Rh7BG196900

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010128
Physical Location & Seq
Forward (+)
16154895 .. 16159069
4175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG196900.1

Sequence Viewer

Length: 1719 bp
ATGGCTACAGAGTCAGTCAGAGTAGTCCACAGTTTCAGCTTCAATGGGTCTCAGCCAAGGCCTACCATCCCTCTCTCCCCAGCTCGCTTTCTGGGTCTTCGTCCTCCTCGTTCTTCTTCTTCTTCTTCTTCTTCTCTCACCTCGTCTTCACTTTCCCAGTTCTTTGGGAATCTGCGTCTCACCTCCAACTCCTCAAAGACCTCTCATTTGCGCCAACACAGCCGGAGAAACCTCTCTGTTTTCGCCATGGCTGCCGATGATGGAAAGCGTGCAGTACCATTGGTAGATTATCGGAATATTGGAATTATGGCTCACATAGATGCAGGGAAGACTACTACAACTGAACGGATTTTGTACTATACTGGAAGAAACTATAAAATTGGGGAGGTGCATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCAGCAACTACAACATTTTGGGACAAACACAGAATTAACATTATTGATACTCCTGGCCATGTGGACTTCACCCTCGAAGTGGAGCGAGCTCTCAGGGTACTTGATGGCGCAATATGCTTGTTTGACAGTGTTGCTGGGGTGGAACCTCAGTCTGAAACTGTGTGGAGGCAAGCTGATAAATATGGGGTGCCCAGGATTTGCTTTGTCAACAAAATGGATCGTCTTGGGGCAAACTTTTTCCGCACAAGGGACATGATAGTGACAAATTTGGGTGCCAAACCACTTGTACTTCAAATCCCAGTTGGTGCCGAAGATAATTTTAAGGGAGTTATTGATCTTGTGAAGATGAGAGCTATACTTTGGTCAGGAGAAGAATTGGGTGCAAAGTTTTCGTATGAGGATATTCCATCTGATCTTCAGGAGTTGGCTGACGAATACAGGTCACTGATGATAGAAACCATAGTTGAGTTGGATGATGAAGCTATGGAGGGCTATCTAGAAGGAGTTGAACCTGATGAGGCCACCATTAAGAAATTGATTAGGCAAGGAACCATCTCAGCTAGTTTTGTGCCAGTATTATGTGGCTCAGCTTTTAAAAATAAGGGGGTTCAACCATTGCTTGATGCTGTCGTGGATTATTTACCTTCACCGCTTGATGTGCCACCAATGCAGGGAACTGATGCAGATAACCCAGAAATCACAATTGAGAGGGCTGCAAGTGATGATGAACCATTTGCTGGGCTGGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATCTATGCAGGAAAGCTAACTGCTGGATCATACGTGCTGAATGCAAACAAGGGGAAAAAGGAGAGGATCGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTTAAGGTTGCTTTAGCTGGTGATATTGTTGCTCTTGCAGGTCTAAAAGATACTGTTACTGGTGAAACATTGAGTGACCCTGAGCATCCTATTGTGCTTGAACGTATGGAATTCCCTGATCCTGTGATTAAGGTGGCAATTGAACCCAAAACTAAAGCCGATGTTGATAAGATGGGAGCTGGTTTGGTTAAACTTGCTCAGGAAGACCCTTCGTTCCACTTCTCAAGGGATGAAGAGGTCAATCAGACAGTGATCGAAGGAATGGGTGAATTGCATCTTGAGATTATTGTTGATCGCCTCAAGAGGGAATTCAAGGTATATATCTGCTACTCCATGTTGCTGATTGTCAATTTCATGTAG

Protein Analysis

572

Amino Acids

63.33

Weight (kDa)

5.22

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 97 - 368 1.1e-67 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 396 - 478 8.8e-17 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 411 - 477 1.2e-16 Elongation factor Tu domain 2
EFG_III PF14492 491 - 560 2.6e-30 Elongation Factor G, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1388
AccB1I GGYRCC 3 cut(s) 643, 728, 761
AccB7I CCANNNNNTGG 1 cut(s) 1193
AccI GTMKAC 1 cut(s) 1324
AciI CCGC 2 cut(s) 697, 1106
AclWI GGATC 7 cut(s) 681, 1214, 1227, 1240, 1285, 1325, 1472
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 3 cut(s) 721, 1469, 1667
AcuI CTGAAG 1 cut(s) 857
AfaI GTAC 4 cut(s) 276, 356, 555, 744
AfiI CCNNNNNNNGG 6 cut(s) 535, 703, 1127, 1193, 1229, 1663
AgsI TTSAA 8 cut(s) 43, 749, 965, 1067, 1207, 1460, 1502, 1672
AjnI CCWGG 2 cut(s) 508, 647
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 54, 182
AlwI GGATC 7 cut(s) 681, 1214, 1227, 1240, 1285, 1325, 1472
AoxI GGCC 3 cut(s) 59, 511, 975
ApeKI GCWGC 3 cut(s) 251, 458, 1169
ApoI RAATTY 3 cut(s) 721, 1469, 1667
ArsI GACNNNNNNTTYG 2 cut(s) 190, 222
AspLEI GCGC 2 cut(s) 213, 566
AsuHPI GGTGA 7 cut(s) 130, 172, 517, 1095, 1391, 1433, 1637
BaeGI GKGCMC 1 cut(s) 648
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 1232
BanI GGYRCC 3 cut(s) 643, 728, 761
BanII GRGCYC 1 cut(s) 547
BbsI GAAGAC 4 cut(s) 89, 138, 335, 1569
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 3 cut(s) 238, 470, 1156
BccI CCATC 7 cut(s) 74, 254, 412, 554, 871, 1016, 1525
BcgI CGANNNNNNTGC 2 cut(s) 828, 862
BciT130I CCWGG 2 cut(s) 510, 649
BcoDI GTCTC 2 cut(s) 54, 182
BfaI CTAG 3 cut(s) 953, 1017, 1331
BfmI CTRYAG 2 cut(s) 6, 456
BfuAI ACCTGC 1 cut(s) 1388
BisI GCNGC 3 cut(s) 252, 459, 1170
BlpI GCTNAGC 1 cut(s) 1042
BlsI GCNGC 3 cut(s) 253, 460, 1171
Bme1390I CCNGG 2 cut(s) 510, 649
BmiI GGNNCC 6 cut(s) 600, 645, 730, 763, 1006, 1234
BmrFI CCNGG 2 cut(s) 510, 649
BmrI ACTGGG 2 cut(s) 151, 749
BmsI GCATC 5 cut(s) 310, 1069, 1126, 1453, 1642
BmuI ACTGGG 2 cut(s) 151, 749
BpiI GAAGAC 4 cut(s) 89, 138, 335, 1569
Bpu10I CCTNAGC 2 cut(s) 1440, 1557
Bpu1102I GCTNAGC 1 cut(s) 1042
BpuEI CTTGAG 3 cut(s) 1567, 1643, 1658
BsaAI YACGTR 1 cut(s) 1285
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 56, 246, 647
Bsc4I CCNNNNNNNGG 6 cut(s) 535, 703, 1127, 1193, 1229, 1663
Bse1I ACTGG 6 cut(s) 157, 367, 419, 755, 1028, 1423
Bse3DI GCAATG 1 cut(s) 1070
BseBI CCWGG 2 cut(s) 510, 649
BseDI CCNNGG 3 cut(s) 56, 246, 647
BseGI GGATG 6 cut(s) 66, 423, 934, 1363, 1444, 1594
BseLI CCNNNNNNNGG 6 cut(s) 535, 703, 1127, 1193, 1229, 1663
BseMI GCAATG 1 cut(s) 1070
BseMII CTCAG 7 cut(s) 65, 562, 617, 1026, 1056, 1431, 1571
BseNI ACTGG 6 cut(s) 157, 367, 419, 755, 1028, 1423
BseRI GAGGAG 2 cut(s) 96, 181
BseSI GKGCMC 1 cut(s) 648
BseXI GCAGC 3 cut(s) 238, 470, 1156
BseYI CCCAGC 3 cut(s) 79, 590, 1193
BsgI GTGCAG 1 cut(s) 291
BshFI GGCC 3 cut(s) 61, 513, 977
BshNI GGYRCC 3 cut(s) 643, 728, 761
BsiHKAI GWGCWC 1 cut(s) 547
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 2 cut(s) 491, 719
BslI CCNNNNNNNGG 6 cut(s) 535, 703, 1127, 1193, 1229, 1663
BsmAI GTCTC 2 cut(s) 54, 182
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 491, 719
BsmI GAATGC 2 cut(s) 1202, 1297
BsnI GGCC 3 cut(s) 61, 513, 977
Bso31I GGTCTC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 547, 648
Bsp1720I GCTNAGC 1 cut(s) 1042
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 2 cut(s) 697, 1106
BspANI GGCC 3 cut(s) 61, 513, 977
BspCNI CTCAG 7 cut(s) 64, 561, 616, 1025, 1055, 1432, 1570
BspHI TCATGA 1 cut(s) 1212
BspLI GGNNCC 6 cut(s) 600, 645, 730, 763, 1006, 1234
BspMAI CTGCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 1388
BspPI GGATC 7 cut(s) 681, 1214, 1227, 1240, 1285, 1325, 1472
BspT107I GGYRCC 3 cut(s) 643, 728, 761
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 1070
BsrI ACTGG 6 cut(s) 157, 367, 419, 755, 1028, 1423
BssECI CCNNGG 3 cut(s) 56, 246, 647
BssT1I CCWWGG 2 cut(s) 56, 246
Bst2UI CCWGG 2 cut(s) 510, 649
Bst4CI ACNGT 6 cut(s) 32, 584, 616, 1349, 1414, 1609
Bst6I CTCTTC 1 cut(s) 1587
BstBAI YACGTR 1 cut(s) 1285
BstC8I GCNNGC 6 cut(s) 85, 270, 543, 627, 1200, 1341
BstDEI CTNAG 7 cut(s) 51, 548, 603, 1012, 1042, 1440, 1557
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 6 cut(s) 66, 423, 934, 1363, 1444, 1594
BstHHI GCGC 2 cut(s) 213, 566
BstMAI GTCTC 2 cut(s) 54, 182
BstMWI GCNNNNNNNGC 6 cut(s) 219, 251, 570, 1114, 1123, 1199
BstNI CCWGG 2 cut(s) 510, 649
BstNSI RCATGY 1 cut(s) 1343
BstSCI CCNGG 2 cut(s) 508, 647
BstSFI CTRYAG 2 cut(s) 6, 456
BstSLI GKGCMC 1 cut(s) 648
BstV1I GCAGC 3 cut(s) 238, 470, 1156
BstV2I GAAGAC 4 cut(s) 89, 138, 335, 1569
BstX2I RGATCY 1 cut(s) 1232
BstXI CCANNNNNNTGG 1 cut(s) 164
BstYI RGATCY 1 cut(s) 1232
BsuRI GGCC 3 cut(s) 61, 513, 977
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 6 cut(s) 66, 423, 934, 1363, 1444, 1594
BtsIMutI CAGTG 3 cut(s) 589, 899, 1614
BveI ACCTGC 1 cut(s) 1388
Cac8I GCNNGC 6 cut(s) 85, 270, 543, 627, 1200, 1341
CciI TCATGA 1 cut(s) 1212
CfoI GCGC 2 cut(s) 213, 566
CseI GACGC 1 cut(s) 164
Csp6I GTAC 4 cut(s) 275, 355, 554, 743
CviAII CATG 8 cut(s) 247, 392, 515, 709, 1213, 1340, 1693, 1714
CviQI GTAC 4 cut(s) 275, 355, 554, 743
DdeI CTNAG 7 cut(s) 51, 548, 603, 1012, 1042, 1440, 1557
DraI TTTAAA 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 1587
EarI CTCTTC 1 cut(s) 1587
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 2 cut(s) 56, 246
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 547
Eco31I GGTCTC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 857
EcoICRI GAGCTC 1 cut(s) 545
EcoRI GAATTC 2 cut(s) 1469, 1667
EcoRII CCWGG 2 cut(s) 508, 647
EcoT14I CCWWGG 2 cut(s) 56, 246
EcoT22I ATGCAT 1 cut(s) 1341
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 2 cut(s) 56, 246
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 8 cut(s) 250, 395, 518, 712, 1216, 1343, 1696, 1717
FaqI GGGAC 2 cut(s) 491, 719
FatI CATG 8 cut(s) 246, 391, 514, 708, 1212, 1339, 1692, 1713
FblI GTMKAC 1 cut(s) 1324
Fnu4HI GCNGC 3 cut(s) 252, 459, 1170
FokI GGATG 6 cut(s) 53, 430, 941, 1370, 1431, 1601
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 3 cut(s) 252, 459, 1170
FspBI CTAG 3 cut(s) 953, 1017, 1331
GlaI GCGC 2 cut(s) 212, 565
GluI GCNGC 3 cut(s) 252, 459, 1170
GsaI CCCAGC 3 cut(s) 83, 594, 1197
HaeIII GGCC 3 cut(s) 61, 513, 977
HapII CCGG 1 cut(s) 223
HgaI GACGC 1 cut(s) 164
HhaI GCGC 2 cut(s) 213, 566
Hin1II CATG 8 cut(s) 250, 395, 518, 712, 1216, 1343, 1696, 1717
Hin6I GCGC 2 cut(s) 211, 564
HinP1I GCGC 2 cut(s) 211, 564
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 3 cut(s) 11, 169, 1251
HpaII CCGG 1 cut(s) 223
HphI GGTGA 7 cut(s) 130, 172, 517, 1095, 1391, 1433, 1637
Hpy166II GTNNAC 4 cut(s) 28, 520, 664, 1325
Hpy188I TCNGA 5 cut(s) 20, 294, 610, 868, 1605
Hpy188III TCNNGA 9 cut(s) 822, 875, 953, 1207, 1213, 1331, 1559, 1637, 1660
Hpy8I GTNNAC 4 cut(s) 28, 520, 664, 1325
HpyAV CCTTC 5 cut(s) 389, 950, 1110, 1578, 1610
HpyCH4III ACNGT 6 cut(s) 32, 584, 616, 1349, 1414, 1609
HpyCH4IV ACGT 2 cut(s) 1284, 1462
HpyF10VI GCNNNNNNNGC 6 cut(s) 219, 251, 570, 1114, 1123, 1199
HpyF3I CTNAG 7 cut(s) 51, 548, 603, 1012, 1042, 1440, 1557
HpySE526I ACGT 2 cut(s) 1284, 1462
Hsp92II CATG 8 cut(s) 250, 395, 518, 712, 1216, 1343, 1696, 1717
HspAI GCGC 2 cut(s) 211, 564
LmnI GCTCC 3 cut(s) 421, 538, 1535
Lsp1109I GCAGC 3 cut(s) 238, 470, 1156
LweI GCATC 5 cut(s) 310, 1069, 1126, 1453, 1642
MaeI CTAG 3 cut(s) 953, 1017, 1331
MaeII ACGT 2 cut(s) 1284, 1462
MaeIII GTNAC 4 cut(s) 715, 897, 1414, 1433
MfeI CAATTG 2 cut(s) 1158, 1497
MflI RGATCY 1 cut(s) 1232
MhlI GDGCHC 2 cut(s) 547, 648
MlsI TGGCCA 1 cut(s) 513
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 210, 906
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 1341
MscI TGGCCA 1 cut(s) 513
MseI TTAA 7 cut(s) 492, 777, 984, 1050, 1362, 1488, 1548
MslI CAYNNNNRTG 2 cut(s) 318, 713
Msp20I TGGCCA 1 cut(s) 513
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 2 cut(s) 510, 649
MunI CAATTG 2 cut(s) 1158, 1497
Mva1269I GAATGC 2 cut(s) 1202, 1297
MvaI CCWGG 2 cut(s) 510, 649
MwoI GCNNNNNNNGC 6 cut(s) 219, 251, 570, 1114, 1123, 1199
NcoI CCATGG 1 cut(s) 246
NlaIII CATG 8 cut(s) 250, 395, 518, 712, 1216, 1343, 1696, 1717
NlaIV GGNNCC 6 cut(s) 600, 645, 730, 763, 1006, 1234
NmuCI GTSAC 3 cut(s) 715, 897, 1433
NsiI ATGCAT 1 cut(s) 1341
NspI RCATGY 1 cut(s) 1343
PaeI GCATGC 1 cut(s) 1343
PagI TCATGA 1 cut(s) 1212
PceI AGGCCT 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PctI GAATGC 2 cut(s) 1202, 1297
PfeI GAWTC 2 cut(s) 169, 1251
PflMI CCANNNNNTGG 1 cut(s) 1193
PkrI GCNGC 3 cut(s) 253, 460, 1171
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 1285
Psp124BI GAGCTC 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 508, 647
PspFI CCCAGC 3 cut(s) 79, 590, 1193
PspGI CCWGG 2 cut(s) 508, 647
PspN4I GGNNCC 6 cut(s) 600, 645, 730, 763, 1006, 1234
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 1232
RsaI GTAC 4 cut(s) 276, 356, 555, 744
RsaNI GTAC 4 cut(s) 275, 355, 554, 743
RseI CAYNNNNRTG 2 cut(s) 318, 713
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 7 cut(s) 492, 777, 984, 1050, 1362, 1488, 1548
SatI GCNGC 3 cut(s) 252, 459, 1170
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 510, 649
SduI GDGCHC 2 cut(s) 547, 648
SfaNI GCATC 5 cut(s) 310, 1069, 1126, 1453, 1642
SfcI CTRYAG 2 cut(s) 6, 456
SmiMI CAYNNNNRTG 2 cut(s) 318, 713
SmlI CTYRAG 3 cut(s) 1582, 1637, 1658
SmoI CTYRAG 3 cut(s) 1582, 1637, 1658
SphI GCATGC 1 cut(s) 1343
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 2 cut(s) 697, 1106
SspI AATATT 1 cut(s) 298
SspMI CTAG 3 cut(s) 953, 1017, 1331
SstI GAGCTC 1 cut(s) 547
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 508, 647
StyI CCWWGG 2 cut(s) 56, 246
TaaI ACNGT 6 cut(s) 32, 584, 616, 1349, 1414, 1609
TaiI ACGT 2 cut(s) 1287, 1465
TaqI TCGA 2 cut(s) 531, 1614
TatI WGTACW 2 cut(s) 354, 742
TfiI GAWTC 2 cut(s) 169, 1251
Tru1I TTAA 7 cut(s) 492, 777, 984, 1050, 1362, 1488, 1548
Tru9I TTAA 7 cut(s) 492, 777, 984, 1050, 1362, 1488, 1548
TscAI CASTG 3 cut(s) 589, 906, 1614
TseFI GTSAC 3 cut(s) 715, 897, 1433
TseI GCWGC 3 cut(s) 251, 458, 1169
Tsp45I GTSAC 3 cut(s) 715, 897, 1433
TspDTI ATGAA 5 cut(s) 408, 948, 1197, 1605, 1702
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 3 cut(s) 589, 906, 1614
Van91I CCANNNNNTGG 1 cut(s) 1193
XapI RAATTY 3 cut(s) 721, 1469, 1667
XbaI TCTAGA 2 cut(s) 952, 1330
XceI RCATGY 1 cut(s) 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XmiI GTMKAC 1 cut(s) 1324
XspI CTAG 3 cut(s) 953, 1017, 1331
Zsp2I ATGCAT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.