FvH4_4g34530

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015306
Physical Location & Seq
Forward (+)
32254077 .. 32256829
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34530.t2

Sequence Viewer

Length: 1329 bp
ATGGCGACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTGCTAAGACCGAACCCTCCCATGCCCTCTCAATTCTTAAACCACTCACCACCGCTTCTCATTCCTCTCGTCTCCTCCCACCGCCTCTCCTTTTCTACTGCCACCGCCGCTTGCTCTCTCCCCGATACTCTCGCCACCGTCGACGACGACGCCTCCTCCACTCCTCCGTTTACTTCTCCGAACAAAAGCTCCAAGGTGCTTCTCAAAGGGATGCGATACCATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACCGGCCCGGGCAAGCTATGATGCTGTGGAAGCGTCTCTACGGGAATGATGTATGGGCGCATCATAGTGATCAGTTGGAAGGTTTGAACAAGGATTTTAGGAAGATGTTGAGTGAGAATGCCGAGTTTAAGGCGTTGTGTTTGAAGGAAATTGTTACCGCGGCGGATGGAACTAGGAAGATTTTATTCACGTTGGAAGATGGGATGGTGATTGAGACCGTTGTTATACCTTCCGATAGTAGGAGGAATACTGTTTGTGTTTCGAGTCAAGTGGGCTGTGGCATGAACTGCCAGTTTTGCTACACTGGCAGGATGGGTCTAAGGAGACATCTGACTGCTGCTGAGATTGTAGAACAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGAAATATTTCCAATGTTGTATTCATGGGAATGGGAGAACCACTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTTAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCCCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTTTAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATTATGCCAATTAACCGAAAGTATAATATAAACCTGCTTCTTCAGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTTTTTGAATATGTAATGCTTGCAGGAGTGAATGACAGCATTGAGGATGCAAGAAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTCATCTCATTTAATCCTCATAGTGGATCCCATTTCAGACCAACCTGTGACGAGAAAATGATCGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGCGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCTTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAGGCTCCCATGCTCCGTGTCCCAGAGAAGTTCAAAATGGCGACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

443

Amino Acids

49.56

Weight (kDa)

8.8

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 228 - 397 3.4e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 957
AccI GTMKAC 1 cut(s) 180
AccII CGCG 3 cut(s) 452, 661, 1194
AclWI GGATC 3 cut(s) 657, 1119, 1132
AcoI YGGCCR 1 cut(s) 1208
AcuI CTGAAG 1 cut(s) 941
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 4 cut(s) 531, 840, 1121, 1217
AgsI TTSAA 8 cut(s) 281, 379, 436, 816, 886, 985, 1010, 1303
AjnI CCWGG 2 cut(s) 1258, 1269
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
AluBI AGCT 8 cut(s) 18, 228, 265, 308, 755, 838, 875, 1249
AluI AGCT 8 cut(s) 18, 228, 265, 308, 755, 838, 875, 1249
Alw26I GTCTC 5 cut(s) 25, 115, 332, 500, 610
AlwI GGATC 3 cut(s) 657, 1119, 1132
AlwNI CAGNNNCTG 1 cut(s) 912
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 4 cut(s) 296, 781, 1189, 1208
ApeKI GCWGC 3 cut(s) 18, 629, 755
Asp700I GAANNNNTTC 2 cut(s) 691, 726
AspLEI GCGC 2 cut(s) 352, 661
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 2 cut(s) 300, 301
AsuHPI GGTGA 2 cut(s) 78, 511
AvaI CYCGRG 1 cut(s) 299
BamHI GGATCC 1 cut(s) 1124
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BbvCI CCTCAGC 1 cut(s) 839
BbvI GCAGC 3 cut(s) 5, 616, 742
BccI CCATC 5 cut(s) 452, 485, 490, 598, 1225
BciT130I CCWGG 2 cut(s) 1260, 1271
BclI TGATCA 3 cut(s) 361, 775, 1225
BcnI CCSGG 2 cut(s) 300, 301
BcoDI GTCTC 5 cut(s) 25, 115, 332, 500, 610
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 2 cut(s) 756, 894
BfuAI ACCTGC 1 cut(s) 957
BisI GCNGC 8 cut(s) 19, 147, 453, 630, 756, 1192, 1208, 1236
BlsI GCNGC 8 cut(s) 20, 148, 454, 631, 757, 1193, 1209, 1237
Bme1390I CCNGG 4 cut(s) 300, 301, 1260, 1271
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 1126, 1275
BmrFI CCNGG 4 cut(s) 300, 301, 1260, 1271
BmsI GCATC 4 cut(s) 240, 303, 361, 1039
BpmI CTGGAG 1 cut(s) 1203
Bpu10I CCTNAGC 1 cut(s) 839
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 300, 301
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 231, 299, 450
BsaXI ACNNNNNCTCC 8 cut(s) 110, 140, 176, 179, 206, 209, 212, 242
Bsc4I CCNNNNNNNGG 4 cut(s) 531, 840, 1121, 1217
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 3 cut(s) 583, 601, 917
BseBI CCWGG 2 cut(s) 1260, 1271
BseDI CCNNGG 3 cut(s) 231, 299, 450
BseGI GGATG 7 cut(s) 33, 255, 463, 501, 609, 778, 1054
BseLI CCNNNNNNNGG 4 cut(s) 531, 840, 1121, 1217
BseMII CTCAG 3 cut(s) 624, 830, 865
BseNI ACTGG 3 cut(s) 583, 601, 917
BseRI GAGGAG 3 cut(s) 103, 184, 192
BseX3I CGGCCG 1 cut(s) 1208
BseXI GCAGC 3 cut(s) 5, 616, 742
BseYI CCCAGC 1 cut(s) 834
Bsh1236I CGCG 3 cut(s) 452, 661, 1194
Bsh1285I CGRYCG 1 cut(s) 1211
BshFI GGCC 4 cut(s) 298, 783, 1191, 1210
BsiEI CGRYCG 1 cut(s) 1211
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 2 cut(s) 295, 300
BslFI GGGAC 2 cut(s) 815, 1274
BslI CCNNNNNNNGG 4 cut(s) 531, 840, 1121, 1217
BsmAI GTCTC 5 cut(s) 25, 115, 332, 500, 610
BsmBI CGTCTC 3 cut(s) 25, 115, 332
BsmFI GGGAC 2 cut(s) 815, 1274
BsmI GAATGC 3 cut(s) 415, 892, 1080
BsnI GGCC 4 cut(s) 298, 783, 1191, 1210
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 299
Bsp143I GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
BspANI GGCC 4 cut(s) 298, 783, 1191, 1210
BspCNI CTCAG 3 cut(s) 625, 831, 864
BspFNI CGCG 3 cut(s) 452, 661, 1194
BspLI GGNNCC 2 cut(s) 1126, 1275
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 957
BspPI GGATC 3 cut(s) 657, 1119, 1132
BspTNI GGTCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 294
BsrI ACTGG 3 cut(s) 583, 601, 917
BssAI RCCGGY 1 cut(s) 294
BssECI CCNNGG 3 cut(s) 231, 299, 450
BssMI GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 2 cut(s) 1260, 1271
Bst4CI ACNGT 4 cut(s) 178, 511, 544, 811
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 2 cut(s) 579, 1086
BstC8I GCNNGC 5 cut(s) 151, 306, 1023, 1062, 1189
BstDEI CTNAG 5 cut(s) 44, 611, 633, 839, 851
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 7 cut(s) 33, 255, 463, 501, 609, 778, 1054
BstFNI CGCG 3 cut(s) 452, 661, 1194
BstHHI GCGC 2 cut(s) 352, 661
BstKTI GATC 8 cut(s) 364, 665, 778, 1069, 1095, 1127, 1161, 1228
BstMAI GTCTC 5 cut(s) 25, 115, 332, 500, 610
BstMBI GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
BstMCI CGRYCG 1 cut(s) 1211
BstMWI GCNNNNNNNGC 9 cut(s) 146, 322, 579, 588, 597, 656, 667, 1086, 1260
BstNI CCWGG 2 cut(s) 1260, 1271
BstSCI CCNGG 4 cut(s) 298, 299, 1258, 1269
BstSFI CTRYAG 2 cut(s) 756, 894
BstUI CGCG 3 cut(s) 452, 661, 1194
BstV1I GCAGC 3 cut(s) 5, 616, 742
BstX2I RGATCY 1 cut(s) 1124
BstYI RGATCY 1 cut(s) 1124
BstZI CGGCCG 1 cut(s) 1208
BsuRI GGCC 4 cut(s) 298, 783, 1191, 1210
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 1236
BtsCI GGATG 7 cut(s) 33, 255, 463, 501, 609, 778, 1054
BtsIMutI CAGTG 1 cut(s) 594
BveI ACCTGC 1 cut(s) 957
Cac8I GCNNGC 5 cut(s) 151, 306, 1023, 1062, 1189
CaiI CAGNNNCTG 1 cut(s) 912
CfoI GCGC 2 cut(s) 352, 661
Cfr10I RCCGGY 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 297
Cfr42I CCGCGG 1 cut(s) 453
Cfr9I CCCGGG 1 cut(s) 299
CseI GACGC 2 cut(s) 197, 314
CviAII CATG 7 cut(s) 61, 260, 574, 709, 1086, 1204, 1279
DdeI CTNAG 5 cut(s) 44, 611, 633, 839, 851
DpnI GATC 8 cut(s) 363, 664, 777, 1068, 1094, 1126, 1160, 1227
DpnII GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
EaeI YGGCCR 1 cut(s) 1208
EagI CGGCCG 1 cut(s) 1208
EciI GGCGGA 1 cut(s) 470
EclXI CGGCCG 1 cut(s) 1208
Eco130I CCWWGG 1 cut(s) 231
Eco147I AGGCCT 1 cut(s) 783
Eco31I GGTCTC 1 cut(s) 500
Eco52I CGGCCG 1 cut(s) 1208
Eco57I CTGAAG 1 cut(s) 941
Eco88I CYCGRG 1 cut(s) 299
EcoRII CCWGG 2 cut(s) 1258, 1269
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 3 cut(s) 25, 115, 332
FaeI CATG 7 cut(s) 64, 263, 577, 712, 1089, 1207, 1282
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 2 cut(s) 815, 1274
FatI CATG 7 cut(s) 60, 259, 573, 708, 1085, 1203, 1278
FbaI TGATCA 3 cut(s) 361, 775, 1225
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 8 cut(s) 19, 147, 453, 630, 756, 1192, 1208, 1236
FokI GGATG 7 cut(s) 40, 262, 470, 508, 616, 785, 1061
Fsp4HI GCNGC 8 cut(s) 19, 147, 453, 630, 756, 1192, 1208, 1236
FspBI CTAG 1 cut(s) 465
GlaI GCGC 2 cut(s) 351, 660
GluI GCNGC 8 cut(s) 19, 147, 453, 630, 756, 1192, 1208, 1236
GsaI CCCAGC 1 cut(s) 838
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 4 cut(s) 298, 783, 1191, 1210
HapII CCGG 2 cut(s) 295, 300
HgaI GACGC 2 cut(s) 197, 314
HhaI GCGC 2 cut(s) 352, 661
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 7 cut(s) 64, 263, 577, 712, 1089, 1207, 1282
Hin6I GCGC 2 cut(s) 350, 659
HinP1I GCGC 2 cut(s) 350, 659
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 4 cut(s) 36, 556, 857, 961
HpaII CCGG 2 cut(s) 295, 300
HphI GGTGA 2 cut(s) 78, 511
Hpy166II GTNNAC 2 cut(s) 181, 210
Hpy188I TCNGA 9 cut(s) 219, 526, 624, 854, 908, 960, 1136, 1169, 1230
Hpy188III TCNNGA 1 cut(s) 985
Hpy8I GTNNAC 2 cut(s) 181, 210
Hpy99I CGWCG 5 cut(s) 10, 182, 185, 188, 191
HpyAV CCTTC 5 cut(s) 365, 430, 531, 794, 1050
HpyCH4III ACNGT 4 cut(s) 178, 511, 544, 811
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 5 cut(s) 758, 890, 1025, 1052, 1089
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 322, 579, 588, 597, 656, 667, 1086, 1260
HpyF3I CTNAG 5 cut(s) 44, 611, 633, 839, 851
HpySE526I ACGT 1 cut(s) 482
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 7 cut(s) 64, 263, 577, 712, 1089, 1207, 1282
HspAI GCGC 2 cut(s) 350, 659
Ksp22I TGATCA 3 cut(s) 361, 775, 1225
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
LmnI GCTCC 4 cut(s) 233, 1184, 1279, 1287
Lsp1109I GCAGC 3 cut(s) 5, 616, 742
LweI GCATC 4 cut(s) 240, 303, 361, 1039
MaeI CTAG 1 cut(s) 465
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 3 cut(s) 445, 845, 1145
MalI GATC 8 cut(s) 363, 664, 777, 1068, 1094, 1126, 1160, 1227
MboI GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
MboII GAAGA 4 cut(s) 406, 481, 500, 947
MflI RGATCY 1 cut(s) 1124
MluCI AATT 5 cut(s) 71, 441, 862, 924, 1171
MlyI GAGTC 3 cut(s) 565, 866, 955
MmeI TCCRAC 3 cut(s) 348, 465, 664
MroXI GAANNNNTTC 2 cut(s) 691, 726
MseI TTAA 6 cut(s) 77, 420, 750, 927, 966, 1110
MslI CAYNNNNRTG 3 cut(s) 357, 713, 767
MspA1I CMGCKG 2 cut(s) 452, 838
MspI CCGG 2 cut(s) 295, 300
MspR9I CCNGG 4 cut(s) 300, 301, 1260, 1271
Mva1269I GAATGC 3 cut(s) 415, 892, 1080
MvaI CCWGG 2 cut(s) 1260, 1271
MvnI CGCG 3 cut(s) 452, 661, 1194
MwoI GCNNNNNNNGC 9 cut(s) 146, 322, 579, 588, 597, 656, 667, 1086, 1260
NciI CCSGG 2 cut(s) 300, 301
NdeII GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
NlaIII CATG 7 cut(s) 64, 263, 577, 712, 1089, 1207, 1282
NlaIV GGNNCC 2 cut(s) 1126, 1275
NmeAIII GCCGAG 2 cut(s) 439, 1236
NmuCI GTSAC 1 cut(s) 1145
PceI AGGCCT 1 cut(s) 783
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 3 cut(s) 415, 892, 1080
PdmI GAANNNNTTC 2 cut(s) 691, 726
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 8 cut(s) 20, 148, 454, 631, 757, 1193, 1209, 1237
PleI GAGTC 3 cut(s) 564, 865, 955
PpsI GAGTC 3 cut(s) 564, 865, 955
Psp6I CCWGG 2 cut(s) 1258, 1269
PspFI CCCAGC 1 cut(s) 834
PspGI CCWGG 2 cut(s) 1258, 1269
PspN4I GGNNCC 2 cut(s) 1126, 1275
PspPI GGNCC 1 cut(s) 297
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 912
PsuI RGATCY 1 cut(s) 1124
PvuII CAGCTG 1 cut(s) 838
RseI CAYNNNNRTG 3 cut(s) 357, 713, 767
SacII CCGCGG 1 cut(s) 453
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 6 cut(s) 77, 420, 750, 927, 966, 1110
SatI GCNGC 8 cut(s) 19, 147, 453, 630, 756, 1192, 1208, 1236
Sau3AI GATC 8 cut(s) 361, 662, 775, 1066, 1092, 1124, 1158, 1225
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 3 cut(s) 565, 866, 955
ScrFI CCNGG 4 cut(s) 300, 301, 1260, 1271
SfaNI GCATC 4 cut(s) 240, 303, 361, 1039
SfcI CTRYAG 2 cut(s) 756, 894
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SgrDI CGTCGACG 1 cut(s) 179
SmaI CCCGGG 1 cut(s) 301
SmiMI CAYNNNNRTG 3 cut(s) 357, 713, 767
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SrfI GCCCGGGC 1 cut(s) 301
Sse9I AATT 5 cut(s) 71, 441, 862, 924, 1171
SseBI AGGCCT 1 cut(s) 783
SspI AATATT 1 cut(s) 691
SspMI CTAG 1 cut(s) 465
StuI AGGCCT 1 cut(s) 783
StyD4I CCNGG 4 cut(s) 298, 299, 1258, 1269
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 4 cut(s) 178, 511, 544, 811
TaiI ACGT 1 cut(s) 485
TaqI TCGA 3 cut(s) 180, 554, 1161
TaqII GACCGA 1 cut(s) 64
TasI AATT 5 cut(s) 71, 441, 862, 924, 1171
TauI GCSGC 5 cut(s) 149, 455, 1194, 1210, 1238
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 6 cut(s) 77, 420, 750, 927, 966, 1110
Tru9I TTAA 6 cut(s) 77, 420, 750, 927, 966, 1110
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 1 cut(s) 1145
TseI GCWGC 3 cut(s) 18, 629, 755
Tsp45I GTSAC 1 cut(s) 1145
TspDTI ATGAA 4 cut(s) 590, 697, 776, 1192
TspGWI ACGGA 3 cut(s) 23, 195, 1274
TspMI CCCGGG 1 cut(s) 299
TspRI CASTG 1 cut(s) 601
XmaI CCCGGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 691, 726
XspI CTAG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.