MD16G1092200.v1.1

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015306
Physical Location & Seq
Reverse (-)
6369412 .. 6372295
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1092200.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGGCTGTATGTGGTTGAGACGAATCATGCTCAGACCAACCTCTCTGCCGCCGCAGAAGCCTCCCATGGCCGCCTTGTTAACCCCTGCCACACTCCAAAACCCCGTCTCCTCTCCCTTTCTGAAACCTTCTACGCCATCTCTCACTCCTCTTGCCTCCTCCCACCGCCGCCTTCTCTCAACAGCCGCGTCCTGTTCTCCTCCACGTCCTCTCGTCTCCGTCGACGAAACTTCCTTCGATTCCGGTGGTGAAAGGAGTGGGATTACGAACCGGAGCTCGAAAGTGCTTCTCAAGGGCATGCGATACGATGAACTTGAAAAATGGGTTCAGTCACATGGGTACAGGCCTGGCCAGGCTCTAATGCTATGGAAACGTATATACGGGAATGGTATTTGGGCGCATCACAGTGATCAACTAGAAGGCTTGAACAAAGATTTTAAGAAGATGTTGAGTGAGAGTGCCGAGTTCAAGGCATTGGCTTTGAAACAAGTTATTACCGCGGCTGATGGAACTAGAAAGATTCTGTTCACTTTGGAAGATGGATTGGTGATAGAAACTGTCGTCATACCTAGCGTCGCTAGCGATAGTCGTAGCAGGAATACTGTTTGCGTTTCGAGTCAAGTGGGCTGTGCCATGAATTGTCAATTCTGCTACACTGGCAGGATGGGTCTAAGGAGGAACCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCACGGTCCTTGTTCACAAGTGAAGTTGGCGACATTACGAATGTTGTATTTATGGGAATGGGAGAACCACTTCAGAACATTGACAATGTTCTTAAAGCTGCAGACATAATGCTACATGATCAAGGCCTATATTTCAGTCCTCGAAAGGTCACAGTTTCAACAAGTGGGCTGGTTCCCCAGATGAGGCGTTTCCTTAAGGAATCTAATTGTGCTTTAGCCGTTAGTTTGAATGCAACGACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAATATAAATTAGACTTGCTTCTTCAGACCCTAAGGGAGGAACTTCGCTTCAAGAGTAACTACAAAGTTCTTTTTGAATATGTGATGCTTGCAGGAATAAATGACAGTGTGGAGGATGCGAAGAGGCTTGCTGATCTCGTTCAGGGAATTCCATGCAAGATCAACCTCCTCTCATTTAACCCTCATAGCGGATCCCAATTCAGACCAACCAGCGAGGAGAAGATGGTTGAGTTTCGGAATCTCTTGGCTGAGTTAGGGTGCCGTGTCTTCTTGCGGTCGAGTAGAGGTGATGATCAGATGGCTGCCTGTGGTCAGCTTGGCAATCCCGATGTAGTCCAAGCTCCCTTGCTTCGCGTGCCCGAGCAATTCAAAATGGCAGTGGAAGTCTCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

463

Amino Acids

51.32

Weight (kDa)

9.09

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 205 - 374 1.1e-15 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1251
AccI GTMKAC 1 cut(s) 222
AccII CGCG 3 cut(s) 188, 500, 1347
AclWI GGATC 3 cut(s) 983, 1179, 1192
AcoI YGGCCR 2 cut(s) 69, 349
AcsI RAATTY 1 cut(s) 1140
AcuI CTGAAG 2 cut(s) 773, 1001
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 900, 1030, 1181
AflII CTTAAG 1 cut(s) 911
AgsI TTSAA 9 cut(s) 317, 427, 469, 484, 876, 946, 1045, 1070, 1363
AjiI CACGTC 1 cut(s) 206
AjnI CCWGG 2 cut(s) 346, 351
AluBI AGCT 5 cut(s) 276, 692, 815, 1309, 1334
AluI AGCT 5 cut(s) 276, 692, 815, 1309, 1334
Alw21I GWGCWC 1 cut(s) 278
Alw26I GTCTC 4 cut(s) 13, 112, 220, 1384
AlwI GGATC 3 cut(s) 983, 1179, 1192
AlwNI CAGNNNCTG 1 cut(s) 972
Ama87I CYCGRG 1 cut(s) 1352
AoxI GGCC 4 cut(s) 69, 344, 349, 841
ApeKI GCWGC 3 cut(s) 689, 815, 1295
ApoI RAATTY 1 cut(s) 1140
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 723
AsuHPI GGTGA 3 cut(s) 260, 559, 1292
AsuNHI GCTAGC 1 cut(s) 578
AvaI CYCGRG 1 cut(s) 1352
AvaII GGWCC 1 cut(s) 723
AxyI CCTNAGG 1 cut(s) 1025
BaeGI GKGCMC 1 cut(s) 1353
BalI TGGCCA 1 cut(s) 351
BamHI GGATCC 1 cut(s) 1184
BanI GGYRCC 1 cut(s) 1251
BanII GRGCYC 1 cut(s) 278
BbsI GAAGAC 1 cut(s) 1252
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 701, 802, 1282
BccI CCATC 6 cut(s) 145, 500, 533, 658, 1210, 1285
BceAI ACGGC 2 cut(s) 920, 1239
BciT130I CCWGG 2 cut(s) 348, 353
BclI TGATCA 3 cut(s) 409, 835, 1285
BcoDI GTCTC 4 cut(s) 13, 112, 220, 1384
BfaI CTAG 4 cut(s) 416, 513, 570, 579
BfmI CTRYAG 2 cut(s) 687, 816
BfrI CTTAAG 1 cut(s) 911
BisI GCNGC 9 cut(s) 50, 53, 72, 169, 186, 501, 690, 816, 1296
BlsI GCNGC 9 cut(s) 51, 54, 73, 170, 187, 502, 691, 817, 1297
Bme1390I CCNGG 2 cut(s) 348, 353
Bme18I GGWCC 1 cut(s) 723
BmeT110I CYCGRG 1 cut(s) 1352
BmgBI CACGTC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 723
BmiI GGNNCC 4 cut(s) 680, 891, 1186, 1253
BmrFI CCNGG 2 cut(s) 348, 353
BmsI GCATC 3 cut(s) 409, 1068, 1099
BmtI GCTAGC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 1252
BpuEI CTTGAG 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 66, 498
BsaWI WCCGGW 3 cut(s) 242, 270, 990
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 3 cut(s) 900, 1030, 1181
Bse1I ACTGG 2 cut(s) 661, 977
Bse21I CCTNAGG 1 cut(s) 1025
BseBI CCWGG 2 cut(s) 348, 353
BseDI CCNNGG 2 cut(s) 66, 498
BseGI GGATG 2 cut(s) 669, 1114
BseLI CCNNNNNNNGG 3 cut(s) 900, 1030, 1181
BseMII CTCAG 3 cut(s) 46, 684, 1233
BseNI ACTGG 2 cut(s) 661, 977
BseRI GAGGAG 6 cut(s) 100, 138, 148, 189, 1151, 1223
BseSI GKGCMC 1 cut(s) 1353
BseXI GCAGC 3 cut(s) 701, 802, 1282
Bsh1236I CGCG 3 cut(s) 188, 500, 1347
Bsh1285I CGRYCG 1 cut(s) 1271
BshFI GGCC 4 cut(s) 71, 346, 351, 843
BshNI GGYRCC 1 cut(s) 1251
BsiEI CGRYCG 1 cut(s) 1271
BsiHKAI GWGCWC 1 cut(s) 278
BsiHKCI CYCGRG 1 cut(s) 1352
BsiSI CCGG 3 cut(s) 243, 271, 991
BslI CCNNNNNNNGG 3 cut(s) 900, 1030, 1181
BsmAI GTCTC 4 cut(s) 13, 112, 220, 1384
BsmBI CGTCTC 3 cut(s) 13, 112, 220
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 4 cut(s) 71, 346, 351, 843
BsoBI CYCGRG 1 cut(s) 1352
Bsp1286I GDGCHC 2 cut(s) 278, 1353
Bsp143I GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
Bsp19I CCATGG 1 cut(s) 66
BspANI GGCC 4 cut(s) 71, 346, 351, 843
BspCNI CTCAG 3 cut(s) 45, 685, 1234
BspFNI CGCG 3 cut(s) 188, 500, 1347
BspLI GGNNCC 4 cut(s) 680, 891, 1186, 1253
BspMAI CTGCAG 2 cut(s) 691, 820
BspOI GCTAGC 1 cut(s) 582
BspPI GGATC 3 cut(s) 983, 1179, 1192
BspT107I GGYRCC 1 cut(s) 1251
BspTI CTTAAG 1 cut(s) 911
BsrI ACTGG 2 cut(s) 661, 977
BssECI CCNNGG 2 cut(s) 66, 498
BssMI GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 348, 353
Bst4CI ACNGT 6 cut(s) 407, 559, 604, 723, 871, 1100
Bst6I CTCTTC 1 cut(s) 1109
BstAFI CTTAAG 1 cut(s) 911
BstC8I GCNNGC 6 cut(s) 299, 580, 708, 1083, 1122, 1349
BstDEI CTNAG 5 cut(s) 32, 671, 693, 1025, 1242
BstDSI CCRYGG 2 cut(s) 66, 498
BstENI CCTNNNNNAGG 1 cut(s) 1028
BstF5I GGATG 2 cut(s) 669, 1114
BstFNI CGCG 3 cut(s) 188, 500, 1347
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 7 cut(s) 412, 838, 978, 1129, 1155, 1187, 1288
BstMAI GTCTC 4 cut(s) 13, 112, 220, 1384
BstMBI GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
BstMCI CGRYCG 1 cut(s) 1271
BstMWI GCNNNNNNNGC 5 cut(s) 58, 579, 657, 716, 1348
BstNI CCWGG 2 cut(s) 348, 353
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 2 cut(s) 346, 351
BstSFI CTRYAG 2 cut(s) 687, 816
BstSLI GKGCMC 1 cut(s) 1353
BstUI CGCG 3 cut(s) 188, 500, 1347
BstV1I GCAGC 3 cut(s) 701, 802, 1282
BstV2I GAAGAC 1 cut(s) 1252
BstX2I RGATCY 1 cut(s) 1184
BstYI RGATCY 1 cut(s) 1184
Bsu36I CCTNAGG 1 cut(s) 1025
BsuRI GGCC 4 cut(s) 71, 346, 351, 843
BtgI CCRYGG 2 cut(s) 66, 498
BtrI CACGTC 1 cut(s) 206
BtsCI GGATG 2 cut(s) 669, 1114
BtsI GCAGTG 1 cut(s) 1377
BtsIMutI CAGTG 4 cut(s) 412, 654, 1105, 1377
Cac8I GCNNGC 6 cut(s) 299, 580, 708, 1083, 1122, 1349
CaiI CAGNNNCTG 1 cut(s) 972
CfoI GCGC 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 723
Cfr42I CCGCGG 1 cut(s) 501
CseI GACGC 2 cut(s) 177, 562
Csp6I GTAC 1 cut(s) 340
CviAII CATG 8 cut(s) 28, 67, 298, 335, 634, 833, 979, 1146
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 5 cut(s) 32, 671, 693, 1025, 1242
DpnI GATC 7 cut(s) 411, 837, 977, 1128, 1154, 1186, 1287
DpnII GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
EaeI YGGCCR 2 cut(s) 69, 349
Eam1104I CTCTTC 1 cut(s) 1109
EarI CTCTTC 1 cut(s) 1109
Ecl136II GAGCTC 1 cut(s) 276
Eco130I CCWWGG 1 cut(s) 66
Eco147I AGGCCT 2 cut(s) 346, 843
Eco24I GRGCYC 1 cut(s) 278
Eco47I GGWCC 1 cut(s) 723
Eco53kI GAGCTC 1 cut(s) 276
Eco57I CTGAAG 2 cut(s) 773, 1001
Eco81I CCTNAGG 1 cut(s) 1025
Eco88I CYCGRG 1 cut(s) 1352
EcoICRI GAGCTC 1 cut(s) 276
EcoNI CCTNNNNNAGG 1 cut(s) 1028
EcoRI GAATTC 1 cut(s) 1140
EcoRII CCWGG 2 cut(s) 346, 351
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 278
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 3 cut(s) 13, 112, 220
FaeI CATG 8 cut(s) 31, 70, 301, 338, 637, 836, 982, 1149
FatI CATG 8 cut(s) 27, 66, 297, 334, 633, 832, 978, 1145
FbaI TGATCA 3 cut(s) 409, 835, 1285
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 9 cut(s) 50, 53, 72, 169, 186, 501, 690, 816, 1296
FokI GGATG 2 cut(s) 676, 1121
FriOI GRGCYC 1 cut(s) 278
Fsp4HI GCNGC 9 cut(s) 50, 53, 72, 169, 186, 501, 690, 816, 1296
FspBI CTAG 4 cut(s) 416, 513, 570, 579
GlaI GCGC 1 cut(s) 399
GluI GCNGC 9 cut(s) 50, 53, 72, 169, 186, 501, 690, 816, 1296
HaeIII GGCC 4 cut(s) 71, 346, 351, 843
HapII CCGG 3 cut(s) 243, 271, 991
HgaI GACGC 2 cut(s) 177, 562
HhaI GCGC 1 cut(s) 400
Hin1II CATG 8 cut(s) 31, 70, 301, 338, 637, 836, 982, 1149
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 2 cut(s) 81, 223
HindII GTYRAC 2 cut(s) 81, 223
HinfI GANTC 6 cut(s) 24, 239, 520, 616, 917, 1231
HpaI GTTAAC 1 cut(s) 81
HpaII CCGG 3 cut(s) 243, 271, 991
HphI GGTGA 3 cut(s) 260, 559, 1292
Hpy166II GTNNAC 4 cut(s) 81, 223, 528, 732
Hpy188I TCNGA 8 cut(s) 35, 123, 792, 968, 1020, 1196, 1230, 1290
Hpy188III TCNNGA 2 cut(s) 1045, 1319
Hpy8I GTNNAC 4 cut(s) 81, 223, 528, 732
Hpy99I CGWCG 3 cut(s) 224, 227, 578
HpyAV CCTTC 4 cut(s) 138, 182, 244, 413
HpyCH4III ACNGT 6 cut(s) 407, 559, 604, 723, 871, 1100
HpyCH4IV ACGT 2 cut(s) 205, 373
HpyCH4V TGCA 6 cut(s) 689, 719, 818, 950, 1085, 1149
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 579, 657, 716, 1348
HpyF3I CTNAG 5 cut(s) 32, 671, 693, 1025, 1242
HpySE526I ACGT 2 cut(s) 205, 373
Hsp92II CATG 8 cut(s) 31, 70, 301, 338, 637, 836, 982, 1149
HspAI GCGC 1 cut(s) 398
Ksp22I TGATCA 3 cut(s) 409, 835, 1285
KspAI GTTAAC 1 cut(s) 81
KspI CCGCGG 1 cut(s) 501
Kzo9I GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
LmnI GCTCC 2 cut(s) 273, 1339
Lsp1109I GCAGC 3 cut(s) 701, 802, 1282
LweI GCATC 3 cut(s) 409, 1068, 1099
MaeI CTAG 4 cut(s) 416, 513, 570, 579
MaeII ACGT 2 cut(s) 205, 373
MaeIII GTNAC 3 cut(s) 330, 865, 1049
MalI GATC 7 cut(s) 411, 837, 977, 1128, 1154, 1186, 1287
MboI GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
MboII GAAGA 6 cut(s) 454, 548, 1007, 1126, 1225, 1252
MflI RGATCY 1 cut(s) 1184
MhlI GDGCHC 2 cut(s) 278, 1353
MlsI TGGCCA 1 cut(s) 351
MluCI AATT 8 cut(s) 637, 644, 922, 984, 1001, 1140, 1190, 1358
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 1 cut(s) 625
Mox20I TGGCCA 1 cut(s) 351
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 351
MseI TTAA 6 cut(s) 80, 438, 810, 912, 987, 1170
MslI CAYNNNNRTG 1 cut(s) 405
Msp20I TGGCCA 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 500, 692
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 3 cut(s) 243, 271, 991
MspR9I CCNGG 2 cut(s) 348, 353
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 2 cut(s) 348, 353
MvnI CGCG 3 cut(s) 188, 500, 1347
MwoI GCNNNNNNNGC 5 cut(s) 58, 579, 657, 716, 1348
NcoI CCATGG 1 cut(s) 66
NdeII GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 8 cut(s) 31, 70, 301, 338, 637, 836, 982, 1149
NlaIV GGNNCC 4 cut(s) 680, 891, 1186, 1253
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 2 cut(s) 330, 865
NspI RCATGY 1 cut(s) 301
PaeI GCATGC 1 cut(s) 301
PceI AGGCCT 2 cut(s) 346, 843
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 5 cut(s) 24, 239, 520, 917, 1231
PkrI GCNGC 9 cut(s) 51, 54, 73, 170, 187, 502, 691, 817, 1297
PleI GAGTC 1 cut(s) 624
PpsI GAGTC 1 cut(s) 624
Psp124BI GAGCTC 1 cut(s) 278
Psp6I CCWGG 2 cut(s) 346, 351
PspGI CCWGG 2 cut(s) 346, 351
PspN4I GGNNCC 4 cut(s) 680, 891, 1186, 1253
PspPI GGNCC 1 cut(s) 723
PstI CTGCAG 2 cut(s) 691, 820
PstNI CAGNNNCTG 1 cut(s) 972
PsuI RGATCY 1 cut(s) 1184
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 405
SacI GAGCTC 1 cut(s) 278
SacII CCGCGG 1 cut(s) 501
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 6 cut(s) 80, 438, 810, 912, 987, 1170
SatI GCNGC 9 cut(s) 50, 53, 72, 169, 186, 501, 690, 816, 1296
Sau3AI GATC 7 cut(s) 409, 835, 975, 1126, 1152, 1184, 1285
Sau96I GGNCC 1 cut(s) 723
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 2 cut(s) 348, 353
SduI GDGCHC 2 cut(s) 278, 1353
SfaNI GCATC 3 cut(s) 409, 1068, 1099
SfcI CTRYAG 2 cut(s) 687, 816
Sfr303I CCGCGG 1 cut(s) 501
SgrBI CCGCGG 1 cut(s) 501
SgrDI CGTCGACG 1 cut(s) 221
SinI GGWCC 1 cut(s) 723
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 2 cut(s) 290, 911
SmoI CTYRAG 2 cut(s) 290, 911
SphI GCATGC 1 cut(s) 301
Sse9I AATT 8 cut(s) 637, 644, 922, 984, 1001, 1140, 1190, 1358
SseBI AGGCCT 2 cut(s) 346, 843
SspMI CTAG 4 cut(s) 416, 513, 570, 579
SstI GAGCTC 1 cut(s) 278
StuI AGGCCT 2 cut(s) 346, 843
StyD4I CCNGG 2 cut(s) 346, 351
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 6 cut(s) 407, 559, 604, 723, 871, 1100
TaiI ACGT 2 cut(s) 208, 376
TaqI TCGA 6 cut(s) 222, 237, 278, 614, 859, 1271
TasI AATT 8 cut(s) 637, 644, 922, 984, 1001, 1140, 1190, 1358
TauI GCSGC 6 cut(s) 52, 55, 74, 171, 188, 503
TfiI GAWTC 5 cut(s) 24, 239, 520, 917, 1231
Tru1I TTAA 6 cut(s) 80, 438, 810, 912, 987, 1170
Tru9I TTAA 6 cut(s) 80, 438, 810, 912, 987, 1170
TscAI CASTG 4 cut(s) 412, 661, 1105, 1377
TseFI GTSAC 2 cut(s) 330, 865
TseI GCWGC 3 cut(s) 689, 815, 1295
Tsp45I GTSAC 2 cut(s) 330, 865
TspDTI ATGAA 2 cut(s) 324, 650
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 4 cut(s) 412, 661, 1105, 1377
Vha464I CTTAAG 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 723
XagI CCTNNNNNAGG 1 cut(s) 1028
XapI RAATTY 1 cut(s) 1140
XceI RCATGY 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 4 cut(s) 416, 513, 570, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.