FvH4_6g35640

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009749
Physical Location & Seq
Reverse (-)
28125677 .. 28135131
9455 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35640.t1

Sequence Viewer

Length: 1143 bp
ATGGCAAGGCCGAGCTTTGTCTCCCTCCTTTTCTTCATTCATGCACTGCTAGTATTCACTAGAACTTGTTGTTTTGGATTAAGCAAATCCCAGCCACATTCAGAGAAGCCTCATCATCGCCATGGAGTTGCTTTCTTCATCTTCGGAGACTCTTACTTGGATGCAGGCAACAACAACTACATCAACACTTCAACTCTCGACCAGGCCAATTTCAGGCCTTATGGAGAGACATACTTCAAGTTCCCAACTGGAAGATTCTCCGACGGGCGCTTGATGTCGGACTTTATTGCCGAGTATGCTCACCGGCCATTGATTCCGCCGTTTCTGCAACCGGGGTTTCACCAGTATCCCGGCGGAGCCAACTTTGCATCTTCAGGAGCCGGTGCTCTGGTTGAGACGTTTCAAGGCTCGGTGATTGATCTAAAAACACAACTAAGAAATTACAAGAAGGTAGAGACCTGGTTCAGACACAAATTGGGCGATGCTGAAGCCAAGATGACACTGTCAAGAGCCGTCTACTTGTTTAACATCGGAACCAACGATTACATGAGCCCTTTCCTGATCGACTCCCCTATTTTCAAGTCCCACTCTCATTCCGAGTACGTAGGAATGGTCATCGGCAATTTGACGACTATCATCCAAGAGATTTATGCAGGAGGGGGTCGAAAATTTGGTTTCATCAATTTGCCGAGATTAGGTTGTCTCCCGGGTTTGAGAATAATCAAACCAGAAAATAACGGTAGCTGCTTAGAAGAAGTTTCATCACTTGCCAGATTACATAACCAAGCTCTCTTCAAAGTTCTCAGGAAGCTAGGAAGCCAGTTGAAGGGTTTTAAGTATTCACTCTACGACTTGAACCACAGCCTTAGACAGAGAACCAGACATCCTTCGAAATATGGTTTTAAAGAAGGAAAATCAGCATGCTGCGGAACAGGTCAGTTTCGAGGGGTGTTTAGCTGTGGAGGCAAGAGAATCGTCAAGGAGTTTGAGTTGTGCCAAAACCCTAATGAATACGTGTTTTGGGACTCTTTCCACTTGACTGACAAGGTTTATAGCCAATTGGCTCATGAAATGTGGAGTGGGGAAGGTGACTATAAGCCTTTGGGTCTGTACAATCTCAAGGATCTCTTCAGAGATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

381

Amino Acids

43.08

Weight (kDa)

9.23

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 357 1.4e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 516
AciI CCGC 3 cut(s) 317, 354, 927
AclWI GGATC 1 cut(s) 1131
AcoI YGGCCR 1 cut(s) 305
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 3 cut(s) 357, 507, 1114
AfaI GTAC 2 cut(s) 602, 1112
AfiI CCNNNNNNNGG 3 cut(s) 213, 695, 826
AflIII ACRYGT 1 cut(s) 1014
AgsI TTSAA 7 cut(s) 192, 238, 404, 580, 796, 826, 856
AjnI CCWGG 2 cut(s) 201, 458
AloI GAACNNNNNNTCC 2 cut(s) 868, 900
AluBI AGCT 5 cut(s) 15, 744, 788, 811, 957
AluI AGCT 5 cut(s) 15, 744, 788, 811, 957
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 6 cut(s) 25, 141, 221, 389, 449, 707
AlwI GGATC 1 cut(s) 1131
Ama87I CYCGRG 1 cut(s) 706
AoxI GGCC 4 cut(s) 8, 204, 215, 305
ApeKI GCWGC 2 cut(s) 744, 924
ApoI RAATTY 1 cut(s) 668
AspLEI GCGC 1 cut(s) 270
AsuC2I CCSGG 4 cut(s) 333, 351, 707, 708
AsuHPI GGTGA 4 cut(s) 293, 332, 424, 1100
AsuII TTCGAA 1 cut(s) 890
AvaI CYCGRG 1 cut(s) 706
BanII GRGCYC 1 cut(s) 554
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 731, 911
BceAI ACGGC 2 cut(s) 304, 497
BcgI CGANNNNNNTGC 2 cut(s) 955, 989
BciT130I CCWGG 2 cut(s) 203, 460
BciVI GTATCC 1 cut(s) 357
BcnI CCSGG 4 cut(s) 333, 351, 707, 708
BcoDI GTCTC 6 cut(s) 25, 141, 221, 389, 449, 707
BfaI CTAG 3 cut(s) 50, 60, 812
BfoI RGCGCY 1 cut(s) 271
BfuI GTATCC 1 cut(s) 357
BisI GCNGC 2 cut(s) 745, 925
BlsI GCNGC 2 cut(s) 746, 926
Bme1390I CCNGG 6 cut(s) 203, 333, 351, 460, 707, 708
BmeT110I CYCGRG 1 cut(s) 706
BmiI GGNNCC 3 cut(s) 358, 379, 535
BmrFI CCNGG 6 cut(s) 203, 333, 351, 460, 707, 708
BmsI GCATC 3 cut(s) 151, 377, 472
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 4 cut(s) 333, 351, 707, 708
BsaAI YACGTR 2 cut(s) 604, 1015
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 3 cut(s) 121, 332, 706
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 695, 826
Bse118I RCCGGY 2 cut(s) 303, 380
Bse1I ACTGG 3 cut(s) 253, 343, 820
BseBI CCWGG 2 cut(s) 203, 460
BseDI CCNNGG 3 cut(s) 121, 332, 706
BseGI GGATG 3 cut(s) 166, 636, 883
BseLI CCNNNNNNNGG 3 cut(s) 213, 695, 826
BseMII CTCAG 1 cut(s) 817
BseNI ACTGG 3 cut(s) 253, 343, 820
BseXI GCAGC 2 cut(s) 731, 911
BseYI CCCAGC 1 cut(s) 90
BshFI GGCC 4 cut(s) 10, 206, 217, 307
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 706
BsiSI CCGG 5 cut(s) 304, 332, 351, 381, 707
BslFI GGGAC 2 cut(s) 568, 1037
BslI CCNNNNNNNGG 3 cut(s) 213, 695, 826
BsmAI GTCTC 6 cut(s) 25, 141, 221, 389, 449, 707
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 2 cut(s) 568, 1037
BsnI GGCC 4 cut(s) 10, 206, 217, 307
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 706
Bsp119I TTCGAA 1 cut(s) 890
Bsp1286I GDGCHC 2 cut(s) 388, 554
Bsp1407I TGTACA 1 cut(s) 1110
Bsp143I GATC 3 cut(s) 418, 561, 1123
Bsp19I CCATGG 1 cut(s) 121
BspACI CCGC 3 cut(s) 317, 354, 927
BspANI GGCC 4 cut(s) 10, 206, 217, 307
BspCNI CTCAG 1 cut(s) 816
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 3 cut(s) 358, 379, 535
BspPI GGATC 1 cut(s) 1131
BspT104I TTCGAA 1 cut(s) 890
BspTNI GGTCTC 1 cut(s) 449
BsrFI RCCGGY 2 cut(s) 303, 380
BsrGI TGTACA 1 cut(s) 1110
BsrI ACTGG 3 cut(s) 253, 343, 820
BssAI RCCGGY 2 cut(s) 303, 380
BssECI CCNNGG 3 cut(s) 121, 332, 706
BssMI GATC 3 cut(s) 418, 561, 1123
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 203, 460
Bst4CI ACNGT 2 cut(s) 504, 740
Bst6I CTCTTC 2 cut(s) 797, 1133
BstAUI TGTACA 1 cut(s) 1110
BstBAI YACGTR 2 cut(s) 604, 1015
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 2 cut(s) 166, 922
BstDEI CTNAG 4 cut(s) 434, 748, 803, 866
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 3 cut(s) 166, 636, 883
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 3 cut(s) 421, 564, 1126
BstMAI GTCTC 6 cut(s) 25, 141, 221, 389, 449, 707
BstMBI GATC 3 cut(s) 418, 561, 1123
BstMWI GCNNNNNNNGC 4 cut(s) 296, 325, 365, 963
BstNI CCWGG 2 cut(s) 203, 460
BstNSI RCATGY 1 cut(s) 924
BstSCI CCNGG 6 cut(s) 201, 331, 349, 458, 705, 706
BstSNI TACGTA 1 cut(s) 604
BstV1I GCAGC 2 cut(s) 731, 911
BstX2I RGATCY 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 1123
BsuI GTATCC 1 cut(s) 357
BsuRI GGCC 4 cut(s) 10, 206, 217, 307
BtgI CCRYGG 1 cut(s) 121
BtgZI GCGATG 2 cut(s) 101, 495
BtsCI GGATG 3 cut(s) 166, 636, 883
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 500
Cac8I GCNNGC 2 cut(s) 166, 922
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 2 cut(s) 303, 380
Cfr9I CCCGGG 1 cut(s) 706
CsiI ACCWGGT 1 cut(s) 458
Csp6I GTAC 2 cut(s) 601, 1111
CviAII CATG 5 cut(s) 41, 122, 547, 921, 1067
CviQI GTAC 2 cut(s) 601, 1111
DdeI CTNAG 4 cut(s) 434, 748, 803, 866
DpnI GATC 3 cut(s) 420, 563, 1125
DpnII GATC 3 cut(s) 418, 561, 1123
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 797, 1133
EarI CTCTTC 2 cut(s) 797, 1133
EciI GGCGGA 2 cut(s) 306, 369
Eco105I TACGTA 1 cut(s) 604
Eco130I CCWWGG 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 554
Eco31I GGTCTC 1 cut(s) 449
Eco57I CTGAAG 3 cut(s) 357, 507, 1114
Eco88I CYCGRG 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 201, 458
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 5 cut(s) 44, 125, 550, 924, 1070
FalI AAGNNNNNCTT 1 cut(s) 1112
FaqI GGGAC 2 cut(s) 568, 1037
FatI CATG 5 cut(s) 40, 121, 546, 920, 1066
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 2 cut(s) 745, 925
FokI GGATG 3 cut(s) 173, 623, 870
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 2 cut(s) 745, 925
FspBI CTAG 3 cut(s) 50, 60, 812
GlaI GCGC 1 cut(s) 269
GluI GCNGC 2 cut(s) 745, 925
GsaI CCCAGC 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 4 cut(s) 10, 206, 217, 307
HapII CCGG 5 cut(s) 304, 332, 351, 381, 707
HhaI GCGC 1 cut(s) 270
Hin1II CATG 5 cut(s) 44, 125, 550, 924, 1070
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 6 cut(s) 149, 255, 313, 566, 972, 1025
HpaII CCGG 5 cut(s) 304, 332, 351, 381, 707
HphI GGTGA 4 cut(s) 293, 332, 424, 1100
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 8 cut(s) 103, 146, 262, 280, 467, 533, 598, 1133
Hpy188III TCNNGA 6 cut(s) 197, 375, 507, 559, 805, 1067
Hpy8I GTNNAC 1 cut(s) 517
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 5 cut(s) 442, 820, 897, 902, 1079
HpyCH4III ACNGT 2 cut(s) 504, 740
HpyCH4IV ACGT 3 cut(s) 398, 603, 1014
HpyCH4V TGCA 5 cut(s) 44, 164, 328, 368, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 325, 365, 963
HpyF3I CTNAG 4 cut(s) 434, 748, 803, 866
HpySE526I ACGT 3 cut(s) 398, 603, 1014
Hsp92II CATG 5 cut(s) 44, 125, 550, 924, 1070
HspAI GCGC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 418, 561, 1123
LmnI GCTCC 2 cut(s) 356, 377
Lsp1109I GCAGC 2 cut(s) 731, 911
LweI GCATC 3 cut(s) 151, 377, 472
MabI ACCWGGT 1 cut(s) 458
MaeI CTAG 3 cut(s) 50, 60, 812
MaeII ACGT 3 cut(s) 398, 603, 1014
MaeIII GTNAC 1 cut(s) 1088
MalI GATC 3 cut(s) 420, 563, 1125
MboI GATC 3 cut(s) 418, 561, 1123
MboII GAAGA 8 cut(s) 25, 127, 133, 264, 363, 764, 784, 1120
MfeI CAATTG 1 cut(s) 1058
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 2 cut(s) 388, 554
MluCI AATT 7 cut(s) 208, 439, 473, 622, 668, 682, 1058
MlyI GAGTC 3 cut(s) 143, 560, 1019
MmeI TCCRAC 2 cut(s) 258, 285
MnlI CCTC 5 cut(s) 35, 120, 650, 938, 956
MseI TTAA 4 cut(s) 80, 525, 834, 903
MslI CAYNNNNRTG 1 cut(s) 120
MspI CCGG 5 cut(s) 304, 332, 351, 381, 707
MspR9I CCNGG 6 cut(s) 203, 333, 351, 460, 707, 708
MunI CAATTG 1 cut(s) 1058
MvaI CCWGG 2 cut(s) 203, 460
MwoI GCNNNNNNNGC 4 cut(s) 296, 325, 365, 963
NciI CCSGG 4 cut(s) 333, 351, 707, 708
NcoI CCATGG 1 cut(s) 121
NdeII GATC 3 cut(s) 418, 561, 1123
NlaIII CATG 5 cut(s) 44, 125, 550, 924, 1070
NlaIV GGNNCC 3 cut(s) 358, 379, 535
NmeAIII GCCGAG 3 cut(s) 36, 316, 714
NmuCI GTSAC 1 cut(s) 1088
NspI RCATGY 1 cut(s) 924
NspV TTCGAA 1 cut(s) 890
PaeI GCATGC 1 cut(s) 924
PagI TCATGA 1 cut(s) 1066
PceI AGGCCT 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PfeI GAWTC 3 cut(s) 255, 313, 972
PflFI GACNNNGTC 1 cut(s) 502
PkrI GCNGC 2 cut(s) 746, 926
PleI GAGTC 3 cut(s) 143, 560, 1019
PpsI GAGTC 3 cut(s) 143, 560, 1019
Ppu21I YACGTR 2 cut(s) 604, 1015
Psp6I CCWGG 2 cut(s) 201, 458
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 201, 458
PspN4I GGNNCC 3 cut(s) 358, 379, 535
PsuI RGATCY 1 cut(s) 1123
PsyI GACNNNGTC 1 cut(s) 502
RsaI GTAC 2 cut(s) 602, 1112
RsaNI GTAC 2 cut(s) 601, 1111
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 4 cut(s) 80, 525, 834, 903
SatI GCNGC 2 cut(s) 745, 925
Sau3AI GATC 3 cut(s) 418, 561, 1123
SchI GAGTC 3 cut(s) 143, 560, 1019
ScrFI CCNGG 6 cut(s) 203, 333, 351, 460, 707, 708
SduI GDGCHC 2 cut(s) 388, 554
SexAI ACCWGGT 1 cut(s) 458
SfaNI GCATC 3 cut(s) 151, 377, 472
SfuI TTCGAA 1 cut(s) 890
SmaI CCCGGG 1 cut(s) 708
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 1118
SmoI CTYRAG 1 cut(s) 1118
SnaBI TACGTA 1 cut(s) 604
SphI GCATGC 1 cut(s) 924
Sse9I AATT 7 cut(s) 208, 439, 473, 622, 668, 682, 1058
SseBI AGGCCT 1 cut(s) 217
SsiI CCGC 3 cut(s) 317, 354, 927
SspMI CTAG 3 cut(s) 50, 60, 812
StuI AGGCCT 1 cut(s) 217
StyD4I CCNGG 6 cut(s) 201, 331, 349, 458, 705, 706
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 2 cut(s) 504, 740
TaiI ACGT 3 cut(s) 401, 606, 1017
TaqI TCGA 5 cut(s) 198, 564, 664, 890, 943
TasI AATT 7 cut(s) 208, 439, 473, 622, 668, 682, 1058
TatI WGTACW 1 cut(s) 1110
TfiI GAWTC 3 cut(s) 255, 313, 972
Tru1I TTAA 4 cut(s) 80, 525, 834, 903
Tru9I TTAA 4 cut(s) 80, 525, 834, 903
TscAI CASTG 2 cut(s) 51, 507
TseFI GTSAC 1 cut(s) 1088
TseI GCWGC 2 cut(s) 744, 924
Tsp45I GTSAC 1 cut(s) 1088
TspDTI ATGAA 7 cut(s) 25, 29, 127, 667, 750, 1023, 1083
TspMI CCCGGG 1 cut(s) 706
TspRI CASTG 2 cut(s) 51, 507
Tth111I GACNNNGTC 1 cut(s) 502
XapI RAATTY 1 cut(s) 668
XceI RCATGY 1 cut(s) 924
XmaI CCCGGG 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 516
XspI CTAG 3 cut(s) 50, 60, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.