AT1G53920

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009749
Physical Location & Seq
Forward (+)
20137537 .. 20139716
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53920.1

Sequence Viewer

Length: 1158 bp
ATGAGAGAGTCAACTTTAATGGAGAAAGTCACAAGGAGAACTATCTCCTCCTTCATCTTCTTTATCGTCTCTTCTACCATTCTTTTTCTCGCCGGAAAATCCTCGGCCAAAATTTCTCATAACGGTGACAATAATGTGACGGCCTTGTTCCTCTTTGGAGACTCTTTCTTGGATGCAGGAAACAATAATTACATTAACACCACTACACTTGACCAAGCCAATTTTCCACCTTATGGTCAAACATTCTTTGGCCTCCCCACCGGAAGATTCTCCGACGGTCGTCTCATCTCTGATTTCATTGCGGAATATGCGAATTTGCCGTTAATTCCGCCGTTTTTGGAACCGGGAAATAGTCAAAAGAAGCTTTACGGCGTCAACTTTGCGTCTGCTGGTGCTGGTGCTCTTGTGGAAACGTTCCAAGGTTCGGTTATAAATTTGAGAACACAATTGGATCACTACAAGAAAGTGGAGAGATTATGGAGGACAAACTTTGGTAAAGAAGAAAGCAAGAAGAGAATCTCAAGAGCTGTCTATTTGATAAGCATTGGGAGCAATGACTACTCAAGCATTTTCTTGACCAATCAATCTCTTCCCATTTCAATGTCTCAACATGTTGACATTGTCATTGGTAATCTGACTACATTCATTCATGAAATATACAAGATTGGAGGACGTAAATTCGGGTTCTTGAATGTGCCTGACTTGGGTTGTTTTCCGGCTTTACGAATTCTACAACCGAAGAATGATGATTCTTGCTTAAGAGATGCTTCAAGATTAGCAAGCATGCATAACAGAGCTCTGACAAATCTCTTGTTTCAAATGCAAAGACAAGTCAAGGGATTCAAATTCTCCCTATTTGACATGAACAAAAGCCTTAGACTCAGAATGCAACATCCTTCTAAATTTGGGTTTAAAGAAGGGGAAGAAGCTTGTTGTGGAACTGGGAAGTGGAGAGGAGTATTCAGCTGTGGAGGGAAGAGAATTGTGAAAGAATATCAATTGTGTGAGAATCCTAAAGATTATATTTTTTGGGATTCACTTCATCTCACACAAAATACATATAACCAATTCGCAAACTTAATTTGGAACGGTGGACACATGTCAGATTCTCTAGTGGTTGGTCCATACAATATCAATAATTTATTTCAGATTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

385

Amino Acids

43.46

Weight (kDa)

9.32

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 49 - 361 2.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccB7I CCANNNNNTGG 1 cut(s) 233
AciI CCGC 2 cut(s) 302, 329
AclI AACGTT 1 cut(s) 413
AclWI GGATC 1 cut(s) 459
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 7 cut(s) 111, 313, 433, 677, 726, 845, 902
AcyI GRCGYC 1 cut(s) 372
AfiI CCNNNNNNNGG 3 cut(s) 233, 424, 704
AflII CTTAAG 1 cut(s) 757
AflIII ACRYGT 2 cut(s) 610, 1098
AgsI TTSAA 5 cut(s) 600, 691, 771, 818, 844
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AluBI AGCT 5 cut(s) 364, 527, 797, 929, 966
AluI AGCT 5 cut(s) 364, 527, 797, 929, 966
Alw21I GWGCWC 2 cut(s) 403, 799
Alw26I GTCTC 4 cut(s) 73, 153, 287, 609
AlwI GGATC 1 cut(s) 459
AoxI GGCC 3 cut(s) 105, 141, 250
ApoI RAATTY 7 cut(s) 111, 313, 433, 677, 726, 845, 902
AspS9I GGNCC 1 cut(s) 1121
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 137
AvaII GGWCC 1 cut(s) 1121
BanII GRGCYC 1 cut(s) 799
Bbv12I GWGCWC 2 cut(s) 403, 799
BceAI ACGGC 4 cut(s) 156, 304, 316, 385
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 4 cut(s) 73, 153, 287, 609
BfaI CTAG 1 cut(s) 1112
BfrI CTTAAG 1 cut(s) 757
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 1121
BmgT120I GGNCC 1 cut(s) 1121
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 951
BmsI GCATC 2 cut(s) 163, 754
BmuI ACTGGG 1 cut(s) 951
BoxI GACNNNNGTC 2 cut(s) 279, 1099
BpuEI CTTGAG 2 cut(s) 505, 547
BpuMI CCSGG 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 102, 418
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 424, 704
Bse1I ACTGG 1 cut(s) 946
Bse3DI GCAATG 2 cut(s) 297, 559
BseDI CCNNGG 2 cut(s) 102, 418
BseGI GGATG 2 cut(s) 178, 892
BseLI CCNNNNNNNGG 3 cut(s) 233, 424, 704
BseMI GCAATG 2 cut(s) 297, 559
BseMII CTCAG 1 cut(s) 895
BseNI ACTGG 1 cut(s) 946
BseRI GAGGAG 2 cut(s) 37, 969
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 107, 143, 252
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 403, 799
BsiSI CCGG 4 cut(s) 93, 261, 344, 716
BslI CCNNNNNNNGG 3 cut(s) 233, 424, 704
BsmAI GTCTC 4 cut(s) 73, 153, 287, 609
BsmBI CGTCTC 2 cut(s) 73, 287
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 3 cut(s) 107, 143, 252
Bsp1286I GDGCHC 2 cut(s) 403, 799
Bsp143I GATC 1 cut(s) 451
BspACI CCGC 2 cut(s) 302, 329
BspANI GGCC 3 cut(s) 107, 143, 252
BspCNI CTCAG 1 cut(s) 894
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 1 cut(s) 459
BspTI CTTAAG 1 cut(s) 757
BsrDI GCAATG 2 cut(s) 297, 559
BsrI ACTGG 1 cut(s) 946
BssECI CCNNGG 2 cut(s) 102, 418
BssMI GATC 1 cut(s) 451
BssNI GRCGYC 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 3 cut(s) 125, 278, 1091
Bst6I CTCTTC 4 cut(s) 76, 506, 594, 971
BstACI GRCGYC 1 cut(s) 372
BstAFI CTTAAG 1 cut(s) 757
BstC8I GCNNGC 2 cut(s) 781, 785
BstDEI CTNAG 2 cut(s) 875, 881
BstF5I GGATG 2 cut(s) 178, 892
BstKTI GATC 1 cut(s) 454
BstMAI GTCTC 4 cut(s) 73, 153, 287, 609
BstMBI GATC 1 cut(s) 451
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 308, 549
BstNSI RCATGY 3 cut(s) 614, 787, 1102
BstPAI GACNNNNGTC 2 cut(s) 279, 1099
BstSCI CCNGG 1 cut(s) 343
BsuRI GGCC 3 cut(s) 107, 143, 252
BtsCI GGATG 2 cut(s) 178, 892
Cac8I GCNNGC 2 cut(s) 781, 785
CciI TCATGA 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 1121
CseI GACGC 2 cut(s) 361, 372
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 5 cut(s) 611, 650, 784, 862, 1099
DdeI CTNAG 2 cut(s) 875, 881
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
DraI TTTAAA 1 cut(s) 913
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 4 cut(s) 76, 506, 594, 971
EarI CTCTTC 4 cut(s) 76, 506, 594, 971
EciI GGCGGA 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 797
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 1121
Eco53kI GAGCTC 1 cut(s) 797
EcoICRI GAGCTC 1 cut(s) 797
EcoRI GAATTC 1 cut(s) 726
EcoT14I CCWWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 789
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 418
Esp3I CGTCTC 2 cut(s) 73, 287
FaeI CATG 5 cut(s) 614, 653, 787, 865, 1102
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FatI CATG 5 cut(s) 610, 649, 783, 861, 1098
FokI GGATG 2 cut(s) 185, 879
FriOI GRGCYC 1 cut(s) 799
FspBI CTAG 1 cut(s) 1112
HaeIII GGCC 3 cut(s) 107, 143, 252
HapII CCGG 4 cut(s) 93, 261, 344, 716
HgaI GACGC 2 cut(s) 361, 372
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 5 cut(s) 614, 653, 787, 865, 1102
HincII GTYRAC 3 cut(s) 12, 376, 616
HindII GTYRAC 3 cut(s) 12, 376, 616
HindIII AAGCTT 2 cut(s) 362, 927
HpaII CCGG 4 cut(s) 93, 261, 344, 716
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 4 cut(s) 12, 376, 616, 1094
Hpy188I TCNGA 7 cut(s) 274, 292, 636, 801, 884, 1105, 1149
Hpy188III TCNNGA 5 cut(s) 522, 574, 650, 688, 771
Hpy8I GTNNAC 4 cut(s) 12, 376, 616, 1094
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 3 cut(s) 61, 906, 911
HpyCH4III ACNGT 3 cut(s) 125, 278, 1091
HpyCH4IV ACGT 2 cut(s) 413, 673
HpyCH4V TGCA 4 cut(s) 176, 787, 823, 889
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 549
HpyF3I CTNAG 2 cut(s) 875, 881
HpySE526I ACGT 2 cut(s) 413, 673
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 5 cut(s) 614, 653, 787, 865, 1102
Kzo9I GATC 1 cut(s) 451
LmnI GCTCC 1 cut(s) 549
LpnPI CCDG 9 cut(s) 106, 162, 274, 357, 375, 381, 711, 729, 927
LweI GCATC 2 cut(s) 163, 754
MaeI CTAG 1 cut(s) 1112
MaeII ACGT 2 cut(s) 413, 673
MaeIII GTNAC 3 cut(s) 28, 125, 136
MalI GATC 1 cut(s) 453
MboI GATC 1 cut(s) 451
MboII GAAGA 9 cut(s) 49, 63, 276, 512, 523, 581, 751, 935, 988
MfeI CAATTG 2 cut(s) 446, 998
MhlI GDGCHC 2 cut(s) 403, 799
MlyI GAGTC 3 cut(s) 17, 155, 873
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 8 cut(s) 58, 112, 161, 263, 474, 662, 947, 965
Mph1103I ATGCAT 1 cut(s) 789
MseI TTAA 6 cut(s) 17, 195, 323, 758, 912, 1079
MslI CAYNNNNRTG 2 cut(s) 123, 599
MspA1I CMGCKG 1 cut(s) 966
MspCI CTTAAG 1 cut(s) 757
MspI CCGG 4 cut(s) 93, 261, 344, 716
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 2 cut(s) 446, 998
Mva1269I GAATGC 1 cut(s) 891
MwoI GCNNNNNNNGC 2 cut(s) 308, 549
NciI CCSGG 1 cut(s) 345
NdeII GATC 1 cut(s) 451
NlaIII CATG 5 cut(s) 614, 653, 787, 865, 1102
NlaIV GGNNCC 1 cut(s) 342
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 3 cut(s) 28, 125, 136
NsiI ATGCAT 1 cut(s) 789
NspI RCATGY 3 cut(s) 614, 787, 1102
PaeI GCATGC 1 cut(s) 787
PagI TCATGA 1 cut(s) 649
PciI ACATGT 2 cut(s) 610, 1098
PctI GAATGC 1 cut(s) 891
PfeI GAWTC 8 cut(s) 267, 516, 749, 840, 1009, 1034, 1106, 1150
PflFI GACNNNGTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 233
PleI GAGTC 3 cut(s) 16, 155, 873
PpsI GAGTC 3 cut(s) 16, 155, 873
PscI ACATGT 2 cut(s) 610, 1098
PshAI GACNNNNGTC 2 cut(s) 279, 1099
PsiI TTATAA 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 799
Psp1406I AACGTT 1 cut(s) 413
PspN4I GGNNCC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 1121
PsyI GACNNNGTC 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 966
RseI CAYNNNNRTG 2 cut(s) 123, 599
SacI GAGCTC 1 cut(s) 799
SaqAI TTAA 6 cut(s) 17, 195, 323, 758, 912, 1079
Sau3AI GATC 1 cut(s) 451
Sau96I GGNCC 1 cut(s) 1121
SchI GAGTC 3 cut(s) 17, 155, 873
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 403, 799
SetI ASST 9 cut(s) 232, 366, 416, 424, 529, 676, 799, 931, 968
SfaNI GCATC 2 cut(s) 163, 754
SinI GGWCC 1 cut(s) 1121
SmiMI CAYNNNNRTG 2 cut(s) 123, 599
SmlI CTYRAG 3 cut(s) 520, 562, 757
SmoI CTYRAG 3 cut(s) 520, 562, 757
SphI GCATGC 1 cut(s) 787
SsiI CCGC 2 cut(s) 302, 329
SspMI CTAG 1 cut(s) 1112
SstI GAGCTC 1 cut(s) 799
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 3 cut(s) 125, 278, 1091
TaiI ACGT 2 cut(s) 416, 676
TfiI GAWTC 8 cut(s) 267, 516, 749, 840, 1009, 1034, 1106, 1150
Tru1I TTAA 6 cut(s) 17, 195, 323, 758, 912, 1079
Tru9I TTAA 6 cut(s) 17, 195, 323, 758, 912, 1079
TseFI GTSAC 3 cut(s) 28, 125, 136
Tsp45I GTSAC 3 cut(s) 28, 125, 136
TspDTI ATGAA 7 cut(s) 43, 286, 634, 638, 666, 878, 1031
Tth111I GACNNNGTC 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 757
VpaK11BI GGWCC 1 cut(s) 1121
XapI RAATTY 7 cut(s) 111, 313, 433, 677, 726, 845, 902
XceI RCATGY 3 cut(s) 614, 787, 1102
XspI CTAG 1 cut(s) 1112
Zsp2I ATGCAT 1 cut(s) 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.