MD17G1215000.v1.1

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005648
Physical Location & Seq
Reverse (-)
26188458 .. 26192507
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1215000.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGCCGGAGCTACCGGAGGTGGAGGCGGCTCGGAGGGCCATAGAGGAACACTGCGTAGGGAAGAAGATAACGAAAGCACTCATCGCCGACGACTCCAAAGTCATCGATGGAGTCTCACGCGCCGACTTCGAGGCCTCTCTCTTGGGAAAGACCATCGTCTCCGCCCACCGCAAGGGCAAGAACATGTGGCTCTGCCTCGACTCTCCTCCCTTTCCTTCCTTCCAATTCGGAATGGCGGGTGCTGTATATATTAAAGGGGTCGCCGTCACGAAATATAAGAGGTCTGCTGTAAAGGACACCGATGAGTGGCCTTCCAAGTATTCCAAGTTCTTTGTTGAATTGGATGATGGTCTGGAGTTCTCTTTCACTGACAAGAGGCGATTTGCTAGAGTCCGCTTGCTGAAAGATCCAACTTCTGTGCCCCCCATATCTGAACTCGGGCCCGATGCTCTCTTGGAGCCTATGACAGTGAATGAACTTTTTGAATGCTTCAGCAAGAAGAAAATTGCGATTAAGACCCTGTTGCTTGATCAGAGTGTTATTTCAGGAATTGGCAATTGGGTTGCTGATGAAGTACTCTACCAAGCAAGAATTCATCCGGAGCAAAGTGCTGCCAGCCTGTCGAAAGAAAATTGTGCAGCTTTACACACGAGCATCAAAGAGGTCCTTGAAAAATCCCTTGAAGTTGGAGCAGATAGTAGCCAATTTCCTAGTGATTGGATTTTTCATTCCCGTGAAAAGAAGCCTGGAAAGGCTTTTGTTGATGGGAAGAAAATTGAGTTTATCACGGTTGGTAGCAGGACTTCAGCATTTGTACCAGAGCTTCAGAAGTTAAGTGGACCGCAAGCTTCCAAAGCAGCAAGTAAACAAGCTAACAAGAAAAAAGGGCGTGGTGATGTTGTTAACGATGATGTTGATGAAGCAGAAAGTGATGAAGAAGCCAATGAAAAAGAAAAATCGAAGAAAGGGAGGAAGCCTAAAGGTCAAGGTAAGAGGGCGTCGGGGAAAAGAAAATCAAAAGAGAGTGATGATGAAGACAAGGACAGTGATGATGATGATGCTGACGACGATGACCATCAGGATGAAGATCAAAAGAAGAAAACAAGAAAAGGTGTCAATAATAAGCAATCCAAGTCACAGAAGACATCAAAGGCAAAGGTGCCGACCAGTCAAAGTGGTAAGAAGCTGAAGAAAGGAAAATAG

Protein Analysis

401

Amino Acids

44.22

Weight (kDa)

8.95

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 1.7e-28 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 236 2.3e-31 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 240 - 273 4.2e-22 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 1159
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 3 cut(s) 475, 789, 809
AcyI GRCGYC 1 cut(s) 998
AfaI GTAC 2 cut(s) 576, 816
AfiI CCNNNNNNNGG 2 cut(s) 172, 306
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 4 cut(s) 338, 485, 671, 683
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AluBI AGCT 6 cut(s) 10, 641, 823, 848, 872, 1186
AluI AGCT 6 cut(s) 10, 641, 823, 848, 872, 1186
Alw26I GTCTC 2 cut(s) 118, 163
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 4 cut(s) 36, 132, 308, 440
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 3 cut(s) 611, 638, 857
ApoI RAATTY 1 cut(s) 591
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 5 cut(s) 36, 440, 441, 664, 839
AsuHPI GGTGA 1 cut(s) 905
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 664, 839
BaeGI GKGCMC 2 cut(s) 423, 444
BanI GGYRCC 1 cut(s) 1159
BanII GRGCYC 1 cut(s) 444
BarI GAAGNNNNNNTAC 4 cut(s) 787, 807, 819, 839
BauI CACGAG 1 cut(s) 649
BbsI GAAGAC 2 cut(s) 1041, 1148
BbvI GCAGC 3 cut(s) 598, 650, 869
BccI CCATC 5 cut(s) 101, 161, 341, 758, 1083
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 747
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 118, 163
BfaI CTAG 2 cut(s) 387, 711
BisI GCNGC 4 cut(s) 27, 612, 639, 858
BlsI GCNGC 4 cut(s) 28, 613, 640, 859
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 2 cut(s) 664, 839
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 5 cut(s) 36, 440, 441, 664, 839
BmiI GGNNCC 3 cut(s) 442, 459, 1161
BmrFI CCNGG 1 cut(s) 747
BmsI GCATC 3 cut(s) 436, 663, 1048
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 1041, 1148
BpmI CTGGAG 1 cut(s) 374
Bsa29I ATCGAT 1 cut(s) 105
BsaBI GATNNNNATC 2 cut(s) 1074, 1086
BsaHI GRCGYC 1 cut(s) 998
BsaWI WCCGGW 2 cut(s) 13, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 306
Bse1I ACTGG 1 cut(s) 1167
Bse8I GATNNNNATC 2 cut(s) 1074, 1086
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 1 cut(s) 747
BseCI ATCGAT 1 cut(s) 105
BseGI GGATG 3 cut(s) 349, 595, 1087
BseJI GATNNNNATC 2 cut(s) 1074, 1086
BseLI CCNNNNNNNGG 2 cut(s) 172, 306
BseNI ACTGG 1 cut(s) 1167
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 423, 444
BseXI GCAGC 3 cut(s) 598, 650, 869
BsgI GTGCAG 1 cut(s) 657
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 38, 134, 310, 442
BshNI GGYRCC 1 cut(s) 1159
BshVI ATCGAT 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 3 cut(s) 5, 14, 599
BslI CCNNNNNNNGG 2 cut(s) 172, 306
BsmAI GTCTC 2 cut(s) 118, 163
BsmBI CGTCTC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 4 cut(s) 38, 134, 310, 442
BsoBI CYCGRG 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 423, 444
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 3 cut(s) 406, 529, 1087
BspACI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
BspANI GGCC 4 cut(s) 38, 134, 310, 442
BspDI ATCGAT 1 cut(s) 105
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 442, 459, 1161
BspPI GGATC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 1159
BsrI ACTGG 1 cut(s) 1167
BssMI GATC 3 cut(s) 406, 529, 1087
BssNI GRCGYC 1 cut(s) 998
BssSI CACGAG 1 cut(s) 649
Bst2BI CACGAG 1 cut(s) 649
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 3 cut(s) 469, 790, 1046
BstACI GRCGYC 1 cut(s) 998
BstC8I GCNNGC 3 cut(s) 398, 616, 846
BstF5I GGATG 3 cut(s) 349, 595, 1087
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 3 cut(s) 409, 532, 1090
BstMAI GTCTC 2 cut(s) 118, 163
BstMBI GATC 3 cut(s) 406, 529, 1087
BstMWI GCNNNNNNNGC 3 cut(s) 35, 83, 854
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 155
BstSCI CCNGG 1 cut(s) 745
BstSLI GKGCMC 2 cut(s) 423, 444
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 3 cut(s) 598, 650, 869
BstV2I GAAGAC 2 cut(s) 1041, 1148
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 105
BsuRI GGCC 4 cut(s) 38, 134, 310, 442
BsuTUI ATCGAT 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 349, 595, 1087
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 4 cut(s) 49, 366, 474, 1051
Cac8I GCNNGC 3 cut(s) 398, 616, 846
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 5 cut(s) 36, 440, 441, 664, 839
ClaI ATCGAT 1 cut(s) 105
CseI GACGC 1 cut(s) 987
Csp6I GTAC 2 cut(s) 575, 815
CviAII CATG 1 cut(s) 184
CviQI GTAC 2 cut(s) 575, 815
DpnI GATC 3 cut(s) 408, 531, 1089
DpnII GATC 3 cut(s) 406, 529, 1087
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 2 cut(s) 664, 839
Eco57I CTGAAG 3 cut(s) 475, 789, 809
Eco88I CYCGRG 1 cut(s) 437
EcoO109I RGGNCCY 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 1 cut(s) 187
FaiI YATR 7 cut(s) 41, 185, 247, 249, 276, 428, 464
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 4 cut(s) 27, 612, 639, 858
FokI GGATG 3 cut(s) 356, 582, 1094
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 4 cut(s) 27, 612, 639, 858
FspBI CTAG 2 cut(s) 387, 711
GlaI GCGC 1 cut(s) 121
GluI GCNGC 4 cut(s) 27, 612, 639, 858
GsuI CTGGAG 1 cut(s) 374
HaeIII GGCC 4 cut(s) 38, 134, 310, 442
HapII CCGG 3 cut(s) 5, 14, 599
HgaI GACGC 1 cut(s) 987
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 998
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 846
HinfI GANTC 4 cut(s) 92, 111, 200, 390
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 3 cut(s) 5, 14, 599
HphI GGTGA 1 cut(s) 905
Hpy166II GTNNAC 3 cut(s) 839, 866, 904
Hpy188I TCNGA 5 cut(s) 33, 230, 433, 534, 828
Hpy188III TCNNGA 5 cut(s) 268, 353, 546, 599, 1079
Hpy8I GTNNAC 3 cut(s) 839, 866, 904
Hpy99I CGWCG 3 cut(s) 92, 1003, 1070
HpyAV CCTTC 3 cut(s) 225, 229, 321
HpyCH4III ACNGT 3 cut(s) 469, 790, 1046
HpyCH4V TGCA 1 cut(s) 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 83, 854
Hsp92I GRCGYC 1 cut(s) 998
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 3 cut(s) 406, 529, 1087
LmnI GCTCC 4 cut(s) 7, 457, 601, 689
Lsp1109I GCAGC 3 cut(s) 598, 650, 869
LweI GCATC 3 cut(s) 436, 663, 1048
MaeI CTAG 2 cut(s) 387, 711
MaeIII GTNAC 2 cut(s) 265, 1134
MalI GATC 3 cut(s) 408, 531, 1089
MboI GATC 3 cut(s) 406, 529, 1087
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 423, 444
MluCI AATT 9 cut(s) 224, 338, 504, 549, 556, 591, 631, 704, 774
MlyI GAGTC 4 cut(s) 86, 120, 194, 399
MmeI TCCRAC 2 cut(s) 434, 667
MroI TCCGGA 1 cut(s) 598
MseI TTAA 4 cut(s) 252, 513, 833, 903
MslI CAYNNNNRTG 2 cut(s) 732, 1080
MspI CCGG 3 cut(s) 5, 14, 599
MspR9I CCNGG 1 cut(s) 747
MunI CAATTG 1 cut(s) 556
Mva1269I GAATGC 1 cut(s) 491
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 35, 83, 854
NdeII GATC 3 cut(s) 406, 529, 1087
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 3 cut(s) 442, 459, 1161
NmuCI GTSAC 2 cut(s) 265, 1134
NspI RCATGY 1 cut(s) 187
PceI AGGCCT 1 cut(s) 134
PciI ACATGT 1 cut(s) 183
PctI GAATGC 1 cut(s) 491
PkrI GCNGC 4 cut(s) 28, 613, 640, 859
PleI GAGTC 4 cut(s) 86, 119, 194, 398
PpsI GAGTC 4 cut(s) 86, 119, 194, 398
PpuMI RGGWCCY 1 cut(s) 664
PscI ACATGT 1 cut(s) 183
PshAI GACNNNNGTC 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 3 cut(s) 442, 459, 1161
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 5 cut(s) 36, 440, 441, 664, 839
PspPPI RGGWCCY 1 cut(s) 664
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 2 cut(s) 576, 816
RsaNI GTAC 2 cut(s) 575, 815
RseI CAYNNNNRTG 2 cut(s) 732, 1080
SaqAI TTAA 4 cut(s) 252, 513, 833, 903
SatI GCNGC 4 cut(s) 27, 612, 639, 858
Sau3AI GATC 3 cut(s) 406, 529, 1087
Sau96I GGNCC 5 cut(s) 36, 440, 441, 664, 839
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 4 cut(s) 86, 120, 194, 399
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 2 cut(s) 423, 444
SfaNI GCATC 3 cut(s) 436, 663, 1048
SinI GGWCC 2 cut(s) 664, 839
SmiMI CAYNNNNRTG 2 cut(s) 732, 1080
Sse9I AATT 9 cut(s) 224, 338, 504, 549, 556, 591, 631, 704, 774
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 6 cut(s) 26, 162, 169, 236, 394, 842
SspMI CTAG 2 cut(s) 387, 711
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 745
TaaI ACNGT 3 cut(s) 469, 790, 1046
TaqI TCGA 5 cut(s) 105, 129, 198, 623, 959
TasI AATT 9 cut(s) 224, 338, 504, 549, 556, 591, 631, 704, 774
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 252, 513, 833, 903
Tru9I TTAA 4 cut(s) 252, 513, 833, 903
TscAI CASTG 4 cut(s) 56, 373, 474, 1051
TseFI GTSAC 2 cut(s) 265, 1134
TseI GCWGC 3 cut(s) 611, 638, 857
Tsp45I GTSAC 2 cut(s) 265, 1134
TspDTI ATGAA 9 cut(s) 489, 584, 585, 716, 933, 948, 960, 1047, 1098
TspRI CASTG 4 cut(s) 56, 373, 474, 1051
VpaK11BI GGWCC 2 cut(s) 664, 839
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 187
XspI CTAG 2 cut(s) 387, 711
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.