AT1G60230

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015306
Physical Location & Seq
Reverse (-)
22212084 .. 22214431
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60230.1

Sequence Viewer

Length: 1377 bp
ATGTCGACGACAATGAGGCGTCTTGGAATCATCACTCTCACCAATTCCGCCGCCGTCGTCACTCCGTCGCTACTCTCTTCAACGGCAGCTTTCCCCGGAACCCTAACTTTACCCTCTCCCCTCCTCAGCGACCGTCTTACCAATTCAATTCTCTTTCACGAATCCAGAAGCCTCTCCTTCTCTTGCTTCTCTTCTTCTTCTTCTTCTTCAGCAGCTTATCTTCCTTCACTAGAAGAGTACCCATCAGCTAAAGGTTCTGTGAAGAGAGATGATAACCAGAAGGTGGTCTTGAAGGGTATGACTTATGCTTCGCTCCAAGAATGGGTTCAATCTCATGGGTTTAGACCAGGACAGGCTTTAATGTTGTGGAAGAGATTGTACAAGGACAATATATGGGCAAATAATGTTGATGAACTTGAAGGTTTGAACAAAGATCTCAAGAGAATGATTAGTGAACATGCTGAGTTTGGGGCATTATCTTTTAAGGATATTCGTTCGGCTTCAGATGGAACTAGGAAGATTCTGTTCACGTTGGATGATGGACTTGTGATTGAGACTGTTGTTATACCTTGTGATCGAGGCAGGACAACAGTTTGTGTTTCGAGCCAAGTTGGTTGTGCCATGAATTGTCAGTTCTGCTACACTGGCAGGATGGGCTTGAAGAGAAATCTCACTACTGCTGAGATAGTTGAGCAGGCTGTTTATGCCAGGCGATTGCTTTCCCATGAAGTTGGGTCAATTACAAATGTCGTCTTTATGGGAATGGGAGAGCCATTTCACAACATTGACAATGTCATCAAAGCTGCAAACATAATGGTAGACGAGAATGGACTTCACTTCAGTCCCCGCAAGGTCACAGTCTCGACTAGTGGCCTTGTTCCTCAGCTGAAGCGTTTTCTCCGTGAGTCAAATTGCGCTTTGGCAGTCAGTCTGAATGCAACAACTGATGAGGTCAGAAACTGGATTATGCCTATAAACAGGAAATACAAGTTATCTTTGCTCCTTGAGACGCTCAGAGAAGGACTCAGTTCAAGGCATAAGTACAAAGTGTTGTTTGAATATGTAATGCTCGCAGGAGTCAATGACAGCATGGATGATGCAAGGAGGCTAGTGGAGCTTGTGCAGGGGATTCCATGCAAGATCAACCTTATTCAATTCAACCCACACAGTGGTTCTCAGTTCATACAGACCGAGGAAGACAAGATGATCAAGTTTCGTAACGTTTTGGCTGAAGGAGGCTGCACTGTTCTCATGCGGTTTAGCCGAGGCAATGATCAAATGGCAGCATGTGGGCAGCTTGGTATGATTGGGGCGGTTCAGGCACCAGTGATGCGGGTGCCCGAGCAGTTCCGCACCGCTTTAAAAGCATCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

458

Amino Acids

50.76

Weight (kDa)

9.01

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 201 - 369 3.4e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1321, 1336
AccB7I CCANNNNNTGG 2 cut(s) 283, 1169
AccI GTMKAC 2 cut(s) 5, 819
AciI CCGC 8 cut(s) 48, 51, 847, 1255, 1313, 1333, 1351, 1356
AclI AACGTT 1 cut(s) 1221
AcuI CTGAAG 5 cut(s) 192, 486, 823, 908, 1251
AcyI GRCGYC 1 cut(s) 19
AdeI CACNNNGTG 1 cut(s) 1169
AfaI GTAC 3 cut(s) 239, 380, 1043
AfiI CCNNNNNNNGG 3 cut(s) 283, 322, 1169
AhlI ACTAGT 1 cut(s) 866
AjnI CCWGG 2 cut(s) 346, 707
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AluBI AGCT 7 cut(s) 89, 215, 248, 803, 886, 1117, 1297
AluI AGCT 7 cut(s) 89, 215, 248, 803, 886, 1117, 1297
Alw26I GTCTC 3 cut(s) 548, 865, 1001
AlwNI CAGNNNCTG 1 cut(s) 960
Ama87I CYCGRG 1 cut(s) 1340
AoxI GGCC 1 cut(s) 871
ApeKI GCWGC 6 cut(s) 86, 212, 803, 1239, 1283, 1294
ArsI GACNNNNNNTTYG 2 cut(s) 292, 324
Asp700I GAANNNNTTC 1 cut(s) 324
AspLEI GCGC 1 cut(s) 917
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 31
AvaI CYCGRG 1 cut(s) 1340
BaeGI GKGCMC 1 cut(s) 1341
BanI GGYRCC 2 cut(s) 1321, 1336
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
BbsI GAAGAC 1 cut(s) 1203
BbvCI CCTCAGC 2 cut(s) 125, 882
BbvI GCAGC 6 cut(s) 98, 224, 790, 1226, 1295, 1306
BccI CCATC 4 cut(s) 250, 500, 533, 646
BceAI ACGGC 2 cut(s) 38, 99
BciT130I CCWGG 2 cut(s) 348, 709
BclI TGATCA 2 cut(s) 1206, 1273
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 548, 865, 1001
BcuI ACTAGT 1 cut(s) 866
BfaI CTAG 4 cut(s) 230, 513, 867, 1109
BglII AGATCT 1 cut(s) 433
BisI GCNGC 7 cut(s) 51, 87, 213, 804, 1240, 1284, 1295
BlsI GCNGC 7 cut(s) 52, 88, 214, 805, 1241, 1285, 1296
Bme1390I CCNGG 3 cut(s) 96, 348, 709
BmeT110I CYCGRG 1 cut(s) 1340
BmiI GGNNCC 3 cut(s) 100, 1323, 1338
BmrFI CCNGG 3 cut(s) 96, 348, 709
BmsI GCATC 2 cut(s) 1087, 1320
BpiI GAAGAC 1 cut(s) 1203
BplI GAGNNNNNCTC 2 cut(s) 1008, 1040
Bpu10I CCTNAGC 2 cut(s) 125, 882
BpuEI CTTGAG 2 cut(s) 422, 1025
BpuMI CCSGG 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 94, 1191, 1264
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 322, 1169
Bse1I ACTGG 3 cut(s) 649, 965, 1325
Bse3DI GCAATG 1 cut(s) 1276
BseBI CCWGG 2 cut(s) 348, 709
BseDI CCNNGG 3 cut(s) 94, 1191, 1264
BseGI GGATG 3 cut(s) 541, 657, 1099
BseLI CCNNNNNNNGG 3 cut(s) 283, 322, 1169
BseMI GCAATG 1 cut(s) 1276
BseMII CTCAG 7 cut(s) 139, 453, 672, 896, 1027, 1039, 1190
BseNI ACTGG 3 cut(s) 649, 965, 1325
BseRI GAGGAG 1 cut(s) 113
BseSI GKGCMC 1 cut(s) 1341
BseXI GCAGC 6 cut(s) 98, 224, 790, 1226, 1295, 1306
BsgI GTGCAG 2 cut(s) 1142, 1225
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 1 cut(s) 873
BshNI GGYRCC 2 cut(s) 1321, 1336
BsiEI CGRYCG 1 cut(s) 133
BsiHKCI CYCGRG 1 cut(s) 1340
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 3 cut(s) 283, 322, 1169
BsmAI GTCTC 3 cut(s) 548, 865, 1001
BsmBI CGTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 828
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 1 cut(s) 873
BsoBI CYCGRG 1 cut(s) 1340
Bsp1286I GDGCHC 1 cut(s) 1341
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 5 cut(s) 433, 574, 1140, 1206, 1273
BspACI CCGC 8 cut(s) 48, 51, 847, 1255, 1313, 1333, 1351, 1356
BspANI GGCC 1 cut(s) 873
BspCNI CTCAG 7 cut(s) 138, 454, 673, 895, 1026, 1038, 1189
BspLI GGNNCC 3 cut(s) 100, 1323, 1338
BspT107I GGYRCC 2 cut(s) 1321, 1336
BsrDI GCAATG 1 cut(s) 1276
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 3 cut(s) 649, 965, 1325
BssECI CCNNGG 3 cut(s) 94, 1191, 1264
BssMI GATC 5 cut(s) 433, 574, 1140, 1206, 1273
BssNI GRCGYC 1 cut(s) 19
Bst2UI CCWGG 2 cut(s) 348, 709
Bst4CI ACNGT 6 cut(s) 134, 559, 592, 859, 1169, 1246
Bst6I CTCTTC 6 cut(s) 82, 196, 228, 257, 365, 656
BstACI GRCGYC 1 cut(s) 19
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 696, 1071
BstDEI CTNAG 7 cut(s) 125, 462, 681, 882, 1013, 1025, 1176
BstF5I GGATG 3 cut(s) 541, 657, 1099
BstHHI GCGC 1 cut(s) 917
BstKTI GATC 5 cut(s) 436, 577, 1143, 1209, 1276
BstMAI GTCTC 3 cut(s) 548, 865, 1001
BstMBI GATC 5 cut(s) 433, 574, 1140, 1206, 1273
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 6 cut(s) 645, 654, 704, 1114, 1319, 1364
BstNI CCWGG 2 cut(s) 348, 709
BstNSI RCATGY 2 cut(s) 461, 1290
BstSCI CCNGG 3 cut(s) 94, 346, 707
BstSLI GKGCMC 1 cut(s) 1341
BstV1I GCAGC 6 cut(s) 98, 224, 790, 1226, 1295, 1306
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 1 cut(s) 873
BtsCI GGATG 3 cut(s) 541, 657, 1099
BtsIMutI CAGTG 4 cut(s) 642, 1174, 1242, 1332
Cac8I GCNNGC 2 cut(s) 696, 1071
CaiI CAGNNNCTG 1 cut(s) 960
CfoI GCGC 1 cut(s) 917
CseI GACGC 2 cut(s) 8, 1018
Csp6I GTAC 3 cut(s) 238, 379, 1042
CviAII CATG 8 cut(s) 335, 458, 622, 725, 1090, 1134, 1252, 1287
CviQI GTAC 3 cut(s) 238, 379, 1042
DdeI CTNAG 7 cut(s) 125, 462, 681, 882, 1013, 1025, 1176
DpnI GATC 5 cut(s) 435, 576, 1142, 1208, 1275
DpnII GATC 5 cut(s) 433, 574, 1140, 1206, 1273
DraI TTTAAA 1 cut(s) 1362
DraIII CACNNNGTG 1 cut(s) 1169
Eam1104I CTCTTC 6 cut(s) 82, 196, 228, 257, 365, 656
EarI CTCTTC 6 cut(s) 82, 196, 228, 257, 365, 656
EciI GGCGGA 1 cut(s) 37
Eco57I CTGAAG 5 cut(s) 192, 486, 823, 908, 1251
Eco88I CYCGRG 1 cut(s) 1340
EcoRII CCWGG 2 cut(s) 346, 707
Esp3I CGTCTC 1 cut(s) 1001
FaeI CATG 8 cut(s) 338, 461, 625, 728, 1093, 1137, 1255, 1290
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 828
FatI CATG 8 cut(s) 334, 457, 621, 724, 1089, 1133, 1251, 1286
FauI CCCGC 2 cut(s) 854, 1326
FbaI TGATCA 2 cut(s) 1206, 1273
FblI GTMKAC 2 cut(s) 5, 819
Fnu4HI GCNGC 7 cut(s) 51, 87, 213, 804, 1240, 1284, 1295
FokI GGATG 3 cut(s) 548, 664, 1106
Fsp4HI GCNGC 7 cut(s) 51, 87, 213, 804, 1240, 1284, 1295
FspBI CTAG 4 cut(s) 230, 513, 867, 1109
GlaI GCGC 1 cut(s) 916
GluI GCNGC 7 cut(s) 51, 87, 213, 804, 1240, 1284, 1295
HaeIII GGCC 1 cut(s) 873
HapII CCGG 1 cut(s) 96
HgaI GACGC 2 cut(s) 8, 1018
HhaI GCGC 1 cut(s) 917
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 8 cut(s) 338, 461, 625, 728, 1093, 1137, 1255, 1290
Hin6I GCGC 1 cut(s) 915
HinP1I GCGC 1 cut(s) 915
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 7 cut(s) 27, 161, 520, 905, 1023, 1077, 1129
HpaII CCGG 1 cut(s) 96
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 4 cut(s) 6, 455, 528, 820
Hpy188I TCNGA 4 cut(s) 505, 933, 956, 1016
Hpy188III TCNNGA 5 cut(s) 158, 165, 289, 439, 862
Hpy8I GTNNAC 4 cut(s) 6, 455, 528, 820
Hpy99I CGWCG 3 cut(s) 10, 59, 70
HpyAV CCTTC 7 cut(s) 187, 234, 274, 286, 413, 1013, 1226
HpyCH4III ACNGT 6 cut(s) 134, 559, 592, 859, 1169, 1246
HpyCH4IV ACGT 2 cut(s) 530, 1221
HpyCH4V TGCA 6 cut(s) 806, 938, 1100, 1123, 1137, 1242
HpyF10VI GCNNNNNNNGC 6 cut(s) 645, 654, 704, 1114, 1319, 1364
HpyF3I CTNAG 7 cut(s) 125, 462, 681, 882, 1013, 1025, 1176
HpySE526I ACGT 2 cut(s) 530, 1221
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 8 cut(s) 338, 461, 625, 728, 1093, 1137, 1255, 1290
HspAI GCGC 1 cut(s) 915
Ksp22I TGATCA 2 cut(s) 1206, 1273
Kzo9I GATC 5 cut(s) 433, 574, 1140, 1206, 1273
LmnI GCTCC 3 cut(s) 318, 1005, 1114
Lsp1109I GCAGC 6 cut(s) 98, 224, 790, 1226, 1295, 1306
LweI GCATC 2 cut(s) 1087, 1320
MaeI CTAG 4 cut(s) 230, 513, 867, 1109
MaeII ACGT 2 cut(s) 530, 1221
MaeIII GTNAC 3 cut(s) 58, 853, 1217
MalI GATC 5 cut(s) 435, 576, 1142, 1208, 1275
MboI GATC 5 cut(s) 433, 574, 1140, 1206, 1273
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 1341
MluCI AATT 7 cut(s) 43, 142, 147, 625, 738, 910, 1154
MlyI GAGTC 3 cut(s) 914, 1017, 1086
MmeI TCCRAC 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 359, 483, 1361
MspA1I CMGCKG 1 cut(s) 886
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 3 cut(s) 96, 348, 709
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 2 cut(s) 348, 709
MwoI GCNNNNNNNGC 6 cut(s) 645, 654, 704, 1114, 1319, 1364
NciI CCSGG 1 cut(s) 96
NdeII GATC 5 cut(s) 433, 574, 1140, 1206, 1273
NlaIII CATG 8 cut(s) 338, 461, 625, 728, 1093, 1137, 1255, 1290
NlaIV GGNNCC 3 cut(s) 100, 1323, 1338
NmeAIII GCCGAG 1 cut(s) 1289
NmuCI GTSAC 2 cut(s) 58, 853
NspI RCATGY 2 cut(s) 461, 1290
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 4 cut(s) 27, 161, 520, 1129
PflFI GACNNNGTC 1 cut(s) 791
PflMI CCANNNNNTGG 2 cut(s) 283, 1169
PkrI GCNGC 7 cut(s) 52, 88, 214, 805, 1241, 1285, 1296
PleI GAGTC 3 cut(s) 913, 1017, 1085
PpsI GAGTC 3 cut(s) 913, 1017, 1085
Psp1406I AACGTT 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 346, 707
PspGI CCWGG 2 cut(s) 346, 707
PspN4I GGNNCC 3 cut(s) 100, 1323, 1338
PstNI CAGNNNCTG 1 cut(s) 960
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 791
PvuII CAGCTG 1 cut(s) 886
RsaI GTAC 3 cut(s) 239, 380, 1043
RsaNI GTAC 3 cut(s) 238, 379, 1042
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 359, 483, 1361
SatI GCNGC 7 cut(s) 51, 87, 213, 804, 1240, 1284, 1295
Sau3AI GATC 5 cut(s) 433, 574, 1140, 1206, 1273
SchI GAGTC 3 cut(s) 914, 1017, 1086
ScrFI CCNGG 3 cut(s) 96, 348, 709
SduI GDGCHC 1 cut(s) 1341
SfaNI GCATC 2 cut(s) 1087, 1320
SmlI CTYRAG 2 cut(s) 437, 1004
SmoI CTYRAG 2 cut(s) 437, 1004
SpeI ACTAGT 1 cut(s) 866
Sse9I AATT 7 cut(s) 43, 142, 147, 625, 738, 910, 1154
SsiI CCGC 8 cut(s) 48, 51, 847, 1255, 1313, 1333, 1351, 1356
SspMI CTAG 4 cut(s) 230, 513, 867, 1109
StyD4I CCNGG 3 cut(s) 94, 346, 707
TaaI ACNGT 6 cut(s) 134, 559, 592, 859, 1169, 1246
TaiI ACGT 2 cut(s) 533, 1224
TaqI TCGA 4 cut(s) 5, 577, 602, 863
TaqII GACCGA 1 cut(s) 1205
TasI AATT 7 cut(s) 43, 142, 147, 625, 738, 910, 1154
TatI WGTACW 2 cut(s) 378, 1041
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 4 cut(s) 27, 161, 520, 1129
Tru1I TTAA 3 cut(s) 359, 483, 1361
Tru9I TTAA 3 cut(s) 359, 483, 1361
TscAI CASTG 4 cut(s) 649, 1174, 1249, 1332
TseFI GTSAC 2 cut(s) 58, 853
TseI GCWGC 6 cut(s) 86, 212, 803, 1239, 1283, 1294
Tsp45I GTSAC 2 cut(s) 58, 853
TspDTI ATGAA 4 cut(s) 426, 638, 741, 1171
TspGWI ACGGA 2 cut(s) 54, 890
TspRI CASTG 4 cut(s) 649, 1174, 1249, 1332
Tth111I GACNNNGTC 1 cut(s) 791
Van91I CCANNNNNTGG 2 cut(s) 283, 1169
XceI RCATGY 2 cut(s) 461, 1290
XmiI GTMKAC 2 cut(s) 5, 819
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 4 cut(s) 230, 513, 867, 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.