Rw7G017130

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010128
Physical Location & Seq
Forward (+)
17779497 .. 17783210
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G017130.1

Sequence Viewer

Length: 2352 bp
ATGGCTACAGAGTCAGTCAGAGTAGCCCACAGTTTCAGCTTCAATGGGTCTCAGACAAGGCCTACCATCCCTCTCTCCCCAGCTCGCTTTCTGGGTCTTGGTCCTCCTCGTTCTTCTTCTTCTTCTTCTTCTCTCACCTCGTCTTCACTTTCCCAGTTCTTTGGGAATCTGCGTCTCTCCTCCAACTCCTCAAAGGCCTGTCATTTGCGCCAACACAGCCGAAGAAACCTCTCTGTTTTCGCCATGGCCGCCGATGATGGAAAGCGTGCAGTACCATTGGTAGATTATCGGAATATTGGAATTATGGCTCACATAGATGCAGGGAAGACTACTACAACTGAACGGATTTTGTACTATACTGGAAGAAACTATAAAATTGGGGAGGTGCATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCAGCAACTACAACATTTTGGGACAAACACAGAATTAACATTATTGATACTCCTGGCCATGTGGACTTCACCCTCGAAGTGGAGCGAGCTCTCAGGGTACTTGATGGCGCAATATGCTTGTTTGACAGTGTTGCTGGGGTGGAACCTCAGTCTGAAACTGTGTGGAGGCAAGCTGATAAATATGGGGTGCCCAGGATTTGCTTTGTCAACAAAATGGATCGTCTTGGGGCAAACTTTTTCCGCACAAGGGACATGATAGTGACAAATTTGGGTGCCAAACCACTTGTACTTCAAATTCCAGTTGGTGCCGAAGATAATTTTAAGGGAGTTATTGATCTTGTGAAGATGAGAGCTATACTTTGGTCAGGAGAAGAATTGGGTGCAAAGTTTTCGTATGAGGATATTCCATCTGATCTTCAGGAGTTGGCTGACGAATACAGGTCACTGATGATAGAAACCATAGTTGAGTTGGATGATGAAGCTATGGAGGGCTATCTAGAAGGAGTTGAACCTGATGAGGCCACCATTAAGAAATTGATTAGGCAGGGAACCATCTCAGCTAGTTTTGTGCCAGTATTATGTGGCTCAGCTTTTAAAAATAAGGGGGTTCAACCATTGCTCGATGCTGTCGTGGATTATTTACCTTCACCGCTTGATGTGCCACCAATGCAGGGAACTGATGCAGATAACCCAGAAATCACGATTGAGAGGGCTGCAAGTGATGATGAACCATTTGCTGGGCTGGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATCTATGCAGGAAAGCTAACTGCTGGATCATACGTGCTGAATGCAAACAAGGGGAAAAAGGAGAGGATCGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTTAAGGTTGCTTTAGCTGGTGATATTGTTGCTCTTGCAGGTCTAAAAGATACTGTTACTGGTGAAACATTGAGTGACCCTGAGCATCCTATTGTGCTTGAACGTATGGAATTCCCTGATCCTGTGATTAAGGTGGCAATTGAACCCAAAACTAAAGCCGATGTTGATAAGATGGGAGCTGGTTTGGTTAAACTTGCTCAGGAAGACCCTTCATTCCACTTCTCAAGGGATGAAGAGGTCAATCAGACAGTGATCGAAGGAATGGGTGAATTGCATCTTGAGATTATTGTTGATCGCCTCAAGAGGGAATTCAAGGTCGAGGCAAATGTTGGAGCACCCCAGGTAAACTACCGTGAAAGCATTTCTAAAGTCGCAGAAGTAAAGTATGTGCACAAGAAACAATCTGGCGGACAAGGTCAATTCGCAGATATCACAATCCGATTTGAACCCATGGAGCCTGGTAATGGATACGAATTCAAGAGTGAAATCAAGGGAGGTGCAGTGCCAAGAGAGTACATTCCTGGCGTGATGAAAGGACTAGAGGAGTGTATGAGTAACGGTGTGCTTGCGGGCTTTCCTGTCGTCGACGTGCGTGCTGTACTAGTTGATGGTTCTTACCACGATGTTGACTCAAGTGTTTTGGCATTCCAGTTGGCAGCTAGAGGAGCTTTCCGAGAGGGTATGAAGAGAGCCGGCCCAAAAATGCTTGAACCTATTATGAAAGTTGAAGTAGTTACACCTGAAGAACATTTGGGAGATGTAATTGGTGACCTTAACTCCAGGAGAGGTCAAATCAACAGCTTTAGTGACAAACCTGGTGGTCTCAAGGTGGTTGATTCTTTGGTTCCTCTTGCGGAGATGTTCCAATATGTTAGTACACTCAGGGGCATGACAAAAGGTCGTGCATCCTACACGATGCAATTAGCTAGGTTTGATGTTGTTCCTCAGCACATTCAGAACCAACTTGCCGCTAAGGAGGAAGAAGTTGCCGCTTGA

Protein Analysis

783

Amino Acids

86.01

Weight (kDa)

5.32

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 96 - 367 2.3e-67 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 395 - 477 1.4e-16 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 410 - 476 1.8e-16 Elongation factor Tu domain 2
EFG_III PF14492 490 - 563 7.1e-32 Elongation Factor G, domain III
RF3_C PF16658 500 - 578 2.2e-06 Class II release factor RF3, C-terminal domain
EFG_IV PF03764 565 - 683 4.6e-46 Elongation factor G, domain IV
EFG_C PF00679 687 - 773 4.5e-26 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1385
AccB1I GGYRCC 3 cut(s) 640, 725, 758
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccI GTMKAC 2 cut(s) 1321, 1941
AciI CCGC 8 cut(s) 249, 694, 1103, 1764, 1925, 2210, 2325, 2346
AclWI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
AcoI YGGCCR 2 cut(s) 246, 508
AcsI RAATTY 5 cut(s) 718, 747, 1466, 1664, 1829
AcuI CTGAAG 2 cut(s) 854, 2118
AfaI GTAC 7 cut(s) 273, 353, 552, 741, 1871, 1956, 2233
AfiI CCNNNNNNNGG 7 cut(s) 532, 700, 1124, 1190, 1226, 1660, 1820
AhdI GACNNNNNGTC 1 cut(s) 2253
AhlI ACTAGT 1 cut(s) 1957
AjiI CACGTC 1 cut(s) 1945
AjnI CCWGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
Alw21I GWGCWC 3 cut(s) 544, 1693, 1749
Alw26I GTCTC 3 cut(s) 54, 179, 2183
Alw44I GTGCAC 1 cut(s) 1745
AlwI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
AoxI GGCC 6 cut(s) 59, 195, 246, 508, 972, 2050
ApaLI GTGCAC 1 cut(s) 1745
ApeKI GCWGC 3 cut(s) 455, 1166, 2012
ApoI RAATTY 5 cut(s) 718, 747, 1466, 1664, 1829
AspLEI GCGC 2 cut(s) 210, 563
AspS9I GGNCC 2 cut(s) 101, 2051
AsuHPI GGTGA 7 cut(s) 127, 514, 1092, 1388, 1430, 1634, 2135
AvaII GGWCC 1 cut(s) 101
BaeGI GKGCMC 2 cut(s) 645, 1749
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 1229
BanI GGYRCC 3 cut(s) 640, 725, 758
BanII GRGCYC 1 cut(s) 544
BbsI GAAGAC 3 cut(s) 135, 332, 1566
Bbv12I GWGCWC 3 cut(s) 544, 1693, 1749
BbvCI CCTCAGC 1 cut(s) 2301
BbvI GCAGC 3 cut(s) 467, 1153, 2024
BccI CCATC 8 cut(s) 74, 251, 409, 551, 868, 1013, 1522, 1958
BcgI CGANNNNNNTGC 4 cut(s) 825, 859, 1931, 1965
BciT130I CCWGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
BciVI GTATCC 1 cut(s) 1817
BcoDI GTCTC 3 cut(s) 54, 179, 2183
BcuI ACTAGT 1 cut(s) 1957
BfaI CTAG 7 cut(s) 950, 1014, 1328, 1895, 1958, 2016, 2283
BfmI CTRYAG 2 cut(s) 6, 453
BfuAI ACCTGC 1 cut(s) 1385
BfuI GTATCC 1 cut(s) 1817
BisI GCNGC 6 cut(s) 249, 456, 1167, 2013, 2325, 2346
BlpI GCTNAGC 1 cut(s) 1039
BlsI GCNGC 6 cut(s) 250, 457, 1168, 2014, 2326, 2347
Bme1390I CCNGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 2253
BmgBI CACGTC 1 cut(s) 1945
BmgT120I GGNCC 2 cut(s) 101, 2051
BmiI GGNNCC 8 cut(s) 597, 642, 727, 760, 1003, 1231, 1812, 2202
BmrFI CCNGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 7 cut(s) 307, 1066, 1123, 1450, 1639, 2262, 2270
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 3 cut(s) 135, 332, 1566
BpmI CTGGAG 1 cut(s) 2119
Bpu10I CCTNAGC 4 cut(s) 1437, 1554, 2301, 2328
Bpu1102I GCTNAGC 1 cut(s) 1039
BpuEI CTTGAG 5 cut(s) 1564, 1640, 1655, 1972, 2165
BsaAI YACGTR 1 cut(s) 1282
BsaI GGTCTC 2 cut(s) 54, 2183
BsaJI CCNNGG 4 cut(s) 243, 644, 1695, 1806
BsaXI ACNNNNNCTCC 4 cut(s) 1842, 1872, 2117, 2147
Bsc4I CCNNNNNNNGG 7 cut(s) 532, 700, 1124, 1190, 1226, 1660, 1820
Bse118I RCCGGY 1 cut(s) 2048
Bse1I ACTGG 7 cut(s) 154, 364, 416, 752, 1025, 1420, 2005
Bse3DI GCAATG 1 cut(s) 1067
BseBI CCWGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
BseDI CCNNGG 4 cut(s) 243, 644, 1695, 1806
BseGI GGATG 7 cut(s) 66, 420, 931, 1360, 1441, 1591, 2261
BseLI CCNNNNNNNGG 7 cut(s) 532, 700, 1124, 1190, 1226, 1660, 1820
BseMI GCAATG 1 cut(s) 1067
BseMII CTCAG 9 cut(s) 65, 559, 614, 1023, 1053, 1428, 1568, 2251, 2315
BseNI ACTGG 7 cut(s) 154, 364, 416, 752, 1025, 1420, 2005
BseRI GAGGAG 5 cut(s) 96, 169, 178, 1913, 2034
BseSI GKGCMC 2 cut(s) 645, 1749
BseXI GCAGC 3 cut(s) 467, 1153, 2024
BseYI CCCAGC 3 cut(s) 79, 587, 1190
BsgI GTGCAG 2 cut(s) 288, 1875
BshFI GGCC 6 cut(s) 61, 197, 248, 510, 974, 2052
BshNI GGYRCC 3 cut(s) 640, 725, 758
BsiHKAI GWGCWC 3 cut(s) 544, 1693, 1749
BsiSI CCGG 1 cut(s) 2049
BslFI GGGAC 2 cut(s) 488, 716
BslI CCNNNNNNNGG 7 cut(s) 532, 700, 1124, 1190, 1226, 1660, 1820
BsmAI GTCTC 3 cut(s) 54, 179, 2183
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 488, 716
BsmI GAATGC 3 cut(s) 1199, 1294, 2000
BsnI GGCC 6 cut(s) 61, 197, 248, 510, 974, 2052
Bso31I GGTCTC 2 cut(s) 54, 2183
Bsp1286I GDGCHC 4 cut(s) 544, 645, 1693, 1749
Bsp1720I GCTNAGC 1 cut(s) 1039
Bsp19I CCATGG 2 cut(s) 243, 1806
BspACI CCGC 8 cut(s) 249, 694, 1103, 1764, 1925, 2210, 2325, 2346
BspANI GGCC 6 cut(s) 61, 197, 248, 510, 974, 2052
BspCNI CTCAG 9 cut(s) 64, 558, 613, 1022, 1052, 1429, 1567, 2250, 2314
BspHI TCATGA 1 cut(s) 1209
BspLI GGNNCC 8 cut(s) 597, 642, 727, 760, 1003, 1231, 1812, 2202
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 1385
BspPI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
BspT107I GGYRCC 3 cut(s) 640, 725, 758
BspTNI GGTCTC 2 cut(s) 54, 2183
BsrDI GCAATG 1 cut(s) 1067
BsrFI RCCGGY 1 cut(s) 2048
BsrI ACTGG 7 cut(s) 154, 364, 416, 752, 1025, 1420, 2005
BssAI RCCGGY 1 cut(s) 2048
BssECI CCNNGG 4 cut(s) 243, 644, 1695, 1806
BssT1I CCWWGG 2 cut(s) 243, 1806
Bst2UI CCWGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
Bst4CI ACNGT 8 cut(s) 32, 581, 613, 1346, 1411, 1606, 1709, 1916
Bst6I CTCTTC 2 cut(s) 1584, 2036
BstBAI YACGTR 1 cut(s) 1282
BstDSI CCRYGG 2 cut(s) 243, 1806
BstEII GGTNACC 1 cut(s) 2123
BstF5I GGATG 7 cut(s) 66, 420, 931, 1360, 1441, 1591, 2261
BstHHI GCGC 2 cut(s) 210, 563
BstMAI GTCTC 3 cut(s) 54, 179, 2183
BstMWI GCNNNNNNNGC 7 cut(s) 216, 248, 567, 1111, 1120, 1196, 2021
BstNI CCWGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
BstNSI RCATGY 1 cut(s) 1340
BstPI GGTNACC 1 cut(s) 2123
BstSCI CCNGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
BstSFI CTRYAG 2 cut(s) 6, 453
BstSLI GKGCMC 2 cut(s) 645, 1749
BstV1I GCAGC 3 cut(s) 467, 1153, 2024
BstV2I GAAGAC 3 cut(s) 135, 332, 1566
BstX2I RGATCY 1 cut(s) 1229
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 1229
BsuI GTATCC 1 cut(s) 1817
BsuRI GGCC 6 cut(s) 61, 197, 248, 510, 974, 2052
BtgI CCRYGG 2 cut(s) 243, 1806
BtrI CACGTC 1 cut(s) 1945
BtsCI GGATG 7 cut(s) 66, 420, 931, 1360, 1441, 1591, 2261
BtsI GCAGTG 1 cut(s) 1863
BtsIMutI CAGTG 4 cut(s) 586, 896, 1611, 1863
BveI ACCTGC 1 cut(s) 1385
CciI TCATGA 1 cut(s) 1209
CfoI GCGC 2 cut(s) 210, 563
Cfr10I RCCGGY 1 cut(s) 2048
Cfr13I GGNCC 2 cut(s) 101, 2051
CseI GACGC 1 cut(s) 161
CsiI ACCWGGT 1 cut(s) 2170
Csp6I GTAC 7 cut(s) 272, 352, 551, 740, 1870, 1955, 2232
CspCI CAANNNNNGTGG 2 cut(s) 2155, 2190
CviAII CATG 8 cut(s) 244, 389, 512, 706, 1210, 1337, 1807, 2245
CviQI GTAC 7 cut(s) 272, 352, 551, 740, 1870, 1955, 2232
DraI TTTAAA 1 cut(s) 1048
DriI GACNNNNNGTC 1 cut(s) 2253
EaeI YGGCCR 2 cut(s) 246, 508
Eam1104I CTCTTC 2 cut(s) 1584, 2036
Eam1105I GACNNNNNGTC 1 cut(s) 2253
EarI CTCTTC 2 cut(s) 1584, 2036
EciI GGCGGA 1 cut(s) 1779
Ecl136II GAGCTC 1 cut(s) 542
Eco130I CCWWGG 2 cut(s) 243, 1806
Eco147I AGGCCT 2 cut(s) 61, 197
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 2 cut(s) 54, 2183
Eco32I GATATC 1 cut(s) 1786
Eco47I GGWCC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 854, 2118
Eco91I GGTNACC 1 cut(s) 2123
EcoICRI GAGCTC 1 cut(s) 542
EcoO65I GGTNACC 1 cut(s) 2123
EcoRI GAATTC 3 cut(s) 1466, 1664, 1829
EcoRII CCWGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
EcoRV GATATC 1 cut(s) 1786
EcoT14I CCWWGG 2 cut(s) 243, 1806
EcoT22I ATGCAT 1 cut(s) 1338
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 2 cut(s) 243, 1806
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 8 cut(s) 247, 392, 515, 709, 1213, 1340, 1810, 2248
FaqI GGGAC 2 cut(s) 488, 716
FatI CATG 8 cut(s) 243, 388, 511, 705, 1209, 1336, 1806, 2244
FauI CCCGC 1 cut(s) 1918
FblI GTMKAC 2 cut(s) 1321, 1941
Fnu4HI GCNGC 6 cut(s) 249, 456, 1167, 2013, 2325, 2346
FokI GGATG 7 cut(s) 53, 427, 938, 1367, 1428, 1598, 2248
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 6 cut(s) 249, 456, 1167, 2013, 2325, 2346
FspBI CTAG 7 cut(s) 950, 1014, 1328, 1895, 1958, 2016, 2283
GlaI GCGC 2 cut(s) 209, 562
GluI GCNGC 6 cut(s) 249, 456, 1167, 2013, 2325, 2346
GsaI CCCAGC 3 cut(s) 83, 591, 1194
GsuI CTGGAG 1 cut(s) 2119
HaeIII GGCC 6 cut(s) 61, 197, 248, 510, 974, 2052
HapII CCGG 1 cut(s) 2049
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 210, 563
Hin1II CATG 8 cut(s) 247, 392, 515, 709, 1213, 1340, 1810, 2248
Hin6I GCGC 2 cut(s) 208, 561
HinP1I GCGC 2 cut(s) 208, 561
HincII GTYRAC 3 cut(s) 661, 1942, 1984
HindII GTYRAC 3 cut(s) 661, 1942, 1984
HinfI GANTC 5 cut(s) 11, 166, 1248, 1985, 2192
HpaII CCGG 1 cut(s) 2049
HphI GGTGA 7 cut(s) 127, 514, 1092, 1388, 1430, 1634, 2135
Hpy166II GTNNAC 8 cut(s) 517, 661, 1322, 1702, 1747, 1942, 1984, 2234
Hpy188I TCNGA 9 cut(s) 20, 54, 291, 607, 865, 1602, 1796, 2030, 2313
Hpy8I GTNNAC 8 cut(s) 517, 661, 1322, 1702, 1747, 1942, 1984, 2234
Hpy99I CGWCG 2 cut(s) 1943, 1946
HpyAV CCTTC 5 cut(s) 386, 947, 1107, 1575, 1607
HpyCH4III ACNGT 8 cut(s) 32, 581, 613, 1346, 1411, 1606, 1709, 1916
HpyCH4IV ACGT 3 cut(s) 1281, 1459, 1944
HpyF10VI GCNNNNNNNGC 7 cut(s) 216, 248, 567, 1111, 1120, 1196, 2021
HpySE526I ACGT 3 cut(s) 1281, 1459, 1944
Hsp92II CATG 8 cut(s) 247, 392, 515, 709, 1213, 1340, 1810, 2248
HspAI GCGC 2 cut(s) 208, 561
KroI GCCGGC 1 cut(s) 2048
KroNI GCCGGC 1 cut(s) 2050
LmnI GCTCC 6 cut(s) 418, 535, 1532, 1688, 1810, 2021
Lsp1109I GCAGC 3 cut(s) 467, 1153, 2024
LweI GCATC 7 cut(s) 307, 1066, 1123, 1450, 1639, 2262, 2270
MabI ACCWGGT 1 cut(s) 2170
MaeI CTAG 7 cut(s) 950, 1014, 1328, 1895, 1958, 2016, 2283
MaeII ACGT 3 cut(s) 1281, 1459, 1944
MaeIII GTNAC 8 cut(s) 712, 894, 1411, 1430, 1910, 2089, 2123, 2162
MfeI CAATTG 1 cut(s) 1494
MflI RGATCY 1 cut(s) 1229
MhlI GDGCHC 4 cut(s) 544, 645, 1693, 1749
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 2 cut(s) 20, 1979
MmeI TCCRAC 3 cut(s) 207, 903, 1666
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 1338
MroNI GCCGGC 1 cut(s) 2048
MscI TGGCCA 1 cut(s) 510
MseI TTAA 8 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545, 2130
MslI CAYNNNNRTG 2 cut(s) 315, 710
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 1 cut(s) 2049
MspR9I CCNGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
MunI CAATTG 1 cut(s) 1494
Mva1269I GAATGC 3 cut(s) 1199, 1294, 2000
MvaI CCWGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
MwoI GCNNNNNNNGC 7 cut(s) 216, 248, 567, 1111, 1120, 1196, 2021
NaeI GCCGGC 1 cut(s) 2050
NcoI CCATGG 2 cut(s) 243, 1806
NgoMIV GCCGGC 1 cut(s) 2048
NlaIII CATG 8 cut(s) 247, 392, 515, 709, 1213, 1340, 1810, 2248
NlaIV GGNNCC 8 cut(s) 597, 642, 727, 760, 1003, 1231, 1812, 2202
NmuCI GTSAC 5 cut(s) 712, 894, 1430, 2123, 2162
NsiI ATGCAT 1 cut(s) 1338
NspI RCATGY 1 cut(s) 1340
PaeI GCATGC 1 cut(s) 1340
PagI TCATGA 1 cut(s) 1209
PceI AGGCCT 2 cut(s) 61, 197
PcsI WCGNNNNNNNCGW 1 cut(s) 1080
PctI GAATGC 3 cut(s) 1199, 1294, 2000
PdiI GCCGGC 1 cut(s) 2050
PfeI GAWTC 3 cut(s) 166, 1248, 2192
PflFI GACNNNGTC 1 cut(s) 1770
PflMI CCANNNNNTGG 1 cut(s) 1190
PfoI TCCNGGA 1 cut(s) 2135
PkrI GCNGC 6 cut(s) 250, 457, 1168, 2014, 2326, 2347
PleI GAGTC 2 cut(s) 19, 1979
PpsI GAGTC 2 cut(s) 19, 1979
Ppu21I YACGTR 1 cut(s) 1282
Psp124BI GAGCTC 1 cut(s) 544
Psp6I CCWGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
PspEI GGTNACC 1 cut(s) 2123
PspFI CCCAGC 3 cut(s) 79, 587, 1190
PspGI CCWGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
PspN4I GGNNCC 8 cut(s) 597, 642, 727, 760, 1003, 1231, 1812, 2202
PspPI GGNCC 2 cut(s) 101, 2051
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 1229
PsyI GACNNNGTC 1 cut(s) 1770
RsaI GTAC 7 cut(s) 273, 353, 552, 741, 1871, 1956, 2233
RsaNI GTAC 7 cut(s) 272, 352, 551, 740, 1870, 1955, 2232
RseI CAYNNNNRTG 2 cut(s) 315, 710
SacI GAGCTC 1 cut(s) 544
SalI GTCGAC 1 cut(s) 1940
SaqAI TTAA 8 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545, 2130
SatI GCNGC 6 cut(s) 249, 456, 1167, 2013, 2325, 2346
Sau96I GGNCC 2 cut(s) 101, 2051
SchI GAGTC 2 cut(s) 20, 1979
ScrFI CCNGG 7 cut(s) 507, 646, 1697, 1815, 1878, 2137, 2172
SduI GDGCHC 4 cut(s) 544, 645, 1693, 1749
SexAI ACCWGGT 1 cut(s) 2170
SfaNI GCATC 7 cut(s) 307, 1066, 1123, 1450, 1639, 2262, 2270
SfcI CTRYAG 2 cut(s) 6, 453
SgrDI CGTCGACG 1 cut(s) 1940
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 2 cut(s) 315, 710
SmlI CTYRAG 5 cut(s) 1579, 1634, 1655, 1987, 2180
SmoI CTYRAG 5 cut(s) 1579, 1634, 1655, 1987, 2180
SpeI ACTAGT 1 cut(s) 1957
SphI GCATGC 1 cut(s) 1340
SseBI AGGCCT 2 cut(s) 61, 197
SsiI CCGC 8 cut(s) 249, 694, 1103, 1764, 1925, 2210, 2325, 2346
SspI AATATT 1 cut(s) 295
SspMI CTAG 7 cut(s) 950, 1014, 1328, 1895, 1958, 2016, 2283
SstI GAGCTC 1 cut(s) 544
StuI AGGCCT 2 cut(s) 61, 197
StyD4I CCNGG 7 cut(s) 505, 644, 1695, 1813, 1876, 2135, 2170
StyI CCWWGG 2 cut(s) 243, 1806
TaaI ACNGT 8 cut(s) 32, 581, 613, 1346, 1411, 1606, 1709, 1916
TaiI ACGT 3 cut(s) 1284, 1462, 1947
TaqI TCGA 5 cut(s) 528, 1074, 1611, 1674, 1941
TatI WGTACW 5 cut(s) 351, 739, 1869, 1954, 2231
TauI GCSGC 3 cut(s) 251, 2327, 2348
TfiI GAWTC 3 cut(s) 166, 1248, 2192
Tru1I TTAA 8 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545, 2130
Tru9I TTAA 8 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545, 2130
TscAI CASTG 4 cut(s) 586, 903, 1611, 1863
TseFI GTSAC 5 cut(s) 712, 894, 1430, 2123, 2162
TseI GCWGC 3 cut(s) 455, 1166, 2012
Tsp45I GTSAC 5 cut(s) 712, 894, 1430, 2123, 2162
TspDTI ATGAA 8 cut(s) 405, 945, 1194, 1557, 1602, 1901, 2054, 2090
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 4 cut(s) 586, 903, 1611, 1863
Tth111I GACNNNGTC 1 cut(s) 1770
Van91I CCANNNNNTGG 1 cut(s) 1190
VneI GTGCAC 1 cut(s) 1745
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 5 cut(s) 718, 747, 1466, 1664, 1829
XbaI TCTAGA 2 cut(s) 949, 1327
XceI RCATGY 1 cut(s) 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmiI GTMKAC 2 cut(s) 1321, 1941
XspI CTAG 7 cut(s) 950, 1014, 1328, 1895, 1958, 2016, 2283
Zsp2I ATGCAT 1 cut(s) 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.