Prupe.1G254200_v2.0.a1
ERF Family

Radical SAM superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015306
Physical Location & Seq
Forward (+)
26495180 .. 26498294
3115 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G254200.1

Sequence Viewer

Length: 1380 bp
ATGGGTCGTATGTGGATGAGACGGATCCTACTCAGACAAAACTCTCTACCTCTGCTGAAGCCTCCCATGGCCTGCTTGTTAACGCCTGCGACACTCCAAAAGCCCATCTCCTCTCCCTTTCTGAAACCATCACCACCAACTCTAGCTCCTCTTGCCTCGTCCCACCGTCGCTCTCTTTCAACAGCAACAGCTTGTTCTCTTCCGGATCCTCTCGTTTCCCTCGATGGAATTTCTTCCTATTTTGGAGGAGATGACGGCGTTGGAACTTCAAACAAGGGCTTGAAGGTTCTTCTCAAGGGCATGACATACGATGAGCTTGAAAAATGGGTTCAGTCTCATGGCTACAGGCCTGGCCAGGCTTTAATGCTATGGAAACGTCTCTATGGGAACGGTATTTGGGCGCATCATAGTGATCAGCTGGAAGGCTTGAATAAAGATTTTAAGAAGATGTTGAGTGAGAATGCTGAATTCAAGGCATTGTCTTTGAAACAAGTTATTACCGCCGCTGATGGAACTAGGAAGATTTTGTTCACTTTGGAAGATGGATTGGTGATAGAAACTGTTGTCATACCTTCCGATAGTAGCAGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGCCATGAATTGTCAGTTCTGCTACACTGGCAGGATGGGTTTAAGAAGACATCTGACTGCTGCCGAGATTGTGGAGCAGGCTGTCTTTGCACGGTGCCTGCTTACAAATGAAGTTGGTGACATTACAAATGTTGTATTTATGGGAATGGGAGAACCACTTCAGAACATTGACAATGTTCTTAAAGCTACAGACATAATGCTGCATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATCACTGTTTCAACAAGTGGGCTTGTCCCCCAGCTGAGGCGTTTCCTCAAAGCATCTAATTGTGCTTTAGCTGTTAGTTTGAATGCAACGACTGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAATATAACATAGGCTTGCTTCTTCAGACCCTTAGGGAGGAACTTCGCTTCAAGAATAACTACAAAGTTCTTTTTGAATATGTAATGCTTGCAGGAGTTAATGATAGCATTGAGGATGCAAGGAGGCTTGCTGATCTTGTTCGGGGAATTCCATGCAAGATCAATCTTCTCTCATTTAATCCTCATGGCGGATCCCAATTCAGACCAACCAGTGACGAGAAGATGATTGAGTTTCGAAATCTTTTGGCTGAAGCAGGGTGTGTTGTCTTCTTGCGGTCGAGTAGAGGTGATGATCAGATGGCTGCTTGTGGTCAGCTTGGAAATCCTGGTGCAATCCAGGCTCCCTTACTCCGTGTGCCAGAGCAATTCAAAATGGCAATGGAAGTGTCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 Radical_SAM

Protein Analysis

460

Amino Acids

50.81

Weight (kDa)

9.05

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 714
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 4 cut(s) 501, 504, 1173, 1258
AclWI GGATC 7 cut(s) 19, 32, 200, 213, 974, 1170, 1183
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 3 cut(s) 228, 467, 1131
AcuI CTGAAG 4 cut(s) 77, 764, 992, 1254
AfiI CCNNNNNNNGG 3 cut(s) 891, 1021, 1172
AjnI CCWGG 4 cut(s) 349, 354, 1309, 1320
AluBI AGCT 8 cut(s) 146, 191, 316, 418, 806, 889, 926, 1300
AluI AGCT 8 cut(s) 146, 191, 316, 418, 806, 889, 926, 1300
Alw26I GTCTC 3 cut(s) 13, 339, 383
AlwI GGATC 7 cut(s) 19, 32, 200, 213, 974, 1170, 1183
AlwNI CAGNNNCTG 1 cut(s) 963
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 4 cut(s) 69, 347, 352, 832
ApeKI GCWGC 3 cut(s) 680, 820, 1286
ApoI RAATTY 3 cut(s) 228, 467, 1131
Asp700I GAANNNNTTC 2 cut(s) 232, 777
AspLEI GCGC 1 cut(s) 403
AsuHPI GGTGA 4 cut(s) 123, 562, 749, 1283
AsuII TTCGAA 1 cut(s) 1219
AxyI CCTNAGG 1 cut(s) 1016
BalI TGGCCA 1 cut(s) 354
BamHI GGATCC 3 cut(s) 24, 205, 1175
BanI GGYRCC 1 cut(s) 714
BbsI GAAGAC 2 cut(s) 673, 1243
BbvCI CCTCAGC 1 cut(s) 890
BbvI GCAGC 3 cut(s) 667, 807, 1273
BccI CCATC 7 cut(s) 113, 136, 218, 503, 536, 649, 1276
BceAI ACGGC 1 cut(s) 271
BciT130I CCWGG 4 cut(s) 351, 356, 1311, 1322
BclI TGATCA 3 cut(s) 412, 826, 1276
BcoDI GTCTC 3 cut(s) 13, 339, 383
BfaI CTAG 2 cut(s) 143, 516
BfmI CTRYAG 2 cut(s) 343, 807
BisI GCNGC 4 cut(s) 504, 681, 821, 1287
BlsI GCNGC 4 cut(s) 505, 682, 822, 1288
Bme1390I CCNGG 4 cut(s) 351, 356, 1311, 1322
BmiI GGNNCC 5 cut(s) 26, 207, 716, 1177, 1326
BmrFI CCNGG 4 cut(s) 351, 356, 1311, 1322
BmsI GCATC 3 cut(s) 412, 917, 1090
BpiI GAAGAC 2 cut(s) 673, 1243
Bpu10I CCTNAGC 1 cut(s) 890
Bpu14I TTCGAA 1 cut(s) 1219
BpuEI CTTGAG 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 2 cut(s) 202, 981
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 3 cut(s) 891, 1021, 1172
Bse1I ACTGG 3 cut(s) 652, 968, 1194
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 1 cut(s) 1368
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 4 cut(s) 351, 356, 1311, 1322
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 3 cut(s) 21, 660, 1105
BseLI CCNNNNNNNGG 3 cut(s) 891, 1021, 1172
BseMI GCAATG 1 cut(s) 1368
BseMII CTCAG 2 cut(s) 46, 881
BseNI ACTGG 3 cut(s) 652, 968, 1194
BseRI GAGGAG 3 cut(s) 100, 138, 261
BseXI GCAGC 3 cut(s) 667, 807, 1273
BseYI CCCAGC 1 cut(s) 885
Bsh1285I CGRYCG 1 cut(s) 1262
BshFI GGCC 4 cut(s) 71, 349, 354, 834
BshNI GGYRCC 1 cut(s) 714
BsiEI CGRYCG 1 cut(s) 1262
BsiSI CCGG 2 cut(s) 203, 982
BslFI GGGAC 2 cut(s) 145, 866
BslI CCNNNNNNNGG 3 cut(s) 891, 1021, 1172
BsmAI GTCTC 3 cut(s) 13, 339, 383
BsmBI CGTCTC 2 cut(s) 13, 383
BsmFI GGGAC 2 cut(s) 145, 866
BsmI GAATGC 2 cut(s) 466, 943
BsnI GGCC 4 cut(s) 71, 349, 354, 834
Bsp119I TTCGAA 1 cut(s) 1219
Bsp13I TCCGGA 1 cut(s) 202
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 4 cut(s) 501, 504, 1173, 1258
BspANI GGCC 4 cut(s) 71, 349, 354, 834
BspCNI CTCAG 2 cut(s) 45, 882
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 5 cut(s) 26, 207, 716, 1177, 1326
BspPI GGATC 7 cut(s) 19, 32, 200, 213, 974, 1170, 1183
BspT104I TTCGAA 1 cut(s) 1219
BspT107I GGYRCC 1 cut(s) 714
BsrDI GCAATG 1 cut(s) 1368
BsrI ACTGG 3 cut(s) 652, 968, 1194
BssECI CCNNGG 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 4 cut(s) 351, 356, 1311, 1322
Bst4CI ACNGT 6 cut(s) 167, 392, 562, 595, 714, 862
Bst6I CTCTTC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 1219
BstC8I GCNNGC 7 cut(s) 73, 87, 699, 719, 1001, 1074, 1113
BstDEI CTNAG 3 cut(s) 32, 890, 1016
BstDSI CCRYGG 1 cut(s) 66
BstENI CCTNNNNNAGG 1 cut(s) 1019
BstF5I GGATG 3 cut(s) 21, 660, 1105
BstHHI GCGC 1 cut(s) 403
BstMAI GTCTC 3 cut(s) 13, 339, 383
BstMCI CGRYCG 1 cut(s) 1262
BstMWI GCNNNNNNNGC 4 cut(s) 152, 648, 707, 1322
BstNI CCWGG 4 cut(s) 351, 356, 1311, 1322
BstSCI CCNGG 4 cut(s) 349, 354, 1309, 1320
BstSFI CTRYAG 2 cut(s) 343, 807
BstV1I GCAGC 3 cut(s) 667, 807, 1273
BstV2I GAAGAC 2 cut(s) 673, 1243
BstX2I RGATCY 3 cut(s) 24, 205, 1175
BstYI RGATCY 3 cut(s) 24, 205, 1175
Bsu36I CCTNAGG 1 cut(s) 1016
BsuRI GGCC 4 cut(s) 71, 349, 354, 834
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 3 cut(s) 21, 660, 1105
BtsIMutI CAGTG 3 cut(s) 645, 858, 1201
Cac8I GCNNGC 7 cut(s) 73, 87, 699, 719, 1001, 1074, 1113
CaiI CAGNNNCTG 1 cut(s) 963
CfoI GCGC 1 cut(s) 403
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 8 cut(s) 67, 301, 338, 625, 824, 970, 1137, 1169
DdeI CTNAG 3 cut(s) 32, 890, 1016
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 1188
Eco130I CCWWGG 1 cut(s) 66
Eco147I AGGCCT 2 cut(s) 349, 834
Eco57I CTGAAG 4 cut(s) 77, 764, 992, 1254
Eco81I CCTNAGG 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 1019
EcoRI GAATTC 2 cut(s) 467, 1131
EcoRII CCWGG 4 cut(s) 349, 354, 1309, 1320
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 13, 383
FaeI CATG 8 cut(s) 70, 304, 341, 628, 827, 973, 1140, 1172
FaqI GGGAC 2 cut(s) 145, 866
FatI CATG 8 cut(s) 66, 300, 337, 624, 823, 969, 1136, 1168
FbaI TGATCA 3 cut(s) 412, 826, 1276
Fnu4HI GCNGC 4 cut(s) 504, 681, 821, 1287
FokI GGATG 3 cut(s) 28, 667, 1112
Fsp4HI GCNGC 4 cut(s) 504, 681, 821, 1287
FspBI CTAG 2 cut(s) 143, 516
GlaI GCGC 1 cut(s) 402
GluI GCNGC 4 cut(s) 504, 681, 821, 1287
GsaI CCCAGC 1 cut(s) 889
HaeIII GGCC 4 cut(s) 71, 349, 354, 834
HapII CCGG 2 cut(s) 203, 982
HhaI GCGC 1 cut(s) 403
Hin1II CATG 8 cut(s) 70, 304, 341, 628, 827, 973, 1140, 1172
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HpaI GTTAAC 1 cut(s) 81
HpaII CCGG 2 cut(s) 203, 982
HphI GGTGA 4 cut(s) 123, 562, 749, 1283
Hpy166II GTNNAC 2 cut(s) 81, 531
Hpy188I TCNGA 9 cut(s) 35, 123, 577, 675, 783, 959, 1011, 1187, 1281
Hpy188III TCNNGA 2 cut(s) 203, 1036
Hpy8I GTNNAC 2 cut(s) 81, 531
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 4 cut(s) 277, 416, 582, 845
HpyCH4III ACNGT 6 cut(s) 167, 392, 562, 595, 714, 862
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 7 cut(s) 710, 823, 941, 1076, 1103, 1140, 1316
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 648, 707, 1322
HpyF3I CTNAG 3 cut(s) 32, 890, 1016
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 8 cut(s) 70, 304, 341, 628, 827, 973, 1140, 1172
HspAI GCGC 1 cut(s) 401
Kpn2I TCCGGA 1 cut(s) 202
Ksp22I TGATCA 3 cut(s) 412, 826, 1276
KspAI GTTAAC 1 cut(s) 81
LmnI GCTCC 3 cut(s) 151, 694, 1330
Lsp1109I GCAGC 3 cut(s) 667, 807, 1273
LweI GCATC 3 cut(s) 412, 917, 1090
MaeI CTAG 2 cut(s) 143, 516
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 2 cut(s) 737, 1196
MflI RGATCY 3 cut(s) 24, 205, 1175
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 8 cut(s) 228, 467, 628, 913, 975, 1131, 1181, 1349
MluNI TGGCCA 1 cut(s) 354
MmeI TCCRAC 1 cut(s) 241
Mox20I TGGCCA 1 cut(s) 354
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 232, 777
MscI TGGCCA 1 cut(s) 354
MseI TTAA 8 cut(s) 80, 362, 441, 662, 801, 978, 1083, 1161
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 3 cut(s) 418, 506, 889
MspI CCGG 2 cut(s) 203, 982
MspR9I CCNGG 4 cut(s) 351, 356, 1311, 1322
Mva1269I GAATGC 2 cut(s) 466, 943
MvaI CCWGG 4 cut(s) 351, 356, 1311, 1322
MwoI GCNNNNNNNGC 4 cut(s) 152, 648, 707, 1322
NcoI CCATGG 1 cut(s) 66
NlaIII CATG 8 cut(s) 70, 304, 341, 628, 827, 973, 1140, 1172
NlaIV GGNNCC 5 cut(s) 26, 207, 716, 1177, 1326
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 737, 1196
NspV TTCGAA 1 cut(s) 1219
PceI AGGCCT 2 cut(s) 349, 834
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 2 cut(s) 466, 943
PdmI GAANNNNTTC 2 cut(s) 232, 777
PkrI GCNGC 4 cut(s) 505, 682, 822, 1288
Psp6I CCWGG 4 cut(s) 349, 354, 1309, 1320
PspFI CCCAGC 1 cut(s) 885
PspGI CCWGG 4 cut(s) 349, 354, 1309, 1320
PspN4I GGNNCC 5 cut(s) 26, 207, 716, 1177, 1326
PstNI CAGNNNCTG 1 cut(s) 963
PsuI RGATCY 3 cut(s) 24, 205, 1175
PvuII CAGCTG 2 cut(s) 418, 889
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 8 cut(s) 80, 362, 441, 662, 801, 978, 1083, 1161
SatI GCNGC 4 cut(s) 504, 681, 821, 1287
ScrFI CCNGG 4 cut(s) 351, 356, 1311, 1322
SfaNI GCATC 3 cut(s) 412, 917, 1090
SfcI CTRYAG 2 cut(s) 343, 807
SfuI TTCGAA 1 cut(s) 1219
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 8 cut(s) 228, 467, 628, 913, 975, 1131, 1181, 1349
SseBI AGGCCT 2 cut(s) 349, 834
SsiI CCGC 4 cut(s) 501, 504, 1173, 1258
SspMI CTAG 2 cut(s) 143, 516
StuI AGGCCT 2 cut(s) 349, 834
StyD4I CCNGG 4 cut(s) 349, 354, 1309, 1320
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 6 cut(s) 167, 392, 562, 595, 714, 862
TaiI ACGT 1 cut(s) 379
TaqI TCGA 3 cut(s) 222, 1219, 1262
TasI AATT 8 cut(s) 228, 467, 628, 913, 975, 1131, 1181, 1349
TauI GCSGC 1 cut(s) 506
Tru1I TTAA 8 cut(s) 80, 362, 441, 662, 801, 978, 1083, 1161
Tru9I TTAA 8 cut(s) 80, 362, 441, 662, 801, 978, 1083, 1161
TscAI CASTG 3 cut(s) 652, 865, 1201
TseFI GTSAC 2 cut(s) 737, 1196
TseI GCWGC 3 cut(s) 680, 820, 1286
Tsp45I GTSAC 2 cut(s) 737, 1196
TspDTI ATGAA 3 cut(s) 641, 744, 827
TspGWI ACGGA 2 cut(s) 37, 1325
TspRI CASTG 3 cut(s) 652, 865, 1201
XagI CCTNNNNNAGG 1 cut(s) 1019
XapI RAATTY 3 cut(s) 228, 467, 1131
XmnI GAANNNNTTC 2 cut(s) 232, 777
XspI CTAG 2 cut(s) 143, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.