RLG00000005878

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015306
Physical Location & Seq
Reverse (-)
2515325 .. 2517663
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005878

Sequence Viewer

Length: 1371 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTCCGATCGAACCCTCTACCTCTCCACAAGGCTCCCACGCCCTCTCCTCTCTCCTCTCAATTCCCAAAACACTCACCACCACTCCTCATTCCTCTCGTTTCGTCCCACCGCCTCTCCTTTTCTACAGCTACCGCCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTCCTCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAATCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACAGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATTATATTTGGGCGCATCACAGTGATCAGTTGGAAGGTTTAAACAAAGATTTTAGGAAGATGTTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTGAAGCAAATTATTACCGCAGCGGATGGAACTAGGAAGATTTTATTCACTTTGGAAGATGGGATGGTGATTGAAACCGTTGTCATACCTTCTGATAGTAGCAGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGGCATGAATTGCCAGTTCTGCTACACTGGCAGGATGGGTCTAAGGAGACATCTGACTGCAGCGGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGCGAGATTACCAATGTTGTATTCATGGGAATGGGAGAACCGCTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTTTTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTTATCTCATTTAATCCTCATAGCGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGTGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCCTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAGGCTCCCATGCTCCGCGTGCCAGAGAAGTTCAAAACGGCGACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

457

Amino Acids

50.82

Weight (kDa)

9.04

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 199 - 368 2.7e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 210
AccII CGCG 2 cut(s) 703, 1329
AclWI GGATC 4 cut(s) 699, 965, 1161, 1174
AcoI YGGCCR 2 cut(s) 343, 1250
AfiI CCNNNNNNNGG 2 cut(s) 1163, 1259
AjnI CCWGG 2 cut(s) 1300, 1311
AjuI GAANNNNNNNTTGG 2 cut(s) 731, 763
AluBI AGCT 9 cut(s) 18, 170, 251, 307, 350, 797, 880, 917, 1291
AluI AGCT 9 cut(s) 18, 170, 251, 307, 350, 797, 880, 917, 1291
Alw26I GTCTC 4 cut(s) 25, 262, 374, 652
AlwI GGATC 4 cut(s) 699, 965, 1161, 1174
AlwNI CAGNNNCTG 1 cut(s) 954
AoxI GGCC 6 cut(s) 338, 343, 823, 1231, 1250, 1278
ApeKI GCWGC 4 cut(s) 18, 494, 671, 797
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 2 cut(s) 394, 703
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 3 cut(s) 108, 245, 553
BamHI GGATCC 1 cut(s) 1166
BbvCI CCTCAGC 2 cut(s) 876, 881
BbvI GCAGC 4 cut(s) 5, 506, 683, 784
BccI CCATC 5 cut(s) 494, 527, 532, 640, 1267
BceAI ACGGC 3 cut(s) 974, 1218, 1365
BciT130I CCWGG 2 cut(s) 1302, 1313
BclI TGATCA 3 cut(s) 403, 817, 1267
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 4 cut(s) 25, 262, 374, 652
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 6 cut(s) 165, 334, 669, 798, 912, 936
BisI GCNGC 7 cut(s) 19, 177, 495, 672, 798, 1250, 1278
BlsI GCNGC 7 cut(s) 20, 178, 496, 673, 799, 1251, 1279
Bme1390I CCNGG 3 cut(s) 342, 1302, 1313
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 3 cut(s) 75, 1168, 1317
BmrFI CCNGG 3 cut(s) 342, 1302, 1313
BmsI GCATC 4 cut(s) 282, 345, 403, 1081
BpmI CTGGAG 2 cut(s) 273, 1245
Bpu10I CCTNAGC 2 cut(s) 876, 881
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 972
BsaXI ACNNNNNCTCC 8 cut(s) 70, 100, 108, 138, 140, 170, 206, 236
Bsc4I CCNNNNNNNGG 2 cut(s) 1163, 1259
Bse1I ACTGG 4 cut(s) 625, 643, 959, 1185
BseBI CCWGG 2 cut(s) 1302, 1313
BseGI GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BseLI CCNNNNNNNGG 2 cut(s) 1163, 1259
BseMII CTCAG 3 cut(s) 872, 890, 907
BseNI ACTGG 4 cut(s) 625, 643, 959, 1185
BseRI GAGGAG 3 cut(s) 78, 85, 116
BseX3I CGGCCG 1 cut(s) 1250
BseXI GCAGC 4 cut(s) 5, 506, 683, 784
Bsh1236I CGCG 2 cut(s) 703, 1329
Bsh1285I CGRYCG 2 cut(s) 50, 1253
BshFI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BsiEI CGRYCG 2 cut(s) 50, 1253
BsiSI CCGG 2 cut(s) 342, 973
BslFI GGGAC 2 cut(s) 130, 857
BslI CCNNNNNNNGG 2 cut(s) 1163, 1259
BsmAI GTCTC 4 cut(s) 25, 262, 374, 652
BsmBI CGTCTC 2 cut(s) 25, 374
BsmFI GGGAC 2 cut(s) 130, 857
BsmI GAATGC 3 cut(s) 457, 934, 1122
BsnI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
Bsp143I GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
BspANI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BspCNI CTCAG 3 cut(s) 873, 889, 906
BspFNI CGCG 2 cut(s) 703, 1329
BspLI GGNNCC 3 cut(s) 75, 1168, 1317
BspMAI CTGCAG 2 cut(s) 673, 802
BspPI GGATC 4 cut(s) 699, 965, 1161, 1174
BsrI ACTGG 4 cut(s) 625, 643, 959, 1185
BssMI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
Bst2UI CCWGG 2 cut(s) 1302, 1313
Bst4CI ACNGT 4 cut(s) 401, 553, 586, 853
BstAPI GCANNNNNTGC 2 cut(s) 621, 1128
BstC8I GCNNGC 5 cut(s) 690, 1065, 1104, 1231, 1331
BstDEI CTNAG 4 cut(s) 653, 876, 881, 893
BstF5I GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BstFNI CGCG 2 cut(s) 703, 1329
BstHHI GCGC 2 cut(s) 394, 703
BstKTI GATC 9 cut(s) 50, 406, 707, 820, 960, 1111, 1137, 1169, 1270
BstMAI GTCTC 4 cut(s) 25, 262, 374, 652
BstMBI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
BstMCI CGRYCG 2 cut(s) 50, 1253
BstMWI GCNNNNNNNGC 9 cut(s) 176, 621, 630, 639, 698, 709, 1128, 1302, 1330
BstNI CCWGG 2 cut(s) 1302, 1313
BstSCI CCNGG 3 cut(s) 340, 1300, 1311
BstSFI CTRYAG 6 cut(s) 165, 334, 669, 798, 912, 936
BstUI CGCG 2 cut(s) 703, 1329
BstV1I GCAGC 4 cut(s) 5, 506, 683, 784
BstX2I RGATCY 1 cut(s) 1166
BstYI RGATCY 1 cut(s) 1166
BstZI CGGCCG 1 cut(s) 1250
BsuRI GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
BtgZI GCGATG 1 cut(s) 1278
BtsCI GGATG 7 cut(s) 33, 297, 505, 543, 651, 820, 1096
BtsIMutI CAGTG 3 cut(s) 406, 636, 1192
Cac8I GCNNGC 5 cut(s) 690, 1065, 1104, 1231, 1331
CaiI CAGNNNCTG 1 cut(s) 954
CfoI GCGC 2 cut(s) 394, 703
Cfr13I GGNCC 1 cut(s) 339
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviAII CATG 6 cut(s) 616, 751, 961, 1128, 1246, 1321
DdeI CTNAG 4 cut(s) 653, 876, 881, 893
DpnI GATC 9 cut(s) 49, 405, 706, 819, 959, 1110, 1136, 1168, 1269
DpnII GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
DraI TTTAAA 1 cut(s) 420
EaeI YGGCCR 2 cut(s) 343, 1250
EagI CGGCCG 1 cut(s) 1250
EclXI CGGCCG 1 cut(s) 1250
Eco147I AGGCCT 1 cut(s) 825
Eco52I CGGCCG 1 cut(s) 1250
EcoRII CCWGG 2 cut(s) 1300, 1311
Esp3I CGTCTC 2 cut(s) 25, 374
FaeI CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 2 cut(s) 130, 857
FatI CATG 6 cut(s) 615, 750, 960, 1127, 1245, 1320
FbaI TGATCA 3 cut(s) 403, 817, 1267
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 7 cut(s) 19, 177, 495, 672, 798, 1250, 1278
FokI GGATG 7 cut(s) 40, 304, 512, 550, 658, 827, 1103
Fsp4HI GCNGC 7 cut(s) 19, 177, 495, 672, 798, 1250, 1278
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 393, 702
GluI GCNGC 7 cut(s) 19, 177, 495, 672, 798, 1250, 1278
GsuI CTGGAG 2 cut(s) 273, 1245
HaeIII GGCC 6 cut(s) 340, 345, 825, 1233, 1252, 1280
HapII CCGG 2 cut(s) 342, 973
HhaI GCGC 2 cut(s) 394, 703
Hin1II CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
Hin6I GCGC 2 cut(s) 392, 701
HinP1I GCGC 2 cut(s) 392, 701
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 4 cut(s) 36, 255, 899, 1003
HpaII CCGG 2 cut(s) 342, 973
HphI GGTGA 3 cut(s) 108, 245, 553
Hpy166II GTNNAC 2 cut(s) 211, 246
Hpy188I TCNGA 9 cut(s) 47, 568, 666, 896, 950, 1002, 1178, 1211, 1272
Hpy188III TCNNGA 3 cut(s) 259, 271, 1027
Hpy8I GTNNAC 2 cut(s) 211, 246
Hpy99I CGWCG 2 cut(s) 212, 215
HpyAV CCTTC 5 cut(s) 249, 268, 407, 573, 836
HpyCH4III ACNGT 4 cut(s) 401, 553, 586, 853
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 6 cut(s) 671, 800, 932, 1067, 1094, 1131
HpyF10VI GCNNNNNNNGC 9 cut(s) 176, 621, 630, 639, 698, 709, 1128, 1302, 1330
HpyF3I CTNAG 4 cut(s) 653, 876, 881, 893
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
HspAI GCGC 2 cut(s) 392, 701
Ksp22I TGATCA 3 cut(s) 403, 817, 1267
Kzo9I GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
LmnI GCTCC 4 cut(s) 79, 1226, 1321, 1329
Lsp1109I GCAGC 4 cut(s) 5, 506, 683, 784
LweI GCATC 4 cut(s) 282, 345, 403, 1081
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 2 cut(s) 887, 1187
MalI GATC 9 cut(s) 49, 405, 706, 819, 959, 1110, 1136, 1168, 1269
MboI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
MboII GAAGA 4 cut(s) 448, 523, 542, 989
MflI RGATCY 1 cut(s) 1166
MluCI AATT 7 cut(s) 101, 379, 483, 619, 904, 966, 1213
MlyI GAGTC 3 cut(s) 264, 908, 997
MmeI TCCRAC 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
MslI CAYNNNNRTG 3 cut(s) 399, 755, 809
MspA1I CMGCKG 4 cut(s) 251, 497, 674, 880
MspI CCGG 2 cut(s) 342, 973
MspR9I CCNGG 3 cut(s) 342, 1302, 1313
MssI GTTTAAAC 1 cut(s) 420
Mva1269I GAATGC 3 cut(s) 457, 934, 1122
MvaI CCWGG 2 cut(s) 1302, 1313
MvnI CGCG 2 cut(s) 703, 1329
MwoI GCNNNNNNNGC 9 cut(s) 176, 621, 630, 639, 698, 709, 1128, 1302, 1330
NciI CCSGG 1 cut(s) 342
NdeII GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
NlaIII CATG 6 cut(s) 619, 754, 964, 1131, 1249, 1324
NlaIV GGNNCC 3 cut(s) 75, 1168, 1317
NmeAIII GCCGAG 2 cut(s) 481, 1278
NmuCI GTSAC 1 cut(s) 1187
PceI AGGCCT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 3 cut(s) 457, 934, 1122
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 7 cut(s) 20, 178, 496, 673, 799, 1251, 1279
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 263, 907, 997
PmeI GTTTAAAC 1 cut(s) 420
PpsI GAGTC 3 cut(s) 263, 907, 997
Psp6I CCWGG 2 cut(s) 1300, 1311
PspGI CCWGG 2 cut(s) 1300, 1311
PspN4I GGNNCC 3 cut(s) 75, 1168, 1317
PspPI GGNCC 1 cut(s) 339
PstI CTGCAG 2 cut(s) 673, 802
PstNI CAGNNNCTG 1 cut(s) 954
PsuI RGATCY 1 cut(s) 1166
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 2 cut(s) 251, 880
RseI CAYNNNNRTG 3 cut(s) 399, 755, 809
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
SatI GCNGC 7 cut(s) 19, 177, 495, 672, 798, 1250, 1278
Sau3AI GATC 9 cut(s) 47, 403, 704, 817, 957, 1108, 1134, 1166, 1267
Sau96I GGNCC 1 cut(s) 339
SchI GAGTC 3 cut(s) 264, 908, 997
ScrFI CCNGG 3 cut(s) 342, 1302, 1313
SfaNI GCATC 4 cut(s) 282, 345, 403, 1081
SfcI CTRYAG 6 cut(s) 165, 334, 669, 798, 912, 936
SgrDI CGTCGACG 1 cut(s) 209
SmiMI CAYNNNNRTG 3 cut(s) 399, 755, 809
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 7 cut(s) 101, 379, 483, 619, 904, 966, 1213
SseBI AGGCCT 1 cut(s) 825
SspMI CTAG 1 cut(s) 507
StuI AGGCCT 1 cut(s) 825
StyD4I CCNGG 3 cut(s) 340, 1300, 1311
TaaI ACNGT 4 cut(s) 401, 553, 586, 853
TaiI ACGT 1 cut(s) 370
TaqI TCGA 4 cut(s) 50, 210, 260, 272
TasI AATT 7 cut(s) 101, 379, 483, 619, 904, 966, 1213
TauI GCSGC 3 cut(s) 179, 1252, 1280
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
Tru9I TTAA 6 cut(s) 285, 419, 792, 969, 1008, 1152
TscAI CASTG 3 cut(s) 406, 643, 1192
TseFI GTSAC 1 cut(s) 1187
TseI GCWGC 4 cut(s) 18, 494, 671, 797
Tsp45I GTSAC 1 cut(s) 1187
TspDTI ATGAA 4 cut(s) 632, 739, 818, 1234
TspGWI ACGGA 2 cut(s) 23, 196
TspRI CASTG 3 cut(s) 406, 643, 1192
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.