Rw4G035100

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015306
Physical Location & Seq
Forward (+)
61950577 .. 61952964
2388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035100.1

Sequence Viewer

Length: 1371 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTCCGATCGAACCCTCTACCTCTCCACAAGGCTCCCACGCCCTCTCCTCTCTCTTCTCGATTCCTGAACCACTCACCACCGCTCCTCATTCCCCTCGTTTCGTCCCACCGCCTCTCGTTTTCCACAGCCACCGCCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTGCCCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAAGCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTATCGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATCATATTTGGGCGCATCACTGTGATCAGTTGGAAGGTTTAAACAAAGATTTTAGGAAGATGTTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTGAAGCAAATTATTACCGCAGCGGATGGAACTAGGAAGATTTTATTCACTTTGGAAGATGGGATGGTGATTGAAACCGTTGTCATACCTTCTGATAGTAGCCGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGGCATGAATTGCCAGTTCTGCTACACCGGCAGGATGGGTCTAAGGAGACATCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGCGAGATTACCAATGTTGTATTCATGGGAATGGGAGAACCACTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTGCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTATTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGTAAGATCAACCTTATCTCATTTAATCCTCATAGCGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGCGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCCTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAAGCTCCCATGCTCCGCGTGCCAGAGAAGTTCAAAACGGCAACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

456

Amino Acids

50.84

Weight (kDa)

8.97

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 199 - 368 2.7e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 210
AccII CGCG 3 cut(s) 703, 1236, 1329
AclWI GGATC 4 cut(s) 699, 965, 1161, 1174
AcoI YGGCCR 2 cut(s) 343, 1250
AfiI CCNNNNNNNGG 3 cut(s) 230, 1163, 1259
AjnI CCWGG 1 cut(s) 1300
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
Alw26I GTCTC 4 cut(s) 25, 262, 374, 652
AlwI GGATC 4 cut(s) 699, 965, 1161, 1174
AlwNI CAGNNNCTG 1 cut(s) 954
AoxI GGCC 6 cut(s) 338, 343, 823, 1231, 1250, 1278
ApeKI GCWGC 4 cut(s) 18, 494, 671, 797
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 2 cut(s) 394, 703
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 3 cut(s) 108, 245, 553
BamHI GGATCC 1 cut(s) 1166
BbvCI CCTCAGC 2 cut(s) 876, 881
BbvI GCAGC 4 cut(s) 5, 506, 683, 784
BccI CCATC 5 cut(s) 494, 527, 532, 640, 1267
BceAI ACGGC 2 cut(s) 974, 1365
BciT130I CCWGG 1 cut(s) 1302
BclI TGATCA 3 cut(s) 403, 817, 1267
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 4 cut(s) 25, 262, 374, 652
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 4 cut(s) 669, 798, 912, 936
BisI GCNGC 8 cut(s) 19, 177, 495, 672, 798, 1234, 1250, 1278
BlsI GCNGC 8 cut(s) 20, 178, 496, 673, 799, 1235, 1251, 1279
Bme1390I CCNGG 2 cut(s) 342, 1302
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 75, 1168
BmrFI CCNGG 2 cut(s) 342, 1302
BmsI GCATC 4 cut(s) 282, 345, 403, 1081
BpmI CTGGAG 2 cut(s) 273, 1245
Bpu10I CCTNAGC 2 cut(s) 876, 881
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 108, 138
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 1163, 1259
Bse118I RCCGGY 1 cut(s) 638
Bse1I ACTGG 3 cut(s) 625, 959, 1185
BseBI CCWGG 1 cut(s) 1302
BseGI GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BseLI CCNNNNNNNGG 3 cut(s) 230, 1163, 1259
BseMII CTCAG 4 cut(s) 666, 872, 890, 907
BseNI ACTGG 3 cut(s) 625, 959, 1185
BseRI GAGGAG 2 cut(s) 78, 116
BseX3I CGGCCG 1 cut(s) 1250
BseXI GCAGC 4 cut(s) 5, 506, 683, 784
Bsh1236I CGCG 3 cut(s) 703, 1236, 1329
Bsh1285I CGRYCG 2 cut(s) 50, 1253
BshFI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BsiEI CGRYCG 2 cut(s) 50, 1253
BsiSI CCGG 4 cut(s) 342, 577, 639, 973
BslFI GGGAC 2 cut(s) 130, 857
BslI CCNNNNNNNGG 3 cut(s) 230, 1163, 1259
BsmAI GTCTC 4 cut(s) 25, 262, 374, 652
BsmBI CGTCTC 2 cut(s) 25, 374
BsmFI GGGAC 2 cut(s) 130, 857
BsmI GAATGC 3 cut(s) 457, 934, 1122
BsnI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
Bsp143I GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
BspANI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BspCNI CTCAG 4 cut(s) 667, 873, 889, 906
BspFNI CGCG 3 cut(s) 703, 1236, 1329
BspLI GGNNCC 2 cut(s) 75, 1168
BspMAI CTGCAG 2 cut(s) 673, 802
BspPI GGATC 4 cut(s) 699, 965, 1161, 1174
BsrBI CCGCTC 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 638
BsrI ACTGG 3 cut(s) 625, 959, 1185
BssAI RCCGGY 1 cut(s) 638
BssMI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
Bst2UI CCWGG 1 cut(s) 1302
Bst4CI ACNGT 4 cut(s) 401, 553, 586, 853
Bst6I CTCTTC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 621
BstC8I GCNNGC 6 cut(s) 690, 827, 1065, 1104, 1231, 1331
BstDEI CTNAG 5 cut(s) 653, 675, 876, 881, 893
BstF5I GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BstFNI CGCG 3 cut(s) 703, 1236, 1329
BstHHI GCGC 2 cut(s) 394, 703
BstKTI GATC 9 cut(s) 50, 406, 707, 820, 960, 1111, 1137, 1169, 1270
BstMAI GTCTC 4 cut(s) 25, 262, 374, 652
BstMBI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
BstMCI CGRYCG 2 cut(s) 50, 1253
BstMWI GCNNNNNNNGC 8 cut(s) 176, 621, 630, 639, 698, 709, 1302, 1330
BstNI CCWGG 1 cut(s) 1302
BstSCI CCNGG 2 cut(s) 340, 1300
BstSFI CTRYAG 4 cut(s) 669, 798, 912, 936
BstUI CGCG 3 cut(s) 703, 1236, 1329
BstV1I GCAGC 4 cut(s) 5, 506, 683, 784
BstX2I RGATCY 1 cut(s) 1166
BstYI RGATCY 1 cut(s) 1166
BstZI CGGCCG 1 cut(s) 1250
BsuRI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BtgZI GCGATG 1 cut(s) 1278
BtsCI GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BtsIMutI CAGTG 2 cut(s) 397, 1192
Cac8I GCNNGC 6 cut(s) 690, 827, 1065, 1104, 1231, 1331
CaiI CAGNNNCTG 1 cut(s) 954
CfoI GCGC 2 cut(s) 394, 703
Cfr10I RCCGGY 1 cut(s) 638
Cfr13I GGNCC 1 cut(s) 339
CspCI CAANNNNNGTGG 3 cut(s) 585, 620, 1341
CviAII CATG 6 cut(s) 616, 751, 961, 1128, 1246, 1321
DdeI CTNAG 5 cut(s) 653, 675, 876, 881, 893
DpnI GATC 9 cut(s) 49, 405, 706, 819, 959, 1110, 1136, 1168, 1269
DpnII GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
DraI TTTAAA 1 cut(s) 420
EaeI YGGCCR 2 cut(s) 343, 1250
EagI CGGCCG 1 cut(s) 1250
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 1250
Eco147I AGGCCT 1 cut(s) 825
Eco52I CGGCCG 1 cut(s) 1250
EcoRII CCWGG 1 cut(s) 1300
Esp3I CGTCTC 2 cut(s) 25, 374
FaeI CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 2 cut(s) 130, 857
FatI CATG 6 cut(s) 615, 750, 960, 1127, 1245, 1320
FauI CCCGC 1 cut(s) 232
FbaI TGATCA 3 cut(s) 403, 817, 1267
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 8 cut(s) 19, 177, 495, 672, 798, 1234, 1250, 1278
FokI GGATG 7 cut(s) 40, 304, 512, 550, 658, 827, 1103
Fsp4HI GCNGC 8 cut(s) 19, 177, 495, 672, 798, 1234, 1250, 1278
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 393, 702
GluI GCNGC 8 cut(s) 19, 177, 495, 672, 798, 1234, 1250, 1278
GsuI CTGGAG 2 cut(s) 273, 1245
HaeIII GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
HapII CCGG 4 cut(s) 342, 577, 639, 973
HhaI GCGC 2 cut(s) 394, 703
Hin1II CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
Hin6I GCGC 2 cut(s) 392, 701
HinP1I GCGC 2 cut(s) 392, 701
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 6 cut(s) 36, 102, 255, 379, 899, 1003
HpaII CCGG 4 cut(s) 342, 577, 639, 973
HphI GGTGA 3 cut(s) 108, 245, 553
Hpy166II GTNNAC 2 cut(s) 211, 246
Hpy188I TCNGA 9 cut(s) 47, 568, 666, 896, 950, 1002, 1178, 1211, 1272
Hpy188III TCNNGA 4 cut(s) 99, 106, 259, 1027
Hpy8I GTNNAC 2 cut(s) 211, 246
Hpy99I CGWCG 2 cut(s) 212, 215
HpyAV CCTTC 4 cut(s) 249, 268, 407, 573
HpyCH4III ACNGT 4 cut(s) 401, 553, 586, 853
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 6 cut(s) 671, 800, 829, 932, 1067, 1094
HpyF10VI GCNNNNNNNGC 8 cut(s) 176, 621, 630, 639, 698, 709, 1302, 1330
HpyF3I CTNAG 5 cut(s) 653, 675, 876, 881, 893
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
HspAI GCGC 2 cut(s) 392, 701
Ksp22I TGATCA 3 cut(s) 403, 817, 1267
Kzo9I GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
LmnI GCTCC 5 cut(s) 79, 129, 1226, 1321, 1329
Lsp1109I GCAGC 4 cut(s) 5, 506, 683, 784
LweI GCATC 4 cut(s) 282, 345, 403, 1081
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 2 cut(s) 887, 1187
MalI GATC 9 cut(s) 49, 405, 706, 819, 959, 1110, 1136, 1168, 1269
MbiI CCGCTC 1 cut(s) 124
MboI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
MboII GAAGA 5 cut(s) 87, 448, 523, 542, 989
MflI RGATCY 1 cut(s) 1166
MluCI AATT 5 cut(s) 483, 619, 904, 966, 1213
MlyI GAGTC 3 cut(s) 264, 908, 997
MmeI TCCRAC 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
MslI CAYNNNNRTG 3 cut(s) 399, 755, 809
MspA1I CMGCKG 4 cut(s) 251, 497, 674, 880
MspI CCGG 4 cut(s) 342, 577, 639, 973
MspR9I CCNGG 2 cut(s) 342, 1302
MssI GTTTAAAC 1 cut(s) 420
Mva1269I GAATGC 3 cut(s) 457, 934, 1122
MvaI CCWGG 1 cut(s) 1302
MvnI CGCG 3 cut(s) 703, 1236, 1329
MwoI GCNNNNNNNGC 8 cut(s) 176, 621, 630, 639, 698, 709, 1302, 1330
NciI CCSGG 1 cut(s) 342
NdeII GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
NlaIII CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
NlaIV GGNNCC 2 cut(s) 75, 1168
NmeAIII GCCGAG 2 cut(s) 481, 1278
NmuCI GTSAC 1 cut(s) 1187
PceI AGGCCT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 3 cut(s) 457, 934, 1122
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 3 cut(s) 36, 102, 379
PkrI GCNGC 8 cut(s) 20, 178, 496, 673, 799, 1235, 1251, 1279
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 263, 907, 997
PmeI GTTTAAAC 1 cut(s) 420
PpsI GAGTC 3 cut(s) 263, 907, 997
Psp6I CCWGG 1 cut(s) 1300
PspGI CCWGG 1 cut(s) 1300
PspN4I GGNNCC 2 cut(s) 75, 1168
PspPI GGNCC 1 cut(s) 339
PstI CTGCAG 2 cut(s) 673, 802
PstNI CAGNNNCTG 1 cut(s) 954
PsuI RGATCY 1 cut(s) 1166
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 3 cut(s) 251, 674, 880
RseI CAYNNNNRTG 3 cut(s) 399, 755, 809
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
SatI GCNGC 8 cut(s) 19, 177, 495, 672, 798, 1234, 1250, 1278
Sau3AI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
Sau96I GGNCC 1 cut(s) 339
SchI GAGTC 3 cut(s) 264, 908, 997
ScrFI CCNGG 2 cut(s) 342, 1302
SfaNI GCATC 4 cut(s) 282, 345, 403, 1081
SfcI CTRYAG 4 cut(s) 669, 798, 912, 936
SgrDI CGTCGACG 1 cut(s) 209
SmiMI CAYNNNNRTG 3 cut(s) 399, 755, 809
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 5 cut(s) 483, 619, 904, 966, 1213
SseBI AGGCCT 1 cut(s) 825
SspMI CTAG 1 cut(s) 507
StuI AGGCCT 1 cut(s) 825
StyD4I CCNGG 2 cut(s) 340, 1300
TaaI ACNGT 4 cut(s) 401, 553, 586, 853
TaiI ACGT 1 cut(s) 370
TaqI TCGA 5 cut(s) 50, 100, 210, 260, 272
TasI AATT 5 cut(s) 483, 619, 904, 966, 1213
TauI GCSGC 4 cut(s) 179, 1236, 1252, 1280
TfiI GAWTC 3 cut(s) 36, 102, 379
Tru1I TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
Tru9I TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
TscAI CASTG 2 cut(s) 404, 1192
TseFI GTSAC 1 cut(s) 1187
TseI GCWGC 4 cut(s) 18, 494, 671, 797
Tsp45I GTSAC 1 cut(s) 1187
TspDTI ATGAA 3 cut(s) 632, 739, 1234
TspGWI ACGGA 2 cut(s) 23, 196
TspRI CASTG 2 cut(s) 404, 1192
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.