RLG00000020265

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009749
Physical Location & Seq
Reverse (-)
63030084 .. 63032275
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020265

Sequence Viewer

Length: 759 bp
ATGTTCACATGCTCAATGATTTCTGAGGTGATTGATCTGAAAACACAACTAAGAAATTACAAGAAGGTAGAGACCTGGTTCAGACACAAGTTGGGCGATGCTGAAGCCAAAATGACACTGTCAAGAGCCGTCTACTTGTTTAACATCGGAACCAACGATTACATGAGCCCTTTCTTGATCGACTCCCCCATTTTCAAGTCCCACTCTCACTCAGAATACGTTGGAATGGTCATTGGCAATTTGACAACTATCATCCAAGAGATTTATGCAGGAGGGGGTCGAAAATTTGGGTTCATCAACTTGTTGAGATTAGGTTGTCTTCCGGGCTTGAGAATACTCAAACAAGAAAACAATGGAAGCTGCTTAGAAGAAGTTTCATCACTTGCCAGATTACATAACCAAGCTCTCTCGAAAGTTCTCAGGAAACTAGGAAGTCAGTTGAAGGGTTTTAAATATTCACTCTACGACTTGAACCACAACCTTAGACAGAGAACCAGACATCCTTCTAAATATGGTTTTAAAGAAGGAAAATCAGCATGCTGCGGAACAGGTCAGTTTCGAGGGGTGTTCAGCTGTGGAGGGAAGAGAATCTTGAAGGAGTTTGAGTTGTGCGAAAACCCTAGTGAATATCTGTTTTGGGACTCTTTCCATCTGACTGAAAAAGTTTATAGCCAATTAGCTTATCAAATGTGGAGTGGGGAAGATGACTCTAAGCCCTTGGGTCTATACAATCTGAAGGATCTCTTCAGAGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

253

Amino Acids

28.92

Weight (kDa)

9.14

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 228 2e-18 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 132
AciI CCGC 1 cut(s) 543
AclWI GGATC 1 cut(s) 747
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 3 cut(s) 123, 730, 755
AgsI TTSAA 4 cut(s) 196, 442, 472, 595
AjnI CCWGG 1 cut(s) 74
AloI GAACNNNNNNTCC 2 cut(s) 484, 516
AluBI AGCT 4 cut(s) 360, 404, 573, 680
AluI AGCT 4 cut(s) 360, 404, 573, 680
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 747
ApeKI GCWGC 2 cut(s) 360, 540
ApoI RAATTY 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 40
BanII GRGCYC 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 347, 527
BccI CCATC 1 cut(s) 657
BceAI ACGGC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 76
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 3 cut(s) 428, 621, 757
BisI GCNGC 2 cut(s) 361, 541
BlsI GCNGC 2 cut(s) 362, 542
Bme1390I CCNGG 2 cut(s) 76, 324
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 76, 324
BmsI GCATC 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 349
BpuMI CCSGG 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 717
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 717
BseGI GGATG 2 cut(s) 252, 499
BseMII CTCAG 3 cut(s) 15, 225, 433
BseXI GCAGC 2 cut(s) 347, 527
BsiSI CCGG 1 cut(s) 323
BslFI GGGAC 2 cut(s) 184, 653
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 184, 653
Bso31I GGTCTC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 3 cut(s) 34, 177, 739
BspACI CCGC 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 16, 224, 432
BspLI GGNNCC 1 cut(s) 151
BspPI GGATC 1 cut(s) 747
BspTNI GGTCTC 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 717
BssMI GATC 3 cut(s) 34, 177, 739
BssT1I CCWWGG 1 cut(s) 717
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 1 cut(s) 120
Bst6I CTCTTC 2 cut(s) 578, 749
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 7 cut(s) 24, 50, 211, 364, 419, 482, 711
BstF5I GGATG 2 cut(s) 252, 499
BstKTI GATC 3 cut(s) 37, 180, 742
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 3 cut(s) 34, 177, 739
BstNI CCWGG 1 cut(s) 76
BstNSI RCATGY 2 cut(s) 12, 540
BstSCI CCNGG 2 cut(s) 74, 322
BstV1I GCAGC 2 cut(s) 347, 527
BstV2I GAAGAC 1 cut(s) 311
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 252, 499
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 538
CsiI ACCWGGT 1 cut(s) 74
CviAII CATG 3 cut(s) 9, 163, 537
DdeI CTNAG 7 cut(s) 24, 50, 211, 364, 419, 482, 711
DpnI GATC 3 cut(s) 36, 179, 741
DpnII GATC 3 cut(s) 34, 177, 739
DraI TTTAAA 2 cut(s) 451, 520
Eam1104I CTCTTC 2 cut(s) 578, 749
EarI CTCTTC 2 cut(s) 578, 749
Eco130I CCWWGG 1 cut(s) 717
Eco24I GRGCYC 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 65
Eco57I CTGAAG 3 cut(s) 123, 730, 755
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 717
FaeI CATG 3 cut(s) 12, 166, 540
FaiI YATR 8 cut(s) 10, 164, 267, 396, 513, 538, 669, 727
FalI AAGNNNNNCTT 3 cut(s) 575, 607, 728
FaqI GGGAC 2 cut(s) 184, 653
FatI CATG 3 cut(s) 8, 162, 536
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 361, 541
FokI GGATG 2 cut(s) 239, 486
FriOI GRGCYC 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 361, 541
FspBI CTAG 3 cut(s) 428, 621, 757
GluI GCNGC 2 cut(s) 361, 541
HapII CCGG 1 cut(s) 323
Hin1II CATG 3 cut(s) 12, 166, 540
HinfI GANTC 4 cut(s) 182, 588, 641, 707
HpaII CCGG 1 cut(s) 323
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 2 cut(s) 6, 133
Hpy188I TCNGA 8 cut(s) 25, 39, 83, 149, 214, 654, 735, 749
Hpy188III TCNNGA 5 cut(s) 123, 175, 409, 421, 592
Hpy8I GTNNAC 2 cut(s) 6, 133
HpyAV CCTTC 6 cut(s) 58, 436, 513, 518, 589, 730
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 269
HpyF3I CTNAG 7 cut(s) 24, 50, 211, 364, 419, 482, 711
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 3 cut(s) 12, 166, 540
Kzo9I GATC 3 cut(s) 34, 177, 739
LpnPI CCDG 8 cut(s) 61, 88, 255, 336, 400, 406, 508, 534
Lsp1109I GCAGC 2 cut(s) 347, 527
LweI GCATC 1 cut(s) 88
MabI ACCWGGT 1 cut(s) 74
MaeI CTAG 3 cut(s) 428, 621, 757
MaeII ACGT 1 cut(s) 219
MalI GATC 3 cut(s) 36, 179, 741
MboI GATC 3 cut(s) 34, 177, 739
MboII GAAGA 5 cut(s) 311, 380, 595, 713, 736
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 4 cut(s) 55, 238, 284, 674
MlyI GAGTC 3 cut(s) 176, 635, 701
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 4 cut(s) 19, 266, 554, 572
MseI TTAA 3 cut(s) 141, 450, 519
MspA1I CMGCKG 1 cut(s) 573
MspI CCGG 1 cut(s) 323
MspR9I CCNGG 2 cut(s) 76, 324
MvaI CCWGG 1 cut(s) 76
NciI CCSGG 1 cut(s) 324
NdeII GATC 3 cut(s) 34, 177, 739
NlaIII CATG 3 cut(s) 12, 166, 540
NlaIV GGNNCC 1 cut(s) 151
NspI RCATGY 2 cut(s) 12, 540
PaeI GCATGC 1 cut(s) 540
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PfeI GAWTC 1 cut(s) 588
PflFI GACNNNGTC 1 cut(s) 118
PkrI GCNGC 2 cut(s) 362, 542
PleI GAGTC 3 cut(s) 176, 635, 701
PpsI GAGTC 3 cut(s) 176, 635, 701
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 151
PsuI RGATCY 1 cut(s) 739
PsyI GACNNNGTC 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 573
SaqAI TTAA 3 cut(s) 141, 450, 519
SatI GCNGC 2 cut(s) 361, 541
Sau3AI GATC 3 cut(s) 34, 177, 739
SchI GAGTC 3 cut(s) 176, 635, 701
ScrFI CCNGG 2 cut(s) 76, 324
SduI GDGCHC 1 cut(s) 170
SexAI ACCWGGT 1 cut(s) 74
SfaNI GCATC 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SphI GCATGC 1 cut(s) 540
Sse9I AATT 4 cut(s) 55, 238, 284, 674
SsiI CCGC 1 cut(s) 543
SspI AATATT 1 cut(s) 455
SspMI CTAG 3 cut(s) 428, 621, 757
StyD4I CCNGG 2 cut(s) 74, 322
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 1 cut(s) 120
TaiI ACGT 1 cut(s) 222
TaqI TCGA 4 cut(s) 180, 280, 410, 559
TasI AATT 4 cut(s) 55, 238, 284, 674
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 3 cut(s) 141, 450, 519
Tru9I TTAA 3 cut(s) 141, 450, 519
TscAI CASTG 1 cut(s) 123
TseI GCWGC 2 cut(s) 360, 540
TspDTI ATGAA 2 cut(s) 283, 366
TspRI CASTG 1 cut(s) 123
Tth111I GACNNNGTC 1 cut(s) 118
XapI RAATTY 1 cut(s) 284
XceI RCATGY 2 cut(s) 12, 540
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 3 cut(s) 428, 621, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.