Rh2CG356600

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005648
Physical Location & Seq
Forward (+)
48314483 .. 48320480
5998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG356600.1

Sequence Viewer

Length: 945 bp
ATGCCAGAGCTACCGGAGGTGGAGGCGGCCCGGAGGGCCATAGAGGAAAACTGCGTCGGGAAGAAGATCACGAAAGCAATCGTCGCCGACGACTCCAAAGTCATCGACGGAGTTTCGCGCGCGGACTTCGAGGCCTCGCTCTTGGGGAAGACCATCGTCTCCGCGCACCGCAAGGGCAAGAACATGTGGCTCCAACTCGACTCTCCTCCTTTCCCTTCCTTCCAATTCGGGATGGCGGGTGCTGTGTATATTAAAGGGGTTGCGGTCACCAAGTACAAGAGGTCTGCTGTGAAGGACACTGATGAGTGGCCTTCCAAATATTCCAAGGTTTTTGTCCAACTGGATGATGGTCTGGAGTTCTCGTTCACTGACAAGAGGCGATTTGCAAGAGTCCGCCTACTTGAAGATCCAACTTCTGTGCCCCCCATATCTGAGCTCGGGCCCGATGCTCTCTTGGAGCCAATGACAGTAGATGAACTTCTTGCATCATTAAGCAAGAAGAAAATATCAATTAAGGCTCTATTGCTTGATCAGAGCTTTATATCAGGAATTGGCAATTGGGTTGCTGATGAAGTACTATACCAAGCAAGAATTCATCCGGAGCAAAGTTCTGCCACCCTGTCCAAAGAATGTGTTGGAGCCTTACACAAAAGCATCAAAGAGGTTATTGAATATGCAGTCCAAGTTGACGCTGCATCTACTCACTTCCCCTGTGAATGGTTGTTTCATTTTCGTTGGGGCAAAAGGCCTGGAAAAATTAATGGTAAGAAATTGGTGATATATGTGGCTTTTCTTTTACAGGTCATTGAAAAATCAGTTGAAGTTGGAGCAGATAGTGATCAATATCCTAGTAATTGGATTTTTCATTCCCGTGAAAAGAAACCGGGAAAGGTGTTGGTTGATGGTTTGGTTCCTGACCCCTTATCCATGCAACCTTTTTTTTAA

Protein Analysis

314

Amino Acids

35.02

Weight (kDa)

7.68

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 2.9e-29 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 235 1.1e-28 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 286 - 302 1.3e-07 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccII CGCG 4 cut(s) 118, 120, 122, 164
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 591
AfaI GTAC 2 cut(s) 275, 576
AfiI CCNNNNNNNGG 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 4 cut(s) 404, 671, 809, 821
AjnI CCWGG 1 cut(s) 748
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 3 cut(s) 10, 436, 537
AluI AGCT 3 cut(s) 10, 436, 537
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 6 cut(s) 27, 36, 132, 308, 440, 746
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 1 cut(s) 591
AseI ATTAAT 1 cut(s) 759
AspLEI GCGC 3 cut(s) 120, 122, 166
AspS9I GGNCC 4 cut(s) 28, 36, 440, 441
AsuC2I CCSGG 2 cut(s) 31, 885
AsuHPI GGTGA 2 cut(s) 259, 787
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 2 cut(s) 423, 444
BanII GRGCYC 2 cut(s) 438, 444
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 679
BccI CCATC 4 cut(s) 161, 226, 341, 896
BciT130I CCWGG 1 cut(s) 750
BclI TGATCA 2 cut(s) 529, 838
BcnI CCSGG 2 cut(s) 31, 885
BcoDI GTCTC 1 cut(s) 163
BfaI CTAG 1 cut(s) 849
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 2 cut(s) 27, 693
BlsI GCNGC 2 cut(s) 28, 694
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 3 cut(s) 31, 750, 885
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 4 cut(s) 28, 36, 440, 441
BmiI GGNNCC 5 cut(s) 191, 442, 459, 640, 912
BmrFI CCNGG 3 cut(s) 31, 750, 885
BmsI GCATC 4 cut(s) 436, 494, 663, 704
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 1 cut(s) 374
BpuMI CCSGG 2 cut(s) 31, 885
BsaBI GATNNNNATC 2 cut(s) 837, 843
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 2 cut(s) 13, 598
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 1 cut(s) 345
Bse8I GATNNNNATC 2 cut(s) 837, 843
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 1 cut(s) 750
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 237, 349, 595
BseJI GATNNNNATC 2 cut(s) 837, 843
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 345
BsePI GCGCGC 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 423, 444
BseXI GCAGC 1 cut(s) 679
Bsh1236I CGCG 4 cut(s) 118, 120, 122, 164
BshFI GGCC 6 cut(s) 29, 38, 134, 310, 442, 748
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 4 cut(s) 14, 31, 599, 884
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 1 cut(s) 163
BsmBI CGTCTC 1 cut(s) 163
BsnI GGCC 6 cut(s) 29, 38, 134, 310, 442, 748
BsoBI CYCGRG 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 423, 438, 444
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 4 cut(s) 66, 406, 529, 838
BspACI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
BspANI GGCC 6 cut(s) 29, 38, 134, 310, 442, 748
BspCNI CTCAG 1 cut(s) 424
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 4 cut(s) 118, 120, 122, 164
BspLI GGNNCC 5 cut(s) 191, 442, 459, 640, 912
BspPI GGATC 1 cut(s) 401
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 324
BssHII GCGCGC 1 cut(s) 118
BssMI GATC 4 cut(s) 66, 406, 529, 838
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 750
Bst4CI ACNGT 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 432
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 3 cut(s) 237, 349, 595
BstFNI CGCG 4 cut(s) 118, 120, 122, 164
BstHHI GCGC 3 cut(s) 120, 122, 166
BstKTI GATC 4 cut(s) 69, 409, 532, 841
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 4 cut(s) 66, 406, 529, 838
BstMWI GCNNNNNNNGC 2 cut(s) 35, 83
BstNI CCWGG 1 cut(s) 750
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 155
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 29, 748, 883
BstSLI GKGCMC 2 cut(s) 423, 444
BstUI CGCG 4 cut(s) 118, 120, 122, 164
BstV1I GCAGC 1 cut(s) 679
BstV2I GAAGAC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 6 cut(s) 29, 38, 134, 310, 442, 748
BtsCI GGATG 3 cut(s) 237, 349, 595
BtsIMutI CAGTG 2 cut(s) 297, 366
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 3 cut(s) 120, 122, 166
Cfr13I GGNCC 4 cut(s) 28, 36, 440, 441
CseI GACGC 2 cut(s) 43, 698
Csp6I GTAC 2 cut(s) 274, 575
CviAII CATG 2 cut(s) 184, 928
CviQI GTAC 2 cut(s) 274, 575
DdeI CTNAG 1 cut(s) 432
DpnI GATC 4 cut(s) 68, 408, 531, 840
DpnII GATC 4 cut(s) 66, 406, 529, 838
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 324
Eco147I AGGCCT 2 cut(s) 134, 748
Eco24I GRGCYC 2 cut(s) 438, 444
Eco53kI GAGCTC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 438, 444
ErhI CCWWGG 1 cut(s) 324
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 2 cut(s) 187, 931
FatI CATG 2 cut(s) 183, 927
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 2 cut(s) 529, 838
Fnu4HI GCNGC 2 cut(s) 27, 693
FokI GGATG 3 cut(s) 244, 356, 582
FriOI GRGCYC 2 cut(s) 438, 444
Fsp4HI GCNGC 2 cut(s) 27, 693
FspBI CTAG 1 cut(s) 849
GlaI GCGC 3 cut(s) 119, 121, 165
GluI GCNGC 2 cut(s) 27, 693
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 6 cut(s) 29, 38, 134, 310, 442, 748
HapII CCGG 4 cut(s) 14, 31, 599, 884
HgaI GACGC 2 cut(s) 43, 698
HhaI GCGC 3 cut(s) 120, 122, 166
Hin1II CATG 2 cut(s) 187, 931
Hin6I GCGC 3 cut(s) 118, 120, 164
HinP1I GCGC 3 cut(s) 118, 120, 164
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HinfI GANTC 3 cut(s) 92, 200, 390
HpaII CCGG 4 cut(s) 14, 31, 599, 884
HphI GGTGA 2 cut(s) 259, 787
Hpy166II GTNNAC 2 cut(s) 366, 688
Hpy188I TCNGA 2 cut(s) 433, 534
Hpy188III TCNNGA 7 cut(s) 58, 70, 229, 353, 546, 599, 914
Hpy8I GTNNAC 2 cut(s) 366, 688
Hpy99I CGWCG 4 cut(s) 59, 86, 92, 110
HpyAV CCTTC 4 cut(s) 225, 229, 286, 321
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 5 cut(s) 386, 485, 677, 695, 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 83
HpyF3I CTNAG 1 cut(s) 432
Hsp92II CATG 2 cut(s) 187, 931
HspAI GCGC 3 cut(s) 118, 120, 164
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 2 cut(s) 529, 838
Kzo9I GATC 4 cut(s) 66, 406, 529, 838
LmnI GCTCC 5 cut(s) 195, 457, 601, 638, 827
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 4 cut(s) 436, 494, 663, 704
MaeI CTAG 1 cut(s) 849
MaeIII GTNAC 1 cut(s) 265
MalI GATC 4 cut(s) 68, 408, 531, 840
MauBI CGCGCGCG 1 cut(s) 118
MboI GATC 4 cut(s) 66, 406, 529, 838
MboII GAAGA 5 cut(s) 73, 76, 160, 416, 511
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 3 cut(s) 423, 438, 444
MluCI AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 770, 853
MlyI GAGTC 3 cut(s) 86, 194, 399
MmeI TCCRAC 5 cut(s) 217, 361, 434, 616, 805
MroI TCCGGA 1 cut(s) 598
MseI TTAA 5 cut(s) 252, 491, 513, 759, 943
MslI CAYNNNNRTG 1 cut(s) 870
MspI CCGG 4 cut(s) 14, 31, 599, 884
MspR9I CCNGG 3 cut(s) 31, 750, 885
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 1 cut(s) 750
MvnI CGCG 4 cut(s) 118, 120, 122, 164
MwoI GCNNNNNNNGC 2 cut(s) 35, 83
NciI CCSGG 2 cut(s) 31, 885
NdeII GATC 4 cut(s) 66, 406, 529, 838
NlaIII CATG 2 cut(s) 187, 931
NlaIV GGNNCC 5 cut(s) 191, 442, 459, 640, 912
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 187
PauI GCGCGC 1 cut(s) 118
PceI AGGCCT 2 cut(s) 134, 748
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PkrI GCNGC 2 cut(s) 28, 694
PleI GAGTC 3 cut(s) 86, 194, 398
PpsI GAGTC 3 cut(s) 86, 194, 398
PscI ACATGT 1 cut(s) 183
PshAI GACNNNNGTC 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 748
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 748
PspN4I GGNNCC 5 cut(s) 191, 442, 459, 640, 912
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 4 cut(s) 28, 36, 440, 441
PsuI RGATCY 1 cut(s) 406
PteI GCGCGC 1 cut(s) 118
RsaI GTAC 2 cut(s) 275, 576
RsaNI GTAC 2 cut(s) 274, 575
RseI CAYNNNNRTG 1 cut(s) 870
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 5 cut(s) 252, 491, 513, 759, 943
SatI GCNGC 2 cut(s) 27, 693
Sau3AI GATC 4 cut(s) 66, 406, 529, 838
Sau96I GGNCC 4 cut(s) 28, 36, 440, 441
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 3 cut(s) 86, 194, 399
ScrFI CCNGG 3 cut(s) 31, 750, 885
SduI GDGCHC 3 cut(s) 423, 438, 444
SfaNI GCATC 4 cut(s) 436, 494, 663, 704
SfiI GGCCNNNNNGGCC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 870
Sse9I AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 770, 853
SseBI AGGCCT 2 cut(s) 134, 748
SsiI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
SspI AATATT 1 cut(s) 320
SspMI CTAG 1 cut(s) 849
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 2 cut(s) 134, 748
StyD4I CCNGG 3 cut(s) 29, 748, 883
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 3 cut(s) 105, 129, 198
TasI AATT 8 cut(s) 224, 510, 549, 556, 591, 756, 770, 853
TatI WGTACW 2 cut(s) 273, 574
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 5 cut(s) 252, 491, 513, 759, 943
Tru9I TTAA 5 cut(s) 252, 491, 513, 759, 943
TscAI CASTG 2 cut(s) 304, 373
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 5 cut(s) 489, 584, 585, 716, 854
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 2 cut(s) 304, 373
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XspI CTAG 1 cut(s) 849
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.