AT1G53100

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007023
Physical Location & Seq
Reverse (-)
19786900 .. 19789150
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53100.2

Sequence Viewer

Length: 1272 bp
ATGCTTTTGTCTCAAGATCACAAAATTCTCTTCAATGGGGTACGTAAATATGTAATCATGGAGAAGAAATATGTATTTTCATTTGTTATCACATCTCTTGTCTGCGTTGTTCTCCTTGCAACTTCCTTCAACATTGGTTTAATGTCTTCACTTCGACCACCGATTAATGGAACTTTATCAAGTTTCCCCAAAAATGATTCGAACGTTGTAGGAAAACAACCTCGTGAAGACGACAAGCTCCCACGTTTCGCTTACCTTGTTTCAGGATCTAAAGGAGATCTTGAAAGCCTTTGGAGAACTCTTAGAGCATTGTACCATCCAAGAAACCAATACATTGTTCACTTGGATCTTGAATCACCGGTTGATGAGAGATCAGAGTTAGCTTCTCGCATTTCAAACGATCCTATGTATTCAAAAGCAGGAAATGTATATATGATAACTAAAGCTAATCTTGTGACTTATACCGGACCAACAATGGTGGCGAATACGCTTCACGCATGTGCGATTCTTCTTAAAAGAACCCCGGATTGGGATTGGTTTATCAATCTTAGTGCTTCGGATTATCCCCTCGTGACTCAAGATGATCTGATTCATACATTTTCGACATTGGACCGGAACCTTAACTTTATCGAGCACACAAGTTCCTTAGGCTGGAAATACAAGAAGCGGGCAATGCCATTAATAATTGATCCTGGACTCTATATGTTAAACAAATCAAACGTTTTATTGGTTAGACCTAATCGAAGTTTACCTTCTGCTTTCAAGTTATTTACCGCTTGGATGGCTCTATCGCACGCATTCGTGGAGTATATCATATACGGTTGGGACAATTTACCAAGAACTTTACTCATGTACTACACCAATTTCGTTTCTTCTCCTGAAGGTTACTTCCATACAGTCATATGTAACGTGCCTGAGTTCTCCAAAACCGCGGTGAACCATGATCTTCACTACATCGCATGGGACAAGCCACCGAGGCAGCATCCTCGTATGTTGTCTCAAAGAGACATGGGAAAGATGATTTCGAGCGGATCTGCATTTGGTCGGAAGTTCAGAAGAAATGATACTGTTTTGGATAAGATTGATAAGGAACTGCTTATACGGATAAACGAAGACGGTTTTACTCCAGGCGGTTGGTGCGGTGGGAAACCAGAGTGCTCGGTTGTCGAAGATGTGGCTAGGATCAGGCCTAGCTCAGGGGCTGTGAGGCTCAAGGAGCTTGTAGATAGGTTAGTTACAGAGGCTAAATTAGGTAAGAATCAATGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

423

Amino Acids

48.17

Weight (kDa)

9.23

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 83 - 340 3.7e-78 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1029
AccII CGCG 1 cut(s) 932
AciI CCGC 7 cut(s) 667, 774, 930, 932, 1029, 1131, 1140
AclI AACGTT 2 cut(s) 204, 720
AclWI GGATC 6 cut(s) 274, 354, 395, 683, 1039, 1190
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 900
AfaI GTAC 3 cut(s) 42, 314, 854
AfiI CCNNNNNNNGG 5 cut(s) 167, 528, 529, 651, 1196
AgeI ACCGGT 1 cut(s) 358
AgsI TTSAA 7 cut(s) 34, 130, 284, 353, 396, 414, 763
AjnI CCWGG 2 cut(s) 691, 1126
AjuI GAANNNNNNNTTGG 4 cut(s) 321, 353, 511, 543
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 5 cut(s) 238, 383, 446, 1194, 1219
AluI AGCT 5 cut(s) 238, 383, 446, 1194, 1219
Alw21I GWGCWC 2 cut(s) 636, 1160
Alw26I GTCTC 3 cut(s) 15, 999, 1002
AlwI GGATC 6 cut(s) 274, 354, 395, 683, 1039, 1190
AlwNI CAGNNNCTG 1 cut(s) 1202
AoxI GGCC 1 cut(s) 1187
ApeKI GCWGC 1 cut(s) 979
ApoI RAATTY 1 cut(s) 24
AseI ATTAAT 2 cut(s) 165, 680
AsiGI ACCGGT 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 467, 610
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 348, 946
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 467, 610
AxyI CCTNAGG 1 cut(s) 646
BarI GAAGNNNNNNTAC 2 cut(s) 1048, 1080
BauI CACGAG 2 cut(s) 222, 569
BbsI GAAGAC 3 cut(s) 138, 234, 1119
Bbv12I GWGCWC 2 cut(s) 636, 1160
BbvI GCAGC 1 cut(s) 991
BccI CCATC 2 cut(s) 324, 775
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BciT130I CCWGG 2 cut(s) 693, 1128
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 3 cut(s) 15, 999, 1002
BfaI CTAG 2 cut(s) 1179, 1191
BglI GCCNNNNNGGC 1 cut(s) 976
BglII AGATCT 1 cut(s) 277
BisI GCNGC 1 cut(s) 980
BlsI GCNGC 1 cut(s) 981
Bme1390I CCNGG 3 cut(s) 524, 693, 1128
Bme18I GGWCC 2 cut(s) 467, 610
BmgT120I GGNCC 2 cut(s) 467, 610
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 3 cut(s) 524, 693, 1128
BmsI GCATC 1 cut(s) 991
BpiI GAAGAC 3 cut(s) 138, 234, 1119
BpmI CTGGAG 1 cut(s) 1110
Bpu10I CCTNAGC 1 cut(s) 1195
Bpu14I TTCGAA 1 cut(s) 200
BpuEI CTTGAG 2 cut(s) 561, 1196
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 522, 930, 974
BsaWI WCCGGW 3 cut(s) 358, 464, 612
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 528, 529, 651, 1196
Bse118I RCCGGY 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 646
Bse3DI GCAATG 1 cut(s) 678
BseBI CCWGG 2 cut(s) 693, 1128
BseDI CCNNGG 3 cut(s) 522, 930, 974
BseGI GGATG 3 cut(s) 316, 786, 982
BseLI CCNNNNNNNGG 5 cut(s) 167, 528, 529, 651, 1196
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 906, 1209
BseXI GCAGC 1 cut(s) 991
Bsh1236I CGCG 1 cut(s) 932
BshFI GGCC 1 cut(s) 1189
BshTI ACCGGT 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 636, 1160
BsiSI CCGG 4 cut(s) 359, 465, 524, 613
BslFI GGGAC 2 cut(s) 839, 977
BslI CCNNNNNNNGG 5 cut(s) 167, 528, 529, 651, 1196
BsmAI GTCTC 3 cut(s) 15, 999, 1002
BsmFI GGGAC 2 cut(s) 839, 977
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 1 cut(s) 1189
Bsp119I TTCGAA 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 636, 1160
BspACI CCGC 7 cut(s) 667, 774, 930, 932, 1029, 1131, 1140
BspANI GGCC 1 cut(s) 1189
BspCNI CTCAG 2 cut(s) 907, 1208
BspFNI CGCG 1 cut(s) 932
BspLI GGNNCC 1 cut(s) 617
BspPI GGATC 6 cut(s) 274, 354, 395, 683, 1039, 1190
BspT104I TTCGAA 1 cut(s) 200
BsrBI CCGCTC 1 cut(s) 1029
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 3 cut(s) 522, 930, 974
BssSI CACGAG 2 cut(s) 222, 569
Bst2BI CACGAG 2 cut(s) 222, 569
Bst2UI CCWGG 2 cut(s) 693, 1128
Bst4CI ACNGT 4 cut(s) 821, 898, 1069, 1118
Bst6I CTCTTC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 669, 795
BstDEI CTNAG 5 cut(s) 302, 548, 646, 915, 1195
BstDSI CCRYGG 1 cut(s) 930
BstENI CCTNNNNNAGG 1 cut(s) 1194
BstF5I GGATG 3 cut(s) 316, 786, 982
BstFNI CGCG 1 cut(s) 932
BstMAI GTCTC 3 cut(s) 15, 999, 1002
BstMWI GCNNNNNNNGC 5 cut(s) 673, 782, 976, 1137, 1216
BstNI CCWGG 2 cut(s) 693, 1128
BstNSI RCATGY 1 cut(s) 501
BstSCI CCNGG 3 cut(s) 522, 691, 1126
BstSNI TACGTA 1 cut(s) 44
BstUI CGCG 1 cut(s) 932
BstV1I GCAGC 1 cut(s) 991
BstV2I GAAGAC 3 cut(s) 138, 234, 1119
BstX2I RGATCY 4 cut(s) 266, 277, 346, 1031
BstXI CCANNNNNNTGG 1 cut(s) 1134
BstYI RGATCY 4 cut(s) 266, 277, 346, 1031
Bsu36I CCTNAGG 1 cut(s) 646
BsuRI GGCC 1 cut(s) 1189
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 940
BtsCI GGATG 3 cut(s) 316, 786, 982
Cac8I GCNNGC 2 cut(s) 669, 795
CaiI CAGNNNCTG 1 cut(s) 1202
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 467, 610
Cfr42I CCGCGG 1 cut(s) 933
Csp6I GTAC 3 cut(s) 41, 313, 853
CspAI ACCGGT 1 cut(s) 358
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 6 cut(s) 58, 498, 850, 941, 960, 1009
CviQI GTAC 3 cut(s) 41, 313, 853
DdeI CTNAG 5 cut(s) 302, 548, 646, 915, 1195
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco105I TACGTA 1 cut(s) 44
Eco147I AGGCCT 1 cut(s) 1189
Eco47I GGWCC 2 cut(s) 467, 610
Eco57I CTGAAG 1 cut(s) 900
Eco81I CCTNAGG 1 cut(s) 646
EcoNI CCTNNNNNAGG 1 cut(s) 1194
EcoRII CCWGG 2 cut(s) 691, 1126
FaeI CATG 6 cut(s) 61, 501, 853, 944, 963, 1012
FalI AAGNNNNNCTT 6 cut(s) 264, 296, 435, 467, 736, 768
FaqI GGGAC 2 cut(s) 839, 977
FatI CATG 6 cut(s) 57, 497, 849, 940, 959, 1008
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 902
Fnu4HI GCNGC 1 cut(s) 980
FokI GGATG 3 cut(s) 303, 793, 969
Fsp4HI GCNGC 1 cut(s) 980
FspBI CTAG 2 cut(s) 1179, 1191
GluI GCNGC 1 cut(s) 980
GsuI CTGGAG 1 cut(s) 1110
HaeIII GGCC 1 cut(s) 1189
HapII CCGG 4 cut(s) 359, 465, 524, 613
Hin1II CATG 6 cut(s) 61, 501, 853, 944, 963, 1012
HinfI GANTC 7 cut(s) 197, 353, 505, 574, 589, 696, 1258
HpaII CCGG 4 cut(s) 359, 465, 524, 613
HphI GGTGA 2 cut(s) 348, 946
Hpy166II GTNNAC 3 cut(s) 340, 749, 937
Hpy188I TCNGA 5 cut(s) 376, 559, 588, 1047, 1055
Hpy188III TCNNGA 8 cut(s) 14, 224, 264, 281, 350, 571, 578, 878
Hpy8I GTNNAC 3 cut(s) 340, 749, 937
HpyAV CCTTC 3 cut(s) 136, 762, 875
HpyCH4III ACNGT 4 cut(s) 821, 898, 1069, 1118
HpyCH4IV ACGT 5 cut(s) 43, 204, 244, 720, 909
HpyCH4V TGCA 2 cut(s) 119, 1037
HpyF10VI GCNNNNNNNGC 5 cut(s) 673, 782, 976, 1137, 1216
HpyF3I CTNAG 5 cut(s) 302, 548, 646, 915, 1195
HpySE526I ACGT 5 cut(s) 43, 204, 244, 720, 909
Hsp92II CATG 6 cut(s) 61, 501, 853, 944, 963, 1012
KspI CCGCGG 1 cut(s) 933
LmnI GCTCC 2 cut(s) 243, 1216
Lsp1109I GCAGC 1 cut(s) 991
LweI GCATC 1 cut(s) 991
MaeI CTAG 2 cut(s) 1179, 1191
MaeII ACGT 5 cut(s) 43, 204, 244, 720, 909
MaeIII GTNAC 5 cut(s) 454, 571, 884, 905, 1234
MbiI CCGCTC 1 cut(s) 1029
MflI RGATCY 4 cut(s) 266, 277, 346, 1031
MhlI GDGCHC 2 cut(s) 636, 1160
MluCI AATT 5 cut(s) 24, 684, 829, 862, 1247
MlyI GAGTC 2 cut(s) 568, 690
MmeI TCCRAC 1 cut(s) 1025
MnlI CCTC 6 cut(s) 231, 578, 969, 996, 1200, 1234
MseI TTAA 6 cut(s) 140, 165, 513, 621, 680, 707
MslI CAYNNNNRTG 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 932
MspI CCGG 4 cut(s) 359, 465, 524, 613
MspR9I CCNGG 3 cut(s) 524, 693, 1128
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 2 cut(s) 693, 1128
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 5 cut(s) 673, 782, 976, 1137, 1216
NciI CCSGG 1 cut(s) 524
NdeI CATATG 1 cut(s) 902
NlaIII CATG 6 cut(s) 61, 501, 853, 944, 963, 1012
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 2 cut(s) 454, 571
NspI RCATGY 1 cut(s) 501
NspV TTCGAA 1 cut(s) 200
OliI CACNNNNGTG 1 cut(s) 498
PceI AGGCCT 1 cut(s) 1189
PctI GAATGC 1 cut(s) 797
PfeI GAWTC 5 cut(s) 197, 353, 505, 589, 1258
PfoI TCCNGGA 1 cut(s) 691
PinAI ACCGGT 1 cut(s) 358
PkrI GCNGC 1 cut(s) 981
PleI GAGTC 2 cut(s) 568, 690
PpsI GAGTC 2 cut(s) 568, 690
Ppu21I YACGTR 1 cut(s) 44
PshBI ATTAAT 2 cut(s) 165, 680
Psp1406I AACGTT 2 cut(s) 204, 720
Psp6I CCWGG 2 cut(s) 691, 1126
PspGI CCWGG 2 cut(s) 691, 1126
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 2 cut(s) 467, 610
PsrI GAACNNNNNNTAC 2 cut(s) 1083, 1115
PstNI CAGNNNCTG 1 cut(s) 1202
PsuI RGATCY 4 cut(s) 266, 277, 346, 1031
RsaI GTAC 3 cut(s) 42, 314, 854
RsaNI GTAC 3 cut(s) 41, 313, 853
RseI CAYNNNNRTG 1 cut(s) 498
SacII CCGCGG 1 cut(s) 933
SaqAI TTAA 6 cut(s) 140, 165, 513, 621, 680, 707
SatI GCNGC 1 cut(s) 980
Sau96I GGNCC 2 cut(s) 467, 610
SchI GAGTC 2 cut(s) 568, 690
ScrFI CCNGG 3 cut(s) 524, 693, 1128
SduI GDGCHC 2 cut(s) 636, 1160
SfaNI GCATC 1 cut(s) 991
Sfr303I CCGCGG 1 cut(s) 933
SfuI TTCGAA 1 cut(s) 200
SgrBI CCGCGG 1 cut(s) 933
SinI GGWCC 2 cut(s) 467, 610
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 3 cut(s) 12, 576, 1211
SmoI CTYRAG 3 cut(s) 12, 576, 1211
SnaBI TACGTA 1 cut(s) 44
Sse9I AATT 5 cut(s) 24, 684, 829, 862, 1247
SseBI AGGCCT 1 cut(s) 1189
SsiI CCGC 7 cut(s) 667, 774, 930, 932, 1029, 1131, 1140
SspMI CTAG 2 cut(s) 1179, 1191
StuI AGGCCT 1 cut(s) 1189
StyD4I CCNGG 3 cut(s) 522, 691, 1126
TaaI ACNGT 4 cut(s) 821, 898, 1069, 1118
TaiI ACGT 5 cut(s) 46, 207, 247, 723, 912
TaqI TCGA 7 cut(s) 154, 200, 602, 630, 742, 1025, 1167
TasI AATT 5 cut(s) 24, 684, 829, 862, 1247
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 5 cut(s) 197, 353, 505, 589, 1258
Tru1I TTAA 6 cut(s) 140, 165, 513, 621, 680, 707
Tru9I TTAA 6 cut(s) 140, 165, 513, 621, 680, 707
TseFI GTSAC 2 cut(s) 454, 571
TseI GCWGC 1 cut(s) 979
Tsp45I GTSAC 2 cut(s) 454, 571
TspDTI ATGAA 2 cut(s) 69, 581
TspGWI ACGGA 1 cut(s) 1117
VpaK11BI GGWCC 2 cut(s) 467, 610
VspI ATTAAT 2 cut(s) 165, 680
XagI CCTNNNNNAGG 1 cut(s) 1194
XapI RAATTY 1 cut(s) 24
XceI RCATGY 1 cut(s) 501
XspI CTAG 2 cut(s) 1179, 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.