Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005263

Physical Location & Seq

Genomic Coordinates Forward (+)

19199351 .. 19201707

Gene Structure 2357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G51760.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGAGTTTCTTCAAATGGGTTTCTTTTGTTTTGATCCTTCACTTGCTTAATCCGACTCTGATTTCATGTTCCTCTAATGGGTTATCTCAAATACCTTCAAAGTTTCTTACTTTGGCTAAAAGGAATGATTTTTTCGATTGGATGGTTGGAATCAGAAGGAGAATCCATGAGAATCCAGAGTTAGGTTATGAGGAAGTAGAGACATCTAAGCTTGTTAGAGCAGAGTTGGAGAAGATGGGTGTTTCTTATAAGTACCCAGTTGCTGTTACTGGTGTTGTTGGGTATGTTGGAACTGGTCATGCTCCTTTTGTTGCTTTAAGGGCTGATATGGATGCACTTGCTATGCAGGAAATGGTGGAATGGGAACACAAGAGTAAGGTTCCAGGGAAGATGCACGCTTGTGGACATGACGCTCACACTACGATGCTTCTCGGTGCTGCAAAATTGCTCAAAGAACATGAAGAAGAGCTACAGGGCACAGTGGTTCTAGTTTTCCAACCAGCTGAGGAAGGAGGAGGAGGTGCAAAGAAGATTGTGGAGGCTGGAGTGTTGGAGAATGTGAGTGCAATCTTTGGATTACATGTCACAAATCAATTGGCATTAGGTCAAGTGAGCTCGAGAGAGGGTCCTATGTTGGCTGGTAGTGGCTTTTTCAAAGCTAAGATAAGCGGGAAAGGAGGCCACGCAGCTCTTCCGCAGCATACCATAGATCCGATACTGGCAGCTTCAAATGTTATTGTTAGCTTACAACACCTCGTTTCACGAGAGGCAGATCCTTTAGACTCTCAGGTGGTTACAGTTGCTAAATTTGAAGGCGGTGGTGCTTTCAATGTGATTCCTGACTCTGTTACTATCGGTGGTACATTCCGAGCTTTTTCAACTAAAAGCTTTATGCAACTCAAGAAAAGAATTGAGCAGGTTATCACAAGGCAAGCGAGCGTGAATATGTGTAATGCAACAGTGGATTTCATTGAAGAGGAGAAACCTTTTTTCCCACCAACTGTAAATGACAAAGCCTTACACCAGTTTTTCAAGAACGTTTCAGGCGATATGTTAGGGATTGAGAATTACGTCGAGATGCAACCATTAATGGGATCAGAAGATTTCTCTTTCTACCAACAAGCAATCCCGGGACATTTTAGCTTCGTTGGAATGCAAAACAAAGCTCGTTCACCAATGGCGAGCCCTCACTCGCCGTACTTTGAAGTTAACGAAGAATTACTACCTTATGGTGCTTCCCTTCATGCTTCCATGGCCACAAGATATCTTCTTGAACTAAAAGCTTCAACTCTAAATAAGAGTAACAAAAAAGATGAACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005886 GO:0006950 GO:0008150 GO:0008152 GO:0009611 GO:0009850 GO:0009987 GO:0010112 GO:0010178 GO:0010179 GO:0010817 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0031347 GO:0032101 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0065008 GO:0071944 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

440

Amino Acids

48.26

Weight (kDa)

6.25

Isoelectric Point (pI)

40.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	105 - 420	1.5e-44	Peptidase family M20/M25/M40
M20_dimer	PF07687	214 - 311	1.3e-11	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005263)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51760 AT1G51780
fragaria_vesca	FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830
malus_domestica	MD09G1197800.v1.1 MD17G1178700.v1.1
prunus_persica	Prupe.3G053700_v2.0.a1
pyrus_communis	pycom17g18780
rosa_chinensis	RchiOBHm_Chr2g0142041
rosa_laevigata	RLG00000019947
rosa_multiflora	Rmu_sc0004757.1_g000007
rosa_roxburghii	Rroxscaffold_2G00103650
rosa_rugosa	Rorug02G0369800 Rorug02G0369900 Rorug02G0370000
rosa_samantha	Rh2AG422500 Rh2BG432700 Rh2CG409400 Rh2DG442400
rosa_wichuraiana	Rw2G034620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	249
Acc36I	ACCTGC	1 cut(s)	907
AciI	CCGC	3 cut(s)	669, 695, 816
AclI	AACGTT	1 cut(s)	1038
AclWI	GGATC	4 cut(s)	28, 704, 767, 1102
AcoI	YGGCCR	1 cut(s)	1254
AcsI	RAATTY	1 cut(s)	806
AfaI	GTAC	3 cut(s)	254, 862, 1199
AfiI	CCNNNNNNNGG	3 cut(s)	78, 182, 1091
AflIII	ACRYGT	1 cut(s)	580
AjnI	CCWGG	1 cut(s)	382
AleI	CACNNNNGTG	1 cut(s)	399
Alw21I	GWGCWC	1 cut(s)	617
Alw26I	GTCTC	1 cut(s)	194
AlwI	GGATC	4 cut(s)	28, 704, 767, 1102
AlwNI	CAGNNNCTG	1 cut(s)	263
Ama87I	CYCGRG	2 cut(s)	616, 1129
AoxI	GGCC	2 cut(s)	679, 1254
ApeKI	GCWGC	4 cut(s)	437, 686, 697, 722
ApoI	RAATTY	1 cut(s)	806
AseI	ATTAAT	1 cut(s)	1088
AspS9I	GGNCC	1 cut(s)	626
AsuC2I	CCSGG	2 cut(s)	1130, 1131
AsuHPI	GGTGA	1 cut(s)	1164
AvaI	CYCGRG	2 cut(s)	616, 1129
AvaII	GGWCC	1 cut(s)	626
BaeGI	GKGCMC	1 cut(s)	479
BalI	TGGCCA	1 cut(s)	1256
BanII	GRGCYC	2 cut(s)	617, 1187
BarI	GAAGNNNNNNTAC	4 cut(s)	453, 485, 1206, 1238
BauI	CACGAG	1 cut(s)	762
Bbv12I	GWGCWC	1 cut(s)	617
BbvCI	CCTCAGC	1 cut(s)	504
BbvI	GCAGC	4 cut(s)	424, 698, 709, 734
BccI	CCATC	2 cut(s)	136, 229
BceAI	ACGGC	1 cut(s)	1180
BciT130I	CCWGG	1 cut(s)	384
BcnI	CCSGG	2 cut(s)	1130, 1131
BcoDI	GTCTC	1 cut(s)	194
BfaI	CTAG	1 cut(s)	488
BfmI	CTRYAG	1 cut(s)	470
BfuAI	ACCTGC	1 cut(s)	907
BisI	GCNGC	4 cut(s)	438, 687, 698, 723
BlsI	GCNGC	4 cut(s)	439, 688, 699, 724
Bme1390I	CCNGG	3 cut(s)	384, 1130, 1131
Bme18I	GGWCC	1 cut(s)	626
BmeT110I	CYCGRG	2 cut(s)	616, 1129
BmgT120I	GGNCC	1 cut(s)	626
BmiI	GGNNCC	2 cut(s)	381, 627
BmrFI	CCNGG	3 cut(s)	384, 1130, 1131
BmrI	ACTGGG	1 cut(s)	251
BmsI	GCATC	4 cut(s)	322, 381, 414, 1068
BmuI	ACTGGG	1 cut(s)	251
BpmI	CTGGAG	1 cut(s)	564
Bpu10I	CCTNAGC	1 cut(s)	504
BpuEI	CTTGAG	1 cut(s)	884
BpuMI	CCSGG	2 cut(s)	1130, 1131
BsaJI	CCNNGG	3 cut(s)	383, 1129, 1251
Bsc4I	CCNNNNNNNGG	3 cut(s)	78, 182, 1091
Bse1I	ACTGG	5 cut(s)	257, 274, 298, 723, 1024
BseBI	CCWGG	1 cut(s)	384
BseDI	CCNNGG	3 cut(s)	383, 1129, 1251
BseGI	GGATG	2 cut(s)	147, 337
BseLI	CCNNNNNNNGG	3 cut(s)	78, 182, 1091
BseMII	CTCAG	2 cut(s)	495, 800
BseNI	ACTGG	5 cut(s)	257, 274, 298, 723, 1024
BseRI	GAGGAG	3 cut(s)	528, 531, 992
BseSI	GKGCMC	1 cut(s)	479
BseXI	GCAGC	4 cut(s)	424, 698, 709, 734
BshFI	GGCC	2 cut(s)	681, 1256
BsiHKAI	GWGCWC	1 cut(s)	617
BsiHKCI	CYCGRG	2 cut(s)	616, 1129
BsiSI	CCGG	1 cut(s)	1130
BslFI	GGGAC	1 cut(s)	1146
BslI	CCNNNNNNNGG	3 cut(s)	78, 182, 1091
BsmAI	GTCTC	1 cut(s)	194
BsmFI	GGGAC	1 cut(s)	1146
BsmI	GAATGC	1 cut(s)	1158
BsnI	GGCC	2 cut(s)	681, 1256
BsoBI	CYCGRG	2 cut(s)	616, 1129
Bsp1286I	GDGCHC	3 cut(s)	479, 617, 1187
Bsp143I	GATC	4 cut(s)	33, 709, 772, 1094
Bsp19I	CCATGG	1 cut(s)	1251
BspACI	CCGC	3 cut(s)	669, 695, 816
BspANI	GGCC	2 cut(s)	681, 1256
BspCNI	CTCAG	2 cut(s)	496, 799
BspLI	GGNNCC	2 cut(s)	381, 627
BspMI	ACCTGC	1 cut(s)	907
BspPI	GGATC	4 cut(s)	28, 704, 767, 1102
BspQI	GCTCTTC	2 cut(s)	459, 696
BsrI	ACTGG	5 cut(s)	257, 274, 298, 723, 1024
BssECI	CCNNGG	3 cut(s)	383, 1129, 1251
BssMI	GATC	4 cut(s)	33, 709, 772, 1094
BssSI	CACGAG	1 cut(s)	762
BssT1I	CCWWGG	1 cut(s)	1251
Bst2BI	CACGAG	1 cut(s)	762
Bst2UI	CCWGG	1 cut(s)	384
Bst4CI	ACNGT	4 cut(s)	481, 799, 961, 1003
Bst6I	CTCTTC	3 cut(s)	459, 696, 969
BstC8I	GCNNGC	4 cut(s)	396, 933, 937, 1183
BstDEI	CTNAG	4 cut(s)	207, 504, 660, 786
BstDSI	CCRYGG	1 cut(s)	1251
BstF5I	GGATG	2 cut(s)	147, 337
BstKTI	GATC	4 cut(s)	36, 712, 775, 1097
BstMAI	GTCTC	1 cut(s)	194
BstMBI	GATC	4 cut(s)	33, 709, 772, 1094
BstMWI	GCNNNNNNNGC	2 cut(s)	320, 1253
BstNI	CCWGG	1 cut(s)	384
BstNSI	RCATGY	1 cut(s)	584
BstSCI	CCNGG	3 cut(s)	382, 1128, 1129
BstSFI	CTRYAG	1 cut(s)	470
BstSLI	GKGCMC	1 cut(s)	479
BstV1I	GCAGC	4 cut(s)	424, 698, 709, 734
BstX2I	RGATCY	2 cut(s)	709, 772
BstYI	RGATCY	2 cut(s)	709, 772
BsuRI	GGCC	2 cut(s)	681, 1256
BtgI	CCRYGG	1 cut(s)	1251
BtsCI	GGATG	2 cut(s)	147, 337
BtsIMutI	CAGTG	2 cut(s)	486, 966
BveI	ACCTGC	1 cut(s)	907
Cac8I	GCNNGC	4 cut(s)	396, 933, 937, 1183
CaiI	CAGNNNCTG	1 cut(s)	263
Cfr13I	GGNCC	1 cut(s)	626
Cfr9I	CCCGGG	1 cut(s)	1129
CseI	GACGC	1 cut(s)	419
Csp6I	GTAC	3 cut(s)	253, 861, 1198
CviAII	CATG	8 cut(s)	66, 167, 299, 407, 458, 581, 1244, 1252
CviQI	GTAC	3 cut(s)	253, 861, 1198
DdeI	CTNAG	4 cut(s)	207, 504, 660, 786
DpnI	GATC	4 cut(s)	35, 711, 774, 1096
DpnII	GATC	4 cut(s)	33, 709, 772, 1094
EaeI	YGGCCR	1 cut(s)	1254
Eam1104I	CTCTTC	3 cut(s)	459, 696, 969
EarI	CTCTTC	3 cut(s)	459, 696, 969
Ecl136II	GAGCTC	1 cut(s)	615
Eco130I	CCWWGG	1 cut(s)	1251
Eco24I	GRGCYC	2 cut(s)	617, 1187
Eco32I	GATATC	1 cut(s)	1265
Eco47I	GGWCC	1 cut(s)	626
Eco53kI	GAGCTC	1 cut(s)	615
Eco88I	CYCGRG	2 cut(s)	616, 1129
EcoICRI	GAGCTC	1 cut(s)	615
EcoO109I	RGGNCCY	1 cut(s)	626
EcoRII	CCWGG	1 cut(s)	382
EcoRV	GATATC	1 cut(s)	1265
EcoT14I	CCWWGG	1 cut(s)	1251
EcoT38I	GRGCYC	2 cut(s)	617, 1187
ErhI	CCWWGG	1 cut(s)	1251
FaeI	CATG	8 cut(s)	69, 170, 302, 410, 461, 584, 1247, 1255
FalI	AAGNNNNNCTT	1 cut(s)	1302
FaqI	GGGAC	1 cut(s)	1146
FatI	CATG	8 cut(s)	65, 166, 298, 406, 457, 580, 1243, 1251
FauI	CCCGC	1 cut(s)	662
Fnu4HI	GCNGC	4 cut(s)	438, 687, 698, 723
FokI	GGATG	2 cut(s)	154, 344
FriOI	GRGCYC	2 cut(s)	617, 1187
Fsp4HI	GCNGC	4 cut(s)	438, 687, 698, 723
FspBI	CTAG	1 cut(s)	488
GluI	GCNGC	4 cut(s)	438, 687, 698, 723
GsuI	CTGGAG	1 cut(s)	564
HaeIII	GGCC	2 cut(s)	681, 1256
HapII	CCGG	1 cut(s)	1130
HgaI	GACGC	1 cut(s)	419
Hin1II	CATG	8 cut(s)	69, 170, 302, 410, 461, 584, 1247, 1255
HincII	GTYRAC	1 cut(s)	1210
HindII	GTYRAC	1 cut(s)	1210
HindIII	AAGCTT	3 cut(s)	209, 886, 1281
HinfI	GANTC	7 cut(s)	55, 150, 162, 172, 782, 835, 842
HpaI	GTTAAC	1 cut(s)	1210
HpaII	CCGG	1 cut(s)	1130
HphI	GGTGA	1 cut(s)	1164
Hpy166II	GTNNAC	3 cut(s)	404, 1172, 1210
Hpy188I	TCNGA	6 cut(s)	54, 60, 155, 714, 869, 1099
Hpy188III	TCNNGA	8 cut(s)	176, 618, 762, 839, 901, 1033, 1075, 1271
Hpy8I	GTNNAC	3 cut(s)	404, 1172, 1210
Hpy99I	CGWCG	1 cut(s)	1076
HpyAV	CCTTC	6 cut(s)	47, 105, 150, 503, 806, 1250
HpyCH4III	ACNGT	4 cut(s)	481, 799, 961, 1003
HpyCH4IV	ACGT	2 cut(s)	1038, 1071
HpyF10VI	GCNNNNNNNGC	2 cut(s)	320, 1253
HpyF3I	CTNAG	4 cut(s)	207, 504, 660, 786
HpySE526I	ACGT	2 cut(s)	1038, 1071
Hsp92II	CATG	8 cut(s)	69, 170, 302, 410, 461, 584, 1247, 1255
KspAI	GTTAAC	1 cut(s)	1210
Kzo9I	GATC	4 cut(s)	33, 709, 772, 1094
LguI	GCTCTTC	2 cut(s)	459, 696
LmnI	GCTCC	1 cut(s)	307
Lsp1109I	GCAGC	4 cut(s)	424, 698, 709, 734
LweI	GCATC	4 cut(s)	322, 381, 414, 1068
MaeI	CTAG	1 cut(s)	488
MaeII	ACGT	2 cut(s)	1038, 1071
MaeIII	GTNAC	5 cut(s)	265, 583, 793, 847, 1301
MalI	GATC	4 cut(s)	35, 711, 774, 1096
MboI	GATC	4 cut(s)	33, 709, 772, 1094
MfeI	CAATTG	1 cut(s)	593
MflI	RGATCY	2 cut(s)	709, 772
MhlI	GDGCHC	3 cut(s)	479, 617, 1187
MlsI	TGGCCA	1 cut(s)	1256
MluCI	AATT	6 cut(s)	443, 593, 806, 909, 1066, 1217
MluNI	TGGCCA	1 cut(s)	1256
MlyI	GAGTC	3 cut(s)	49, 776, 836
MmeI	TCCRAC	7 cut(s)	77, 127, 207, 268, 520, 531, 1129
Mox20I	TGGCCA	1 cut(s)	1256
MscI	TGGCCA	1 cut(s)	1256
MseI	TTAA	4 cut(s)	48, 317, 1088, 1209
MslI	CAYNNNNRTG	2 cut(s)	399, 422
Msp20I	TGGCCA	1 cut(s)	1256
MspA1I	CMGCKG	1 cut(s)	503
MspI	CCGG	1 cut(s)	1130
MspR9I	CCNGG	3 cut(s)	384, 1130, 1131
MunI	CAATTG	1 cut(s)	593
Mva1269I	GAATGC	1 cut(s)	1158
MvaI	CCWGG	1 cut(s)	384
MwoI	GCNNNNNNNGC	2 cut(s)	320, 1253
NciI	CCSGG	2 cut(s)	1130, 1131
NcoI	CCATGG	1 cut(s)	1251
NdeII	GATC	4 cut(s)	33, 709, 772, 1094
NlaIII	CATG	8 cut(s)	69, 170, 302, 410, 461, 584, 1247, 1255
NlaIV	GGNNCC	2 cut(s)	381, 627
NmuCI	GTSAC	1 cut(s)	583
NspI	RCATGY	1 cut(s)	584
OliI	CACNNNNGTG	1 cut(s)	399
PaeR7I	CTCGAG	1 cut(s)	616
PciI	ACATGT	1 cut(s)	580
PciSI	GCTCTTC	2 cut(s)	459, 696
PcsI	WCGNNNNNNNCGW	1 cut(s)	1044
PctI	GAATGC	1 cut(s)	1158
PfeI	GAWTC	4 cut(s)	150, 162, 172, 835
PkrI	GCNGC	4 cut(s)	439, 688, 699, 724
PleI	GAGTC	3 cut(s)	49, 776, 836
PpsI	GAGTC	3 cut(s)	49, 776, 836
PpuMI	RGGWCCY	1 cut(s)	626
PscI	ACATGT	1 cut(s)	580
PshBI	ATTAAT	1 cut(s)	1088
PsiI	TTATAA	1 cut(s)	249
Psp124BI	GAGCTC	1 cut(s)	617
Psp1406I	AACGTT	1 cut(s)	1038
Psp5II	RGGWCCY	1 cut(s)	626
Psp6I	CCWGG	1 cut(s)	382
PspGI	CCWGG	1 cut(s)	382
PspN4I	GGNNCC	2 cut(s)	381, 627
PspPI	GGNCC	1 cut(s)	626
PspPPI	RGGWCCY	1 cut(s)	626
PstNI	CAGNNNCTG	1 cut(s)	263
PsuI	RGATCY	2 cut(s)	709, 772
PvuII	CAGCTG	1 cut(s)	503
RsaI	GTAC	3 cut(s)	254, 862, 1199
RsaNI	GTAC	3 cut(s)	253, 861, 1198
RseI	CAYNNNNRTG	2 cut(s)	399, 422
SacI	GAGCTC	1 cut(s)	617
SapI	GCTCTTC	2 cut(s)	459, 696
SaqAI	TTAA	4 cut(s)	48, 317, 1088, 1209
SatI	GCNGC	4 cut(s)	438, 687, 698, 723
Sau3AI	GATC	4 cut(s)	33, 709, 772, 1094
Sau96I	GGNCC	1 cut(s)	626
SchI	GAGTC	3 cut(s)	49, 776, 836
ScrFI	CCNGG	3 cut(s)	384, 1130, 1131
SduI	GDGCHC	3 cut(s)	479, 617, 1187
SfaNI	GCATC	4 cut(s)	322, 381, 414, 1068
SfcI	CTRYAG	1 cut(s)	470
Sfr274I	CTCGAG	1 cut(s)	616
SinI	GGWCC	1 cut(s)	626
SlaI	CTCGAG	1 cut(s)	616
SmaI	CCCGGG	1 cut(s)	1131
SmiMI	CAYNNNNRTG	2 cut(s)	399, 422
SmlI	CTYRAG	2 cut(s)	616, 899
SmoI	CTYRAG	2 cut(s)	616, 899
Sse9I	AATT	6 cut(s)	443, 593, 806, 909, 1066, 1217
SsiI	CCGC	3 cut(s)	669, 695, 816
SspMI	CTAG	1 cut(s)	488
SstI	GAGCTC	1 cut(s)	617
StyD4I	CCNGG	3 cut(s)	382, 1128, 1129
StyI	CCWWGG	1 cut(s)	1251
TaaI	ACNGT	4 cut(s)	481, 799, 961, 1003
TaiI	ACGT	2 cut(s)	1041, 1074
TaqI	TCGA	3 cut(s)	135, 617, 1074
TasI	AATT	6 cut(s)	443, 593, 806, 909, 1066, 1217
TfiI	GAWTC	4 cut(s)	150, 162, 172, 835
Tru1I	TTAA	4 cut(s)	48, 317, 1088, 1209
Tru9I	TTAA	4 cut(s)	48, 317, 1088, 1209
TscAI	CASTG	2 cut(s)	486, 966
TseFI	GTSAC	1 cut(s)	583
TseI	GCWGC	4 cut(s)	437, 686, 697, 722
Tsp45I	GTSAC	1 cut(s)	583
TspDTI	ATGAA	4 cut(s)	54, 474, 958, 1232
TspMI	CCCGGG	1 cut(s)	1129
TspRI	CASTG	2 cut(s)	486, 966
VpaK11BI	GGWCC	1 cut(s)	626
VspI	ATTAAT	1 cut(s)	1088
XapI	RAATTY	1 cut(s)	806
XceI	RCATGY	1 cut(s)	584
XhoI	CTCGAG	1 cut(s)	616
XmaI	CCCGGG	1 cut(s)	1129
XspI	CTAG	1 cut(s)	488

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.