AT3G15350

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007023
Physical Location & Seq
Forward (+)
5166175 .. 5169229
3055 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G15350.1

Sequence Viewer

Length: 1275 bp
ATGGGGTATGTAAACGTGGAGAAGAGATGGGTTTTTCCTCTTGTCATCACATCTCTCGTCTGCGTTTTCCTTCTTGCAACTTCTTTCAACATGGGACTAGTCTCTTCCCTCCGAACAATCAACGGGATTTTCTCCATCATACCTTCCCGTCTCGTCAAGAACCAAACGAGACTTGATTTTGCAGAGTCTAAAGTTGCTAGACAAACCCGTGTTTTGCCTCATGAAGACAAGCTTCCCCGTTTTGCATACCTTGTTTCCGGGTCTAAAGGGGATGTAGAAAAGCTTTGGAGAACACTTAGAGCAGTGTACCATCCAAGAAACCAGTATGTTGTTCACTTGGATCTTGAATCACCTGTGAATGAGAGATTAGAGCTGGCCTCTCGGATTAATAACGATCCTATGTACTCTAAAACCGGGAATGTATATATGATAACCAAGGCTAACCTTGTGACATATAAAGGACCAACGATGGTGGCAAATACTCTTCACGCTTGTGCTGTTCTTCTCAAGAGAAATGCGAACTGGGATTGGTTTATTAACCTTAGTGCTTCAGATTATCCTCTGGTGACCCAAGATGATCTGCTTCATACGTTTTCAACATTGGACCGGAACCTCAACTTTATCGAACACACAAGTCAACTGGGATGGAAAGAGGAGAAGCGAGCACAGCCATTAATGATTGATCCTGGACTCTACTTGTTGAACAAATCTGATATTTACTGGGTCACACCTCGCCGAAGTTTGCCAACTGCCTTTAAGTTATTCACTGGATCAGCTTGGATGGCTCTATCACGCCCGTTTGTAGAGTATTGCATATGGGGATGGGACAATTTACCACGAACTCTCCTAATGTATTACACCAACTTCGTTTCATCGCCCGAAGGTTACTTCCAGACCGTGATTTGCAACGTGCCTGAGTTTGCAAAAACTGCAGTAAACCATGACCTACACTACATTTCCTGGGACAATCCGCCGCAGCAGCACCCACACGTGTTGTCACTCAACGACACAATGCCGATGATATGGAGCGGTGCTGCGTTTGCTAGGAAATTCAGAAGAGATGATGAAGTACTTAATAAGATCGATAAGGAATTACTTAAACGGAGGAATGATAAAGATAGTTTTACTCCTGGTGGATGGTGCAGTGGGAAACCAAAATGCTCCAGGGTCGGTAATGTGGCTAAGATTGTTCCTAGCTTTGGAGCTCAGAGGCTTCAAGGACTTGTGACCAGGTTAGTCAACGAAGCTAATACAGGAGTTAGTCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

424

Amino Acids

48.54

Weight (kDa)

9.41

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 81 - 338 2.5e-81 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1029
AciI CCGC 3 cut(s) 971, 974, 1029
AclWI GGATC 4 cut(s) 348, 389, 677, 778
AcsI RAATTY 1 cut(s) 1049
AcuI CTGAAG 1 cut(s) 534
AcvI CACGTG 1 cut(s) 991
AfaI GTAC 3 cut(s) 308, 404, 1071
AfiI CCNNNNNNNGG 1 cut(s) 1199
AflIII ACRYGT 2 cut(s) 988, 990
AgsI TTSAA 5 cut(s) 88, 347, 597, 703, 1217
AhlI ACTAGT 1 cut(s) 97
AjnI CCWGG 5 cut(s) 685, 959, 1129, 1163, 1229
AleI CACNNNNGTG 1 cut(s) 492
AluBI AGCT 7 cut(s) 232, 283, 373, 776, 1197, 1205, 1247
AluI AGCT 7 cut(s) 232, 283, 373, 776, 1197, 1205, 1247
Alw21I GWGCWC 2 cut(s) 667, 1207
Alw26I GTCTC 3 cut(s) 106, 155, 163
AlwI GGATC 4 cut(s) 348, 389, 677, 778
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 3 cut(s) 976, 979, 1034
ApoI RAATTY 1 cut(s) 1049
AseI ATTAAT 2 cut(s) 387, 674
AspS9I GGNCC 2 cut(s) 461, 604
AsuC2I CCSGG 2 cut(s) 259, 415
AsuHPI GGTGA 2 cut(s) 342, 577
AvaII GGWCC 2 cut(s) 461, 604
BanII GRGCYC 1 cut(s) 1207
BbrPI CACGTG 1 cut(s) 991
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 667, 1207
BbvI GCAGC 3 cut(s) 988, 991, 1021
BccI CCATC 8 cut(s) 21, 143, 318, 463, 639, 775, 816, 1131
BciT130I CCWGG 5 cut(s) 687, 961, 1131, 1165, 1231
BcnI CCSGG 2 cut(s) 259, 415
BcoDI GTCTC 3 cut(s) 106, 155, 163
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 4 cut(s) 98, 198, 1044, 1194
BfmI CTRYAG 1 cut(s) 930
BisI GCNGC 4 cut(s) 974, 977, 980, 1035
BlsI GCNGC 4 cut(s) 975, 978, 981, 1036
BmcAI AGTACT 1 cut(s) 1071
Bme1390I CCNGG 7 cut(s) 259, 415, 687, 961, 1131, 1165, 1231
Bme18I GGWCC 2 cut(s) 461, 604
BmgT120I GGNCC 2 cut(s) 461, 604
BmiI GGNNCC 1 cut(s) 611
BmrFI CCNGG 7 cut(s) 259, 415, 687, 961, 1131, 1165, 1231
BmrI ACTGGG 3 cut(s) 532, 650, 730
BmuI ACTGGG 3 cut(s) 532, 650, 730
BpiI GAAGAC 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpmI CTGGAG 1 cut(s) 1147
BpuEI CTTGAG 1 cut(s) 491
BpuMI CCSGG 2 cut(s) 259, 415
Bsa29I ATCGAT 1 cut(s) 1083
BsaAI YACGTR 1 cut(s) 991
BsaJI CCNNGG 3 cut(s) 435, 960, 1164
BsaWI WCCGGW 1 cut(s) 606
BsaXI ACNNNNNCTCC 2 cut(s) 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 1199
Bse1I ACTGG 5 cut(s) 322, 527, 645, 725, 772
BseBI CCWGG 5 cut(s) 687, 961, 1131, 1165, 1231
BseCI ATCGAT 1 cut(s) 1083
BseDI CCNNGG 3 cut(s) 435, 960, 1164
BseGI GGATG 6 cut(s) 277, 310, 650, 786, 827, 1142
BseLI CCNNNNNNNGG 1 cut(s) 1199
BseMII CTCAG 2 cut(s) 906, 1220
BseNI ACTGG 5 cut(s) 322, 527, 645, 725, 772
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 3 cut(s) 988, 991, 1021
BsgI GTGCAG 1 cut(s) 1162
BshFI GGCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 1083
BsiHKAI GWGCWC 2 cut(s) 667, 1207
BsiSI CCGG 3 cut(s) 258, 414, 607
BslFI GGGAC 3 cut(s) 108, 839, 977
BslI CCNNNNNNNGG 1 cut(s) 1199
BsmAI GTCTC 3 cut(s) 106, 155, 163
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 3 cut(s) 108, 839, 977
BsnI GGCC 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 667, 1207
Bsp143I GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
BspACI CCGC 3 cut(s) 971, 974, 1029
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 2 cut(s) 907, 1219
BspDI ATCGAT 1 cut(s) 1083
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 611
BspMAI CTGCAG 1 cut(s) 934
BspPI GGATC 4 cut(s) 348, 389, 677, 778
BsrBI CCGCTC 1 cut(s) 1029
BsrI ACTGG 5 cut(s) 322, 527, 645, 725, 772
BssECI CCNNGG 3 cut(s) 435, 960, 1164
BssMI GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 5 cut(s) 687, 961, 1131, 1165, 1231
Bst4CI ACNGT 1 cut(s) 898
Bst6I CTCTTC 4 cut(s) 17, 109, 489, 1051
BstAPI GCANNNNNTGC 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 991
BstC8I GCNNGC 2 cut(s) 375, 663
BstDEI CTNAG 5 cut(s) 296, 542, 915, 1182, 1206
BstEII GGTNACC 1 cut(s) 565
BstF5I GGATG 6 cut(s) 277, 310, 650, 786, 827, 1142
BstKTI GATC 6 cut(s) 343, 397, 580, 685, 773, 1083
BstMAI GTCTC 3 cut(s) 106, 155, 163
BstMBI GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
BstMWI GCNNNNNNNGC 5 cut(s) 667, 782, 929, 979, 1040
BstNI CCWGG 5 cut(s) 687, 961, 1131, 1165, 1231
BstPI GGTNACC 1 cut(s) 565
BstSCI CCNGG 7 cut(s) 257, 413, 685, 959, 1129, 1163, 1229
BstSFI CTRYAG 1 cut(s) 930
BstV1I GCAGC 3 cut(s) 988, 991, 1021
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 1083
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 1083
BtgZI GCGATG 1 cut(s) 858
BtsCI GGATG 6 cut(s) 277, 310, 650, 786, 827, 1142
BtsI GCAGTG 2 cut(s) 309, 1150
BtsIMutI CAGTG 4 cut(s) 309, 765, 1150, 1271
Cac8I GCNNGC 2 cut(s) 375, 663
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 461, 604
ClaI ATCGAT 1 cut(s) 1083
CsiI ACCWGGT 1 cut(s) 1229
Csp6I GTAC 3 cut(s) 307, 403, 1070
CspCI CAANNNNNGTGG 4 cut(s) 453, 488, 975, 1010
CviAII CATG 3 cut(s) 91, 221, 941
CviQI GTAC 3 cut(s) 307, 403, 1070
DdeI CTNAG 5 cut(s) 296, 542, 915, 1182, 1206
DpnI GATC 6 cut(s) 342, 396, 579, 684, 772, 1082
DpnII GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
Eam1104I CTCTTC 4 cut(s) 17, 109, 489, 1051
EarI CTCTTC 4 cut(s) 17, 109, 489, 1051
EciI GGCGGA 1 cut(s) 960
Ecl136II GAGCTC 1 cut(s) 1205
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 1207
Eco47I GGWCC 2 cut(s) 461, 604
Eco53kI GAGCTC 1 cut(s) 1205
Eco57I CTGAAG 1 cut(s) 534
Eco72I CACGTG 1 cut(s) 991
Eco91I GGTNACC 1 cut(s) 565
EcoICRI GAGCTC 1 cut(s) 1205
EcoO65I GGTNACC 1 cut(s) 565
EcoRII CCWGG 5 cut(s) 685, 959, 1129, 1163, 1229
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 1207
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 3 cut(s) 94, 224, 944
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 3 cut(s) 108, 839, 977
FatI CATG 3 cut(s) 90, 220, 940
FauNDI CATATG 1 cut(s) 815
Fnu4HI GCNGC 4 cut(s) 974, 977, 980, 1035
FokI GGATG 6 cut(s) 284, 297, 657, 793, 834, 1149
FriOI GRGCYC 1 cut(s) 1207
Fsp4HI GCNGC 4 cut(s) 974, 977, 980, 1035
FspBI CTAG 4 cut(s) 98, 198, 1044, 1194
GluI GCNGC 4 cut(s) 974, 977, 980, 1035
GsuI CTGGAG 1 cut(s) 1147
HaeIII GGCC 1 cut(s) 377
HapII CCGG 3 cut(s) 258, 414, 607
Hin1II CATG 3 cut(s) 94, 224, 944
HincII GTYRAC 2 cut(s) 638, 1240
HindII GTYRAC 2 cut(s) 638, 1240
HindIII AAGCTT 2 cut(s) 230, 281
HinfI GANTC 3 cut(s) 185, 347, 690
HpaII CCGG 3 cut(s) 258, 414, 607
HphI GGTGA 2 cut(s) 342, 577
Hpy166II GTNNAC 6 cut(s) 13, 307, 334, 638, 937, 1240
Hpy188I TCNGA 6 cut(s) 113, 384, 553, 712, 1055, 1209
Hpy188III TCNNGA 5 cut(s) 157, 221, 344, 508, 892
Hpy8I GTNNAC 6 cut(s) 13, 307, 334, 638, 937, 1240
HpyAV CCTTC 3 cut(s) 80, 153, 875
HpyCH4III ACNGT 1 cut(s) 898
HpyCH4IV ACGT 4 cut(s) 15, 590, 909, 990
HpyCH4V TGCA 8 cut(s) 77, 182, 245, 813, 906, 923, 932, 1143
HpyF10VI GCNNNNNNNGC 5 cut(s) 667, 782, 929, 979, 1040
HpyF3I CTNAG 5 cut(s) 296, 542, 915, 1182, 1206
HpySE526I ACGT 4 cut(s) 15, 590, 909, 990
Hsp92II CATG 3 cut(s) 94, 224, 944
Kzo9I GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
LmnI GCTCC 3 cut(s) 1026, 1166, 1202
Lsp1109I GCAGC 3 cut(s) 988, 991, 1021
MabI ACCWGGT 1 cut(s) 1229
MaeI CTAG 4 cut(s) 98, 198, 1044, 1194
MaeII ACGT 4 cut(s) 15, 590, 909, 990
MaeIII GTNAC 6 cut(s) 448, 565, 724, 884, 996, 1225
MalI GATC 6 cut(s) 342, 396, 579, 684, 772, 1082
MbiI CCGCTC 1 cut(s) 1029
MboI GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
MboII GAAGA 6 cut(s) 34, 96, 236, 476, 494, 1068
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 667, 1207
MluCI AATT 3 cut(s) 829, 1049, 1091
MlyI GAGTC 2 cut(s) 194, 684
MseI TTAA 6 cut(s) 387, 537, 674, 756, 1074, 1098
MslI CAYNNNNRTG 1 cut(s) 492
MspI CCGG 3 cut(s) 258, 414, 607
MspR9I CCNGG 7 cut(s) 259, 415, 687, 961, 1131, 1165, 1231
MvaI CCWGG 5 cut(s) 687, 961, 1131, 1165, 1231
MwoI GCNNNNNNNGC 5 cut(s) 667, 782, 929, 979, 1040
NciI CCSGG 2 cut(s) 259, 415
NdeI CATATG 1 cut(s) 815
NdeII GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
NlaIII CATG 3 cut(s) 94, 224, 944
NlaIV GGNNCC 1 cut(s) 611
NmuCI GTSAC 5 cut(s) 448, 565, 724, 996, 1225
OliI CACNNNNGTG 1 cut(s) 492
PagI TCATGA 1 cut(s) 220
PfeI GAWTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 685
PkrI GCNGC 4 cut(s) 975, 978, 981, 1036
PleI GAGTC 2 cut(s) 193, 684
PmaCI CACGTG 1 cut(s) 991
PmlI CACGTG 1 cut(s) 991
PpsI GAGTC 2 cut(s) 193, 684
Ppu21I YACGTR 1 cut(s) 991
PshBI ATTAAT 2 cut(s) 387, 674
Psp124BI GAGCTC 1 cut(s) 1207
Psp6I CCWGG 5 cut(s) 685, 959, 1129, 1163, 1229
PspCI CACGTG 1 cut(s) 991
PspEI GGTNACC 1 cut(s) 565
PspGI CCWGG 5 cut(s) 685, 959, 1129, 1163, 1229
PspN4I GGNNCC 1 cut(s) 611
PspPI GGNCC 2 cut(s) 461, 604
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 3 cut(s) 308, 404, 1071
RsaNI GTAC 3 cut(s) 307, 403, 1070
RseI CAYNNNNRTG 1 cut(s) 492
SacI GAGCTC 1 cut(s) 1207
SaqAI TTAA 6 cut(s) 387, 537, 674, 756, 1074, 1098
SatI GCNGC 4 cut(s) 974, 977, 980, 1035
Sau3AI GATC 6 cut(s) 340, 394, 577, 682, 770, 1080
Sau96I GGNCC 2 cut(s) 461, 604
ScaI AGTACT 1 cut(s) 1071
SchI GAGTC 2 cut(s) 194, 684
ScrFI CCNGG 7 cut(s) 259, 415, 687, 961, 1131, 1165, 1231
SduI GDGCHC 2 cut(s) 667, 1207
SexAI ACCWGGT 1 cut(s) 1229
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 2 cut(s) 461, 604
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SpeI ACTAGT 1 cut(s) 97
Sse9I AATT 3 cut(s) 829, 1049, 1091
SsiI CCGC 3 cut(s) 971, 974, 1029
SspMI CTAG 4 cut(s) 98, 198, 1044, 1194
SstI GAGCTC 1 cut(s) 1207
StyD4I CCNGG 7 cut(s) 257, 413, 685, 959, 1129, 1163, 1229
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 898
TaiI ACGT 4 cut(s) 18, 593, 912, 993
TaqI TCGA 2 cut(s) 624, 1083
TasI AATT 3 cut(s) 829, 1049, 1091
TatI WGTACW 2 cut(s) 402, 1069
TauI GCSGC 1 cut(s) 976
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 6 cut(s) 387, 537, 674, 756, 1074, 1098
Tru9I TTAA 6 cut(s) 387, 537, 674, 756, 1074, 1098
TscAI CASTG 4 cut(s) 309, 772, 1150, 1271
TseFI GTSAC 5 cut(s) 448, 565, 724, 996, 1225
TseI GCWGC 3 cut(s) 976, 979, 1034
Tsp45I GTSAC 5 cut(s) 448, 565, 724, 996, 1225
TspDTI ATGAA 4 cut(s) 237, 575, 861, 1080
TspGWI ACGGA 1 cut(s) 1117
TspRI CASTG 4 cut(s) 309, 772, 1150, 1271
VpaK11BI GGWCC 2 cut(s) 461, 604
VspI ATTAAT 2 cut(s) 387, 674
XapI RAATTY 1 cut(s) 1049
XspI CTAG 4 cut(s) 98, 198, 1044, 1194
ZrmI AGTACT 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.