Rroxscaffold_7G00160590

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005988
Physical Location & Seq
Forward (+)
3549728 .. 3562032
12305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160590.1

Sequence Viewer

Length: 888 bp
ATGGATCTCATGTTCCTGCAAGTGTCACCATTTTCTGGTGATCTCCACGGCTCCGCCGTGTTCTTCGACACCGGAGACATTTCGGGCACTTCGGGCTTAGTTTCCATCTCGAGTTCACCTGTAGACGAGGAATTAAGAGGCTTGTTGGGTTGTTGGCATCAAAATCTTCAGCTCGCAATGTTCCAGTTCCGGCAAGAGACATTTGACATAGCTAGCTCATGGATCGATCTCCTTGCTTCCAAGCCATTCCAACCCAATATTAACATGGGTTCTAAGGAACATGTATGTGACAATTACGTGTTTTTGAAGCCAAAAGAAGTAACTCTCATCGAGCTTATTCGCTTTCTGTTTTCCTCCGATTTAGAGAACAGAAGATTCATAAATTCCTCGGAAGAGGAAGGGAAGAAGGGAGCGAATTTAGAACGGAGGTGGATAATCTTCATCTCTGTTGTAGTGCAATGGGTTTTCCTCCTTTGCTGTGTCCCTATGGCGAGAATAGGGGATGTGCTCCAGAAATGGCTCAACCTTGTCTCGACCAACGGCGGACCAGTCATGCTCTTCTTAAACTGGCTAAGAGGAGAGGTGGTCGAGCCGGATTCATTATTATCGACATCAGTGTTGGGGAATCTTGACAGGAGAATTGAATTAGACAAGAGCATCAAACCTGAGGATACAAGATACAAAGCGGCTCTCTCCATCATGGCCGCTAAGCAGTCATACGAGAACAAAGATCTCGTTCAATCCGTCGCTAATCATCACTGGAAGCGAAGAAACTTGAGACGGTTTTGTTTGGCGGCAATGGGATCGGCGAGCGCAGCAGCTTTCGAGAAGCTAGACCGGTTCGCCGGAGAACTGAGCCGGAACATCGAGGAGGAGTTGCGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

295

Amino Acids

33.48

Weight (kDa)

5.68

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 35
AccI GTMKAC 1 cut(s) 123
AciI CCGC 5 cut(s) 54, 543, 686, 705, 794
AclWI GGATC 3 cut(s) 12, 230, 811
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 2 cut(s) 382, 415
AcuI CTGAAG 1 cut(s) 152
AfiI CCNNNNNNNGG 1 cut(s) 35
AflIII ACRYGT 2 cut(s) 280, 297
AgeI ACCGGT 1 cut(s) 837
AgsI TTSAA 3 cut(s) 307, 644, 740
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 172, 212, 216, 334, 821, 832
AluI AGCT 6 cut(s) 172, 212, 216, 334, 821, 832
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 4 cut(s) 69, 191, 535, 772
AlwI GGATC 3 cut(s) 12, 230, 811
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 2 cut(s) 815, 818
ApoI RAATTY 2 cut(s) 382, 415
AsiGI ACCGGT 1 cut(s) 837
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 1 cut(s) 545
AsuHPI GGTGA 3 cut(s) 18, 50, 108
AsuNHI GCTAGC 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 545
AxyI CCTNAGG 1 cut(s) 666
BaeGI GKGCMC 1 cut(s) 89
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 827, 830
BccI CCATC 2 cut(s) 113, 704
BceAI ACGGC 3 cut(s) 41, 64, 556
BcgI CGANNNNNNTGC 4 cut(s) 215, 249, 786, 820
BciVI GTATCC 1 cut(s) 664
BcoDI GTCTC 4 cut(s) 69, 191, 535, 772
BfaI CTAG 2 cut(s) 213, 833
BfmI CTRYAG 1 cut(s) 120
BfuI GTATCC 1 cut(s) 664
BglII AGATCT 1 cut(s) 730
BisI GCNGC 5 cut(s) 687, 705, 795, 816, 819
BlpI GCTNAGC 1 cut(s) 708
BlsI GCNGC 5 cut(s) 688, 706, 796, 817, 820
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 52
BmsI GCATC 2 cut(s) 166, 666
BmtI GCTAGC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 494
Bpu1102I GCTNAGC 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 796
Bsa29I ATCGAT 1 cut(s) 225
BsaAI YACGTR 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 46, 387
BsaWI WCCGGW 2 cut(s) 71, 837
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse118I RCCGGY 1 cut(s) 837
Bse1I ACTGG 4 cut(s) 184, 548, 572, 764
Bse21I CCTNAGG 1 cut(s) 666
Bse3DI GCAATG 3 cut(s) 183, 464, 804
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 2 cut(s) 46, 387
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 3 cut(s) 183, 464, 804
BseMII CTCAG 2 cut(s) 657, 845
BseNI ACTGG 4 cut(s) 184, 548, 572, 764
BseRI GAGGAG 3 cut(s) 591, 884, 887
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 2 cut(s) 827, 830
BshFI GGCC 1 cut(s) 704
BshTI ACCGGT 1 cut(s) 837
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 6 cut(s) 72, 190, 593, 838, 846, 859
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 4 cut(s) 69, 191, 535, 772
BsmBI CGTCTC 1 cut(s) 772
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 1 cut(s) 704
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 89, 510
Bsp143I GATC 6 cut(s) 4, 40, 222, 226, 730, 803
Bsp1720I GCTNAGC 1 cut(s) 708
BspACI CCGC 5 cut(s) 54, 543, 686, 705, 794
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 2 cut(s) 658, 846
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 52
BspOI GCTAGC 1 cut(s) 216
BspPI GGATC 3 cut(s) 12, 230, 811
BspQI GCTCTTC 1 cut(s) 563
BsrDI GCAATG 3 cut(s) 183, 464, 804
BsrFI RCCGGY 1 cut(s) 837
BsrI ACTGG 4 cut(s) 184, 548, 572, 764
BssAI RCCGGY 1 cut(s) 837
BssECI CCNNGG 2 cut(s) 46, 387
BssMI GATC 6 cut(s) 4, 40, 222, 226, 730, 803
Bst4CI ACNGT 1 cut(s) 783
Bst6I CTCTTC 2 cut(s) 387, 563
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 3 cut(s) 174, 214, 811
BstDEI CTNAG 6 cut(s) 97, 273, 572, 666, 708, 854
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 508
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 6 cut(s) 7, 43, 225, 229, 733, 806
BstMAI GTCTC 4 cut(s) 69, 191, 535, 772
BstMBI GATC 6 cut(s) 4, 40, 222, 226, 730, 803
BstMWI GCNNNNNNNGC 2 cut(s) 93, 815
BstNSI RCATGY 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 120
BstSLI GKGCMC 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 827, 830
BstX2I RGATCY 2 cut(s) 4, 730
BstYI RGATCY 2 cut(s) 4, 730
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 666
BsuI GTATCC 1 cut(s) 664
BsuRI GGCC 1 cut(s) 704
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 621, 757
Cac8I GCNNGC 3 cut(s) 174, 214, 811
CfoI GCGC 1 cut(s) 815
Cfr10I RCCGGY 1 cut(s) 837
Cfr13I GGNCC 1 cut(s) 545
ClaI ATCGAT 1 cut(s) 225
CspAI ACCGGT 1 cut(s) 837
CviAII CATG 6 cut(s) 10, 219, 265, 281, 553, 700
DdeI CTNAG 6 cut(s) 97, 273, 572, 666, 708, 854
DpnI GATC 6 cut(s) 6, 42, 224, 228, 732, 805
DpnII GATC 6 cut(s) 4, 40, 222, 226, 730, 803
EaeI YGGCCR 1 cut(s) 702
Eam1104I CTCTTC 2 cut(s) 387, 563
EarI CTCTTC 2 cut(s) 387, 563
EciI GGCGGA 2 cut(s) 43, 558
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 152
Eco81I CCTNAGG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 6 cut(s) 13, 222, 268, 284, 556, 703
FaqI GGGAC 1 cut(s) 467
FatI CATG 6 cut(s) 9, 218, 264, 280, 552, 699
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 687, 705, 795, 816, 819
FokI GGATG 1 cut(s) 515
Fsp4HI GCNGC 5 cut(s) 687, 705, 795, 816, 819
FspBI CTAG 2 cut(s) 213, 833
GlaI GCGC 1 cut(s) 814
GluI GCNGC 5 cut(s) 687, 705, 795, 816, 819
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 1 cut(s) 704
HapII CCGG 6 cut(s) 72, 190, 593, 838, 846, 859
HhaI GCGC 1 cut(s) 815
Hin1II CATG 6 cut(s) 13, 222, 268, 284, 556, 703
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HinfI GANTC 3 cut(s) 375, 596, 625
HpaII CCGG 6 cut(s) 72, 190, 593, 838, 846, 859
HphI GGTGA 3 cut(s) 18, 50, 108
Hpy166II GTNNAC 2 cut(s) 116, 124
Hpy188I TCNGA 2 cut(s) 358, 391
Hpy188III TCNNGA 5 cut(s) 109, 511, 532, 629, 826
Hpy8I GTNNAC 2 cut(s) 116, 124
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 392, 400
HpyCH4III ACNGT 1 cut(s) 783
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 2 cut(s) 19, 457
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 815
HpyF3I CTNAG 6 cut(s) 97, 273, 572, 666, 708, 854
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 6 cut(s) 13, 222, 268, 284, 556, 703
HspAI GCGC 1 cut(s) 813
Kzo9I GATC 6 cut(s) 4, 40, 222, 226, 730, 803
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 3 cut(s) 56, 410, 513
Lsp1109I GCAGC 2 cut(s) 827, 830
LweI GCATC 2 cut(s) 166, 666
MaeI CTAG 2 cut(s) 213, 833
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 3 cut(s) 24, 287, 319
MalI GATC 6 cut(s) 6, 42, 224, 228, 732, 805
MboI GATC 6 cut(s) 4, 40, 222, 226, 730, 803
MboII GAAGA 8 cut(s) 55, 158, 384, 404, 415, 430, 550, 780
MflI RGATCY 2 cut(s) 4, 730
MhlI GDGCHC 2 cut(s) 89, 510
MluCI AATT 6 cut(s) 131, 292, 382, 415, 639, 644
MmeI TCCRAC 1 cut(s) 274
MseI TTAA 3 cut(s) 134, 261, 563
MslI CAYNNNNRTG 1 cut(s) 285
MspI CCGG 6 cut(s) 72, 190, 593, 838, 846, 859
MwoI GCNNNNNNNGC 2 cut(s) 93, 815
NdeII GATC 6 cut(s) 4, 40, 222, 226, 730, 803
NheI GCTAGC 1 cut(s) 212
NlaIII CATG 6 cut(s) 13, 222, 268, 284, 556, 703
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 24, 287
NspI RCATGY 1 cut(s) 284
PaeR7I CTCGAG 1 cut(s) 109
PciI ACATGT 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 563
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 741
PfeI GAWTC 3 cut(s) 375, 596, 625
PflMI CCANNNNNTGG 1 cut(s) 35
PinAI ACCGGT 1 cut(s) 837
PkrI GCNGC 5 cut(s) 688, 706, 796, 817, 820
Ppu21I YACGTR 1 cut(s) 298
PscI ACATGT 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 545
PsuI RGATCY 2 cut(s) 4, 730
RseI CAYNNNNRTG 1 cut(s) 285
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 3 cut(s) 134, 261, 563
SatI GCNGC 5 cut(s) 687, 705, 795, 816, 819
Sau3AI GATC 6 cut(s) 4, 40, 222, 226, 730, 803
Sau96I GGNCC 1 cut(s) 545
SduI GDGCHC 2 cut(s) 89, 510
SfaNI GCATC 2 cut(s) 166, 666
SfcI CTRYAG 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 109
SinI GGWCC 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 109, 775
SmoI CTYRAG 2 cut(s) 109, 775
Sse9I AATT 6 cut(s) 131, 292, 382, 415, 639, 644
SsiI CCGC 5 cut(s) 54, 543, 686, 705, 794
SspI AATATT 1 cut(s) 259
SspMI CTAG 2 cut(s) 213, 833
TaaI ACNGT 1 cut(s) 783
TaiI ACGT 1 cut(s) 300
TaqI TCGA 9 cut(s) 66, 110, 225, 330, 533, 588, 608, 825, 867
TasI AATT 6 cut(s) 131, 292, 382, 415, 639, 644
TauI GCSGC 3 cut(s) 689, 707, 797
TfiI GAWTC 3 cut(s) 375, 596, 625
Tru1I TTAA 3 cut(s) 134, 261, 563
Tru9I TTAA 3 cut(s) 134, 261, 563
TscAI CASTG 2 cut(s) 621, 764
TseFI GTSAC 2 cut(s) 24, 287
TseI GCWGC 2 cut(s) 815, 818
Tsp45I GTSAC 2 cut(s) 24, 287
TspDTI ATGAA 3 cut(s) 367, 430, 588
TspGWI ACGGA 2 cut(s) 439, 733
TspRI CASTG 2 cut(s) 621, 764
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 2 cut(s) 382, 415
XceI RCATGY 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 2 cut(s) 213, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.