MD15G1081200.v1.1

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005988
Physical Location & Seq
Forward (+)
5569956 .. 5572986
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1081200.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGTATAAATATATAAACTCTTTCCTGCATTTGTATATATGGTTTGAATTGAGACATCGACATAACGCGGCTCATTTCCTCGCTTCCAAGCCAATTATTGTCATGGCTTCTAAGGAACATGTCTGTCACAATTATGTTTTCCTCAAGCCAGAAGAAGCAAGCTTCATCGAGCTCATTCGCCTCCTGTTTTCCTCCGACGTAGAAAAAAGAAGCTTCATCAACTGCTCGGGAGACGAAGGACGTGAACGACGAAAATTTGAACGCCGATGGGTAATCTTCGTCTCTATTGTGGTGCAATGGATGTTCCTTTTCTTCAGAACGCCCATGGCTCGAACTGGGGATACTGTGAACAAGTTGCTAAATCTTGTAGCAACCAATGGCGGATTGTTCGCGCTCTTCAAAATGTGGCTTACAGGAAAGGTGGCAGAGGCAGATTCGTTATCAACCAGATCAGTGCTGGGAAATCTTGATAGGCGAGTTGAATTGGACGACAACATCAAACCAGGCAATACAAGATACGAAGGAGCTCTGTCCATGATGGCGGCTAAAATGTCATACGAAAACCAAGTCCTCATTAAAACAATCGTCACGGGTCACTGGAAGATGAAATTCATAGAGTTTTACAACTTTTGGAATGGTTATCAGAGACGAGATTCAACGCAAGCCTTCCTGTTCCAAGACAAAACATCTGACCCCAACTTGATTGTGGTTGCATTTAGAGGCACCGACCCCTTTGATGCCGACGCATGGAGGACGGATTTCGACATCTCATGGTGCGAGTTTGCAGGACTCGGCAAGACCCACAGCGGCTTCATGAAAGCTCTTGGCATGCAACCAAACAGAAGCTGGCCCCTTGAACAAGAAACCCAACAAGTCAACGCCGCTGAACAACATCCTCATCAGTTTGCTTACTACAAAATCAGACAAATTCTGATGGATTTAATGCAAGAAAACAAGAATGCCAAGTTCATACTGACAGGCCACAGCTTGGGCGGGGCATTGGCCGTGCTGTTTGCCGCGGTGCTTGCAATGCACGACTACGAGGAGAAATATGCATGGTTGATGGGGAGGTTGGAAGGGGTGTACACTTTTGGACAGCCAAGGGTGGGGGATGAGAACTTTGGGGGGTTTATGAGGGAGAAGCTGAAAAATTATGGTGTGAAGTATATGAGGTATGTTTATTCCAATGATATGGTTCCCAGGATACCTTATGATGATAAAACTCTCTTGTTTAAGCACTTTGGTCCAAGCCTATATTTCAGCAGCTGCTATAAGGGGAAGGTTTTGGAGGAGGAACCCAACAAGAACTACTTCAATGTGTTCTGGTTCATACCAAAGTGCTTAAATGCATTGTGGGAGCTGATTAGGAGCTTCATACTGCCGTTAATAAGGGGTGCGGAATACAAGGAAAGCTGGTTTATGAGGATGCTTAGGGTAGTTGGTTTAGTAATTCCTGGATTGGCAGCTCATGCTCCTCAAGATTATGTCAATATTACTCGATTGGGATCCCTTCCTTCACTTGCTCACGTCCAAGAACGTATTCCTCTAGTAAATAATTCTAAAGTCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

524

Amino Acids

60.87

Weight (kDa)

9.1

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 236 - 405 1.5e-31 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 722
AccB7I CCANNNNNTGG 1 cut(s) 988
AccII CGCG 3 cut(s) 68, 392, 1019
AciI CCGC 9 cut(s) 68, 381, 542, 807, 882, 993, 1017, 1019, 1397
AclWI GGATC 2 cut(s) 1500, 1513
AcoI YGGCCR 1 cut(s) 1002
AcsI RAATTY 3 cut(s) 254, 608, 927
AcuI CTGAAG 1 cut(s) 298
AfaI GTAC 1 cut(s) 1085
AfiI CCNNNNNNNGG 3 cut(s) 747, 988, 1106
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 7 cut(s) 47, 260, 400, 482, 657, 857, 1315
AjiI CACGTC 2 cut(s) 242, 1528
AjnI CCWGG 3 cut(s) 502, 1199, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 1524, 1556
Alw21I GWGCWC 2 cut(s) 174, 529
Alw26I GTCTC 4 cut(s) 46, 225, 286, 640
AlwI GGATC 2 cut(s) 1500, 1513
AlwNI CAGNNNCTG 2 cut(s) 846, 1266
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 3 cut(s) 848, 979, 1002
ApeKI GCWGC 3 cut(s) 1263, 1266, 1463
ApoI RAATTY 3 cut(s) 254, 608, 927
ArsI GACNNNNNNTTYG 2 cut(s) 552, 584
Asp700I GAANNNNTTC 2 cut(s) 1310, 1539
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 2 cut(s) 849, 1244
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 1244
BamHI GGATCC 1 cut(s) 1505
BanI GGYRCC 1 cut(s) 722
BanII GRGCYC 2 cut(s) 174, 529
Bbv12I GWGCWC 2 cut(s) 174, 529
BbvI GCAGC 3 cut(s) 1253, 1275, 1475
BccI CCATC 4 cut(s) 261, 532, 928, 1057
BceAI ACGGC 2 cut(s) 989, 1366
BciT130I CCWGG 3 cut(s) 504, 1201, 1455
BciVI GTATCC 2 cut(s) 334, 1197
BcoDI GTCTC 4 cut(s) 46, 225, 286, 640
BfaI CTAG 1 cut(s) 1547
BfuI GTATCC 2 cut(s) 334, 1197
BisI GCNGC 8 cut(s) 69, 543, 808, 882, 1017, 1264, 1267, 1464
BlsI GCNGC 8 cut(s) 70, 544, 809, 883, 1018, 1265, 1268, 1465
Bme1390I CCNGG 3 cut(s) 504, 1201, 1455
Bme18I GGWCC 1 cut(s) 1244
BmeT110I CYCGRG 1 cut(s) 226
BmgBI CACGTC 2 cut(s) 242, 1528
BmgT120I GGNCC 2 cut(s) 849, 1244
BmiI GGNNCC 5 cut(s) 724, 851, 1197, 1296, 1507
BmrFI CCNGG 3 cut(s) 504, 1201, 1455
BmrI ACTGGG 1 cut(s) 345
BmsI GCATC 2 cut(s) 727, 1416
BmuI ACTGGG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 1430
BpuEI CTTGAG 2 cut(s) 128, 1461
BsaBI GATNNNNATC 1 cut(s) 1504
BsaJI CCNNGG 4 cut(s) 324, 1017, 1100, 1199
Bsc4I CCNNNNNNNGG 3 cut(s) 747, 988, 1106
Bse1I ACTGG 2 cut(s) 340, 602
Bse3DI GCAATG 2 cut(s) 302, 1035
Bse8I GATNNNNATC 1 cut(s) 1504
BseBI CCWGG 3 cut(s) 504, 1201, 1455
BseDI CCNNGG 4 cut(s) 324, 1017, 1100, 1199
BseGI GGATG 4 cut(s) 306, 892, 1117, 1431
BseJI GATNNNNATC 1 cut(s) 1504
BseLI CCNNNNNNNGG 3 cut(s) 747, 988, 1106
BseMI GCAATG 2 cut(s) 302, 1035
BseNI ACTGG 2 cut(s) 340, 602
BseRI GAGGAG 3 cut(s) 1058, 1304, 1464
BseXI GCAGC 3 cut(s) 1253, 1275, 1475
BseYI CCCAGC 1 cut(s) 457
Bsh1236I CGCG 3 cut(s) 68, 392, 1019
BshFI GGCC 3 cut(s) 850, 981, 1004
BshNI GGYRCC 1 cut(s) 722
BsiHKAI GWGCWC 2 cut(s) 174, 529
BsiHKCI CYCGRG 1 cut(s) 226
BslI CCNNNNNNNGG 3 cut(s) 747, 988, 1106
BsmAI GTCTC 4 cut(s) 46, 225, 286, 640
BsmBI CGTCTC 3 cut(s) 225, 286, 640
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 3 cut(s) 850, 981, 1004
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 174, 529
Bsp1407I TGTACA 1 cut(s) 1083
Bsp143I GATC 2 cut(s) 449, 1505
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 9 cut(s) 68, 381, 542, 807, 882, 993, 1017, 1019, 1397
BspANI GGCC 3 cut(s) 850, 981, 1004
BspFNI CGCG 3 cut(s) 68, 392, 1019
BspHI TCATGA 1 cut(s) 813
BspLI GGNNCC 5 cut(s) 724, 851, 1197, 1296, 1507
BspPI GGATC 2 cut(s) 1500, 1513
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 722
BsrDI GCAATG 2 cut(s) 302, 1035
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 2 cut(s) 340, 602
BssECI CCNNGG 4 cut(s) 324, 1017, 1100, 1199
BssMI GATC 2 cut(s) 449, 1505
BssT1I CCWWGG 2 cut(s) 324, 1100
Bst2UI CCWGG 3 cut(s) 504, 1201, 1455
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 1469
BstAUI TGTACA 1 cut(s) 1083
BstC8I GCNNGC 5 cut(s) 160, 663, 830, 848, 1026
BstDEI CTNAG 2 cut(s) 111, 1430
BstDSI CCRYGG 2 cut(s) 324, 1017
BstF5I GGATG 4 cut(s) 306, 892, 1117, 1431
BstFNI CGCG 3 cut(s) 68, 392, 1019
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 2 cut(s) 452, 1508
BstMAI GTCTC 4 cut(s) 46, 225, 286, 640
BstMBI GATC 2 cut(s) 449, 1505
BstMWI GCNNNNNNNGC 3 cut(s) 1025, 1030, 1469
BstNI CCWGG 3 cut(s) 504, 1201, 1455
BstNSI RCATGY 2 cut(s) 122, 832
BstSCI CCNGG 3 cut(s) 502, 1199, 1453
BstUI CGCG 3 cut(s) 68, 392, 1019
BstV1I GCAGC 3 cut(s) 1253, 1275, 1475
BstX2I RGATCY 1 cut(s) 1505
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 1 cut(s) 1505
BsuI GTATCC 2 cut(s) 334, 1197
BsuRI GGCC 3 cut(s) 850, 981, 1004
BtgI CCRYGG 2 cut(s) 324, 1017
BtrI CACGTC 2 cut(s) 242, 1528
BtsCI GGATG 4 cut(s) 306, 892, 1117, 1431
BtsIMutI CAGTG 2 cut(s) 459, 595
Cac8I GCNNGC 5 cut(s) 160, 663, 830, 848, 1026
CaiI CAGNNNCTG 2 cut(s) 846, 1266
CciI TCATGA 1 cut(s) 813
CfoI GCGC 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 849, 1244
Cfr42I CCGCGG 1 cut(s) 1020
CseI GACGC 1 cut(s) 752
Csp6I GTAC 1 cut(s) 1084
CviQI GTAC 1 cut(s) 1084
DdeI CTNAG 2 cut(s) 111, 1430
DpnI GATC 2 cut(s) 451, 1507
DpnII GATC 2 cut(s) 449, 1505
EaeI YGGCCR 1 cut(s) 1002
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
EciI GGCGGA 1 cut(s) 396
Ecl136II GAGCTC 2 cut(s) 172, 527
Eco130I CCWWGG 2 cut(s) 324, 1100
Eco24I GRGCYC 2 cut(s) 174, 529
Eco47I GGWCC 1 cut(s) 1244
Eco53kI GAGCTC 2 cut(s) 172, 527
Eco57I CTGAAG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 2 cut(s) 172, 527
EcoRII CCWGG 3 cut(s) 502, 1199, 1453
EcoT14I CCWWGG 2 cut(s) 324, 1100
EcoT22I ATGCAT 2 cut(s) 1057, 1351
EcoT38I GRGCYC 2 cut(s) 174, 529
ErhI CCWWGG 2 cut(s) 324, 1100
Esp3I CGTCTC 3 cut(s) 225, 286, 640
FalI AAGNNNNNCTT 2 cut(s) 1295, 1327
FauI CCCGC 1 cut(s) 986
Fnu4HI GCNGC 8 cut(s) 69, 543, 808, 882, 1017, 1264, 1267, 1464
FokI GGATG 4 cut(s) 313, 879, 1124, 1438
FriOI GRGCYC 2 cut(s) 174, 529
Fsp4HI GCNGC 8 cut(s) 69, 543, 808, 882, 1017, 1264, 1267, 1464
FspBI CTAG 1 cut(s) 1547
GlaI GCGC 1 cut(s) 393
GluI GCNGC 8 cut(s) 69, 543, 808, 882, 1017, 1264, 1267, 1464
GsaI CCCAGC 1 cut(s) 461
HaeIII GGCC 3 cut(s) 850, 981, 1004
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 394
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HindIII AAGCTT 2 cut(s) 160, 211
HinfI GANTC 3 cut(s) 434, 653, 789
Hpy166II GTNNAC 5 cut(s) 245, 349, 877, 1084, 1086
Hpy188I TCNGA 6 cut(s) 196, 317, 645, 691, 923, 933
Hpy188III TCNNGA 4 cut(s) 228, 467, 814, 1478
Hpy8I GTNNAC 5 cut(s) 245, 349, 877, 1084, 1086
Hpy99I CGWCG 3 cut(s) 200, 252, 746
HpyAV CCTTC 7 cut(s) 230, 515, 676, 1070, 1273, 1520, 1524
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 4 cut(s) 198, 241, 1527, 1537
HpyF10VI GCNNNNNNNGC 3 cut(s) 1025, 1030, 1469
HpyF3I CTNAG 2 cut(s) 111, 1430
HpySE526I ACGT 4 cut(s) 198, 241, 1527, 1537
HspAI GCGC 1 cut(s) 392
KspI CCGCGG 1 cut(s) 1020
Kzo9I GATC 2 cut(s) 449, 1505
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 4 cut(s) 524, 1357, 1368, 1477
Lsp1109I GCAGC 3 cut(s) 1253, 1275, 1475
LweI GCATC 2 cut(s) 727, 1416
MaeI CTAG 1 cut(s) 1547
MaeII ACGT 4 cut(s) 198, 241, 1527, 1537
MaeIII GTNAC 3 cut(s) 125, 586, 593
MalI GATC 2 cut(s) 451, 1507
MboI GATC 2 cut(s) 449, 1505
MboII GAAGA 5 cut(s) 164, 268, 304, 388, 613
MflI RGATCY 1 cut(s) 1505
MhlI GDGCHC 2 cut(s) 174, 529
MlyI GAGTC 1 cut(s) 783
MmeI TCCRAC 2 cut(s) 219, 1053
Mph1103I ATGCAT 2 cut(s) 1057, 1351
MroXI GAANNNNTTC 2 cut(s) 1310, 1539
MseI TTAA 6 cut(s) 576, 941, 1233, 1343, 1385, 1570
MslI CAYNNNNRTG 1 cut(s) 132
MspA1I CMGCKG 4 cut(s) 807, 884, 1019, 1266
MspR9I CCNGG 3 cut(s) 504, 1201, 1455
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 3 cut(s) 504, 1201, 1455
MvnI CGCG 3 cut(s) 68, 392, 1019
MwoI GCNNNNNNNGC 3 cut(s) 1025, 1030, 1469
NcoI CCATGG 1 cut(s) 324
NdeII GATC 2 cut(s) 449, 1505
NlaIV GGNNCC 5 cut(s) 724, 851, 1197, 1296, 1507
NmeAIII GCCGAG 1 cut(s) 771
NmuCI GTSAC 3 cut(s) 125, 586, 593
NsiI ATGCAT 2 cut(s) 1057, 1351
NspI RCATGY 2 cut(s) 122, 832
PaeI GCATGC 1 cut(s) 832
PagI TCATGA 1 cut(s) 813
PciI ACATGT 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PctI GAATGC 1 cut(s) 964
PdmI GAANNNNTTC 2 cut(s) 1310, 1539
PfeI GAWTC 2 cut(s) 434, 653
PflMI CCANNNNNTGG 1 cut(s) 988
PfoI TCCNGGA 1 cut(s) 1453
PkrI GCNGC 8 cut(s) 70, 544, 809, 883, 1018, 1265, 1268, 1465
PleI GAGTC 1 cut(s) 783
PpsI GAGTC 1 cut(s) 783
PscI ACATGT 1 cut(s) 118
Psp124BI GAGCTC 2 cut(s) 174, 529
Psp6I CCWGG 3 cut(s) 502, 1199, 1453
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 3 cut(s) 502, 1199, 1453
PspN4I GGNNCC 5 cut(s) 724, 851, 1197, 1296, 1507
PspPI GGNCC 2 cut(s) 849, 1244
PsrI GAACNNNNNNTAC 2 cut(s) 325, 357
PstNI CAGNNNCTG 2 cut(s) 846, 1266
PsuI RGATCY 1 cut(s) 1505
PvuII CAGCTG 1 cut(s) 1266
RsaI GTAC 1 cut(s) 1085
RsaNI GTAC 1 cut(s) 1084
RseI CAYNNNNRTG 1 cut(s) 132
SacI GAGCTC 2 cut(s) 174, 529
SacII CCGCGG 1 cut(s) 1020
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 6 cut(s) 576, 941, 1233, 1343, 1385, 1570
SatI GCNGC 8 cut(s) 69, 543, 808, 882, 1017, 1264, 1267, 1464
Sau3AI GATC 2 cut(s) 449, 1505
Sau96I GGNCC 2 cut(s) 849, 1244
SchI GAGTC 1 cut(s) 783
ScrFI CCNGG 3 cut(s) 504, 1201, 1455
SduI GDGCHC 2 cut(s) 174, 529
SfaNI GCATC 2 cut(s) 727, 1416
Sfr303I CCGCGG 1 cut(s) 1020
SgrBI CCGCGG 1 cut(s) 1020
SinI GGWCC 1 cut(s) 1244
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 2 cut(s) 143, 1476
SmoI CTYRAG 2 cut(s) 143, 1476
SphI GCATGC 1 cut(s) 832
SsiI CCGC 9 cut(s) 68, 381, 542, 807, 882, 993, 1017, 1019, 1397
SspI AATATT 1 cut(s) 1492
SspMI CTAG 1 cut(s) 1547
SstI GAGCTC 2 cut(s) 174, 529
StyD4I CCNGG 3 cut(s) 502, 1199, 1453
StyI CCWWGG 2 cut(s) 324, 1100
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 4 cut(s) 201, 244, 1530, 1540
TaqI TCGA 6 cut(s) 58, 168, 331, 762, 1498, 1566
TatI WGTACW 1 cut(s) 1083
TauI GCSGC 5 cut(s) 71, 545, 810, 884, 1019
TfiI GAWTC 2 cut(s) 434, 653
Tru1I TTAA 6 cut(s) 576, 941, 1233, 1343, 1385, 1570
Tru9I TTAA 6 cut(s) 576, 941, 1233, 1343, 1385, 1570
TscAI CASTG 2 cut(s) 459, 602
TseFI GTSAC 3 cut(s) 125, 586, 593
TseI GCWGC 3 cut(s) 1263, 1266, 1463
Tsp45I GTSAC 3 cut(s) 125, 586, 593
TspDTI ATGAA 9 cut(s) 154, 205, 601, 620, 802, 830, 958, 1318, 1363
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 2 cut(s) 459, 602
Van91I CCANNNNNTGG 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 1244
XapI RAATTY 3 cut(s) 254, 608, 927
XceI RCATGY 2 cut(s) 122, 832
XcmI CCANNNNNNNNNTGG 3 cut(s) 454, 703, 843
XmnI GAANNNNTTC 2 cut(s) 1310, 1539
XspI CTAG 1 cut(s) 1547
Zsp2I ATGCAT 2 cut(s) 1057, 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.