AT1G56630

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005988
Physical Location & Seq
Reverse (-)
21223406 .. 21227112
3707 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56630.1

Sequence Viewer

Length: 1485 bp
ATGGTTCAACCCAAAAATTATTTTGTTCTTGATCCGAGAGAAGCCACGGTTACGGATCTTATGCGTCTTCTCTTCTACTCGGATCTCGAGAATAGAAAGTTCGTTGATACTTCGGTAGAGAATCTTGAGAATCGTCTTTGCGAATTCAGGGGACGGTGGATCATTTTTGTATCCATCGTTGTTCAGAAACTCTTGATTATCCTCAGGAAACCTCTCTCGTTCTTGGGATTCGCTCTTGGCTTTTGGCTCAACCTTCCTTCTTCCAATGGTGGCTTCTTCAAGATATTCCTAAATCTTGTCAAAGGAAGGTTTATTTGGCCGGAAAAAACATCAGCGACATTTGCATCCATAAACGGAAACCTAGACCAAAAAGTAGAGTTAGGTTTAGGCCGGAGCATAAAGATAGGAGATGAAAGATACAAACCACTGTTGTCAATTATGGCTTCTAAGCTGGCTTACGAGAACGAACACTTTATCAGATCCGTTCTTCAAGACCATTGGCAGATGGACTTATTGGGTTTCTACAGCTGTCCGAATGATTTTGATCAAACGAGGTCAACAGAGGTGATTGTTATAAGGGACACAAAAGACAATCCGAACCTTATCGTCGTCAGTTTTAGAGGAACCGATCCTTTCAACGCCGATGATTGGTGTACAGACCTTGATCTCTCTTGGTACGAGATTGTTATGAATAAAAATAGGCACAACGTTATGAATGTGGGAAAGATCCACGGCGGATTCATGAAAGCTTTAGGCCTTCCGAAAGAAGGATGGTATGAAGAAATAAACTCTGACCAAACCCAAAATAAAACAAGTCAACTTGCATACTACACAATTTTACGTCAGTTAAAAGAGGTTTTCGAACAAAACCCAACATCCAAGTTTATCCTGTCCGGACATAGCCTAGGTGGAGCCCTAGCGATCCTGTTCACAGCCGTGTTAATTATGCATGATGAGAAGGAAATGTTGGAGAGACTTGAAGGAGTTTACACATTTGGGCAGCCACGCGTAGGAGACGAGGATTTCGGTATTTATATGAAAGACAAGTTAAAGGAGTTTGACGTGAAGTACAAAAGATATGTTTATTGCAACGACATGGTTCCTAGGTTGCCTTTTGACGACAAGACACTCATGTTCAAACATTTTGGGGGCTGCCTCTACTGTGACAGTTTCTACAAAGGAAAGGTAGAAGAAGAGGAGCCAAACAAAAACTACTTCAATATCTTTTGGGTTATACCGAAGATAATAAACGCGGTGTGGGAGTTGATAAGGAGTTTCATCATATCTTGTTGGCAAGGTAGAGAATACAGAGAAGGATGGTTATTGACGTGTTTTAGACTTGTCGCATTGTTGATTCCGGGACTTCCTGCTCATTTTCCTAATGAGTACGTCAATGTTGCTCTTTTGGGAAACTTTCCTCCTCAGGTACCTGATTCTCAAAATTTACCTGATGATGACCTCAATGTTGCCCTCTTGAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

494

Amino Acids

57.39

Weight (kDa)

5.87

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 203 - 373 1.2e-34 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
Acc65I GGTACC 1 cut(s) 1426
AccB1I GGYRCC 1 cut(s) 1426
AccII CGCG 2 cut(s) 1008, 1253
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 2 cut(s) 735, 1253
AclI AACGTT 1 cut(s) 708
AclWI GGATC 8 cut(s) 26, 63, 90, 167, 474, 623, 721, 916
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 2 cut(s) 143, 1441
AfaI GTAC 5 cut(s) 655, 677, 1070, 1388, 1428
AfiI CCNNNNNNNGG 3 cut(s) 648, 767, 1010
AflIII ACRYGT 2 cut(s) 1006, 1328
AgsI TTSAA 7 cut(s) 8, 280, 491, 637, 980, 1138, 1219
AjiI CACGTC 2 cut(s) 1063, 1329
AjuI GAANNNNNNNTTGG 6 cut(s) 257, 289, 298, 330, 950, 982
AleI CACNNNNGTG 1 cut(s) 935
AluBI AGCT 3 cut(s) 451, 528, 749
AluI AGCT 3 cut(s) 451, 528, 749
Alw26I GTCTC 2 cut(s) 967, 1008
AlwI GGATC 8 cut(s) 26, 63, 90, 167, 474, 623, 721, 916
Ama87I CYCGRG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 893
AoxI GGCC 3 cut(s) 317, 388, 754
ApeKI GCWGC 2 cut(s) 1000, 1152
ApoI RAATTY 2 cut(s) 143, 1441
ArsI GACNNNNNNTTYG 2 cut(s) 1007, 1039
Asp718I GGTACC 1 cut(s) 1426
AspA2I CCTAGG 2 cut(s) 904, 1103
AsuC2I CCSGG 1 cut(s) 1359
AsuHPI GGTGA 1 cut(s) 577
AsuII TTCGAA 1 cut(s) 861
AvaI CYCGRG 1 cut(s) 86
AvrII CCTAGG 2 cut(s) 904, 1103
AxyI CCTNAGG 2 cut(s) 203, 1422
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BanI GGYRCC 1 cut(s) 1426
BanII GRGCYC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 1012, 1139
BccI CCATC 4 cut(s) 182, 499, 765, 1311
BceAI ACGGC 2 cut(s) 748, 920
BciVI GTATCC 1 cut(s) 181
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 1359
BcoDI GTCTC 2 cut(s) 967, 1008
BfaI CTAG 4 cut(s) 362, 905, 917, 1104
BfmI CTRYAG 1 cut(s) 523
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 2 cut(s) 1001, 1153
BlnI CCTAGG 2 cut(s) 904, 1103
BlsI GCNGC 2 cut(s) 1002, 1154
Bme1390I CCNGG 1 cut(s) 1359
BmeT110I CYCGRG 1 cut(s) 86
BmgBI CACGTC 2 cut(s) 1063, 1329
BmiI GGNNCC 5 cut(s) 625, 913, 1101, 1200, 1428
BmrFI CCNGG 1 cut(s) 1359
BmsI GCATC 1 cut(s) 353
BpiI GAAGAC 1 cut(s) 59
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 1359
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 4 cut(s) 45, 730, 904, 1103
BsaWI WCCGGW 1 cut(s) 893
Bsc4I CCNNNNNNNGG 3 cut(s) 648, 767, 1010
Bse21I CCTNAGG 2 cut(s) 203, 1422
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 893
BseDI CCNNGG 4 cut(s) 45, 730, 904, 1103
BseGI GGATG 4 cut(s) 344, 776, 875, 1322
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 648, 767, 1010
BseMII CTCAG 2 cut(s) 217, 1436
BseRI GAGGAG 2 cut(s) 1211, 1410
BseXI GCAGC 2 cut(s) 1012, 1139
Bsh1236I CGCG 2 cut(s) 1008, 1253
BshFI GGCC 3 cut(s) 319, 390, 756
BshNI GGYRCC 1 cut(s) 1426
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 4 cut(s) 320, 391, 894, 1358
BslFI GGGAC 3 cut(s) 165, 593, 1374
BslI CCNNNNNNNGG 3 cut(s) 648, 767, 1010
BsmAI GTCTC 2 cut(s) 967, 1008
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 3 cut(s) 165, 593, 1374
BsnI GGCC 3 cut(s) 319, 390, 756
BsoBI CYCGRG 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 916
Bsp13I TCCGGA 1 cut(s) 893
Bsp1407I TGTACA 1 cut(s) 653
BspACI CCGC 2 cut(s) 735, 1253
BspANI GGCC 3 cut(s) 319, 390, 756
BspCNI CTCAG 2 cut(s) 216, 1435
BspEI TCCGGA 1 cut(s) 893
BspFNI CGCG 2 cut(s) 1008, 1253
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 5 cut(s) 625, 913, 1101, 1200, 1428
BspPI GGATC 8 cut(s) 26, 63, 90, 167, 474, 623, 721, 916
BspT104I TTCGAA 1 cut(s) 861
BspT107I GGYRCC 1 cut(s) 1426
BsrGI TGTACA 1 cut(s) 653
BssECI CCNNGG 4 cut(s) 45, 730, 904, 1103
BssT1I CCWWGG 2 cut(s) 904, 1103
Bst4CI ACNGT 5 cut(s) 49, 156, 429, 1163, 1169
Bst6I CTCTTC 2 cut(s) 77, 1188
BstAUI TGTACA 1 cut(s) 653
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 3 cut(s) 203, 447, 1422
BstDSI CCRYGG 2 cut(s) 45, 730
BstF5I GGATG 4 cut(s) 344, 776, 875, 1322
BstFNI CGCG 2 cut(s) 1008, 1253
BstMAI GTCTC 2 cut(s) 967, 1008
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstSCI CCNGG 1 cut(s) 1357
BstSFI CTRYAG 1 cut(s) 523
BstUI CGCG 2 cut(s) 1008, 1253
BstV1I GCAGC 2 cut(s) 1012, 1139
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 4 cut(s) 55, 82, 479, 726
BstYI RGATCY 4 cut(s) 55, 82, 479, 726
Bsu36I CCTNAGG 2 cut(s) 203, 1422
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 3 cut(s) 319, 390, 756
BtgI CCRYGG 2 cut(s) 45, 730
BtrI CACGTC 2 cut(s) 1063, 1329
BtsCI GGATG 4 cut(s) 344, 776, 875, 1322
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 453
CciI TCATGA 1 cut(s) 741
CseI GACGC 1 cut(s) 53
Csp6I GTAC 5 cut(s) 654, 676, 1069, 1387, 1427
CviAII CATG 4 cut(s) 742, 950, 1096, 1132
CviQI GTAC 5 cut(s) 654, 676, 1069, 1387, 1427
DdeI CTNAG 3 cut(s) 203, 447, 1422
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 2 cut(s) 77, 1188
EarI CTCTTC 2 cut(s) 77, 1188
EciI GGCGGA 1 cut(s) 750
Eco130I CCWWGG 2 cut(s) 904, 1103
Eco147I AGGCCT 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 916
Eco81I CCTNAGG 2 cut(s) 203, 1422
Eco88I CYCGRG 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 904, 1103
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 1 cut(s) 916
ErhI CCWWGG 2 cut(s) 904, 1103
Esp3I CGTCTC 1 cut(s) 1008
FaeI CATG 4 cut(s) 745, 953, 1099, 1135
FaqI GGGAC 3 cut(s) 165, 593, 1374
FatI CATG 4 cut(s) 741, 949, 1095, 1131
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 1001, 1153
FokI GGATG 4 cut(s) 331, 783, 862, 1329
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 2 cut(s) 1001, 1153
FspBI CTAG 4 cut(s) 362, 905, 917, 1104
GluI GCNGC 2 cut(s) 1001, 1153
HaeIII GGCC 3 cut(s) 319, 390, 756
HapII CCGG 4 cut(s) 320, 391, 894, 1358
HgaI GACGC 1 cut(s) 53
Hin1II CATG 4 cut(s) 745, 953, 1099, 1135
HincII GTYRAC 2 cut(s) 558, 818
HindII GTYRAC 2 cut(s) 558, 818
HindIII AAGCTT 1 cut(s) 747
HinfI GANTC 6 cut(s) 121, 130, 228, 738, 1354, 1433
HpaII CCGG 4 cut(s) 320, 391, 894, 1358
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 5 cut(s) 558, 654, 818, 930, 988
Hpy188I TCNGA 8 cut(s) 36, 82, 186, 479, 534, 597, 762, 793
Hpy8I GTNNAC 5 cut(s) 558, 654, 818, 930, 988
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 8 cut(s) 263, 267, 300, 761, 767, 952, 974, 1307
HpyCH4III ACNGT 5 cut(s) 49, 156, 429, 1163, 1169
HpyCH4IV ACGT 5 cut(s) 708, 841, 1062, 1328, 1389
HpyCH4V TGCA 4 cut(s) 344, 824, 949, 1089
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 3 cut(s) 203, 447, 1422
HpySE526I ACGT 5 cut(s) 708, 841, 1062, 1328, 1389
Hsp92II CATG 4 cut(s) 745, 953, 1099, 1135
Kpn2I TCCGGA 1 cut(s) 893
KpnI GGTACC 1 cut(s) 1430
Ksp22I TGATCA 1 cut(s) 544
LmnI GCTCC 3 cut(s) 393, 911, 1198
Lsp1109I GCAGC 2 cut(s) 1012, 1139
LweI GCATC 1 cut(s) 353
MaeI CTAG 4 cut(s) 362, 905, 917, 1104
MaeII ACGT 5 cut(s) 708, 841, 1062, 1328, 1389
MaeIII GTNAC 2 cut(s) 49, 1163
MboII GAAGA 9 cut(s) 59, 64, 252, 268, 479, 791, 1202, 1205, 1252
MflI RGATCY 4 cut(s) 55, 82, 479, 726
MhlI GDGCHC 1 cut(s) 916
MluCI AATT 6 cut(s) 16, 143, 435, 834, 942, 1441
MluI ACGCGT 1 cut(s) 1006
MmeI TCCRAC 1 cut(s) 948
Mph1103I ATGCAT 1 cut(s) 951
MroI TCCGGA 1 cut(s) 893
MseI TTAA 3 cut(s) 848, 941, 1049
MslI CAYNNNNRTG 1 cut(s) 935
MspA1I CMGCKG 1 cut(s) 528
MspI CCGG 4 cut(s) 320, 391, 894, 1358
MspR9I CCNGG 1 cut(s) 1359
MvnI CGCG 2 cut(s) 1008, 1253
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 1 cut(s) 1359
NlaIII CATG 4 cut(s) 745, 953, 1099, 1135
NlaIV GGNNCC 5 cut(s) 625, 913, 1101, 1200, 1428
NmuCI GTSAC 1 cut(s) 1163
NsiI ATGCAT 1 cut(s) 951
NspV TTCGAA 1 cut(s) 861
OliI CACNNNNGTG 1 cut(s) 935
PaeR7I CTCGAG 1 cut(s) 86
PagI TCATGA 1 cut(s) 741
PceI AGGCCT 1 cut(s) 756
PfeI GAWTC 6 cut(s) 121, 130, 228, 738, 1354, 1433
PfoI TCCNGGA 1 cut(s) 1357
PkrI GCNGC 2 cut(s) 1002, 1154
PsiI TTATAA 1 cut(s) 575
Psp1406I AACGTT 1 cut(s) 708
PspN4I GGNNCC 5 cut(s) 625, 913, 1101, 1200, 1428
PsuI RGATCY 4 cut(s) 55, 82, 479, 726
PvuII CAGCTG 1 cut(s) 528
RsaI GTAC 5 cut(s) 655, 677, 1070, 1388, 1428
RsaNI GTAC 5 cut(s) 654, 676, 1069, 1387, 1427
RseI CAYNNNNRTG 1 cut(s) 935
SaqAI TTAA 3 cut(s) 848, 941, 1049
SatI GCNGC 2 cut(s) 1001, 1153
ScrFI CCNGG 1 cut(s) 1359
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 1 cut(s) 353
SfcI CTRYAG 1 cut(s) 523
Sfr274I CTCGAG 1 cut(s) 86
SfuI TTCGAA 1 cut(s) 861
SlaI CTCGAG 1 cut(s) 86
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 3 cut(s) 86, 125, 1474
SmoI CTYRAG 3 cut(s) 86, 125, 1474
Sse9I AATT 6 cut(s) 16, 143, 435, 834, 942, 1441
SseBI AGGCCT 1 cut(s) 756
SsiI CCGC 2 cut(s) 735, 1253
SspMI CTAG 4 cut(s) 362, 905, 917, 1104
StuI AGGCCT 1 cut(s) 756
StyD4I CCNGG 1 cut(s) 1357
StyI CCWWGG 2 cut(s) 904, 1103
TaaI ACNGT 5 cut(s) 49, 156, 429, 1163, 1169
TaiI ACGT 5 cut(s) 711, 844, 1065, 1331, 1392
TaqI TCGA 2 cut(s) 87, 861
TasI AATT 6 cut(s) 16, 143, 435, 834, 942, 1441
TatI WGTACW 2 cut(s) 653, 1068
TfiI GAWTC 6 cut(s) 121, 130, 228, 738, 1354, 1433
Tru1I TTAA 3 cut(s) 848, 941, 1049
Tru9I TTAA 3 cut(s) 848, 941, 1049
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 1163
TseI GCWGC 2 cut(s) 1000, 1152
Tsp45I GTSAC 1 cut(s) 1163
TspDTI ATGAA 8 cut(s) 426, 704, 728, 730, 758, 792, 1052, 1267
TspGWI ACGGA 3 cut(s) 68, 369, 472
TspRI CASTG 1 cut(s) 432
XapI RAATTY 2 cut(s) 143, 1441
XhoI CTCGAG 1 cut(s) 86
XmaJI CCTAGG 2 cut(s) 904, 1103
XspI CTAG 4 cut(s) 362, 905, 917, 1104
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.