FvH4_5g00390

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010128
Physical Location & Seq
Reverse (-)
275093 .. 278909
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00390.t3

Sequence Viewer

Length: 2259 bp
ATGGCTGCAGAGTCAGTGAGAGCAGTTCAGAGTTTCAGCTTCAATGGGTCTCAGACAAGGCCAACCATCCCTCTCTCCCCACCTCGCTTTCTGGGTCTTCGTCCTCCTCGCTCTTCTTCTTCTTCTCTCACTTCCTCTTCCCTTTCCCACTTCTTCGGGAATCTCCGTCTCGCCTCCAACTCCTCAAAGACCTCTACTTTGGGCCGCAGAAACCTCTCTGTTCTCGCCATGGCCGCCGATGATGGAAAGCGTGCAGTACCGTTAGAAGATTATCGGAATATTGGAATTATGGCTCACATAGATGCAGGGAAGACTACTACAACTGAACGGGTTTTGTACTATACGGGAAGAAATTATAAAATAGGGGAGGTGCATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCTGCAACTACAACCTTTTGGGACAAACACAGAATTAACATTATTGATACTCCTGGCCATGTGGACTTCACCCTTGAGGTGGAGCGAGCTCTCAGGGTGCTTGATGGCGCAATATGCTTGTTTGACAGTGTTGCTGGGGTGGAACCTCAGTCTGAAACTGTATGGAGGCAAGCTGATAAATATGGGGTGCCCAGGATTTGCTTTGTCAACAAAATGGATCGACTTGGGGCAAATTTTTTCCGCACAAGGGACATGATAGTGTCGAATTTGGGTGCCAAACCACTTGTACTTCAAATTCCAGTTGGTGCTGAAGATAATTTTAAGGGAGTTATTGATCTTGTGAAGATGAGAGCTATAATTTGGTCAGGAGAAGAATTGGGTGCAAAATTTACGTATGAGGATATTCCCTCTGATCTTCAGGAGCTGGCTGATGAATACAGATCACTGATGATAGAAACCATAGTTGAGTTGGATGATGAAGCTATGGAGGGGTATCTAGAAGGGGTTGAACCTGATGAGGCCGCCATTAAGAAATTGATTAGGCAGGGAACCATCTCAGCTAGTTTTGTGCCAGTATTATGTGGCTCAGCTTTTAAAAATAAGGGGGTTCAACCATTGCTTGATGCTGTTGTGGATTATTTACCCTCACCACTTGATGTGCCACCAATGCAGGGAACTGATGCAGATAACCCAGAAATCACAATTGAGAGGGCTGCAAGTGATGACGAACCATTTGCTGGGCTGGCATTCAAGATCATGAGCGATCCATTTGTGGGATCCCTTACATTTGTGAGAGTCTATGCAGGAAAGCTAAGTGCTGGATCATATGTGCTGAATGCAAACAAGGGAAAAAAGGAGAGGATTGGAAGACTTCTAGAGATGCATGCAAACAGTAGGGAGGATGTTAAGGTCGCTTTAACTGGTGATATTATTGCGCTTGCAGGTCTAAAAGATACTGTTACTGGTGAAACATTGAGTGACCCTGAGCATCCTATTGTGCTTGAACGTATGGAATTCCCTGATCCTGTGATTAAGGTGGCAATTGAACCCAAAACTAAAGCTGACGTTGATAAGATGGGTGCTGGTTTGGTTAAGCTTGCTCAGGAAGACCCTTCGTTCCACTTCTCAAGAGATGAAGAGGTCGAGGCTAATGTTGGAGCACCCCAGGTTAACTACCGTGAAAGCATTTCTAAAGTCGCTGAAGTAAAGTATGTGCACAAGAAACAATCTGGTGGACAGGGTCAATTCGCAGATATCACTGTCCGATTTGAACCCATGGAGCCCGGTAATGGGTATGAGTTCAAGAGTGAAATCAAGGGAGGTGCAGTGCCCAGAGAGTACATTCCTGGGGTGATGAAAGGACTAGAGGAGTGCATGAGTAACGGTGTGCTTGCAGGCTTTCCTGTCGTCGACGTGCGTGCTGTACTAACCGATGGTTCTTACCACGATGTTGACTCGAGTGTTTTGGCATTCCAGTTGGCAGCGAGAGGAGCTTTCCGAGAGGGTATGAAGAGAGCTGGCCCAAAAATGCTTGAACCCATAATGAAAGTTGAAGTAGTTACACCTGAAGAACATTTGGGAGATGTGATTGGTGACCTTAACTCCAGGAGAGGTCAGATCAACAGCTTTAGCGACAAACCTGGTGGTCTCAAGGTGGTCGATGCTGAGGTTCCTCTTGCGGAGATGTTCCAATACGTTAGTACACTAAGGGGCATGACAAAAGGTCGTGCATCCTACACGATGCAATTAGCTAAGTTTGATGTTGTTCCTCAGCACATTCAGAACCAACTTTCTGCCAAGGAGGAAGAAGTTACTGCTTGA

Protein Analysis

753

Amino Acids

82.37

Weight (kDa)

5.18

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 90 - 362 4.6e-67 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 390 - 472 7.6e-16 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 405 - 471 1.4e-15 Elongation factor Tu domain 2
EFG_III PF14492 485 - 558 7e-32 Elongation Factor G, domain III
RF3_C PF16658 495 - 573 2.1e-06 Class II release factor RF3, C-terminal domain
EFG_IV PF03764 560 - 678 7.8e-46 Elongation factor G, domain IV
EFG_C PF00679 682 - 768 3.3e-27 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 1370
AccB1I GGYRCC 2 cut(s) 625, 710
AccB7I CCANNNNNTGG 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 1848
AciI CCGC 5 cut(s) 205, 234, 679, 960, 2117
AclWI GGATC 6 cut(s) 663, 1196, 1209, 1222, 1267, 1454
AcoI YGGCCR 2 cut(s) 231, 493
AcsI RAATTY 5 cut(s) 670, 703, 732, 824, 1451
AcuI CTGAAG 4 cut(s) 768, 839, 1659, 2025
AfaI GTAC 6 cut(s) 258, 338, 726, 1778, 1863, 2140
AfiI CCNNNNNNNGG 7 cut(s) 517, 685, 1109, 1175, 1211, 1727, 1728
AhdI GACNNNNNGTC 1 cut(s) 2160
AjiI CACGTC 1 cut(s) 1852
AjnI CCWGG 6 cut(s) 490, 629, 1602, 1783, 2042, 2077
Alw21I GWGCWC 3 cut(s) 529, 1600, 1656
Alw26I GTCTC 3 cut(s) 54, 173, 2090
Alw44I GTGCAC 1 cut(s) 1652
AlwI GGATC 6 cut(s) 663, 1196, 1209, 1222, 1267, 1454
AlwNI CAGNNNCTG 1 cut(s) 862
Ama87I CYCGRG 1 cut(s) 1894
AoxI GGCC 6 cut(s) 59, 202, 231, 493, 957, 1957
ApaLI GTGCAC 1 cut(s) 1652
ApeKI GCWGC 4 cut(s) 5, 440, 1151, 1919
ApoI RAATTY 5 cut(s) 670, 703, 732, 824, 1451
ArsI GACNNNNNNTTYG 4 cut(s) 181, 213, 1154, 1186
AspLEI GCGC 2 cut(s) 548, 1375
AspS9I GGNCC 2 cut(s) 202, 1958
AsuC2I CCSGG 1 cut(s) 1722
AsuHPI GGTGA 6 cut(s) 499, 1077, 1373, 1415, 1801, 2042
AvaI CYCGRG 1 cut(s) 1894
BaeGI GKGCMC 3 cut(s) 630, 1656, 1770
BalI TGGCCA 1 cut(s) 495
BamHI GGATCC 1 cut(s) 1214
BanI GGYRCC 2 cut(s) 625, 710
BanII GRGCYC 2 cut(s) 529, 1722
BbsI GAAGAC 4 cut(s) 89, 317, 1312, 1551
Bbv12I GWGCWC 3 cut(s) 529, 1600, 1656
BbvCI CCTCAGC 2 cut(s) 2103, 2208
BbvI GCAGC 3 cut(s) 427, 1138, 1931
BccI CCATC 7 cut(s) 74, 236, 394, 536, 998, 1507, 1865
BcgI CGANNNNNNTGC 4 cut(s) 1154, 1188, 1838, 1872
BciT130I CCWGG 6 cut(s) 492, 631, 1604, 1785, 2044, 2079
BcnI CCSGG 1 cut(s) 1722
BcoDI GTCTC 3 cut(s) 54, 173, 2090
BfaI CTAG 4 cut(s) 935, 999, 1313, 1802
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 1370
BisI GCNGC 7 cut(s) 6, 205, 234, 441, 960, 1152, 1920
BlpI GCTNAGC 1 cut(s) 1024
BlsI GCNGC 7 cut(s) 7, 206, 235, 442, 961, 1153, 1921
Bme1390I CCNGG 7 cut(s) 492, 631, 1604, 1722, 1785, 2044, 2079
BmeRI GACNNNNNGTC 1 cut(s) 2160
BmeT110I CYCGRG 1 cut(s) 1894
BmgBI CACGTC 1 cut(s) 1852
BmgT120I GGNCC 2 cut(s) 202, 1958
BmiI GGNNCC 7 cut(s) 582, 627, 712, 988, 1216, 1719, 2109
BmrFI CCNGG 7 cut(s) 492, 631, 1604, 1722, 1785, 2044, 2079
BmsI GCATC 8 cut(s) 292, 1051, 1108, 1308, 1435, 2089, 2169, 2177
BpiI GAAGAC 4 cut(s) 89, 317, 1312, 1551
BpmI CTGGAG 1 cut(s) 2026
Bpu10I CCTNAGC 4 cut(s) 1422, 1539, 2103, 2208
Bpu1102I GCTNAGC 1 cut(s) 1024
BpuEI CTTGAG 3 cut(s) 533, 1549, 2072
BpuMI CCSGG 1 cut(s) 1722
BsaAI YACGTR 1 cut(s) 831
BsaI GGTCTC 2 cut(s) 54, 2090
BsaJI CCNNGG 6 cut(s) 228, 629, 1602, 1713, 1784, 2235
BsaXI ACNNNNNCTCC 4 cut(s) 1749, 1779, 2024, 2054
Bsc4I CCNNNNNNNGG 7 cut(s) 517, 685, 1109, 1175, 1211, 1727, 1728
Bse1I ACTGG 6 cut(s) 401, 737, 1010, 1363, 1405, 1912
Bse3DI GCAATG 1 cut(s) 1052
BseBI CCWGG 6 cut(s) 492, 631, 1604, 1785, 2044, 2079
BseDI CCNNGG 6 cut(s) 228, 629, 1602, 1713, 1784, 2235
BseGI GGATG 6 cut(s) 66, 405, 916, 1345, 1426, 2168
BseLI CCNNNNNNNGG 7 cut(s) 517, 685, 1109, 1175, 1211, 1727, 1728
BseMI GCAATG 1 cut(s) 1052
BseMII CTCAG 9 cut(s) 65, 544, 599, 1008, 1038, 1413, 1553, 2094, 2222
BseNI ACTGG 6 cut(s) 401, 737, 1010, 1363, 1405, 1912
BseRI GAGGAG 4 cut(s) 96, 172, 1820, 1941
BseSI GKGCMC 3 cut(s) 630, 1656, 1770
BseXI GCAGC 3 cut(s) 427, 1138, 1931
BseYI CCCAGC 2 cut(s) 572, 1175
BsgI GTGCAG 2 cut(s) 273, 1782
BshFI GGCC 6 cut(s) 61, 204, 233, 495, 959, 1959
BshNI GGYRCC 2 cut(s) 625, 710
BsiHKAI GWGCWC 3 cut(s) 529, 1600, 1656
BsiHKCI CYCGRG 1 cut(s) 1894
BsiSI CCGG 1 cut(s) 1722
BslFI GGGAC 2 cut(s) 473, 701
BslI CCNNNNNNNGG 7 cut(s) 517, 685, 1109, 1175, 1211, 1727, 1728
BsmAI GTCTC 3 cut(s) 54, 173, 2090
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 473, 701
BsmI GAATGC 3 cut(s) 1184, 1279, 1907
BsnI GGCC 6 cut(s) 61, 204, 233, 495, 959, 1959
Bso31I GGTCTC 2 cut(s) 54, 2090
BsoBI CYCGRG 1 cut(s) 1894
Bsp1286I GDGCHC 6 cut(s) 529, 630, 1600, 1656, 1722, 1770
Bsp1720I GCTNAGC 1 cut(s) 1024
Bsp19I CCATGG 2 cut(s) 228, 1713
BspACI CCGC 5 cut(s) 205, 234, 679, 960, 2117
BspANI GGCC 6 cut(s) 61, 204, 233, 495, 959, 1959
BspCNI CTCAG 9 cut(s) 64, 543, 598, 1007, 1037, 1414, 1552, 2095, 2221
BspHI TCATGA 1 cut(s) 1194
BspLI GGNNCC 7 cut(s) 582, 627, 712, 988, 1216, 1719, 2109
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 1370
BspPI GGATC 6 cut(s) 663, 1196, 1209, 1222, 1267, 1454
BspQI GCTCTTC 1 cut(s) 118
BspT107I GGYRCC 2 cut(s) 625, 710
BspTNI GGTCTC 2 cut(s) 54, 2090
BsrDI GCAATG 1 cut(s) 1052
BsrI ACTGG 6 cut(s) 401, 737, 1010, 1363, 1405, 1912
BssECI CCNNGG 6 cut(s) 228, 629, 1602, 1713, 1784, 2235
BssT1I CCWWGG 3 cut(s) 228, 1713, 2235
Bst2UI CCWGG 6 cut(s) 492, 631, 1604, 1785, 2044, 2079
Bst4CI ACNGT 8 cut(s) 261, 566, 598, 1331, 1396, 1616, 1699, 1823
Bst6I CTCTTC 4 cut(s) 118, 142, 1569, 1943
BstBAI YACGTR 1 cut(s) 831
BstDSI CCRYGG 2 cut(s) 228, 1713
BstEII GGTNACC 1 cut(s) 2030
BstF5I GGATG 6 cut(s) 66, 405, 916, 1345, 1426, 2168
BstHHI GCGC 2 cut(s) 548, 1375
BstMAI GTCTC 3 cut(s) 54, 173, 2090
BstMWI GCNNNNNNNGC 5 cut(s) 233, 552, 1105, 1181, 1928
BstNI CCWGG 6 cut(s) 492, 631, 1604, 1785, 2044, 2079
BstNSI RCATGY 1 cut(s) 1325
BstPI GGTNACC 1 cut(s) 2030
BstSCI CCNGG 7 cut(s) 490, 629, 1602, 1720, 1783, 2042, 2077
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 3 cut(s) 630, 1656, 1770
BstSNI TACGTA 1 cut(s) 831
BstV1I GCAGC 3 cut(s) 427, 1138, 1931
BstV2I GAAGAC 4 cut(s) 89, 317, 1312, 1551
BstX2I RGATCY 1 cut(s) 1214
BstYI RGATCY 1 cut(s) 1214
BsuRI GGCC 6 cut(s) 61, 204, 233, 495, 959, 1959
BtgI CCRYGG 2 cut(s) 228, 1713
BtrI CACGTC 1 cut(s) 1852
BtsCI GGATG 6 cut(s) 66, 405, 916, 1345, 1426, 2168
BtsI GCAGTG 1 cut(s) 1770
BtsIMutI CAGTG 5 cut(s) 21, 571, 881, 1695, 1770
BveI ACCTGC 1 cut(s) 1370
CaiI CAGNNNCTG 1 cut(s) 862
CciI TCATGA 1 cut(s) 1194
CfoI GCGC 2 cut(s) 548, 1375
Cfr13I GGNCC 2 cut(s) 202, 1958
CsiI ACCWGGT 1 cut(s) 2077
Csp6I GTAC 6 cut(s) 257, 337, 725, 1777, 1862, 2139
CspCI CAANNNNNGTGG 2 cut(s) 2062, 2097
CviAII CATG 9 cut(s) 229, 374, 497, 691, 1195, 1322, 1714, 1813, 2152
CviQI GTAC 6 cut(s) 257, 337, 725, 1777, 1862, 2139
DraI TTTAAA 1 cut(s) 1033
DriI GACNNNNNGTC 1 cut(s) 2160
EaeI YGGCCR 2 cut(s) 231, 493
Eam1104I CTCTTC 4 cut(s) 118, 142, 1569, 1943
Eam1105I GACNNNNNGTC 1 cut(s) 2160
EarI CTCTTC 4 cut(s) 118, 142, 1569, 1943
Ecl136II GAGCTC 1 cut(s) 527
Eco105I TACGTA 1 cut(s) 831
Eco130I CCWWGG 3 cut(s) 228, 1713, 2235
Eco24I GRGCYC 2 cut(s) 529, 1722
Eco31I GGTCTC 2 cut(s) 54, 2090
Eco32I GATATC 1 cut(s) 1693
Eco53kI GAGCTC 1 cut(s) 527
Eco57I CTGAAG 4 cut(s) 768, 839, 1659, 2025
Eco88I CYCGRG 1 cut(s) 1894
Eco91I GGTNACC 1 cut(s) 2030
EcoICRI GAGCTC 1 cut(s) 527
EcoO65I GGTNACC 1 cut(s) 2030
EcoRI GAATTC 1 cut(s) 1451
EcoRII CCWGG 6 cut(s) 490, 629, 1602, 1783, 2042, 2077
EcoRV GATATC 1 cut(s) 1693
EcoT14I CCWWGG 3 cut(s) 228, 1713, 2235
EcoT22I ATGCAT 1 cut(s) 1323
EcoT38I GRGCYC 2 cut(s) 529, 1722
ErhI CCWWGG 3 cut(s) 228, 1713, 2235
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 9 cut(s) 232, 377, 500, 694, 1198, 1325, 1717, 1816, 2155
FaqI GGGAC 2 cut(s) 473, 701
FatI CATG 9 cut(s) 228, 373, 496, 690, 1194, 1321, 1713, 1812, 2151
FauNDI CATATG 1 cut(s) 1264
FblI GTMKAC 1 cut(s) 1848
Fnu4HI GCNGC 7 cut(s) 6, 205, 234, 441, 960, 1152, 1920
FokI GGATG 6 cut(s) 53, 412, 923, 1352, 1413, 2155
FriOI GRGCYC 2 cut(s) 529, 1722
Fsp4HI GCNGC 7 cut(s) 6, 205, 234, 441, 960, 1152, 1920
FspBI CTAG 4 cut(s) 935, 999, 1313, 1802
GlaI GCGC 2 cut(s) 547, 1374
GluI GCNGC 7 cut(s) 6, 205, 234, 441, 960, 1152, 1920
GsaI CCCAGC 2 cut(s) 576, 1179
GsuI CTGGAG 1 cut(s) 2026
HaeIII GGCC 6 cut(s) 61, 204, 233, 495, 959, 1959
HapII CCGG 1 cut(s) 1722
HhaI GCGC 2 cut(s) 548, 1375
Hin1II CATG 9 cut(s) 232, 377, 500, 694, 1198, 1325, 1717, 1816, 2155
Hin6I GCGC 2 cut(s) 546, 1373
HinP1I GCGC 2 cut(s) 546, 1373
HincII GTYRAC 4 cut(s) 646, 1609, 1849, 1891
HindII GTYRAC 4 cut(s) 646, 1609, 1849, 1891
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 4 cut(s) 11, 160, 1233, 1892
HpaI GTTAAC 1 cut(s) 1609
HpaII CCGG 1 cut(s) 1722
HphI GGTGA 6 cut(s) 499, 1077, 1373, 1415, 1801, 2042
Hpy166II GTNNAC 8 cut(s) 502, 646, 1609, 1654, 1673, 1849, 1891, 2141
Hpy188I TCNGA 9 cut(s) 30, 54, 276, 592, 850, 1703, 1937, 2055, 2220
Hpy8I GTNNAC 8 cut(s) 502, 646, 1609, 1654, 1673, 1849, 1891, 2141
Hpy99I CGWCG 2 cut(s) 1850, 1853
HpyAV CCTTC 3 cut(s) 371, 932, 1560
HpyCH4III ACNGT 8 cut(s) 261, 566, 598, 1331, 1396, 1616, 1699, 1823
HpyCH4IV ACGT 5 cut(s) 830, 1444, 1503, 1851, 2133
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 552, 1105, 1181, 1928
HpySE526I ACGT 5 cut(s) 830, 1444, 1503, 1851, 2133
Hsp92II CATG 9 cut(s) 232, 377, 500, 694, 1198, 1325, 1717, 1816, 2155
HspAI GCGC 2 cut(s) 546, 1373
KspAI GTTAAC 1 cut(s) 1609
LguI GCTCTTC 1 cut(s) 118
LmnI GCTCC 6 cut(s) 403, 520, 859, 1595, 1717, 1928
Lsp1109I GCAGC 3 cut(s) 427, 1138, 1931
LweI GCATC 8 cut(s) 292, 1051, 1108, 1308, 1435, 2089, 2169, 2177
MabI ACCWGGT 1 cut(s) 2077
MaeI CTAG 4 cut(s) 935, 999, 1313, 1802
MaeII ACGT 5 cut(s) 830, 1444, 1503, 1851, 2133
MaeIII GTNAC 6 cut(s) 1396, 1415, 1817, 1996, 2030, 2248
MfeI CAATTG 2 cut(s) 1140, 1479
MflI RGATCY 1 cut(s) 1214
MhlI GDGCHC 6 cut(s) 529, 630, 1600, 1656, 1722, 1770
MlsI TGGCCA 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 3 cut(s) 20, 1242, 1886
MmeI TCCRAC 3 cut(s) 201, 888, 1573
Mox20I TGGCCA 1 cut(s) 495
Mph1103I ATGCAT 1 cut(s) 1323
MscI TGGCCA 1 cut(s) 495
MslI CAYNNNNRTG 2 cut(s) 300, 695
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 1 cut(s) 1722
MspR9I CCNGG 7 cut(s) 492, 631, 1604, 1722, 1785, 2044, 2079
MunI CAATTG 2 cut(s) 1140, 1479
Mva1269I GAATGC 3 cut(s) 1184, 1279, 1907
MvaI CCWGG 6 cut(s) 492, 631, 1604, 1785, 2044, 2079
MwoI GCNNNNNNNGC 5 cut(s) 233, 552, 1105, 1181, 1928
NciI CCSGG 1 cut(s) 1722
NcoI CCATGG 2 cut(s) 228, 1713
NdeI CATATG 1 cut(s) 1264
NlaIII CATG 9 cut(s) 232, 377, 500, 694, 1198, 1325, 1717, 1816, 2155
NlaIV GGNNCC 7 cut(s) 582, 627, 712, 988, 1216, 1719, 2109
NmuCI GTSAC 2 cut(s) 1415, 2030
NsiI ATGCAT 1 cut(s) 1323
NspI RCATGY 1 cut(s) 1325
PaeI GCATGC 1 cut(s) 1325
PaeR7I CTCGAG 1 cut(s) 1894
PagI TCATGA 1 cut(s) 1194
PciSI GCTCTTC 1 cut(s) 118
PctI GAATGC 3 cut(s) 1184, 1279, 1907
PfeI GAWTC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 1677
PflMI CCANNNNNTGG 1 cut(s) 1175
PfoI TCCNGGA 1 cut(s) 2042
PkrI GCNGC 7 cut(s) 7, 206, 235, 442, 961, 1153, 1921
PleI GAGTC 3 cut(s) 19, 1241, 1886
PpsI GAGTC 3 cut(s) 19, 1241, 1886
Ppu21I YACGTR 1 cut(s) 831
PsiI TTATAA 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 529
Psp6I CCWGG 6 cut(s) 490, 629, 1602, 1783, 2042, 2077
PspEI GGTNACC 1 cut(s) 2030
PspFI CCCAGC 2 cut(s) 572, 1175
PspGI CCWGG 6 cut(s) 490, 629, 1602, 1783, 2042, 2077
PspN4I GGNNCC 7 cut(s) 582, 627, 712, 988, 1216, 1719, 2109
PspPI GGNCC 2 cut(s) 202, 1958
PspXI VCTCGAGB 1 cut(s) 1894
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 862
PsuI RGATCY 1 cut(s) 1214
PsyI GACNNNGTC 1 cut(s) 1677
RsaI GTAC 6 cut(s) 258, 338, 726, 1778, 1863, 2140
RsaNI GTAC 6 cut(s) 257, 337, 725, 1777, 1862, 2139
RseI CAYNNNNRTG 2 cut(s) 300, 695
SacI GAGCTC 1 cut(s) 529
SalI GTCGAC 1 cut(s) 1847
SapI GCTCTTC 1 cut(s) 118
SatI GCNGC 7 cut(s) 6, 205, 234, 441, 960, 1152, 1920
Sau96I GGNCC 2 cut(s) 202, 1958
SchI GAGTC 3 cut(s) 20, 1242, 1886
ScrFI CCNGG 7 cut(s) 492, 631, 1604, 1722, 1785, 2044, 2079
SduI GDGCHC 6 cut(s) 529, 630, 1600, 1656, 1722, 1770
SexAI ACCWGGT 1 cut(s) 2077
SfaNI GCATC 8 cut(s) 292, 1051, 1108, 1308, 1435, 2089, 2169, 2177
SfcI CTRYAG 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 1894
SgrDI CGTCGACG 1 cut(s) 1847
SlaI CTCGAG 1 cut(s) 1894
SmiMI CAYNNNNRTG 2 cut(s) 300, 695
SmlI CTYRAG 4 cut(s) 512, 1564, 1894, 2087
SmoI CTYRAG 4 cut(s) 512, 1564, 1894, 2087
SnaBI TACGTA 1 cut(s) 831
SphI GCATGC 1 cut(s) 1325
SsiI CCGC 5 cut(s) 205, 234, 679, 960, 2117
SspI AATATT 1 cut(s) 280
SspMI CTAG 4 cut(s) 935, 999, 1313, 1802
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 7 cut(s) 490, 629, 1602, 1720, 1783, 2042, 2077
StyI CCWWGG 3 cut(s) 228, 1713, 2235
TaaI ACNGT 8 cut(s) 261, 566, 598, 1331, 1396, 1616, 1699, 1823
TaiI ACGT 5 cut(s) 833, 1447, 1506, 1854, 2136
TaqI TCGA 6 cut(s) 658, 701, 1581, 1848, 1895, 2097
TatI WGTACW 5 cut(s) 336, 724, 1776, 1861, 2138
TauI GCSGC 3 cut(s) 207, 236, 962
TfiI GAWTC 1 cut(s) 160
TscAI CASTG 5 cut(s) 21, 571, 888, 1702, 1770
TseFI GTSAC 2 cut(s) 1415, 2030
TseI GCWGC 4 cut(s) 5, 440, 1151, 1919
Tsp45I GTSAC 2 cut(s) 1415, 2030
TspDTI ATGAA 7 cut(s) 390, 885, 930, 1587, 1808, 1961, 1997
TspGWI ACGGA 1 cut(s) 155
TspRI CASTG 5 cut(s) 21, 571, 888, 1702, 1770
Tth111I GACNNNGTC 1 cut(s) 1677
Van91I CCANNNNNTGG 1 cut(s) 1175
VneI GTGCAC 1 cut(s) 1652
XapI RAATTY 5 cut(s) 670, 703, 732, 824, 1451
XbaI TCTAGA 2 cut(s) 934, 1312
XceI RCATGY 1 cut(s) 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XhoI CTCGAG 1 cut(s) 1894
XmiI GTMKAC 1 cut(s) 1848
XspI CTAG 4 cut(s) 935, 999, 1313, 1802
Zsp2I ATGCAT 1 cut(s) 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.