Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0005988

Physical Location & Seq

Genomic Coordinates Forward (+)

4676331 .. 4679271

Gene Structure 2941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g07590.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1470 bp

ATGGCTTCTAAGGAACATGTCTGTCACAATTATGTTTTCCTCAAGCCAGAAGAAGCAAGCTTCATCGAGCTCATTCGCCTCCTGTTTTCCTCCGACGTAGAAAAGAGAAGCTTCATCAACTGCTCGGAAGAGGAAGGACGTAAACGACGAAAACTTGAACGCCGATGGCTAATCTTCGTCTCTATTGTGGTGCAATGGATGTTCCTTTTCTTCAGAACGCCCATGGCTCGAACTGGGGCTACCGTCAACAAGTTTCTAAATCTTGTAGCATCCAATGGCGGATTGTTCGCGCTCTTCAAAATGTGGCTTAAAGGAAAGGTGGCAAAGGCAGATTCGTTAACAACCAGATCACTGCTGGGAAATCTTGATAGGCGAGTGGAATTGGACGACAACATCAAACCAGGCAATACAAGATACAAAGGAGCTCTGTCCATGATGGCGGCTAAAATGTCATACGAAAACCAAGTCCTCATTAAAACAATCGTCACGGATCACTGGAAGATGAAATTCGTAGAGTTTTACAACTTTTGGAATGGTTATCAGAGACGAGATTCAACGCAAGCCTTCCTGTTCCAAGACACAACATCTGACCCCAACTTGATTGTGGTTGCATTTAGAGGCACCAACCCCTTTGATGCAGACGCATGGAGGACGGATTTCGACATCTCATGGTGCGAGTTTGCAGGACTCGGCAAGACCCACAGCGGCTTCATGAAAGCTCTTGGCATGCAACCAAACAGAAGCTGGCCCCTTGAACAAGAAACACAAGAAGTCAACGCCGCTGAACAACTTCCTCATCAGTTTGCTTACTACAAAATCAGACAAATTCTGATGGATTTAATGCAAGAAAACAAGAATGCCAAGTTCATACTGACAGGCCACAGCTTGGGCGGGGCATTGGCTGTGCTGTTTGCCGCGGTGCTTGCAATGCACGACTACGAGGAGAAATATGCATGGTTGATGGGGAGGTTGGAAGGGGTGTACACTTTTGGACAGCCAAGGGTGGGGGATGAGAACTTTGGGGTGTTTATGAGGGAGAAGCTGAAAAATTATGGTGTGAAGTATATGAGGTATGTTTATTCCAATGATATGGTTCCTAGGATACCTTATGACGATAAAACTCTCTTGTTTAAGCACTTTGGTCCAAGCCTATATTTCAGCAGCTGCTATAAGGGGAAGGTTTTGGAGGAGGAACCCAACAAGAACTACTTCAATGTGTTCTGGTTCATACCAAAGTGCCTAAATGCATTGTGGGAGCTGATTAGGAGCTTCATACTGCCGTTAATAAGGGGTGCGGAATACAAGGAAAGCTGGTTTATGAGGATGCTTAGGGTAGTTGGTTTAGTAATTCCTGGATTGGCAGCTCATGCTCCTCAAGATTATGTCAACATTACTCGATTGGGATCCCTTCCTTCCCTTGCTCACGTCCAAAAACGTATTCCTCTAGTAAATAATTCTAATGTCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005615 GO:0044421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

490

Amino Acids

56.78

Weight (kDa)

9.29

Isoelectric Point (pI)

40.31

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56630 AT5G42930 AT5G42930
fragaria_vesca	FvH4_2g34290
malus_domestica	MD15G1081200.v1.1 MD15G1081300.v1.1
prunus_persica	Prupe.1G434100_v2.0.a1 Prupe.1G434100_v2.0.a1 Prupe.1G434100_v2.0.a1
pyrus_communis	pycom15g07570 pycom15g07590 pycom15g07600
rosa_chinensis	RchiOBHm_Chr6g0308081
rosa_laevigata	RLG00000010669 RLG00000010670
rosa_multiflora	Rmu_sc0002624.1_g000005
rosa_roxburghii	Rroxscaffold_7G00160580 Rroxscaffold_7G00160590 Rroxscaffold_7G00160600
rosa_rugosa	Rorug06G0363600 Rorug06G0363900
rosa_samantha	Rh6AG472600 Rh6BG483700 Rh6CG488500 Rh6CG488600 Rh6DG475000
rosa_wichuraiana	Rw6G041240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	620
AccB7I	CCANNNNNTGG	1 cut(s)	886
AccII	CGCG	2 cut(s)	290, 917
AciI	CCGC	8 cut(s)	279, 440, 705, 780, 891, 915, 917, 1295
AclWI	GGATC	3 cut(s)	498, 1398, 1411
AcsI	RAATTY	2 cut(s)	506, 825
AcuI	CTGAAG	1 cut(s)	196
AfaI	GTAC	1 cut(s)	983
AfiI	CCNNNNNNNGG	2 cut(s)	886, 1004
AflIII	ACRYGT	1 cut(s)	16
AgsI	TTSAA	5 cut(s)	158, 298, 555, 755, 1213
AjiI	CACGTC	1 cut(s)	1426
AjnI	CCWGG	2 cut(s)	400, 1351
AjuI	GAANNNNNNNTTGG	2 cut(s)	1422, 1454
Alw21I	GWGCWC	2 cut(s)	72, 427
Alw26I	GTCTC	2 cut(s)	184, 538
AlwI	GGATC	3 cut(s)	498, 1398, 1411
AlwNI	CAGNNNCTG	2 cut(s)	744, 1164
AoxI	GGCC	2 cut(s)	746, 877
ApeKI	GCWGC	3 cut(s)	1161, 1164, 1361
ApoI	RAATTY	2 cut(s)	506, 825
ArsI	GACNNNNNNTTYG	2 cut(s)	450, 482
Asp700I	GAANNNNTTC	2 cut(s)	789, 1208
AspA2I	CCTAGG	1 cut(s)	1097
AspLEI	GCGC	1 cut(s)	292
AspS9I	GGNCC	2 cut(s)	747, 1142
AvaII	GGWCC	1 cut(s)	1142
AvrII	CCTAGG	1 cut(s)	1097
BamHI	GGATCC	1 cut(s)	1403
BanI	GGYRCC	1 cut(s)	620
BanII	GRGCYC	2 cut(s)	72, 427
Bbv12I	GWGCWC	2 cut(s)	72, 427
BbvI	GCAGC	3 cut(s)	1151, 1173, 1373
BccI	CCATC	4 cut(s)	159, 430, 826, 955
BceAI	ACGGC	1 cut(s)	1264
BciT130I	CCWGG	2 cut(s)	402, 1353
BciVI	GTATCC	1 cut(s)	1095
BcoDI	GTCTC	2 cut(s)	184, 538
BfaI	CTAG	2 cut(s)	1098, 1445
BfuI	GTATCC	1 cut(s)	1095
BisI	GCNGC	7 cut(s)	441, 706, 780, 915, 1162, 1165, 1362
BlnI	CCTAGG	1 cut(s)	1097
BlsI	GCNGC	7 cut(s)	442, 707, 781, 916, 1163, 1166, 1363
Bme1390I	CCNGG	2 cut(s)	402, 1353
Bme18I	GGWCC	1 cut(s)	1142
BmgBI	CACGTC	1 cut(s)	1426
BmgT120I	GGNCC	2 cut(s)	747, 1142
BmiI	GGNNCC	5 cut(s)	622, 749, 1095, 1194, 1405
BmrFI	CCNGG	2 cut(s)	402, 1353
BmrI	ACTGGG	1 cut(s)	243
BmsI	GCATC	3 cut(s)	278, 625, 1314
BmuI	ACTGGG	1 cut(s)	243
Bpu10I	CCTNAGC	1 cut(s)	1328
BpuEI	CTTGAG	2 cut(s)	26, 1359
BsaBI	GATNNNNATC	1 cut(s)	1402
BsaJI	CCNNGG	4 cut(s)	222, 915, 998, 1097
Bsc4I	CCNNNNNNNGG	2 cut(s)	886, 1004
Bse1I	ACTGG	2 cut(s)	238, 500
Bse3DI	GCAATG	2 cut(s)	200, 933
Bse8I	GATNNNNATC	1 cut(s)	1402
BseBI	CCWGG	2 cut(s)	402, 1353
BseDI	CCNNGG	4 cut(s)	222, 915, 998, 1097
BseGI	GGATG	4 cut(s)	204, 269, 1015, 1329
BseJI	GATNNNNATC	1 cut(s)	1402
BseLI	CCNNNNNNNGG	2 cut(s)	886, 1004
BseMI	GCAATG	2 cut(s)	200, 933
BseNI	ACTGG	2 cut(s)	238, 500
BseRI	GAGGAG	3 cut(s)	956, 1202, 1362
BseXI	GCAGC	3 cut(s)	1151, 1173, 1373
BseYI	CCCAGC	1 cut(s)	355
Bsh1236I	CGCG	2 cut(s)	290, 917
BshFI	GGCC	2 cut(s)	748, 879
BshNI	GGYRCC	1 cut(s)	620
BsiHKAI	GWGCWC	2 cut(s)	72, 427
BslI	CCNNNNNNNGG	2 cut(s)	886, 1004
BsmAI	GTCTC	2 cut(s)	184, 538
BsmBI	CGTCTC	2 cut(s)	184, 538
BsmI	GAATGC	1 cut(s)	862
BsnI	GGCC	2 cut(s)	748, 879
Bsp1286I	GDGCHC	2 cut(s)	72, 427
Bsp1407I	TGTACA	1 cut(s)	981
Bsp143I	GATC	3 cut(s)	347, 490, 1403
Bsp19I	CCATGG	1 cut(s)	222
BspACI	CCGC	8 cut(s)	279, 440, 705, 780, 891, 915, 917, 1295
BspANI	GGCC	2 cut(s)	748, 879
BspFNI	CGCG	2 cut(s)	290, 917
BspHI	TCATGA	1 cut(s)	711
BspLI	GGNNCC	5 cut(s)	622, 749, 1095, 1194, 1405
BspPI	GGATC	3 cut(s)	498, 1398, 1411
BspQI	GCTCTTC	1 cut(s)	299
BspT107I	GGYRCC	1 cut(s)	620
BsrDI	GCAATG	2 cut(s)	200, 933
BsrGI	TGTACA	1 cut(s)	981
BsrI	ACTGG	2 cut(s)	238, 500
BssECI	CCNNGG	4 cut(s)	222, 915, 998, 1097
BssMI	GATC	3 cut(s)	347, 490, 1403
BssT1I	CCWWGG	3 cut(s)	222, 998, 1097
Bst2UI	CCWGG	2 cut(s)	402, 1353
Bst4CI	ACNGT	1 cut(s)	244
Bst6I	CTCTTC	2 cut(s)	123, 299
BstAPI	GCANNNNNTGC	1 cut(s)	1367
BstAUI	TGTACA	1 cut(s)	981
BstC8I	GCNNGC	5 cut(s)	58, 561, 728, 746, 924
BstDEI	CTNAG	2 cut(s)	9, 1328
BstDSI	CCRYGG	2 cut(s)	222, 915
BstF5I	GGATG	4 cut(s)	204, 269, 1015, 1329
BstFNI	CGCG	2 cut(s)	290, 917
BstHHI	GCGC	1 cut(s)	292
BstKTI	GATC	3 cut(s)	350, 493, 1406
BstMAI	GTCTC	2 cut(s)	184, 538
BstMBI	GATC	3 cut(s)	347, 490, 1403
BstMWI	GCNNNNNNNGC	3 cut(s)	923, 928, 1367
BstNI	CCWGG	2 cut(s)	402, 1353
BstNSI	RCATGY	2 cut(s)	20, 730
BstSCI	CCNGG	2 cut(s)	400, 1351
BstUI	CGCG	2 cut(s)	290, 917
BstV1I	GCAGC	3 cut(s)	1151, 1173, 1373
BstX2I	RGATCY	1 cut(s)	1403
BstXI	CCANNNNNNTGG	1 cut(s)	1090
BstYI	RGATCY	1 cut(s)	1403
BsuI	GTATCC	1 cut(s)	1095
BsuRI	GGCC	2 cut(s)	748, 879
BtgI	CCRYGG	2 cut(s)	222, 915
BtrI	CACGTC	1 cut(s)	1426
BtsCI	GGATG	4 cut(s)	204, 269, 1015, 1329
BtsI	GCAGTG	1 cut(s)	350
BtsIMutI	CAGTG	2 cut(s)	350, 493
Cac8I	GCNNGC	5 cut(s)	58, 561, 728, 746, 924
CaiI	CAGNNNCTG	2 cut(s)	744, 1164
CciI	TCATGA	1 cut(s)	711
CfoI	GCGC	1 cut(s)	292
Cfr13I	GGNCC	2 cut(s)	747, 1142
Cfr42I	CCGCGG	1 cut(s)	918
CseI	GACGC	1 cut(s)	650
Csp6I	GTAC	1 cut(s)	982
CviAII	CATG	9 cut(s)	17, 223, 433, 645, 669, 712, 727, 954, 1367
CviQI	GTAC	1 cut(s)	982
DdeI	CTNAG	2 cut(s)	9, 1328
DpnI	GATC	3 cut(s)	349, 492, 1405
DpnII	GATC	3 cut(s)	347, 490, 1403
Eam1104I	CTCTTC	2 cut(s)	123, 299
EarI	CTCTTC	2 cut(s)	123, 299
EciI	GGCGGA	1 cut(s)	294
Ecl136II	GAGCTC	2 cut(s)	70, 425
Eco130I	CCWWGG	3 cut(s)	222, 998, 1097
Eco24I	GRGCYC	2 cut(s)	72, 427
Eco47I	GGWCC	1 cut(s)	1142
Eco53kI	GAGCTC	2 cut(s)	70, 425
Eco57I	CTGAAG	1 cut(s)	196
EcoICRI	GAGCTC	2 cut(s)	70, 425
EcoRII	CCWGG	2 cut(s)	400, 1351
EcoT14I	CCWWGG	3 cut(s)	222, 998, 1097
EcoT22I	ATGCAT	2 cut(s)	955, 1249
EcoT38I	GRGCYC	2 cut(s)	72, 427
ErhI	CCWWGG	3 cut(s)	222, 998, 1097
Esp3I	CGTCTC	2 cut(s)	184, 538
FaeI	CATG	9 cut(s)	20, 226, 436, 648, 672, 715, 730, 957, 1370
FalI	AAGNNNNNCTT	4 cut(s)	95, 127, 1193, 1225
FatI	CATG	9 cut(s)	16, 222, 432, 644, 668, 711, 726, 953, 1366
FauI	CCCGC	1 cut(s)	884
Fnu4HI	GCNGC	7 cut(s)	441, 706, 780, 915, 1162, 1165, 1362
FokI	GGATG	4 cut(s)	211, 256, 1022, 1336
FriOI	GRGCYC	2 cut(s)	72, 427
Fsp4HI	GCNGC	7 cut(s)	441, 706, 780, 915, 1162, 1165, 1362
FspBI	CTAG	2 cut(s)	1098, 1445
GlaI	GCGC	1 cut(s)	291
GluI	GCNGC	7 cut(s)	441, 706, 780, 915, 1162, 1165, 1362
GsaI	CCCAGC	1 cut(s)	359
HaeIII	GGCC	2 cut(s)	748, 879
HgaI	GACGC	1 cut(s)	650
HhaI	GCGC	1 cut(s)	292
Hin1II	CATG	9 cut(s)	20, 226, 436, 648, 672, 715, 730, 957, 1370
Hin6I	GCGC	1 cut(s)	290
HinP1I	GCGC	1 cut(s)	290
HincII	GTYRAC	4 cut(s)	247, 339, 775, 1387
HindII	GTYRAC	4 cut(s)	247, 339, 775, 1387
HindIII	AAGCTT	2 cut(s)	58, 109
HinfI	GANTC	3 cut(s)	332, 551, 687
HpaI	GTTAAC	1 cut(s)	339
Hpy166II	GTNNAC	7 cut(s)	143, 247, 339, 775, 982, 984, 1387
Hpy188I	TCNGA	7 cut(s)	94, 127, 215, 543, 589, 821, 831
Hpy188III	TCNNGA	3 cut(s)	365, 712, 1376
Hpy8I	GTNNAC	7 cut(s)	143, 247, 339, 775, 982, 984, 1387
Hpy99I	CGWCG	2 cut(s)	98, 150
HpyAV	CCTTC	6 cut(s)	128, 574, 968, 1171, 1418, 1422
HpyCH4III	ACNGT	1 cut(s)	244
HpyCH4IV	ACGT	4 cut(s)	96, 139, 1425, 1435
HpyF10VI	GCNNNNNNNGC	3 cut(s)	923, 928, 1367
HpyF3I	CTNAG	2 cut(s)	9, 1328
HpySE526I	ACGT	4 cut(s)	96, 139, 1425, 1435
Hsp92II	CATG	9 cut(s)	20, 226, 436, 648, 672, 715, 730, 957, 1370
HspAI	GCGC	1 cut(s)	290
KspAI	GTTAAC	1 cut(s)	339
KspI	CCGCGG	1 cut(s)	918
Kzo9I	GATC	3 cut(s)	347, 490, 1403
LguI	GCTCTTC	1 cut(s)	299
LmnI	GCTCC	4 cut(s)	422, 1255, 1266, 1375
Lsp1109I	GCAGC	3 cut(s)	1151, 1173, 1373
LweI	GCATC	3 cut(s)	278, 625, 1314
MaeI	CTAG	2 cut(s)	1098, 1445
MaeII	ACGT	4 cut(s)	96, 139, 1425, 1435
MaeIII	GTNAC	2 cut(s)	23, 484
MalI	GATC	3 cut(s)	349, 492, 1405
MboI	GATC	3 cut(s)	347, 490, 1403
MboII	GAAGA	6 cut(s)	62, 140, 166, 202, 286, 511
MflI	RGATCY	1 cut(s)	1403
MhlI	GDGCHC	2 cut(s)	72, 427
MluCI	AATT	7 cut(s)	28, 380, 506, 825, 1048, 1347, 1453
MlyI	GAGTC	1 cut(s)	681
MmeI	TCCRAC	2 cut(s)	117, 951
Mph1103I	ATGCAT	2 cut(s)	955, 1249
MroXI	GAANNNNTTC	2 cut(s)	789, 1208
MseI	TTAA	7 cut(s)	309, 338, 474, 839, 1131, 1283, 1468
MslI	CAYNNNNRTG	1 cut(s)	30
MspA1I	CMGCKG	4 cut(s)	705, 782, 917, 1164
MspR9I	CCNGG	2 cut(s)	402, 1353
Mva1269I	GAATGC	1 cut(s)	862
MvaI	CCWGG	2 cut(s)	402, 1353
MvnI	CGCG	2 cut(s)	290, 917
MwoI	GCNNNNNNNGC	3 cut(s)	923, 928, 1367
NcoI	CCATGG	1 cut(s)	222
NdeII	GATC	3 cut(s)	347, 490, 1403
NlaIII	CATG	9 cut(s)	20, 226, 436, 648, 672, 715, 730, 957, 1370
NlaIV	GGNNCC	5 cut(s)	622, 749, 1095, 1194, 1405
NmeAIII	GCCGAG	1 cut(s)	669
NmuCI	GTSAC	2 cut(s)	23, 484
NsiI	ATGCAT	2 cut(s)	955, 1249
NspI	RCATGY	2 cut(s)	20, 730
PaeI	GCATGC	1 cut(s)	730
PagI	TCATGA	1 cut(s)	711
PciI	ACATGT	1 cut(s)	16
PciSI	GCTCTTC	1 cut(s)	299
PcsI	WCGNNNNNNNCGW	1 cut(s)	145
PctI	GAATGC	1 cut(s)	862
PdmI	GAANNNNTTC	2 cut(s)	789, 1208
PfeI	GAWTC	2 cut(s)	332, 551
PflMI	CCANNNNNTGG	1 cut(s)	886
PfoI	TCCNGGA	1 cut(s)	1351
PkrI	GCNGC	7 cut(s)	442, 707, 781, 916, 1163, 1166, 1363
PleI	GAGTC	1 cut(s)	681
PpsI	GAGTC	1 cut(s)	681
PscI	ACATGT	1 cut(s)	16
Psp124BI	GAGCTC	2 cut(s)	72, 427
Psp6I	CCWGG	2 cut(s)	400, 1351
PspFI	CCCAGC	1 cut(s)	355
PspGI	CCWGG	2 cut(s)	400, 1351
PspN4I	GGNNCC	5 cut(s)	622, 749, 1095, 1194, 1405
PspPI	GGNCC	2 cut(s)	747, 1142
PsrI	GAACNNNNNNTAC	2 cut(s)	223, 255
PstNI	CAGNNNCTG	2 cut(s)	744, 1164
PsuI	RGATCY	1 cut(s)	1403
PvuII	CAGCTG	1 cut(s)	1164
RsaI	GTAC	1 cut(s)	983
RsaNI	GTAC	1 cut(s)	982
RseI	CAYNNNNRTG	1 cut(s)	30
SacI	GAGCTC	2 cut(s)	72, 427
SacII	CCGCGG	1 cut(s)	918
SapI	GCTCTTC	1 cut(s)	299
SaqAI	TTAA	7 cut(s)	309, 338, 474, 839, 1131, 1283, 1468
SatI	GCNGC	7 cut(s)	441, 706, 780, 915, 1162, 1165, 1362
Sau3AI	GATC	3 cut(s)	347, 490, 1403
Sau96I	GGNCC	2 cut(s)	747, 1142
SchI	GAGTC	1 cut(s)	681
ScrFI	CCNGG	2 cut(s)	402, 1353
SduI	GDGCHC	2 cut(s)	72, 427
SfaNI	GCATC	3 cut(s)	278, 625, 1314
Sfr303I	CCGCGG	1 cut(s)	918
SgrBI	CCGCGG	1 cut(s)	918
SinI	GGWCC	1 cut(s)	1142
SmiMI	CAYNNNNRTG	1 cut(s)	30
SmlI	CTYRAG	2 cut(s)	41, 1374
SmoI	CTYRAG	2 cut(s)	41, 1374
SphI	GCATGC	1 cut(s)	730
Sse9I	AATT	7 cut(s)	28, 380, 506, 825, 1048, 1347, 1453
SsiI	CCGC	8 cut(s)	279, 440, 705, 780, 891, 915, 917, 1295
SspMI	CTAG	2 cut(s)	1098, 1445
SstI	GAGCTC	2 cut(s)	72, 427
StyD4I	CCNGG	2 cut(s)	400, 1351
StyI	CCWWGG	3 cut(s)	222, 998, 1097
TaaI	ACNGT	1 cut(s)	244
TaiI	ACGT	4 cut(s)	99, 142, 1428, 1438
TaqI	TCGA	5 cut(s)	66, 229, 660, 1396, 1464
TasI	AATT	7 cut(s)	28, 380, 506, 825, 1048, 1347, 1453
TatI	WGTACW	1 cut(s)	981
TauI	GCSGC	4 cut(s)	443, 708, 782, 917
TfiI	GAWTC	2 cut(s)	332, 551
Tru1I	TTAA	7 cut(s)	309, 338, 474, 839, 1131, 1283, 1468
Tru9I	TTAA	7 cut(s)	309, 338, 474, 839, 1131, 1283, 1468
TscAI	CASTG	2 cut(s)	357, 500
TseFI	GTSAC	2 cut(s)	23, 484
TseI	GCWGC	3 cut(s)	1161, 1164, 1361
Tsp45I	GTSAC	2 cut(s)	23, 484
TspDTI	ATGAA	8 cut(s)	52, 103, 518, 700, 728, 856, 1216, 1261
TspGWI	ACGGA	2 cut(s)	503, 668
TspRI	CASTG	2 cut(s)	357, 500
Van91I	CCANNNNNTGG	1 cut(s)	886
VpaK11BI	GGWCC	1 cut(s)	1142
XapI	RAATTY	2 cut(s)	506, 825
XceI	RCATGY	2 cut(s)	20, 730
XcmI	CCANNNNNNNNNTGG	3 cut(s)	352, 601, 741
XmaJI	CCTAGG	1 cut(s)	1097
XmnI	GAANNNNTTC	2 cut(s)	789, 1208
XspI	CTAG	2 cut(s)	1098, 1445
Zsp2I	ATGCAT	2 cut(s)	955, 1249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.