Rroxscaffold_2G00109710

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005648
Physical Location & Seq
Reverse (-)
33891493 .. 33894773
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109710.1

Sequence Viewer

Length: 1281 bp
ATGCCAGAGCTACCGGAGGTGGAGGCGGCCCGGAGGGCCATAGAGGAAAACTGCGTCGGGAAGAAGATCACGAAAGCAATCGTAGCCGACGACTCCAAAGTCATCGACGGAGTCTCGCGCGCGGACTTCGAGGCCTCGCTCTTGGGGAAGACCATCGTCTCCGCGCACCGCAAGGGCAAGAACATGTGGCTCCAACTCGACTCTCCTCCTTTCCCTTCCTTCCAATTCGGGATGGCGGGTGCTGTGTATATTAAAGGGGTTGCGGTCACCAAGTACAAGAGGTCTGCTGTGAAGGACACTGATGAGTGGCCTTCCAAATATTCCAAGGTTTTTGTCCAACTGGATGATGGTCTGGAGTTCTCGTTCACTGACAAGAGGCGATTTGCAAGAGTCCGCCTACTTGAAGATCCAACTTCTGTGCCCCCCATATCTGAGCTCGGGCCCGATGCTCTCTTGGAGCCAATGACAGTGGATGAACTTCTTGCATCATTAAGCAAGAAGAAAATATCAATTAAGGCTCTATTGCTTGATCAGAGCTTTATATCAGGAATTGGCAATTGGGTTGCTGATGAAGTACTATACCAAGCAAGAATTCATCCAGAGCAAAGTTCTGCCACTCTGTCCAAAGAATGTGTTGGAGCTTTACACAAAAGCATCAAAGAGGTTATTGAATATGCAGTCCAAGTTGACGCTGCATCTACTCACTTCCCCTGTGAATGGTTGTTTCATTTTCGTTGGGGCAAAAGGCCTGGAAAAATTAATGTTGAAGTTGGAGCAGATAGTTATCAATATCCTAGTAATTGGATTTTTCATTCCCGTGAAAAGAAACCGGGAACGGTGTTGGTTGATGGGAAGAAGATTAATTTTATCACAGTTGGTGGCAGGACTTCAGCTTTTGTACCAGAGCTTCAGAAGTTAACTGGAAATCAAGCTGCCAAAGCAGGAAGTAAACGAACTAATAAGAGTATTGATGATGTCAATGAAGAAGCAAGTGATGAAGAAGTCAAGGGAACTGCCAAATCTAAGAAAGGTAGGAAGCCTGGAGGTCAGGCCAAGAGGTCTGCCGTGAAAAGAAAATCTAAAGACAGTGATGATGAAGACAATGACAATGATGATTCTGCTGATGATGTCGATCATGAAGACAGTGATGATGATGCTGATGAAGATCGAAAGAACAAAACAAGAAAGGTGACAACTAGTAAGCAATCCAAGGCAGAGACGACATCAAAGAAAAAGGTGCAGACCAGTCAAAATAGTAAACCAAAGAAGAGAGCGAAGTAG

Protein Analysis

426

Amino Acids

47.19

Weight (kDa)

8.93

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 5.6e-29 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 235 2e-28 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 268 - 301 1.9e-19 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccII CGCG 4 cut(s) 118, 120, 122, 164
AciI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 873, 893
AfaI GTAC 3 cut(s) 275, 576, 900
AfiI CCNNNNNNNGG 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 3 cut(s) 404, 671, 767
AhlI ACTAGT 1 cut(s) 1196
AjnI CCWGG 2 cut(s) 748, 1039
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 7 cut(s) 10, 436, 537, 641, 893, 907, 932
AluI AGCT 7 cut(s) 10, 436, 537, 641, 893, 907, 932
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 3 cut(s) 118, 163, 1211
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 7 cut(s) 27, 36, 132, 308, 440, 746, 1050
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 692, 932
ApoI RAATTY 1 cut(s) 591
AseI ATTAAT 2 cut(s) 759, 861
AspLEI GCGC 3 cut(s) 120, 122, 166
AspS9I GGNCC 4 cut(s) 28, 36, 440, 441
AsuC2I CCSGG 2 cut(s) 31, 831
AsuHPI GGTGA 2 cut(s) 259, 1201
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 2 cut(s) 423, 444
BanII GRGCYC 2 cut(s) 438, 444
BarI GAAGNNNNNNTAC 2 cut(s) 891, 923
BbsI GAAGAC 3 cut(s) 155, 1104, 1146
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 679, 919
BccI CCATC 4 cut(s) 161, 226, 341, 842
BceAI ACGGC 1 cut(s) 1049
BciT130I CCWGG 2 cut(s) 750, 1041
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 2 cut(s) 31, 831
BcoDI GTCTC 3 cut(s) 118, 163, 1211
BcuI ACTAGT 1 cut(s) 1196
BfaI CTAG 2 cut(s) 795, 1197
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 3 cut(s) 27, 693, 933
BlsI GCNGC 3 cut(s) 28, 694, 934
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 4 cut(s) 31, 750, 831, 1041
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 4 cut(s) 28, 36, 440, 441
BmiI GGNNCC 3 cut(s) 191, 442, 459
BmrFI CCNGG 4 cut(s) 31, 750, 831, 1041
BmsI GCATC 5 cut(s) 436, 494, 663, 704, 1144
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 3 cut(s) 155, 1104, 1146
BpmI CTGGAG 2 cut(s) 374, 1062
BpuMI CCSGG 2 cut(s) 31, 831
BsaBI GATNNNNATC 3 cut(s) 783, 1131, 1164
BsaJI CCNNGG 2 cut(s) 324, 1209
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 3 cut(s) 345, 925, 1245
Bse8I GATNNNNATC 3 cut(s) 783, 1131, 1164
BseBI CCWGG 2 cut(s) 750, 1041
BseDI CCNNGG 2 cut(s) 324, 1209
BseGI GGATG 4 cut(s) 237, 349, 478, 595
BseJI GATNNNNATC 3 cut(s) 783, 1131, 1164
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 345, 925, 1245
BsePI GCGCGC 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 423, 444
BseXI GCAGC 2 cut(s) 679, 919
BsgI GTGCAG 1 cut(s) 1259
Bsh1236I CGCG 4 cut(s) 118, 120, 122, 164
BshFI GGCC 7 cut(s) 29, 38, 134, 310, 442, 748, 1052
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 3 cut(s) 14, 31, 830
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 3 cut(s) 118, 163, 1211
BsmBI CGTCTC 2 cut(s) 163, 1211
BsnI GGCC 7 cut(s) 29, 38, 134, 310, 442, 748, 1052
BsoBI CYCGRG 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 423, 438, 444
Bsp143I GATC 5 cut(s) 66, 406, 529, 1132, 1165
BspACI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
BspANI GGCC 7 cut(s) 29, 38, 134, 310, 442, 748, 1052
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 4 cut(s) 118, 120, 122, 164
BspHI TCATGA 1 cut(s) 1135
BspLI GGNNCC 3 cut(s) 191, 442, 459
BspPI GGATC 1 cut(s) 401
BsrI ACTGG 3 cut(s) 345, 925, 1245
BssECI CCNNGG 2 cut(s) 324, 1209
BssHII GCGCGC 1 cut(s) 118
BssMI GATC 5 cut(s) 66, 406, 529, 1132, 1165
BssT1I CCWWGG 2 cut(s) 324, 1209
Bst2UI CCWGG 2 cut(s) 750, 1041
Bst4CI ACNGT 5 cut(s) 469, 838, 874, 1088, 1145
Bst6I CTCTTC 1 cut(s) 1262
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 432, 1023
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 4 cut(s) 237, 349, 478, 595
BstFNI CGCG 4 cut(s) 118, 120, 122, 164
BstHHI GCGC 3 cut(s) 120, 122, 166
BstKTI GATC 5 cut(s) 69, 409, 532, 1135, 1168
BstMAI GTCTC 3 cut(s) 118, 163, 1211
BstMBI GATC 5 cut(s) 66, 406, 529, 1132, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 35, 83, 938
BstNI CCWGG 2 cut(s) 750, 1041
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 155
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 29, 748, 829, 1039
BstSLI GKGCMC 2 cut(s) 423, 444
BstUI CGCG 4 cut(s) 118, 120, 122, 164
BstV1I GCAGC 2 cut(s) 679, 919
BstV2I GAAGAC 3 cut(s) 155, 1104, 1146
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 7 cut(s) 29, 38, 134, 310, 442, 748, 1052
BtsCI GGATG 4 cut(s) 237, 349, 478, 595
BtsIMutI CAGTG 5 cut(s) 297, 366, 474, 1093, 1150
Cac8I GCNNGC 1 cut(s) 120
CciI TCATGA 1 cut(s) 1135
CfoI GCGC 3 cut(s) 120, 122, 166
Cfr13I GGNCC 4 cut(s) 28, 36, 440, 441
CseI GACGC 2 cut(s) 43, 698
Csp6I GTAC 3 cut(s) 274, 575, 899
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviAII CATG 2 cut(s) 184, 1136
CviQI GTAC 3 cut(s) 274, 575, 899
DdeI CTNAG 2 cut(s) 432, 1023
DpnI GATC 5 cut(s) 68, 408, 531, 1134, 1167
DpnII GATC 5 cut(s) 66, 406, 529, 1132, 1165
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 1262
EarI CTCTTC 1 cut(s) 1262
EciI GGCGGA 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 324, 1209
Eco147I AGGCCT 2 cut(s) 134, 748
Eco24I GRGCYC 2 cut(s) 438, 444
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 873, 893
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 748, 1039
EcoT14I CCWWGG 2 cut(s) 324, 1209
EcoT38I GRGCYC 2 cut(s) 438, 444
ErhI CCWWGG 2 cut(s) 324, 1209
Esp3I CGTCTC 2 cut(s) 163, 1211
FaeI CATG 2 cut(s) 187, 1139
FaiI YATR 8 cut(s) 41, 185, 249, 428, 542, 580, 675, 1137
FatI CATG 2 cut(s) 183, 1135
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 3 cut(s) 27, 693, 933
FokI GGATG 4 cut(s) 244, 356, 485, 582
FriOI GRGCYC 2 cut(s) 438, 444
Fsp4HI GCNGC 3 cut(s) 27, 693, 933
FspBI CTAG 2 cut(s) 795, 1197
GlaI GCGC 3 cut(s) 119, 121, 165
GluI GCNGC 3 cut(s) 27, 693, 933
GsuI CTGGAG 2 cut(s) 374, 1062
HaeIII GGCC 7 cut(s) 29, 38, 134, 310, 442, 748, 1052
HapII CCGG 3 cut(s) 14, 31, 830
HgaI GACGC 2 cut(s) 43, 698
HhaI GCGC 3 cut(s) 120, 122, 166
Hin1II CATG 2 cut(s) 187, 1139
Hin6I GCGC 3 cut(s) 118, 120, 164
HinP1I GCGC 3 cut(s) 118, 120, 164
HincII GTYRAC 2 cut(s) 688, 918
HindII GTYRAC 2 cut(s) 688, 918
HinfI GANTC 5 cut(s) 92, 111, 200, 390, 1115
HpaI GTTAAC 1 cut(s) 918
HpaII CCGG 3 cut(s) 14, 31, 830
HphI GGTGA 2 cut(s) 259, 1201
Hpy166II GTNNAC 5 cut(s) 366, 688, 918, 950, 1259
Hpy188I TCNGA 3 cut(s) 433, 534, 912
Hpy188III TCNNGA 7 cut(s) 58, 70, 229, 353, 546, 599, 1136
Hpy8I GTNNAC 5 cut(s) 366, 688, 918, 950, 1259
Hpy99I CGWCG 3 cut(s) 59, 92, 110
HpyAV CCTTC 4 cut(s) 225, 229, 286, 321
HpyCH4III ACNGT 5 cut(s) 469, 838, 874, 1088, 1145
HpyCH4V TGCA 5 cut(s) 386, 485, 677, 695, 1240
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 83, 938
HpyF3I CTNAG 2 cut(s) 432, 1023
Hsp92II CATG 2 cut(s) 187, 1139
HspAI GCGC 3 cut(s) 118, 120, 164
Ksp22I TGATCA 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 918
Kzo9I GATC 5 cut(s) 66, 406, 529, 1132, 1165
LmnI GCTCC 4 cut(s) 195, 457, 638, 773
Lsp1109I GCAGC 2 cut(s) 679, 919
LweI GCATC 5 cut(s) 436, 494, 663, 704, 1144
MaeI CTAG 2 cut(s) 795, 1197
MaeIII GTNAC 2 cut(s) 265, 1189
MalI GATC 5 cut(s) 68, 408, 531, 1134, 1167
MauBI CGCGCGCG 1 cut(s) 118
MboI GATC 5 cut(s) 66, 406, 529, 1132, 1165
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 3 cut(s) 423, 438, 444
MluCI AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 799, 862
MlyI GAGTC 4 cut(s) 86, 120, 194, 399
MmeI TCCRAC 5 cut(s) 217, 361, 434, 616, 751
MseI TTAA 6 cut(s) 252, 491, 513, 759, 861, 917
MslI CAYNNNNRTG 1 cut(s) 816
MspI CCGG 3 cut(s) 14, 31, 830
MspR9I CCNGG 4 cut(s) 31, 750, 831, 1041
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 2 cut(s) 750, 1041
MvnI CGCG 4 cut(s) 118, 120, 122, 164
MwoI GCNNNNNNNGC 3 cut(s) 35, 83, 938
NciI CCSGG 2 cut(s) 31, 831
NdeII GATC 5 cut(s) 66, 406, 529, 1132, 1165
NlaIII CATG 2 cut(s) 187, 1139
NlaIV GGNNCC 3 cut(s) 191, 442, 459
NmuCI GTSAC 2 cut(s) 265, 1189
NspI RCATGY 1 cut(s) 187
PagI TCATGA 1 cut(s) 1135
PauI GCGCGC 1 cut(s) 118
PceI AGGCCT 2 cut(s) 134, 748
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 1 cut(s) 1115
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 28, 694, 934
PleI GAGTC 4 cut(s) 86, 119, 194, 398
PpsI GAGTC 4 cut(s) 86, 119, 194, 398
PscI ACATGT 1 cut(s) 183
PshAI GACNNNNGTC 1 cut(s) 155
PshBI ATTAAT 2 cut(s) 759, 861
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 2 cut(s) 748, 1039
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 748, 1039
PspN4I GGNNCC 3 cut(s) 191, 442, 459
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 4 cut(s) 28, 36, 440, 441
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 1 cut(s) 110
PteI GCGCGC 1 cut(s) 118
RsaI GTAC 3 cut(s) 275, 576, 900
RsaNI GTAC 3 cut(s) 274, 575, 899
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 6 cut(s) 252, 491, 513, 759, 861, 917
SatI GCNGC 3 cut(s) 27, 693, 933
Sau3AI GATC 5 cut(s) 66, 406, 529, 1132, 1165
Sau96I GGNCC 4 cut(s) 28, 36, 440, 441
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 4 cut(s) 86, 120, 194, 399
ScrFI CCNGG 4 cut(s) 31, 750, 831, 1041
SduI GDGCHC 3 cut(s) 423, 438, 444
SfaNI GCATC 5 cut(s) 436, 494, 663, 704, 1144
SfiI GGCCNNNNNGGCC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 816
SpeI ACTAGT 1 cut(s) 1196
Sse9I AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 799, 862
SseBI AGGCCT 2 cut(s) 134, 748
SsiI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
SspI AATATT 1 cut(s) 320
SspMI CTAG 2 cut(s) 795, 1197
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 2 cut(s) 134, 748
StyD4I CCNGG 4 cut(s) 29, 748, 829, 1039
StyI CCWWGG 2 cut(s) 324, 1209
TaaI ACNGT 5 cut(s) 469, 838, 874, 1088, 1145
TaqI TCGA 5 cut(s) 105, 129, 198, 1131, 1168
TasI AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 799, 862
TatI WGTACW 2 cut(s) 273, 574
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 1115
Tru1I TTAA 6 cut(s) 252, 491, 513, 759, 861, 917
Tru9I TTAA 6 cut(s) 252, 491, 513, 759, 861, 917
TscAI CASTG 5 cut(s) 304, 373, 474, 1093, 1150
TseFI GTSAC 2 cut(s) 265, 1189
TseI GCWGC 2 cut(s) 692, 932
Tsp45I GTSAC 2 cut(s) 265, 1189
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 5 cut(s) 304, 373, 474, 1093, 1150
Tth111I GACNNNGTC 1 cut(s) 110
VspI ATTAAT 2 cut(s) 759, 861
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XspI CTAG 2 cut(s) 795, 1197
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.