FvH4_6g29401

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005648
Physical Location & Seq
Forward (+)
22685775 .. 22690779
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29401.t5

Sequence Viewer

Length: 1191 bp
ATGCCAGAGCTACCGGAGGTGGAGGCGGCCCGGAGGGCCATACAGGACAACTGCGTCGGGAAGAAGATCACGAAAGCGATCGTCGCCGACGACTCCAAAGTCATCGACGGAGTCTCGCGCGCGGACTTCGAGGCATCGCTATTGGGGAAGACCATCATCTCCGCCCACCGTAAGGGCAAGAACATGTGGCTCCAACTCGACTCCCCTCCTTTCCCTTCCTTCCAATTCGGGATGGCGGGTGCGGTGTATATTAAAGGGGTTGCGGTCACCAAATACAAGAGATCTGCTGTTAAGGACACTGATGAGTGGCCGTCCAAATACTCCAAGGTTTTTGTCCAACTGGATGACGGTCTGGAGTTCTCATTCACTGACAAAAGGCGATTTGCAAAAGTGCGCCTGCTCAAAGATCCAACTTCTGTGCCCCCCATATCTGAGCTTGGGCCTGATGCTCTCTTGGAGCCAATGACTGTCGATGTACTATTTGCATCATTAAGCAAGAAGAAAATATCAATTAAGGCTCTATTGCTTGATCAGAGCTTTATATCAGGAATTGGCAATTGGGTTGCTGATGAAGTACTATACCAAGCAAGAATTCATCCGGAGCAAAGTTCTGCCACCCTGTCCAAAGAATGTGTCGAAGCTTTACACAAAAGCATCAAAGAGGTTATTGAATATGCGGTTCAAGTTGACGCTGAATCTATCCACTTCCCCTGTGAATGGTTGTTTCATTTTCGTTGGGGCAAAAGGGCTGGAAAAATTAATGGGAAGAAAATTGATTTTATCACAGTTGGTGGCAGGACTTCAGCGTTTGTACCAGAGCTTCAGAAGTTAACTGGAAATCAAGCTGCCAATGCAGAAAGTAAAAGAACTAGTAAGCGTGTAGATGATGTTGATGAAGAAGCAAGTGATAAAGAAGTCAAGGGAACTGCCAAATCTAGGAAAGGGAGGAAGCCTGGAGGTCAGGCCAAGAGGTCTACAGTGAAAAGAAAATCAAAAGGCAGTGATGAAGACAATGACAATGATGATTCCACTGATGATGTCGATCATGATGACAATGATGATTCTGCTGATGAAGATATGAAGAACAGAACAAGAAAGGTGACAACTAATAAGCAATCCAAAGCAGAGATGACATCTAAGAAAAAGAAGCAGACCAGTCAAAATGCCAAACCTAAGAAGAGAGCGAAGTAG

Protein Analysis

397

Amino Acids

44.04

Weight (kDa)

9.28

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 9.4e-29 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 235 8.6e-30 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 240 - 256 1e-06 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 53, 98
AccI GTMKAC 1 cut(s) 974
AccII CGCG 3 cut(s) 118, 120, 122
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 7 cut(s) 26, 122, 162, 236, 242, 263, 677
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 786, 806
AfaI GTAC 3 cut(s) 477, 576, 813
AfiI CCNNNNNNNGG 3 cut(s) 172, 717, 936
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 671, 683
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 1 cut(s) 952
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AluBI AGCT 6 cut(s) 10, 436, 537, 641, 820, 845
AluI AGCT 6 cut(s) 10, 436, 537, 641, 820, 845
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 5 cut(s) 27, 36, 308, 440, 963
ApeKI GCWGC 1 cut(s) 845
ApoI RAATTY 1 cut(s) 591
ArsI GACNNNNNNTTYG 2 cut(s) 618, 650
AseI ATTAAT 1 cut(s) 759
AspLEI GCGC 3 cut(s) 120, 122, 396
AspS9I GGNCC 3 cut(s) 28, 36, 440
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 2 cut(s) 259, 1111
BaeGI GKGCMC 1 cut(s) 423
BarI GAAGNNNNNNTAC 2 cut(s) 804, 836
BbsI GAAGAC 2 cut(s) 155, 1014
BbvI GCAGC 1 cut(s) 832
BccI CCATC 2 cut(s) 161, 226
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 1 cut(s) 954
BclI TGATCA 1 cut(s) 529
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 118
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 2 cut(s) 870, 936
BfmI CTRYAG 1 cut(s) 975
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 281
BisI GCNGC 2 cut(s) 27, 846
BlsI GCNGC 2 cut(s) 28, 847
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 2 cut(s) 31, 954
BmgT120I GGNCC 3 cut(s) 28, 36, 440
BmiI GGNNCC 2 cut(s) 191, 459
BmrFI CCNGG 2 cut(s) 31, 954
BmsI GCATC 4 cut(s) 143, 436, 494, 663
BpiI GAAGAC 2 cut(s) 155, 1014
BpmI CTGGAG 2 cut(s) 374, 975
BpuMI CCSGG 1 cut(s) 31
BsaBI GATNNNNATC 1 cut(s) 1041
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 2 cut(s) 13, 598
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 717, 936
Bse1I ACTGG 3 cut(s) 345, 838, 1155
Bse8I GATNNNNATC 1 cut(s) 1041
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 1 cut(s) 954
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 237, 349, 595
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 3 cut(s) 172, 717, 936
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 345, 838, 1155
BsePI GCGCGC 1 cut(s) 118
BseSI GKGCMC 1 cut(s) 423
BseXI GCAGC 1 cut(s) 832
Bsh1236I CGCG 3 cut(s) 118, 120, 122
Bsh1285I CGRYCG 1 cut(s) 81
BshFI GGCC 5 cut(s) 29, 38, 310, 442, 965
BsiEI CGRYCG 1 cut(s) 81
BsiSI CCGG 3 cut(s) 14, 31, 599
BslI CCNNNNNNNGG 3 cut(s) 172, 717, 936
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 5 cut(s) 29, 38, 310, 442, 965
Bsp1286I GDGCHC 1 cut(s) 423
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
BspACI CCGC 7 cut(s) 26, 122, 162, 236, 242, 263, 677
BspANI GGCC 5 cut(s) 29, 38, 310, 442, 965
BspCNI CTCAG 1 cut(s) 424
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 3 cut(s) 118, 120, 122
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 2 cut(s) 191, 459
BspPI GGATC 1 cut(s) 401
BsrI ACTGG 3 cut(s) 345, 838, 1155
BssECI CCNNGG 1 cut(s) 324
BssHII GCGCGC 1 cut(s) 118
BssMI GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 954
Bst4CI ACNGT 5 cut(s) 170, 350, 469, 787, 979
Bst6I CTCTTC 1 cut(s) 1172
BstC8I GCNNGC 2 cut(s) 120, 398
BstDEI CTNAG 3 cut(s) 432, 1137, 1173
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 3 cut(s) 237, 349, 595
BstFNI CGCG 3 cut(s) 118, 120, 122
BstHHI GCGC 3 cut(s) 120, 122, 396
BstKTI GATC 6 cut(s) 69, 81, 284, 409, 532, 1045
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
BstMCI CGRYCG 1 cut(s) 81
BstMWI GCNNNNNNNGC 3 cut(s) 35, 83, 851
BstNI CCWGG 1 cut(s) 954
BstNSI RCATGY 1 cut(s) 187
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 29, 952
BstSFI CTRYAG 1 cut(s) 975
BstSLI GKGCMC 1 cut(s) 423
BstUI CGCG 3 cut(s) 118, 120, 122
BstV1I GCAGC 1 cut(s) 832
BstV2I GAAGAC 2 cut(s) 155, 1014
BstX2I RGATCY 2 cut(s) 281, 406
BstYI RGATCY 2 cut(s) 281, 406
BsuRI GGCC 5 cut(s) 29, 38, 310, 442, 965
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 3 cut(s) 237, 349, 595
BtsI GCAGTG 1 cut(s) 1006
BtsIMutI CAGTG 5 cut(s) 297, 366, 984, 1006, 1029
Cac8I GCNNGC 2 cut(s) 120, 398
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 3 cut(s) 120, 122, 396
Cfr13I GGNCC 3 cut(s) 28, 36, 440
CseI GACGC 2 cut(s) 43, 698
Csp6I GTAC 3 cut(s) 476, 575, 812
CviAII CATG 2 cut(s) 184, 1046
CviQI GTAC 3 cut(s) 476, 575, 812
DdeI CTNAG 3 cut(s) 432, 1137, 1173
DpnI GATC 6 cut(s) 68, 80, 283, 408, 531, 1044
DpnII GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
DrdI GACNNNNNNGTC 2 cut(s) 53, 98
DseDI GACNNNNNNGTC 2 cut(s) 53, 98
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
EciI GGCGGA 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 324
Eco57I CTGAAG 2 cut(s) 786, 806
Eco91I GGTNACC 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 952
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 2 cut(s) 187, 1049
FaiI YATR 9 cut(s) 41, 185, 249, 428, 542, 580, 675, 1047, 1079
FatI CATG 2 cut(s) 183, 1045
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 529
FblI GTMKAC 1 cut(s) 974
Fnu4HI GCNGC 2 cut(s) 27, 846
FokI GGATG 3 cut(s) 244, 356, 582
Fsp4HI GCNGC 2 cut(s) 27, 846
FspBI CTAG 2 cut(s) 870, 936
GlaI GCGC 3 cut(s) 119, 121, 395
GluI GCNGC 2 cut(s) 27, 846
GsuI CTGGAG 2 cut(s) 374, 975
HaeIII GGCC 5 cut(s) 29, 38, 310, 442, 965
HapII CCGG 3 cut(s) 14, 31, 599
HgaI GACGC 2 cut(s) 43, 698
HhaI GCGC 3 cut(s) 120, 122, 396
Hin1II CATG 2 cut(s) 187, 1049
Hin6I GCGC 3 cut(s) 118, 120, 394
HinP1I GCGC 3 cut(s) 118, 120, 394
HincII GTYRAC 2 cut(s) 688, 831
HindII GTYRAC 2 cut(s) 688, 831
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 6 cut(s) 92, 111, 200, 695, 1025, 1061
HpaI GTTAAC 1 cut(s) 831
HpaII CCGG 3 cut(s) 14, 31, 599
HphI GGTGA 2 cut(s) 259, 1111
Hpy166II GTNNAC 3 cut(s) 688, 831, 975
Hpy188I TCNGA 3 cut(s) 433, 534, 825
Hpy188III TCNNGA 7 cut(s) 58, 70, 229, 353, 546, 599, 1046
Hpy8I GTNNAC 3 cut(s) 688, 831, 975
Hpy99I CGWCG 4 cut(s) 59, 86, 92, 110
HpyAV CCTTC 2 cut(s) 225, 229
HpyCH4III ACNGT 5 cut(s) 170, 350, 469, 787, 979
HpyCH4V TGCA 3 cut(s) 386, 485, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 83, 851
HpyF3I CTNAG 3 cut(s) 432, 1137, 1173
Hsp92II CATG 2 cut(s) 187, 1049
HspAI GCGC 3 cut(s) 118, 120, 394
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 831
Kzo9I GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
LmnI GCTCC 3 cut(s) 195, 457, 601
Lsp1109I GCAGC 1 cut(s) 832
LweI GCATC 4 cut(s) 143, 436, 494, 663
MaeI CTAG 2 cut(s) 870, 936
MaeIII GTNAC 2 cut(s) 265, 1099
MalI GATC 6 cut(s) 68, 80, 283, 408, 531, 1044
MauBI CGCGCGCG 1 cut(s) 118
MboI GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 2 cut(s) 281, 406
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 7 cut(s) 224, 510, 549, 556, 591, 756, 771
MlyI GAGTC 3 cut(s) 86, 120, 194
MmeI TCCRAC 3 cut(s) 217, 361, 434
MnlI CCTC 9 cut(s) 10, 16, 27, 124, 216, 655, 939, 950, 963
MroI TCCGGA 1 cut(s) 598
MseI TTAA 6 cut(s) 252, 291, 491, 513, 759, 830
MspI CCGG 3 cut(s) 14, 31, 599
MspR9I CCNGG 2 cut(s) 31, 954
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 1 cut(s) 954
MvnI CGCG 3 cut(s) 118, 120, 122
MwoI GCNNNNNNNGC 3 cut(s) 35, 83, 851
NciI CCSGG 1 cut(s) 31
NdeII GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
NlaIII CATG 2 cut(s) 187, 1049
NlaIV GGNNCC 2 cut(s) 191, 459
NmuCI GTSAC 2 cut(s) 265, 1099
NspI RCATGY 1 cut(s) 187
PagI TCATGA 1 cut(s) 1045
PauI GCGCGC 1 cut(s) 118
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 3 cut(s) 695, 1025, 1061
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 2 cut(s) 28, 847
Ple19I CGATCG 1 cut(s) 81
PleI GAGTC 3 cut(s) 86, 119, 194
PpsI GAGTC 3 cut(s) 86, 119, 194
PscI ACATGT 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 952
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 952
PspN4I GGNNCC 2 cut(s) 191, 459
PspPI GGNCC 3 cut(s) 28, 36, 440
PsuI RGATCY 2 cut(s) 281, 406
PsyI GACNNNGTC 1 cut(s) 110
PteI GCGCGC 1 cut(s) 118
PvuI CGATCG 1 cut(s) 81
RsaI GTAC 3 cut(s) 477, 576, 813
RsaNI GTAC 3 cut(s) 476, 575, 812
SaqAI TTAA 6 cut(s) 252, 291, 491, 513, 759, 830
SatI GCNGC 2 cut(s) 27, 846
Sau3AI GATC 6 cut(s) 66, 78, 281, 406, 529, 1042
Sau96I GGNCC 3 cut(s) 28, 36, 440
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 3 cut(s) 86, 120, 194
ScrFI CCNGG 2 cut(s) 31, 954
SduI GDGCHC 1 cut(s) 423
SfaNI GCATC 4 cut(s) 143, 436, 494, 663
SfcI CTRYAG 1 cut(s) 975
SfiI GGCCNNNNNGGCC 1 cut(s) 35
SpeI ACTAGT 1 cut(s) 869
Sse9I AATT 7 cut(s) 224, 510, 549, 556, 591, 756, 771
SsiI CCGC 7 cut(s) 26, 122, 162, 236, 242, 263, 677
SspMI CTAG 2 cut(s) 870, 936
StyD4I CCNGG 2 cut(s) 29, 952
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 5 cut(s) 170, 350, 469, 787, 979
TaqI TCGA 6 cut(s) 105, 129, 198, 471, 636, 1041
TasI AATT 7 cut(s) 224, 510, 549, 556, 591, 756, 771
TatI WGTACW 2 cut(s) 475, 574
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 695, 1025, 1061
Tru1I TTAA 6 cut(s) 252, 291, 491, 513, 759, 830
Tru9I TTAA 6 cut(s) 252, 291, 491, 513, 759, 830
TscAI CASTG 5 cut(s) 304, 373, 984, 1006, 1036
TseFI GTSAC 2 cut(s) 265, 1099
TseI GCWGC 1 cut(s) 845
Tsp45I GTSAC 2 cut(s) 265, 1099
TspDTI ATGAA 7 cut(s) 584, 585, 716, 909, 1020, 1086, 1094
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 5 cut(s) 304, 373, 984, 1006, 1036
Tth111I GACNNNGTC 1 cut(s) 110
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 974
XspI CTAG 2 cut(s) 870, 936
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.