Rroxscaffold_7G00211810

Mitotic checkpoint protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012239
Physical Location & Seq
Forward (+)
62220619 .. 62223739
3121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00211810.1

Sequence Viewer

Length: 756 bp
ATGTGCTCCGCGGAGAGTTCACGCACGGCAGCCCCGTCCTCGATTGCTGCTTCCACGACGACTCCTCTGGCTTCAGCGCCAGCGCCGACAACACCATTCGTTTTCAACTCCAATAAGGAAGATATTATAGGAAAGCACGACGCACCGGTTCGTTGCGTTGAGTACTCGTATGCAGCAGTTTACAGTAATCGCATTTATAGGGCAAATGGACAAGAGCGTACTCTTGTTGGGACTTATCAACAGCCGGAGCGTGTTTATTCGCTTTCTCTGGTTGGAAATCGTTTAGTGGTTGCAACTGCTGGAAGACATGTGAATGTCTATGATCTGAGAAATATGTCACAGCCTGAGCAACGAAGGGAATCTTCATTGAAGTATCAAACTCGATGTGTGCGCTGTTATCCTAATGGAACAGGTTATGCTCTTAGTTCTGTCGAAGGACGGGTTGCAATGGAATTCTTTGATCTCTCAGAGGCTAGTCAAACCAAAAAATACGGTACGTTTGCTACTGGAGGTTGTGATGGCTATGTGAATGTATGGGATGGGAACAACAAAAAGCGGCTATATCAGTACTCCAAGTATCCAACGAGCATTGCAGCTCTTTCTTTTAGCAGAGACGGCCGCCTATTGGCTGTGGCTTCAAGTTACACATTTGAGGAGGGAGAAAAACCTCATGAGCCAGACGCGATCTTTGTTCGCAGTGTCAATGAGATAGAGGTCAAGCCAAAGCCAAAAGTCTACCCTAATCCGCCAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000075 GO:0000151 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000779 GO:0000793 GO:0000819 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006996 GO:0007049 GO:0007059 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0031974 GO:0031981 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051276 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0070013 GO:0071173 GO:0071174 GO:0071840 GO:0080008 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
WD40

Protein Analysis

251

Amino Acids

27.76

Weight (kDa)

8.98

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 164 - 214 7.3e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012239)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49910 AT3G19590
fragaria_vesca FvH4_2g04170 FvH4_2g04170
malus_domestica MD05G1098500.v1.1 MD10G1104300.v1.1
prunus_persica Prupe.8G143300_v2.0.a1
pyrus_communis pycom05g09640 pycom10g08990
rosa_chinensis RchiOBHm_Chr6g0253521
rosa_laevigata RLG00000015012
rosa_multiflora Rmu_ssc0000079.1_g000004
rosa_roxburghii Rroxscaffold_7G00211810
rosa_rugosa Rorug05G0544700
rosa_samantha Rh6AG059900 Rh6BG053900 Rh6CG053600 Rh6DG051200
rosa_wichuraiana Rw6G005330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 735
AccII CGCG 2 cut(s) 11, 683
AciI CCGC 5 cut(s) 9, 11, 556, 619, 746
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 4 cut(s) 164, 220, 496, 569
AfiI CCNNNNNNNGG 1 cut(s) 625
AflIII ACRYGT 1 cut(s) 307
AgeI ACCGGT 1 cut(s) 145
AgsI TTSAA 3 cut(s) 106, 370, 639
AluBI AGCT 1 cut(s) 596
AluI AGCT 1 cut(s) 596
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 606
AoxI GGCC 1 cut(s) 616
ApeKI GCWGC 4 cut(s) 29, 47, 173, 593
ApoI RAATTY 1 cut(s) 452
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AsiGI ACCGGT 1 cut(s) 145
AspLEI GCGC 3 cut(s) 79, 85, 393
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 34, 41, 185, 605
BccI CCATC 2 cut(s) 512, 533
BceAI ACGGC 2 cut(s) 42, 631
BciVI GTATCC 1 cut(s) 588
BcoDI GTCTC 1 cut(s) 606
BfaI CTAG 1 cut(s) 474
BfoI RGCGCY 2 cut(s) 80, 86
BfuI GTATCC 1 cut(s) 588
BisI GCNGC 6 cut(s) 30, 48, 174, 557, 594, 619
BlsI GCNGC 6 cut(s) 31, 49, 175, 558, 595, 620
BmcAI AGTACT 2 cut(s) 164, 569
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 9
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 1 cut(s) 511
Bse3DI GCAATG 2 cut(s) 453, 588
BseDI CCNNGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMI GCAATG 2 cut(s) 453, 588
BseMII CTCAG 3 cut(s) 317, 336, 480
BseNI ACTGG 1 cut(s) 511
BseRI GAGGAG 2 cut(s) 54, 668
BseX3I CGGCCG 1 cut(s) 616
BseXI GCAGC 4 cut(s) 34, 41, 185, 605
Bsh1236I CGCG 2 cut(s) 11, 683
Bsh1285I CGRYCG 1 cut(s) 619
BshFI GGCC 1 cut(s) 618
BshTI ACCGGT 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 2 cut(s) 146, 245
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 1 cut(s) 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 1 cut(s) 244
BsnI GGCC 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 3 cut(s) 322, 460, 684
BspACI CCGC 5 cut(s) 9, 11, 556, 619, 746
BspANI GGCC 1 cut(s) 618
BspCNI CTCAG 3 cut(s) 318, 337, 479
BspFNI CGCG 2 cut(s) 11, 683
BspHI TCATGA 1 cut(s) 670
BsrDI GCAATG 2 cut(s) 453, 588
BsrFI RCCGGY 1 cut(s) 145
BsrI ACTGG 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 9
BssMI GATC 3 cut(s) 322, 460, 684
Bst4CI ACNGT 2 cut(s) 185, 494
BstC8I GCNNGC 2 cut(s) 81, 750
BstDEI CTNAG 4 cut(s) 326, 345, 422, 466
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 2 cut(s) 11, 683
BstH2I RGCGCY 2 cut(s) 80, 86
BstHHI GCGC 3 cut(s) 79, 85, 393
BstKTI GATC 3 cut(s) 325, 463, 687
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 3 cut(s) 322, 460, 684
BstMCI CGRYCG 1 cut(s) 619
BstMWI GCNNNNNNNGC 1 cut(s) 615
BstNSI RCATGY 1 cut(s) 311
BstUI CGCG 2 cut(s) 11, 683
BstV1I GCAGC 4 cut(s) 34, 41, 185, 605
BstV2I GAAGAC 1 cut(s) 310
BstZI CGGCCG 1 cut(s) 616
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 1 cut(s) 618
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 544
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 81, 750
CciI TCATGA 1 cut(s) 670
CfoI GCGC 3 cut(s) 79, 85, 393
Cfr10I RCCGGY 1 cut(s) 145
Cfr42I CCGCGG 1 cut(s) 12
CseI GACGC 2 cut(s) 149, 689
Csp6I GTAC 4 cut(s) 163, 219, 495, 568
CspAI ACCGGT 1 cut(s) 145
CviAII CATG 2 cut(s) 308, 671
CviQI GTAC 4 cut(s) 163, 219, 495, 568
DdeI CTNAG 4 cut(s) 326, 345, 422, 466
DpnI GATC 3 cut(s) 324, 462, 686
DpnII GATC 3 cut(s) 322, 460, 684
EaeI YGGCCR 1 cut(s) 616
EagI CGGCCG 1 cut(s) 616
EciI GGCGGA 1 cut(s) 735
EclXI CGGCCG 1 cut(s) 616
Eco52I CGGCCG 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 57
EcoRI GAATTC 1 cut(s) 452
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 2 cut(s) 311, 674
FalI AAGNNNNNCTT 2 cut(s) 346, 378
FaqI GGGAC 1 cut(s) 244
FatI CATG 2 cut(s) 307, 670
FblI GTMKAC 1 cut(s) 735
Fnu4HI GCNGC 6 cut(s) 30, 48, 174, 557, 594, 619
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 6 cut(s) 30, 48, 174, 557, 594, 619
FspBI CTAG 1 cut(s) 474
GlaI GCGC 3 cut(s) 78, 84, 392
GluI GCNGC 6 cut(s) 30, 48, 174, 557, 594, 619
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 2 cut(s) 80, 86
HaeIII GGCC 1 cut(s) 618
HapII CCGG 2 cut(s) 146, 245
HgaI GACGC 2 cut(s) 149, 689
HhaI GCGC 3 cut(s) 79, 85, 393
Hin1II CATG 2 cut(s) 311, 674
Hin6I GCGC 3 cut(s) 77, 83, 391
HinP1I GCGC 3 cut(s) 77, 83, 391
HinfI GANTC 2 cut(s) 61, 359
HpaII CCGG 2 cut(s) 146, 245
Hpy166II GTNNAC 3 cut(s) 20, 181, 736
Hpy188I TCNGA 2 cut(s) 327, 469
Hpy188III TCNNGA 1 cut(s) 671
Hpy8I GTNNAC 3 cut(s) 20, 181, 736
Hpy99I CGWCG 2 cut(s) 61, 143
HpyAV CCTTC 2 cut(s) 348, 428
HpyCH4III ACNGT 2 cut(s) 185, 494
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 4 cut(s) 173, 293, 446, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 615
HpyF3I CTNAG 4 cut(s) 326, 345, 422, 466
HpySE526I ACGT 1 cut(s) 497
Hsp92II CATG 2 cut(s) 311, 674
HspAI GCGC 3 cut(s) 77, 83, 391
KspI CCGCGG 1 cut(s) 12
Kzo9I GATC 3 cut(s) 322, 460, 684
LmnI GCTCC 2 cut(s) 11, 247
Lsp1109I GCAGC 4 cut(s) 34, 41, 185, 605
MaeI CTAG 1 cut(s) 474
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 2 cut(s) 336, 641
MalI GATC 3 cut(s) 324, 462, 686
MboI GATC 3 cut(s) 322, 460, 684
MboII GAAGA 3 cut(s) 131, 315, 354
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 1 cut(s) 452
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 2 cut(s) 253, 605
MnlI CCTC 8 cut(s) 49, 75, 463, 503, 646, 649, 678, 706
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 11
MspI CCGG 2 cut(s) 146, 245
MvnI CGCG 2 cut(s) 11, 683
MwoI GCNNNNNNNGC 1 cut(s) 615
NdeII GATC 3 cut(s) 322, 460, 684
NlaIII CATG 2 cut(s) 311, 674
NmuCI GTSAC 1 cut(s) 336
NspI RCATGY 1 cut(s) 311
PagI TCATGA 1 cut(s) 670
PciI ACATGT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PfeI GAWTC 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 145
PkrI GCNGC 6 cut(s) 31, 49, 175, 558, 595, 620
PleI GAGTC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 55
PscI ACATGT 1 cut(s) 307
RsaI GTAC 4 cut(s) 164, 220, 496, 569
RsaNI GTAC 4 cut(s) 163, 219, 495, 568
RseI CAYNNNNRTG 1 cut(s) 312
SacII CCGCGG 1 cut(s) 12
SatI GCNGC 6 cut(s) 30, 48, 174, 557, 594, 619
Sau3AI GATC 3 cut(s) 322, 460, 684
ScaI AGTACT 2 cut(s) 164, 569
SchI GAGTC 1 cut(s) 55
SduI GDGCHC 1 cut(s) 8
SetI ASST 6 cut(s) 415, 500, 514, 598, 670, 717
Sfr303I CCGCGG 1 cut(s) 12
SgrBI CCGCGG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 312
Sse9I AATT 1 cut(s) 452
SsiI CCGC 5 cut(s) 9, 11, 556, 619, 746
SspMI CTAG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 185, 494
TaiI ACGT 1 cut(s) 500
TaqI TCGA 3 cut(s) 41, 382, 432
TasI AATT 1 cut(s) 452
TatI WGTACW 2 cut(s) 162, 567
TauI GCSGC 2 cut(s) 559, 621
TfiI GAWTC 1 cut(s) 359
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 336
TseI GCWGC 4 cut(s) 29, 47, 173, 593
Tsp45I GTSAC 1 cut(s) 336
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 1 cut(s) 703
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 311
XmiI GTMKAC 1 cut(s) 735
XspI CTAG 1 cut(s) 474
ZrmI AGTACT 2 cut(s) 164, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.