Rroxscaffold_5G00384450

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015306
Physical Location & Seq
Forward (+)
63827462 .. 63839241
11780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384450.1

Sequence Viewer

Length: 1527 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTTCGACCGAACCCTCTACCTCTCCACAAGGCTCCCACGCCCTCTCCTCTCTCCTCTCGATTCCTGAAACACTCACCACCGCTCCTCATTCCTCTCGTTTCGTCCCCCCACCGCCTCTCGTTTTCCACAGCCACCGCCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTCCTCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAAGCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACAGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATTATATTTGGGCGCATCACAGTGATCAGTTGGCAGGTTTAAACAAAGATTTTAGGAAGATGTTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTGAAGCAAATTATTACCGCAGCGGATGGAACTAGGAAGGGCATATATGGGAGTTTTAGGATGGGTCTAAGGAGACATCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAGGTGAAGTTGGCGAGATTACCAATGTTGTATTCATGGGAATGGGAGAACCGCTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTTTTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTTATCTCATTTAATCCTCATAGTGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGCGTTGTTTTCATGCGGCCAAGTAGAGGCGATGATCAGATGGCAGCCTGTGGTCAGCTTGGCAAACCTGGTGAAATCCAGGCTCCTATGCTCCGCGTGCCAGAGAAGTTCAGAACGGCGACCCAAGTGGTGAAGGAAAATAATCTCAGTGGTCAACCAAAACAGTCACATTATTTGGAGGTGAGGGAAAAGAATATTTATATGGTTTCTTTACATAGGTACAGCTGGAGGTGCAGGAAAGCAGGGGTAGGTTCAAGCTCATCAATTCTGTACGTGAATTCAACCAGAAATAAATTTGATTTCTTTCTCTCTACACAGGATTTCTGGGTGGCTCACACAGGTTCTCTGGGAGAATTACACATAAATATTGCTTGGAAGATGCATAATTGCACAGTCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

508

Amino Acids

57.17

Weight (kDa)

9.61

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 182 - 332 2.7e-09 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 407
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 213
AccII CGCG 3 cut(s) 595, 1128, 1221
AclWI GGATC 4 cut(s) 591, 857, 1053, 1066
AcoI YGGCCR 2 cut(s) 346, 1142
AcsI RAATTY 2 cut(s) 1402, 1418
AfaI GTAC 2 cut(s) 1346, 1397
AfiI CCNNNNNNNGG 2 cut(s) 1055, 1151
AgsI TTSAA 9 cut(s) 326, 466, 481, 750, 820, 919, 944, 1380, 1407
AjnI CCWGG 2 cut(s) 1192, 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
Alw26I GTCTC 4 cut(s) 25, 265, 377, 544
AlwI GGATC 4 cut(s) 591, 857, 1053, 1066
AlwNI CAGNNNCTG 1 cut(s) 846
AoxI GGCC 5 cut(s) 341, 346, 715, 1123, 1142
ApeKI GCWGC 5 cut(s) 18, 497, 563, 689, 1169
ApoI RAATTY 2 cut(s) 1402, 1418
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 2 cut(s) 397, 595
AspS9I GGNCC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 6 cut(s) 108, 248, 623, 1208, 1267, 1318
BamHI GGATCC 1 cut(s) 1058
BbvCI CCTCAGC 2 cut(s) 768, 773
BbvI GCAGC 5 cut(s) 5, 509, 575, 676, 1181
BccI CCATC 3 cut(s) 497, 532, 1159
BceAI ACGGC 2 cut(s) 866, 1257
BciT130I CCWGG 2 cut(s) 1194, 1205
BclI TGATCA 3 cut(s) 406, 709, 1159
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 4 cut(s) 25, 265, 377, 544
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 5 cut(s) 337, 561, 690, 804, 828
BfuAI ACCTGC 1 cut(s) 407
BisI GCNGC 8 cut(s) 19, 180, 498, 564, 690, 1126, 1142, 1170
BlsI GCNGC 8 cut(s) 20, 181, 499, 565, 691, 1127, 1143, 1171
Bme1390I CCNGG 3 cut(s) 345, 1194, 1205
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 3 cut(s) 75, 1060, 1209
BmrFI CCNGG 3 cut(s) 345, 1194, 1205
BmsI GCATC 5 cut(s) 285, 348, 406, 973, 1494
BpmI CTGGAG 3 cut(s) 276, 1137, 1372
Bpu10I CCTNAGC 2 cut(s) 768, 773
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 345
BsaAI YACGTR 1 cut(s) 1399
BsaWI WCCGGW 1 cut(s) 864
BsaXI ACNNNNNCTCC 6 cut(s) 70, 100, 108, 138, 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 1055, 1151
Bse1I ACTGG 2 cut(s) 851, 1077
BseBI CCWGG 2 cut(s) 1194, 1205
BseGI GGATG 6 cut(s) 33, 300, 508, 543, 712, 988
BseLI CCNNNNNNNGG 2 cut(s) 1055, 1151
BseMII CTCAG 5 cut(s) 558, 764, 782, 799, 1285
BseNI ACTGG 2 cut(s) 851, 1077
BseRI GAGGAG 3 cut(s) 78, 85, 116
BseXI GCAGC 5 cut(s) 5, 509, 575, 676, 1181
BsgI GTGCAG 1 cut(s) 1378
Bsh1236I CGCG 3 cut(s) 595, 1128, 1221
Bsh1285I CGRYCG 1 cut(s) 50
BshFI GGCC 5 cut(s) 343, 348, 717, 1125, 1144
BsiEI CGRYCG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 345, 865
BslFI GGGAC 2 cut(s) 130, 749
BslI CCNNNNNNNGG 2 cut(s) 1055, 1151
BsmAI GTCTC 4 cut(s) 25, 265, 377, 544
BsmBI CGTCTC 2 cut(s) 25, 377
BsmFI GGGAC 2 cut(s) 130, 749
BsmI GAATGC 3 cut(s) 460, 826, 1014
BsnI GGCC 5 cut(s) 343, 348, 717, 1125, 1144
Bsp143I GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
BspANI GGCC 5 cut(s) 343, 348, 717, 1125, 1144
BspCNI CTCAG 5 cut(s) 559, 765, 781, 798, 1284
BspFNI CGCG 3 cut(s) 595, 1128, 1221
BspLI GGNNCC 3 cut(s) 75, 1060, 1209
BspMAI CTGCAG 2 cut(s) 565, 694
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 4 cut(s) 591, 857, 1053, 1066
BsrBI CCGCTC 1 cut(s) 124
BsrI ACTGG 2 cut(s) 851, 1077
BssMI GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
Bst2UI CCWGG 2 cut(s) 1194, 1205
Bst4CI ACNGT 4 cut(s) 404, 745, 1290, 1519
BstAPI GCANNNNNTGC 1 cut(s) 1020
BstBAI YACGTR 1 cut(s) 1399
BstC8I GCNNGC 5 cut(s) 582, 957, 996, 1123, 1223
BstDEI CTNAG 6 cut(s) 545, 567, 768, 773, 785, 1271
BstF5I GGATG 6 cut(s) 33, 300, 508, 543, 712, 988
BstFNI CGCG 3 cut(s) 595, 1128, 1221
BstHHI GCGC 2 cut(s) 397, 595
BstKTI GATC 8 cut(s) 409, 599, 712, 852, 1003, 1029, 1061, 1162
BstMAI GTCTC 4 cut(s) 25, 265, 377, 544
BstMBI GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 6 cut(s) 179, 590, 601, 1020, 1222, 1356
BstNI CCWGG 2 cut(s) 1194, 1205
BstSCI CCNGG 3 cut(s) 343, 1192, 1203
BstSFI CTRYAG 5 cut(s) 337, 561, 690, 804, 828
BstUI CGCG 3 cut(s) 595, 1128, 1221
BstV1I GCAGC 5 cut(s) 5, 509, 575, 676, 1181
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
BsuRI GGCC 5 cut(s) 343, 348, 717, 1125, 1144
BtgZI GCGATG 1 cut(s) 1170
BtsCI GGATG 6 cut(s) 33, 300, 508, 543, 712, 988
BtsIMutI CAGTG 3 cut(s) 409, 1084, 1279
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 5 cut(s) 582, 957, 996, 1123, 1223
CaiI CAGNNNCTG 1 cut(s) 846
CfoI GCGC 2 cut(s) 397, 595
Cfr13I GGNCC 1 cut(s) 342
CsiI ACCWGGT 1 cut(s) 1192
Csp6I GTAC 2 cut(s) 1345, 1396
CviAII CATG 4 cut(s) 643, 853, 1020, 1138
CviQI GTAC 2 cut(s) 1345, 1396
DdeI CTNAG 6 cut(s) 545, 567, 768, 773, 785, 1271
DpnI GATC 8 cut(s) 408, 598, 711, 851, 1002, 1028, 1060, 1161
DpnII GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
DraI TTTAAA 1 cut(s) 423
EaeI YGGCCR 2 cut(s) 346, 1142
Eco147I AGGCCT 1 cut(s) 717
EcoRI GAATTC 1 cut(s) 1402
EcoRII CCWGG 2 cut(s) 1192, 1203
EcoT22I ATGCAT 1 cut(s) 1509
Esp3I CGTCTC 2 cut(s) 25, 377
FaeI CATG 4 cut(s) 646, 856, 1023, 1141
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 2 cut(s) 130, 749
FatI CATG 4 cut(s) 642, 852, 1019, 1137
FbaI TGATCA 3 cut(s) 406, 709, 1159
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 8 cut(s) 19, 180, 498, 564, 690, 1126, 1142, 1170
FokI GGATG 6 cut(s) 40, 307, 515, 550, 719, 995
Fsp4HI GCNGC 8 cut(s) 19, 180, 498, 564, 690, 1126, 1142, 1170
FspBI CTAG 1 cut(s) 510
GlaI GCGC 2 cut(s) 396, 594
GluI GCNGC 8 cut(s) 19, 180, 498, 564, 690, 1126, 1142, 1170
GsuI CTGGAG 3 cut(s) 276, 1137, 1372
HaeIII GGCC 5 cut(s) 343, 348, 717, 1125, 1144
HapII CCGG 2 cut(s) 345, 865
HhaI GCGC 2 cut(s) 397, 595
Hin1II CATG 4 cut(s) 646, 856, 1023, 1141
Hin6I GCGC 2 cut(s) 395, 593
HinP1I GCGC 2 cut(s) 395, 593
HincII GTYRAC 2 cut(s) 214, 1280
HindII GTYRAC 2 cut(s) 214, 1280
HinfI GANTC 5 cut(s) 36, 102, 258, 791, 895
HpaII CCGG 2 cut(s) 345, 865
HphI GGTGA 6 cut(s) 108, 248, 623, 1208, 1267, 1318
Hpy166II GTNNAC 3 cut(s) 214, 249, 1280
Hpy188I TCNGA 8 cut(s) 558, 788, 842, 894, 1070, 1103, 1164, 1238
Hpy188III TCNNGA 4 cut(s) 99, 106, 262, 919
Hpy8I GTNNAC 3 cut(s) 214, 249, 1280
Hpy99I CGWCG 2 cut(s) 215, 218
HpyAV CCTTC 5 cut(s) 252, 271, 508, 728, 1252
HpyCH4III ACNGT 4 cut(s) 404, 745, 1290, 1519
HpyCH4IV ACGT 2 cut(s) 370, 1398
HpyCH4V TGCA 9 cut(s) 563, 692, 824, 959, 986, 1023, 1359, 1507, 1515
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 590, 601, 1020, 1222, 1356
HpyF3I CTNAG 6 cut(s) 545, 567, 768, 773, 785, 1271
HpySE526I ACGT 2 cut(s) 370, 1398
Hsp92II CATG 4 cut(s) 646, 856, 1023, 1141
HspAI GCGC 2 cut(s) 395, 593
Ksp22I TGATCA 3 cut(s) 406, 709, 1159
Kzo9I GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
LmnI GCTCC 5 cut(s) 79, 129, 1118, 1213, 1221
Lsp1109I GCAGC 5 cut(s) 5, 509, 575, 676, 1181
LweI GCATC 5 cut(s) 285, 348, 406, 973, 1494
MabI ACCWGGT 1 cut(s) 1192
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 370, 1398
MaeIII GTNAC 3 cut(s) 779, 1079, 1290
MalI GATC 8 cut(s) 408, 598, 711, 851, 1002, 1028, 1060, 1161
MbiI CCGCTC 1 cut(s) 124
MboI GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
MboII GAAGA 3 cut(s) 451, 881, 1513
MflI RGATCY 1 cut(s) 1058
MlyI GAGTC 3 cut(s) 267, 800, 889
Mph1103I ATGCAT 1 cut(s) 1509
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 7 cut(s) 288, 422, 684, 861, 900, 1044, 1525
MslI CAYNNNNRTG 3 cut(s) 402, 647, 701
MspA1I CMGCKG 5 cut(s) 254, 500, 566, 772, 1350
MspI CCGG 2 cut(s) 345, 865
MspR9I CCNGG 3 cut(s) 345, 1194, 1205
MssI GTTTAAAC 1 cut(s) 423
Mva1269I GAATGC 3 cut(s) 460, 826, 1014
MvaI CCWGG 2 cut(s) 1194, 1205
MvnI CGCG 3 cut(s) 595, 1128, 1221
MwoI GCNNNNNNNGC 6 cut(s) 179, 590, 601, 1020, 1222, 1356
NciI CCSGG 1 cut(s) 345
NdeII GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
NlaIII CATG 4 cut(s) 646, 856, 1023, 1141
NlaIV GGNNCC 3 cut(s) 75, 1060, 1209
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 1079, 1290
NsiI ATGCAT 1 cut(s) 1509
PceI AGGCCT 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 3 cut(s) 460, 826, 1014
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 2 cut(s) 36, 102
PkrI GCNGC 8 cut(s) 20, 181, 499, 565, 691, 1127, 1143, 1171
PleI GAGTC 3 cut(s) 266, 799, 889
PmeI GTTTAAAC 1 cut(s) 423
PpsI GAGTC 3 cut(s) 266, 799, 889
Ppu21I YACGTR 1 cut(s) 1399
Psp6I CCWGG 2 cut(s) 1192, 1203
PspGI CCWGG 2 cut(s) 1192, 1203
PspN4I GGNNCC 3 cut(s) 75, 1060, 1209
PspPI GGNCC 1 cut(s) 342
PstI CTGCAG 2 cut(s) 565, 694
PstNI CAGNNNCTG 1 cut(s) 846
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 4 cut(s) 254, 566, 772, 1350
RsaI GTAC 2 cut(s) 1346, 1397
RsaNI GTAC 2 cut(s) 1345, 1396
RseI CAYNNNNRTG 3 cut(s) 402, 647, 701
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 7 cut(s) 288, 422, 684, 861, 900, 1044, 1525
SatI GCNGC 8 cut(s) 19, 180, 498, 564, 690, 1126, 1142, 1170
Sau3AI GATC 8 cut(s) 406, 596, 709, 849, 1000, 1026, 1058, 1159
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 3 cut(s) 267, 800, 889
ScrFI CCNGG 3 cut(s) 345, 1194, 1205
SexAI ACCWGGT 1 cut(s) 1192
SfaNI GCATC 5 cut(s) 285, 348, 406, 973, 1494
SfcI CTRYAG 5 cut(s) 337, 561, 690, 804, 828
SgrDI CGTCGACG 1 cut(s) 212
SmiMI CAYNNNNRTG 3 cut(s) 402, 647, 701
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SseBI AGGCCT 1 cut(s) 717
SspI AATATT 2 cut(s) 1321, 1492
SspMI CTAG 1 cut(s) 510
StuI AGGCCT 1 cut(s) 717
StyD4I CCNGG 3 cut(s) 343, 1192, 1203
TaaI ACNGT 4 cut(s) 404, 745, 1290, 1519
TaiI ACGT 2 cut(s) 373, 1401
TaqI TCGA 5 cut(s) 46, 100, 213, 263, 275
TaqII GACCGA 1 cut(s) 64
TauI GCSGC 3 cut(s) 182, 1128, 1144
TfiI GAWTC 2 cut(s) 36, 102
Tru1I TTAA 7 cut(s) 288, 422, 684, 861, 900, 1044, 1525
Tru9I TTAA 7 cut(s) 288, 422, 684, 861, 900, 1044, 1525
TscAI CASTG 3 cut(s) 409, 1084, 1279
TseFI GTSAC 2 cut(s) 1079, 1290
TseI GCWGC 5 cut(s) 18, 497, 563, 689, 1169
Tsp45I GTSAC 2 cut(s) 1079, 1290
TspDTI ATGAA 3 cut(s) 631, 710, 1126
TspGWI ACGGA 2 cut(s) 23, 199
TspRI CASTG 3 cut(s) 409, 1084, 1279
XapI RAATTY 2 cut(s) 1402, 1418
XmiI GTMKAC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 660
XspI CTAG 1 cut(s) 510
Zsp2I ATGCAT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.