Rw6G005330

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012239
Physical Location & Seq
Reverse (-)
8554570 .. 8557619
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005330.1

Sequence Viewer

Length: 1026 bp
ATGACAGCTGTCCCTCCGCCGTCCTCCGGCCGAGAGCTCTCCAACCCCCCCGCCGACGGCGTCTCCAACCTCCGCTTCTCCAACCACAGCGATCACCTGCTCGTCTCCTCTTGGGATAAGACCGTCCGATTGTACGACGCCACCGCCAATGTGCTCCGCGGAGAGTTCACTCACGGCAGCCCCGTTCTCGATTGCTGCTTCCACGACGACTCCTCCGGCTTCAGCGCCAGCGCCGACAACACCGTCCGCAGATTCGTTTTCAACTCCAATAAGGAAGATATTATAGGAAAGCACGACGCACCGGTTCGTTGCGTTGAGTACTCGTATGCAGCAGGGCAACTGATCACCGGCAGTTGGGATAAGACACTCAAGTGTTGGGATCCTAGAGGGGCAAATGGACAAGAGCGTACTCTTGTTGGGACTTATCAACAGCCGGAGCGTGTTTATTCTCTTTCTCTGGTCGGAAATCGTTTAGTGGTTGCAACTGCTGGAAGACATGTGAATGTCTATGATCTGAGAAATATGTCACAGCCTGAGCAACGAAGGGAGTCTTCATTGAAGTATCAAACTCGATGTGTGCGCTGTTATCCTAACGGAACTGGTTATGCTCTTAGTTCTGTTGAAGGACGGGTTGCAATGGAATTCTTTGATCTCTCAGAGGCTAGTCAAACCAAAAAATATGCATTCAAGTGTCATAGAAAATCAGAGGCTGGAAGGGACATTGTATATCCAGTAAATGCAATTGCATTCCATCCCATATATGGTACGTTTGCTACTGGAGGTTGTGATGGCTATGTGAATGTATGGGATGGGAACAACAAAAAGCGGCTATATCAGTACTCCAAGTATCCAACGAGCATTGCAGCTCTTTCTTTTAGCAGAGACGGCCGCCTATTGGCTGTGGCTTCAAGTTACACATTTGAGGAGGGAGAAAAACCTCATGAGCCAGACGCGATCTTTGTTCGCAGTGTCAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTCTACCCTAATCCGCCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

341

Amino Acids

37.98

Weight (kDa)

8.51

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 19 - 231 1e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 19 - 232 1.1e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 19 - 128 1.5e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 19 - 165 1.5e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 20 - 127 4.2e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 75 - 304 1.2e-24 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 138 - 295 6.1e-07 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 146 - 293 2.4e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 157 - 304 2.8e-08 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 242 - 304 3.2e-06 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 247 - 303 3.1e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 105
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 2 cut(s) 159, 953
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 2 cut(s) 28, 886
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 60, 138
AfaI GTAC 5 cut(s) 134, 320, 409, 766, 839
AfiI CCNNNNNNNGG 5 cut(s) 26, 56, 354, 761, 895
AflIII ACRYGT 1 cut(s) 496
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 5 cut(s) 262, 559, 623, 688, 909
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 3 cut(s) 8, 37, 866
AluI AGCT 3 cut(s) 8, 37, 866
Alw21I GWGCWC 2 cut(s) 39, 156
Alw26I GTCTC 3 cut(s) 67, 109, 876
AlwI GGATC 2 cut(s) 374, 387
AlwNI CAGNNNCTG 1 cut(s) 710
AoxI GGCC 2 cut(s) 28, 886
ApeKI GCWGC 4 cut(s) 177, 195, 329, 863
ApoI RAATTY 1 cut(s) 641
ArsI GACNNNNNNTTYG 2 cut(s) 941, 973
AsiGI ACCGGT 1 cut(s) 301
AspLEI GCGC 3 cut(s) 227, 233, 582
AsuHPI GGTGA 2 cut(s) 86, 337
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 2 cut(s) 499, 543
Bbv12I GWGCWC 2 cut(s) 39, 156
BbvI GCAGC 4 cut(s) 182, 189, 341, 875
BccI CCATC 3 cut(s) 759, 782, 803
BceAI ACGGC 4 cut(s) 4, 73, 190, 901
BciVI GTATCC 1 cut(s) 858
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 3 cut(s) 67, 109, 876
BfaI CTAG 2 cut(s) 384, 663
BfoI RGCGCY 2 cut(s) 228, 234
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 858
BisI GCNGC 6 cut(s) 178, 196, 330, 827, 864, 889
BlsI GCNGC 6 cut(s) 179, 197, 331, 828, 865, 890
BmcAI AGTACT 2 cut(s) 320, 839
BmiI GGNNCC 1 cut(s) 381
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 499, 543
BpmI CTGGAG 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 353
BsaHI GRCGYC 2 cut(s) 60, 138
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 194, 197, 224, 227
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 56, 354, 761, 895
Bse118I RCCGGY 2 cut(s) 301, 347
Bse1I ACTGG 3 cut(s) 604, 731, 781
Bse3DI GCAATG 2 cut(s) 642, 858
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 2 cut(s) 751, 814
BseLI CCNNNNNNNGG 5 cut(s) 26, 56, 354, 761, 895
BseMI GCAATG 2 cut(s) 642, 858
BseMII CTCAG 3 cut(s) 506, 525, 669
BseNI ACTGG 3 cut(s) 604, 731, 781
BseRI GAGGAG 3 cut(s) 97, 202, 938
BseX3I CGGCCG 2 cut(s) 28, 886
BseXI GCAGC 4 cut(s) 182, 189, 341, 875
Bsh1236I CGCG 2 cut(s) 159, 953
Bsh1285I CGRYCG 2 cut(s) 31, 889
BshFI GGCC 2 cut(s) 30, 888
BshTI ACCGGT 1 cut(s) 301
BsiEI CGRYCG 2 cut(s) 31, 889
BsiHKAI GWGCWC 2 cut(s) 39, 156
BsiSI CCGG 5 cut(s) 27, 216, 302, 348, 434
BslFI GGGAC 2 cut(s) 433, 731
BslI CCNNNNNNNGG 5 cut(s) 26, 56, 354, 761, 895
BsmAI GTCTC 3 cut(s) 67, 109, 876
BsmBI CGTCTC 3 cut(s) 67, 109, 876
BsmFI GGGAC 2 cut(s) 433, 731
BsmI GAATGC 2 cut(s) 683, 746
BsnI GGCC 2 cut(s) 30, 888
Bsp1286I GDGCHC 2 cut(s) 39, 156
Bsp143I GATC 6 cut(s) 91, 342, 379, 511, 649, 954
BspANI GGCC 2 cut(s) 30, 888
BspCNI CTCAG 3 cut(s) 507, 526, 668
BspFNI CGCG 2 cut(s) 159, 953
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 374, 387
BsrDI GCAATG 2 cut(s) 642, 858
BsrFI RCCGGY 2 cut(s) 301, 347
BsrI ACTGG 3 cut(s) 604, 731, 781
BssAI RCCGGY 2 cut(s) 301, 347
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 6 cut(s) 91, 342, 379, 511, 649, 954
BssNI GRCGYC 2 cut(s) 60, 138
Bst4CI ACNGT 2 cut(s) 124, 244
BstACI GRCGYC 2 cut(s) 60, 138
BstC8I GCNNGC 2 cut(s) 229, 1020
BstDEI CTNAG 4 cut(s) 515, 534, 611, 655
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 2 cut(s) 751, 814
BstFNI CGCG 2 cut(s) 159, 953
BstH2I RGCGCY 2 cut(s) 228, 234
BstHHI GCGC 3 cut(s) 227, 233, 582
BstKTI GATC 6 cut(s) 94, 345, 382, 514, 652, 957
BstMAI GTCTC 3 cut(s) 67, 109, 876
BstMBI GATC 6 cut(s) 91, 342, 379, 511, 649, 954
BstMCI CGRYCG 2 cut(s) 31, 889
BstMWI GCNNNNNNNGC 1 cut(s) 885
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 8
BstUI CGCG 2 cut(s) 159, 953
BstV1I GCAGC 4 cut(s) 182, 189, 341, 875
BstV2I GAAGAC 2 cut(s) 499, 543
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 2 cut(s) 28, 886
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 2 cut(s) 30, 888
BtgI CCRYGG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 751, 814
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 1 cut(s) 973
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 229, 1020
CaiI CAGNNNCTG 1 cut(s) 710
CciI TCATGA 1 cut(s) 940
CfoI GCGC 3 cut(s) 227, 233, 582
Cfr10I RCCGGY 2 cut(s) 301, 347
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 4 cut(s) 49, 146, 305, 959
Csp6I GTAC 5 cut(s) 133, 319, 408, 765, 838
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 2 cut(s) 497, 941
CviQI GTAC 5 cut(s) 133, 319, 408, 765, 838
DdeI CTNAG 4 cut(s) 515, 534, 611, 655
DpnI GATC 6 cut(s) 93, 344, 381, 513, 651, 956
DpnII GATC 6 cut(s) 91, 342, 379, 511, 649, 954
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 28, 886
EagI CGGCCG 2 cut(s) 28, 886
EciI GGCGGA 2 cut(s) 6, 1005
Ecl136II GAGCTC 1 cut(s) 37
EclXI CGGCCG 2 cut(s) 28, 886
Eco24I GRGCYC 1 cut(s) 39
Eco52I CGGCCG 2 cut(s) 28, 886
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 641
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 39
Esp3I CGTCTC 3 cut(s) 67, 109, 876
FaeI CATG 2 cut(s) 500, 944
FalI AAGNNNNNCTT 2 cut(s) 535, 567
FaqI GGGAC 2 cut(s) 433, 731
FatI CATG 2 cut(s) 496, 940
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 6 cut(s) 178, 196, 330, 827, 864, 889
FokI GGATG 2 cut(s) 738, 821
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 6 cut(s) 178, 196, 330, 827, 864, 889
FspBI CTAG 2 cut(s) 384, 663
GlaI GCGC 3 cut(s) 226, 232, 581
GluI GCNGC 6 cut(s) 178, 196, 330, 827, 864, 889
GsuI CTGGAG 1 cut(s) 798
HaeII RGCGCY 2 cut(s) 228, 234
HaeIII GGCC 2 cut(s) 30, 888
HapII CCGG 5 cut(s) 27, 216, 302, 348, 434
HgaI GACGC 4 cut(s) 49, 146, 305, 959
HhaI GCGC 3 cut(s) 227, 233, 582
Hin1I GRCGYC 2 cut(s) 60, 138
Hin1II CATG 2 cut(s) 500, 944
Hin6I GCGC 3 cut(s) 225, 231, 580
HinP1I GCGC 3 cut(s) 225, 231, 580
HinfI GANTC 3 cut(s) 209, 252, 548
HpaII CCGG 5 cut(s) 27, 216, 302, 348, 434
HphI GGTGA 2 cut(s) 86, 337
Hpy166II GTNNAC 2 cut(s) 168, 1006
Hpy188I TCNGA 5 cut(s) 128, 464, 516, 658, 706
Hpy188III TCNNGA 2 cut(s) 188, 941
Hpy8I GTNNAC 2 cut(s) 168, 1006
Hpy99I CGWCG 4 cut(s) 59, 140, 209, 299
HpyAV CCTTC 3 cut(s) 537, 617, 708
HpyCH4III ACNGT 2 cut(s) 124, 244
HpyCH4IV ACGT 1 cut(s) 767
HpyCH4V TGCA 7 cut(s) 329, 482, 635, 683, 740, 746, 863
HpyF10VI GCNNNNNNNGC 1 cut(s) 885
HpyF3I CTNAG 4 cut(s) 515, 534, 611, 655
HpySE526I ACGT 1 cut(s) 767
Hsp92I GRCGYC 2 cut(s) 60, 138
Hsp92II CATG 2 cut(s) 500, 944
HspAI GCGC 3 cut(s) 225, 231, 580
Ksp22I TGATCA 1 cut(s) 342
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 6 cut(s) 91, 342, 379, 511, 649, 954
LmnI GCTCC 2 cut(s) 159, 436
Lsp1109I GCAGC 4 cut(s) 182, 189, 341, 875
MaeI CTAG 2 cut(s) 384, 663
MaeII ACGT 1 cut(s) 767
MaeIII GTNAC 2 cut(s) 525, 911
MalI GATC 6 cut(s) 93, 344, 381, 513, 651, 956
MboI GATC 6 cut(s) 91, 342, 379, 511, 649, 954
MboII GAAGA 3 cut(s) 287, 504, 543
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 39, 156
MluCI AATT 2 cut(s) 641, 741
MlyI GAGTC 2 cut(s) 203, 557
MmeI TCCRAC 5 cut(s) 66, 90, 105, 442, 875
Mph1103I ATGCAT 1 cut(s) 685
MslI CAYNNNNRTG 3 cut(s) 370, 501, 688
MspA1I CMGCKG 2 cut(s) 8, 159
MspI CCGG 5 cut(s) 27, 216, 302, 348, 434
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 683, 746
MvnI CGCG 2 cut(s) 159, 953
MwoI GCNNNNNNNGC 1 cut(s) 885
NdeII GATC 6 cut(s) 91, 342, 379, 511, 649, 954
NlaIII CATG 2 cut(s) 500, 944
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 525
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 370
PagI TCATGA 1 cut(s) 940
PaqCI CACCTGC 1 cut(s) 105
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 2 cut(s) 683, 746
PfeI GAWTC 1 cut(s) 252
PflFI GACNNNGTC 1 cut(s) 59
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 6 cut(s) 179, 197, 331, 828, 865, 890
PleI GAGTC 2 cut(s) 203, 556
PpsI GAGTC 2 cut(s) 203, 556
PscI ACATGT 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 381
PstNI CAGNNNCTG 1 cut(s) 710
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 134, 320, 409, 766, 839
RsaNI GTAC 5 cut(s) 133, 319, 408, 765, 838
RseI CAYNNNNRTG 3 cut(s) 370, 501, 688
SacI GAGCTC 1 cut(s) 39
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 6 cut(s) 178, 196, 330, 827, 864, 889
Sau3AI GATC 6 cut(s) 91, 342, 379, 511, 649, 954
ScaI AGTACT 2 cut(s) 320, 839
SchI GAGTC 2 cut(s) 203, 557
SduI GDGCHC 2 cut(s) 39, 156
SetI ASST 9 cut(s) 10, 39, 72, 99, 770, 784, 868, 940, 987
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SmiMI CAYNNNNRTG 3 cut(s) 370, 501, 688
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 641, 741
SspMI CTAG 2 cut(s) 384, 663
SstI GAGCTC 1 cut(s) 39
TaaI ACNGT 2 cut(s) 124, 244
TaiI ACGT 1 cut(s) 770
TaqI TCGA 2 cut(s) 189, 571
TasI AATT 2 cut(s) 641, 741
TatI WGTACW 2 cut(s) 318, 837
TauI GCSGC 2 cut(s) 829, 891
TfiI GAWTC 1 cut(s) 252
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 4 cut(s) 177, 195, 329, 863
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 1 cut(s) 543
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 1 cut(s) 973
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XmiI GTMKAC 1 cut(s) 1005
XspI CTAG 2 cut(s) 384, 663
ZrmI AGTACT 2 cut(s) 320, 839
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.