Rh4CG433800

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015306
Physical Location & Seq
Forward (+)
67305856 .. 67308233
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG433800.1

Sequence Viewer

Length: 1344 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTCCGATCGAACCCTCTACCTCTCTCCTCTCGATTCCTGAAACACTCACCACCGCTCCTCATTCCTCTCGTTTCGTCCCACCGCCTCTCGTTTTCCACAGCCACCACCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTGCCCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAAGCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACAGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATTATATTTGGGCGCATCACAGTGATCAGTTGGAAGGTTTAAACAAAGATTTTAGGAAGATGCTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTGAAGCAAATTATTACCGCAGCCGATGGAACTAGGAAGATTTTATTCACTTTGGAAGATGGGATGGTGATTGAAACCGTTGTCATACCTTCTGATAGTAGCAGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGGCATGAATTGCCAGTTCTGCTACACCGGCAGGATGGGTCTAAGGAGACATCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGCGAGATTACCAATGTTGTATTCATGGGAATGGGAGAACCGCTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTATTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTTATCTCATTTAATCCTCATAGTGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGCGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCCTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAAGCTCCCATGCTCCGCGTGCCAGAGAAGTTCAAAACGGCAACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

447

Amino Acids

49.96

Weight (kDa)

9.01

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 190 - 359 2.6e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 97
AccI GTMKAC 1 cut(s) 183
AccII CGCG 3 cut(s) 676, 1209, 1302
AclWI GGATC 4 cut(s) 672, 938, 1134, 1147
AcoI YGGCCR 2 cut(s) 316, 1223
AfiI CCNNNNNNNGG 3 cut(s) 203, 1136, 1232
AjnI CCWGG 1 cut(s) 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
Alw26I GTCTC 4 cut(s) 25, 235, 347, 625
AlwI GGATC 4 cut(s) 672, 938, 1134, 1147
AlwNI CAGNNNCTG 1 cut(s) 927
AoxI GGCC 6 cut(s) 311, 316, 796, 1204, 1223, 1251
ApeKI GCWGC 4 cut(s) 18, 467, 644, 770
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 2 cut(s) 367, 676
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 3 cut(s) 81, 218, 526
BamHI GGATCC 1 cut(s) 1139
BbvCI CCTCAGC 2 cut(s) 849, 854
BbvI GCAGC 4 cut(s) 5, 479, 656, 757
BccI CCATC 5 cut(s) 467, 500, 505, 613, 1240
BceAI ACGGC 2 cut(s) 947, 1338
BciT130I CCWGG 1 cut(s) 1275
BclI TGATCA 3 cut(s) 376, 790, 1240
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 4 cut(s) 25, 235, 347, 625
BfaI CTAG 1 cut(s) 480
BfmI CTRYAG 5 cut(s) 307, 642, 771, 885, 909
BisI GCNGC 7 cut(s) 19, 468, 645, 771, 1207, 1223, 1251
BlsI GCNGC 7 cut(s) 20, 469, 646, 772, 1208, 1224, 1252
Bme1390I CCNGG 2 cut(s) 315, 1275
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 1 cut(s) 1141
BmrFI CCNGG 2 cut(s) 315, 1275
BmsI GCATC 5 cut(s) 255, 318, 376, 402, 1054
BpmI CTGGAG 2 cut(s) 246, 1218
Bpu10I CCTNAGC 2 cut(s) 849, 854
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 945
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 1136, 1232
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 3 cut(s) 598, 932, 1158
BseBI CCWGG 1 cut(s) 1275
BseGI GGATG 6 cut(s) 33, 270, 516, 624, 793, 1069
BseLI CCNNNNNNNGG 3 cut(s) 203, 1136, 1232
BseMII CTCAG 5 cut(s) 407, 639, 845, 863, 880
BseNI ACTGG 3 cut(s) 598, 932, 1158
BseRI GAGGAG 2 cut(s) 58, 89
BseX3I CGGCCG 1 cut(s) 1223
BseXI GCAGC 4 cut(s) 5, 479, 656, 757
Bsh1236I CGCG 3 cut(s) 676, 1209, 1302
Bsh1285I CGRYCG 2 cut(s) 50, 1226
BshFI GGCC 6 cut(s) 313, 318, 798, 1206, 1225, 1253
BsiEI CGRYCG 2 cut(s) 50, 1226
BsiSI CCGG 3 cut(s) 315, 612, 946
BslFI GGGAC 2 cut(s) 103, 830
BslI CCNNNNNNNGG 3 cut(s) 203, 1136, 1232
BsmAI GTCTC 4 cut(s) 25, 235, 347, 625
BsmBI CGTCTC 2 cut(s) 25, 347
BsmFI GGGAC 2 cut(s) 103, 830
BsmI GAATGC 3 cut(s) 430, 907, 1095
BsnI GGCC 6 cut(s) 313, 318, 798, 1206, 1225, 1253
Bsp143I GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
BspANI GGCC 6 cut(s) 313, 318, 798, 1206, 1225, 1253
BspCNI CTCAG 5 cut(s) 408, 640, 846, 862, 879
BspFNI CGCG 3 cut(s) 676, 1209, 1302
BspLI GGNNCC 1 cut(s) 1141
BspMAI CTGCAG 2 cut(s) 646, 775
BspPI GGATC 4 cut(s) 672, 938, 1134, 1147
BsrBI CCGCTC 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 3 cut(s) 598, 932, 1158
BssAI RCCGGY 1 cut(s) 611
BssMI GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
Bst2UI CCWGG 1 cut(s) 1275
Bst4CI ACNGT 4 cut(s) 374, 526, 559, 826
BstAPI GCANNNNNTGC 2 cut(s) 594, 1101
BstC8I GCNNGC 5 cut(s) 663, 1038, 1077, 1204, 1304
BstDEI CTNAG 6 cut(s) 416, 626, 648, 849, 854, 866
BstF5I GGATG 6 cut(s) 33, 270, 516, 624, 793, 1069
BstFNI CGCG 3 cut(s) 676, 1209, 1302
BstHHI GCGC 2 cut(s) 367, 676
BstKTI GATC 9 cut(s) 50, 379, 680, 793, 933, 1084, 1110, 1142, 1243
BstMAI GTCTC 4 cut(s) 25, 235, 347, 625
BstMBI GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
BstMCI CGRYCG 2 cut(s) 50, 1226
BstMWI GCNNNNNNNGC 9 cut(s) 149, 594, 603, 612, 671, 682, 1101, 1275, 1303
BstNI CCWGG 1 cut(s) 1275
BstSCI CCNGG 2 cut(s) 313, 1273
BstSFI CTRYAG 5 cut(s) 307, 642, 771, 885, 909
BstUI CGCG 3 cut(s) 676, 1209, 1302
BstV1I GCAGC 4 cut(s) 5, 479, 656, 757
BstX2I RGATCY 1 cut(s) 1139
BstYI RGATCY 1 cut(s) 1139
BstZI CGGCCG 1 cut(s) 1223
BsuRI GGCC 6 cut(s) 313, 318, 798, 1206, 1225, 1253
BtgZI GCGATG 1 cut(s) 1251
BtsCI GGATG 6 cut(s) 33, 270, 516, 624, 793, 1069
BtsIMutI CAGTG 2 cut(s) 379, 1165
Cac8I GCNNGC 5 cut(s) 663, 1038, 1077, 1204, 1304
CaiI CAGNNNCTG 1 cut(s) 927
CfoI GCGC 2 cut(s) 367, 676
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 1 cut(s) 312
CspCI CAANNNNNGTGG 3 cut(s) 558, 593, 1314
CviAII CATG 6 cut(s) 589, 724, 934, 1101, 1219, 1294
DdeI CTNAG 6 cut(s) 416, 626, 648, 849, 854, 866
DpnI GATC 9 cut(s) 49, 378, 679, 792, 932, 1083, 1109, 1141, 1242
DpnII GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
DraI TTTAAA 1 cut(s) 393
EaeI YGGCCR 2 cut(s) 316, 1223
EagI CGGCCG 1 cut(s) 1223
EclXI CGGCCG 1 cut(s) 1223
Eco147I AGGCCT 1 cut(s) 798
Eco52I CGGCCG 1 cut(s) 1223
EcoRII CCWGG 1 cut(s) 1273
Esp3I CGTCTC 2 cut(s) 25, 347
FaeI CATG 6 cut(s) 592, 727, 937, 1104, 1222, 1297
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FaqI GGGAC 2 cut(s) 103, 830
FatI CATG 6 cut(s) 588, 723, 933, 1100, 1218, 1293
FauI CCCGC 1 cut(s) 205
FbaI TGATCA 3 cut(s) 376, 790, 1240
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 7 cut(s) 19, 468, 645, 771, 1207, 1223, 1251
FokI GGATG 6 cut(s) 40, 277, 523, 631, 800, 1076
Fsp4HI GCNGC 7 cut(s) 19, 468, 645, 771, 1207, 1223, 1251
FspBI CTAG 1 cut(s) 480
GlaI GCGC 2 cut(s) 366, 675
GluI GCNGC 7 cut(s) 19, 468, 645, 771, 1207, 1223, 1251
GsuI CTGGAG 2 cut(s) 246, 1218
HaeIII GGCC 6 cut(s) 313, 318, 798, 1206, 1225, 1253
HapII CCGG 3 cut(s) 315, 612, 946
HhaI GCGC 2 cut(s) 367, 676
Hin1II CATG 6 cut(s) 592, 727, 937, 1104, 1222, 1297
Hin6I GCGC 2 cut(s) 365, 674
HinP1I GCGC 2 cut(s) 365, 674
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 5 cut(s) 36, 75, 228, 872, 976
HpaII CCGG 3 cut(s) 315, 612, 946
HphI GGTGA 3 cut(s) 81, 218, 526
Hpy166II GTNNAC 2 cut(s) 184, 219
Hpy188I TCNGA 9 cut(s) 47, 541, 639, 869, 923, 975, 1151, 1184, 1245
Hpy188III TCNNGA 4 cut(s) 72, 79, 232, 1000
Hpy8I GTNNAC 2 cut(s) 184, 219
Hpy99I CGWCG 2 cut(s) 185, 188
HpyAV CCTTC 5 cut(s) 222, 241, 380, 546, 809
HpyCH4III ACNGT 4 cut(s) 374, 526, 559, 826
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 6 cut(s) 644, 773, 905, 1040, 1067, 1104
HpyF10VI GCNNNNNNNGC 9 cut(s) 149, 594, 603, 612, 671, 682, 1101, 1275, 1303
HpyF3I CTNAG 6 cut(s) 416, 626, 648, 849, 854, 866
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 6 cut(s) 592, 727, 937, 1104, 1222, 1297
HspAI GCGC 2 cut(s) 365, 674
Ksp22I TGATCA 3 cut(s) 376, 790, 1240
Kzo9I GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
LmnI GCTCC 4 cut(s) 102, 1199, 1294, 1302
Lsp1109I GCAGC 4 cut(s) 5, 479, 656, 757
LweI GCATC 5 cut(s) 255, 318, 376, 402, 1054
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 2 cut(s) 860, 1160
MalI GATC 9 cut(s) 49, 378, 679, 792, 932, 1083, 1109, 1141, 1242
MbiI CCGCTC 1 cut(s) 97
MboI GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
MboII GAAGA 4 cut(s) 421, 496, 515, 962
MflI RGATCY 1 cut(s) 1139
MluCI AATT 6 cut(s) 352, 456, 592, 877, 939, 1186
MlyI GAGTC 3 cut(s) 237, 881, 970
MmeI TCCRAC 1 cut(s) 363
MroXI GAANNNNTTC 1 cut(s) 741
MseI TTAA 6 cut(s) 258, 392, 765, 942, 981, 1125
MslI CAYNNNNRTG 3 cut(s) 372, 728, 782
MspA1I CMGCKG 3 cut(s) 224, 647, 853
MspI CCGG 3 cut(s) 315, 612, 946
MspR9I CCNGG 2 cut(s) 315, 1275
MssI GTTTAAAC 1 cut(s) 393
Mva1269I GAATGC 3 cut(s) 430, 907, 1095
MvaI CCWGG 1 cut(s) 1275
MvnI CGCG 3 cut(s) 676, 1209, 1302
MwoI GCNNNNNNNGC 9 cut(s) 149, 594, 603, 612, 671, 682, 1101, 1275, 1303
NciI CCSGG 1 cut(s) 315
NdeII GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
NlaIII CATG 6 cut(s) 592, 727, 937, 1104, 1222, 1297
NlaIV GGNNCC 1 cut(s) 1141
NmeAIII GCCGAG 2 cut(s) 454, 1251
NmuCI GTSAC 1 cut(s) 1160
PceI AGGCCT 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 3 cut(s) 430, 907, 1095
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 2 cut(s) 36, 75
PkrI GCNGC 7 cut(s) 20, 469, 646, 772, 1208, 1224, 1252
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 236, 880, 970
PmeI GTTTAAAC 1 cut(s) 393
PpsI GAGTC 3 cut(s) 236, 880, 970
Psp6I CCWGG 1 cut(s) 1273
PspGI CCWGG 1 cut(s) 1273
PspN4I GGNNCC 1 cut(s) 1141
PspPI GGNCC 1 cut(s) 312
PstI CTGCAG 2 cut(s) 646, 775
PstNI CAGNNNCTG 1 cut(s) 927
PsuI RGATCY 1 cut(s) 1139
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 3 cut(s) 224, 647, 853
RseI CAYNNNNRTG 3 cut(s) 372, 728, 782
SalI GTCGAC 1 cut(s) 182
SaqAI TTAA 6 cut(s) 258, 392, 765, 942, 981, 1125
SatI GCNGC 7 cut(s) 19, 468, 645, 771, 1207, 1223, 1251
Sau3AI GATC 9 cut(s) 47, 376, 677, 790, 930, 1081, 1107, 1139, 1240
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 3 cut(s) 237, 881, 970
ScrFI CCNGG 2 cut(s) 315, 1275
SfaNI GCATC 5 cut(s) 255, 318, 376, 402, 1054
SfcI CTRYAG 5 cut(s) 307, 642, 771, 885, 909
SgrDI CGTCGACG 1 cut(s) 182
SmiMI CAYNNNNRTG 3 cut(s) 372, 728, 782
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 6 cut(s) 352, 456, 592, 877, 939, 1186
SseBI AGGCCT 1 cut(s) 798
SspMI CTAG 1 cut(s) 480
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 2 cut(s) 313, 1273
TaaI ACNGT 4 cut(s) 374, 526, 559, 826
TaiI ACGT 1 cut(s) 343
TaqI TCGA 5 cut(s) 50, 73, 183, 233, 245
TasI AATT 6 cut(s) 352, 456, 592, 877, 939, 1186
TauI GCSGC 3 cut(s) 1209, 1225, 1253
TfiI GAWTC 2 cut(s) 36, 75
Tru1I TTAA 6 cut(s) 258, 392, 765, 942, 981, 1125
Tru9I TTAA 6 cut(s) 258, 392, 765, 942, 981, 1125
TscAI CASTG 2 cut(s) 379, 1165
TseFI GTSAC 1 cut(s) 1160
TseI GCWGC 4 cut(s) 18, 467, 644, 770
Tsp45I GTSAC 1 cut(s) 1160
TspDTI ATGAA 4 cut(s) 605, 712, 791, 1207
TspGWI ACGGA 2 cut(s) 23, 169
TspRI CASTG 2 cut(s) 379, 1165
XmiI GTMKAC 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.