FvH4_2g34290

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005988
Physical Location & Seq
Forward (+)
25513137 .. 25517830
4694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34290.t1

Sequence Viewer

Length: 1425 bp
ATGGCTTCTAAGGAACATGTATGTGACAATTACTTTAGTTTGAAGCCAGAAGAAGCAACGCTCATCGAGCTTCTCCGACTTCTGTTTTCCTCCGATTTAGACAGCAGAAAATTCATCGAGTCCTCGGGAGAGGTTTGGAAGTTAGAACGAAGGTGGATACTCCTCGTCTCTGTTGTAGTGCAACGGTTCTTCCTCTTCTGCAGTGTTCCTCTGGCGAGAATAGGAGACGTGCTGCAGAAATGGCTCAACCTTGTTTCGATCAACGGCGGACCACTCATGCTCTTGTTAAACCGGCTAAGAGGAAAGGTGATGGAGCCGGATTCGTTATCATCGACATCGGCGGTTGGAAATCTGGACAGGAGAATTGAATTAGACAAGAGCATCAAACCTGAGAATCCAAGATACAAAGCGGCTCTCTCTATGATGGCTGCTAAGTTGTCATACGAGAACAAAGACCTCGTTCAATCCGTCGTTAACCATCACTGGAAGATGAAATTCGTGGGATTCTACAACTTTCGCAATGGTTACCAAGGACGAGATTCAACACAAGCCTTCTTGTTCAAAGAGAGACAGTCTGACCCTAACTTGATTGTGGTGGCATTTAGAGGCACTTCCCCATTTAATGCCGATGCATGGAGGACCGATTTCGACCTCTCATGGTGCGAGTTCAACAAAGTCGGTAAGACGCACAGTGGCTTCATGAAAGCTCTCGGCATGCAACCAAACAATCGGTGGCCACTTGAACTAGAAACTGATCAACCACAAGGCAGCAGTGACCAACATCCTCAGTTTGCTTACTACACAATCAGAAAAATGCTGGTAGATTTGCTGCAAGAGGATGTGAATGCAAAGTTTATATTGACGGGGCACAGCTTGGGCGGCGCGCTAGCGATTTTGTTTGCAGGTGTGCTAGCAATGCATGATCATGAGTATGCATGGTTGCTGGAGAGGATGGAGGGGGTTTACACGTTCGGACAGCCTAGGGTTGGAGATGAGAAGTTTGGGGAGTTTATGAAGGAAAAGTTTAGGAAGTATGATGTGAGGTATATGAGGTATGTCTACAGCAATGACCTGGTGCCTAGAATACCTTATGATGATAAAGCCCTCTTGTTCAAGCACTTCGGGCCAAGTCTGTACTACGACAGCTGCTACAGAGGAAAGGTTTTGGAGGAGGAACCCAACAAGAACTACTTCAATTTGCTATGGTTCATACCGAAGAGGCTGAACGCAGTGTGGGAGCTGATAAGGAGCTTCATACTCCCTTGGGTGAAGGGTTCAGAATACAAGGAAGGCTGGTTTATGAGATTTTGGAGGCTAGTCGGATTAGTAATTCCAGGATTGGGAGCCCATGCTCCTCAAGATTATGTTAATCTTACAAGATTGGGAACCCTGCTTCTTCCTCAAGAAAGTCCTAAAGTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

475

Amino Acids

55.1

Weight (kDa)

8.85

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 198 - 365 1.5e-31 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 893
Acc36I ACCTGC 1 cut(s) 893
AccB1I GGYRCC 1 cut(s) 1075
AccI GTMKAC 1 cut(s) 1059
AccII CGCG 1 cut(s) 884
AciI CCGC 4 cut(s) 267, 341, 410, 879
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 2 cut(s) 110, 494
AfaI GTAC 1 cut(s) 1136
AfiI CCNNNNNNNGG 3 cut(s) 633, 986, 1340
AflIII ACRYGT 2 cut(s) 16, 966
AgsI TTSAA 9 cut(s) 43, 368, 464, 543, 562, 670, 743, 1114, 1195
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 1071, 1333
AluBI AGCT 6 cut(s) 70, 707, 873, 1146, 1240, 1251
AluI AGCT 6 cut(s) 70, 707, 873, 1146, 1240, 1251
Alw26I GTCTC 3 cut(s) 172, 219, 562
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 2 cut(s) 734, 1124
ApeKI GCWGC 5 cut(s) 232, 428, 768, 829, 1146
ApoI RAATTY 2 cut(s) 110, 494
Asp700I GAANNNNTTC 1 cut(s) 1190
AspA2I CCTAGG 1 cut(s) 980
AspLEI GCGC 2 cut(s) 884, 886
AspS9I GGNCC 3 cut(s) 269, 639, 1124
AsuHPI GGTGA 2 cut(s) 319, 1279
AsuNHI GCTAGC 2 cut(s) 886, 910
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 2 cut(s) 269, 639
AvrII CCTAGG 1 cut(s) 980
BaeGI GKGCMC 1 cut(s) 870
BalI TGGCCA 1 cut(s) 736
BanI GGYRCC 1 cut(s) 1075
BanII GRGCYC 1 cut(s) 1348
BbvI GCAGC 5 cut(s) 219, 415, 780, 816, 1133
BccI CCATC 4 cut(s) 304, 418, 486, 946
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 2 cut(s) 1073, 1335
BciVI GTATCC 1 cut(s) 150
BclI TGATCA 2 cut(s) 754, 922
BcoDI GTCTC 3 cut(s) 172, 219, 562
BfaI CTAG 6 cut(s) 746, 887, 911, 981, 1080, 1316
BfmI CTRYAG 4 cut(s) 199, 233, 1060, 1150
BfuAI ACCTGC 1 cut(s) 893
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 7 cut(s) 233, 411, 429, 769, 830, 880, 1147
BlnI CCTAGG 1 cut(s) 980
BlsI GCNGC 7 cut(s) 234, 412, 430, 770, 831, 881, 1148
Bme1390I CCNGG 2 cut(s) 1073, 1335
Bme18I GGWCC 2 cut(s) 269, 639
BmeT110I CYCGRG 1 cut(s) 124
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 269, 639, 1124
BmiI GGNNCC 5 cut(s) 315, 1077, 1176, 1345, 1387
BmrFI CCNGG 2 cut(s) 1073, 1335
BmsI GCATC 2 cut(s) 390, 619
BmtI GCTAGC 2 cut(s) 890, 914
BpmI CTGGAG 1 cut(s) 965
BpuEI CTTGAG 2 cut(s) 1341, 1386
BsaJI CCNNGG 4 cut(s) 123, 529, 980, 1262
Bsc4I CCNNNNNNNGG 3 cut(s) 633, 986, 1340
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 3 cut(s) 526, 921, 1072
BseBI CCWGG 2 cut(s) 1073, 1335
BseDI CCNNGG 4 cut(s) 123, 529, 980, 1262
BseGI GGATG 3 cut(s) 781, 844, 957
BseLI CCNNNNNNNGG 3 cut(s) 633, 986, 1340
BseMI GCAATG 3 cut(s) 526, 921, 1072
BseMII CTCAG 2 cut(s) 381, 800
BseNI ACTGG 1 cut(s) 488
BsePI GCGCGC 1 cut(s) 882
BseRI GAGGAG 3 cut(s) 152, 1184, 1344
BseSI GKGCMC 1 cut(s) 870
BseXI GCAGC 5 cut(s) 219, 415, 780, 816, 1133
Bsh1236I CGCG 1 cut(s) 884
BshFI GGCC 2 cut(s) 736, 1126
BshNI GGYRCC 1 cut(s) 1075
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 2 cut(s) 292, 317
BslI CCNNNNNNNGG 3 cut(s) 633, 986, 1340
BsmAI GTCTC 3 cut(s) 172, 219, 562
BsmBI CGTCTC 2 cut(s) 172, 219
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 2 cut(s) 736, 1126
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 870, 1348
Bsp143I GATC 3 cut(s) 258, 754, 922
BspACI CCGC 4 cut(s) 267, 341, 410, 879
BspANI GGCC 2 cut(s) 736, 1126
BspCNI CTCAG 2 cut(s) 382, 799
BspFNI CGCG 1 cut(s) 884
BspHI TCATGA 2 cut(s) 699, 925
BspLI GGNNCC 5 cut(s) 315, 1077, 1176, 1345, 1387
BspMAI CTGCAG 2 cut(s) 203, 237
BspMI ACCTGC 1 cut(s) 893
BspOI GCTAGC 2 cut(s) 890, 914
BspT107I GGYRCC 1 cut(s) 1075
BsrDI GCAATG 3 cut(s) 526, 921, 1072
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 1 cut(s) 488
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 4 cut(s) 123, 529, 980, 1262
BssHII GCGCGC 1 cut(s) 882
BssMI GATC 3 cut(s) 258, 754, 922
BssT1I CCWWGG 3 cut(s) 529, 980, 1262
Bst2UI CCWGG 2 cut(s) 1073, 1335
Bst4CI ACNGT 3 cut(s) 186, 573, 692
Bst6I CTCTTC 2 cut(s) 200, 1211
BstC8I GCNNGC 4 cut(s) 716, 884, 888, 912
BstDEI CTNAG 5 cut(s) 9, 296, 390, 432, 786
BstEII GGTNACC 1 cut(s) 524
BstF5I GGATG 3 cut(s) 781, 844, 957
BstFNI CGCG 1 cut(s) 884
BstHHI GCGC 2 cut(s) 884, 886
BstKTI GATC 3 cut(s) 261, 757, 925
BstMAI GTCTC 3 cut(s) 172, 219, 562
BstMBI GATC 3 cut(s) 258, 754, 922
BstMWI GCNNNNNNNGC 5 cut(s) 67, 241, 879, 916, 1123
BstNI CCWGG 2 cut(s) 1073, 1335
BstNSI RCATGY 2 cut(s) 20, 718
BstPI GGTNACC 1 cut(s) 524
BstSCI CCNGG 2 cut(s) 1071, 1333
BstSFI CTRYAG 4 cut(s) 199, 233, 1060, 1150
BstSLI GKGCMC 1 cut(s) 870
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 5 cut(s) 219, 415, 780, 816, 1133
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 736, 1126
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 3 cut(s) 781, 844, 957
BtsI GCAGTG 3 cut(s) 208, 778, 1236
BtsIMutI CAGTG 5 cut(s) 208, 481, 697, 778, 1236
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 4 cut(s) 716, 884, 888, 912
CciI TCATGA 2 cut(s) 699, 925
CfoI GCGC 2 cut(s) 884, 886
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 269, 639, 1124
CseI GACGC 1 cut(s) 694
CsiI ACCWGGT 1 cut(s) 1071
Csp6I GTAC 1 cut(s) 1135
CviQI GTAC 1 cut(s) 1135
DdeI CTNAG 5 cut(s) 9, 296, 390, 432, 786
DpnI GATC 3 cut(s) 260, 756, 924
DpnII GATC 3 cut(s) 258, 754, 922
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 2 cut(s) 200, 1211
EarI CTCTTC 2 cut(s) 200, 1211
EciI GGCGGA 1 cut(s) 282
Eco130I CCWWGG 3 cut(s) 529, 980, 1262
Eco24I GRGCYC 1 cut(s) 1348
Eco47I GGWCC 2 cut(s) 269, 639
Eco88I CYCGRG 1 cut(s) 124
Eco91I GGTNACC 1 cut(s) 524
EcoO65I GGTNACC 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 1071, 1333
EcoT14I CCWWGG 3 cut(s) 529, 980, 1262
EcoT22I ATGCAT 3 cut(s) 634, 921, 937
EcoT38I GRGCYC 1 cut(s) 1348
ErhI CCWWGG 3 cut(s) 529, 980, 1262
Esp3I CGTCTC 2 cut(s) 172, 219
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FbaI TGATCA 2 cut(s) 754, 922
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 7 cut(s) 233, 411, 429, 769, 830, 880, 1147
FokI GGATG 3 cut(s) 768, 851, 964
FriOI GRGCYC 1 cut(s) 1348
Fsp4HI GCNGC 7 cut(s) 233, 411, 429, 769, 830, 880, 1147
FspBI CTAG 6 cut(s) 746, 887, 911, 981, 1080, 1316
GlaI GCGC 2 cut(s) 883, 885
GluI GCNGC 7 cut(s) 233, 411, 429, 769, 830, 880, 1147
GsuI CTGGAG 1 cut(s) 965
HaeIII GGCC 2 cut(s) 736, 1126
HapII CCGG 2 cut(s) 292, 317
HgaI GACGC 1 cut(s) 694
HhaI GCGC 2 cut(s) 884, 886
Hin6I GCGC 2 cut(s) 882, 884
HinP1I GCGC 2 cut(s) 882, 884
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 5 cut(s) 119, 320, 394, 504, 539
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 2 cut(s) 292, 317
HphI GGTGA 2 cut(s) 319, 1279
Hpy166II GTNNAC 3 cut(s) 475, 964, 1060
Hpy188I TCNGA 7 cut(s) 77, 94, 577, 809, 974, 1279, 1322
Hpy188III TCNNGA 6 cut(s) 126, 353, 700, 926, 1358, 1403
Hpy8I GTNNAC 3 cut(s) 475, 964, 1060
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 5 cut(s) 144, 562, 1009, 1264, 1283
HpyCH4III ACNGT 3 cut(s) 186, 573, 692
HpyCH4IV ACGT 2 cut(s) 228, 968
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 241, 879, 916, 1123
HpyF3I CTNAG 5 cut(s) 9, 296, 390, 432, 786
HpySE526I ACGT 2 cut(s) 228, 968
HspAI GCGC 2 cut(s) 882, 884
Ksp22I TGATCA 2 cut(s) 754, 922
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 3 cut(s) 258, 754, 922
LmnI GCTCC 5 cut(s) 313, 1237, 1248, 1343, 1357
Lsp1109I GCAGC 5 cut(s) 219, 415, 780, 816, 1133
LweI GCATC 2 cut(s) 390, 619
MabI ACCWGGT 1 cut(s) 1071
MaeI CTAG 6 cut(s) 746, 887, 911, 981, 1080, 1316
MaeII ACGT 2 cut(s) 228, 968
MaeIII GTNAC 3 cut(s) 23, 524, 773
MalI GATC 3 cut(s) 260, 756, 924
MboI GATC 3 cut(s) 258, 754, 922
MboII GAAGA 6 cut(s) 62, 181, 187, 499, 1228, 1388
MhlI GDGCHC 2 cut(s) 870, 1348
MlsI TGGCCA 1 cut(s) 736
MluCI AATT 7 cut(s) 28, 110, 363, 368, 494, 1195, 1329
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 4 cut(s) 100, 325, 967, 1300
Mox20I TGGCCA 1 cut(s) 736
Mph1103I ATGCAT 3 cut(s) 634, 921, 937
MroXI GAANNNNTTC 1 cut(s) 1190
MscI TGGCCA 1 cut(s) 736
MseI TTAA 4 cut(s) 287, 474, 621, 1368
MslI CAYNNNNRTG 3 cut(s) 21, 924, 930
Msp20I TGGCCA 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 1146
MspI CCGG 2 cut(s) 292, 317
MspR9I CCNGG 2 cut(s) 1073, 1335
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 2 cut(s) 1073, 1335
MvnI CGCG 1 cut(s) 884
MwoI GCNNNNNNNGC 5 cut(s) 67, 241, 879, 916, 1123
NdeII GATC 3 cut(s) 258, 754, 922
NheI GCTAGC 2 cut(s) 886, 910
NlaIV GGNNCC 5 cut(s) 315, 1077, 1176, 1345, 1387
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 2 cut(s) 23, 773
NsiI ATGCAT 3 cut(s) 634, 921, 937
NspI RCATGY 2 cut(s) 20, 718
PaeI GCATGC 1 cut(s) 718
PagI TCATGA 2 cut(s) 699, 925
PaqCI CACCTGC 1 cut(s) 893
PauI GCGCGC 1 cut(s) 882
PciI ACATGT 1 cut(s) 16
PcsI WCGNNNNNNNCGW 2 cut(s) 329, 465
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 1190
PfeI GAWTC 4 cut(s) 320, 394, 504, 539
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 7 cut(s) 234, 412, 430, 770, 831, 881, 1148
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PscI ACATGT 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 1071, 1333
PspEI GGTNACC 1 cut(s) 524
PspGI CCWGG 2 cut(s) 1071, 1333
PspN4I GGNNCC 5 cut(s) 315, 1077, 1176, 1345, 1387
PspPI GGNCC 3 cut(s) 269, 639, 1124
PstI CTGCAG 2 cut(s) 203, 237
PteI GCGCGC 1 cut(s) 882
PvuII CAGCTG 1 cut(s) 1146
RsaI GTAC 1 cut(s) 1136
RsaNI GTAC 1 cut(s) 1135
RseI CAYNNNNRTG 3 cut(s) 21, 924, 930
SaqAI TTAA 4 cut(s) 287, 474, 621, 1368
SatI GCNGC 7 cut(s) 233, 411, 429, 769, 830, 880, 1147
Sau3AI GATC 3 cut(s) 258, 754, 922
Sau96I GGNCC 3 cut(s) 269, 639, 1124
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 1073, 1335
SduI GDGCHC 2 cut(s) 870, 1348
SexAI ACCWGGT 1 cut(s) 1071
SfaNI GCATC 2 cut(s) 390, 619
SfcI CTRYAG 4 cut(s) 199, 233, 1060, 1150
SinI GGWCC 2 cut(s) 269, 639
SmiMI CAYNNNNRTG 3 cut(s) 21, 924, 930
SmlI CTYRAG 2 cut(s) 1356, 1401
SmoI CTYRAG 2 cut(s) 1356, 1401
SphI GCATGC 1 cut(s) 718
Sse9I AATT 7 cut(s) 28, 110, 363, 368, 494, 1195, 1329
SsiI CCGC 4 cut(s) 267, 341, 410, 879
SspMI CTAG 6 cut(s) 746, 887, 911, 981, 1080, 1316
StyD4I CCNGG 2 cut(s) 1071, 1333
StyI CCWWGG 3 cut(s) 529, 980, 1262
TaaI ACNGT 3 cut(s) 186, 573, 692
TaiI ACGT 2 cut(s) 231, 971
TaqI TCGA 6 cut(s) 66, 117, 257, 332, 648, 1419
TaqII GACCGA 1 cut(s) 656
TasI AATT 7 cut(s) 28, 110, 363, 368, 494, 1195, 1329
TatI WGTACW 1 cut(s) 1134
TauI GCSGC 2 cut(s) 413, 882
TfiI GAWTC 4 cut(s) 320, 394, 504, 539
Tru1I TTAA 4 cut(s) 287, 474, 621, 1368
Tru9I TTAA 4 cut(s) 287, 474, 621, 1368
TscAI CASTG 5 cut(s) 208, 488, 697, 778, 1236
TseFI GTSAC 2 cut(s) 23, 773
TseI GCWGC 5 cut(s) 232, 428, 768, 829, 1146
Tsp45I GTSAC 2 cut(s) 23, 773
TspDTI ATGAA 7 cut(s) 103, 506, 688, 716, 1028, 1198, 1243
TspGWI ACGGA 1 cut(s) 457
TspRI CASTG 5 cut(s) 208, 488, 697, 778, 1236
VpaK11BI GGWCC 2 cut(s) 269, 639
XapI RAATTY 2 cut(s) 110, 494
XceI RCATGY 2 cut(s) 20, 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 729
XmaJI CCTAGG 1 cut(s) 980
XmiI GTMKAC 1 cut(s) 1059
XmnI GAANNNNTTC 1 cut(s) 1190
XspI CTAG 6 cut(s) 746, 887, 911, 981, 1080, 1316
Zsp2I ATGCAT 3 cut(s) 634, 921, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.