Rh2AG457800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009749
Physical Location & Seq
Reverse (-)
67318945 .. 67322002
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG457800.1

Sequence Viewer

Length: 1140 bp
ATGGCAAGGCAAAGCTTTGTCTACCTCCTTTTCTTTCTTCATGTACTACTAATATTTTCAAGTAGTTGCTTTGGATTAAGCAAATCCCAGCATTCAGAGAAGCCGCATCATCATCATCATGAAGTTGCTTTCTTCATCTTCGGAGACTCCTACTTGGATGCAGGCAACAACAACTACATCAACACTTCAACTCTTGACCAGGCCAATTTCAGGCCTTATGGAGAGACCTACTTCAAGTTCCCAACTGGAAGATTCTCCGACGGGCGGTTGATGTCGGACTTTATTGCCGAGTATGCTCACCTGCCATTGATTCCGCCGTTTCTGCAACCGGGGTTTCGCCAGTATTCCGGCGGAGCGAATTTTGCATCTTCTGGAGCTGGTGCTCTGGTTGAGACGTTTCAAGGCTCGGTGATTGATCTGAAAACACAACTAAGAAATTACAAGAAGGTAGAGACCTGGTTCAGACACAAGTTGGGCGATGCTGAAGCCAAAATGACACTGTCAAGAGCCGTCTACTTGTTTAACATCGGAACCAACGATTACATGAGCCCTTTCTTGATCGACTCCCCCATTTTCAAGTCCCACTCTCACTCAGAATACGTTGGAATGGTCATTGGCAATTTGACAACCATCATCCAAGAGATTTATGCAGGAGGGGGTCGAAAATTTGGGTTCATCAACTTGTTGAGATTAGGTTGTCTTCCGGGCTTGAGAATAATCAAACAAGAAAACAATGGGAGCTGCTTAGAAGAAGTTTCATCACTTGCCAGATTACATAACCAAGCTCTCTCCAAAGTTCTCAGGAAACTAGGAAGTCAGTTGAAGGGTTTTAAATATTCACTCTACGACTTGAACCACAACTTTAGACAGAGAACCAGACATCCTTCTAAATATGGTTTTAAAGAAGGAAAATCAGCATGCTGCGGAACAGGTCAGTTTCGAGGGGTGTTCAGCTGTGGAGGGAAGAGAATCGTGAAGGAGTTTGAGTTGTGCGAAAATCCTAATGAATATCTGTTTTGGGACTCTTTCCATCTGACTGAAAAAGTTTATAGCCAATTGGCTTATCAAATGTGGAGTGGGGAAGATGACTCTAAGCCCTTGGGTCTATACAATCTGAAGGATCTCTTCAGAGATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

43.17

Weight (kDa)

8.97

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 355 1.1e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 309
AccI GTMKAC 2 cut(s) 21, 513
AciI CCGC 5 cut(s) 104, 265, 314, 351, 924
AclWI GGATC 1 cut(s) 1128
AcsI RAATTY 2 cut(s) 358, 665
AcuI CTGAAG 3 cut(s) 504, 1111, 1136
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 210, 264
AgsI TTSAA 7 cut(s) 60, 189, 235, 401, 577, 823, 853
AjnI CCWGG 2 cut(s) 198, 455
AloI GAACNNNNNNTCC 2 cut(s) 865, 897
AluBI AGCT 5 cut(s) 15, 377, 741, 785, 954
AluI AGCT 5 cut(s) 15, 377, 741, 785, 954
Alw21I GWGCWC 1 cut(s) 385
Alw26I GTCTC 4 cut(s) 138, 218, 386, 446
AlwI GGATC 1 cut(s) 1128
AoxI GGCC 2 cut(s) 201, 212
ApeKI GCWGC 2 cut(s) 741, 921
ApoI RAATTY 2 cut(s) 358, 665
AsuC2I CCSGG 2 cut(s) 330, 705
AsuHPI GGTGA 2 cut(s) 290, 421
BanII GRGCYC 1 cut(s) 551
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 728, 908
BccI CCATC 2 cut(s) 638, 1038
BceAI ACGGC 2 cut(s) 301, 494
BciT130I CCWGG 2 cut(s) 200, 457
BcnI CCSGG 2 cut(s) 330, 705
BcoDI GTCTC 4 cut(s) 138, 218, 386, 446
BfaI CTAG 1 cut(s) 809
BfuAI ACCTGC 1 cut(s) 309
BisI GCNGC 3 cut(s) 104, 742, 922
BlsI GCNGC 3 cut(s) 105, 743, 923
Bme1390I CCNGG 4 cut(s) 200, 330, 457, 705
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 4 cut(s) 200, 330, 457, 705
BmsI GCATC 4 cut(s) 115, 148, 374, 469
BpiI GAAGAC 1 cut(s) 692
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 730
BpuMI CCSGG 2 cut(s) 330, 705
BsaI GGTCTC 2 cut(s) 218, 446
BsaJI CCNNGG 2 cut(s) 329, 1098
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 264
Bse1I ACTGG 2 cut(s) 250, 340
BseBI CCWGG 2 cut(s) 200, 457
BseDI CCNNGG 2 cut(s) 329, 1098
BseGI GGATG 3 cut(s) 163, 633, 880
BseLI CCNNNNNNNGG 2 cut(s) 210, 264
BseMII CTCAG 2 cut(s) 606, 814
BseNI ACTGG 2 cut(s) 250, 340
BseXI GCAGC 2 cut(s) 728, 908
BseYI CCCAGC 1 cut(s) 87
BshFI GGCC 2 cut(s) 203, 214
BsiHKAI GWGCWC 1 cut(s) 385
BsiSI CCGG 3 cut(s) 329, 348, 704
BslFI GGGAC 2 cut(s) 565, 1034
BslI CCNNNNNNNGG 2 cut(s) 210, 264
BsmAI GTCTC 4 cut(s) 138, 218, 386, 446
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 565, 1034
BsmI GAATGC 1 cut(s) 91
BsnI GGCC 2 cut(s) 203, 214
Bso31I GGTCTC 2 cut(s) 218, 446
Bsp1286I GDGCHC 2 cut(s) 385, 551
Bsp143I GATC 3 cut(s) 415, 558, 1120
BspACI CCGC 5 cut(s) 104, 265, 314, 351, 924
BspANI GGCC 2 cut(s) 203, 214
BspCNI CTCAG 2 cut(s) 605, 813
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 532
BspMI ACCTGC 1 cut(s) 309
BspPI GGATC 1 cut(s) 1128
BspTNI GGTCTC 2 cut(s) 218, 446
BsrI ACTGG 2 cut(s) 250, 340
BssECI CCNNGG 2 cut(s) 329, 1098
BssMI GATC 3 cut(s) 415, 558, 1120
BssT1I CCWWGG 1 cut(s) 1098
Bst2UI CCWGG 2 cut(s) 200, 457
Bst4CI ACNGT 1 cut(s) 501
Bst6I CTCTTC 2 cut(s) 959, 1130
BstC8I GCNNGC 2 cut(s) 163, 919
BstDEI CTNAG 5 cut(s) 431, 592, 745, 800, 1092
BstF5I GGATG 3 cut(s) 163, 633, 880
BstKTI GATC 3 cut(s) 418, 561, 1123
BstMAI GTCTC 4 cut(s) 138, 218, 386, 446
BstMBI GATC 3 cut(s) 415, 558, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 293, 322, 362
BstNI CCWGG 2 cut(s) 200, 457
BstNSI RCATGY 1 cut(s) 921
BstSCI CCNGG 4 cut(s) 198, 328, 455, 703
BstV1I GCAGC 2 cut(s) 728, 908
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 1 cut(s) 1120
BstYI RGATCY 1 cut(s) 1120
BsuRI GGCC 2 cut(s) 203, 214
BtgZI GCGATG 1 cut(s) 492
BtsCI GGATG 3 cut(s) 163, 633, 880
BtsIMutI CAGTG 1 cut(s) 497
BveI ACCTGC 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 163, 919
CciI TCATGA 1 cut(s) 118
CsiI ACCWGGT 1 cut(s) 455
Csp6I GTAC 1 cut(s) 44
CviAII CATG 4 cut(s) 41, 119, 544, 918
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 5 cut(s) 431, 592, 745, 800, 1092
DpnI GATC 3 cut(s) 417, 560, 1122
DpnII GATC 3 cut(s) 415, 558, 1120
DraI TTTAAA 2 cut(s) 832, 901
Eam1104I CTCTTC 2 cut(s) 959, 1130
EarI CTCTTC 2 cut(s) 959, 1130
EciI GGCGGA 2 cut(s) 303, 366
Eco130I CCWWGG 1 cut(s) 1098
Eco147I AGGCCT 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 551
Eco31I GGTCTC 2 cut(s) 218, 446
Eco57I CTGAAG 3 cut(s) 504, 1111, 1136
EcoRII CCWGG 2 cut(s) 198, 455
EcoT14I CCWWGG 1 cut(s) 1098
EcoT38I GRGCYC 1 cut(s) 551
ErhI CCWWGG 1 cut(s) 1098
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 4 cut(s) 44, 122, 547, 921
FalI AAGNNNNNCTT 1 cut(s) 1109
FaqI GGGAC 2 cut(s) 565, 1034
FatI CATG 4 cut(s) 40, 118, 543, 917
FblI GTMKAC 2 cut(s) 21, 513
Fnu4HI GCNGC 3 cut(s) 104, 742, 922
FokI GGATG 3 cut(s) 170, 620, 867
FriOI GRGCYC 1 cut(s) 551
Fsp4HI GCNGC 3 cut(s) 104, 742, 922
FspBI CTAG 1 cut(s) 809
GluI GCNGC 3 cut(s) 104, 742, 922
GsaI CCCAGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 2 cut(s) 203, 214
HapII CCGG 3 cut(s) 329, 348, 704
Hin1II CATG 4 cut(s) 44, 122, 547, 921
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 7 cut(s) 146, 252, 310, 563, 969, 1022, 1088
HpaII CCGG 3 cut(s) 329, 348, 704
HphI GGTGA 2 cut(s) 290, 421
Hpy166II GTNNAC 2 cut(s) 22, 514
Hpy188III TCNNGA 7 cut(s) 119, 194, 372, 504, 556, 802, 973
Hpy8I GTNNAC 2 cut(s) 22, 514
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 6 cut(s) 439, 817, 894, 899, 970, 1111
HpyCH4III ACNGT 1 cut(s) 501
HpyCH4IV ACGT 2 cut(s) 395, 600
HpyCH4V TGCA 4 cut(s) 161, 325, 365, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 322, 362
HpyF3I CTNAG 5 cut(s) 431, 592, 745, 800, 1092
HpySE526I ACGT 2 cut(s) 395, 600
Hsp92II CATG 4 cut(s) 44, 122, 547, 921
Kzo9I GATC 3 cut(s) 415, 558, 1120
LmnI GCTCC 3 cut(s) 353, 374, 738
Lsp1109I GCAGC 2 cut(s) 728, 908
LweI GCATC 4 cut(s) 115, 148, 374, 469
MabI ACCWGGT 1 cut(s) 455
MaeI CTAG 1 cut(s) 809
MaeII ACGT 2 cut(s) 395, 600
MalI GATC 3 cut(s) 417, 560, 1122
MboI GATC 3 cut(s) 415, 558, 1120
MfeI CAATTG 1 cut(s) 1055
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 2 cut(s) 385, 551
MluCI AATT 6 cut(s) 205, 358, 436, 619, 665, 1055
MlyI GAGTC 4 cut(s) 140, 557, 1016, 1082
MmeI TCCRAC 3 cut(s) 255, 282, 583
MnlI CCTC 4 cut(s) 35, 647, 935, 953
MseI TTAA 4 cut(s) 77, 522, 831, 900
MslI CAYNNNNRTG 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 954
MspI CCGG 3 cut(s) 329, 348, 704
MspR9I CCNGG 4 cut(s) 200, 330, 457, 705
MunI CAATTG 1 cut(s) 1055
Mva1269I GAATGC 1 cut(s) 91
MvaI CCWGG 2 cut(s) 200, 457
MwoI GCNNNNNNNGC 3 cut(s) 293, 322, 362
NciI CCSGG 2 cut(s) 330, 705
NdeII GATC 3 cut(s) 415, 558, 1120
NlaIII CATG 4 cut(s) 44, 122, 547, 921
NlaIV GGNNCC 1 cut(s) 532
NmeAIII GCCGAG 1 cut(s) 313
NspI RCATGY 1 cut(s) 921
PaeI GCATGC 1 cut(s) 921
PagI TCATGA 1 cut(s) 118
PaqCI CACCTGC 1 cut(s) 309
PceI AGGCCT 1 cut(s) 214
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PctI GAATGC 1 cut(s) 91
PfeI GAWTC 3 cut(s) 252, 310, 969
PflFI GACNNNGTC 1 cut(s) 499
PkrI GCNGC 3 cut(s) 105, 743, 923
PleI GAGTC 4 cut(s) 140, 557, 1016, 1082
PpsI GAGTC 4 cut(s) 140, 557, 1016, 1082
Psp6I CCWGG 2 cut(s) 198, 455
PspFI CCCAGC 1 cut(s) 87
PspGI CCWGG 2 cut(s) 198, 455
PspN4I GGNNCC 1 cut(s) 532
PsuI RGATCY 1 cut(s) 1120
PsyI GACNNNGTC 1 cut(s) 499
PvuII CAGCTG 1 cut(s) 954
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 4 cut(s) 77, 522, 831, 900
SatI GCNGC 3 cut(s) 104, 742, 922
Sau3AI GATC 3 cut(s) 415, 558, 1120
SchI GAGTC 4 cut(s) 140, 557, 1016, 1082
ScrFI CCNGG 4 cut(s) 200, 330, 457, 705
SduI GDGCHC 2 cut(s) 385, 551
SexAI ACCWGGT 1 cut(s) 455
SfaNI GCATC 4 cut(s) 115, 148, 374, 469
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
SphI GCATGC 1 cut(s) 921
Sse9I AATT 6 cut(s) 205, 358, 436, 619, 665, 1055
SseBI AGGCCT 1 cut(s) 214
SsiI CCGC 5 cut(s) 104, 265, 314, 351, 924
SspI AATATT 2 cut(s) 54, 836
SspMI CTAG 1 cut(s) 809
StuI AGGCCT 1 cut(s) 214
StyD4I CCNGG 4 cut(s) 198, 328, 455, 703
StyI CCWWGG 1 cut(s) 1098
TaaI ACNGT 1 cut(s) 501
TaiI ACGT 2 cut(s) 398, 603
TaqI TCGA 3 cut(s) 561, 661, 940
TasI AATT 6 cut(s) 205, 358, 436, 619, 665, 1055
TatI WGTACW 1 cut(s) 43
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 3 cut(s) 252, 310, 969
Tru1I TTAA 4 cut(s) 77, 522, 831, 900
Tru9I TTAA 4 cut(s) 77, 522, 831, 900
TscAI CASTG 1 cut(s) 504
TseI GCWGC 2 cut(s) 741, 921
TspDTI ATGAA 6 cut(s) 29, 124, 135, 664, 747, 1020
TspRI CASTG 1 cut(s) 504
Tth111I GACNNNGTC 1 cut(s) 499
XapI RAATTY 2 cut(s) 358, 665
XceI RCATGY 1 cut(s) 921
XmiI GTMKAC 2 cut(s) 21, 513
XspI CTAG 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.