Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005263

Physical Location & Seq

Genomic Coordinates Forward (+)

20491064 .. 20494924

Gene Structure 3861 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1178700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGAGTTCTGCATGCAACTCTCTCTGGTTTTAGTTCTTTCTGTGTTTGTTGTTCCAGCAGCTGTGCTGTCAGATTCCTCTCTGAGCTCAAAAGGGGTCTCAGAAATCCCCAAGAAGCTGCTCAATCTAGCCCAGAAACCCCAGGTGTTTGATTGGATGGTGGGAATCAGGAGGAAAATACATGAAAACCCAGAACTGGGTTATGAGGAATTTGAGACCAGTGAGCTGATCAGAGCAGAATTGGATAAAATGGGTATTGAATATAAACACCCAATTGGGGTTACTGGTGTTGTAGGCTTCATTGGCACCCGAAAACCGCCTTTCGTCGCAATCAGAGCTGATATGGATGCTCTGGCTATGCAGGAAATGGTGGAGTGGGAGCACAAGAGTAAAAATGCAGGGAAAATGCATGCTTGCGGGCACGATGCTCATGTTGCAATGCTTCTTGGTGCTGCAAAGATCCTTAAAGACCATGAGAAAGATTTGCAGGGAACAGTTGTTCTTGTATTTCAACCAGCGGAGGAAGGAGGAGCAGGGGCTAAGAAAATGATAGCTGGTGGAGCGTTAGAGAATGTTAGTGCTATTTTTGGGTTGCATGTTGCTAACCACATACCTATTGCTGAAGTGGCCTCCAGACCTGGTCCTTTTTTTGCTGGGAGTGGCTTCTTTGAAGCGACAATAAGTGGAAAAGGCGGTCATGCAGCCCTTCCTCACCATGCTGTTGACCCGATACTGGCAGCTTCTAATGTGATTGTCAGCTTGCAACATCTTGTTTCGCGTGAAGCTGATCCACTTGACTCACAGGTAGTCACTGTTGGAAAGTTTCAAGGAGGTGACGCATTCAACGTTATTCCAGATTCTGTCAAAATAGGTGGTACTTTCCGTGCATTTACAATGAAAAGCCTAATGCAACTCAAGCAGCGGATTAAGCAGGTTATAACAGGACAAGCATCCGTACATAGGTGCATCGCAACTGTCAATTTCTTTGAAGATGAGAAACCTATTTCCCCCCCAACCATAAACCATAACGACTTGCACGAACACTTCCGAAATGTAGCTGGAGATATGCTTGGCTTCCAAAAGGTTAAAGACCATCAACCGTTGATGGGATCCGAGGATTTTGCATATTACCAAGAGGCAATACCTGGATACTTTTTCTTGCTTGGAATGGCAGATGACGCACTTGGACCCCTGCCTACGCCTCATTCACCTCACTTCCGAATCAATGAAGATGCACTTCCTTTTGGTGCTGCGCTCCATGCTGCTTTAGCTGTTAGGTATTTGCTCGAGTTTCCACAGGAAGTTCCTTCGCCAGCGCAAGAATACCACGATGAATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006950 GO:0008150 GO:0009611 GO:0010112 GO:0010178 GO:0010179 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0031347 GO:0032101 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0071944 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

447

Amino Acids

48.55

Weight (kDa)

5.85

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	110 - 424	7.2e-43	Peptidase family M20/M25/M40
M20_dimer	PF07687	219 - 314	1.2e-12	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005263)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51760 AT1G51780
fragaria_vesca	FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830
malus_domestica	MD09G1197800.v1.1 MD17G1178700.v1.1
prunus_persica	Prupe.3G053700_v2.0.a1
pyrus_communis	pycom17g18780
rosa_chinensis	RchiOBHm_Chr2g0142041
rosa_laevigata	RLG00000019947
rosa_multiflora	Rmu_sc0004757.1_g000007
rosa_roxburghii	Rroxscaffold_2G00103650
rosa_rugosa	Rorug02G0369800 Rorug02G0369900 Rorug02G0370000
rosa_samantha	Rh2AG422500 Rh2BG432700 Rh2CG409400 Rh2DG442400
rosa_wichuraiana	Rw2G034620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	938, 1339
Acc36I	ACCTGC	1 cut(s)	922
AccB1I	GGYRCC	1 cut(s)	305
AccII	CGCG	1 cut(s)	778
AciI	CCGC	5 cut(s)	317, 417, 518, 693, 922
AclI	AACGTT	1 cut(s)	846
AclWI	GGATC	4 cut(s)	454, 782, 1104, 1117
AcsI	RAATTY	1 cut(s)	209
AcuI	CTGAAG	1 cut(s)	642
AfaI	GTAC	2 cut(s)	877, 957
AfiI	CCNNNNNNNGG	6 cut(s)	196, 197, 277, 733, 960, 1106
AgsI	TTSAA	6 cut(s)	260, 512, 671, 827, 844, 989
AjnI	CCWGG	3 cut(s)	141, 637, 1144
AloI	GAACNNNNNNTCC	2 cut(s)	483, 515
Alw21I	GWGCWC	2 cut(s)	89, 384
Alw26I	GTCTC	2 cut(s)	103, 209
AlwI	GGATC	4 cut(s)	454, 782, 1104, 1117
AlwNI	CAGNNNCTG	2 cut(s)	62, 860
Ama87I	CYCGRG	1 cut(s)	1286
AoxI	GGCC	1 cut(s)	627
ApeKI	GCWGC	8 cut(s)	59, 118, 452, 701, 737, 919, 1250, 1262
ApoI	RAATTY	1 cut(s)	209
AspLEI	GCGC	2 cut(s)	1255, 1318
AspS9I	GGNCC	2 cut(s)	641, 1187
AsuHPI	GGTGA	3 cut(s)	704, 845, 1200
AvaI	CYCGRG	1 cut(s)	1286
AvaII	GGWCC	2 cut(s)	641, 1187
BaeGI	GKGCMC	1 cut(s)	423
BamHI	GGATCC	1 cut(s)	1109
BanI	GGYRCC	1 cut(s)	305
BanII	GRGCYC	1 cut(s)	89
Bbv12I	GWGCWC	2 cut(s)	89, 384
BbvI	GCAGC	8 cut(s)	71, 105, 439, 713, 749, 931, 1237, 1249
BccI	CCATC	3 cut(s)	151, 1099, 1101
BcgI	CGANNNNNNTGC	2 cut(s)	1103, 1137
BciT130I	CCWGG	3 cut(s)	143, 639, 1146
BciVI	GTATCC	1 cut(s)	1142
BclI	TGATCA	1 cut(s)	228
BcoDI	GTCTC	2 cut(s)	103, 209
BfaI	CTAG	1 cut(s)	128
BfuAI	ACCTGC	1 cut(s)	922
BfuI	GTATCC	1 cut(s)	1142
BisI	GCNGC	8 cut(s)	60, 119, 453, 702, 738, 920, 1251, 1263
BlsI	GCNGC	8 cut(s)	61, 120, 454, 703, 739, 921, 1252, 1264
Bme1390I	CCNGG	3 cut(s)	143, 639, 1146
Bme18I	GGWCC	2 cut(s)	641, 1187
BmeT110I	CYCGRG	1 cut(s)	1286
BmgT120I	GGNCC	2 cut(s)	641, 1187
BmiI	GGNNCC	3 cut(s)	307, 1111, 1189
BmrFI	CCNGG	3 cut(s)	143, 639, 1146
BmrI	ACTGGG	1 cut(s)	206
BmsI	GCATC	5 cut(s)	337, 415, 959, 975, 1222
BmuI	ACTGGG	1 cut(s)	206
BpmI	CTGGAG	2 cut(s)	616, 1080
BpuEI	CTTGAG	1 cut(s)	899
BsaI	GGTCTC	2 cut(s)	103, 209
BsaJI	CCNNGG	2 cut(s)	141, 1113
Bsc4I	CCNNNNNNNGG	6 cut(s)	196, 197, 277, 733, 960, 1106
Bse1I	ACTGG	4 cut(s)	201, 219, 289, 738
Bse3DI	GCAATG	1 cut(s)	444
BseBI	CCWGG	3 cut(s)	143, 639, 1146
BseDI	CCNNGG	2 cut(s)	141, 1113
BseGI	GGATG	3 cut(s)	162, 352, 950
BseLI	CCNNNNNNNGG	6 cut(s)	196, 197, 277, 733, 960, 1106
BseMI	GCAATG	1 cut(s)	444
BseMII	CTCAG	2 cut(s)	74, 114
BseNI	ACTGG	4 cut(s)	201, 219, 289, 738
BseRI	GAGGAG	1 cut(s)	543
BseSI	GKGCMC	1 cut(s)	423
BseXI	GCAGC	8 cut(s)	71, 105, 439, 713, 749, 931, 1237, 1249
BseYI	CCCAGC	1 cut(s)	653
Bsh1236I	CGCG	1 cut(s)	778
BshFI	GGCC	1 cut(s)	629
BshNI	GGYRCC	1 cut(s)	305
BsiHKAI	GWGCWC	2 cut(s)	89, 384
BsiHKCI	CYCGRG	1 cut(s)	1286
BslI	CCNNNNNNNGG	6 cut(s)	196, 197, 277, 733, 960, 1106
BsmAI	GTCTC	2 cut(s)	103, 209
BsmI	GAATGC	1 cut(s)	839
BsnI	GGCC	1 cut(s)	629
Bso31I	GGTCTC	2 cut(s)	103, 209
BsoBI	CYCGRG	1 cut(s)	1286
Bsp1286I	GDGCHC	3 cut(s)	89, 384, 423
Bsp143I	GATC	4 cut(s)	228, 459, 787, 1109
BspACI	CCGC	5 cut(s)	317, 417, 518, 693, 922
BspANI	GGCC	1 cut(s)	629
BspCNI	CTCAG	2 cut(s)	75, 113
BspFNI	CGCG	1 cut(s)	778
BspLI	GGNNCC	3 cut(s)	307, 1111, 1189
BspMI	ACCTGC	1 cut(s)	922
BspPI	GGATC	4 cut(s)	454, 782, 1104, 1117
BspT107I	GGYRCC	1 cut(s)	305
BspTNI	GGTCTC	2 cut(s)	103, 209
BsrDI	GCAATG	1 cut(s)	444
BsrI	ACTGG	4 cut(s)	201, 219, 289, 738
BssECI	CCNNGG	2 cut(s)	141, 1113
BssMI	GATC	4 cut(s)	228, 459, 787, 1109
Bst2UI	CCWGG	3 cut(s)	143, 639, 1146
Bst4CI	ACNGT	4 cut(s)	496, 814, 976, 1101
BstC8I	GCNNGC	6 cut(s)	14, 411, 415, 419, 761, 1314
BstDEI	CTNAG	3 cut(s)	83, 100, 540
BstF5I	GGATG	3 cut(s)	162, 352, 950
BstFNI	CGCG	1 cut(s)	778
BstHHI	GCGC	2 cut(s)	1255, 1318
BstKTI	GATC	4 cut(s)	231, 462, 790, 1112
BstMAI	GTCTC	2 cut(s)	103, 209
BstMBI	GATC	4 cut(s)	228, 459, 787, 1109
BstNI	CCWGG	3 cut(s)	143, 639, 1146
BstNSI	RCATGY	3 cut(s)	16, 413, 599
BstSCI	CCNGG	3 cut(s)	141, 637, 1144
BstSLI	GKGCMC	1 cut(s)	423
BstUI	CGCG	1 cut(s)	778
BstV1I	GCAGC	8 cut(s)	71, 105, 439, 713, 749, 931, 1237, 1249
BstX2I	RGATCY	2 cut(s)	459, 1109
BstYI	RGATCY	2 cut(s)	459, 1109
BsuI	GTATCC	1 cut(s)	1142
BsuRI	GGCC	1 cut(s)	629
BtgZI	GCGATG	1 cut(s)	952
BtsCI	GGATG	3 cut(s)	162, 352, 950
BtsIMutI	CAGTG	2 cut(s)	226, 810
BveI	ACCTGC	1 cut(s)	922
Cac8I	GCNNGC	6 cut(s)	14, 411, 415, 419, 761, 1314
CaiI	CAGNNNCTG	2 cut(s)	62, 860
CfoI	GCGC	2 cut(s)	1255, 1318
Cfr13I	GGNCC	2 cut(s)	641, 1187
CseI	GACGC	2 cut(s)	845, 1187
CsiI	ACCWGGT	1 cut(s)	637
Csp6I	GTAC	2 cut(s)	876, 956
CspCI	CAANNNNNGTGG	2 cut(s)	853, 888
CviAII	CATG	9 cut(s)	13, 182, 410, 431, 473, 596, 698, 716, 1259
CviQI	GTAC	2 cut(s)	876, 956
DdeI	CTNAG	3 cut(s)	83, 100, 540
DpnI	GATC	4 cut(s)	230, 461, 789, 1111
DpnII	GATC	4 cut(s)	228, 459, 787, 1109
Ecl136II	GAGCTC	1 cut(s)	87
Eco24I	GRGCYC	1 cut(s)	89
Eco31I	GGTCTC	2 cut(s)	103, 209
Eco47I	GGWCC	2 cut(s)	641, 1187
Eco53kI	GAGCTC	1 cut(s)	87
Eco57I	CTGAAG	1 cut(s)	642
Eco88I	CYCGRG	1 cut(s)	1286
EcoICRI	GAGCTC	1 cut(s)	87
EcoRII	CCWGG	3 cut(s)	141, 637, 1144
EcoT22I	ATGCAT	1 cut(s)	411
EcoT38I	GRGCYC	1 cut(s)	89
FaeI	CATG	9 cut(s)	16, 185, 413, 434, 476, 599, 701, 719, 1262
FalI	AAGNNNNNCTT	2 cut(s)	1221, 1253
FatI	CATG	9 cut(s)	12, 181, 409, 430, 472, 595, 697, 715, 1258
FauI	CCCGC	1 cut(s)	410
FbaI	TGATCA	1 cut(s)	228
Fnu4HI	GCNGC	8 cut(s)	60, 119, 453, 702, 738, 920, 1251, 1263
FokI	GGATG	3 cut(s)	169, 359, 937
FriOI	GRGCYC	1 cut(s)	89
Fsp4HI	GCNGC	8 cut(s)	60, 119, 453, 702, 738, 920, 1251, 1263
FspBI	CTAG	1 cut(s)	128
GlaI	GCGC	2 cut(s)	1254, 1317
GluI	GCNGC	8 cut(s)	60, 119, 453, 702, 738, 920, 1251, 1263
GsaI	CCCAGC	1 cut(s)	657
GsuI	CTGGAG	2 cut(s)	616, 1080
HaeIII	GGCC	1 cut(s)	629
HgaI	GACGC	2 cut(s)	845, 1187
HhaI	GCGC	2 cut(s)	1255, 1318
Hin1II	CATG	9 cut(s)	16, 185, 413, 434, 476, 599, 701, 719, 1262
Hin6I	GCGC	2 cut(s)	1253, 1316
HinP1I	GCGC	2 cut(s)	1253, 1316
HincII	GTYRAC	1 cut(s)	724
HindII	GTYRAC	1 cut(s)	724
HinfI	GANTC	5 cut(s)	74, 165, 797, 857, 1221
HphI	GGTGA	3 cut(s)	704, 845, 1200
Hpy166II	GTNNAC	1 cut(s)	724
Hpy188I	TCNGA	8 cut(s)	73, 84, 103, 233, 335, 1049, 1114, 1220
Hpy188III	TCNNGA	3 cut(s)	169, 633, 854
Hpy8I	GTNNAC	1 cut(s)	724
Hpy99I	CGWCG	1 cut(s)	329
HpyAV	CCTTC	3 cut(s)	518, 716, 1317
HpyCH4III	ACNGT	4 cut(s)	496, 814, 976, 1101
HpyCH4IV	ACGT	1 cut(s)	846
HpyF3I	CTNAG	3 cut(s)	83, 100, 540
HpySE526I	ACGT	1 cut(s)	846
Hsp92II	CATG	9 cut(s)	16, 185, 413, 434, 476, 599, 701, 719, 1262
HspAI	GCGC	2 cut(s)	1253, 1316
Ksp22I	TGATCA	1 cut(s)	228
Kzo9I	GATC	4 cut(s)	228, 459, 787, 1109
LmnI	GCTCC	4 cut(s)	379, 530, 560, 1260
Lsp1109I	GCAGC	8 cut(s)	71, 105, 439, 713, 749, 931, 1237, 1249
LweI	GCATC	5 cut(s)	337, 415, 959, 975, 1222
MabI	ACCWGGT	1 cut(s)	637
MaeI	CTAG	1 cut(s)	128
MaeII	ACGT	1 cut(s)	846
MaeIII	GTNAC	3 cut(s)	280, 808, 833
MalI	GATC	4 cut(s)	230, 461, 789, 1111
MboI	GATC	4 cut(s)	228, 459, 787, 1109
MboII	GAAGA	2 cut(s)	1001, 1241
MfeI	CAATTG	1 cut(s)	273
MflI	RGATCY	2 cut(s)	459, 1109
MhlI	GDGCHC	3 cut(s)	89, 384, 423
MluCI	AATT	5 cut(s)	209, 239, 273, 979, 1334
MlyI	GAGTC	1 cut(s)	791
MmeI	TCCRAC	1 cut(s)	796
Mph1103I	ATGCAT	1 cut(s)	411
MseI	TTAA	3 cut(s)	465, 927, 1086
MspA1I	CMGCKG	3 cut(s)	62, 518, 922
MspR9I	CCNGG	3 cut(s)	143, 639, 1146
MunI	CAATTG	1 cut(s)	273
Mva1269I	GAATGC	1 cut(s)	839
MvaI	CCWGG	3 cut(s)	143, 639, 1146
MvnI	CGCG	1 cut(s)	778
NdeII	GATC	4 cut(s)	228, 459, 787, 1109
NlaIII	CATG	9 cut(s)	16, 185, 413, 434, 476, 599, 701, 719, 1262
NlaIV	GGNNCC	3 cut(s)	307, 1111, 1189
NmuCI	GTSAC	2 cut(s)	808, 833
NsiI	ATGCAT	1 cut(s)	411
NspI	RCATGY	3 cut(s)	16, 413, 599
PaeI	GCATGC	2 cut(s)	16, 413
PaeR7I	CTCGAG	1 cut(s)	1286
PcsI	WCGNNNNNNNCGW	2 cut(s)	843, 1035
PctI	GAATGC	1 cut(s)	839
PfeI	GAWTC	4 cut(s)	74, 165, 857, 1221
PflFI	GACNNNGTC	1 cut(s)	639
PkrI	GCNGC	8 cut(s)	61, 120, 454, 703, 739, 921, 1252, 1264
PleI	GAGTC	1 cut(s)	791
PpsI	GAGTC	1 cut(s)	791
PsiI	TTATAA	2 cut(s)	938, 1339
Psp124BI	GAGCTC	1 cut(s)	89
Psp1406I	AACGTT	1 cut(s)	846
Psp6I	CCWGG	3 cut(s)	141, 637, 1144
PspFI	CCCAGC	1 cut(s)	653
PspGI	CCWGG	3 cut(s)	141, 637, 1144
PspN4I	GGNNCC	3 cut(s)	307, 1111, 1189
PspPI	GGNCC	2 cut(s)	641, 1187
PspXI	VCTCGAGB	1 cut(s)	1286
PstNI	CAGNNNCTG	2 cut(s)	62, 860
PsuI	RGATCY	2 cut(s)	459, 1109
PsyI	GACNNNGTC	1 cut(s)	639
PvuII	CAGCTG	1 cut(s)	62
RsaI	GTAC	2 cut(s)	877, 957
RsaNI	GTAC	2 cut(s)	876, 956
SacI	GAGCTC	1 cut(s)	89
SaqAI	TTAA	3 cut(s)	465, 927, 1086
SatI	GCNGC	8 cut(s)	60, 119, 453, 702, 738, 920, 1251, 1263
Sau3AI	GATC	4 cut(s)	228, 459, 787, 1109
Sau96I	GGNCC	2 cut(s)	641, 1187
SchI	GAGTC	1 cut(s)	791
ScrFI	CCNGG	3 cut(s)	143, 639, 1146
SduI	GDGCHC	3 cut(s)	89, 384, 423
SexAI	ACCWGGT	1 cut(s)	637
SfaNI	GCATC	5 cut(s)	337, 415, 959, 975, 1222
Sfr274I	CTCGAG	1 cut(s)	1286
SinI	GGWCC	2 cut(s)	641, 1187
SlaI	CTCGAG	1 cut(s)	1286
SmlI	CTYRAG	2 cut(s)	914, 1286
SmoI	CTYRAG	2 cut(s)	914, 1286
SphI	GCATGC	2 cut(s)	16, 413
Sse9I	AATT	5 cut(s)	209, 239, 273, 979, 1334
SsiI	CCGC	5 cut(s)	317, 417, 518, 693, 922
SspMI	CTAG	1 cut(s)	128
SstI	GAGCTC	1 cut(s)	89
StyD4I	CCNGG	3 cut(s)	141, 637, 1144
TaaI	ACNGT	4 cut(s)	496, 814, 976, 1101
TaiI	ACGT	1 cut(s)	849
TaqI	TCGA	1 cut(s)	1287
TasI	AATT	5 cut(s)	209, 239, 273, 979, 1334
TfiI	GAWTC	4 cut(s)	74, 165, 857, 1221
Tru1I	TTAA	3 cut(s)	465, 927, 1086
Tru9I	TTAA	3 cut(s)	465, 927, 1086
TscAI	CASTG	2 cut(s)	226, 817
TseFI	GTSAC	2 cut(s)	808, 833
TseI	GCWGC	8 cut(s)	59, 118, 452, 701, 737, 919, 1250, 1262
Tsp45I	GTSAC	2 cut(s)	808, 833
TspDTI	ATGAA	4 cut(s)	198, 289, 911, 1242
TspGWI	ACGGA	2 cut(s)	872, 943
TspRI	CASTG	2 cut(s)	226, 817
Tth111I	GACNNNGTC	1 cut(s)	639
VpaK11BI	GGWCC	2 cut(s)	641, 1187
XapI	RAATTY	1 cut(s)	209
XceI	RCATGY	3 cut(s)	16, 413, 599
XhoI	CTCGAG	1 cut(s)	1286
XspI	CTAG	1 cut(s)	128
Zsp2I	ATGCAT	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.