Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010128

Physical Location & Seq

Genomic Coordinates Reverse (-)

23233434 .. 23236447

Gene Structure 3014 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G62750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGCGGCGGATGCTCTGAGAATCTCGAGTTCTAGTTCTGGTTCGTTGGTTTGTAATCTTAATGGTTCACAAAGACGACCTGTTCTTCTTCCTCTGTCTCATCGTGCTACCTTTCTGGGTCTTCCTCCTCGTGCTTCATCTTCTTCGATTTCTTCTTCAATTCCTCAGTTTCTGGGTACTTCTCGTATTGGGCTAGGTTCTTCCAAGCTCTCACAAAAGAAGAAACAATTCTCAGTCTTCGCTGCAGCTGAAGCCGAGGCGAAGCGGGCTGTACCGCTTAAAGATTACCGAAACATCGGTATTATGGCTCACATAGACGCGGGAAAGACGACAACAACCGAGAGGATTCTTTACTACACAGGAAGAAACTACAAAATCGGTGAAGTGCATGAAGGGACAGCTACAATGGATTGGATGGAACAAGAGCAAGAACGAGGAATCACCATTACTTCAGCTGCAACCACAACGTTTTGGGATAAACACAGGATCAATATTATTGATACACCGGGTCACGTCGATTTCACTCTGGAAGTTGAACGTGCTTTAAGAGTTCTCGATGGAGCTATATGCTTGTTCGATAGTGTTGCTGGTGTTGAGCCTCAGTCTGAGACTGTGTGGAGACAAGCTGATAAATATGGTGTGCCTAGGATTTGCTTTGTTAACAAGATGGACAGGCTTGGAGCTAACTTTTTCAGGACTAGGGACATGATTGTGACCAATTTGGGTGCTAAGCCGCTGGTGCTTCAGATACCAATTGGTGCTGAAGATGTCTTTAAAGGTGTTGTTGATCTTGTGAGGATGAAAGCTATAGTTTGGTCAGGAGAAGAGCTTGGTGCAAAGTTCAGTTACGAGGATATTCCAGAAGATCTTGAAGATTTGGCTCAAGAGTATCGGGCGGCGATGATGGAATTGATTGTTGATTTGGATGATGAGGTCATGGAGAACTATTTAGAAGGAGTTGAGCCTGACGAGGCCACGGTAAAGAGATTGGTTAGAAAAGGAACCATCACGGGCAAGTTTGTGCCAATTCTATGTGGTTCAGCCTTTAAGAACAAAGGAGTGCAGCCATTGCTCGACGCTGTGGTCGATTATCTGCCTTCGCCCGTTGAAGTCCCGCCGATGAATGGAACAGATCCTGAGAACCCGGAAATTACCATTATACGGAAACCAGATGATGACGAGCCATTTGCTGGGCTAGCATTCAAGATCATGAGTGATCCTTTTGTGGGTTCTCTTACATTTGTGAGGGTTTACTCAGGGAAAATCTCAGCTGGTTCTTACGTGTTGAATGCTAACAAAGGAAAGAAGGAGAGAATCGGAAGACTCTTGGAAATGCACGCAAACAGCAGAGAAGATGTTAAAGTGGCTTTGACAGGTGACATTATCGCTCTTGCGGGTCTCAAAGATACAATCACTGGTGAAACCTTGAGTGATCCAGAAAACCCCGTTGTTCTTGAACGAATGGACTTCCCTGATCCCGTCATCAAGGTAGCGATTGAGCCAAAAACAAAAGCAGACATCGACAAAATGGCGACAGGGTTGATCAAGCTCGCTCAAGAAGACCCGTCTTTCCATTTTTCTCGTGATGAAGAGATGAACCAAACCGTCATTGAAGGAATGGGAGAACTTCATCTCGAGATTATCGTTGACAGACTTAAAAGAGAGTTCAAGGTGGAGGCTAACGTTGGTGCACCGCAAGTCAACTACCGTGAGAGTATCTCCAAAATAGCTGAAGTGAAATACACACACAAGAAGCAATCAGGTGGACAAGGTCAGTTTGCTGATATTACAGTCCGGTTTGAGCCATTGGAAGCAGGGTCAGGATACGAATTCAAGAGTGAGATCAAAGGAGGAGCTGTGCCAAGAGAGTATATTCCTGGTGTCATGAAAGGACTTGAGGAGTGTATGAGCACCGGTGTGCTTGCTGGTTTCCCGGTTGTTGATGTCCGTGCTTGTCTAGTTGACGGATCTTACCATGACGTAGACTCAAGCGTGCTAGCTTTCCAGCTTGCTGCAAGAGGAGCTTTCCGTGAAGGGATGAGGAAAGCGGGTCCGAGAATGCTTGAACCTATCATGAGAGTTGAAGTTGTTACACCAGAAGAACATCTTGGTGATGTCATTGGTGATCTTAACTCACGAAGAGGTCAAATTAACAGCTTCGGAGACAAACCCGGTGGTCTCAAGGTGGTCGACTCTCTGGTTCCATTGGCAGAGATGTTTCAGTACGTGAGTACATTGAGAGGGATGACAAAAGGTCGAGCTTCTTACACAATGCAACTGGCTAAGTTCGACGTTGTTCCTCAACATATTCAGAACCAACTTTCATCCAAGGATCAAGAAGTTGCTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009845 GO:0009941 GO:0009987 GO:0016043 GO:0017076 GO:0030554 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0071840 GO:0090351 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

783

Amino Acids

86.06

Weight (kDa)

5.43

Isoelectric Point (pI)

34.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	94 - 367	1.8e-67	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	395 - 477	5.5e-16	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	410 - 476	2.4e-16	Elongation factor Tu domain 2
EFG_III	PF14492	490 - 563	1.1e-31	Elongation Factor G, domain III
RF3_C	PF16658	500 - 578	1.4e-06	Class II release factor RF3, C-terminal domain
EFG_IV	PF03764	565 - 683	2.1e-46	Elongation factor G, domain IV
EFG_C	PF00679	687 - 773	1.2e-25	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62750
fragaria_vesca	FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 FvH4_5g00390
malus_domestica	MD04G1081800.v1.1 MD06G1065300.v1.1
prunus_persica	Prupe.5G076600_v2.0.a1 Prupe.5G076600_v2.0.a1
pyrus_communis	pycom04g07370 pycom06g05630
rosa_chinensis	RchiOBHm_Chr7g0202121
rosa_laevigata	RLG00000003624
rosa_multiflora	Rmu_ssc0000228.1_g000002
rosa_roxburghii	Rroxscaffold_3G00254840
rosa_rugosa	Rorug07G0068100
rosa_samantha	Rh7AG195600 Rh7BG196900 Rh7CG206700 Rh7DG202200
rosa_wichuraiana	Rw7G017130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1082
AccB7I	CCANNNNNTGG	1 cut(s)	1190
AccI	GTMKAC	2 cut(s)	1983, 2190
AccII	CGCG	1 cut(s)	320
AclI	AACGTT	2 cut(s)	467, 1683
AclWI	GGATC	7 cut(s)	494, 1127, 1211, 1427, 1469, 1975, 2340
AcsI	RAATTY	1 cut(s)	1829
AcuI	CTGAAG	5 cut(s)	270, 435, 728, 783, 1752
AfaI	GTAC	4 cut(s)	178, 273, 2225, 2233
AfiI	CCNNNNNNNGG	4 cut(s)	1124, 1161, 1190, 1226
AflIII	ACRYGT	1 cut(s)	1281
AgeI	ACCGGT	1 cut(s)	1913
AhdI	GACNNNNNGTC	1 cut(s)	2253
AjiI	CACGTC	1 cut(s)	514
AjnI	CCWGG	1 cut(s)	1876
AjuI	GAANNNNNNNTTGG	2 cut(s)	2091, 2123
AleI	CACNNNNGTG	1 cut(s)	1916
Alw21I	GWGCWC	2 cut(s)	1693, 1913
Alw26I	GTCTC	6 cut(s)	102, 602, 613, 1403, 2157, 2183
Alw44I	GTGCAC	1 cut(s)	1689
AlwI	GGATC	7 cut(s)	494, 1127, 1211, 1427, 1469, 1975, 2340
AlwNI	CAGNNNCTG	2 cut(s)	172, 1136
Ama87I	CYCGRG	2 cut(s)	25, 1634
AoxI	GGCC	1 cut(s)	972
ApaLI	GTGCAC	1 cut(s)	1689
ApeKI	GCWGC	6 cut(s)	242, 245, 455, 1063, 2012, 2345
ApoI	RAATTY	1 cut(s)	1829
ArsI	GACNNNNNNTTYG	2 cut(s)	1169, 1201
AsiGI	ACCGGT	1 cut(s)	1913
Asp700I	GAANNNNTTC	1 cut(s)	227
AspA2I	CCTAGG	1 cut(s)	644
AspS9I	GGNCC	1 cut(s)	2051
AsuC2I	CCSGG	4 cut(s)	507, 1145, 1934, 2172
AsuHPI	GGTGA	6 cut(s)	392, 433, 1388, 1430, 2123, 2135
AsuNHI	GCTAGC	2 cut(s)	1195, 1996
AvaI	CYCGRG	2 cut(s)	25, 1634
AvaII	GGWCC	1 cut(s)	2051
AvrII	CCTAGG	1 cut(s)	644
BaeGI	GKGCMC	1 cut(s)	1693
BauI	CACGAG	2 cut(s)	129, 1581
BbsI	GAAGAC	4 cut(s)	113, 229, 1327, 1566
Bbv12I	GWGCWC	2 cut(s)	1693, 1913
BbvI	GCAGC	6 cut(s)	229, 257, 442, 1075, 1999, 2332
BccI	CCATC	5 cut(s)	409, 551, 661, 898, 1013
BcgI	CGANNNNNNTGC	4 cut(s)	566, 600, 1169, 1203
BciT130I	CCWGG	1 cut(s)	1878
BciVI	GTATCC	1 cut(s)	1817
BclI	TGATCA	1 cut(s)	1542
BcnI	CCSGG	4 cut(s)	507, 1145, 1934, 2172
BcoDI	GTCTC	6 cut(s)	102, 602, 613, 1403, 2157, 2183
BfaI	CTAG	7 cut(s)	33, 194, 645, 699, 1196, 1958, 1997
BfmI	CTRYAG	2 cut(s)	243, 807
BfuI	GTATCC	1 cut(s)	1817
BglII	AGATCT	1 cut(s)	865
BisI	GCNGC	9 cut(s)	6, 243, 246, 456, 734, 897, 1064, 2013, 2346
BlnI	CCTAGG	1 cut(s)	644
BlpI	GCTNAGC	1 cut(s)	729
BlsI	GCNGC	9 cut(s)	7, 244, 247, 457, 735, 898, 1065, 2014, 2347
Bme1390I	CCNGG	5 cut(s)	507, 1145, 1878, 1934, 2172
Bme18I	GGWCC	1 cut(s)	2051
BmeRI	GACNNNNNGTC	1 cut(s)	2253
BmeT110I	CYCGRG	2 cut(s)	25, 1634
BmgBI	CACGTC	1 cut(s)	514
BmgT120I	GGNCC	1 cut(s)	2051
BmiI	GGNNCC	3 cut(s)	1003, 2052, 2202
BmrFI	CCNGG	5 cut(s)	507, 1145, 1878, 1934, 2172
BmtI	GCTAGC	2 cut(s)	1199, 2000
BpiI	GAAGAC	4 cut(s)	113, 229, 1327, 1566
BplI	GAGNNNNNCTC	2 cut(s)	1703, 1735
Bpu1102I	GCTNAGC	1 cut(s)	729
BpuEI	CTTGAG	6 cut(s)	867, 1447, 1539, 1916, 1972, 2165
BpuMI	CCSGG	4 cut(s)	507, 1145, 1934, 2172
BsaAI	YACGTR	2 cut(s)	1282, 2227
BsaI	GGTCTC	2 cut(s)	1403, 2183
BsaJI	CCNNGG	4 cut(s)	255, 644, 975, 2328
BsaWI	WCCGGW	2 cut(s)	1794, 1913
BsaXI	ACNNNNNCTCC	2 cut(s)	1842, 1872
Bsc4I	CCNNNNNNNGG	4 cut(s)	1124, 1161, 1190, 1226
Bse118I	RCCGGY	1 cut(s)	1913
Bse1I	ACTGG	2 cut(s)	1420, 2283
Bse3DI	GCAATG	1 cut(s)	1067
BseBI	CCWGG	1 cut(s)	1878
BseDI	CCNNGG	4 cut(s)	255, 644, 975, 2328
BseGI	GGATG	7 cut(s)	16, 420, 804, 931, 2043, 2250, 2324
BseLI	CCNNNNNNNGG	4 cut(s)	1124, 1161, 1190, 1226
BseMI	GCAATG	1 cut(s)	1067
BseMII	CTCAG	8 cut(s)	8, 179, 246, 597, 614, 1128, 1269, 1281
BseNI	ACTGG	2 cut(s)	1420, 2283
BseRI	GAGGAG	4 cut(s)	117, 1866, 1913, 2034
BseSI	GKGCMC	1 cut(s)	1693
BseXI	GCAGC	6 cut(s)	229, 257, 442, 1075, 1999, 2332
BseYI	CCCAGC	1 cut(s)	1190
BsgI	GTGCAG	1 cut(s)	1082
Bsh1236I	CGCG	1 cut(s)	320
BshFI	GGCC	1 cut(s)	974
BshTI	ACCGGT	1 cut(s)	1913
BsiHKAI	GWGCWC	2 cut(s)	1693, 1913
BsiHKCI	CYCGRG	2 cut(s)	25, 1634
BsiSI	CCGG	6 cut(s)	506, 1145, 1795, 1914, 1934, 2172
BslFI	GGGAC	3 cut(s)	409, 716, 1097
BslI	CCNNNNNNNGG	4 cut(s)	1124, 1161, 1190, 1226
BsmAI	GTCTC	6 cut(s)	102, 602, 613, 1403, 2157, 2183
BsmFI	GGGAC	3 cut(s)	409, 716, 1097
BsmI	GAATGC	3 cut(s)	1199, 1294, 2064
BsnI	GGCC	1 cut(s)	974
Bso31I	GGTCTC	2 cut(s)	1403, 2183
BsoBI	CYCGRG	2 cut(s)	25, 1634
Bsp1286I	GDGCHC	2 cut(s)	1693, 1913
Bsp1720I	GCTNAGC	1 cut(s)	729
BspANI	GGCC	1 cut(s)	974
BspCNI	CTCAG	8 cut(s)	9, 178, 245, 598, 613, 1129, 1268, 1280
BspFNI	CGCG	1 cut(s)	320
BspHI	TCATGA	3 cut(s)	1209, 1884, 2073
BspLI	GGNNCC	3 cut(s)	1003, 2052, 2202
BspMAI	CTGCAG	1 cut(s)	247
BspOI	GCTAGC	2 cut(s)	1199, 2000
BspPI	GGATC	7 cut(s)	494, 1127, 1211, 1427, 1469, 1975, 2340
BspQI	GCTCTTC	1 cut(s)	819
BspTNI	GGTCTC	2 cut(s)	1403, 2183
BsrDI	GCAATG	1 cut(s)	1067
BsrFI	RCCGGY	1 cut(s)	1913
BsrI	ACTGG	2 cut(s)	1420, 2283
BssAI	RCCGGY	1 cut(s)	1913
BssECI	CCNNGG	4 cut(s)	255, 644, 975, 2328
BssSI	CACGAG	2 cut(s)	129, 1581
BssT1I	CCWWGG	2 cut(s)	644, 2328
Bst2BI	CACGAG	2 cut(s)	129, 1581
Bst2UI	CCWGG	1 cut(s)	1878
Bst4CI	ACNGT	5 cut(s)	613, 979, 1606, 1709, 1792
Bst6I	CTCTTC	3 cut(s)	819, 1584, 2134
BstAPI	GCANNNNNTGC	1 cut(s)	1069
BstBAI	YACGTR	2 cut(s)	1282, 2227
BstC8I	GCNNGC	8 cut(s)	267, 1197, 1338, 1551, 1923, 1994, 1998, 2010
BstDSI	CCRYGG	1 cut(s)	975
BstF5I	GGATG	7 cut(s)	16, 420, 804, 931, 2043, 2250, 2324
BstFNI	CGCG	1 cut(s)	320
BstMAI	GTCTC	6 cut(s)	102, 602, 613, 1403, 2157, 2183
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 251, 266, 739, 1069, 1196, 2021
BstNI	CCWGG	1 cut(s)	1878
BstSCI	CCNGG	5 cut(s)	505, 1143, 1876, 1932, 2170
BstSFI	CTRYAG	2 cut(s)	243, 807
BstSLI	GKGCMC	1 cut(s)	1693
BstUI	CGCG	1 cut(s)	320
BstV1I	GCAGC	6 cut(s)	229, 257, 442, 1075, 1999, 2332
BstV2I	GAAGAC	4 cut(s)	113, 229, 1327, 1566
BstX2I	RGATCY	3 cut(s)	865, 1132, 1967
BstYI	RGATCY	3 cut(s)	865, 1132, 1967
BsuI	GTATCC	1 cut(s)	1817
BsuRI	GGCC	1 cut(s)	974
BtgI	CCRYGG	1 cut(s)	975
BtgZI	GCGATG	1 cut(s)	914
BtrI	CACGTC	1 cut(s)	514
BtsCI	GGATG	7 cut(s)	16, 420, 804, 931, 2043, 2250, 2324
BtsIMutI	CAGTG	1 cut(s)	1413
Cac8I	GCNNGC	8 cut(s)	267, 1197, 1338, 1551, 1923, 1994, 1998, 2010
CaiI	CAGNNNCTG	2 cut(s)	172, 1136
CciI	TCATGA	3 cut(s)	1209, 1884, 2073
Cfr10I	RCCGGY	1 cut(s)	1913
Cfr13I	GGNCC	1 cut(s)	2051
CseI	GACGC	2 cut(s)	326, 1085
Csp6I	GTAC	4 cut(s)	177, 272, 2224, 2232
CspAI	ACCGGT	1 cut(s)	1913
CspCI	CAANNNNNGTGG	2 cut(s)	2155, 2190
CviAII	CATG	7 cut(s)	389, 706, 937, 1210, 1885, 1976, 2074
CviQI	GTAC	4 cut(s)	177, 272, 2224, 2232
DraI	TTTAAA	1 cut(s)	775
DrdI	GACNNNNNNGTC	1 cut(s)	1082
DriI	GACNNNNNGTC	1 cut(s)	2253
DseDI	GACNNNNNNGTC	1 cut(s)	1082
Eam1104I	CTCTTC	3 cut(s)	819, 1584, 2134
Eam1105I	GACNNNNNGTC	1 cut(s)	2253
EarI	CTCTTC	3 cut(s)	819, 1584, 2134
EciI	GGCGGA	1 cut(s)	23
Eco130I	CCWWGG	2 cut(s)	644, 2328
Eco31I	GGTCTC	2 cut(s)	1403, 2183
Eco47I	GGWCC	1 cut(s)	2051
Eco57I	CTGAAG	5 cut(s)	270, 435, 728, 783, 1752
Eco88I	CYCGRG	2 cut(s)	25, 1634
EcoRI	GAATTC	1 cut(s)	1829
EcoRII	CCWGG	1 cut(s)	1876
EcoT14I	CCWWGG	2 cut(s)	644, 2328
ErhI	CCWWGG	2 cut(s)	644, 2328
FaeI	CATG	7 cut(s)	392, 709, 940, 1213, 1888, 1979, 2077
FalI	AAGNNNNNCTT	4 cut(s)	2008, 2040, 2091, 2123
FaqI	GGGAC	3 cut(s)	409, 716, 1097
FatI	CATG	7 cut(s)	388, 705, 936, 1209, 1884, 1975, 2073
FauI	CCCGC	5 cut(s)	258, 313, 1122, 1387, 2041
FbaI	TGATCA	1 cut(s)	1542
FblI	GTMKAC	2 cut(s)	1983, 2190
Fnu4HI	GCNGC	9 cut(s)	6, 243, 246, 456, 734, 897, 1064, 2013, 2346
FokI	GGATG	7 cut(s)	23, 427, 811, 938, 2050, 2257, 2311
Fsp4HI	GCNGC	9 cut(s)	6, 243, 246, 456, 734, 897, 1064, 2013, 2346
FspBI	CTAG	7 cut(s)	33, 194, 645, 699, 1196, 1958, 1997
GluI	GCNGC	9 cut(s)	6, 243, 246, 456, 734, 897, 1064, 2013, 2346
GsaI	CCCAGC	1 cut(s)	1194
HaeIII	GGCC	1 cut(s)	974
HapII	CCGG	6 cut(s)	506, 1145, 1795, 1914, 1934, 2172
HgaI	GACGC	2 cut(s)	326, 1085
Hin1II	CATG	7 cut(s)	392, 709, 940, 1213, 1888, 1979, 2077
HincII	GTYRAC	5 cut(s)	661, 1648, 1702, 1963, 2191
HindII	GTYRAC	5 cut(s)	661, 1648, 1702, 1963, 2191
HinfI	GANTC	7 cut(s)	21, 346, 438, 1314, 1323, 1985, 2192
HpaI	GTTAAC	1 cut(s)	661
HpaII	CCGG	6 cut(s)	506, 1145, 1795, 1914, 1934, 2172
HphI	GGTGA	6 cut(s)	392, 433, 1388, 1430, 2123, 2135
Hpy188I	TCNGA	7 cut(s)	18, 607, 747, 1319, 2055, 2162, 2313
Hpy99I	CGWCG	3 cut(s)	518, 1079, 2294
HpyAV	CCTTC	6 cut(s)	386, 947, 1107, 1300, 1607, 2027
HpyCH4III	ACNGT	5 cut(s)	613, 979, 1606, 1709, 1792
HpyCH4IV	ACGT	8 cut(s)	467, 513, 538, 1281, 1683, 1980, 2226, 2292
HpyCH4V	TGCA	9 cut(s)	245, 388, 458, 836, 1063, 1336, 1691, 2015, 2275
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 251, 266, 739, 1069, 1196, 2021
HpySE526I	ACGT	8 cut(s)	467, 513, 538, 1281, 1683, 1980, 2226, 2292
Hsp92II	CATG	7 cut(s)	392, 709, 940, 1213, 1888, 1979, 2077
Ksp22I	TGATCA	1 cut(s)	1542
KspAI	GTTAAC	1 cut(s)	661
LguI	GCTCTTC	1 cut(s)	819
LmnI	GCTCC	4 cut(s)	560, 680, 1853, 2021
Lsp1109I	GCAGC	6 cut(s)	229, 257, 442, 1075, 1999, 2332
MaeI	CTAG	7 cut(s)	33, 194, 645, 699, 1196, 1958, 1997
MaeII	ACGT	8 cut(s)	467, 513, 538, 1281, 1683, 1980, 2226, 2292
MaeIII	GTNAC	5 cut(s)	509, 712, 845, 1376, 2089
MfeI	CAATTG	1 cut(s)	753
MflI	RGATCY	3 cut(s)	865, 1132, 1967
MhlI	GDGCHC	2 cut(s)	1693, 1913
MluCI	AATT	9 cut(s)	159, 227, 718, 753, 908, 1026, 1149, 1829, 2148
MlyI	GAGTC	3 cut(s)	1317, 1979, 2186
MroXI	GAANNNNTTC	1 cut(s)	227
MslI	CAYNNNNRTG	3 cut(s)	710, 1916, 2109
MspA1I	CMGCKG	4 cut(s)	248, 455, 736, 1271
MspI	CCGG	6 cut(s)	506, 1145, 1795, 1914, 1934, 2172
MspR9I	CCNGG	5 cut(s)	507, 1145, 1878, 1934, 2172
MunI	CAATTG	1 cut(s)	753
Mva1269I	GAATGC	3 cut(s)	1199, 1294, 2064
MvaI	CCWGG	1 cut(s)	1878
MvnI	CGCG	1 cut(s)	320
MwoI	GCNNNNNNNGC	7 cut(s)	11, 251, 266, 739, 1069, 1196, 2021
NciI	CCSGG	4 cut(s)	507, 1145, 1934, 2172
NheI	GCTAGC	2 cut(s)	1195, 1996
NlaIII	CATG	7 cut(s)	392, 709, 940, 1213, 1888, 1979, 2077
NlaIV	GGNNCC	3 cut(s)	1003, 2052, 2202
NmeAIII	GCCGAG	1 cut(s)	280
NmuCI	GTSAC	3 cut(s)	509, 712, 1376
OliI	CACNNNNGTG	1 cut(s)	1916
PaeR7I	CTCGAG	2 cut(s)	25, 1634
PagI	TCATGA	3 cut(s)	1209, 1884, 2073
PciSI	GCTCTTC	1 cut(s)	819
PcsI	WCGNNNNNNNCGW	2 cut(s)	1083, 1641
PctI	GAATGC	3 cut(s)	1199, 1294, 2064
PdmI	GAANNNNTTC	1 cut(s)	227
PfeI	GAWTC	4 cut(s)	21, 346, 438, 1314
PflFI	GACNNNGTC	1 cut(s)	1770
PflMI	CCANNNNNTGG	1 cut(s)	1190
PinAI	ACCGGT	1 cut(s)	1913
PkrI	GCNGC	9 cut(s)	7, 244, 247, 457, 735, 898, 1065, 2014, 2347
PleI	GAGTC	3 cut(s)	1317, 1979, 2186
PpsI	GAGTC	3 cut(s)	1317, 1979, 2186
Ppu21I	YACGTR	2 cut(s)	1282, 2227
Psp1406I	AACGTT	2 cut(s)	467, 1683
Psp6I	CCWGG	1 cut(s)	1876
PspFI	CCCAGC	1 cut(s)	1190
PspGI	CCWGG	1 cut(s)	1876
PspN4I	GGNNCC	3 cut(s)	1003, 2052, 2202
PspPI	GGNCC	1 cut(s)	2051
PstI	CTGCAG	1 cut(s)	247
PstNI	CAGNNNCTG	2 cut(s)	172, 1136
PsuI	RGATCY	3 cut(s)	865, 1132, 1967
PsyI	GACNNNGTC	1 cut(s)	1770
PvuII	CAGCTG	3 cut(s)	248, 455, 1271
RsaI	GTAC	4 cut(s)	178, 273, 2225, 2233
RsaNI	GTAC	4 cut(s)	177, 272, 2224, 2232
RseI	CAYNNNNRTG	3 cut(s)	710, 1916, 2109
SalI	GTCGAC	1 cut(s)	2189
SapI	GCTCTTC	1 cut(s)	819
SatI	GCNGC	9 cut(s)	6, 243, 246, 456, 734, 897, 1064, 2013, 2346
Sau96I	GGNCC	1 cut(s)	2051
SchI	GAGTC	3 cut(s)	1317, 1979, 2186
ScrFI	CCNGG	5 cut(s)	507, 1145, 1878, 1934, 2172
SduI	GDGCHC	2 cut(s)	1693, 1913
SfcI	CTRYAG	2 cut(s)	243, 807
Sfr274I	CTCGAG	2 cut(s)	25, 1634
SgrAI	CRCCGGYG	1 cut(s)	1913
SinI	GGWCC	1 cut(s)	2051
SlaI	CTCGAG	2 cut(s)	25, 1634
SmiMI	CAYNNNNRTG	3 cut(s)	710, 1916, 2109
SmlI	CTYRAG	8 cut(s)	25, 882, 1426, 1554, 1634, 1895, 1987, 2180
SmoI	CTYRAG	8 cut(s)	25, 882, 1426, 1554, 1634, 1895, 1987, 2180
Sse9I	AATT	9 cut(s)	159, 227, 718, 753, 908, 1026, 1149, 1829, 2148
SspI	AATATT	1 cut(s)	493
SspMI	CTAG	7 cut(s)	33, 194, 645, 699, 1196, 1958, 1997
StyD4I	CCNGG	5 cut(s)	505, 1143, 1876, 1932, 2170
StyI	CCWWGG	2 cut(s)	644, 2328
TaaI	ACNGT	5 cut(s)	613, 979, 1606, 1709, 1792
TaiI	ACGT	8 cut(s)	470, 516, 541, 1284, 1686, 1983, 2229, 2295
TasI	AATT	9 cut(s)	159, 227, 718, 753, 908, 1026, 1149, 1829, 2148
TatI	WGTACW	1 cut(s)	2231
TauI	GCSGC	3 cut(s)	8, 736, 899
TfiI	GAWTC	4 cut(s)	21, 346, 438, 1314
TscAI	CASTG	1 cut(s)	1420
TseFI	GTSAC	3 cut(s)	509, 712, 1376
TseI	GCWGC	6 cut(s)	242, 245, 455, 1063, 2012, 2345
Tsp45I	GTSAC	3 cut(s)	509, 712, 1376
TspDTI	ATGAA	9 cut(s)	126, 405, 815, 1136, 1602, 1610, 1619, 1901, 2313
TspGWI	ACGGA	4 cut(s)	1177, 1937, 1980, 2018
TspRI	CASTG	1 cut(s)	1420
Tth111I	GACNNNGTC	1 cut(s)	1770
Van91I	CCANNNNNTGG	1 cut(s)	1190
VneI	GTGCAC	1 cut(s)	1689
VpaK11BI	GGWCC	1 cut(s)	2051
XapI	RAATTY	1 cut(s)	1829
XhoI	CTCGAG	2 cut(s)	25, 1634
XmaJI	CCTAGG	1 cut(s)	644
XmiI	GTMKAC	2 cut(s)	1983, 2190
XmnI	GAANNNNTTC	1 cut(s)	227
XspI	CTAG	7 cut(s)	33, 194, 645, 699, 1196, 1958, 1997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.