MD15G1081300.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005988
Physical Location & Seq
Forward (+)
5586503 .. 5589556
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1081300.v1.1.491

Sequence Viewer

Length: 1419 bp
ATGGCTTCCAAGGGACCTGTCTGTCTCAGTTATGTATTTCTCAAGCCCGAAGAAGCAACTTTCATCGAGCTCATTCGCCTCCTCTTTTCCTCTGACATAGAAAAGAGAAAATTCATCACCTCCTCGGCAGTAAAAAAACTTGACTTCAAACGGAGGTTGATAATCTTCCTCTCTGCGGTGATTCAATGCATCCTCCTTCTCTTAAGAAAACCCATGGCTTGGCTTGGGGGTGTTCTGGAGAAGTGGCTAAATCTTGTATCAAGCAACGGTGGATTGCTCTTGCTCATCCTAAACTGGCTAAAAGGAAAGGTGGTGATACCAGAATCATCATCAACCAGATCCGTGCTTGGATTTCTTGACACACGAATTGAATTAGACGACAACATCAAACCTGACGACACAAGATATAACGGGTCGCTGTCCATGATGGCAGCCAAATTGTCGTACGAGAATGAAGAATTCATTGAAAAAGTTGTCAGTGAATACTGGAAGATGAAACTCGTGGCGTTCCACAAATTTTGGAATGGTTACGAGAAACAAGAATCAACCCAAGCCTTCGTGTTCCAAGACACAACATCTGACCCCAACTTGGTTTGGGTCGCATTTAGAGGCACTGAACCATTCAGCGCCGACGATTGGAGGACAGATTTTGACATCTCATGGTGCGAGTTTGCCGGAATCGGCAAGACCCACGGAGGCTTTATGAAAGCTCTCGGCATGCAAACAGAGAAAAGCTGGCCCCTCGAACCCGAAACACAAGATCATGAGTTTGCTTACTACAAAATCAGACAAATTCTGATGGATTTATTGCAAGAAAACAAGAATGCCAAATTTATATTGACAGGTCACAGCTTAGGAGGGGCACTGGCGGTGCTGTTTGCATCGGTGCTGGCAATGCATGACTTCGAGGATAAATATGCCTGGTTGATGGAGAGGTTGGAGGGGGTGTACACTTTTGGACAGCCGAGGGTGGGGGATGAGAAATTTGCGGGCTATGTAACGAAGAAGATGAAAAATTATGCTGTGAGGTATATGAGGTATGTTTATTCCAGTGACTTGGTGCCTAGGGTACCATTTGATGATACAACTCTATTGTTTAAGCACGTTGCTCCATGCCTTTATTTCAACAGCTTCTATAAAGGGAAGGAGTTGGAGGAGGCACCCAACAAGAACTACTTCAATTTGCTTTTCTTCATACCGATGCGGTTAAATGCATCGTGGGAGCTGATTAGGAGTTTCATGCTCCCATTAATAAAGGGTTCAGAATACAAGGAAAGCTGCCTTATGATAATGACTAGGGTTTTTGGTTTAATATTTCCTGGATTGGTAGCTCATGCTCCTCAAGATTATGTCAATGTTACTCGATTGGGATCGATCCGTCCTACATGCCACTCTCAAAAGCTTACCTCCAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

473

Amino Acids

54.43

Weight (kDa)

8.74

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 200 - 360 7.7e-33 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 21
Acc65I GGTACC 1 cut(s) 1069
AccB1I GGYRCC 3 cut(s) 1060, 1069, 1159
AccB7I CCANNNNNTGG 1 cut(s) 219
AciI CCGC 4 cut(s) 176, 869, 989, 1204
AclWI GGATC 3 cut(s) 333, 1369, 1378
AcsI RAATTY 6 cut(s) 110, 458, 515, 792, 830, 983
AfaI GTAC 3 cut(s) 446, 950, 1071
AfiI CCNNNNNNNGG 5 cut(s) 175, 219, 589, 636, 971
AflII CTTAAG 1 cut(s) 202
AgsI TTSAA 6 cut(s) 148, 185, 371, 467, 1126, 1180
AjnI CCWGG 2 cut(s) 920, 1318
AluBI AGCT 9 cut(s) 70, 710, 735, 852, 1131, 1225, 1278, 1331, 1402
AluI AGCT 9 cut(s) 70, 710, 735, 852, 1131, 1225, 1278, 1331, 1402
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 3 cut(s) 333, 1369, 1378
AoxI GGCC 1 cut(s) 737
ApeKI GCWGC 2 cut(s) 431, 1278
ApoI RAATTY 6 cut(s) 110, 458, 515, 792, 830, 983
AseI ATTAAT 1 cut(s) 1250
Asp700I GAANNNNTTC 1 cut(s) 1175
Asp718I GGTACC 1 cut(s) 1069
AspA2I CCTAGG 1 cut(s) 1064
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 2 cut(s) 14, 738
AsuHPI GGTGA 3 cut(s) 109, 190, 325
AvaII GGWCC 1 cut(s) 14
AvrII CCTAGG 1 cut(s) 1064
BaeGI GKGCMC 1 cut(s) 865
BanI GGYRCC 3 cut(s) 1060, 1069, 1159
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 443, 1265
BccI CCATC 3 cut(s) 421, 793, 922
BciT130I CCWGG 2 cut(s) 922, 1320
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 1065, 1296
BfoI RGCGCY 1 cut(s) 630
BfrI CTTAAG 1 cut(s) 202
BisI GCNGC 2 cut(s) 432, 1279
BlnI CCTAGG 1 cut(s) 1064
BlsI GCNGC 2 cut(s) 433, 1280
Bme1390I CCNGG 2 cut(s) 922, 1320
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 2 cut(s) 14, 738
BmiI GGNNCC 5 cut(s) 15, 740, 1062, 1071, 1161
BmrFI CCNGG 2 cut(s) 922, 1320
BmsI GCATC 4 cut(s) 198, 890, 1191, 1223
BpmI CTGGAG 2 cut(s) 257, 1393
Bpu10I CCTNAGC 1 cut(s) 853
BpuEI CTTGAG 2 cut(s) 26, 1326
Bsa29I ATCGAT 1 cut(s) 1373
BsaBI GATNNNNATC 1 cut(s) 1369
BsaJI CCNNGG 6 cut(s) 9, 123, 213, 691, 965, 1064
BsaXI ACNNNNNCTCC 2 cut(s) 932, 962
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 219, 589, 636, 971
Bse1I ACTGG 4 cut(s) 299, 491, 870, 1050
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 1369
BseBI CCWGG 2 cut(s) 922, 1320
BseCI ATCGAT 1 cut(s) 1373
BseDI CCNNGG 6 cut(s) 9, 123, 213, 691, 965, 1064
BseGI GGATG 3 cut(s) 189, 285, 982
BseJI GATNNNNATC 1 cut(s) 1369
BseLI CCNNNNNNNGG 5 cut(s) 175, 219, 589, 636, 971
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 4 cut(s) 299, 491, 870, 1050
BseRI GAGGAG 4 cut(s) 71, 112, 1169, 1329
BseSI GKGCMC 1 cut(s) 865
BseXI GCAGC 2 cut(s) 443, 1265
BshFI GGCC 1 cut(s) 739
BshNI GGYRCC 3 cut(s) 1060, 1069, 1159
BshVI ATCGAT 1 cut(s) 1373
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 675
BsiWI CGTACG 1 cut(s) 444
BslFI GGGAC 1 cut(s) 27
BslI CCNNNNNNNGG 5 cut(s) 175, 219, 589, 636, 971
BsmAI GTCTC 1 cut(s) 29
BsmFI GGGAC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 1 cut(s) 739
Bsp1286I GDGCHC 2 cut(s) 72, 865
Bsp1407I TGTACA 1 cut(s) 948
Bsp143I GATC 4 cut(s) 338, 760, 1370, 1374
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 4 cut(s) 176, 869, 989, 1204
BspANI GGCC 1 cut(s) 739
BspCNI CTCAG 1 cut(s) 39
BspDI ATCGAT 1 cut(s) 1373
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 5 cut(s) 15, 740, 1062, 1071, 1161
BspPI GGATC 3 cut(s) 333, 1369, 1378
BspT107I GGYRCC 3 cut(s) 1060, 1069, 1159
BspTI CTTAAG 1 cut(s) 202
BsrDI GCAATG 1 cut(s) 900
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 4 cut(s) 299, 491, 870, 1050
BssECI CCNNGG 6 cut(s) 9, 123, 213, 691, 965, 1064
BssMI GATC 4 cut(s) 338, 760, 1370, 1374
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 3 cut(s) 9, 213, 1064
Bst2BI CACGAG 1 cut(s) 500
Bst2UI CCWGG 2 cut(s) 922, 1320
Bst4CI ACNGT 1 cut(s) 269
BstAFI CTTAAG 1 cut(s) 202
BstAUI TGTACA 1 cut(s) 948
BstC8I GCNNGC 4 cut(s) 719, 737, 891, 991
BstDEI CTNAG 2 cut(s) 26, 853
BstDSI CCRYGG 2 cut(s) 213, 691
BstF5I GGATG 3 cut(s) 189, 285, 982
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 4 cut(s) 341, 763, 1373, 1377
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 4 cut(s) 338, 760, 1370, 1374
BstMWI GCNNNNNNNGC 1 cut(s) 895
BstNI CCWGG 2 cut(s) 922, 1320
BstNSI RCATGY 2 cut(s) 721, 1389
BstSCI CCNGG 2 cut(s) 920, 1318
BstSLI GKGCMC 1 cut(s) 865
BstV1I GCAGC 2 cut(s) 443, 1265
BstX2I RGATCY 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 1057
BstYI RGATCY 1 cut(s) 338
Bsu15I ATCGAT 1 cut(s) 1373
BsuRI GGCC 1 cut(s) 739
BsuTUI ATCGAT 1 cut(s) 1373
BtgI CCRYGG 2 cut(s) 213, 691
BtsCI GGATG 3 cut(s) 189, 285, 982
BtsIMutI CAGTG 4 cut(s) 484, 612, 863, 1057
Cac8I GCNNGC 4 cut(s) 719, 737, 891, 991
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 2 cut(s) 14, 738
ClaI ATCGAT 1 cut(s) 1373
Csp6I GTAC 3 cut(s) 445, 949, 1070
CspCI CAANNNNNGTGG 2 cut(s) 500, 535
CviQI GTAC 3 cut(s) 445, 949, 1070
DdeI CTNAG 2 cut(s) 26, 853
DpnI GATC 4 cut(s) 340, 762, 1372, 1376
DpnII GATC 4 cut(s) 338, 760, 1370, 1374
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 9, 213, 1064
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 14
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 920, 1318
EcoT14I CCWWGG 3 cut(s) 9, 213, 1064
EcoT22I ATGCAT 3 cut(s) 191, 900, 1216
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 9, 213, 1064
FalI AAGNNNNNCTT 2 cut(s) 1160, 1192
FaqI GGGAC 1 cut(s) 27
FauI CCCGC 1 cut(s) 982
Fnu4HI GCNGC 2 cut(s) 432, 1279
FokI GGATG 3 cut(s) 176, 272, 989
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 432, 1279
FspBI CTAG 2 cut(s) 1065, 1296
GlaI GCGC 1 cut(s) 628
GluI GCNGC 2 cut(s) 432, 1279
GsuI CTGGAG 2 cut(s) 257, 1393
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 1 cut(s) 739
HapII CCGG 1 cut(s) 675
HhaI GCGC 1 cut(s) 629
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HindIII AAGCTT 1 cut(s) 1400
HinfI GANTC 4 cut(s) 181, 323, 542, 678
HpaII CCGG 1 cut(s) 675
HphI GGTGA 3 cut(s) 109, 190, 325
Hpy166II GTNNAC 2 cut(s) 949, 951
Hpy188I TCNGA 5 cut(s) 94, 580, 788, 798, 1264
Hpy188III TCNNGA 5 cut(s) 236, 356, 764, 1343, 1410
Hpy8I GTNNAC 2 cut(s) 949, 951
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 3 cut(s) 206, 565, 1138
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 1104
HpyCH4V TGCA 6 cut(s) 189, 721, 811, 881, 898, 1214
HpyF10VI GCNNNNNNNGC 1 cut(s) 895
HpyF3I CTNAG 2 cut(s) 26, 853
HpySE526I ACGT 1 cut(s) 1104
HspAI GCGC 1 cut(s) 627
KpnI GGTACC 1 cut(s) 1073
Kzo9I GATC 4 cut(s) 338, 760, 1370, 1374
LmnI GCTCC 4 cut(s) 1114, 1222, 1248, 1342
Lsp1109I GCAGC 2 cut(s) 443, 1265
LweI GCATC 4 cut(s) 198, 890, 1191, 1223
MaeI CTAG 2 cut(s) 1065, 1296
MaeII ACGT 1 cut(s) 1104
MaeIII GTNAC 5 cut(s) 527, 845, 997, 1052, 1357
MalI GATC 4 cut(s) 340, 762, 1372, 1376
MboI GATC 4 cut(s) 338, 760, 1370, 1374
MboII GAAGA 7 cut(s) 62, 157, 467, 502, 1015, 1018, 1183
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 2 cut(s) 72, 865
MmeI TCCRAC 2 cut(s) 918, 1131
Mph1103I ATGCAT 3 cut(s) 191, 900, 1216
MroXI GAANNNNTTC 1 cut(s) 1175
MseI TTAA 5 cut(s) 203, 1098, 1208, 1250, 1310
MslI CAYNNNNRTG 1 cut(s) 1199
MspCI CTTAAG 1 cut(s) 202
MspI CCGG 1 cut(s) 675
MspR9I CCNGG 2 cut(s) 922, 1320
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 2 cut(s) 922, 1320
MwoI GCNNNNNNNGC 1 cut(s) 895
NcoI CCATGG 1 cut(s) 213
NdeII GATC 4 cut(s) 338, 760, 1370, 1374
NlaIV GGNNCC 5 cut(s) 15, 740, 1062, 1071, 1161
NmeAIII GCCGAG 3 cut(s) 104, 693, 990
NmuCI GTSAC 2 cut(s) 845, 1052
NsiI ATGCAT 3 cut(s) 191, 900, 1216
NspI RCATGY 2 cut(s) 721, 1389
PaeI GCATGC 1 cut(s) 721
PagI TCATGA 1 cut(s) 763
PctI GAATGC 1 cut(s) 829
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 4 cut(s) 181, 323, 542, 678
Pfl23II CGTACG 1 cut(s) 444
PflMI CCANNNNNTGG 1 cut(s) 219
PfoI TCCNGGA 1 cut(s) 1318
PkrI GCNGC 2 cut(s) 433, 1280
PpuMI RGGWCCY 1 cut(s) 14
PshBI ATTAAT 1 cut(s) 1250
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 14
Psp6I CCWGG 2 cut(s) 920, 1318
PspGI CCWGG 2 cut(s) 920, 1318
PspLI CGTACG 1 cut(s) 444
PspN4I GGNNCC 5 cut(s) 15, 740, 1062, 1071, 1161
PspPI GGNCC 2 cut(s) 14, 738
PspPPI RGGWCCY 1 cut(s) 14
PsuI RGATCY 1 cut(s) 338
RsaI GTAC 3 cut(s) 446, 950, 1071
RsaNI GTAC 3 cut(s) 445, 949, 1070
RseI CAYNNNNRTG 1 cut(s) 1199
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 5 cut(s) 203, 1098, 1208, 1250, 1310
SatI GCNGC 2 cut(s) 432, 1279
Sau3AI GATC 4 cut(s) 338, 760, 1370, 1374
Sau96I GGNCC 2 cut(s) 14, 738
ScrFI CCNGG 2 cut(s) 922, 1320
SduI GDGCHC 2 cut(s) 72, 865
SfaNI GCATC 4 cut(s) 198, 890, 1191, 1223
SinI GGWCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 1199
SmlI CTYRAG 3 cut(s) 41, 202, 1341
SmoI CTYRAG 3 cut(s) 41, 202, 1341
SphI GCATGC 1 cut(s) 721
SsiI CCGC 4 cut(s) 176, 869, 989, 1204
SspI AATATT 1 cut(s) 1314
SspMI CTAG 2 cut(s) 1065, 1296
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 920, 1318
StyI CCWWGG 3 cut(s) 9, 213, 1064
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 1107
TaqI TCGA 5 cut(s) 66, 744, 906, 1363, 1373
TatI WGTACW 1 cut(s) 948
TfiI GAWTC 4 cut(s) 181, 323, 542, 678
Tru1I TTAA 5 cut(s) 203, 1098, 1208, 1250, 1310
Tru9I TTAA 5 cut(s) 203, 1098, 1208, 1250, 1310
TscAI CASTG 4 cut(s) 484, 619, 870, 1057
TseFI GTSAC 2 cut(s) 845, 1052
TseI GCWGC 2 cut(s) 431, 1278
Tsp45I GTSAC 2 cut(s) 845, 1052
TspDTI ATGAA 9 cut(s) 52, 103, 451, 468, 509, 719, 1025, 1183, 1228
TspGWI ACGGA 4 cut(s) 166, 331, 708, 1367
TspRI CASTG 4 cut(s) 484, 619, 870, 1057
Van91I CCANNNNNTGG 1 cut(s) 219
Vha464I CTTAAG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 14
VspI ATTAAT 1 cut(s) 1250
XapI RAATTY 6 cut(s) 110, 458, 515, 792, 830, 983
XceI RCATGY 2 cut(s) 721, 1389
XmaJI CCTAGG 1 cut(s) 1064
XmnI GAANNNNTTC 1 cut(s) 1175
XspI CTAG 2 cut(s) 1065, 1296
Zsp2I ATGCAT 3 cut(s) 191, 900, 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.