Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005263

Physical Location & Seq

Genomic Coordinates Forward (+)

17986294 .. 17988997

Gene Structure 2704 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1197800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGAAGTTATGCATGAAACTCTCTCTGGTTTTAGTTCTTTCTGTGTTTGTTGTTCCAGCAACTGTGCTATCAGATTCCCCCCTGAGCTCAAATGGGGTCTCAGAAATCCCTAAGAAGTTGCTCAGTTTTGCCCAGAACCCCCAGGTGTTTGATTGGATGGTGGGAATCAGGAGGAAAATACATGCAAACCCAGAACTGGGTTATGAGGAATTTGAGACCAGTGAGCTGATCAGAGCAGAATTGGATCAAATGAGTATTGAATATAAACATCCAGTTGGGGTTACTGGTGTTGTAGGCTTCATTGGCACCCAAAAACCGCCTTTCGTTGCAATCAGAGCTGATATGGATGCTCTGACCATGCAGGAAATGCTGGAGTGGGTGCACAAGAGTAAAAATGCAGGGAAAATGCATGCTTGTGGGCATGATGCTCACGTTGCAATGCTTCTTGGTGCAGCAAAGATCCTTAAAGAGCATGAGCAAGATTTGCAGGGAACAGTTGTTCTTGTATTTCAACCGGCGGAGGAAGGAGGAGCAGGGGCTAAGAAAATGATAGCTGGTGGAGCATTAGAGAATGTTAGTGCTATTTTTGGGTTGCATGTTGCAAACCACGTACCTATTGCTGAAGTGGCCTCCAGACCTGGTCCCTTTTTAGCTGGGGCTGGCTTCTTTGAAGCGACAATAAGTGGAAAAGGTGGTCATGCAGCCCTTCCTCACCACGCAGTTGACCCGATCCTGGCAGCTTCTAATGTGATTGTCAGCTTGCAACATCTTGTCTCGCGTGAAGCTGATCCACTCGACTCGCAGGTAATCACTGTTGGAAAATTTCAAGGAGGTGATGCATTCAATGTTATTCCAGATTCTGTCAAAATAGATGGAACTTTCCGTGCATTTACAACGGAAAGCCTAATGCAACTCAAGCAGCGGATTAAGCAGGTTATAACAGGACAAGCATCGGTACACAGGTGCCTCGCAACTGTCAATTTCTTTGAAGATGAGAAACCTTTGTCCCCACCGACCGTAAACCATAAGGACTTGCGCGAACACTTCAGAAACGTAGCTGGAGATATGCTTGGCTTCGAAAAGGTCAAGGACCATCAACGGTGGATGGGATCCGAGGATTTTGCATACTACCAAGAGGCAATACCTGGATACTTTTCCTTCCTTGGAATGGCAGATGATGCACTTGGACCCCTGCCTATGCCGCATTCACCCTACTTCCGTATCAACGAAGATGCACTTCCTTATGGTGCTGCACTCCATGCTTCTTTCGCTGCGAGGTATTTGCTCGAGTTTCAACAGGAAGTTCCTTTGCCAGAACGAGAACACCATGATGAATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0006950 GO:0008150 GO:0009611 GO:0010112 GO:0010178 GO:0010179 GO:0012505 GO:0016020 GO:0016787 GO:0016810 GO:0031347 GO:0032101 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0071944 GO:0080134

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

447

Amino Acids

48.81

Weight (kDa)

5.75

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	110 - 425	3.4e-41	Peptidase family M20/M25/M40
M20_dimer	PF07687	219 - 314	4.3e-14	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005263)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51760 AT1G51780
fragaria_vesca	FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830 FvH4_6g32830
malus_domestica	MD09G1197800.v1.1 MD17G1178700.v1.1
prunus_persica	Prupe.3G053700_v2.0.a1
pyrus_communis	pycom17g18780
rosa_chinensis	RchiOBHm_Chr2g0142041
rosa_laevigata	RLG00000019947
rosa_multiflora	Rmu_sc0004757.1_g000007
rosa_roxburghii	Rroxscaffold_2G00103650
rosa_rugosa	Rorug02G0369800 Rorug02G0369900 Rorug02G0370000
rosa_samantha	Rh2AG422500 Rh2BG432700 Rh2CG409400 Rh2DG442400
rosa_wichuraiana	Rw2G034620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	938, 1339
Acc36I	ACCTGC	2 cut(s)	793, 922
AccB1I	GGYRCC	2 cut(s)	305, 963
AccII	CGCG	2 cut(s)	778, 1038
AciI	CCGC	4 cut(s)	317, 518, 922, 1202
AclWI	GGATC	6 cut(s)	252, 454, 724, 782, 1104, 1117
AcsI	RAATTY	2 cut(s)	209, 821
AcuI	CTGAAG	2 cut(s)	642, 1030
AfaI	GTAC	2 cut(s)	612, 957
AfiI	CCNNNNNNNGG	4 cut(s)	196, 197, 733, 1168
AgsI	TTSAA	7 cut(s)	260, 512, 671, 827, 844, 989, 1295
AjnI	CCWGG	4 cut(s)	141, 637, 732, 1144
AloI	GAACNNNNNNTCC	2 cut(s)	483, 515
AluBI	AGCT	9 cut(s)	87, 226, 338, 554, 653, 740, 759, 785, 1058
AluI	AGCT	9 cut(s)	87, 226, 338, 554, 653, 740, 759, 785, 1058
Alw21I	GWGCWC	2 cut(s)	89, 384
Alw26I	GTCTC	3 cut(s)	103, 209, 778
Alw44I	GTGCAC	1 cut(s)	380
AlwI	GGATC	6 cut(s)	252, 454, 724, 782, 1104, 1117
AlwNI	CAGNNNCTG	2 cut(s)	62, 860
Ama87I	CYCGRG	1 cut(s)	1286
AoxI	GGCC	1 cut(s)	627
ApaLI	GTGCAC	1 cut(s)	380
ApeKI	GCWGC	6 cut(s)	452, 701, 737, 919, 1250, 1271
ApoI	RAATTY	2 cut(s)	209, 821
AspLEI	GCGC	1 cut(s)	1038
AspS9I	GGNCC	3 cut(s)	641, 1090, 1187
AsuHPI	GGTGA	3 cut(s)	704, 845, 1200
AsuII	TTCGAA	1 cut(s)	1077
AvaI	CYCGRG	1 cut(s)	1286
AvaII	GGWCC	3 cut(s)	641, 1090, 1187
BaeGI	GKGCMC	1 cut(s)	384
BaeI	ACNNNNGTAYC	4 cut(s)	947, 980, 1204, 1237
BamHI	GGATCC	1 cut(s)	1109
BanI	GGYRCC	2 cut(s)	305, 963
BanII	GRGCYC	1 cut(s)	89
Bbv12I	GWGCWC	2 cut(s)	89, 384
BbvI	GCAGC	6 cut(s)	464, 713, 749, 931, 1237, 1258
BccI	CCATC	4 cut(s)	151, 866, 1099, 1101
BcgI	CGANNNNNNTGC	4 cut(s)	1103, 1137, 1264, 1298
BciT130I	CCWGG	4 cut(s)	143, 639, 734, 1146
BciVI	GTATCC	1 cut(s)	1142
BclI	TGATCA	1 cut(s)	228
BcoDI	GTCTC	3 cut(s)	103, 209, 778
BfuAI	ACCTGC	2 cut(s)	793, 922
BfuI	GTATCC	1 cut(s)	1142
BisI	GCNGC	7 cut(s)	453, 702, 738, 920, 1202, 1251, 1272
BlsI	GCNGC	7 cut(s)	454, 703, 739, 921, 1203, 1252, 1273
Bme1390I	CCNGG	4 cut(s)	143, 639, 734, 1146
Bme18I	GGWCC	3 cut(s)	641, 1090, 1187
BmeT110I	CYCGRG	1 cut(s)	1286
BmgT120I	GGNCC	3 cut(s)	641, 1090, 1187
BmiI	GGNNCC	5 cut(s)	307, 643, 965, 1111, 1189
BmrFI	CCNGG	4 cut(s)	143, 639, 734, 1146
BmrI	ACTGGG	1 cut(s)	206
BmsI	GCATC	6 cut(s)	337, 415, 826, 959, 1168, 1222
BmuI	ACTGGG	1 cut(s)	206
BpmI	CTGGAG	3 cut(s)	392, 616, 1080
Bpu10I	CCTNAGC	1 cut(s)	83
Bpu14I	TTCGAA	1 cut(s)	1077
BpuEI	CTTGAG	1 cut(s)	899
BsaAI	YACGTR	1 cut(s)	610
BsaI	GGTCTC	2 cut(s)	103, 209
BsaJI	CCNNGG	3 cut(s)	141, 1113, 1162
Bsc4I	CCNNNNNNNGG	4 cut(s)	196, 197, 733, 1168
Bse118I	RCCGGY	1 cut(s)	514
Bse1I	ACTGG	4 cut(s)	201, 219, 272, 289
Bse3DI	GCAATG	1 cut(s)	444
BseBI	CCWGG	4 cut(s)	143, 639, 734, 1146
BseDI	CCNNGG	3 cut(s)	141, 1113, 1162
BseGI	GGATG	4 cut(s)	162, 268, 352, 1110
BseLI	CCNNNNNNNGG	4 cut(s)	196, 197, 733, 1168
BseMI	GCAATG	1 cut(s)	444
BseMII	CTCAG	3 cut(s)	74, 114, 136
BseNI	ACTGG	4 cut(s)	201, 219, 272, 289
BseRI	GAGGAG	1 cut(s)	543
BseSI	GKGCMC	1 cut(s)	384
BseXI	GCAGC	6 cut(s)	464, 713, 749, 931, 1237, 1258
BseYI	CCCAGC	1 cut(s)	653
BsgI	GTGCAG	2 cut(s)	471, 1236
Bsh1236I	CGCG	2 cut(s)	778, 1038
Bsh1285I	CGRYCG	1 cut(s)	1017
BshFI	GGCC	1 cut(s)	629
BshNI	GGYRCC	2 cut(s)	305, 963
BsiEI	CGRYCG	1 cut(s)	1017
BsiHKAI	GWGCWC	2 cut(s)	89, 384
BsiHKCI	CYCGRG	1 cut(s)	1286
BsiSI	CCGG	1 cut(s)	515
BslFI	GGGAC	2 cut(s)	627, 991
BslI	CCNNNNNNNGG	4 cut(s)	196, 197, 733, 1168
BsmAI	GTCTC	3 cut(s)	103, 209, 778
BsmFI	GGGAC	2 cut(s)	627, 991
BsmI	GAATGC	2 cut(s)	839, 1204
BsnI	GGCC	1 cut(s)	629
Bso31I	GGTCTC	2 cut(s)	103, 209
BsoBI	CYCGRG	1 cut(s)	1286
Bsp119I	TTCGAA	1 cut(s)	1077
Bsp1286I	GDGCHC	2 cut(s)	89, 384
Bsp143I	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
BspACI	CCGC	4 cut(s)	317, 518, 922, 1202
BspANI	GGCC	1 cut(s)	629
BspCNI	CTCAG	3 cut(s)	75, 113, 135
BspFNI	CGCG	2 cut(s)	778, 1038
BspLI	GGNNCC	5 cut(s)	307, 643, 965, 1111, 1189
BspMI	ACCTGC	2 cut(s)	793, 922
BspPI	GGATC	6 cut(s)	252, 454, 724, 782, 1104, 1117
BspT104I	TTCGAA	1 cut(s)	1077
BspT107I	GGYRCC	2 cut(s)	305, 963
BspTNI	GGTCTC	2 cut(s)	103, 209
BsrDI	GCAATG	1 cut(s)	444
BsrFI	RCCGGY	1 cut(s)	514
BsrI	ACTGG	4 cut(s)	201, 219, 272, 289
BssAI	RCCGGY	1 cut(s)	514
BssECI	CCNNGG	3 cut(s)	141, 1113, 1162
BssMI	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
BssT1I	CCWWGG	1 cut(s)	1162
Bst2UI	CCWGG	4 cut(s)	143, 639, 734, 1146
Bst4CI	ACNGT	6 cut(s)	64, 496, 814, 976, 1018, 1101
BstAPI	GCANNNNNTGC	4 cut(s)	367, 484, 1178, 1259
BstBAI	YACGTR	1 cut(s)	610
BstBI	TTCGAA	1 cut(s)	1077
BstC8I	GCNNGC	3 cut(s)	411, 661, 761
BstDEI	CTNAG	5 cut(s)	83, 100, 111, 122, 540
BstF5I	GGATG	4 cut(s)	162, 268, 352, 1110
BstFNI	CGCG	2 cut(s)	778, 1038
BstHHI	GCGC	1 cut(s)	1038
BstKTI	GATC	6 cut(s)	231, 247, 462, 732, 790, 1112
BstMAI	GTCTC	3 cut(s)	103, 209, 778
BstMBI	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
BstMCI	CGRYCG	1 cut(s)	1017
BstNI	CCWGG	4 cut(s)	143, 639, 734, 1146
BstNSI	RCATGY	3 cut(s)	185, 413, 599
BstSCI	CCNGG	4 cut(s)	141, 637, 732, 1144
BstSLI	GKGCMC	1 cut(s)	384
BstUI	CGCG	2 cut(s)	778, 1038
BstV1I	GCAGC	6 cut(s)	464, 713, 749, 931, 1237, 1258
BstX2I	RGATCY	2 cut(s)	459, 1109
BstYI	RGATCY	2 cut(s)	459, 1109
BsuI	GTATCC	1 cut(s)	1142
BsuRI	GGCC	1 cut(s)	629
BtsCI	GGATG	4 cut(s)	162, 268, 352, 1110
BtsIMutI	CAGTG	2 cut(s)	226, 810
BveI	ACCTGC	2 cut(s)	793, 922
Cac8I	GCNNGC	3 cut(s)	411, 661, 761
CaiI	CAGNNNCTG	2 cut(s)	62, 860
CfoI	GCGC	1 cut(s)	1038
Cfr10I	RCCGGY	1 cut(s)	514
Cfr13I	GGNCC	3 cut(s)	641, 1090, 1187
CsiI	ACCWGGT	1 cut(s)	637
Csp6I	GTAC	2 cut(s)	611, 956
CviQI	GTAC	2 cut(s)	611, 956
DdeI	CTNAG	5 cut(s)	83, 100, 111, 122, 540
DpnI	GATC	6 cut(s)	230, 246, 461, 731, 789, 1111
DpnII	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
EciI	GGCGGA	1 cut(s)	533
Ecl136II	GAGCTC	1 cut(s)	87
Eco130I	CCWWGG	1 cut(s)	1162
Eco24I	GRGCYC	1 cut(s)	89
Eco31I	GGTCTC	2 cut(s)	103, 209
Eco47I	GGWCC	3 cut(s)	641, 1090, 1187
Eco53kI	GAGCTC	1 cut(s)	87
Eco57I	CTGAAG	2 cut(s)	642, 1030
Eco88I	CYCGRG	1 cut(s)	1286
EcoICRI	GAGCTC	1 cut(s)	87
EcoRII	CCWGG	4 cut(s)	141, 637, 732, 1144
EcoT14I	CCWWGG	1 cut(s)	1162
EcoT22I	ATGCAT	3 cut(s)	14, 411, 841
EcoT38I	GRGCYC	1 cut(s)	89
ErhI	CCWWGG	1 cut(s)	1162
FalI	AAGNNNNNCTT	2 cut(s)	1221, 1253
FaqI	GGGAC	2 cut(s)	627, 991
FbaI	TGATCA	1 cut(s)	228
Fnu4HI	GCNGC	7 cut(s)	453, 702, 738, 920, 1202, 1251, 1272
FokI	GGATG	4 cut(s)	169, 255, 359, 1117
FriOI	GRGCYC	1 cut(s)	89
Fsp4HI	GCNGC	7 cut(s)	453, 702, 738, 920, 1202, 1251, 1272
GlaI	GCGC	1 cut(s)	1037
GluI	GCNGC	7 cut(s)	453, 702, 738, 920, 1202, 1251, 1272
GsaI	CCCAGC	1 cut(s)	657
GsuI	CTGGAG	3 cut(s)	392, 616, 1080
HaeIII	GGCC	1 cut(s)	629
HapII	CCGG	1 cut(s)	515
HhaI	GCGC	1 cut(s)	1038
Hin6I	GCGC	1 cut(s)	1036
HinP1I	GCGC	1 cut(s)	1036
HincII	GTYRAC	1 cut(s)	724
HindII	GTYRAC	1 cut(s)	724
HinfI	GANTC	4 cut(s)	74, 165, 797, 857
HpaII	CCGG	1 cut(s)	515
HphI	GGTGA	3 cut(s)	704, 845, 1200
Hpy166II	GTNNAC	4 cut(s)	382, 724, 958, 1021
Hpy188I	TCNGA	7 cut(s)	73, 103, 233, 335, 354, 1049, 1114
Hpy188III	TCNNGA	3 cut(s)	169, 633, 854
Hpy8I	GTNNAC	4 cut(s)	382, 724, 958, 1021
HpyAV	CCTTC	3 cut(s)	518, 716, 1168
HpyCH4III	ACNGT	6 cut(s)	64, 496, 814, 976, 1018, 1101
HpyCH4IV	ACGT	3 cut(s)	432, 609, 1053
HpyF3I	CTNAG	5 cut(s)	83, 100, 111, 122, 540
HpySE526I	ACGT	3 cut(s)	432, 609, 1053
HspAI	GCGC	1 cut(s)	1036
Ksp22I	TGATCA	1 cut(s)	228
Kzo9I	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
LmnI	GCTCC	2 cut(s)	530, 560
Lsp1109I	GCAGC	6 cut(s)	464, 713, 749, 931, 1237, 1258
LweI	GCATC	6 cut(s)	337, 415, 826, 959, 1168, 1222
MabI	ACCWGGT	1 cut(s)	637
MaeII	ACGT	3 cut(s)	432, 609, 1053
MaeIII	GTNAC	1 cut(s)	280
MalI	GATC	6 cut(s)	230, 246, 461, 731, 789, 1111
MboI	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
MboII	GAAGA	2 cut(s)	1001, 1241
MflI	RGATCY	2 cut(s)	459, 1109
MhlI	GDGCHC	2 cut(s)	89, 384
MluCI	AATT	5 cut(s)	209, 239, 821, 979, 1334
MlyI	GAGTC	1 cut(s)	791
MmeI	TCCRAC	1 cut(s)	796
Mph1103I	ATGCAT	3 cut(s)	14, 411, 841
MseI	TTAA	2 cut(s)	465, 927
MslI	CAYNNNNRTG	2 cut(s)	414, 1329
MspA1I	CMGCKG	1 cut(s)	922
MspI	CCGG	1 cut(s)	515
MspR9I	CCNGG	4 cut(s)	143, 639, 734, 1146
Mva1269I	GAATGC	2 cut(s)	839, 1204
MvaI	CCWGG	4 cut(s)	143, 639, 734, 1146
MvnI	CGCG	2 cut(s)	778, 1038
NdeII	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
NlaIV	GGNNCC	5 cut(s)	307, 643, 965, 1111, 1189
NsiI	ATGCAT	3 cut(s)	14, 411, 841
NspI	RCATGY	3 cut(s)	185, 413, 599
NspV	TTCGAA	1 cut(s)	1077
PaeI	GCATGC	1 cut(s)	413
PaeR7I	CTCGAG	1 cut(s)	1286
PctI	GAATGC	2 cut(s)	839, 1204
PfeI	GAWTC	3 cut(s)	74, 165, 857
PflFI	GACNNNGTC	1 cut(s)	639
PkrI	GCNGC	7 cut(s)	454, 703, 739, 921, 1203, 1252, 1273
PleI	GAGTC	1 cut(s)	791
PpsI	GAGTC	1 cut(s)	791
Ppu21I	YACGTR	1 cut(s)	610
PsiI	TTATAA	2 cut(s)	938, 1339
Psp124BI	GAGCTC	1 cut(s)	89
Psp6I	CCWGG	4 cut(s)	141, 637, 732, 1144
PspFI	CCCAGC	1 cut(s)	653
PspGI	CCWGG	4 cut(s)	141, 637, 732, 1144
PspN4I	GGNNCC	5 cut(s)	307, 643, 965, 1111, 1189
PspPI	GGNCC	3 cut(s)	641, 1090, 1187
PspXI	VCTCGAGB	1 cut(s)	1286
PstNI	CAGNNNCTG	2 cut(s)	62, 860
PsuI	RGATCY	2 cut(s)	459, 1109
PsyI	GACNNNGTC	1 cut(s)	639
RsaI	GTAC	2 cut(s)	612, 957
RsaNI	GTAC	2 cut(s)	611, 956
RseI	CAYNNNNRTG	2 cut(s)	414, 1329
SacI	GAGCTC	1 cut(s)	89
SaqAI	TTAA	2 cut(s)	465, 927
SatI	GCNGC	7 cut(s)	453, 702, 738, 920, 1202, 1251, 1272
Sau3AI	GATC	6 cut(s)	228, 244, 459, 729, 787, 1109
Sau96I	GGNCC	3 cut(s)	641, 1090, 1187
SchI	GAGTC	1 cut(s)	791
ScrFI	CCNGG	4 cut(s)	143, 639, 734, 1146
SduI	GDGCHC	2 cut(s)	89, 384
SexAI	ACCWGGT	1 cut(s)	637
SfaNI	GCATC	6 cut(s)	337, 415, 826, 959, 1168, 1222
Sfr274I	CTCGAG	1 cut(s)	1286
SfuI	TTCGAA	1 cut(s)	1077
SinI	GGWCC	3 cut(s)	641, 1090, 1187
SlaI	CTCGAG	1 cut(s)	1286
SmiMI	CAYNNNNRTG	2 cut(s)	414, 1329
SmlI	CTYRAG	2 cut(s)	914, 1286
SmoI	CTYRAG	2 cut(s)	914, 1286
SphI	GCATGC	1 cut(s)	413
Sse9I	AATT	5 cut(s)	209, 239, 821, 979, 1334
SsiI	CCGC	4 cut(s)	317, 518, 922, 1202
SstI	GAGCTC	1 cut(s)	89
StyD4I	CCNGG	4 cut(s)	141, 637, 732, 1144
StyI	CCWWGG	1 cut(s)	1162
TaaI	ACNGT	6 cut(s)	64, 496, 814, 976, 1018, 1101
TaiI	ACGT	3 cut(s)	435, 612, 1056
TaqI	TCGA	3 cut(s)	795, 1077, 1287
TasI	AATT	5 cut(s)	209, 239, 821, 979, 1334
TauI	GCSGC	1 cut(s)	1204
TfiI	GAWTC	3 cut(s)	74, 165, 857
Tru1I	TTAA	2 cut(s)	465, 927
Tru9I	TTAA	2 cut(s)	465, 927
TscAI	CASTG	2 cut(s)	226, 817
TseI	GCWGC	6 cut(s)	452, 701, 737, 919, 1250, 1271
TspDTI	ATGAA	3 cut(s)	17, 29, 289
TspGWI	ACGGA	3 cut(s)	872, 911, 1208
TspRI	CASTG	2 cut(s)	226, 817
Tth111I	GACNNNGTC	1 cut(s)	639
VneI	GTGCAC	1 cut(s)	380
VpaK11BI	GGWCC	3 cut(s)	641, 1090, 1187
XapI	RAATTY	2 cut(s)	209, 821
XceI	RCATGY	3 cut(s)	185, 413, 599
XhoI	CTCGAG	1 cut(s)	1286
Zsp2I	ATGCAT	3 cut(s)	14, 411, 841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.