Rh6DG051200

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012239
Physical Location & Seq
Reverse (-)
5464833 .. 5467920
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG051200.1

Sequence Viewer

Length: 945 bp
ATGACAGCTGTCCCTCCGCCTTCCTCCGGCCGAGAGCTCTCCAACCCCCCCGCCGACGGCGTCTCCAACCTCCGCTTCTCCAACCACAGCGATCACCTGCTCGTCTCCTCATGGGATAAGACCGTCCGATTGTACGACGCCACCGCCAATGTGCTCCGCGGAGAGTTCACTCACGGCAGTCCCGTCCTCGATTGCTGCTTCCACGACGACTCCTCCGGCTTCAGCGCCAGCGCCGACAACACCGTCCGCCGATTCGTTTTCAATTCCAATAAGGAAGATATTATAGGAAAGCACGACGCACCGGTTCGTTGCGTTGAGTACTCGTATGCAGCAGGGCAACTGATCACCGGTAGTTGGGATAAGACACTCAAGTGTTGGGATCCGAGAGGGGCAAATGGACAAGAGCGTACTCTTGTTGGGACTTATCAACAGCCGGAGCGTGTTTATTCTCTTTCTCTTGTCGGAAATCGTTTAGTGGTTGCAACTGCTGGAAGACATGTGAATGTCTATGATCTGAGAAATATGTCACAACCCGAGCAACGTAGGGAATCTTCATTGAAGTATCAAACTCGATGTGTGCGCTGTTATCCTAATGGAACAGGTTATGCTCTTAGTTCTGTTGAAGGACGGGTTGCAATGGAATTCTTTGATCTCTCAGAGGCTAGTCAAACCAAAAAATACGGTACGTTTGCTACTGGAGGTTGCGATGGCTATGTGAATGTATGGGATGGGAACAACAAAAAGCGGCTATATCAGTACTCCAAGTATCCAACGAGCATTGCAGCTCTTTCTTTTAGCAGAGACGGCCGCTTATTGGCTGTGGCTTCAAGTTACACATTTGAGGAGGGAGAAAAACCTCACGAGCCAGACGCGATCTTTGTTCGCAGTGTCAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTCTACCCTAATCCGCCGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

314

Amino Acids

34.89

Weight (kDa)

8.09

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 19 - 227 2.1e-17 WDR5 beta-propeller domain
WD40_Prp19 PF24814 19 - 225 5.7e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 19 - 128 1.5e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 19 - 165 1.2e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 75 - 213 3.5e-11 THOC3 beta-propeller domain
WD40 PF00400 97 - 127 1e-05 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 227 - 277 1.1e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 105
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 924
AccII CGCG 2 cut(s) 159, 872
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 2 cut(s) 28, 805
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 60, 138
AfaI GTAC 5 cut(s) 134, 320, 409, 685, 758
AfiI CCNNNNNNNGG 4 cut(s) 26, 56, 354, 814
AflIII ACRYGT 1 cut(s) 496
AgeI ACCGGT 2 cut(s) 301, 347
AgsI TTSAA 4 cut(s) 262, 559, 623, 828
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 3 cut(s) 8, 37, 785
AluI AGCT 3 cut(s) 8, 37, 785
Alw21I GWGCWC 2 cut(s) 39, 156
Alw26I GTCTC 3 cut(s) 67, 109, 795
AlwI GGATC 2 cut(s) 374, 387
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 2 cut(s) 28, 805
ApeKI GCWGC 3 cut(s) 195, 329, 782
ApoI RAATTY 1 cut(s) 641
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
AsiGI ACCGGT 2 cut(s) 301, 347
AspLEI GCGC 3 cut(s) 227, 233, 582
AsuHPI GGTGA 2 cut(s) 86, 337
AvaI CYCGRG 1 cut(s) 533
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 535, 567
BauI CACGAG 1 cut(s) 860
BbsI GAAGAC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 39, 156
BbvI GCAGC 3 cut(s) 182, 341, 794
BccI CCATC 2 cut(s) 701, 722
BceAI ACGGC 3 cut(s) 73, 190, 820
BciVI GTATCC 1 cut(s) 777
BclI TGATCA 1 cut(s) 342
BcoDI GTCTC 3 cut(s) 67, 109, 795
BfaI CTAG 1 cut(s) 663
BfoI RGCGCY 2 cut(s) 228, 234
BfuAI ACCTGC 1 cut(s) 105
BfuI GTATCC 1 cut(s) 777
BisI GCNGC 5 cut(s) 196, 330, 746, 783, 808
BlsI GCNGC 5 cut(s) 197, 331, 747, 784, 809
BmcAI AGTACT 2 cut(s) 320, 758
BmeT110I CYCGRG 1 cut(s) 533
BmiI GGNNCC 1 cut(s) 381
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 499
BpmI CTGGAG 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 353
BsaHI GRCGYC 2 cut(s) 60, 138
BsaJI CCNNGG 1 cut(s) 157
BsaWI WCCGGW 2 cut(s) 301, 347
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 194, 197, 224, 227
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 56, 354, 814
Bse118I RCCGGY 3 cut(s) 301, 347, 937
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 2 cut(s) 642, 777
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 4 cut(s) 26, 56, 354, 814
BseMI GCAATG 2 cut(s) 642, 777
BseMII CTCAG 2 cut(s) 506, 669
BseNI ACTGG 1 cut(s) 700
BseRI GAGGAG 3 cut(s) 97, 202, 857
BseX3I CGGCCG 2 cut(s) 28, 805
BseXI GCAGC 3 cut(s) 182, 341, 794
Bsh1236I CGCG 2 cut(s) 159, 872
Bsh1285I CGRYCG 2 cut(s) 31, 808
BshFI GGCC 2 cut(s) 30, 807
BshTI ACCGGT 2 cut(s) 301, 347
BsiEI CGRYCG 2 cut(s) 31, 808
BsiHKAI GWGCWC 2 cut(s) 39, 156
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 6 cut(s) 27, 216, 302, 348, 434, 938
BslFI GGGAC 2 cut(s) 165, 433
BslI CCNNNNNNNGG 4 cut(s) 26, 56, 354, 814
BsmAI GTCTC 3 cut(s) 67, 109, 795
BsmBI CGTCTC 3 cut(s) 67, 109, 795
BsmFI GGGAC 2 cut(s) 165, 433
BsnI GGCC 2 cut(s) 30, 807
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 39, 156
Bsp143I GATC 6 cut(s) 91, 342, 379, 511, 649, 873
BspANI GGCC 2 cut(s) 30, 807
BspCNI CTCAG 2 cut(s) 507, 668
BspFNI CGCG 2 cut(s) 159, 872
BspLI GGNNCC 1 cut(s) 381
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 2 cut(s) 374, 387
BsrDI GCAATG 2 cut(s) 642, 777
BsrFI RCCGGY 3 cut(s) 301, 347, 937
BsrI ACTGG 1 cut(s) 700
BssAI RCCGGY 3 cut(s) 301, 347, 937
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 6 cut(s) 91, 342, 379, 511, 649, 873
BssNI GRCGYC 2 cut(s) 60, 138
BssSI CACGAG 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 860
Bst4CI ACNGT 3 cut(s) 124, 244, 683
BstACI GRCGYC 2 cut(s) 60, 138
BstC8I GCNNGC 2 cut(s) 229, 939
BstDEI CTNAG 3 cut(s) 515, 611, 655
BstDSI CCRYGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 2 cut(s) 159, 872
BstH2I RGCGCY 2 cut(s) 228, 234
BstHHI GCGC 3 cut(s) 227, 233, 582
BstKTI GATC 6 cut(s) 94, 345, 382, 514, 652, 876
BstMAI GTCTC 3 cut(s) 67, 109, 795
BstMBI GATC 6 cut(s) 91, 342, 379, 511, 649, 873
BstMCI CGRYCG 2 cut(s) 31, 808
BstMWI GCNNNNNNNGC 1 cut(s) 804
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 8
BstUI CGCG 2 cut(s) 159, 872
BstV1I GCAGC 3 cut(s) 182, 341, 794
BstV2I GAAGAC 1 cut(s) 499
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 2 cut(s) 28, 805
BsuI GTATCC 1 cut(s) 777
BsuRI GGCC 2 cut(s) 30, 807
BtgI CCRYGG 1 cut(s) 157
BtgZI GCGATG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 1 cut(s) 892
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 2 cut(s) 229, 939
CfoI GCGC 3 cut(s) 227, 233, 582
Cfr10I RCCGGY 3 cut(s) 301, 347, 937
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 4 cut(s) 49, 146, 305, 878
Csp6I GTAC 5 cut(s) 133, 319, 408, 684, 757
CspAI ACCGGT 2 cut(s) 301, 347
CviAII CATG 2 cut(s) 111, 497
CviQI GTAC 5 cut(s) 133, 319, 408, 684, 757
DdeI CTNAG 3 cut(s) 515, 611, 655
DpnI GATC 6 cut(s) 93, 344, 381, 513, 651, 875
DpnII GATC 6 cut(s) 91, 342, 379, 511, 649, 873
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 2 cut(s) 28, 805
EagI CGGCCG 2 cut(s) 28, 805
EciI GGCGGA 3 cut(s) 6, 236, 924
Ecl136II GAGCTC 1 cut(s) 37
EclXI CGGCCG 2 cut(s) 28, 805
Eco24I GRGCYC 1 cut(s) 39
Eco52I CGGCCG 2 cut(s) 28, 805
Eco53kI GAGCTC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 641
EcoT38I GRGCYC 1 cut(s) 39
Esp3I CGTCTC 3 cut(s) 67, 109, 795
FaeI CATG 2 cut(s) 114, 500
FaqI GGGAC 2 cut(s) 165, 433
FatI CATG 2 cut(s) 110, 496
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 5 cut(s) 196, 330, 746, 783, 808
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 5 cut(s) 196, 330, 746, 783, 808
FspBI CTAG 1 cut(s) 663
GlaI GCGC 3 cut(s) 226, 232, 581
GluI GCNGC 5 cut(s) 196, 330, 746, 783, 808
GsuI CTGGAG 1 cut(s) 717
HaeII RGCGCY 2 cut(s) 228, 234
HaeIII GGCC 2 cut(s) 30, 807
HapII CCGG 6 cut(s) 27, 216, 302, 348, 434, 938
HgaI GACGC 4 cut(s) 49, 146, 305, 878
HhaI GCGC 3 cut(s) 227, 233, 582
Hin1I GRCGYC 2 cut(s) 60, 138
Hin1II CATG 2 cut(s) 114, 500
Hin6I GCGC 3 cut(s) 225, 231, 580
HinP1I GCGC 3 cut(s) 225, 231, 580
HinfI GANTC 3 cut(s) 209, 252, 548
HpaII CCGG 6 cut(s) 27, 216, 302, 348, 434, 938
HphI GGTGA 2 cut(s) 86, 337
Hpy166II GTNNAC 2 cut(s) 168, 925
Hpy188I TCNGA 5 cut(s) 128, 384, 464, 516, 658
Hpy188III TCNNGA 1 cut(s) 860
Hpy8I GTNNAC 2 cut(s) 168, 925
Hpy99I CGWCG 4 cut(s) 59, 140, 209, 299
HpyAV CCTTC 2 cut(s) 30, 617
HpyCH4III ACNGT 3 cut(s) 124, 244, 683
HpyCH4IV ACGT 2 cut(s) 541, 686
HpyCH4V TGCA 4 cut(s) 329, 482, 635, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 804
HpyF3I CTNAG 3 cut(s) 515, 611, 655
HpySE526I ACGT 2 cut(s) 541, 686
Hsp92I GRCGYC 2 cut(s) 60, 138
Hsp92II CATG 2 cut(s) 114, 500
HspAI GCGC 3 cut(s) 225, 231, 580
KroI GCCGGC 1 cut(s) 937
KroNI GCCGGC 1 cut(s) 939
Ksp22I TGATCA 1 cut(s) 342
KspI CCGCGG 1 cut(s) 160
Kzo9I GATC 6 cut(s) 91, 342, 379, 511, 649, 873
LmnI GCTCC 2 cut(s) 159, 436
Lsp1109I GCAGC 3 cut(s) 182, 341, 794
MaeI CTAG 1 cut(s) 663
MaeII ACGT 2 cut(s) 541, 686
MaeIII GTNAC 2 cut(s) 525, 830
MalI GATC 6 cut(s) 93, 344, 381, 513, 651, 875
MboI GATC 6 cut(s) 91, 342, 379, 511, 649, 873
MboII GAAGA 3 cut(s) 287, 504, 543
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 39, 156
MluCI AATT 2 cut(s) 262, 641
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 5 cut(s) 66, 90, 105, 442, 794
MroNI GCCGGC 1 cut(s) 937
MslI CAYNNNNRTG 2 cut(s) 370, 501
MspA1I CMGCKG 2 cut(s) 8, 159
MspI CCGG 6 cut(s) 27, 216, 302, 348, 434, 938
MvnI CGCG 2 cut(s) 159, 872
MwoI GCNNNNNNNGC 1 cut(s) 804
NaeI GCCGGC 1 cut(s) 939
NdeII GATC 6 cut(s) 91, 342, 379, 511, 649, 873
NgoMIV GCCGGC 1 cut(s) 937
NlaIII CATG 2 cut(s) 114, 500
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 525
NspI RCATGY 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 370
PaqCI CACCTGC 1 cut(s) 105
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PdiI GCCGGC 1 cut(s) 939
PfeI GAWTC 2 cut(s) 252, 548
PflFI GACNNNGTC 1 cut(s) 59
PinAI ACCGGT 2 cut(s) 301, 347
PkrI GCNGC 5 cut(s) 197, 331, 747, 784, 809
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PscI ACATGT 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 381
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 134, 320, 409, 685, 758
RsaNI GTAC 5 cut(s) 133, 319, 408, 684, 757
RseI CAYNNNNRTG 2 cut(s) 370, 501
SacI GAGCTC 1 cut(s) 39
SacII CCGCGG 1 cut(s) 160
SatI GCNGC 5 cut(s) 196, 330, 746, 783, 808
Sau3AI GATC 6 cut(s) 91, 342, 379, 511, 649, 873
ScaI AGTACT 2 cut(s) 320, 758
SchI GAGTC 1 cut(s) 203
SduI GDGCHC 2 cut(s) 39, 156
Sfr303I CCGCGG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 370, 501
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 262, 641
SspMI CTAG 1 cut(s) 663
SstI GAGCTC 1 cut(s) 39
TaaI ACNGT 3 cut(s) 124, 244, 683
TaiI ACGT 2 cut(s) 544, 689
TaqI TCGA 2 cut(s) 189, 571
TasI AATT 2 cut(s) 262, 641
TatI WGTACW 2 cut(s) 318, 756
TauI GCSGC 2 cut(s) 748, 810
TfiI GAWTC 2 cut(s) 252, 548
TscAI CASTG 1 cut(s) 892
TseFI GTSAC 1 cut(s) 525
TseI GCWGC 3 cut(s) 195, 329, 782
Tsp45I GTSAC 1 cut(s) 525
TspDTI ATGAA 1 cut(s) 543
TspRI CASTG 1 cut(s) 892
Tth111I GACNNNGTC 1 cut(s) 59
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 924
XspI CTAG 1 cut(s) 663
ZrmI AGTACT 2 cut(s) 320, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.