MD17G1152100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009749
Physical Location & Seq
Reverse (-)
14070617 .. 14073883
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1152100.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGGCAAAGCAAAGCTTTATCTCCCTCGTTTTCTTTCTCCATGCAGCACTACTAATCTCAACTTGTTTTGGAAGCAAATCTTGGCTTTCAAAAAGGCAAGTTGCCTTGTTCATCTTTGGAGACTCTTACTTAGATTCTGGCAACAACAACTACATCAACACTACAACTCTCGACCAAGCAAATTTCTTGCCGTACGGAGAAACCTACTTCAAGTTCCCAACGGGAAGATTCTCCGACGGACGTTTGATGTCAGACTTCATTGCTGAGTACGCTCACCTGCCATTTGTTCCACCCTTTCTTCAACCGGGATTTCACCAATATTATGGTGGTGCGAATTTCGCATCCGCCGGAGCTGGTGCTCTTGTTGAGACTTTTCATGGCGAGGTCATTGATCTTAAGACACAACTGAAGTACTATAAGAAGGTAGAGACCTGGTTCAGACACAAGTTAGGCAATGTTGAAGCCAAAGTGACACTTTCAAGAGCCGTCTACTTGTTCAGCATTGGAACCAACGATTACATAAGCCCTTTCCTAACCAATTCTCCCATGTTAAAGTCCTACCCTCGCTCACAATACGTCGGAATGGTCATCGGCAACTTGACAAGCGTTATCCAAGAAATTTATGCAGGAGGGGGTAGGAAATTTGGGTTCATCAACTTGCTGGACTCGGGTTGTCTTCCGGGGGTCAGAATAATCAAGCCTGAAAATAATGGTAGCTGCTTAAAAGAGGTTTCATCACTTGCCAAATTACATAACCAAGCACTCTCAAAACTTCTCATTCAACTTGGAAACCAGTTGGAGGGTTTCAAATATTCACTCTACGACTCGAACAGCAATCTTAGACAGAGAATCAGACATCCCTCAAAATATGGTTTTAAAGAAGGGAAAACAGCATGCTGCGGGACAGGTGAATTTAGAGGAGTGTTTAGCTGTGGAGGGAAAAGAATAGTGAATGAGTTTGAATTGTGTGAAAATCCAAATGAATATGTGTTTTGGGACTCATTGCATCTCACTGAAAAGGTCTACAAGCAATTTGCTAATGAAATGTGGGGTGGCCAGCCATGGAACTCCAAGGCCACAAAAGGTCTAAACAATCTCAAGAGTCTGTTCCAAGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

41.95

Weight (kDa)

9.1

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 348 1.5e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 285
AccI GTMKAC 2 cut(s) 489, 1023
AciI CCGC 2 cut(s) 345, 900
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 5 cut(s) 181, 334, 618, 641, 911
AcuI CTGAAG 1 cut(s) 428
AfaI GTAC 3 cut(s) 194, 269, 413
AfiI CCNNNNNNNGG 1 cut(s) 799
AflII CTTAAG 1 cut(s) 395
AgsI TTSAA 8 cut(s) 90, 211, 302, 461, 480, 782, 808, 962
AjnI CCWGG 1 cut(s) 431
AjuI GAANNNNNNNTTGG 2 cut(s) 64, 96
AluBI AGCT 4 cut(s) 15, 353, 717, 930
AluI AGCT 4 cut(s) 15, 353, 717, 930
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 3 cut(s) 114, 362, 422
Ama87I CYCGRG 1 cut(s) 667
AoxI GGCC 2 cut(s) 1054, 1074
ApeKI GCWGC 3 cut(s) 44, 717, 897
ApoI RAATTY 5 cut(s) 181, 334, 618, 641, 911
AsuC2I CCSGG 2 cut(s) 306, 681
AsuHPI GGTGA 3 cut(s) 266, 305, 920
AvaI CYCGRG 1 cut(s) 667
BalI TGGCCA 1 cut(s) 1056
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 3 cut(s) 56, 704, 884
BceAI ACGGC 2 cut(s) 175, 470
BciT130I CCWGG 1 cut(s) 433
BcnI CCSGG 2 cut(s) 306, 681
BcoDI GTCTC 3 cut(s) 114, 362, 422
BfrI CTTAAG 1 cut(s) 395
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 3 cut(s) 45, 718, 898
BlsI GCNGC 3 cut(s) 46, 719, 899
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 3 cut(s) 306, 433, 681
BmeT110I CYCGRG 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 3 cut(s) 306, 433, 681
BmsI GCATC 2 cut(s) 350, 1015
BpiI GAAGAC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 2 cut(s) 306, 681
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 3 cut(s) 680, 1061, 1071
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 1 cut(s) 799
Bse1I ACTGG 1 cut(s) 793
Bse3DI GCAATG 3 cut(s) 258, 460, 1001
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 3 cut(s) 680, 1061, 1071
BseGI GGATG 2 cut(s) 341, 856
BseLI CCNNNNNNNGG 1 cut(s) 799
BseMI GCAATG 3 cut(s) 258, 460, 1001
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 1 cut(s) 933
BseXI GCAGC 3 cut(s) 56, 704, 884
BshFI GGCC 2 cut(s) 1056, 1076
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 667
BsiSI CCGG 3 cut(s) 305, 348, 680
BsiWI CGTACG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 916, 1010
BslI CCNNNNNNNGG 1 cut(s) 799
BsmAI GTCTC 3 cut(s) 114, 362, 422
BsmFI GGGAC 2 cut(s) 916, 1010
BsnI GGCC 2 cut(s) 1056, 1076
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 667
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 1061
BspACI CCGC 2 cut(s) 345, 900
BspANI GGCC 2 cut(s) 1056, 1076
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 508
BspMI ACCTGC 1 cut(s) 285
BspTI CTTAAG 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 422
BsrDI GCAATG 3 cut(s) 258, 460, 1001
BsrI ACTGG 1 cut(s) 793
BssECI CCNNGG 3 cut(s) 680, 1061, 1071
BssMI GATC 1 cut(s) 391
BssT1I CCWWGG 2 cut(s) 1061, 1071
Bst2UI CCWGG 1 cut(s) 433
BstAFI CTTAAG 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 895, 1058
BstDEI CTNAG 3 cut(s) 130, 264, 839
BstDSI CCRYGG 1 cut(s) 1061
BstF5I GGATG 2 cut(s) 341, 856
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 3 cut(s) 114, 362, 422
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 269, 338
BstNI CCWGG 1 cut(s) 433
BstNSI RCATGY 1 cut(s) 897
BstSCI CCNGG 3 cut(s) 304, 431, 679
BstV1I GCAGC 3 cut(s) 56, 704, 884
BstV2I GAAGAC 1 cut(s) 668
BstXI CCANNNNNNTGG 1 cut(s) 323
BsuRI GGCC 2 cut(s) 1056, 1076
BtgI CCRYGG 1 cut(s) 1061
BtsCI GGATG 2 cut(s) 341, 856
BtsIMutI CAGTG 1 cut(s) 1011
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 895, 1058
CsiI ACCWGGT 1 cut(s) 431
Csp6I GTAC 3 cut(s) 193, 268, 412
CviAII CATG 5 cut(s) 41, 377, 547, 894, 1062
CviQI GTAC 3 cut(s) 193, 268, 412
DdeI CTNAG 3 cut(s) 130, 264, 839
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
DraI TTTAAA 1 cut(s) 877
EaeI YGGCCR 1 cut(s) 1054
EciI GGCGGA 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 1061, 1071
Eco31I GGTCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 2 cut(s) 1061, 1071
ErhI CCWWGG 2 cut(s) 1061, 1071
FaeI CATG 5 cut(s) 44, 380, 550, 897, 1065
FalI AAGNNNNNCTT 5 cut(s) 31, 64, 96, 459, 491
FaqI GGGAC 2 cut(s) 916, 1010
FatI CATG 5 cut(s) 40, 376, 546, 893, 1061
FauI CCCGC 1 cut(s) 893
FblI GTMKAC 2 cut(s) 489, 1023
Fnu4HI GCNGC 3 cut(s) 45, 718, 898
FokI GGATG 2 cut(s) 328, 843
Fsp4HI GCNGC 3 cut(s) 45, 718, 898
GluI GCNGC 3 cut(s) 45, 718, 898
HaeIII GGCC 2 cut(s) 1056, 1076
HapII CCGG 3 cut(s) 305, 348, 680
Hin1II CATG 5 cut(s) 44, 380, 550, 897, 1065
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 8 cut(s) 122, 134, 228, 665, 824, 849, 998, 1102
HpaII CCGG 3 cut(s) 305, 348, 680
HphI GGTGA 3 cut(s) 266, 305, 920
Hpy166II GTNNAC 2 cut(s) 490, 1024
Hpy188I TCNGA 6 cut(s) 235, 253, 440, 581, 689, 854
Hpy188III TCNNGA 3 cut(s) 170, 480, 1099
Hpy8I GTNNAC 2 cut(s) 490, 1024
Hpy99I CGWCG 2 cut(s) 239, 581
HpyAV CCTTC 2 cut(s) 415, 875
HpyCH4IV ACGT 2 cut(s) 241, 576
HpyCH4V TGCA 3 cut(s) 44, 626, 1006
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 338
HpyF3I CTNAG 3 cut(s) 130, 264, 839
HpySE526I ACGT 2 cut(s) 241, 576
Hsp92II CATG 5 cut(s) 44, 380, 550, 897, 1065
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 350
Lsp1109I GCAGC 3 cut(s) 56, 704, 884
LweI GCATC 2 cut(s) 350, 1015
MabI ACCWGGT 1 cut(s) 431
MaeII ACGT 2 cut(s) 241, 576
MaeIII GTNAC 1 cut(s) 469
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 237, 290, 668
MhlI GDGCHC 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 1056
MluCI AATT 9 cut(s) 181, 334, 538, 618, 641, 746, 911, 962, 1031
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 5 cut(s) 116, 659, 818, 992, 1111
MmeI TCCRAC 3 cut(s) 258, 559, 777
MnlI CCTC 9 cut(s) 35, 376, 573, 623, 721, 793, 871, 911, 929
Mox20I TGGCCA 1 cut(s) 1056
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 4 cut(s) 396, 551, 722, 876
Msp20I TGGCCA 1 cut(s) 1056
MspCI CTTAAG 1 cut(s) 395
MspI CCGG 3 cut(s) 305, 348, 680
MspR9I CCNGG 3 cut(s) 306, 433, 681
MvaI CCWGG 1 cut(s) 433
MwoI GCNNNNNNNGC 2 cut(s) 269, 338
NciI CCSGG 2 cut(s) 306, 681
NcoI CCATGG 1 cut(s) 1061
NdeII GATC 1 cut(s) 391
NlaIII CATG 5 cut(s) 44, 380, 550, 897, 1065
NlaIV GGNNCC 1 cut(s) 508
NmuCI GTSAC 1 cut(s) 469
NspI RCATGY 1 cut(s) 897
PaeI GCATGC 1 cut(s) 897
PaqCI CACCTGC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 134, 228, 849
Pfl23II CGTACG 1 cut(s) 192
PkrI GCNGC 3 cut(s) 46, 719, 899
PleI GAGTC 5 cut(s) 116, 659, 818, 992, 1110
PpsI GAGTC 5 cut(s) 116, 659, 818, 992, 1110
Psp6I CCWGG 1 cut(s) 431
PspGI CCWGG 1 cut(s) 431
PspLI CGTACG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 508
RsaI GTAC 3 cut(s) 194, 269, 413
RsaNI GTAC 3 cut(s) 193, 268, 412
SaqAI TTAA 4 cut(s) 396, 551, 722, 876
SatI GCNGC 3 cut(s) 45, 718, 898
Sau3AI GATC 1 cut(s) 391
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 5 cut(s) 116, 659, 818, 992, 1111
ScrFI CCNGG 3 cut(s) 306, 433, 681
SduI GDGCHC 1 cut(s) 361
SexAI ACCWGGT 1 cut(s) 431
SfaNI GCATC 2 cut(s) 350, 1015
SmlI CTYRAG 2 cut(s) 395, 1097
SmoI CTYRAG 2 cut(s) 395, 1097
SphI GCATGC 1 cut(s) 897
Sse9I AATT 9 cut(s) 181, 334, 538, 618, 641, 746, 911, 962, 1031
SsiI CCGC 2 cut(s) 345, 900
SspI AATATT 2 cut(s) 320, 812
StyD4I CCNGG 3 cut(s) 304, 431, 679
StyI CCWWGG 2 cut(s) 1061, 1071
TaiI ACGT 2 cut(s) 244, 579
TaqI TCGA 2 cut(s) 171, 827
TasI AATT 9 cut(s) 181, 334, 538, 618, 641, 746, 911, 962, 1031
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 3 cut(s) 134, 228, 849
Tru1I TTAA 4 cut(s) 396, 551, 722, 876
Tru9I TTAA 4 cut(s) 396, 551, 722, 876
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 3 cut(s) 44, 717, 897
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 7 cut(s) 100, 247, 365, 640, 723, 996, 1056
TspGWI ACGGA 2 cut(s) 210, 252
TspRI CASTG 1 cut(s) 1018
Vha464I CTTAAG 1 cut(s) 395
XapI RAATTY 5 cut(s) 181, 334, 618, 641, 911
XceI RCATGY 1 cut(s) 897
XcmI CCANNNNNNNNNTGG 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 489, 1023
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.