MD09G1174500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009749
Physical Location & Seq
Forward (+)
14804735 .. 14807598
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1174500.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAAAAGCAAAGCTCTGTTTCCGTCGTTTTGTTTCTTCATGCAGCACTACTGATCTCAACTTGTTTTGGAAGCAAATCCTGGTCTTCAAAAAAACAAGTTGCCTTGTTCATCTTCGGAGACTCTTACTTAGATTCTGGCAACAACAACTACATCAACACTACAACTCTCGACCAAGCAAATTTCTGGCCATATGGAGAAACCTACTTCAAGTTCCCAACTGGAAGATTCTCCGACGGACGTTTGATGTCAGACTTTATTGCTGAGTACGCTCACCTGCCATTTGTTCCACCCTTTCTTCAACCGGGATTTCGCCAATATTATGGTGGCGCGAATTTCGCATCTGCCGGAGCTGGAGCTCTGGTGGAGACTTTTCAAGGCGAGGTCATTAACCTTAAAACACAACTGAAGTATTACAAGAAGGTAGAGACCTGGTTCAGACACAAGTTAGGCGATGTTGAAGCCAAAGTGACACTTTCAAGAGGCGTCTACTTGTTCAACATTGGAACCAACGATTACATAAGCCCTTTCCTAACCAACTCTCCCATGTTAAAGTCCTACTCTCACTCACGATACGTCGAAATGGTTATCGGCAACTTGACGAGCGTCATCCAAGAAATTTATTCAGGAGGGGGTAGGAAATTTGGGGTCATCAATTTGCTGGACTCGGGTTGTCTTCCGGGGGTCAGAATAATCAAGCCTGAAAATAATGGTAGCTGCTTAAAAGAGGTTTCGTCACTTGCCAAATTGCATAACAAAGCTCTCTCCAAACTTCTCTCCAAACTTCTCATTCAACTTGGAAACCAGTTGGAGGGTTTCAAATATTCACTCTACGACTTGAACAGCAATCTTAGAAAGAGAATCAGGCATCCCTCTAAATATGGTTTTAAGGAAGGGAAAACAGCATGTTGCGGAACAGGTCAGTTTAGAGGAGTATTTAGCTGTGGAGGGAAAAGAACAGTGAAGAAGTTTGAGTTGTGTGAGGATCCAAATGAATATGTGTTTTGGGACTCTTTGCATCTCACTGAGAAGGTCTACAAGCAATTTGCTAATGAAATGTGGAGTGGCCGGCCATTGAACTCCAAGTCCACGACAGGTCTATACAGTCTCAAGGATCTCTTCCAAGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

42.46

Weight (kDa)

9.29

Isoelectric Point (pI)

30.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 352 6.9e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 285
AccI GTMKAC 2 cut(s) 489, 1035
AccII CGCG 1 cut(s) 332
AciI CCGC 1 cut(s) 912
AclWI GGATC 3 cut(s) 980, 993, 1122
AcoI YGGCCR 3 cut(s) 188, 1066, 1070
AcsI RAATTY 4 cut(s) 181, 334, 618, 641
AcuI CTGAAG 1 cut(s) 428
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 811
AjnI CCWGG 2 cut(s) 80, 431
AloI GAACNNNNNNTCC 2 cut(s) 1070, 1102
AluBI AGCT 6 cut(s) 15, 353, 359, 717, 761, 942
AluI AGCT 6 cut(s) 15, 353, 359, 717, 761, 942
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 4 cut(s) 114, 362, 422, 1112
AlwI GGATC 3 cut(s) 980, 993, 1122
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 3 cut(s) 188, 1066, 1070
ApeKI GCWGC 2 cut(s) 44, 717
ApoI RAATTY 4 cut(s) 181, 334, 618, 641
AspLEI GCGC 1 cut(s) 332
AsuC2I CCSGG 2 cut(s) 306, 681
AsuHPI GGTGA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 667
BalI TGGCCA 1 cut(s) 190
BamHI GGATCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 361
BbsI GAAGAC 2 cut(s) 78, 668
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 2 cut(s) 56, 704
BciT130I CCWGG 2 cut(s) 82, 433
BcnI CCSGG 2 cut(s) 306, 681
BcoDI GTCTC 4 cut(s) 114, 362, 422, 1112
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 2 cut(s) 45, 718
BlsI GCNGC 2 cut(s) 46, 719
Bme1390I CCNGG 4 cut(s) 82, 306, 433, 681
BmeT110I CYCGRG 1 cut(s) 667
BmiI GGNNCC 2 cut(s) 508, 987
BmrFI CCNGG 4 cut(s) 82, 306, 433, 681
BmsI GCATC 3 cut(s) 350, 877, 1027
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 2 cut(s) 78, 668
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 2 cut(s) 306, 681
BsaHI GRCGYC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 1 cut(s) 680
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 526, 556
Bsc4I CCNNNNNNNGG 1 cut(s) 811
Bse118I RCCGGY 1 cut(s) 1068
Bse1I ACTGG 2 cut(s) 226, 805
BseBI CCWGG 2 cut(s) 82, 433
BseDI CCNNGG 1 cut(s) 680
BseGI GGATG 2 cut(s) 609, 868
BseLI CCNNNNNNNGG 1 cut(s) 811
BseMII CTCAG 2 cut(s) 255, 1017
BseNI ACTGG 2 cut(s) 226, 805
BseRI GAGGAG 1 cut(s) 945
BseXI GCAGC 2 cut(s) 56, 704
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 3 cut(s) 190, 1068, 1072
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 4 cut(s) 305, 348, 680, 1069
BslFI GGGAC 1 cut(s) 1022
BslI CCNNNNNNNGG 1 cut(s) 811
BsmAI GTCTC 4 cut(s) 114, 362, 422, 1112
BsmFI GGGAC 1 cut(s) 1022
BsnI GGCC 3 cut(s) 190, 1068, 1072
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 667
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 3 cut(s) 54, 985, 1114
BspACI CCGC 1 cut(s) 912
BspANI GGCC 3 cut(s) 190, 1068, 1072
BspCNI CTCAG 2 cut(s) 256, 1018
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 2 cut(s) 508, 987
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 980, 993, 1122
BspTNI GGTCTC 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 1068
BsrI ACTGG 2 cut(s) 226, 805
BssAI RCCGGY 1 cut(s) 1068
BssECI CCNNGG 1 cut(s) 680
BssMI GATC 3 cut(s) 54, 985, 1114
BssNI GRCGYC 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 82, 433
Bst4CI ACNGT 2 cut(s) 961, 1106
Bst6I CTCTTC 1 cut(s) 1124
BstACI GRCGYC 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 1070
BstDEI CTNAG 4 cut(s) 130, 264, 851, 1026
BstF5I GGATG 2 cut(s) 609, 868
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 57, 988, 1117
BstMAI GTCTC 4 cut(s) 114, 362, 422, 1112
BstMBI GATC 3 cut(s) 54, 985, 1114
BstMWI GCNNNNNNNGC 2 cut(s) 269, 338
BstNI CCWGG 2 cut(s) 82, 433
BstNSI RCATGY 1 cut(s) 909
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 4 cut(s) 80, 304, 431, 679
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 56, 704
BstV2I GAAGAC 2 cut(s) 78, 668
BstX2I RGATCY 2 cut(s) 985, 1114
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 2 cut(s) 985, 1114
BsuRI GGCC 3 cut(s) 190, 1068, 1072
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 2 cut(s) 609, 868
BtsIMutI CAGTG 2 cut(s) 966, 1023
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 1070
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 1068
CseI GACGC 2 cut(s) 475, 595
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 1 cut(s) 268
CviAII CATG 3 cut(s) 41, 547, 906
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 4 cut(s) 130, 264, 851, 1026
DpnI GATC 3 cut(s) 56, 987, 1116
DpnII GATC 3 cut(s) 54, 985, 1114
EaeI YGGCCR 3 cut(s) 188, 1066, 1070
Eam1104I CTCTTC 1 cut(s) 1124
EarI CTCTTC 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 359
EcoRII CCWGG 2 cut(s) 80, 431
EcoT38I GRGCYC 1 cut(s) 361
FaeI CATG 3 cut(s) 44, 550, 909
FalI AAGNNNNNCTT 3 cut(s) 459, 491, 1103
FaqI GGGAC 1 cut(s) 1022
FatI CATG 3 cut(s) 40, 546, 905
FauNDI CATATG 1 cut(s) 193
FblI GTMKAC 2 cut(s) 489, 1035
Fnu4HI GCNGC 2 cut(s) 45, 718
FokI GGATG 2 cut(s) 596, 855
FriOI GRGCYC 1 cut(s) 361
FseI GGCCGGCC 1 cut(s) 1072
Fsp4HI GCNGC 2 cut(s) 45, 718
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 45, 718
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 3 cut(s) 190, 1068, 1072
HapII CCGG 4 cut(s) 305, 348, 680, 1069
HgaI GACGC 2 cut(s) 475, 595
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 3 cut(s) 44, 550, 909
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 6 cut(s) 122, 134, 228, 665, 861, 1010
HpaII CCGG 4 cut(s) 305, 348, 680, 1069
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 3 cut(s) 490, 1036, 1089
Hpy188I TCNGA 5 cut(s) 119, 235, 253, 440, 689
Hpy188III TCNNGA 4 cut(s) 170, 480, 570, 627
Hpy8I GTNNAC 3 cut(s) 490, 1036, 1089
Hpy99I CGWCG 3 cut(s) 29, 239, 581
HpyAV CCTTC 3 cut(s) 415, 887, 1024
HpyCH4III ACNGT 2 cut(s) 961, 1106
HpyCH4IV ACGT 2 cut(s) 241, 576
HpyCH4V TGCA 3 cut(s) 44, 751, 1018
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 338
HpyF3I CTNAG 4 cut(s) 130, 264, 851, 1026
HpySE526I ACGT 2 cut(s) 241, 576
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 3 cut(s) 44, 550, 909
HspAI GCGC 1 cut(s) 330
KroI GCCGGC 1 cut(s) 1068
KroNI GCCGGC 1 cut(s) 1070
Kzo9I GATC 3 cut(s) 54, 985, 1114
LmnI GCTCC 2 cut(s) 350, 356
Lsp1109I GCAGC 2 cut(s) 56, 704
LweI GCATC 3 cut(s) 350, 877, 1027
MabI ACCWGGT 1 cut(s) 431
MaeII ACGT 2 cut(s) 241, 576
MaeIII GTNAC 2 cut(s) 469, 735
MalI GATC 3 cut(s) 56, 987, 1116
MboI GATC 3 cut(s) 54, 985, 1114
MboII GAAGA 8 cut(s) 29, 78, 106, 237, 290, 668, 976, 1111
MflI RGATCY 2 cut(s) 985, 1114
MhlI GDGCHC 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1043
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 3 cut(s) 116, 659, 1004
MmeI TCCRAC 2 cut(s) 258, 789
MnlI CCTC 9 cut(s) 376, 476, 623, 721, 805, 883, 923, 941, 976
Mox20I TGGCCA 1 cut(s) 190
MroNI GCCGGC 1 cut(s) 1068
MscI TGGCCA 1 cut(s) 190
MseI TTAA 5 cut(s) 390, 396, 551, 722, 888
Msp20I TGGCCA 1 cut(s) 190
MspI CCGG 4 cut(s) 305, 348, 680, 1069
MspR9I CCNGG 4 cut(s) 82, 306, 433, 681
MvaI CCWGG 2 cut(s) 82, 433
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 2 cut(s) 269, 338
NaeI GCCGGC 1 cut(s) 1070
NciI CCSGG 2 cut(s) 306, 681
NdeI CATATG 1 cut(s) 193
NdeII GATC 3 cut(s) 54, 985, 1114
NgoMIV GCCGGC 1 cut(s) 1068
NlaIII CATG 3 cut(s) 44, 550, 909
NlaIV GGNNCC 2 cut(s) 508, 987
NmuCI GTSAC 2 cut(s) 469, 735
NspI RCATGY 1 cut(s) 909
PaqCI CACCTGC 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 1070
PfeI GAWTC 3 cut(s) 134, 228, 861
PkrI GCNGC 2 cut(s) 46, 719
PleI GAGTC 3 cut(s) 116, 659, 1004
PpsI GAGTC 3 cut(s) 116, 659, 1004
PshAI GACNNNNGTC 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 361
Psp6I CCWGG 2 cut(s) 80, 431
PspGI CCWGG 2 cut(s) 80, 431
PspN4I GGNNCC 2 cut(s) 508, 987
PsuI RGATCY 2 cut(s) 985, 1114
RigI GGCCGGCC 1 cut(s) 1072
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 361
SaqAI TTAA 5 cut(s) 390, 396, 551, 722, 888
SatI GCNGC 2 cut(s) 45, 718
Sau3AI GATC 3 cut(s) 54, 985, 1114
SchI GAGTC 3 cut(s) 116, 659, 1004
ScrFI CCNGG 4 cut(s) 82, 306, 433, 681
SduI GDGCHC 1 cut(s) 361
SexAI ACCWGGT 1 cut(s) 431
SfaNI GCATC 3 cut(s) 350, 877, 1027
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
Sse9I AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1043
SsiI CCGC 1 cut(s) 912
SspI AATATT 2 cut(s) 320, 824
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 4 cut(s) 80, 304, 431, 679
TaaI ACNGT 2 cut(s) 961, 1106
TaiI ACGT 2 cut(s) 244, 579
TaqI TCGA 2 cut(s) 171, 579
TasI AATT 7 cut(s) 181, 334, 618, 641, 655, 746, 1043
TfiI GAWTC 3 cut(s) 134, 228, 861
Tru1I TTAA 5 cut(s) 390, 396, 551, 722, 888
Tru9I TTAA 5 cut(s) 390, 396, 551, 722, 888
TscAI CASTG 2 cut(s) 966, 1030
TseFI GTSAC 2 cut(s) 469, 735
TseI GCWGC 2 cut(s) 44, 717
Tsp45I GTSAC 2 cut(s) 469, 735
TspDTI ATGAA 4 cut(s) 29, 100, 1008, 1068
TspGWI ACGGA 2 cut(s) 13, 252
TspRI CASTG 2 cut(s) 966, 1030
XapI RAATTY 4 cut(s) 181, 334, 618, 641
XceI RCATGY 1 cut(s) 909
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 489, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.