MD03G1228200.v1.1

Xylosyltransferase 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007023
Physical Location & Seq
Reverse (-)
31361496 .. 31365079
3584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1228200.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGGGGTCCGCAATGGAGAAGAAATGGCTATTCCCTCTTCTCATAAGTTCTGTCATATGCATATTCCTTCTAGCCACCTCCATGAACTTGGGTCTCGTATCTTCACGACAAGCAATCAATTCGATCTTTTCCTTTTTACCATCTAGGGTGGTAACAAACCAATCTAGTCCAGCTTTTGCTGAGACTATAGTTTCACAAGGTCCACCCCCTCCTCCAGTGCCATCGATTCCCCGCTTTGCTTATCTAATTTCCGGGTCAAAGGGGGATTTGGAAAAGCTTTGGAGAACTCTCAAAGCGCTTTACCATCCATTGAATCAATATGTTCTTCATTTAGACCTTGAGTCGCCGTTAGCAGAAAGATTGGAGCTTGCTTCGCGAGTGGACAACGAGACTCTCTTTAATACAGTTGGGAATGTTTTCATGATCAAGAAAGCTAATATGGTAACTTACAGAGGACCAACAATGGTTGCTAATACTCTTCATGCCTGCGCAATTCTTCTCAGGAGGAGTAAGGATTGGGATTGGTTTATCAATCTCAGCGCCTCAGATTATCCGCTTGTTACTCAAGATGATCTTATTAACACTTTTTCAACTTTAAACCGAAATCTGAATTTCATTGAGCACACAAGCCAACTTGGCTGGAAGGAGGATAAACGAGCAATGCCTTTGATTTTAGACCCTGGTCTCTACTCATCGAAAAAACAAGATGTATTTTGGGTTACACCGCGGCGAACATTGCCCACTTCGTTTAAATTGTTTACTGGTTCGGCATGGATGGTCTTATCACGCTGGTTCGTTGAGTACTGCGTTTGGGGTTGGGACAATCTTCCGAGGACCCTTCTCATGTACTACACAAACTTTGTTTCTTCGCCAGAAGGCTACTTTCACACTGTTTTATGCAATGAGCCGGAGTTTGCTAAGACTGCCGTCAACCATGATTTGCACTACATTTCTTGGGACATTCCTCCCAAGCAGCATCCTCATACCCTCAACATTAATGACACAAACAAGATGATTGCAAGTGGTGCTGCCTTTGCTCGAAAATTCAGGCACGACGACCCCGTCCTGGACAGGATCGACAAGGAATTACTTCACCGAAGAAAAGAGAGCTTCACTCCCGGTGGTTGGTGTGCTGGCAAACCGAAATGCTCCAGGGTTGGTAACCCGAACAAGATCAAACCAAGTCCAGGCGCCGACAGGCTTCGCCACCTCGTGGGTAGGCTTACCTTGACAGCCAAGTTTGGTCAAAACCAGTGTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

421

Amino Acids

47.78

Weight (kDa)

9.44

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 79 - 338 1.7e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1061
Acc16I TGCGCA 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 1190
AccB7I CCANNNNNTGG 1 cut(s) 1213
AccII CGCG 2 cut(s) 376, 727
AciI CCGC 5 cut(s) 9, 232, 554, 725, 727
AclWI GGATC 1 cut(s) 1082
AcsI RAATTY 2 cut(s) 610, 1043
AcyI GRCGYC 1 cut(s) 1191
AdeI CACNNNGTG 1 cut(s) 1213
AfaI GTAC 2 cut(s) 803, 848
AfeI AGCGCT 1 cut(s) 297
AfiI CCNNNNNNNGG 4 cut(s) 1066, 1125, 1187, 1213
AgsI TTSAA 2 cut(s) 313, 591
AhdI GACNNNNNGTC 1 cut(s) 340
AjnI CCWGG 4 cut(s) 679, 1065, 1151, 1186
AluBI AGCT 5 cut(s) 173, 277, 367, 434, 1110
AluI AGCT 5 cut(s) 173, 277, 367, 434, 1110
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 4 cut(s) 98, 176, 383, 689
AlwI GGATC 1 cut(s) 1082
Aor51HI AGCGCT 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 973, 1028
ApoI RAATTY 2 cut(s) 610, 1043
AseI ATTAAT 1 cut(s) 996
Asp700I GAANNNNTTC 2 cut(s) 416, 1089
AspLEI GCGC 4 cut(s) 298, 491, 542, 1193
AspS9I GGNCC 4 cut(s) 6, 200, 455, 834
AsuC2I CCSGG 2 cut(s) 253, 1119
AsuHPI GGTGA 1 cut(s) 1085
AvaII GGWCC 4 cut(s) 6, 200, 455, 834
BanI GGYRCC 1 cut(s) 1190
BauI CACGAG 1 cut(s) 1211
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 2 cut(s) 985, 1015
BccI CCATC 4 cut(s) 148, 229, 312, 769
BceAI ACGGC 2 cut(s) 331, 911
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 4 cut(s) 681, 1067, 1153, 1188
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 2 cut(s) 253, 1119
BcoDI GTCTC 4 cut(s) 98, 176, 383, 689
BfaI CTAG 3 cut(s) 71, 144, 165
BfmI CTRYAG 1 cut(s) 186
BfoI RGCGCY 3 cut(s) 299, 543, 1194
BglI GCCNNNNNGGC 1 cut(s) 636
BisI GCNGC 3 cut(s) 728, 974, 1029
BlsI GCNGC 3 cut(s) 729, 975, 1030
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 6 cut(s) 253, 681, 1067, 1119, 1153, 1188
Bme18I GGWCC 4 cut(s) 6, 200, 455, 834
BmeRI GACNNNNNGTC 1 cut(s) 340
BmgT120I GGNCC 4 cut(s) 6, 200, 455, 834
BmiI GGNNCC 3 cut(s) 7, 836, 1192
BmrFI CCNGG 6 cut(s) 253, 681, 1067, 1119, 1153, 1188
BmsI GCATC 1 cut(s) 985
BoxI GACNNNNGTC 2 cut(s) 681, 926
BplI GAGNNNNNCTC 2 cut(s) 1099, 1131
BpmI CTGGAG 2 cut(s) 198, 1135
BpuEI CTTGAG 2 cut(s) 359, 549
BpuMI CCSGG 2 cut(s) 253, 1119
Bsa29I ATCGAT 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 1191
BsaI GGTCTC 2 cut(s) 98, 689
BsaJI CCNNGG 4 cut(s) 679, 725, 830, 1152
Bsc4I CCNNNNNNNGG 4 cut(s) 1066, 1125, 1187, 1213
Bse1I ACTGG 3 cut(s) 215, 766, 1252
Bse3DI GCAATG 4 cut(s) 18, 666, 734, 907
BseBI CCWGG 4 cut(s) 681, 1067, 1153, 1188
BseCI ATCGAT 1 cut(s) 224
BseDI CCNNGG 4 cut(s) 679, 725, 830, 1152
BseGI GGATG 3 cut(s) 304, 780, 976
BseLI CCNNNNNNNGG 4 cut(s) 1066, 1125, 1187, 1213
BseMI GCAATG 4 cut(s) 18, 666, 734, 907
BseMII CTCAG 4 cut(s) 171, 514, 550, 558
BseNI ACTGG 3 cut(s) 215, 766, 1252
BseRI GAGGAG 2 cut(s) 201, 520
BseXI GCAGC 2 cut(s) 985, 1015
Bsh1236I CGCG 2 cut(s) 376, 727
BshNI GGYRCC 1 cut(s) 1190
BshVI ATCGAT 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 624
BsiSI CCGG 3 cut(s) 252, 908, 1119
BslFI GGGAC 2 cut(s) 833, 971
BslI CCNNNNNNNGG 4 cut(s) 1066, 1125, 1187, 1213
BsmAI GTCTC 4 cut(s) 98, 176, 383, 689
BsmFI GGGAC 2 cut(s) 833, 971
Bso31I GGTCTC 2 cut(s) 98, 689
Bsp1286I GDGCHC 1 cut(s) 624
Bsp143I GATC 5 cut(s) 123, 423, 571, 1074, 1173
Bsp68I TCGCGA 1 cut(s) 376
BspACI CCGC 5 cut(s) 9, 232, 554, 725, 727
BspCNI CTCAG 4 cut(s) 172, 513, 549, 557
BspDI ATCGAT 1 cut(s) 224
BspFNI CGCG 2 cut(s) 376, 727
BspHI TCATGA 1 cut(s) 420
BspLI GGNNCC 3 cut(s) 7, 836, 1192
BspPI GGATC 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 1190
BspTNI GGTCTC 2 cut(s) 98, 689
BsrDI GCAATG 4 cut(s) 18, 666, 734, 907
BsrI ACTGG 3 cut(s) 215, 766, 1252
BssECI CCNNGG 4 cut(s) 679, 725, 830, 1152
BssMI GATC 5 cut(s) 123, 423, 571, 1074, 1173
BssNI GRCGYC 1 cut(s) 1191
BssSI CACGAG 1 cut(s) 1211
Bst2BI CACGAG 1 cut(s) 1211
Bst2UI CCWGG 4 cut(s) 681, 1067, 1153, 1188
Bst4CI ACNGT 2 cut(s) 406, 892
Bst6I CTCTTC 2 cut(s) 42, 483
BstACI GRCGYC 1 cut(s) 1191
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstC8I GCNNGC 3 cut(s) 369, 487, 1135
BstDEI CTNAG 5 cut(s) 180, 500, 536, 544, 918
BstDSI CCRYGG 1 cut(s) 725
BstEII GGTNACC 1 cut(s) 1160
BstF5I GGATG 3 cut(s) 304, 780, 976
BstFNI CGCG 2 cut(s) 376, 727
BstH2I RGCGCY 3 cut(s) 299, 543, 1194
BstHHI GCGC 4 cut(s) 298, 491, 542, 1193
BstKTI GATC 5 cut(s) 126, 426, 574, 1077, 1176
BstMAI GTCTC 4 cut(s) 98, 176, 383, 689
BstMBI GATC 5 cut(s) 123, 423, 571, 1074, 1173
BstMWI GCNNNNNNNGC 6 cut(s) 373, 636, 736, 923, 1025, 1034
BstNI CCWGG 4 cut(s) 681, 1067, 1153, 1188
BstPAI GACNNNNGTC 2 cut(s) 681, 926
BstPI GGTNACC 1 cut(s) 1160
BstSCI CCNGG 6 cut(s) 251, 679, 1065, 1117, 1151, 1186
BstSFI CTRYAG 1 cut(s) 186
BstUI CGCG 2 cut(s) 376, 727
BstV1I GCAGC 2 cut(s) 985, 1015
BstXI CCANNNNNNTGG 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 224
BsuTUI ATCGAT 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 725
BtsCI GGATG 3 cut(s) 304, 780, 976
BtsIMutI CAGTG 3 cut(s) 222, 888, 1259
BtuMI TCGCGA 1 cut(s) 376
Cac8I GCNNGC 3 cut(s) 369, 487, 1135
CciI TCATGA 1 cut(s) 420
CfoI GCGC 4 cut(s) 298, 491, 542, 1193
Cfr13I GGNCC 4 cut(s) 6, 200, 455, 834
Cfr42I CCGCGG 1 cut(s) 728
ClaI ATCGAT 1 cut(s) 224
Csp6I GTAC 2 cut(s) 802, 847
CviAII CATG 6 cut(s) 82, 421, 482, 771, 844, 935
CviQI GTAC 2 cut(s) 802, 847
DdeI CTNAG 5 cut(s) 180, 500, 536, 544, 918
DinI GGCGCC 1 cut(s) 1192
DpnI GATC 5 cut(s) 125, 425, 573, 1076, 1175
DpnII GATC 5 cut(s) 123, 423, 571, 1074, 1173
DraI TTTAAA 2 cut(s) 597, 751
DraIII CACNNNGTG 1 cut(s) 1213
DrdI GACNNNNNNGTC 1 cut(s) 1061
DriI GACNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 1061
Eam1104I CTCTTC 2 cut(s) 42, 483
Eam1105I GACNNNNNGTC 1 cut(s) 340
EarI CTCTTC 2 cut(s) 42, 483
Eco31I GGTCTC 2 cut(s) 98, 689
Eco47I GGWCC 4 cut(s) 6, 200, 455, 834
Eco47III AGCGCT 1 cut(s) 297
Eco91I GGTNACC 1 cut(s) 1160
EcoO109I RGGNCCY 1 cut(s) 834
EcoO65I GGTNACC 1 cut(s) 1160
EcoRII CCWGG 4 cut(s) 679, 1065, 1151, 1186
EcoT22I ATGCAT 1 cut(s) 62
EgeI GGCGCC 1 cut(s) 1192
EheI GGCGCC 1 cut(s) 1192
FaeI CATG 6 cut(s) 85, 424, 485, 774, 847, 938
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 2 cut(s) 833, 971
FatI CATG 6 cut(s) 81, 420, 481, 770, 843, 934
FauI CCCGC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 56
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 728, 974, 1029
FokI GGATG 3 cut(s) 291, 787, 963
Fsp4HI GCNGC 3 cut(s) 728, 974, 1029
FspBI CTAG 3 cut(s) 71, 144, 165
FspI TGCGCA 1 cut(s) 490
GlaI GCGC 4 cut(s) 297, 490, 541, 1192
GluI GCNGC 3 cut(s) 728, 974, 1029
GsuI CTGGAG 2 cut(s) 198, 1135
HaeII RGCGCY 3 cut(s) 299, 543, 1194
HapII CCGG 3 cut(s) 252, 908, 1119
HhaI GCGC 4 cut(s) 298, 491, 542, 1193
Hin1I GRCGYC 1 cut(s) 1191
Hin1II CATG 6 cut(s) 85, 424, 485, 774, 847, 938
Hin6I GCGC 4 cut(s) 296, 489, 540, 1191
HinP1I GCGC 4 cut(s) 296, 489, 540, 1191
HincII GTYRAC 1 cut(s) 931
HindII GTYRAC 1 cut(s) 931
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 4 cut(s) 226, 313, 341, 391
HpaII CCGG 3 cut(s) 252, 908, 1119
HphI GGTGA 1 cut(s) 1085
Hpy166II GTNNAC 4 cut(s) 203, 382, 759, 931
Hpy188I TCNGA 3 cut(s) 547, 609, 831
Hpy188III TCNNGA 6 cut(s) 105, 375, 421, 427, 502, 566
Hpy8I GTNNAC 4 cut(s) 203, 382, 759, 931
Hpy99I CGWCG 1 cut(s) 1058
HpyAV CCTTC 4 cut(s) 77, 637, 848, 869
HpyCH4III ACNGT 2 cut(s) 406, 892
HpyCH4V TGCA 4 cut(s) 60, 900, 943, 1019
HpyF10VI GCNNNNNNNGC 6 cut(s) 373, 636, 736, 923, 1025, 1034
HpyF3I CTNAG 5 cut(s) 180, 500, 536, 544, 918
Hsp92I GRCGYC 1 cut(s) 1191
Hsp92II CATG 6 cut(s) 85, 424, 485, 774, 847, 938
HspAI GCGC 4 cut(s) 296, 489, 540, 1191
KasI GGCGCC 1 cut(s) 1190
Ksp22I TGATCA 1 cut(s) 423
KspI CCGCGG 1 cut(s) 728
Kzo9I GATC 5 cut(s) 123, 423, 571, 1074, 1173
LmnI GCTCC 2 cut(s) 364, 1154
Lsp1109I GCAGC 2 cut(s) 985, 1015
LweI GCATC 1 cut(s) 985
MaeI CTAG 3 cut(s) 71, 144, 165
MaeIII GTNAC 5 cut(s) 151, 442, 559, 718, 1160
MalI GATC 5 cut(s) 125, 425, 573, 1076, 1175
MboI GATC 5 cut(s) 123, 423, 571, 1074, 1173
MboII GAAGA 9 cut(s) 29, 31, 93, 317, 470, 488, 818, 858, 1110
MhlI GDGCHC 1 cut(s) 624
MluCI AATT 7 cut(s) 118, 246, 492, 610, 752, 1043, 1085
Mly113I GGCGCC 1 cut(s) 1191
MlyI GAGTC 2 cut(s) 350, 385
Mph1103I ATGCAT 1 cut(s) 62
MroXI GAANNNNTTC 2 cut(s) 416, 1089
MseI TTAA 5 cut(s) 399, 579, 596, 750, 996
MslI CAYNNNNRTG 1 cut(s) 80
MspA1I CMGCKG 1 cut(s) 727
MspI CCGG 3 cut(s) 252, 908, 1119
MspR9I CCNGG 6 cut(s) 253, 681, 1067, 1119, 1153, 1188
MvaI CCWGG 4 cut(s) 681, 1067, 1153, 1188
MvnI CGCG 2 cut(s) 376, 727
MwoI GCNNNNNNNGC 6 cut(s) 373, 636, 736, 923, 1025, 1034
NarI GGCGCC 1 cut(s) 1191
NciI CCSGG 2 cut(s) 253, 1119
NdeI CATATG 1 cut(s) 56
NdeII GATC 5 cut(s) 123, 423, 571, 1074, 1173
NlaIII CATG 6 cut(s) 85, 424, 485, 774, 847, 938
NlaIV GGNNCC 3 cut(s) 7, 836, 1192
NruI TCGCGA 1 cut(s) 376
NsbI TGCGCA 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 62
PagI TCATGA 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 1059
PdmI GAANNNNTTC 2 cut(s) 416, 1089
PfeI GAWTC 2 cut(s) 226, 313
PflFI GACNNNGTC 1 cut(s) 1061
PflMI CCANNNNNTGG 1 cut(s) 1213
PfoI TCCNGGA 1 cut(s) 1065
PkrI GCNGC 3 cut(s) 729, 975, 1030
PleI GAGTC 2 cut(s) 349, 385
PluTI GGCGCC 1 cut(s) 1194
PpsI GAGTC 2 cut(s) 349, 385
PpuMI RGGWCCY 1 cut(s) 834
PshAI GACNNNNGTC 2 cut(s) 681, 926
PshBI ATTAAT 1 cut(s) 996
Psp5II RGGWCCY 1 cut(s) 834
Psp6I CCWGG 4 cut(s) 679, 1065, 1151, 1186
PspEI GGTNACC 1 cut(s) 1160
PspGI CCWGG 4 cut(s) 679, 1065, 1151, 1186
PspN4I GGNNCC 3 cut(s) 7, 836, 1192
PspPI GGNCC 4 cut(s) 6, 200, 455, 834
PspPPI RGGWCCY 1 cut(s) 834
PsyI GACNNNGTC 1 cut(s) 1061
RruI TCGCGA 1 cut(s) 376
RsaI GTAC 2 cut(s) 803, 848
RsaNI GTAC 2 cut(s) 802, 847
RseI CAYNNNNRTG 1 cut(s) 80
SacII CCGCGG 1 cut(s) 728
SaqAI TTAA 5 cut(s) 399, 579, 596, 750, 996
SatI GCNGC 3 cut(s) 728, 974, 1029
Sau3AI GATC 5 cut(s) 123, 423, 571, 1074, 1173
Sau96I GGNCC 4 cut(s) 6, 200, 455, 834
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 2 cut(s) 350, 385
ScrFI CCNGG 6 cut(s) 253, 681, 1067, 1119, 1153, 1188
SduI GDGCHC 1 cut(s) 624
SfaNI GCATC 1 cut(s) 985
SfcI CTRYAG 1 cut(s) 186
SfoI GGCGCC 1 cut(s) 1192
Sfr303I CCGCGG 1 cut(s) 728
SgrBI CCGCGG 1 cut(s) 728
SinI GGWCC 4 cut(s) 6, 200, 455, 834
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 2 cut(s) 338, 564
SmoI CTYRAG 2 cut(s) 338, 564
Sse9I AATT 7 cut(s) 118, 246, 492, 610, 752, 1043, 1085
SsiI CCGC 5 cut(s) 9, 232, 554, 725, 727
SspDI GGCGCC 1 cut(s) 1190
SspMI CTAG 3 cut(s) 71, 144, 165
StyD4I CCNGG 6 cut(s) 251, 679, 1065, 1117, 1151, 1186
TaaI ACNGT 2 cut(s) 406, 892
TaqI TCGA 5 cut(s) 122, 224, 695, 1039, 1077
TasI AATT 7 cut(s) 118, 246, 492, 610, 752, 1043, 1085
TatI WGTACW 2 cut(s) 801, 846
TauI GCSGC 1 cut(s) 730
TfiI GAWTC 2 cut(s) 226, 313
Tru1I TTAA 5 cut(s) 399, 579, 596, 750, 996
Tru9I TTAA 5 cut(s) 399, 579, 596, 750, 996
TscAI CASTG 3 cut(s) 222, 895, 1259
TseI GCWGC 2 cut(s) 973, 1028
TspDTI ATGAA 5 cut(s) 98, 317, 409, 470, 604
TspRI CASTG 3 cut(s) 222, 895, 1259
Tth111I GACNNNGTC 1 cut(s) 1061
Van91I CCANNNNNTGG 1 cut(s) 1213
VpaK11BI GGWCC 4 cut(s) 6, 200, 455, 834
VspI ATTAAT 1 cut(s) 996
XapI RAATTY 2 cut(s) 610, 1043
XmnI GAANNNNTTC 2 cut(s) 416, 1089
XspI CTAG 3 cut(s) 71, 144, 165
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.