RLG00000010670

triglyceride lipase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005988
Physical Location & Seq
Forward (+)
3481548 .. 3484843
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010670

Sequence Viewer

Length: 1434 bp
ATGGCTTCTAAGGAACATGTATGTGACAATTACGTGTTTTTGAAGCCAAAAGAAGTAACTCTTATCGAGCTTATTCGCTTTCTGTTTTCCTTCGATTTAGAGAACAGAAGATTCATCAATTCCTCGGAAGAGGAAGGGAAGAAGGGAGCGAATTTAGAACGGAGGTGGATAATCTTCATCTCTGTTGTAGTGCAATGGGTTTTCCTCTTTTTCTGTGTTCCTATGGCGAGAATAGGGGATGTGCTCCAGAAATGGCTCAACCTTGTCTCGACCAATGGCGGACCAGTCATGCTCTTCTTAAACTGGCTAACAGGAGAGGTGGTCGAACCGGATTCATTATTATCGACATCAGTGTTGGGGAATCTTGACAGGAGAATTGAATTAGACAAGAGCATCAAACCTGAGGATACACGATACAAAGCGGCTCTCTCCATCATGGCTGCAAAGCTGTCATATGAGAACAAAGATCTCGTTCAATCCATCGTTAATCATCACTGGAAGATGAAATTCGTGGGATTTTACAACTTTCGTAATGGTTACCAAGGACGAGATTCAACACAGGCCTTCCTGTTCAGAGACACATTGTCTGACCCTAGCTTGATTGTGGTGGCATTTAGAGGCACTGCTCCATTTGATGCGGATGCATGGAGGACAGATTTCGACATCTCATGGTGCGAGTTCAACAAAGTCGGTAAGACGCACGGCGGCTTCATGAAAGCTCTTGGCCTGCAACCAAACAATCGTTGGCCACTTCAACTAGAAACTGATCAACTACAAGGCAGTCACCAACATCCTCAGTTTGCTTACTACACAATCAGACAAATGCTGAAAGATTTACTGCAAGAGGATGAGAATGCCAAGTTTATATTGACGGGGCACAGCTTGGGCGGGGCGTTGGCTGTTTTGTTTGCCGGGGTGCTAGCAATGCATGACTGTGAGTATGCCTGGTTGCTGGAGAGGTTGGAGGGGGTTTACACGTTCGGACAGCCTAGGGTTGGAGATGGGAAGTTTGGGGTGTTTATGAAGGAAAAGTTTAGGAAATATGATGTGAGATATATGAGGTATGTTTACAGCAATGACCTGGTGCCTAGAATACCTTATGATGATAAAGCCCTCTTGTTCAAGCACTTTGGGCCAAGTCTTTACTACAACAGCTGCTATAGAGGAAAGGTTTTGAAGGAGGAACCCAACAAAAACTACTTTAGTTTGCTATGGTTCATACCTAGGAGGCTGAATGCAGCGTGGGAGCTGTTAAGGAGCTTCATACTCCCTTTGGTAAGGGGTTCAGACTACAAAGAGGGGTGGTTTATGAGATTTTGGAGGCTGGTGGGAATAGTAATTCCAGGATTGGCAGCCCATGCTCCTCAAGATTACGTCAATCTTACAAGATTGGGAACCCTGCTTATTCCTAAAGAAAGTCCTAAAGTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

478

Amino Acids

55.38

Weight (kDa)

8.91

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 202 - 368 7.1e-31 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1084
AciI CCGC 5 cut(s) 279, 422, 638, 705, 888
AcoI YGGCCR 1 cut(s) 746
AcsI RAATTY 2 cut(s) 151, 506
AfiI CCNNNNNNNGG 1 cut(s) 995
AflIII ACRYGT 3 cut(s) 16, 33, 975
AgsI TTSAA 8 cut(s) 43, 380, 476, 555, 682, 755, 1123, 1177
AhdI GACNNNNNGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 944, 1080, 1342
AluBI AGCT 8 cut(s) 70, 448, 597, 719, 882, 1155, 1249, 1260
AluI AGCT 8 cut(s) 70, 448, 597, 719, 882, 1155, 1249, 1260
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 271, 570
AoxI GGCC 4 cut(s) 561, 724, 746, 1133
ApeKI GCWGC 4 cut(s) 440, 1155, 1238, 1352
ApoI RAATTY 2 cut(s) 151, 506
AspA2I CCTAGG 2 cut(s) 989, 1223
AspS9I GGNCC 2 cut(s) 281, 1133
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 1 cut(s) 776
AsuNHI GCTAGC 1 cut(s) 919
AvaII GGWCC 1 cut(s) 281
AvrII CCTAGG 2 cut(s) 989, 1223
AxyI CCTNAGG 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 879
BalI TGGCCA 1 cut(s) 748
BanI GGYRCC 1 cut(s) 1084
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 4 cut(s) 427, 1142, 1250, 1364
BccI CCATC 3 cut(s) 440, 488, 995
BceAI ACGGC 1 cut(s) 718
BciT130I CCWGG 3 cut(s) 946, 1082, 1344
BciVI GTATCC 1 cut(s) 400
BclI TGATCA 1 cut(s) 766
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 271, 570
BfaI CTAG 6 cut(s) 594, 758, 920, 990, 1089, 1224
BfmI CTRYAG 1 cut(s) 1159
BfuI GTATCC 1 cut(s) 400
BglII AGATCT 1 cut(s) 466
BisI GCNGC 6 cut(s) 423, 441, 706, 1156, 1239, 1353
BlnI CCTAGG 2 cut(s) 989, 1223
BlsI GCNGC 6 cut(s) 424, 442, 707, 1157, 1240, 1354
Bme1390I CCNGG 4 cut(s) 913, 946, 1082, 1344
Bme18I GGWCC 1 cut(s) 281
BmeRI GACNNNNNGTC 1 cut(s) 583
BmgT120I GGNCC 2 cut(s) 281, 1133
BmiI GGNNCC 3 cut(s) 1086, 1185, 1396
BmrFI CCNGG 4 cut(s) 913, 946, 1082, 1344
BmsI GCATC 3 cut(s) 402, 625, 631
BmtI GCTAGC 1 cut(s) 923
BpmI CTGGAG 2 cut(s) 230, 974
BpuEI CTTGAG 1 cut(s) 1350
BpuMI CCSGG 1 cut(s) 913
BsaAI YACGTR 1 cut(s) 34
BsaJI CCNNGG 5 cut(s) 123, 541, 912, 989, 1223
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 995
Bse1I ACTGG 3 cut(s) 284, 308, 500
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 3 cut(s) 200, 930, 1081
BseBI CCWGG 3 cut(s) 946, 1082, 1344
BseDI CCNNGG 5 cut(s) 123, 541, 912, 989, 1223
BseGI GGATG 4 cut(s) 244, 646, 790, 853
BseLI CCNNNNNNNGG 1 cut(s) 995
BseMI GCAATG 3 cut(s) 200, 930, 1081
BseMII CTCAG 2 cut(s) 393, 809
BseNI ACTGG 3 cut(s) 284, 308, 500
BseRI GAGGAG 1 cut(s) 1353
BseSI GKGCMC 1 cut(s) 879
BseXI GCAGC 4 cut(s) 427, 1142, 1250, 1364
BshFI GGCC 4 cut(s) 563, 726, 748, 1135
BshNI GGYRCC 1 cut(s) 1084
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 2 cut(s) 329, 912
BslI CCNNNNNNNGG 1 cut(s) 995
BsmAI GTCTC 2 cut(s) 271, 570
BsmI GAATGC 2 cut(s) 859, 1240
BsnI GGCC 4 cut(s) 563, 726, 748, 1135
Bsp1286I GDGCHC 2 cut(s) 246, 879
Bsp143I GATC 2 cut(s) 466, 766
BspACI CCGC 5 cut(s) 279, 422, 638, 705, 888
BspANI GGCC 4 cut(s) 563, 726, 748, 1135
BspCNI CTCAG 2 cut(s) 394, 808
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 3 cut(s) 1086, 1185, 1396
BspOI GCTAGC 1 cut(s) 923
BspQI GCTCTTC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 1084
BsrDI GCAATG 3 cut(s) 200, 930, 1081
BsrI ACTGG 3 cut(s) 284, 308, 500
BssECI CCNNGG 5 cut(s) 123, 541, 912, 989, 1223
BssMI GATC 2 cut(s) 466, 766
BssT1I CCWWGG 3 cut(s) 541, 989, 1223
Bst2UI CCWGG 3 cut(s) 946, 1082, 1344
Bst4CI ACNGT 1 cut(s) 935
Bst6I CTCTTC 2 cut(s) 123, 299
BstAPI GCANNNNNTGC 1 cut(s) 1358
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 728, 921
BstDEI CTNAG 3 cut(s) 9, 402, 795
BstEII GGTNACC 1 cut(s) 536
BstF5I GGATG 4 cut(s) 244, 646, 790, 853
BstKTI GATC 2 cut(s) 469, 769
BstMAI GTCTC 2 cut(s) 271, 570
BstMBI GATC 2 cut(s) 466, 766
BstMWI GCNNNNNNNGC 3 cut(s) 925, 1132, 1358
BstNI CCWGG 3 cut(s) 946, 1082, 1344
BstNSI RCATGY 1 cut(s) 20
BstPI GGTNACC 1 cut(s) 536
BstSCI CCNGG 4 cut(s) 911, 944, 1080, 1342
BstSFI CTRYAG 1 cut(s) 1159
BstSLI GKGCMC 1 cut(s) 879
BstV1I GCAGC 4 cut(s) 427, 1142, 1250, 1364
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
Bsu36I CCTNAGG 1 cut(s) 402
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 4 cut(s) 563, 726, 748, 1135
BtsCI GGATG 4 cut(s) 244, 646, 790, 853
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 3 cut(s) 357, 493, 621
Cac8I GCNNGC 2 cut(s) 728, 921
CciI TCATGA 1 cut(s) 711
Cfr13I GGNCC 2 cut(s) 281, 1133
CseI GACGC 1 cut(s) 706
CsiI ACCWGGT 1 cut(s) 1080
CviAII CATG 8 cut(s) 17, 289, 436, 645, 669, 712, 929, 1358
DdeI CTNAG 3 cut(s) 9, 402, 795
DpnI GATC 2 cut(s) 468, 768
DpnII GATC 2 cut(s) 466, 766
DriI GACNNNNNGTC 1 cut(s) 583
EaeI YGGCCR 1 cut(s) 746
Eam1104I CTCTTC 2 cut(s) 123, 299
Eam1105I GACNNNNNGTC 1 cut(s) 583
EarI CTCTTC 2 cut(s) 123, 299
EciI GGCGGA 1 cut(s) 294
Eco130I CCWWGG 3 cut(s) 541, 989, 1223
Eco147I AGGCCT 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 281
Eco81I CCTNAGG 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 536
EcoRII CCWGG 3 cut(s) 944, 1080, 1342
EcoT14I CCWWGG 3 cut(s) 541, 989, 1223
EcoT22I ATGCAT 2 cut(s) 646, 930
ErhI CCWWGG 3 cut(s) 541, 989, 1223
FaeI CATG 8 cut(s) 20, 292, 439, 648, 672, 715, 932, 1361
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FatI CATG 8 cut(s) 16, 288, 435, 644, 668, 711, 928, 1357
FauI CCCGC 1 cut(s) 881
FauNDI CATATG 1 cut(s) 454
FbaI TGATCA 1 cut(s) 766
Fnu4HI GCNGC 6 cut(s) 423, 441, 706, 1156, 1239, 1353
FokI GGATG 4 cut(s) 251, 653, 777, 860
Fsp4HI GCNGC 6 cut(s) 423, 441, 706, 1156, 1239, 1353
FspBI CTAG 6 cut(s) 594, 758, 920, 990, 1089, 1224
GluI GCNGC 6 cut(s) 423, 441, 706, 1156, 1239, 1353
GsuI CTGGAG 2 cut(s) 230, 974
HaeIII GGCC 4 cut(s) 563, 726, 748, 1135
HapII CCGG 2 cut(s) 329, 912
HgaI GACGC 1 cut(s) 706
Hin1II CATG 8 cut(s) 20, 292, 439, 648, 672, 715, 932, 1361
HinfI GANTC 4 cut(s) 111, 332, 361, 551
HpaII CCGG 2 cut(s) 329, 912
HphI GGTGA 1 cut(s) 776
Hpy166II GTNNAC 2 cut(s) 973, 1069
Hpy188I TCNGA 6 cut(s) 127, 575, 589, 818, 983, 1288
Hpy188III TCNNGA 5 cut(s) 247, 268, 365, 712, 1367
Hpy8I GTNNAC 2 cut(s) 973, 1069
HpyAV CCTTC 6 cut(s) 100, 128, 136, 574, 1018, 1171
HpyCH4III ACNGT 1 cut(s) 935
HpyCH4IV ACGT 3 cut(s) 33, 977, 1374
HpyCH4V TGCA 7 cut(s) 193, 443, 644, 730, 841, 928, 1238
HpyF10VI GCNNNNNNNGC 3 cut(s) 925, 1132, 1358
HpyF3I CTNAG 3 cut(s) 9, 402, 795
HpySE526I ACGT 3 cut(s) 33, 977, 1374
Hsp92II CATG 8 cut(s) 20, 292, 439, 648, 672, 715, 932, 1361
Ksp22I TGATCA 1 cut(s) 766
Kzo9I GATC 2 cut(s) 466, 766
LguI GCTCTTC 1 cut(s) 299
LmnI GCTCC 6 cut(s) 146, 249, 631, 1246, 1257, 1366
Lsp1109I GCAGC 4 cut(s) 427, 1142, 1250, 1364
LweI GCATC 3 cut(s) 402, 625, 631
MabI ACCWGGT 1 cut(s) 1080
MaeI CTAG 6 cut(s) 594, 758, 920, 990, 1089, 1224
MaeII ACGT 3 cut(s) 33, 977, 1374
MaeIII GTNAC 4 cut(s) 23, 55, 536, 782
MalI GATC 2 cut(s) 468, 768
MboI GATC 2 cut(s) 466, 766
MboII GAAGA 6 cut(s) 120, 140, 151, 166, 286, 511
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 246, 879
MlsI TGGCCA 1 cut(s) 748
MluCI AATT 7 cut(s) 28, 118, 151, 375, 380, 506, 1338
MluNI TGGCCA 1 cut(s) 748
MmeI TCCRAC 2 cut(s) 942, 976
Mox20I TGGCCA 1 cut(s) 748
Mph1103I ATGCAT 2 cut(s) 646, 930
MscI TGGCCA 1 cut(s) 748
MseI TTAA 3 cut(s) 299, 486, 1253
MslI CAYNNNNRTG 2 cut(s) 21, 933
Msp20I TGGCCA 1 cut(s) 748
MspA1I CMGCKG 1 cut(s) 1155
MspI CCGG 2 cut(s) 329, 912
MspR9I CCNGG 4 cut(s) 913, 946, 1082, 1344
Mva1269I GAATGC 2 cut(s) 859, 1240
MvaI CCWGG 3 cut(s) 946, 1082, 1344
MwoI GCNNNNNNNGC 3 cut(s) 925, 1132, 1358
NciI CCSGG 1 cut(s) 913
NdeI CATATG 1 cut(s) 454
NdeII GATC 2 cut(s) 466, 766
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 8 cut(s) 20, 292, 439, 648, 672, 715, 932, 1361
NlaIV GGNNCC 3 cut(s) 1086, 1185, 1396
NmuCI GTSAC 2 cut(s) 23, 782
NsiI ATGCAT 2 cut(s) 646, 930
NspI RCATGY 1 cut(s) 20
PagI TCATGA 1 cut(s) 711
PceI AGGCCT 1 cut(s) 563
PciI ACATGT 1 cut(s) 16
PciSI GCTCTTC 1 cut(s) 299
PctI GAATGC 2 cut(s) 859, 1240
PfeI GAWTC 4 cut(s) 111, 332, 361, 551
PfoI TCCNGGA 1 cut(s) 1342
PkrI GCNGC 6 cut(s) 424, 442, 707, 1157, 1240, 1354
Ppu21I YACGTR 1 cut(s) 34
PscI ACATGT 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 944, 1080, 1342
PspEI GGTNACC 1 cut(s) 536
PspGI CCWGG 3 cut(s) 944, 1080, 1342
PspN4I GGNNCC 3 cut(s) 1086, 1185, 1396
PspPI GGNCC 2 cut(s) 281, 1133
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 1155
RseI CAYNNNNRTG 2 cut(s) 21, 933
SapI GCTCTTC 1 cut(s) 299
SaqAI TTAA 3 cut(s) 299, 486, 1253
SatI GCNGC 6 cut(s) 423, 441, 706, 1156, 1239, 1353
Sau3AI GATC 2 cut(s) 466, 766
Sau96I GGNCC 2 cut(s) 281, 1133
ScrFI CCNGG 4 cut(s) 913, 946, 1082, 1344
SduI GDGCHC 2 cut(s) 246, 879
SexAI ACCWGGT 1 cut(s) 1080
SfaNI GCATC 3 cut(s) 402, 625, 631
SfcI CTRYAG 1 cut(s) 1159
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 2 cut(s) 21, 933
SmlI CTYRAG 1 cut(s) 1365
SmoI CTYRAG 1 cut(s) 1365
Sse9I AATT 7 cut(s) 28, 118, 151, 375, 380, 506, 1338
SseBI AGGCCT 1 cut(s) 563
SsiI CCGC 5 cut(s) 279, 422, 638, 705, 888
SspMI CTAG 6 cut(s) 594, 758, 920, 990, 1089, 1224
StuI AGGCCT 1 cut(s) 563
StyD4I CCNGG 4 cut(s) 911, 944, 1080, 1342
StyI CCWWGG 3 cut(s) 541, 989, 1223
TaaI ACNGT 1 cut(s) 935
TaiI ACGT 3 cut(s) 36, 980, 1377
TaqI TCGA 7 cut(s) 66, 93, 269, 324, 344, 660, 1428
TasI AATT 7 cut(s) 28, 118, 151, 375, 380, 506, 1338
TauI GCSGC 2 cut(s) 425, 708
TfiI GAWTC 4 cut(s) 111, 332, 361, 551
Tru1I TTAA 3 cut(s) 299, 486, 1253
Tru9I TTAA 3 cut(s) 299, 486, 1253
TscAI CASTG 3 cut(s) 357, 500, 628
TseFI GTSAC 2 cut(s) 23, 782
TseI GCWGC 4 cut(s) 440, 1155, 1238, 1352
Tsp45I GTSAC 2 cut(s) 23, 782
TspDTI ATGAA 9 cut(s) 103, 166, 324, 518, 700, 728, 1037, 1207, 1252
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 3 cut(s) 357, 500, 628
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 2 cut(s) 151, 506
XceI RCATGY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 741
XmaJI CCTAGG 2 cut(s) 989, 1223
XspI CTAG 6 cut(s) 594, 758, 920, 990, 1089, 1224
Zsp2I ATGCAT 2 cut(s) 646, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.