RchiOBHm_Chr4g0443901

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015306
Physical Location & Seq
Forward (+)
64854881 .. 64857574
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41160

Sequence Viewer

Length: 1326 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTCCGATCGAACCCTCTACCTCTCTCCTCTCGATTCCTGAAACACTCACCACCGCTCCTCATTCCTCTCGTTTCGTCCCACCGCCTCTCGTTTTCCACAGCCACCACCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTGCCCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAAGCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACAGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATTATATTTGGGCGCATCACAGTGATCAGTTGGAAGGTTTAAACAAAGATTTTAGGAAGATGCTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTGAAGCAAATTATTACCGCAGCCGATGGAACTAGGAAGATTTTATTCACTTTGGAAGATGGGATGGTGATTGAAACCGTTGTCATACCTTCTGATAGTAGCAGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGGCATGAATTGCCAGATGGGTCTAAGGAGACATCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGCGAGATTACCAATGTTGTATTTATGGGAATGGGAGAACCGCTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTATTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTTATCTCGTTTAATCCTCATAGTGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGCGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCCTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAAGCTCCCATGCTCCGCGTGCCAGAGAAGTTCAAAACGGCAACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

441

Amino Acids

49.24

Weight (kDa)

8.97

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 201 - 353 1.4e-09 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 183
AccII CGCG 3 cut(s) 658, 1191, 1284
AclWI GGATC 4 cut(s) 654, 920, 1116, 1129
AcoI YGGCCR 2 cut(s) 316, 1205
AfiI CCNNNNNNNGG 3 cut(s) 203, 1118, 1214
AjnI CCWGG 1 cut(s) 1255
Alw26I GTCTC 4 cut(s) 25, 235, 347, 607
AlwI GGATC 4 cut(s) 654, 920, 1116, 1129
AlwNI CAGNNNCTG 1 cut(s) 909
AoxI GGCC 6 cut(s) 311, 316, 778, 1186, 1205, 1233
ApeKI GCWGC 4 cut(s) 18, 467, 626, 752
Asp700I GAANNNNTTC 1 cut(s) 723
AspLEI GCGC 2 cut(s) 367, 658
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 3 cut(s) 81, 218, 526
BamHI GGATCC 1 cut(s) 1121
BbvCI CCTCAGC 2 cut(s) 831, 836
BbvI GCAGC 4 cut(s) 5, 479, 638, 739
BccI CCATC 5 cut(s) 467, 500, 505, 595, 1222
BceAI ACGGC 2 cut(s) 929, 1320
BciT130I CCWGG 1 cut(s) 1257
BclI TGATCA 3 cut(s) 376, 772, 1222
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 4 cut(s) 25, 235, 347, 607
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 5 cut(s) 307, 624, 753, 867, 891
BisI GCNGC 7 cut(s) 19, 468, 627, 753, 1189, 1205, 1233
BlsI GCNGC 7 cut(s) 20, 469, 628, 754, 1190, 1206, 1234
Bme1390I CCNGG 2 cut(s) 315, 1257
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 1 cut(s) 1123
BmrFI CCNGG 2 cut(s) 315, 1257
BmsI GCATC 5 cut(s) 255, 318, 376, 402, 1036
BpmI CTGGAG 2 cut(s) 246, 1200
Bpu10I CCTNAGC 2 cut(s) 831, 836
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 927
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 1118, 1214
Bse1I ACTGG 2 cut(s) 914, 1140
BseBI CCWGG 1 cut(s) 1257
BseGI GGATG 5 cut(s) 33, 270, 516, 775, 1051
BseLI CCNNNNNNNGG 3 cut(s) 203, 1118, 1214
BseMII CTCAG 5 cut(s) 407, 621, 827, 845, 862
BseNI ACTGG 2 cut(s) 914, 1140
BseRI GAGGAG 2 cut(s) 58, 89
BseX3I CGGCCG 1 cut(s) 1205
BseXI GCAGC 4 cut(s) 5, 479, 638, 739
Bsh1236I CGCG 3 cut(s) 658, 1191, 1284
Bsh1285I CGRYCG 2 cut(s) 50, 1208
BshFI GGCC 6 cut(s) 313, 318, 780, 1188, 1207, 1235
BsiEI CGRYCG 2 cut(s) 50, 1208
BsiSI CCGG 2 cut(s) 315, 928
BslFI GGGAC 2 cut(s) 103, 812
BslI CCNNNNNNNGG 3 cut(s) 203, 1118, 1214
BsmAI GTCTC 4 cut(s) 25, 235, 347, 607
BsmBI CGTCTC 2 cut(s) 25, 347
BsmFI GGGAC 2 cut(s) 103, 812
BsmI GAATGC 3 cut(s) 430, 889, 1077
BsnI GGCC 6 cut(s) 313, 318, 780, 1188, 1207, 1235
Bsp143I GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
BspANI GGCC 6 cut(s) 313, 318, 780, 1188, 1207, 1235
BspCNI CTCAG 5 cut(s) 408, 622, 828, 844, 861
BspFNI CGCG 3 cut(s) 658, 1191, 1284
BspLI GGNNCC 1 cut(s) 1123
BspMAI CTGCAG 2 cut(s) 628, 757
BspPI GGATC 4 cut(s) 654, 920, 1116, 1129
BsrBI CCGCTC 1 cut(s) 97
BsrI ACTGG 2 cut(s) 914, 1140
BssMI GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
Bst2UI CCWGG 1 cut(s) 1257
Bst4CI ACNGT 4 cut(s) 374, 526, 559, 808
BstAPI GCANNNNNTGC 2 cut(s) 594, 1083
BstC8I GCNNGC 5 cut(s) 645, 1020, 1059, 1186, 1286
BstDEI CTNAG 6 cut(s) 416, 608, 630, 831, 836, 848
BstF5I GGATG 5 cut(s) 33, 270, 516, 775, 1051
BstFNI CGCG 3 cut(s) 658, 1191, 1284
BstHHI GCGC 2 cut(s) 367, 658
BstKTI GATC 9 cut(s) 50, 379, 662, 775, 915, 1066, 1092, 1124, 1225
BstMAI GTCTC 4 cut(s) 25, 235, 347, 607
BstMBI GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
BstMCI CGRYCG 2 cut(s) 50, 1208
BstMWI GCNNNNNNNGC 7 cut(s) 149, 594, 653, 664, 1083, 1257, 1285
BstNI CCWGG 1 cut(s) 1257
BstSCI CCNGG 2 cut(s) 313, 1255
BstSFI CTRYAG 5 cut(s) 307, 624, 753, 867, 891
BstUI CGCG 3 cut(s) 658, 1191, 1284
BstV1I GCAGC 4 cut(s) 5, 479, 638, 739
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BstZI CGGCCG 1 cut(s) 1205
BsuRI GGCC 6 cut(s) 313, 318, 780, 1188, 1207, 1235
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 5 cut(s) 33, 270, 516, 775, 1051
BtsIMutI CAGTG 2 cut(s) 379, 1147
Cac8I GCNNGC 5 cut(s) 645, 1020, 1059, 1186, 1286
CaiI CAGNNNCTG 1 cut(s) 909
CfoI GCGC 2 cut(s) 367, 658
Cfr13I GGNCC 1 cut(s) 312
CspCI CAANNNNNGTGG 3 cut(s) 558, 593, 1296
CviAII CATG 5 cut(s) 589, 916, 1083, 1201, 1276
DdeI CTNAG 6 cut(s) 416, 608, 630, 831, 836, 848
DpnI GATC 9 cut(s) 49, 378, 661, 774, 914, 1065, 1091, 1123, 1224
DpnII GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
DraI TTTAAA 1 cut(s) 393
EaeI YGGCCR 2 cut(s) 316, 1205
EagI CGGCCG 1 cut(s) 1205
EclXI CGGCCG 1 cut(s) 1205
Eco147I AGGCCT 1 cut(s) 780
Eco52I CGGCCG 1 cut(s) 1205
EcoRII CCWGG 1 cut(s) 1255
Esp3I CGTCTC 2 cut(s) 25, 347
FaeI CATG 5 cut(s) 592, 919, 1086, 1204, 1279
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 2 cut(s) 103, 812
FatI CATG 5 cut(s) 588, 915, 1082, 1200, 1275
FauI CCCGC 1 cut(s) 205
FbaI TGATCA 3 cut(s) 376, 772, 1222
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 7 cut(s) 19, 468, 627, 753, 1189, 1205, 1233
FokI GGATG 5 cut(s) 40, 277, 523, 782, 1058
Fsp4HI GCNGC 7 cut(s) 19, 468, 627, 753, 1189, 1205, 1233
FspBI CTAG 1 cut(s) 480
GlaI GCGC 2 cut(s) 366, 657
GluI GCNGC 7 cut(s) 19, 468, 627, 753, 1189, 1205, 1233
GsuI CTGGAG 2 cut(s) 246, 1200
HaeIII GGCC 6 cut(s) 313, 318, 780, 1188, 1207, 1235
HapII CCGG 2 cut(s) 315, 928
HhaI GCGC 2 cut(s) 367, 658
Hin1II CATG 5 cut(s) 592, 919, 1086, 1204, 1279
Hin6I GCGC 2 cut(s) 365, 656
HinP1I GCGC 2 cut(s) 365, 656
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 5 cut(s) 36, 75, 228, 854, 958
HpaII CCGG 2 cut(s) 315, 928
HphI GGTGA 3 cut(s) 81, 218, 526
Hpy166II GTNNAC 2 cut(s) 184, 219
Hpy188I TCNGA 9 cut(s) 47, 541, 621, 851, 905, 957, 1133, 1166, 1227
Hpy188III TCNNGA 4 cut(s) 72, 79, 232, 982
Hpy8I GTNNAC 2 cut(s) 184, 219
Hpy99I CGWCG 2 cut(s) 185, 188
HpyAV CCTTC 5 cut(s) 222, 241, 380, 546, 791
HpyCH4III ACNGT 4 cut(s) 374, 526, 559, 808
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 6 cut(s) 626, 755, 887, 1022, 1049, 1086
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 594, 653, 664, 1083, 1257, 1285
HpyF3I CTNAG 6 cut(s) 416, 608, 630, 831, 836, 848
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 5 cut(s) 592, 919, 1086, 1204, 1279
HspAI GCGC 2 cut(s) 365, 656
Ksp22I TGATCA 3 cut(s) 376, 772, 1222
Kzo9I GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
LmnI GCTCC 4 cut(s) 102, 1181, 1276, 1284
Lsp1109I GCAGC 4 cut(s) 5, 479, 638, 739
LweI GCATC 5 cut(s) 255, 318, 376, 402, 1036
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 2 cut(s) 842, 1142
MalI GATC 9 cut(s) 49, 378, 661, 774, 914, 1065, 1091, 1123, 1224
MbiI CCGCTC 1 cut(s) 97
MboI GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
MboII GAAGA 4 cut(s) 421, 496, 515, 944
MflI RGATCY 1 cut(s) 1121
MluCI AATT 6 cut(s) 352, 456, 592, 859, 921, 1168
MlyI GAGTC 3 cut(s) 237, 863, 952
MmeI TCCRAC 1 cut(s) 363
MroXI GAANNNNTTC 1 cut(s) 723
MseI TTAA 6 cut(s) 258, 392, 747, 924, 963, 1107
MslI CAYNNNNRTG 2 cut(s) 372, 764
MspA1I CMGCKG 3 cut(s) 224, 629, 835
MspI CCGG 2 cut(s) 315, 928
MspR9I CCNGG 2 cut(s) 315, 1257
MssI GTTTAAAC 1 cut(s) 393
Mva1269I GAATGC 3 cut(s) 430, 889, 1077
MvaI CCWGG 1 cut(s) 1257
MvnI CGCG 3 cut(s) 658, 1191, 1284
MwoI GCNNNNNNNGC 7 cut(s) 149, 594, 653, 664, 1083, 1257, 1285
NciI CCSGG 1 cut(s) 315
NdeII GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
NlaIII CATG 5 cut(s) 592, 919, 1086, 1204, 1279
NlaIV GGNNCC 1 cut(s) 1123
NmeAIII GCCGAG 2 cut(s) 454, 1233
NmuCI GTSAC 1 cut(s) 1142
PceI AGGCCT 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 3 cut(s) 430, 889, 1077
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 2 cut(s) 36, 75
PkrI GCNGC 7 cut(s) 20, 469, 628, 754, 1190, 1206, 1234
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 236, 862, 952
PmeI GTTTAAAC 1 cut(s) 393
PpsI GAGTC 3 cut(s) 236, 862, 952
Psp6I CCWGG 1 cut(s) 1255
PspGI CCWGG 1 cut(s) 1255
PspN4I GGNNCC 1 cut(s) 1123
PspPI GGNCC 1 cut(s) 312
PstI CTGCAG 2 cut(s) 628, 757
PstNI CAGNNNCTG 1 cut(s) 909
PsuI RGATCY 1 cut(s) 1121
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 3 cut(s) 224, 629, 835
RseI CAYNNNNRTG 2 cut(s) 372, 764
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 6 cut(s) 258, 392, 747, 924, 963, 1107
SatI GCNGC 7 cut(s) 19, 468, 627, 753, 1189, 1205, 1233
Sau3AI GATC 9 cut(s) 47, 376, 659, 772, 912, 1063, 1089, 1121, 1222
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 3 cut(s) 237, 863, 952
ScrFI CCNGG 2 cut(s) 315, 1257
SfaNI GCATC 5 cut(s) 255, 318, 376, 402, 1036
SfcI CTRYAG 5 cut(s) 307, 624, 753, 867, 891
SgrDI CGTCGACG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 372, 764
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 6 cut(s) 352, 456, 592, 859, 921, 1168
SseBI AGGCCT 1 cut(s) 780
SspMI CTAG 1 cut(s) 480
StuI AGGCCT 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 313, 1255
TaaI ACNGT 4 cut(s) 374, 526, 559, 808
TaiI ACGT 1 cut(s) 343
TaqI TCGA 5 cut(s) 50, 73, 183, 233, 245
TasI AATT 6 cut(s) 352, 456, 592, 859, 921, 1168
TauI GCSGC 3 cut(s) 1191, 1207, 1235
TfiI GAWTC 2 cut(s) 36, 75
Tru1I TTAA 6 cut(s) 258, 392, 747, 924, 963, 1107
Tru9I TTAA 6 cut(s) 258, 392, 747, 924, 963, 1107
TscAI CASTG 2 cut(s) 379, 1147
TseFI GTSAC 1 cut(s) 1142
TseI GCWGC 4 cut(s) 18, 467, 626, 752
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 3 cut(s) 605, 773, 1189
TspGWI ACGGA 2 cut(s) 23, 169
TspRI CASTG 2 cut(s) 379, 1147
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 723
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.