight (kDa)

8.66

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 22, 230
AccII CGCG 1 cut(s) 33
AciI CCGC 8 cut(s) 17, 31, 73, 177, 307, 396, 863, 889
AcoI YGGCCR 1 cut(s) 886
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 3 cut(s) 23, 138, 231
AfaI GTAC 8 cut(s) 134, 186, 320, 409, 425, 839, 848, 1007
AfiI CCNNNNNNNGG 5 cut(s) 19, 26, 56, 761, 895
AflIII ACRYGT 2 cut(s) 439, 496
AgsI TTSAA 6 cut(s) 169, 559, 623, 688, 909, 923
AjiI CACGTC 1 cut(s) 499
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 3 cut(s) 8, 37, 264
AluI AGCT 3 cut(s) 8, 37, 264
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 109
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 2 cut(s) 28, 886
ApeKI GCWGC 3 cut(s) 154, 195, 329
AspA2I CCTAGG 1 cut(s) 283
AspLEI GCGC 5 cut(s) 25, 146, 227, 233, 582
AspS9I GGNCC 3 cut(s) 28, 179, 379
AsuC2I CCSGG 1 cut(s) 27
AsuII TTCGAA 1 cut(s) 964
AvaII GGWCC 2 cut(s) 179, 379
AvrII CCTAGG 1 cut(s) 283
BanI GGYRCC 2 cut(s) 22, 230
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 2 cut(s) 282, 499
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 141, 182, 341
BccI CCATC 6 cut(s) 631, 764, 782, 803, 878, 962
BceAI ACGGC 3 cut(s) 73, 190, 266
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 3 cut(s) 267, 284, 663
BfoI RGCGCY 4 cut(s) 26, 147, 228, 234
BglI GCCNNNNNGGC 1 cut(s) 885
BisI GCNGC 6 cut(s) 31, 155, 196, 330, 864, 889
BlnI CCTAGG 1 cut(s) 283
BlsI GCNGC 6 cut(s) 32, 156, 197, 331, 865, 890
BmcAI AGTACT 2 cut(s) 320, 839
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 179, 379
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 3 cut(s) 28, 179, 379
BmiI GGNNCC 5 cut(s) 24, 181, 232, 380, 381
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 69, 286
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 282, 499
BpmI CTGGAG 2 cut(s) 25, 798
Bpu10I CCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 964
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 27
BsaHI GRCGYC 3 cut(s) 23, 138, 231
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 26, 56, 761, 895
Bse118I RCCGGY 2 cut(s) 19, 227
Bse1I ACTGG 4 cut(s) 302, 352, 731, 781
Bse3DI GCAATG 1 cut(s) 858
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 2 cut(s) 279, 814
BseLI CCNNNNNNNGG 5 cut(s) 19, 26, 56, 761, 895
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 2 cut(s) 525, 669
BseNI ACTGG 4 cut(s) 302, 352, 731, 781
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 141, 182, 341
BseYI CCCAGC 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 30, 888
BshNI GGYRCC 2 cut(s) 22, 230
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 4 cut(s) 20, 26, 216, 228
BslFI GGGAC 6 cut(s) 128, 165, 392, 433, 511, 731
BslI CCNNNNNNNGG 5 cut(s) 19, 26, 56, 761, 895
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsmFI GGGAC 6 cut(s) 128, 165, 392, 433, 511, 731
BsmI GAATGC 2 cut(s) 683, 746
BsnI GGCC 2 cut(s) 30, 888
Bsp119I TTCGAA 1 cut(s) 964
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 91, 342, 649
BspACI CCGC 8 cut(s) 17, 31, 73, 177, 307, 396, 863, 889
BspANI GGCC 2 cut(s) 30, 888
BspCNI CTCAG 2 cut(s) 526, 668
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 5 cut(s) 24, 181, 232, 380, 381
BspT104I TTCGAA 1 cut(s) 964
BspT107I GGYRCC 2 cut(s) 22, 230
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 2 cut(s) 19, 227
BsrI ACTGG 4 cut(s) 302, 352, 731, 781
BssAI RCCGGY 2 cut(s) 19, 227
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 91, 342, 649
BssNI GRCGYC 3 cut(s) 23, 138, 231
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 2 cut(s) 124, 244
Bst6I CTCTTC 4 cut(s) 112, 217, 818, 918
BstACI GRCGYC 3 cut(s) 23, 138, 231
BstBI TTCGAA 1 cut(s) 964
BstC8I GCNNGC 5 cut(s) 21, 35, 142, 229, 394
BstDEI CTNAG 3 cut(s) 534, 611, 655
BstF5I GGATG 2 cut(s) 279, 814
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 4 cut(s) 26, 147, 228, 234
BstHHI GCGC 5 cut(s) 25, 146, 227, 233, 582
BstKTI GATC 3 cut(s) 94, 345, 652
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 91, 342, 649
BstMWI GCNNNNNNNGC 1 cut(s) 885
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 141, 182, 341
BstV2I GAAGAC 2 cut(s) 282, 499
BsuRI GGCC 2 cut(s) 30, 888
BtgZI GCGATG 1 cut(s) 650
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 2 cut(s) 279, 814
BtsIMutI CAGTG 3 cut(s) 240, 309, 345
Cac8I GCNNGC 5 cut(s) 21, 35, 142, 229, 394
CaiI CAGNNNCTG 1 cut(s) 710
CfoI GCGC 5 cut(s) 25, 146, 227, 233, 582
Cfr10I RCCGGY 2 cut(s) 19, 227
Cfr13I GGNCC 3 cut(s) 28, 179, 379
CseI GACGC 1 cut(s) 146
Csp6I GTAC 8 cut(s) 133, 185, 319, 408, 424, 838, 847, 1006
CviAII CATG 1 cut(s) 111
CviQI GTAC 8 cut(s) 133, 185, 319, 408, 424, 838, 847, 1006
DdeI CTNAG 3 cut(s) 534, 611, 655
DinI GGCGCC 2 cut(s) 24, 232
DpnI GATC 3 cut(s) 93, 344, 651
DpnII GATC 3 cut(s) 91, 342, 649
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 4 cut(s) 112, 217, 818, 918
EarI CTCTTC 4 cut(s) 112, 217, 818, 918
EciI GGCGGA 1 cut(s) 6
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 2 cut(s) 179, 379
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 379
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 39
EgeI GGCGCC 2 cut(s) 24, 232
EheI GGCGCC 2 cut(s) 24, 232
ErhI CCWWGG 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 1 cut(s) 114
FaqI GGGAC 6 cut(s) 128, 165, 392, 433, 511, 731
FatI CATG 1 cut(s) 110
FbaI TGATCA 1 cut(s) 342
Fnu4HI GCNGC 6 cut(s) 31, 155, 196, 330, 864, 889
FokI GGATG 2 cut(s) 266, 821
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 6 cut(s) 31, 155, 196, 330, 864, 889
FspBI CTAG 3 cut(s) 267, 284, 663
GlaI GCGC 5 cut(s) 24, 145, 226, 232, 581
GluI GCNGC 6 cut(s) 31, 155, 196, 330, 864, 889
GsaI CCCAGC 1 cut(s) 532
GsuI CTGGAG 2 cut(s) 25, 798
HaeII RGCGCY 4 cut(s) 26, 147, 228, 234
HaeIII GGCC 2 cut(s) 30, 888
HapII CCGG 4 cut(s) 20, 26, 216, 228
HgaI GACGC 1 cut(s) 146
HhaI GCGC 5 cut(s) 25, 146, 227, 233, 582
Hin1I GRCGYC 3 cut(s) 23, 138, 231
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 5 cut(s) 23, 144, 225, 231, 580
HinP1I GCGC 5 cut(s) 23, 144, 225, 231, 580
HinfI GANTC 3 cut(s) 209, 548, 950
HpaII CCGG 4 cut(s) 20, 26, 216, 228
Hpy166II GTNNAC 1 cut(s) 1006
Hpy188I TCNGA 5 cut(s) 55, 128, 658, 669, 706
Hpy8I GTNNAC 1 cut(s) 1006
Hpy99I CGWCG 3 cut(s) 59, 140, 209
HpyAV CCTTC 4 cut(s) 60, 282, 617, 708
HpyCH4III ACNGT 2 cut(s) 124, 244
HpyCH4IV ACGT 3 cut(s) 422, 439, 498
HpyCH4V TGCA 7 cut(s) 299, 329, 392, 482, 683, 740, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 885
HpyF3I CTNAG 3 cut(s) 534, 611, 655
HpySE526I ACGT 3 cut(s) 422, 439, 498
Hsp92I GRCGYC 3 cut(s) 23, 138, 231
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 5 cut(s) 23, 144, 225, 231, 580
KasI GGCGCC 2 cut(s) 22, 230
KflI GGGWCCC 1 cut(s) 379
KroI GCCGGC 2 cut(s) 19, 227
KroNI GCCGGC 2 cut(s) 21, 229
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 3 cut(s) 91, 342, 649
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 3 cut(s) 141, 182, 341
LweI GCATC 2 cut(s) 69, 286
MaeI CTAG 3 cut(s) 267, 284, 663
MaeII ACGT 3 cut(s) 422, 439, 498
MaeIII GTNAC 1 cut(s) 692
MalI GATC 3 cut(s) 93, 344, 651
MboI GATC 3 cut(s) 91, 342, 649
MboII GAAGA 7 cut(s) 99, 204, 287, 504, 543, 835, 935
MfeI CAATTG 1 cut(s) 741
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 741
Mly113I GGCGCC 2 cut(s) 23, 231
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 3 cut(s) 78, 105, 442
Mph1103I ATGCAT 1 cut(s) 685
MreI CGCCGGCG 2 cut(s) 19, 227
MroNI GCCGGC 2 cut(s) 19, 227
MslI CAYNNNNRTG 3 cut(s) 370, 501, 688
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 4 cut(s) 20, 26, 216, 228
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 683, 746
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 885
NaeI GCCGGC 2 cut(s) 21, 229
NarI GGCGCC 2 cut(s) 23, 231
NciI CCSGG 1 cut(s) 27
NdeII GATC 3 cut(s) 91, 342, 649
NgoMIV GCCGGC 2 cut(s) 19, 227
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 5 cut(s) 24, 181, 232, 380, 381
NmeAIII GCCGAG 1 cut(s) 905
NmuCI GTSAC 1 cut(s) 692
NsiI ATGCAT 1 cut(s) 685
NspV TTCGAA 1 cut(s) 964
OliI CACNNNNGTG 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 2 cut(s) 683, 746
PdiI GCCGGC 2 cut(s) 21, 229
PfeI GAWTC 2 cut(s) 548, 950
PflFI GACNNNGTC 1 cut(s) 722
PkrI GCNGC 6 cut(s) 32, 156, 197, 331, 865, 890
PleI GAGTC 1 cut(s) 203
PluTI GGCGCC 2 cut(s) 26, 234
PpsI GAGTC 1 cut(s) 203
PpuMI RGGWCCY 1 cut(s) 379
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 528
PspN4I GGNNCC 5 cut(s) 24, 181, 232, 380, 381
PspPI GGNCC 3 cut(s) 28, 179, 379
PspPPI RGGWCCY 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 710
PsyI GACNNNGTC 1 cut(s) 722
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 8 cut(s) 134, 186, 320, 409, 425, 839, 848, 1007
RsaNI GTAC 8 cut(s) 133, 185, 319, 408, 424, 838, 847, 1006
RseI CAYNNNNRTG 3 cut(s) 370, 501, 688
SacI GAGCTC 1 cut(s) 39
SatI GCNGC 6 cut(s) 31, 155, 196, 330, 864, 889
Sau3AI GATC 3 cut(s) 91, 342, 649
Sau96I GGNCC 3 cut(s) 28, 179, 379
ScaI AGTACT 2 cut(s) 320, 839
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 39
SfaNI GCATC 2 cut(s) 69, 286
SfoI GGCGCC 2 cut(s) 24, 232
SfuI TTCGAA 1 cut(s) 964
SgrAI CRCCGGYG 2 cut(s) 19, 227
SinI GGWCC 2 cut(s) 179, 379
SmiMI CAYNNNNRTG 3 cut(s) 370, 501, 688
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 1 cut(s) 741
SsiI CCGC 8 cut(s) 17, 31, 73, 177, 307, 396, 863, 889
SspDI GGCGCC 2 cut(s) 22, 230
SspMI CTAG 3 cut(s) 267, 284, 663
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 124, 244
TaiI ACGT 3 cut(s) 425, 442, 501
TaqI TCGA 3 cut(s) 189, 571, 964
TasI AATT 1 cut(s) 741
TatI WGTACW 2 cut(s) 318, 837
TauI GCSGC 3 cut(s) 33, 866, 891
TfiI GAWTC 2 cut(s) 548, 950
TscAI CASTG 3 cut(s) 247, 309, 352
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 3 cut(s) 154, 195, 329
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 2 cut(s) 99, 543
TspGWI ACGGA 1 cut(s) 556
TspRI CASTG 3 cut(s) 247, 309, 352
Tth111I GACNNNGTC 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 179, 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmaJI CCTAGG 1 cut(s) 283
XspI CTAG 3 cut(s) 267, 284, 663
ZrmI AGTACT 2 cut(s) 320, 839
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.