Rh4BG419800

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015306
Physical Location & Seq
Forward (+)
56585192 .. 56587549
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG419800.1

Sequence Viewer

Length: 1347 bp
ATGGCAACGGAGATGAGCTGCGTGTGGATGAGACGAATCCTACTCCGATCGAACCCTCTACCTCTCTCCTCTCGATTCCTGAAACACTCACCACCACCGCTCCTCATTCCTCTCGTTTCGTCCCACCGCCTCTCGTTTTCCACAGCCACCACCGCTTGTTCTCTCCCAGACACTCTCGCCTCCGTCGACGACACTTGCCCCGCTTTTGGTGAGCCTTCGTTTACAGCTGGAGTCTCGAACAAAACCTCGAAGGTGCTTCTTAAAGGGATGCGATACGATGAGCTTGAGAAATGGGTTCAATCACAAGGCTACAGGCCCGGCCAAGCTATGATGCTATGGAAACGTCTCTATGGGAATTATGTTTGGGCGCATCACAGTGATCAGTTGGAAGGTTTAAACAAAGATTTTAGGAAGATGTTGAGTGAGAATGCCGAGTTCAAGGCATTGTCTTTCAAGCAAATTATTACCGCAGCGGATGGAACTAGGAAGATTTTATTCACTTTGGAAGATGGGATGGTGATTGAAACCGTTGTCATACCTTCTGATAGTAGCAGGAATACTGTTTGTGTTTCAAGTCAAGTGGGCTGTGGCATGAATTGCCAGTTCTGCTACACCGGCAGGATGGGTCTAAGGAGACATCTGACTGCAGCTGAGATTGTAGAGCAGGCTGTATTTGCGCGATCCCTGCTCACAAGTGAAGTTGGCGAGATTACCAATGTTGTATTCATGGGAATGGGAGAACCGCTTCACAACATTGACAATGTTATTAAAGCTGCAGACATTATGGTGGATGATCAAGGCCTTCATTTCAGTCCTCGCAAGATAACTGTTTCAACAAGTGGGCTTGTCCCTCAGCTGAGGCGTTACCTCAGAGAGTCTAATTGTGCTATAGCTGTTAGTTTGAATGCAACTACAGATGAGGTCAGAAACTGGATCATGCCAATTAACCGGAAGTATAATATAGCCGTGCTTCTTCGGACTCTTAAAGAGGAACTTCACTTCAAGAATAACTACAAAGTTCTATTTGAATATGTAATGCTTGCAGGAGTAAATGACAGCATTGAGGATGCAAGGAGGCTTGCTGATCTCGTTCAGGGCATTCCATGCAAGATCAACCTTATCTCATTTAATCCTCATAGCGGATCCCATTTCAGACCAACCAGTGACGAGAAAATGGTTGAGTTCAGAAATTATTTGGCTGGAGCAGGCCGTGTTGTTTTCATGCGGCCGAGTAGAGGCGATGATCAGATGGCGGCCTGTGGTCAGCTTGGCAAACCTGGCGAAATCCAAGCTCCCATGCTCCGCGTGCCAGAGAAGTTCAAAACGGCAACCCAAGTGGTAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

448

Amino Acids

50.08

Weight (kDa)

8.92

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Radical_SAM PF04055 191 - 360 3.5e-16 Radical SAM superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60230
fragaria_vesca FvH4_4g34530 FvH4_4g34530
malus_domestica MD16G1092200.v1.1
prunus_persica Prupe.1G254200_v2.0.a1
pyrus_communis pycom16g07860
rosa_chinensis RchiOBHm_Chr4g0443901
rosa_laevigata RLG00000005878
rosa_multiflora Rmu_sc0019057.1_g000001
rosa_roxburghii Rroxscaffold_5G00384450
rosa_rugosa Rorug04G0348200
rosa_samantha Rh4AG408700 Rh4BG419800 Rh4CG433800 Rh4DG414800
rosa_wichuraiana Rw4G035100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 679, 1305
AclWI GGATC 4 cut(s) 675, 941, 1137, 1150
AcoI YGGCCR 2 cut(s) 319, 1226
AfiI CCNNNNNNNGG 3 cut(s) 206, 1139, 1235
AjnI CCWGG 1 cut(s) 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
Alw26I GTCTC 4 cut(s) 25, 238, 350, 628
AlwI GGATC 4 cut(s) 675, 941, 1137, 1150
AlwNI CAGNNNCTG 1 cut(s) 930
AoxI GGCC 6 cut(s) 314, 319, 799, 1207, 1226, 1254
ApeKI GCWGC 4 cut(s) 18, 470, 647, 773
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 2 cut(s) 370, 679
AspS9I GGNCC 1 cut(s) 315
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 81, 221, 529
BamHI GGATCC 1 cut(s) 1142
BbvCI CCTCAGC 2 cut(s) 852, 857
BbvI GCAGC 4 cut(s) 5, 482, 659, 760
BccI CCATC 5 cut(s) 470, 503, 508, 616, 1243
BceAI ACGGC 3 cut(s) 950, 1194, 1341
BciT130I CCWGG 1 cut(s) 1278
BclI TGATCA 3 cut(s) 379, 793, 1243
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 4 cut(s) 25, 238, 350, 628
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 5 cut(s) 310, 645, 774, 888, 912
BisI GCNGC 6 cut(s) 19, 471, 648, 774, 1226, 1254
BlsI GCNGC 6 cut(s) 20, 472, 649, 775, 1227, 1255
Bme1390I CCNGG 2 cut(s) 318, 1278
BmgT120I GGNCC 1 cut(s) 315
BmiI GGNNCC 1 cut(s) 1144
BmrFI CCNGG 2 cut(s) 318, 1278
BmsI GCATC 4 cut(s) 258, 321, 379, 1057
BpmI CTGGAG 2 cut(s) 249, 1221
Bpu10I CCTNAGC 2 cut(s) 852, 857
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 318
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 1139, 1235
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 3 cut(s) 601, 935, 1161
BseBI CCWGG 1 cut(s) 1278
BseGI GGATG 7 cut(s) 33, 273, 481, 519, 627, 796, 1072
BseLI CCNNNNNNNGG 3 cut(s) 206, 1139, 1235
BseMII CTCAG 4 cut(s) 642, 848, 866, 883
BseNI ACTGG 3 cut(s) 601, 935, 1161
BseRI GAGGAG 2 cut(s) 58, 92
BseX3I CGGCCG 1 cut(s) 1226
BseXI GCAGC 4 cut(s) 5, 482, 659, 760
Bsh1236I CGCG 2 cut(s) 679, 1305
Bsh1285I CGRYCG 2 cut(s) 50, 1229
BshFI GGCC 6 cut(s) 316, 321, 801, 1209, 1228, 1256
BsiEI CGRYCG 2 cut(s) 50, 1229
BsiSI CCGG 3 cut(s) 318, 615, 949
BslFI GGGAC 2 cut(s) 106, 833
BslI CCNNNNNNNGG 3 cut(s) 206, 1139, 1235
BsmAI GTCTC 4 cut(s) 25, 238, 350, 628
BsmBI CGTCTC 2 cut(s) 25, 350
BsmFI GGGAC 2 cut(s) 106, 833
BsmI GAATGC 3 cut(s) 433, 910, 1098
BsnI GGCC 6 cut(s) 316, 321, 801, 1209, 1228, 1256
Bsp143I GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
BspANI GGCC 6 cut(s) 316, 321, 801, 1209, 1228, 1256
BspCNI CTCAG 4 cut(s) 643, 849, 865, 882
BspFNI CGCG 2 cut(s) 679, 1305
BspLI GGNNCC 1 cut(s) 1144
BspMAI CTGCAG 2 cut(s) 649, 778
BspPI GGATC 4 cut(s) 675, 941, 1137, 1150
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 614
BsrI ACTGG 3 cut(s) 601, 935, 1161
BssAI RCCGGY 1 cut(s) 614
BssMI GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
Bst2UI CCWGG 1 cut(s) 1278
Bst4CI ACNGT 4 cut(s) 377, 529, 562, 829
BstAPI GCANNNNNTGC 2 cut(s) 597, 1104
BstC8I GCNNGC 5 cut(s) 666, 1041, 1080, 1207, 1307
BstDEI CTNAG 5 cut(s) 629, 651, 852, 857, 869
BstF5I GGATG 7 cut(s) 33, 273, 481, 519, 627, 796, 1072
BstFNI CGCG 2 cut(s) 679, 1305
BstHHI GCGC 2 cut(s) 370, 679
BstKTI GATC 9 cut(s) 50, 382, 683, 796, 936, 1087, 1113, 1145, 1246
BstMAI GTCTC 4 cut(s) 25, 238, 350, 628
BstMBI GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
BstMCI CGRYCG 2 cut(s) 50, 1229
BstMWI GCNNNNNNNGC 9 cut(s) 152, 597, 606, 615, 674, 685, 1104, 1278, 1306
BstNI CCWGG 1 cut(s) 1278
BstSCI CCNGG 2 cut(s) 316, 1276
BstSFI CTRYAG 5 cut(s) 310, 645, 774, 888, 912
BstUI CGCG 2 cut(s) 679, 1305
BstV1I GCAGC 4 cut(s) 5, 482, 659, 760
BstX2I RGATCY 1 cut(s) 1142
BstYI RGATCY 1 cut(s) 1142
BstZI CGGCCG 1 cut(s) 1226
BsuRI GGCC 6 cut(s) 316, 321, 801, 1209, 1228, 1256
BtgZI GCGATG 1 cut(s) 1254
BtsCI GGATG 7 cut(s) 33, 273, 481, 519, 627, 796, 1072
BtsIMutI CAGTG 2 cut(s) 382, 1168
Cac8I GCNNGC 5 cut(s) 666, 1041, 1080, 1207, 1307
CaiI CAGNNNCTG 1 cut(s) 930
CfoI GCGC 2 cut(s) 370, 679
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 1 cut(s) 315
CspCI CAANNNNNGTGG 3 cut(s) 561, 596, 1317
CviAII CATG 6 cut(s) 592, 727, 937, 1104, 1222, 1297
DdeI CTNAG 5 cut(s) 629, 651, 852, 857, 869
DpnI GATC 9 cut(s) 49, 381, 682, 795, 935, 1086, 1112, 1144, 1245
DpnII GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
DraI TTTAAA 1 cut(s) 396
EaeI YGGCCR 2 cut(s) 319, 1226
EagI CGGCCG 1 cut(s) 1226
EclXI CGGCCG 1 cut(s) 1226
Eco147I AGGCCT 1 cut(s) 801
Eco52I CGGCCG 1 cut(s) 1226
EcoRII CCWGG 1 cut(s) 1276
Esp3I CGTCTC 2 cut(s) 25, 350
FaeI CATG 6 cut(s) 595, 730, 940, 1107, 1225, 1300
FalI AAGNNNNNCTT 2 cut(s) 978, 1010
FaqI GGGAC 2 cut(s) 106, 833
FatI CATG 6 cut(s) 591, 726, 936, 1103, 1221, 1296
FauI CCCGC 1 cut(s) 208
FbaI TGATCA 3 cut(s) 379, 793, 1243
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 6 cut(s) 19, 471, 648, 774, 1226, 1254
FokI GGATG 7 cut(s) 40, 280, 488, 526, 634, 803, 1079
Fsp4HI GCNGC 6 cut(s) 19, 471, 648, 774, 1226, 1254
FspBI CTAG 1 cut(s) 483
GlaI GCGC 2 cut(s) 369, 678
GluI GCNGC 6 cut(s) 19, 471, 648, 774, 1226, 1254
GsuI CTGGAG 2 cut(s) 249, 1221
HaeIII GGCC 6 cut(s) 316, 321, 801, 1209, 1228, 1256
HapII CCGG 3 cut(s) 318, 615, 949
HhaI GCGC 2 cut(s) 370, 679
Hin1II CATG 6 cut(s) 595, 730, 940, 1107, 1225, 1300
Hin6I GCGC 2 cut(s) 368, 677
HinP1I GCGC 2 cut(s) 368, 677
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 36, 75, 231, 875, 979
HpaII CCGG 3 cut(s) 318, 615, 949
HphI GGTGA 3 cut(s) 81, 221, 529
Hpy166II GTNNAC 2 cut(s) 187, 222
Hpy188I TCNGA 9 cut(s) 47, 544, 642, 872, 926, 978, 1154, 1187, 1248
Hpy188III TCNNGA 4 cut(s) 72, 79, 235, 1003
Hpy8I GTNNAC 2 cut(s) 187, 222
Hpy99I CGWCG 2 cut(s) 188, 191
HpyAV CCTTC 5 cut(s) 225, 244, 383, 549, 812
HpyCH4III ACNGT 4 cut(s) 377, 529, 562, 829
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 6 cut(s) 647, 776, 908, 1043, 1070, 1107
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 597, 606, 615, 674, 685, 1104, 1278, 1306
HpyF3I CTNAG 5 cut(s) 629, 651, 852, 857, 869
HpySE526I ACGT 1 cut(s) 343
Hsp92II CATG 6 cut(s) 595, 730, 940, 1107, 1225, 1300
HspAI GCGC 2 cut(s) 368, 677
Ksp22I TGATCA 3 cut(s) 379, 793, 1243
Kzo9I GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
LmnI GCTCC 4 cut(s) 105, 1202, 1297, 1305
Lsp1109I GCAGC 4 cut(s) 5, 482, 659, 760
LweI GCATC 4 cut(s) 258, 321, 379, 1057
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 863, 1163
MalI GATC 9 cut(s) 49, 381, 682, 795, 935, 1086, 1112, 1144, 1245
MbiI CCGCTC 1 cut(s) 100
MboI GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
MboII GAAGA 4 cut(s) 424, 499, 518, 965
MflI RGATCY 1 cut(s) 1142
MluCI AATT 6 cut(s) 355, 459, 595, 880, 942, 1189
MlyI GAGTC 3 cut(s) 240, 884, 973
MmeI TCCRAC 1 cut(s) 366
MroXI GAANNNNTTC 1 cut(s) 744
MseI TTAA 6 cut(s) 261, 395, 768, 945, 984, 1128
MslI CAYNNNNRTG 3 cut(s) 375, 731, 785
MspA1I CMGCKG 4 cut(s) 227, 473, 650, 856
MspI CCGG 3 cut(s) 318, 615, 949
MspR9I CCNGG 2 cut(s) 318, 1278
MssI GTTTAAAC 1 cut(s) 396
Mva1269I GAATGC 3 cut(s) 433, 910, 1098
MvaI CCWGG 1 cut(s) 1278
MvnI CGCG 2 cut(s) 679, 1305
MwoI GCNNNNNNNGC 9 cut(s) 152, 597, 606, 615, 674, 685, 1104, 1278, 1306
NciI CCSGG 1 cut(s) 318
NdeII GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
NlaIII CATG 6 cut(s) 595, 730, 940, 1107, 1225, 1300
NlaIV GGNNCC 1 cut(s) 1144
NmeAIII GCCGAG 2 cut(s) 457, 1254
NmuCI GTSAC 1 cut(s) 1163
PceI AGGCCT 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 3 cut(s) 433, 910, 1098
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 2 cut(s) 36, 75
PkrI GCNGC 6 cut(s) 20, 472, 649, 775, 1227, 1255
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 3 cut(s) 239, 883, 973
PmeI GTTTAAAC 1 cut(s) 396
PpsI GAGTC 3 cut(s) 239, 883, 973
Psp6I CCWGG 1 cut(s) 1276
PspGI CCWGG 1 cut(s) 1276
PspN4I GGNNCC 1 cut(s) 1144
PspPI GGNCC 1 cut(s) 315
PstI CTGCAG 2 cut(s) 649, 778
PstNI CAGNNNCTG 1 cut(s) 930
PsuI RGATCY 1 cut(s) 1142
PvuI CGATCG 1 cut(s) 50
PvuII CAGCTG 3 cut(s) 227, 650, 856
RseI CAYNNNNRTG 3 cut(s) 375, 731, 785
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 6 cut(s) 261, 395, 768, 945, 984, 1128
SatI GCNGC 6 cut(s) 19, 471, 648, 774, 1226, 1254
Sau3AI GATC 9 cut(s) 47, 379, 680, 793, 933, 1084, 1110, 1142, 1243
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 3 cut(s) 240, 884, 973
ScrFI CCNGG 2 cut(s) 318, 1278
SfaNI GCATC 4 cut(s) 258, 321, 379, 1057
SfcI CTRYAG 5 cut(s) 310, 645, 774, 888, 912
SgrDI CGTCGACG 1 cut(s) 185
SmiMI CAYNNNNRTG 3 cut(s) 375, 731, 785
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 6 cut(s) 355, 459, 595, 880, 942, 1189
SseBI AGGCCT 1 cut(s) 801
SspMI CTAG 1 cut(s) 483
StuI AGGCCT 1 cut(s) 801
StyD4I CCNGG 2 cut(s) 316, 1276
TaaI ACNGT 4 cut(s) 377, 529, 562, 829
TaiI ACGT 1 cut(s) 346
TaqI TCGA 5 cut(s) 50, 73, 186, 236, 248
TasI AATT 6 cut(s) 355, 459, 595, 880, 942, 1189
TauI GCSGC 2 cut(s) 1228, 1256
TfiI GAWTC 2 cut(s) 36, 75
Tru1I TTAA 6 cut(s) 261, 395, 768, 945, 984, 1128
Tru9I TTAA 6 cut(s) 261, 395, 768, 945, 984, 1128
TscAI CASTG 2 cut(s) 382, 1168
TseFI GTSAC 1 cut(s) 1163
TseI GCWGC 4 cut(s) 18, 470, 647, 773
Tsp45I GTSAC 1 cut(s) 1163
TspDTI ATGAA 4 cut(s) 608, 715, 794, 1210
TspGWI ACGGA 2 cut(s) 23, 172
TspRI CASTG 2 cut(s) 382, 1168
XmiI GTMKAC 1 cut(s) 186
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.