Rh2DG394900

Formamidopyrimidine-DNA glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005648
Physical Location & Seq
Forward (+)
57447844 .. 57451377
3534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG394900.1

Sequence Viewer

Length: 1197 bp
ATGCCAGAGCTACCGGAGGTGGAGGCGGCCCGGAGGGCCATAGAGGAAAACTGCGTCGGGAAGAAGATCACGAAAGCAATCGTAGCCGACGACTCCAAAGTCATCGACGGAGTCTCGCGCGCGGACTTCGAGGCCTCGCTCTTGGGGAAGACCATCGTCGCCGCCCACCGCAAGGGCAAGAACATGTGGCTCCAACTTGACTCTCCTCCTTTCCCTTCCTTCCAATTCGGGATGGCGGGTGCTGTGTATATTAAAGGGGTTGCGGTCACCAAGTACAAGAGGTCTGCTGTGAAGGACACTGATGAGTGGCCTTCCAAATATTCCAAGGTTTTTGTCCAACTGGATGATGGTCTGGAGTTCTCGTTCACTGACAAGAGGCGATTTGCAAGAGTCCGCCTACTTGAAGATCCAACTTCTGTGCCCCCCATATCTGAGCTCGGGCCCGATGCTCTCTTGGAGCCAATGACAGTAGATGAACTTCTTGCATCATTAAGCAAGAAGAAAATATCAATTAAGGCTCTATTGCTTGATCAGAGCTTTATATCAGGAATTGGCAATTGGGTTGCTGATGAAGTACTATACCAAGCAAGAATTCATCCGGAGCAAAGTTCTGCCACCCTGTCCAAAGAATGTGTTGGAGCCTTACACAAAAGCATCAAAGAGGTCATTGAAAAATCAGTTGAAGTTGGAGCAGATAGTGATCAATATCCTAGTAATTGGATTTTTCATTCCCGTGAAAAGAAACCGGGAAAGGTGTTGGTTGATGGGAAGAAGATTAATTTTATCACAGTTGGTGGCAGGACTTCAGCTTTTGTACCAGAGCTTCAGAAGTTAACTGGAAATCAAGCTGCCAAAGCAGGAAGTAAACGAACTAATAAGAGTATTGATGATGTCAATGAAGAAGCAAGTGATGAAGAAGTCAAGGGAACTGCCAAATCTAAGAAAGGTAGGAAGTCTGGAGGTCAGGCCAAGAGGTCTGCCGTGAAAAGAAAATCTAAAGACAGTGATGATGAAGACAATGACAATGATGATTCTGCTGATGATGTCGATCATGAAGACAGTGATGATGATGCTGATGAAGATCGAAAGAACAAAACAAGAAAGGTGACAACTAGTAAGCAATCCAAGGCAGGGACGACATCAAAGAAAAAGGTGCAGACCAGTCAAAATAGTAAACCAAAGAAGAGAGCGAAGTAG

Protein Analysis

398

Amino Acids

43.74

Weight (kDa)

8.98

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fapy_DNA_glyco PF01149 1 - 131 5.7e-29 Formamidopyrimidine-DNA glycosylase N-terminal domain
H2TH PF06831 146 - 233 1.1e-28 Formamidopyrimidine-DNA glycosylase H2TH domain
Fpg-like_C PF21218 240 - 273 9e-21 Formamidopyrimidine-DNA glycosylase-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccII CGCG 3 cut(s) 118, 120, 122
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 789, 809
AfaI GTAC 3 cut(s) 275, 576, 816
AfiI CCNNNNNNNGG 2 cut(s) 172, 1131
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 3 cut(s) 404, 671, 683
AhlI ACTAGT 1 cut(s) 1112
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 576, 608
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 10, 436, 537, 809, 823, 848
AluI AGCT 6 cut(s) 10, 436, 537, 809, 823, 848
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 598
AoxI GGCC 6 cut(s) 27, 36, 132, 308, 440, 966
ApaI GGGCCC 1 cut(s) 444
ApeKI GCWGC 1 cut(s) 848
ApoI RAATTY 1 cut(s) 591
AseI ATTAAT 1 cut(s) 777
AspLEI GCGC 2 cut(s) 120, 122
AspS9I GGNCC 4 cut(s) 28, 36, 440, 441
AsuC2I CCSGG 2 cut(s) 31, 747
AsuHPI GGTGA 2 cut(s) 259, 1117
AvaI CYCGRG 1 cut(s) 437
BaeGI GKGCMC 2 cut(s) 423, 444
BanII GRGCYC 2 cut(s) 438, 444
BarI GAAGNNNNNNTAC 4 cut(s) 462, 494, 807, 839
BbsI GAAGAC 3 cut(s) 155, 1020, 1062
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 1 cut(s) 835
BccI CCATC 4 cut(s) 161, 226, 341, 758
BceAI ACGGC 1 cut(s) 965
BclI TGATCA 2 cut(s) 529, 700
BcnI CCSGG 2 cut(s) 31, 747
BcoDI GTCTC 1 cut(s) 118
BcuI ACTAGT 1 cut(s) 1112
BfaI CTAG 2 cut(s) 711, 1113
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 3 cut(s) 27, 162, 849
BlsI GCNGC 3 cut(s) 28, 163, 850
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 2 cut(s) 31, 747
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 4 cut(s) 28, 36, 440, 441
BmiI GGNNCC 4 cut(s) 191, 442, 459, 640
BmrFI CCNGG 2 cut(s) 31, 747
BmsI GCATC 4 cut(s) 436, 494, 663, 1060
BoxI GACNNNNGTC 1 cut(s) 155
BpiI GAAGAC 3 cut(s) 155, 1020, 1062
BpmI CTGGAG 2 cut(s) 374, 978
BpuMI CCSGG 2 cut(s) 31, 747
BsaBI GATNNNNATC 4 cut(s) 699, 705, 1047, 1080
BsaJI CCNNGG 2 cut(s) 324, 1125
BsaWI WCCGGW 2 cut(s) 13, 598
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 1131
Bse1I ACTGG 3 cut(s) 345, 841, 1161
Bse8I GATNNNNATC 4 cut(s) 699, 705, 1047, 1080
BseAI TCCGGA 1 cut(s) 598
BseDI CCNNGG 2 cut(s) 324, 1125
BseGI GGATG 3 cut(s) 237, 349, 595
BseJI GATNNNNATC 4 cut(s) 699, 705, 1047, 1080
BseLI CCNNNNNNNGG 2 cut(s) 172, 1131
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 345, 841, 1161
BsePI GCGCGC 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 195
BseSI GKGCMC 2 cut(s) 423, 444
BseXI GCAGC 1 cut(s) 835
BsgI GTGCAG 1 cut(s) 1175
Bsh1236I CGCG 3 cut(s) 118, 120, 122
BshFI GGCC 6 cut(s) 29, 38, 134, 310, 442, 968
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 4 cut(s) 14, 31, 599, 746
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 2 cut(s) 172, 1131
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 1147
BsnI GGCC 6 cut(s) 29, 38, 134, 310, 442, 968
BsoBI CYCGRG 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 423, 438, 444
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
BspACI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
BspANI GGCC 6 cut(s) 29, 38, 134, 310, 442, 968
BspCNI CTCAG 1 cut(s) 424
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 3 cut(s) 118, 120, 122
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 4 cut(s) 191, 442, 459, 640
BspPI GGATC 1 cut(s) 401
BsrI ACTGG 3 cut(s) 345, 841, 1161
BssECI CCNNGG 2 cut(s) 324, 1125
BssHII GCGCGC 1 cut(s) 118
BssMI GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
BssT1I CCWWGG 2 cut(s) 324, 1125
Bst4CI ACNGT 4 cut(s) 469, 790, 1004, 1061
Bst6I CTCTTC 1 cut(s) 1178
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 432, 939
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 3 cut(s) 237, 349, 595
BstFNI CGCG 3 cut(s) 118, 120, 122
BstHHI GCGC 2 cut(s) 120, 122
BstKTI GATC 6 cut(s) 69, 409, 532, 703, 1051, 1084
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 35, 83, 854
BstNSI RCATGY 1 cut(s) 187
BstPAI GACNNNNGTC 1 cut(s) 155
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 29, 745
BstSLI GKGCMC 2 cut(s) 423, 444
BstUI CGCG 3 cut(s) 118, 120, 122
BstV1I GCAGC 1 cut(s) 835
BstV2I GAAGAC 3 cut(s) 155, 1020, 1062
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 6 cut(s) 29, 38, 134, 310, 442, 968
BtsCI GGATG 3 cut(s) 237, 349, 595
BtsIMutI CAGTG 4 cut(s) 297, 366, 1009, 1066
Cac8I GCNNGC 1 cut(s) 120
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 2 cut(s) 120, 122
Cfr13I GGNCC 4 cut(s) 28, 36, 440, 441
CseI GACGC 1 cut(s) 43
Csp6I GTAC 3 cut(s) 274, 575, 815
CviAII CATG 2 cut(s) 184, 1052
CviQI GTAC 3 cut(s) 274, 575, 815
DdeI CTNAG 2 cut(s) 432, 939
DpnI GATC 6 cut(s) 68, 408, 531, 702, 1050, 1083
DpnII GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 1178
EarI CTCTTC 1 cut(s) 1178
EciI GGCGGA 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 324, 1125
Eco147I AGGCCT 1 cut(s) 134
Eco24I GRGCYC 2 cut(s) 438, 444
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 789, 809
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 591
EcoT14I CCWWGG 2 cut(s) 324, 1125
EcoT38I GRGCYC 2 cut(s) 438, 444
ErhI CCWWGG 2 cut(s) 324, 1125
FaeI CATG 2 cut(s) 187, 1055
FaiI YATR 7 cut(s) 41, 185, 249, 428, 542, 580, 1053
FaqI GGGAC 1 cut(s) 1147
FatI CATG 2 cut(s) 183, 1051
FauI CCCGC 1 cut(s) 229
FbaI TGATCA 2 cut(s) 529, 700
Fnu4HI GCNGC 3 cut(s) 27, 162, 849
FokI GGATG 3 cut(s) 244, 356, 582
FriOI GRGCYC 2 cut(s) 438, 444
Fsp4HI GCNGC 3 cut(s) 27, 162, 849
FspBI CTAG 2 cut(s) 711, 1113
GlaI GCGC 2 cut(s) 119, 121
GluI GCNGC 3 cut(s) 27, 162, 849
GsuI CTGGAG 2 cut(s) 374, 978
HaeIII GGCC 6 cut(s) 29, 38, 134, 310, 442, 968
HapII CCGG 4 cut(s) 14, 31, 599, 746
HgaI GACGC 1 cut(s) 43
HhaI GCGC 2 cut(s) 120, 122
Hin1II CATG 2 cut(s) 187, 1055
Hin6I GCGC 2 cut(s) 118, 120
HinP1I GCGC 2 cut(s) 118, 120
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HinfI GANTC 5 cut(s) 92, 111, 200, 390, 1031
HpaI GTTAAC 1 cut(s) 834
HpaII CCGG 4 cut(s) 14, 31, 599, 746
HphI GGTGA 2 cut(s) 259, 1117
Hpy166II GTNNAC 4 cut(s) 366, 834, 866, 1175
Hpy188I TCNGA 3 cut(s) 433, 534, 828
Hpy188III TCNNGA 8 cut(s) 58, 70, 229, 353, 546, 599, 957, 1052
Hpy8I GTNNAC 4 cut(s) 366, 834, 866, 1175
Hpy99I CGWCG 4 cut(s) 59, 92, 110, 161
HpyAV CCTTC 4 cut(s) 225, 229, 286, 321
HpyCH4III ACNGT 4 cut(s) 469, 790, 1004, 1061
HpyCH4V TGCA 3 cut(s) 386, 485, 1156
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 83, 854
HpyF3I CTNAG 2 cut(s) 432, 939
Hsp92II CATG 2 cut(s) 187, 1055
HspAI GCGC 2 cut(s) 118, 120
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 2 cut(s) 529, 700
KspAI GTTAAC 1 cut(s) 834
Kzo9I GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
LmnI GCTCC 5 cut(s) 195, 457, 601, 638, 689
Lsp1109I GCAGC 1 cut(s) 835
LweI GCATC 4 cut(s) 436, 494, 663, 1060
MaeI CTAG 2 cut(s) 711, 1113
MaeIII GTNAC 2 cut(s) 265, 1105
MalI GATC 6 cut(s) 68, 408, 531, 702, 1050, 1083
MauBI CGCGCGCG 1 cut(s) 118
MboI GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 3 cut(s) 423, 438, 444
MluCI AATT 7 cut(s) 224, 510, 549, 556, 591, 715, 778
MlyI GAGTC 4 cut(s) 86, 120, 194, 399
MmeI TCCRAC 5 cut(s) 217, 361, 434, 616, 667
MroI TCCGGA 1 cut(s) 598
MseI TTAA 5 cut(s) 252, 491, 513, 777, 833
MslI CAYNNNNRTG 1 cut(s) 732
MspI CCGG 4 cut(s) 14, 31, 599, 746
MspR9I CCNGG 2 cut(s) 31, 747
MunI CAATTG 1 cut(s) 556
MvnI CGCG 3 cut(s) 118, 120, 122
MwoI GCNNNNNNNGC 3 cut(s) 35, 83, 854
NciI CCSGG 2 cut(s) 31, 747
NdeII GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
NlaIII CATG 2 cut(s) 187, 1055
NlaIV GGNNCC 4 cut(s) 191, 442, 459, 640
NmuCI GTSAC 2 cut(s) 265, 1105
NspI RCATGY 1 cut(s) 187
PagI TCATGA 1 cut(s) 1051
PauI GCGCGC 1 cut(s) 118
PceI AGGCCT 1 cut(s) 134
PciI ACATGT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 1 cut(s) 1031
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 28, 163, 850
PleI GAGTC 4 cut(s) 86, 119, 194, 398
PpsI GAGTC 4 cut(s) 86, 119, 194, 398
PscI ACATGT 1 cut(s) 183
PshAI GACNNNNGTC 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 777
Psp124BI GAGCTC 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 265
PspN4I GGNNCC 4 cut(s) 191, 442, 459, 640
PspOMI GGGCCC 1 cut(s) 440
PspPI GGNCC 4 cut(s) 28, 36, 440, 441
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 1 cut(s) 110
PteI GCGCGC 1 cut(s) 118
RsaI GTAC 3 cut(s) 275, 576, 816
RsaNI GTAC 3 cut(s) 274, 575, 815
RseI CAYNNNNRTG 1 cut(s) 732
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 5 cut(s) 252, 491, 513, 777, 833
SatI GCNGC 3 cut(s) 27, 162, 849
Sau3AI GATC 6 cut(s) 66, 406, 529, 700, 1048, 1081
Sau96I GGNCC 4 cut(s) 28, 36, 440, 441
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 4 cut(s) 86, 120, 194, 399
ScrFI CCNGG 2 cut(s) 31, 747
SduI GDGCHC 3 cut(s) 423, 438, 444
SfaNI GCATC 4 cut(s) 436, 494, 663, 1060
SfiI GGCCNNNNNGGCC 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 732
SpeI ACTAGT 1 cut(s) 1112
Sse9I AATT 7 cut(s) 224, 510, 549, 556, 591, 715, 778
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 7 cut(s) 26, 122, 162, 169, 236, 263, 394
SspI AATATT 1 cut(s) 320
SspMI CTAG 2 cut(s) 711, 1113
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 29, 745
StyI CCWWGG 2 cut(s) 324, 1125
TaaI ACNGT 4 cut(s) 469, 790, 1004, 1061
TaqI TCGA 4 cut(s) 105, 129, 1047, 1084
TasI AATT 7 cut(s) 224, 510, 549, 556, 591, 715, 778
TatI WGTACW 2 cut(s) 273, 574
TauI GCSGC 2 cut(s) 29, 164
TfiI GAWTC 1 cut(s) 1031
Tru1I TTAA 5 cut(s) 252, 491, 513, 777, 833
Tru9I TTAA 5 cut(s) 252, 491, 513, 777, 833
TscAI CASTG 4 cut(s) 304, 373, 1009, 1066
TseFI GTSAC 2 cut(s) 265, 1105
TseI GCWGC 1 cut(s) 848
Tsp45I GTSAC 2 cut(s) 265, 1105
TspDTI ATGAA 9 cut(s) 489, 584, 585, 716, 912, 927, 1026, 1068, 1092
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 4 cut(s) 304, 373, 1009, 1066
Tth111I GACNNNGTC 1 cut(s) 110
VspI ATTAAT 1 cut(s) 777
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XspI CTAG 2 cut(s) 711, 1113
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.