MD04G1081800.v1.1

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010128
Physical Location & Seq
Forward (+)
11290842 .. 11294854
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1081800.v1.1.491

Sequence Viewer

Length: 2250 bp
ATGGCTACAGAGTCAGTGAGAGTGTACAGTTTTAGCTTCAATGGAGGTTCTCAGACTCGGCCGGCCATGTCTCCCTCCCCAGCTCGCTTTCTCGGTCTTCGCCATCGACCCTCTTCTTCCCTCACTTCGTCTTCGCTTTCCCACTTCTTTGGGAATGTTCGTATCAGCTCCCACTCCTCAAGGCTCTCGAATTTGCGCCAGCAGAGCCGAAGAAACCTCTCTGTTTTTGCCATGGCCGCAGAGGATGGAAAGCGTGCAGTAGCTTTAGAAGATTATCGCAACATAGGAATTATGGCTCACATAGATGCAGGGAAGACGACTACAACTGAAAGAATCTTGTTTTATACTGGAAGAAACTATAAAATAGGGGAGGTACATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCTGCAACTACAACATTTTGGGACAATCACAGGATTAACATCATCGACACTCCTGGCCACGTTGACTTCACCCTTGAAGTGGAGCGGGCTCTAAGGGTGCTTGATGGTGCTATATGCTTGTTTGATAGTGTTGCTGGTGTGGAACCTCAATCTGAAACTGTGTGGAGGCAAGCTGATAGATATGGAGTGCCCAGGATTTGCTTTGTCAATAAAATGGATCGTCTTGGAGCAAACTTCTTCCGAACAAGAGACATGATGAGAGCTATACTTTGGTCTGGAGAAGAGTTGGGTGCTAAATTTGTTTACGAGGAGATTCCATCTCATCTTCTGGAGCTGGCTCAAGAATACAGGGCTGAGATGATAGAAACCATAGTTGAGTTGGATGATCAAGCAATGGAGGGCTATCTAGAAGGAGTTGAACCTGATGAGGAAACCATTAAGAAATTAATTAGGAAGGGAACCATCGCAATTAGTTTTGTGCCAGTATTATGTGGTTCGGCTTTTAAAAATAAGGGGGTTCAACCATTGCTTGATGCAGTTGTGGATTATTTGCCTTCACCACTTGATGTGCCACCAATGAAGGGGACTGATGCAGACAACCCTGAAATCACGATTGAAAGGGCTGCAAGTGATGAGGAACCATTTGCTGGGCTAGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATCTATGCAGGAAAGCTAACGGCTGGATCCTATGTTTTAAATGCAAACAAGGGAAAGAAGGAGAGGATTGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTAAAGGTAGCTTTAGCTGGTGATATCGTTGCACTTGCAGGCCTGAAGGATACCATTACCGGTGAAACATTGAGTGACCCCGATCATCCTATAGTGCTTGAACGAATGGACTTCCCTGATCCTGTAATTAAGGTTGCCATTGAACCCAAAACTAAAGCTGATGTTGATAAGATGGGAGCTGGTTTGGTTAAGCTTGCTCAAGAAGACCCTTCTTTCCACTTCTCTCGGGATGAAGAGATCAACCAGACAGTGATTGAAGGAATGGGAGAATTGCATCTTGAGATTATTGTTGATCGGCTCAAGAGAGAATTCAAGGTTGAAGCAAATGTTGGTGCACCCCAGGTAAACTACCGGGAAAGCATTTCGAGAACCTCCGAAGTGAGGTATGTGCACAAGAAACAATCAGGTGGACAAGGTCAATTTGCAGATGTCACCGTACGGTTTGAACCAATGGAGGCTGGCGGTGGATATGAGTTTAAGAGTGAAATCAAGGGAGGTGCAGTGCCAAGAGAATACATTCCTGGGGTCATGAAAGGATTAGAGGAGTGTATGAGCAACGGTGTGCTCGCAGGCTTTCCTGTTGTTGATGTACGCGCTGTACTAGTTGATGGTTCTTACCATGATGTCGACTCAAGTGTGTTAGCATTTCAATTGGCAGCAAGAGGAGCTTTCCGGGAGGGGATAAAGAAAGCTGCCCCGAAAATGCTTGAACCAATAATGAAAGTTGAAGTTGTTACACCAGAAGAACATCTCGGAGATGTGATTGGTGATCTTAACTCGAGGAGAGGACAGATCAACAGCTTCAATGACAAACCTGGTGGTCTCAAGGTGGTGGATTCACTGGTTCCTCTTGCGGAGATGTTTCAGTACGTGAGTACGCTGAGGGGCATGACGAAAGGTCGTGCATCGTATACAATGCAGTTAGCTAAGTTTGACGTTGTTCCTCAGCACATTCAGAACCAGCTTGCTGCCAAGGAGGAAGAAGTTGCTGCTTGA

Protein Analysis

750

Amino Acids

82.76

Weight (kDa)

5.44

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 91 - 333 2e-66 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 361 - 443 1.8e-16 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 376 - 442 1.5e-16 Elongation factor Tu domain 2
EFG_III PF14492 456 - 529 1.2e-31 Elongation Factor G, domain III
RF3_C PF16658 466 - 544 2.3e-06 Class II release factor RF3, C-terminal domain
EFG_IV PF03764 531 - 649 1.4e-45 Elongation factor G, domain IV
EFG_C PF00679 653 - 739 4.8e-26 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1088
AccBSI CCGCTC 1 cut(s) 526
AccI GTMKAC 3 cut(s) 1219, 1881, 2165
AccII CGCG 1 cut(s) 1848
AciI CCGC 4 cut(s) 237, 526, 1716, 2108
AclWI GGATC 7 cut(s) 666, 1109, 1122, 1135, 1167, 1180, 1367
AcoI YGGCCR 4 cut(s) 59, 63, 234, 496
AcsI RAATTY 3 cut(s) 190, 737, 1562
AcuI CTGAAG 1 cut(s) 1319
AfaI GTAC 7 cut(s) 26, 375, 1692, 1845, 1854, 2123, 2131
AfiI CCNNNNNNNGG 5 cut(s) 520, 1022, 1088, 1124, 1635
AgeI ACCGGT 1 cut(s) 1313
AhdI GACNNNNNGTC 1 cut(s) 2151
AhlI ACTAGT 1 cut(s) 1855
AjnI CCWGG 5 cut(s) 493, 632, 1593, 1774, 2068
AjuI GAANNNNNNNTTGG 2 cut(s) 1566, 1598
Alw21I GWGCWC 3 cut(s) 1591, 1647, 1821
Alw26I GTCTC 3 cut(s) 75, 684, 2081
Alw44I GTGCAC 2 cut(s) 1587, 1643
AlwI GGATC 7 cut(s) 666, 1109, 1122, 1135, 1167, 1180, 1367
Ama87I CYCGRG 2 cut(s) 1479, 2032
AoxI GGCC 5 cut(s) 59, 63, 234, 496, 1294
ApaLI GTGCAC 2 cut(s) 1587, 1643
ApeKI GCWGC 6 cut(s) 443, 1064, 1910, 1946, 2222, 2243
ApoI RAATTY 3 cut(s) 190, 737, 1562
AseI ATTAAT 1 cut(s) 887
AsiGI ACCGGT 1 cut(s) 1313
Asp700I GAANNNNTTC 1 cut(s) 1770
AspLEI GCGC 2 cut(s) 198, 1850
AsuC2I CCSGG 2 cut(s) 1607, 1928
AsuHPI GGTGA 6 cut(s) 502, 990, 1286, 1328, 1678, 2033
AsuNHI GCTAGC 1 cut(s) 1093
AvaI CYCGRG 2 cut(s) 1479, 2032
BaeGI GKGCMC 3 cut(s) 633, 1591, 1647
BalI TGGCCA 1 cut(s) 498
BamHI GGATCC 2 cut(s) 1127, 1172
BanII GRGCYC 1 cut(s) 532
BbsI GAAGAC 4 cut(s) 89, 123, 320, 1464
Bbv12I GWGCWC 3 cut(s) 1591, 1647, 1821
BbvCI CCTCAGC 2 cut(s) 2135, 2199
BbvI GCAGC 6 cut(s) 430, 1051, 1922, 1933, 2209, 2230
BccI CCATC 8 cut(s) 111, 239, 397, 539, 766, 911, 1420, 1856
BceAI ACGGC 1 cut(s) 1182
BciT130I CCWGG 5 cut(s) 495, 634, 1595, 1776, 2070
BciVI GTATCC 1 cut(s) 1297
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 2 cut(s) 1607, 1928
BcoDI GTCTC 3 cut(s) 75, 684, 2081
BcuI ACTAGT 1 cut(s) 1855
BfaI CTAG 4 cut(s) 848, 1094, 1226, 1856
BfmI CTRYAG 2 cut(s) 6, 1344
BfuI GTATCC 1 cut(s) 1297
BisI GCNGC 7 cut(s) 237, 444, 1065, 1911, 1947, 2223, 2244
BlsI GCNGC 7 cut(s) 238, 445, 1066, 1912, 1948, 2224, 2245
Bme1390I CCNGG 7 cut(s) 495, 634, 1595, 1607, 1776, 1928, 2070
BmeRI GACNNNNNGTC 1 cut(s) 2151
BmeT110I CYCGRG 2 cut(s) 1479, 2032
BmiI GGNNCC 6 cut(s) 585, 901, 1080, 1129, 1174, 2100
BmrFI CCNGG 7 cut(s) 495, 634, 1595, 1607, 1776, 1928, 2070
BmsI GCATC 5 cut(s) 295, 964, 1021, 1537, 2168
BmtI GCTAGC 1 cut(s) 1097
BpiI GAAGAC 4 cut(s) 89, 123, 320, 1464
BpmI CTGGAG 2 cut(s) 738, 791
Bpu10I CCTNAGC 2 cut(s) 2135, 2199
BpuEI CTTGAG 7 cut(s) 163, 765, 1437, 1538, 1553, 1870, 2063
BpuMI CCSGG 2 cut(s) 1607, 1928
BsaAI YACGTR 1 cut(s) 2125
BsaBI GATNNNNATC 1 cut(s) 479
BsaI GGTCTC 1 cut(s) 2081
BsaJI CCNNGG 5 cut(s) 231, 632, 1593, 1775, 2226
BsaWI WCCGGW 1 cut(s) 1313
BsaXI ACNNNNNCTCC 2 cut(s) 1740, 1770
Bsc4I CCNNNNNNNGG 5 cut(s) 520, 1022, 1088, 1124, 1635
Bse118I RCCGGY 2 cut(s) 61, 1313
Bse1I ACTGG 4 cut(s) 352, 404, 923, 2100
Bse3DI GCAATG 2 cut(s) 840, 965
Bse8I GATNNNNATC 1 cut(s) 479
BseBI CCWGG 5 cut(s) 495, 634, 1595, 1776, 2070
BseDI CCNNGG 5 cut(s) 231, 632, 1593, 1775, 2226
BseGI GGATG 6 cut(s) 250, 408, 829, 1258, 1339, 1489
BseJI GATNNNNATC 1 cut(s) 479
BseLI CCNNNNNNNGG 5 cut(s) 520, 1022, 1088, 1124, 1635
BseMI GCAATG 2 cut(s) 840, 965
BseMII CTCAG 4 cut(s) 65, 786, 2126, 2213
BseNI ACTGG 4 cut(s) 352, 404, 923, 2100
BseRI GAGGAG 5 cut(s) 166, 764, 1811, 1932, 2050
BseSI GKGCMC 3 cut(s) 633, 1591, 1647
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 6 cut(s) 430, 1051, 1922, 1933, 2209, 2230
BseYI CCCAGC 2 cut(s) 79, 1088
BsgI GTGCAG 2 cut(s) 276, 1773
Bsh1236I CGCG 1 cut(s) 1848
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 5 cut(s) 61, 65, 236, 498, 1296
BshTI ACCGGT 1 cut(s) 1313
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 3 cut(s) 1591, 1647, 1821
BsiHKCI CYCGRG 2 cut(s) 1479, 2032
BsiSI CCGG 4 cut(s) 62, 1314, 1606, 1927
BsiWI CGTACG 1 cut(s) 1690
BslFI GGGAC 2 cut(s) 476, 1039
BslI CCNNNNNNNGG 5 cut(s) 520, 1022, 1088, 1124, 1635
BsmAI GTCTC 3 cut(s) 75, 684, 2081
BsmFI GGGAC 2 cut(s) 476, 1039
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 5 cut(s) 61, 65, 236, 498, 1296
Bso31I GGTCTC 1 cut(s) 2081
BsoBI CYCGRG 2 cut(s) 1479, 2032
Bsp1286I GDGCHC 5 cut(s) 532, 633, 1591, 1647, 1821
Bsp1407I TGTACA 1 cut(s) 24
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 4 cut(s) 237, 526, 1716, 2108
BspANI GGCC 5 cut(s) 61, 65, 236, 498, 1296
BspCNI CTCAG 4 cut(s) 64, 787, 2127, 2212
BspFNI CGCG 1 cut(s) 1848
BspHI TCATGA 2 cut(s) 1107, 1782
BspLI GGNNCC 6 cut(s) 585, 901, 1080, 1129, 1174, 2100
BspOI GCTAGC 1 cut(s) 1097
BspPI GGATC 7 cut(s) 666, 1109, 1122, 1135, 1167, 1180, 1367
BspTNI GGTCTC 1 cut(s) 2081
BsrBI CCGCTC 1 cut(s) 526
BsrDI GCAATG 2 cut(s) 840, 965
BsrFI RCCGGY 2 cut(s) 61, 1313
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 4 cut(s) 352, 404, 923, 2100
BssAI RCCGGY 2 cut(s) 61, 1313
BssECI CCNNGG 5 cut(s) 231, 632, 1593, 1775, 2226
BssNAI GTATAC 1 cut(s) 2166
BssT1I CCWWGG 2 cut(s) 231, 2226
Bst1107I GTATAC 1 cut(s) 2166
Bst2UI CCWGG 5 cut(s) 495, 634, 1595, 1776, 2070
Bst4CI ACNGT 7 cut(s) 29, 601, 1244, 1504, 1690, 1695, 1814
Bst6I CTCTTC 3 cut(s) 118, 717, 1482
BstAUI TGTACA 1 cut(s) 24
BstBAI YACGTR 1 cut(s) 2125
BstDEI CTNAG 6 cut(s) 51, 533, 795, 2135, 2181, 2199
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 6 cut(s) 250, 408, 829, 1258, 1339, 1489
BstFNI CGCG 1 cut(s) 1848
BstHHI GCGC 2 cut(s) 198, 1850
BstMAI GTCTC 3 cut(s) 75, 684, 2081
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 4 cut(s) 204, 236, 1094, 1919
BstNI CCWGG 5 cut(s) 495, 634, 1595, 1776, 2070
BstNSI RCATGY 1 cut(s) 1238
BstSCI CCNGG 7 cut(s) 493, 632, 1593, 1605, 1774, 1926, 2068
BstSFI CTRYAG 2 cut(s) 6, 1344
BstSLI GKGCMC 3 cut(s) 633, 1591, 1647
BstUI CGCG 1 cut(s) 1848
BstV1I GCAGC 6 cut(s) 430, 1051, 1922, 1933, 2209, 2230
BstV2I GAAGAC 4 cut(s) 89, 123, 320, 1464
BstX2I RGATCY 2 cut(s) 1127, 1172
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 2 cut(s) 1127, 1172
BstZ17I GTATAC 1 cut(s) 2166
BstZI CGGCCG 1 cut(s) 59
BsuI GTATCC 1 cut(s) 1297
BsuRI GGCC 5 cut(s) 61, 65, 236, 498, 1296
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 889
BtsCI GGATG 6 cut(s) 250, 408, 829, 1258, 1339, 1489
BtsI GCAGTG 1 cut(s) 1761
BtsIMutI CAGTG 4 cut(s) 21, 1509, 1761, 2093
CciI TCATGA 2 cut(s) 1107, 1782
CfoI GCGC 2 cut(s) 198, 1850
Cfr10I RCCGGY 2 cut(s) 61, 1313
CsiI ACCWGGT 1 cut(s) 2068
Csp6I GTAC 7 cut(s) 25, 374, 1691, 1844, 1853, 2122, 2130
CspAI ACCGGT 1 cut(s) 1313
CspCI CAANNNNNGTGG 2 cut(s) 2053, 2088
CviAII CATG 9 cut(s) 67, 232, 377, 694, 1108, 1235, 1783, 1874, 2143
CviQI GTAC 7 cut(s) 25, 374, 1691, 1844, 1853, 2122, 2130
DdeI CTNAG 6 cut(s) 51, 533, 795, 2135, 2181, 2199
DraI TTTAAA 2 cut(s) 946, 1185
DriI GACNNNNNGTC 1 cut(s) 2151
EaeI YGGCCR 4 cut(s) 59, 63, 234, 496
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 3 cut(s) 118, 717, 1482
Eam1105I GACNNNNNGTC 1 cut(s) 2151
EarI CTCTTC 3 cut(s) 118, 717, 1482
EclXI CGGCCG 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 231, 2226
Eco147I AGGCCT 1 cut(s) 1296
Eco24I GRGCYC 1 cut(s) 532
Eco31I GGTCTC 1 cut(s) 2081
Eco32I GATATC 1 cut(s) 1279
Eco52I CGGCCG 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 1319
Eco88I CYCGRG 2 cut(s) 1479, 2032
EcoRI GAATTC 1 cut(s) 1562
EcoRII CCWGG 5 cut(s) 493, 632, 1593, 1774, 2068
EcoRV GATATC 1 cut(s) 1279
EcoT14I CCWWGG 2 cut(s) 231, 2226
EcoT22I ATGCAT 1 cut(s) 1236
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 2 cut(s) 231, 2226
FaeI CATG 9 cut(s) 70, 235, 380, 697, 1111, 1238, 1786, 1877, 2146
FalI AAGNNNNNCTT 2 cut(s) 1906, 1938
FaqI GGGAC 2 cut(s) 476, 1039
FatI CATG 9 cut(s) 66, 231, 376, 693, 1107, 1234, 1782, 1873, 2142
FauI CCCGC 1 cut(s) 519
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 3 cut(s) 1219, 1881, 2165
Fnu4HI GCNGC 7 cut(s) 237, 444, 1065, 1911, 1947, 2223, 2244
FokI GGATG 6 cut(s) 257, 415, 836, 1265, 1326, 1496
FriOI GRGCYC 1 cut(s) 532
FseI GGCCGGCC 1 cut(s) 65
Fsp4HI GCNGC 7 cut(s) 237, 444, 1065, 1911, 1947, 2223, 2244
FspBI CTAG 4 cut(s) 848, 1094, 1226, 1856
GlaI GCGC 2 cut(s) 197, 1849
GluI GCNGC 7 cut(s) 237, 444, 1065, 1911, 1947, 2223, 2244
GsaI CCCAGC 2 cut(s) 83, 1092
GsuI CTGGAG 2 cut(s) 738, 791
HaeIII GGCC 5 cut(s) 61, 65, 236, 498, 1296
HapII CCGG 4 cut(s) 62, 1314, 1606, 1927
HhaI GCGC 2 cut(s) 198, 1850
Hin1II CATG 9 cut(s) 70, 235, 380, 697, 1111, 1238, 1786, 1877, 2146
Hin6I GCGC 2 cut(s) 196, 1848
HinP1I GCGC 2 cut(s) 196, 1848
HincII GTYRAC 2 cut(s) 505, 1882
HindII GTYRAC 2 cut(s) 505, 1882
HindIII AAGCTT 1 cut(s) 1445
HinfI GANTC 7 cut(s) 11, 55, 333, 754, 1146, 1883, 2090
HpaII CCGG 4 cut(s) 62, 1314, 1606, 1927
HphI GGTGA 6 cut(s) 502, 990, 1286, 1328, 1678, 2033
Hpy188I TCNGA 6 cut(s) 54, 595, 683, 1630, 2009, 2211
HpyAV CCTTC 9 cut(s) 374, 845, 889, 1005, 1015, 1198, 1294, 1473, 1505
HpyCH4III ACNGT 7 cut(s) 29, 601, 1244, 1504, 1690, 1695, 1814
HpyCH4IV ACGT 3 cut(s) 501, 2124, 2190
HpyF10VI GCNNNNNNNGC 4 cut(s) 204, 236, 1094, 1919
HpyF3I CTNAG 6 cut(s) 51, 533, 795, 2135, 2181, 2199
HpySE526I ACGT 3 cut(s) 501, 2124, 2190
Hsp92II CATG 9 cut(s) 70, 235, 380, 697, 1111, 1238, 1786, 1877, 2146
HspAI GCGC 2 cut(s) 196, 1848
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 826
LmnI GCTCC 7 cut(s) 173, 406, 523, 668, 772, 1430, 1919
Lsp1109I GCAGC 6 cut(s) 430, 1051, 1922, 1933, 2209, 2230
LweI GCATC 5 cut(s) 295, 964, 1021, 1537, 2168
MabI ACCWGGT 1 cut(s) 2068
MaeI CTAG 4 cut(s) 848, 1094, 1226, 1856
MaeII ACGT 3 cut(s) 501, 2124, 2190
MaeIII GTNAC 3 cut(s) 1328, 1684, 1987
MbiI CCGCTC 1 cut(s) 526
MfeI CAATTG 1 cut(s) 1904
MflI RGATCY 2 cut(s) 1127, 1172
MhlI GDGCHC 5 cut(s) 532, 633, 1591, 1647, 1821
MlsI TGGCCA 1 cut(s) 498
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 3 cut(s) 20, 49, 1877
MmeI TCCRAC 1 cut(s) 801
Mox20I TGGCCA 1 cut(s) 498
Mph1103I ATGCAT 1 cut(s) 1236
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 1770
MscI TGGCCA 1 cut(s) 498
MseI TTAA 9 cut(s) 477, 879, 887, 945, 1184, 1383, 1443, 1731, 2028
MslI CAYNNNNRTG 1 cut(s) 303
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 4 cut(s) 62, 1314, 1606, 1927
MspR9I CCNGG 7 cut(s) 495, 634, 1595, 1607, 1776, 1928, 2070
MunI CAATTG 1 cut(s) 1904
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 5 cut(s) 495, 634, 1595, 1776, 2070
MvnI CGCG 1 cut(s) 1848
MwoI GCNNNNNNNGC 4 cut(s) 204, 236, 1094, 1919
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 1607, 1928
NcoI CCATGG 1 cut(s) 231
NgoMIV GCCGGC 1 cut(s) 61
NheI GCTAGC 1 cut(s) 1093
NlaIII CATG 9 cut(s) 70, 235, 380, 697, 1111, 1238, 1786, 1877, 2146
NlaIV GGNNCC 6 cut(s) 585, 901, 1080, 1129, 1174, 2100
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 2 cut(s) 1328, 1684
NsiI ATGCAT 1 cut(s) 1236
NspI RCATGY 1 cut(s) 1238
PaeI GCATGC 1 cut(s) 1238
PaeR7I CTCGAG 1 cut(s) 2032
PagI TCATGA 2 cut(s) 1107, 1782
PceI AGGCCT 1 cut(s) 1296
PctI GAATGC 1 cut(s) 1097
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 1770
PfeI GAWTC 4 cut(s) 333, 754, 1146, 2090
Pfl23II CGTACG 1 cut(s) 1690
PflFI GACNNNGTC 1 cut(s) 1668
PflMI CCANNNNNTGG 1 cut(s) 1088
PfoI TCCNGGA 1 cut(s) 1926
PinAI ACCGGT 1 cut(s) 1313
PkrI GCNGC 7 cut(s) 238, 445, 1066, 1912, 1948, 2224, 2245
PleI GAGTC 3 cut(s) 19, 49, 1877
PpsI GAGTC 3 cut(s) 19, 49, 1877
Ppu21I YACGTR 1 cut(s) 2125
PshBI ATTAAT 1 cut(s) 887
Psp6I CCWGG 5 cut(s) 493, 632, 1593, 1774, 2068
PspFI CCCAGC 2 cut(s) 79, 1088
PspGI CCWGG 5 cut(s) 493, 632, 1593, 1774, 2068
PspLI CGTACG 1 cut(s) 1690
PspN4I GGNNCC 6 cut(s) 585, 901, 1080, 1129, 1174, 2100
PspXI VCTCGAGB 1 cut(s) 2032
PsuI RGATCY 2 cut(s) 1127, 1172
PsyI GACNNNGTC 1 cut(s) 1668
RigI GGCCGGCC 1 cut(s) 65
RsaI GTAC 7 cut(s) 26, 375, 1692, 1845, 1854, 2123, 2131
RsaNI GTAC 7 cut(s) 25, 374, 1691, 1844, 1853, 2122, 2130
RseI CAYNNNNRTG 1 cut(s) 303
SalI GTCGAC 1 cut(s) 1880
SaqAI TTAA 9 cut(s) 477, 879, 887, 945, 1184, 1383, 1443, 1731, 2028
SatI GCNGC 7 cut(s) 237, 444, 1065, 1911, 1947, 2223, 2244
SchI GAGTC 3 cut(s) 20, 49, 1877
ScrFI CCNGG 7 cut(s) 495, 634, 1595, 1607, 1776, 1928, 2070
SduI GDGCHC 5 cut(s) 532, 633, 1591, 1647, 1821
SexAI ACCWGGT 1 cut(s) 2068
SfaNI GCATC 5 cut(s) 295, 964, 1021, 1537, 2168
SfcI CTRYAG 2 cut(s) 6, 1344
Sfr274I CTCGAG 1 cut(s) 2032
SlaI CTCGAG 1 cut(s) 2032
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 8 cut(s) 178, 780, 1452, 1532, 1553, 1885, 2032, 2078
SmoI CTYRAG 8 cut(s) 178, 780, 1452, 1532, 1553, 1885, 2032, 2078
SpeI ACTAGT 1 cut(s) 1855
SphI GCATGC 1 cut(s) 1238
SseBI AGGCCT 1 cut(s) 1296
SsiI CCGC 4 cut(s) 237, 526, 1716, 2108
SspMI CTAG 4 cut(s) 848, 1094, 1226, 1856
StuI AGGCCT 1 cut(s) 1296
StyD4I CCNGG 7 cut(s) 493, 632, 1593, 1605, 1774, 1926, 2068
StyI CCWWGG 2 cut(s) 231, 2226
TaaI ACNGT 7 cut(s) 29, 601, 1244, 1504, 1690, 1695, 1814
TaiI ACGT 3 cut(s) 504, 2127, 2193
TaqI TCGA 6 cut(s) 106, 188, 486, 1619, 1881, 2033
TaqII GACCGA 1 cut(s) 83
TatI WGTACW 2 cut(s) 24, 1852
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 4 cut(s) 333, 754, 1146, 2090
Tru1I TTAA 9 cut(s) 477, 879, 887, 945, 1184, 1383, 1443, 1731, 2028
Tru9I TTAA 9 cut(s) 477, 879, 887, 945, 1184, 1383, 1443, 1731, 2028
TscAI CASTG 4 cut(s) 21, 1509, 1761, 2100
TseFI GTSAC 2 cut(s) 1328, 1684
TseI GCWGC 6 cut(s) 443, 1064, 1910, 1946, 2222, 2243
Tsp45I GTSAC 2 cut(s) 1328, 1684
TspDTI ATGAA 5 cut(s) 393, 1034, 1500, 1799, 1988
TspRI CASTG 4 cut(s) 21, 1509, 1761, 2100
Tth111I GACNNNGTC 1 cut(s) 1668
Van91I CCANNNNNTGG 1 cut(s) 1088
VneI GTGCAC 2 cut(s) 1587, 1643
VspI ATTAAT 1 cut(s) 887
XapI RAATTY 3 cut(s) 190, 737, 1562
XbaI TCTAGA 2 cut(s) 847, 1225
XceI RCATGY 1 cut(s) 1238
XcmI CCANNNNNNNNNTGG 1 cut(s) 817
XhoI CTCGAG 1 cut(s) 2032
XmiI GTMKAC 3 cut(s) 1219, 1881, 2165
XmnI GAANNNNTTC 1 cut(s) 1770
XspI CTAG 4 cut(s) 848, 1094, 1226, 1856
Zsp2I ATGCAT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.