Rorug02G0400400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009749
Physical Location & Seq
Reverse (-)
51099272 .. 51100369
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0400400.1

Sequence Viewer

Length: 693 bp
ATGGCAAGCTCTGCAATCCAGCAGTCAGCATTTGCCGGCCAGACCGCTTTGAAGCAGTCAAATGAGCTCATTCAGAAGATTGGCGGCCTTGGTGGTGGCCGCATCACCATGAGGCGCACCGTAAAAAGTGCCCCCGAGAACCGCCCAAAATACTTGGGACCATTCTCCGAGCAAACTCCATCATACTTGACTGGTGAATTCCCTGGTGACTATGGATGGGACACTGCTGGACTATCAGCAGACCCTGAGACCTTTGCCAAAAACCGTGAGCTTGAGTTTGGTTCAAGGCAGGGTCTCAAATCTTTTCAGAATTCAGGTGGCCTTGACTATCTTGGCAACCCAAACCTCGTCCATGCACAGAGCATCCTAGCAATCTGGGCTGTCCAGGTTGTGCTCATGGGATTTGTTGAAGGATACAGAGTTGGTGGAGGACCACTTGGTGAAGGTCTGGATTCACTTTACCCTGGAGGAGCCTATGATCCACTTGGATTGGCTGATGATCCTAAAGCCTTTGCTGAACTGAAGGTGAAGGAAATCAAGAACGGGCGGCTGGCAATGACCTCAATGTTTGGATTCTTTGTTCAGGCTATTGTAACTGGAAAGGGACCTGTTGAGAACCTCTTTGACCATGTTGCTGATCCAGTGGCTAACAATGCATGGGCTTATGCCACCAACTTCGTCCCCGGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

230

Amino Acids

24.54

Weight (kDa)

5.74

Isoelectric Point (pI)

29.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chloroa_b-bind PF00504 60 - 197 7.2e-29 Chlorophyll A-B binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 45, 84, 100, 142, 547
AclWI GGATC 3 cut(s) 473, 494, 632
AcoI YGGCCR 2 cut(s) 37, 97
AcsI RAATTY 2 cut(s) 197, 310
AcuI CTGAAG 1 cut(s) 542
AdeI CACNNNGTG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 52, 285, 410
AjnI CCWGG 3 cut(s) 202, 384, 463
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 3 cut(s) 9, 67, 271
AluI AGCT 3 cut(s) 9, 67, 271
Alw21I GWGCWC 2 cut(s) 69, 396
Alw26I GTCTC 2 cut(s) 242, 299
AlwI GGATC 3 cut(s) 473, 494, 632
AlwNI CAGNNNCTG 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 4 cut(s) 37, 85, 97, 319
ApoI RAATTY 2 cut(s) 197, 310
AspLEI GCGC 1 cut(s) 117
AspS9I GGNCC 3 cut(s) 158, 431, 605
AsuC2I CCSGG 1 cut(s) 684
AsuHPI GGTGA 5 cut(s) 97, 206, 218, 452, 538
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 3 cut(s) 158, 431, 605
BaeGI GKGCMC 1 cut(s) 133
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 69, 396
BccI CCATC 2 cut(s) 187, 210
BciT130I CCWGG 3 cut(s) 204, 386, 465
BciVI GTATCC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 684
BcoDI GTCTC 2 cut(s) 242, 299
BfaI CTAG 1 cut(s) 368
BfuI GTATCC 1 cut(s) 407
BisI GCNGC 3 cut(s) 85, 100, 548
BlsI GCNGC 3 cut(s) 86, 101, 549
Bme1390I CCNGG 4 cut(s) 204, 386, 465, 684
Bme18I GGWCC 3 cut(s) 158, 431, 605
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 3 cut(s) 158, 431, 605
BmiI GGNNCC 3 cut(s) 159, 472, 606
BmrFI CCNGG 4 cut(s) 204, 386, 465, 684
BmsI GCATC 2 cut(s) 111, 372
BpmI CTGGAG 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 684
BsaI GGTCTC 2 cut(s) 242, 299
BsaJI CCNNGG 4 cut(s) 88, 202, 463, 682
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 3 cut(s) 196, 601, 641
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 3 cut(s) 204, 386, 465
BseDI CCNNGG 4 cut(s) 88, 202, 463, 682
BseGI GGATG 2 cut(s) 221, 363
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 196, 601, 641
BseRI GAGGAG 1 cut(s) 483
BseSI GKGCMC 1 cut(s) 133
BshFI GGCC 4 cut(s) 39, 87, 99, 321
BsiHKAI GWGCWC 2 cut(s) 69, 396
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 2 cut(s) 36, 684
BslFI GGGAC 4 cut(s) 171, 233, 618, 665
BsmAI GTCTC 2 cut(s) 242, 299
BsmFI GGGAC 4 cut(s) 171, 233, 618, 665
BsnI GGCC 4 cut(s) 39, 87, 99, 321
Bso31I GGTCTC 2 cut(s) 242, 299
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 3 cut(s) 69, 133, 396
Bsp143I GATC 3 cut(s) 478, 499, 637
BspACI CCGC 5 cut(s) 45, 84, 100, 142, 547
BspANI GGCC 4 cut(s) 39, 87, 99, 321
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 3 cut(s) 159, 472, 606
BspPI GGATC 3 cut(s) 473, 494, 632
BspTNI GGTCTC 2 cut(s) 242, 299
BsrDI GCAATG 1 cut(s) 561
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 3 cut(s) 196, 601, 641
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 4 cut(s) 88, 202, 463, 682
BssMI GATC 3 cut(s) 478, 499, 637
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 3 cut(s) 204, 386, 465
Bst4CI ACNGT 2 cut(s) 121, 266
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 7, 37, 552
BstDEI CTNAG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 221, 363
BstHHI GCGC 1 cut(s) 117
BstKTI GATC 3 cut(s) 481, 502, 640
BstMAI GTCTC 2 cut(s) 242, 299
BstMBI GATC 3 cut(s) 478, 499, 637
BstMWI GCNNNNNNNGC 3 cut(s) 11, 377, 653
BstNI CCWGG 3 cut(s) 204, 386, 465
BstSCI CCNGG 4 cut(s) 202, 384, 463, 682
BstSLI GKGCMC 1 cut(s) 133
BsuI GTATCC 1 cut(s) 407
BsuRI GGCC 4 cut(s) 39, 87, 99, 321
BtsCI GGATG 2 cut(s) 221, 363
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 648
Cac8I GCNNGC 3 cut(s) 7, 37, 552
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 3 cut(s) 158, 431, 605
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviAII CATG 5 cut(s) 109, 353, 397, 629, 657
DdeI CTNAG 1 cut(s) 246
DpnI GATC 3 cut(s) 480, 501, 639
DpnII GATC 3 cut(s) 478, 499, 637
DraIII CACNNNGTG 1 cut(s) 440
EaeI YGGCCR 2 cut(s) 37, 97
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 2 cut(s) 242, 299
Eco47I GGWCC 3 cut(s) 158, 431, 605
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 605
EcoRI GAATTC 2 cut(s) 197, 310
EcoRII CCWGG 3 cut(s) 202, 384, 463
EcoT14I CCWWGG 1 cut(s) 88
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 5 cut(s) 112, 356, 400, 632, 660
FaiI YATR 9 cut(s) 110, 184, 213, 354, 398, 477, 630, 658, 666
FaqI GGGAC 4 cut(s) 171, 233, 618, 665
FatI CATG 5 cut(s) 108, 352, 396, 628, 656
Fnu4HI GCNGC 3 cut(s) 85, 100, 548
FokI GGATG 2 cut(s) 228, 350
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 3 cut(s) 85, 100, 548
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 116
GluI GCNGC 3 cut(s) 85, 100, 548
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 4 cut(s) 39, 87, 99, 321
HapII CCGG 2 cut(s) 36, 684
HhaI GCGC 1 cut(s) 117
Hin1II CATG 5 cut(s) 112, 356, 400, 632, 660
Hin6I GCGC 1 cut(s) 115
HinP1I GCGC 1 cut(s) 115
HinfI GANTC 2 cut(s) 452, 573
HpaII CCGG 2 cut(s) 36, 684
HphI GGTGA 5 cut(s) 97, 206, 218, 452, 538
Hpy188I TCNGA 3 cut(s) 75, 169, 309
Hpy188III TCNNGA 2 cut(s) 449, 538
HpyAV CCTTC 4 cut(s) 404, 437, 517, 523
HpyCH4III ACNGT 2 cut(s) 121, 266
HpyCH4V TGCA 3 cut(s) 14, 356, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 377, 653
HpyF3I CTNAG 1 cut(s) 246
Hsp92II CATG 5 cut(s) 112, 356, 400, 632, 660
HspAI GCGC 1 cut(s) 115
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 478, 499, 637
LmnI GCTCC 1 cut(s) 470
LweI GCATC 2 cut(s) 111, 372
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 206, 592
MalI GATC 3 cut(s) 480, 501, 639
MboI GATC 3 cut(s) 478, 499, 637
MboII GAAGA 1 cut(s) 88
MhlI GDGCHC 3 cut(s) 69, 133, 396
MluCI AATT 2 cut(s) 197, 310
MnlI CCTC 6 cut(s) 105, 356, 422, 461, 571, 629
Mph1103I ATGCAT 1 cut(s) 658
MroNI GCCGGC 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 2 cut(s) 36, 684
MspR9I CCNGG 4 cut(s) 204, 386, 465, 684
MvaI CCWGG 3 cut(s) 204, 386, 465
MwoI GCNNNNNNNGC 3 cut(s) 11, 377, 653
NaeI GCCGGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 684
NdeII GATC 3 cut(s) 478, 499, 637
NgoMIV GCCGGC 1 cut(s) 35
NlaIII CATG 5 cut(s) 112, 356, 400, 632, 660
NlaIV GGNNCC 3 cut(s) 159, 472, 606
NmuCI GTSAC 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 37
PfeI GAWTC 2 cut(s) 452, 573
PkrI GCNGC 3 cut(s) 86, 101, 549
PpuMI RGGWCCY 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 605
Psp6I CCWGG 3 cut(s) 202, 384, 463
PspGI CCWGG 3 cut(s) 202, 384, 463
PspN4I GGNNCC 3 cut(s) 159, 472, 606
PspPI GGNCC 3 cut(s) 158, 431, 605
PspPPI RGGWCCY 1 cut(s) 605
PstNI CAGNNNCTG 1 cut(s) 245
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 69
SatI GCNGC 3 cut(s) 85, 100, 548
Sau3AI GATC 3 cut(s) 478, 499, 637
Sau96I GGNCC 3 cut(s) 158, 431, 605
ScrFI CCNGG 4 cut(s) 204, 386, 465, 684
SduI GDGCHC 3 cut(s) 69, 133, 396
SfaNI GCATC 2 cut(s) 111, 372
SinI GGWCC 3 cut(s) 158, 431, 605
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 2 cut(s) 197, 310
SsiI CCGC 5 cut(s) 45, 84, 100, 142, 547
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 202, 384, 463, 682
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 2 cut(s) 121, 266
TasI AATT 2 cut(s) 197, 310
TauI GCSGC 3 cut(s) 87, 102, 550
TfiI GAWTC 2 cut(s) 452, 573
TscAI CASTG 2 cut(s) 229, 648
TseFI GTSAC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 206
TspRI CASTG 2 cut(s) 229, 648
VpaK11BI GGWCC 3 cut(s) 158, 431, 605
XapI RAATTY 2 cut(s) 197, 310
XspI CTAG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.