AT1G52500

Formamidopyrimidine-DNA glycosylase H2TH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005648
Physical Location & Seq
Forward (+)
19559880 .. 19563113
3234 bp
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UTR
Exon/CDS
Intron
AT1G52500.2

Sequence Viewer

Length: 825 bp
ATGCCGGAGCTTCCAGAGGTCGAGGCGGCGAGGAGAGCAATAGAAGAAAATTGTCTTGGAAAAAAGATTAAGCGAGTCATTATCGCCGACGACAACAAAGTGATCCATGGAATCTCGCCGTCCGATTTCCAGACCTCAATCCTCGGAAAAACCATCATCTCTGCTCGTAGAAAGGGCAAAAACCTCTGGCTCGAATTAGATTCCCCGCCATTTCCTTCTTTTCAATTCGGTATGGCTGGTGCTATTTATATCAAAGGCGTTGCAGTTACTAAGTATAAGAGGTCTGCTGTAAAGGACTCAGAGGAGTGGCCATCTAAGTATTCAAAGTTCTTCGTCGAGCTTGATGATGGTCTGGAGCTTTCATTTACTGATAAGAGGAGATTTGCCAAAGTTCGACTTCTAGCAAATCCTACTTCTGTGAGTCCAATATCAGAGCTTGGTCCTGATGCATTGTTGGAGCCTATGACAGTTGATGAATTTGCAGAAAGCTTAGCTAAAAAGAAAATAACCATAAAACCTCTCCTACTTGATCAGGGATATATTTCAGGTATTGGGAATTGGATCGCTGATGAAGTGCTGTACCAAGCCAGAATCCACCCGTTGCAGACTGCTTCTAGCCTCTCCAAAGAGCAATGTGAAGCTTTGCACACATCCATCAAAGAGGTCATTCAACATGCAGTTCAAGTCAATGCTGATAGCAAAGAGTTTCCTGTTGAATGGTTGTTTCATTTTCGGTGGGGGAAAAAAGCAGGGAAAGTTAATGGTAAGCTTTCCCACCATCTATCTATAAATTTGATGAAGCAGAATTTGGGGTTTTGCAGGTGA

Protein Analysis

274

Amino Acids

30.76

Weight (kDa)

9.07

Isoelectric Point (pI)

40.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 7.6e-30 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 236 1.8e-33 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 240 - 273 1.3e-24 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 810
Acc36I ACCTGC 1 cut(s) 810
AciI CCGC 2 cut(s) 26, 206
AclWI GGATC 2 cut(s) 97, 569
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 3 cut(s) 476, 790, 805
AfaI GTAC 1 cut(s) 581
AgsI TTSAA 5 cut(s) 224, 324, 671, 683, 716
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 791, 823
AluBI AGCT 8 cut(s) 10, 340, 358, 436, 489, 494, 641, 769
AluI AGCT 8 cut(s) 10, 340, 358, 436, 489, 494, 641, 769
AlwI GGATC 2 cut(s) 97, 569
AoxI GGCC 1 cut(s) 308
ApoI RAATTY 3 cut(s) 476, 790, 805
AspS9I GGNCC 1 cut(s) 440
AvaII GGWCC 1 cut(s) 440
BalI TGGCCA 1 cut(s) 310
BccI CCATC 5 cut(s) 161, 319, 341, 662, 786
BceAI ACGGC 1 cut(s) 103
BclI TGATCA 1 cut(s) 529
BfaI CTAG 2 cut(s) 401, 615
BfuAI ACCTGC 1 cut(s) 810
BisI GCNGC 1 cut(s) 27
BlpI GCTNAGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 28
Bme18I GGWCC 1 cut(s) 440
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 1 cut(s) 459
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 374
Bpu1102I GCTNAGC 1 cut(s) 490
BsaJI CCNNGG 2 cut(s) 106, 142
Bse3DI GCAATG 1 cut(s) 638
BseDI CCNNGG 2 cut(s) 106, 142
BseGI GGATG 1 cut(s) 650
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 3 cut(s) 46, 317, 391
BshFI GGCC 1 cut(s) 310
BsiSI CCGG 1 cut(s) 5
BsnI GGCC 1 cut(s) 310
Bsp143I GATC 3 cut(s) 102, 529, 561
Bsp1720I GCTNAGC 1 cut(s) 490
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 2 cut(s) 26, 206
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 1 cut(s) 311
BspLI GGNNCC 1 cut(s) 459
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 97, 569
BsrDI GCAATG 1 cut(s) 638
BssECI CCNNGG 2 cut(s) 106, 142
BssMI GATC 3 cut(s) 102, 529, 561
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 469
BstDEI CTNAG 4 cut(s) 270, 298, 315, 490
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 650
BstKTI GATC 3 cut(s) 105, 532, 564
BstMBI GATC 3 cut(s) 102, 529, 561
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 677
BsuRI GGCC 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 650
BveI ACCTGC 1 cut(s) 810
Cfr13I GGNCC 1 cut(s) 440
Csp6I GTAC 1 cut(s) 580
CviAII CATG 2 cut(s) 107, 674
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 4 cut(s) 270, 298, 315, 490
DpnI GATC 3 cut(s) 104, 531, 563
DpnII GATC 3 cut(s) 102, 529, 561
EaeI YGGCCR 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 110, 677
FaiI YATR 9 cut(s) 108, 233, 249, 276, 464, 512, 540, 675, 788
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 106, 673
FauI CCCGC 1 cut(s) 213
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 637
Fsp4HI GCNGC 1 cut(s) 27
FspBI CTAG 2 cut(s) 401, 615
GluI GCNGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 1 cut(s) 310
HapII CCGG 1 cut(s) 5
Hin1II CATG 2 cut(s) 110, 677
HindIII AAGCTT 3 cut(s) 487, 639, 767
HinfI GANTC 6 cut(s) 75, 111, 200, 296, 421, 591
HpaII CCGG 1 cut(s) 5
Hpy188I TCNGA 4 cut(s) 124, 146, 301, 433
Hpy188III TCNNGA 4 cut(s) 14, 130, 353, 443
Hpy99I CGWCG 2 cut(s) 92, 338
HpyAV CCTTC 1 cut(s) 225
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 7 cut(s) 263, 449, 482, 604, 646, 677, 819
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 4 cut(s) 270, 298, 315, 490
Hsp92II CATG 2 cut(s) 110, 677
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 3 cut(s) 102, 529, 561
LmnI GCTCC 3 cut(s) 7, 355, 457
LweI GCATC 1 cut(s) 436
MaeI CTAG 2 cut(s) 401, 615
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 104, 531, 563
MboI GATC 3 cut(s) 102, 529, 561
MboII GAAGA 2 cut(s) 56, 322
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 7 cut(s) 49, 194, 224, 476, 556, 790, 805
MluNI TGGCCA 1 cut(s) 310
MlyI GAGTC 3 cut(s) 84, 290, 430
MmeI TCCRAC 1 cut(s) 435
Mox20I TGGCCA 1 cut(s) 310
Mph1103I ATGCAT 1 cut(s) 451
MscI TGGCCA 1 cut(s) 310
MseI TTAA 2 cut(s) 69, 759
Msp20I TGGCCA 1 cut(s) 310
MspI CCGG 1 cut(s) 5
MwoI GCNNNNNNNGC 1 cut(s) 35
NcoI CCATGG 1 cut(s) 106
NdeII GATC 3 cut(s) 102, 529, 561
NlaIII CATG 2 cut(s) 110, 677
NlaIV GGNNCC 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 677
PaqCI CACCTGC 1 cut(s) 810
PfeI GAWTC 3 cut(s) 111, 200, 591
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 3 cut(s) 83, 290, 429
PpsI GAGTC 3 cut(s) 83, 290, 429
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 1 cut(s) 440
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SaqAI TTAA 2 cut(s) 69, 759
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 3 cut(s) 102, 529, 561
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 3 cut(s) 84, 290, 430
SfaNI GCATC 1 cut(s) 436
SinI GGWCC 1 cut(s) 440
Sse9I AATT 7 cut(s) 49, 194, 224, 476, 556, 790, 805
SsiI CCGC 2 cut(s) 26, 206
SspMI CTAG 2 cut(s) 401, 615
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 4 cut(s) 21, 192, 336, 394
TasI AATT 7 cut(s) 49, 194, 224, 476, 556, 790, 805
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 111, 200, 591
Tru1I TTAA 2 cut(s) 69, 759
Tru9I TTAA 2 cut(s) 69, 759
TspDTI ATGAA 5 cut(s) 351, 489, 585, 716, 812
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 3 cut(s) 476, 790, 805
XceI RCATGY 1 cut(s) 677
XspI CTAG 2 cut(s) 401, 615
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.