Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010128

Physical Location & Seq

Genomic Coordinates Forward (+)

13524250 .. 13525760

Gene Structure 1511 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1065300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 750 bp

ATGGGAGCTGGTTTGGTTAAGCTTGCTCAAGAAGACCCCTCTTTCCACTTCTCACGGGATGAAGAGATAAACCAGACAGTGATTGAAGGAATGGGAGAATTGCATCTTGAAATTATTGTTGATCGGCTCAAGAGAGAATTTAAGGTTGAAGCGAATGTTGGAGCACCCCAGGTTAACTACCAGGAAAGCATTTCCAGGACCTCAGAAGTGAGGTATGTGCACAAGAAACAATCAGGTGGACAAGGTCAGTTTGCAGATATCACCGTACGATTTGAACCAATGGAGCCTGTGCCAAGAGAATACATTCCCGGGGTGATGAAAGGATTAGAGGAGTGTATGAGTAACGGTGTACTTGCAGGCTTTCCTGTCGTCGACGCACGCGCTGTACTAGTAGATGGTTCTTACCAAGATGTCGACTCAAGCGTGTTGGCATTTCAATTGGCAGCAAGAGGGGCTTTCCGTGATGGAATGAAGAAAGCTGCCCCGAAAATGCTTGATCCAATAATGAAAGTTGAAGTTGTTACACCTGAAGAACATCTTGGAGACGTTATTGGTGATCTCAACTCAAGGAGAGGACAGATCAACAGCTTTAATGACAAGCCTGGTGGTCTCAAGGTGGTGGATGCACTGGTTCCTCTTGCAGAGATGTTTCAGTACGTTAGTACGCTACGGGGCATGACAAAAGGTCGTGCATCGTATACCATGCAGTTAGCTAAGTTTGATGTTGTTCCTCAGCACATTCAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009845 GO:0009941 GO:0009987 GO:0016043 GO:0017076 GO:0030554 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0071840 GO:0090351 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

250

Amino Acids

27.5

Weight (kDa)

5.47

Isoelectric Point (pI)

38.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFG_III	PF14492	1 - 54	3e-21	Elongation Factor G, domain III
RF3_C	PF16658	3 - 70	2.3e-06	Class II release factor RF3, C-terminal domain
EFG_IV	PF03764	56 - 160	3e-31	Elongation factor G, domain IV
EFG_C	PF00679	164 - 248	2.5e-25	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62750
fragaria_vesca	FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 FvH4_5g00390
malus_domestica	MD04G1081800.v1.1 MD06G1065300.v1.1
prunus_persica	Prupe.5G076600_v2.0.a1 Prupe.5G076600_v2.0.a1
pyrus_communis	pycom04g07370 pycom06g05630
rosa_chinensis	RchiOBHm_Chr7g0202121
rosa_laevigata	RLG00000003624
rosa_multiflora	Rmu_ssc0000228.1_g000002
rosa_roxburghii	Rroxscaffold_3G00254840
rosa_rugosa	Rorug07G0068100
rosa_samantha	Rh7AG195600 Rh7BG196900 Rh7CG206700 Rh7DG202200
rosa_wichuraiana	Rw7G017130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	372, 414, 698
AccII	CGCG	1 cut(s)	381
AclWI	GGATC	1 cut(s)	491
AcsI	RAATTY	1 cut(s)	137
AcuI	CTGAAG	1 cut(s)	549
AfaI	GTAC	5 cut(s)	267, 351, 387, 656, 664
AgsI	TTSAA	6 cut(s)	86, 110, 149, 275, 437, 515
AhdI	GACNNNNNGTC	1 cut(s)	684
AhlI	ACTAGT	1 cut(s)	388
AjnI	CCWGG	4 cut(s)	168, 180, 194, 601
AjuI	GAANNNNNNNTTGG	4 cut(s)	141, 173, 522, 554
AluBI	AGCT	5 cut(s)	8, 22, 479, 588, 713
AluI	AGCT	5 cut(s)	8, 22, 479, 588, 713
Alw21I	GWGCWC	2 cut(s)	166, 222
Alw26I	GTCTC	2 cut(s)	537, 614
Alw44I	GTGCAC	1 cut(s)	218
AlwI	GGATC	1 cut(s)	491
Ama87I	CYCGRG	1 cut(s)	308
ApaLI	GTGCAC	1 cut(s)	218
ApeKI	GCWGC	2 cut(s)	443, 479
ApoI	RAATTY	1 cut(s)	137
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	1 cut(s)	383
AspS9I	GGNCC	1 cut(s)	198
AsuC2I	CCSGG	2 cut(s)	309, 310
AsuHPI	GGTGA	3 cut(s)	253, 325, 566
AvaI	CYCGRG	1 cut(s)	308
AvaII	GGWCC	1 cut(s)	198
BaeGI	GKGCMC	1 cut(s)	222
BbsI	GAAGAC	1 cut(s)	39
Bbv12I	GWGCWC	2 cut(s)	166, 222
BbvCI	CCTCAGC	1 cut(s)	732
BbvI	GCAGC	2 cut(s)	455, 466
BccI	CCATC	2 cut(s)	389, 458
BciT130I	CCWGG	4 cut(s)	170, 182, 196, 603
BcnI	CCSGG	2 cut(s)	309, 310
BcoDI	GTCTC	2 cut(s)	537, 614
BcuI	ACTAGT	1 cut(s)	388
BfaI	CTAG	2 cut(s)	389, 748
BisI	GCNGC	2 cut(s)	444, 480
BlsI	GCNGC	2 cut(s)	445, 481
Bme1390I	CCNGG	6 cut(s)	170, 182, 196, 309, 310, 603
Bme18I	GGWCC	1 cut(s)	198
BmeRI	GACNNNNNGTC	1 cut(s)	684
BmeT110I	CYCGRG	1 cut(s)	308
BmgT120I	GGNCC	1 cut(s)	198
BmiI	GGNNCC	2 cut(s)	285, 633
BmrFI	CCNGG	6 cut(s)	170, 182, 196, 309, 310, 603
BmsI	GCATC	3 cut(s)	112, 613, 701
BpiI	GAAGAC	1 cut(s)	39
Bpu10I	CCTNAGC	1 cut(s)	732
BpuEI	CTTGAG	5 cut(s)	12, 113, 403, 550, 596
BpuMI	CCSGG	2 cut(s)	309, 310
BsaI	GGTCTC	1 cut(s)	614
BsaJI	CCNNGG	3 cut(s)	168, 308, 309
Bse1I	ACTGG	1 cut(s)	633
BseBI	CCWGG	4 cut(s)	170, 182, 196, 603
BseDI	CCNNGG	3 cut(s)	168, 308, 309
BseGI	GGATG	2 cut(s)	64, 628
BseMII	CTCAG	2 cut(s)	216, 746
BseNI	ACTGG	1 cut(s)	633
BseRI	GAGGAG	1 cut(s)	344
BseSI	GKGCMC	1 cut(s)	222
BseXI	GCAGC	2 cut(s)	455, 466
Bsh1236I	CGCG	1 cut(s)	381
BsiHKAI	GWGCWC	2 cut(s)	166, 222
BsiHKCI	CYCGRG	1 cut(s)	308
BsiSI	CCGG	1 cut(s)	309
BsiWI	CGTACG	1 cut(s)	265
BsmAI	GTCTC	2 cut(s)	537, 614
BsmBI	CGTCTC	1 cut(s)	537
Bso31I	GGTCTC	1 cut(s)	614
BsoBI	CYCGRG	1 cut(s)	308
Bsp1286I	GDGCHC	2 cut(s)	166, 222
Bsp143I	GATC	4 cut(s)	121, 496, 556, 579
BspCNI	CTCAG	2 cut(s)	215, 745
BspFNI	CGCG	1 cut(s)	381
BspLI	GGNNCC	2 cut(s)	285, 633
BspPI	GGATC	1 cut(s)	491
BspTNI	GGTCTC	1 cut(s)	614
BsrI	ACTGG	1 cut(s)	633
BssECI	CCNNGG	3 cut(s)	168, 308, 309
BssMI	GATC	4 cut(s)	121, 496, 556, 579
BssNAI	GTATAC	1 cut(s)	699
Bst1107I	GTATAC	1 cut(s)	699
Bst2UI	CCWGG	4 cut(s)	170, 182, 196, 603
Bst4CI	ACNGT	3 cut(s)	79, 265, 347
Bst6I	CTCTTC	1 cut(s)	57
BstC8I	GCNNGC	3 cut(s)	24, 358, 379
BstDEI	CTNAG	3 cut(s)	202, 714, 732
BstF5I	GGATG	2 cut(s)	64, 628
BstFNI	CGCG	1 cut(s)	381
BstHHI	GCGC	1 cut(s)	383
BstKTI	GATC	4 cut(s)	124, 499, 559, 582
BstMAI	GTCTC	2 cut(s)	537, 614
BstMBI	GATC	4 cut(s)	121, 496, 556, 579
BstMWI	GCNNNNNNNGC	1 cut(s)	452
BstNI	CCWGG	4 cut(s)	170, 182, 196, 603
BstSCI	CCNGG	6 cut(s)	168, 180, 194, 307, 308, 601
BstSLI	GKGCMC	1 cut(s)	222
BstUI	CGCG	1 cut(s)	381
BstV1I	GCAGC	2 cut(s)	455, 466
BstV2I	GAAGAC	1 cut(s)	39
BstZ17I	GTATAC	1 cut(s)	699
BtsCI	GGATG	2 cut(s)	64, 628
BtsIMutI	CAGTG	2 cut(s)	84, 626
Cac8I	GCNNGC	3 cut(s)	24, 358, 379
CfoI	GCGC	1 cut(s)	383
Cfr13I	GGNCC	1 cut(s)	198
Cfr9I	CCCGGG	1 cut(s)	308
CseI	GACGC	1 cut(s)	383
Csp6I	GTAC	5 cut(s)	266, 350, 386, 655, 663
CspCI	CAANNNNNGTGG	2 cut(s)	586, 621
CviAII	CATG	2 cut(s)	676, 703
CviQI	GTAC	5 cut(s)	266, 350, 386, 655, 663
DdeI	CTNAG	3 cut(s)	202, 714, 732
DpnI	GATC	4 cut(s)	123, 498, 558, 581
DpnII	GATC	4 cut(s)	121, 496, 556, 579
DriI	GACNNNNNGTC	1 cut(s)	684
Eam1104I	CTCTTC	1 cut(s)	57
Eam1105I	GACNNNNNGTC	1 cut(s)	684
EarI	CTCTTC	1 cut(s)	57
Eco31I	GGTCTC	1 cut(s)	614
Eco32I	GATATC	1 cut(s)	259
Eco47I	GGWCC	1 cut(s)	198
Eco57I	CTGAAG	1 cut(s)	549
Eco88I	CYCGRG	1 cut(s)	308
EcoO109I	RGGNCCY	1 cut(s)	198
EcoRII	CCWGG	4 cut(s)	168, 180, 194, 601
EcoRV	GATATC	1 cut(s)	259
Esp3I	CGTCTC	1 cut(s)	537
FaeI	CATG	2 cut(s)	679, 706
FaiI	YATR	5 cut(s)	216, 338, 677, 699, 704
FalI	AAGNNNNNCTT	4 cut(s)	439, 471, 522, 554
FatI	CATG	2 cut(s)	675, 702
FblI	GTMKAC	3 cut(s)	372, 414, 698
Fnu4HI	GCNGC	2 cut(s)	444, 480
FokI	GGATG	2 cut(s)	71, 635
Fsp4HI	GCNGC	2 cut(s)	444, 480
FspBI	CTAG	2 cut(s)	389, 748
GlaI	GCGC	1 cut(s)	382
GluI	GCNGC	2 cut(s)	444, 480
HapII	CCGG	1 cut(s)	309
HgaI	GACGC	1 cut(s)	383
HhaI	GCGC	1 cut(s)	383
Hin1II	CATG	2 cut(s)	679, 706
Hin6I	GCGC	1 cut(s)	381
HinP1I	GCGC	1 cut(s)	381
HincII	GTYRAC	3 cut(s)	175, 373, 415
HindII	GTYRAC	3 cut(s)	175, 373, 415
HindIII	AAGCTT	1 cut(s)	20
HinfI	GANTC	1 cut(s)	416
HpaI	GTTAAC	1 cut(s)	175
HpaII	CCGG	1 cut(s)	309
HphI	GGTGA	3 cut(s)	253, 325, 566
Hpy166II	GTNNAC	7 cut(s)	175, 220, 239, 350, 373, 415, 699
Hpy188I	TCNGA	2 cut(s)	205, 744
Hpy188III	TCNNGA	3 cut(s)	29, 107, 130
Hpy8I	GTNNAC	7 cut(s)	175, 220, 239, 350, 373, 415, 699
Hpy99I	CGWCG	2 cut(s)	374, 377
HpyAV	CCTTC	1 cut(s)	80
HpyCH4III	ACNGT	3 cut(s)	79, 265, 347
HpyCH4IV	ACGT	2 cut(s)	546, 657
HpyCH4V	TGCA	8 cut(s)	103, 220, 254, 356, 626, 641, 692, 706
HpyF10VI	GCNNNNNNNGC	1 cut(s)	452
HpyF3I	CTNAG	3 cut(s)	202, 714, 732
HpySE526I	ACGT	2 cut(s)	546, 657
Hsp92II	CATG	2 cut(s)	679, 706
HspAI	GCGC	1 cut(s)	381
KspAI	GTTAAC	1 cut(s)	175
Kzo9I	GATC	4 cut(s)	121, 496, 556, 579
LmnI	GCTCC	3 cut(s)	5, 161, 283
Lsp1109I	GCAGC	2 cut(s)	455, 466
LweI	GCATC	3 cut(s)	112, 613, 701
MaeI	CTAG	2 cut(s)	389, 748
MaeII	ACGT	2 cut(s)	546, 657
MaeIII	GTNAC	2 cut(s)	341, 520
MalI	GATC	4 cut(s)	123, 498, 558, 581
MboI	GATC	4 cut(s)	121, 496, 556, 579
MboII	GAAGA	4 cut(s)	44, 74, 484, 542
MfeI	CAATTG	1 cut(s)	437
MhlI	GDGCHC	2 cut(s)	166, 222
MluCI	AATT	4 cut(s)	98, 111, 137, 437
MlyI	GAGTC	1 cut(s)	410
MmeI	TCCRAC	1 cut(s)	139
MnlI	CCTC	8 cut(s)	49, 204, 211, 322, 443, 566, 645, 741
MroXI	GAANNNNTTC	1 cut(s)	303
MseI	TTAA	4 cut(s)	18, 141, 174, 591
MspI	CCGG	1 cut(s)	309
MspR9I	CCNGG	6 cut(s)	170, 182, 196, 309, 310, 603
MunI	CAATTG	1 cut(s)	437
MvaI	CCWGG	4 cut(s)	170, 182, 196, 603
MvnI	CGCG	1 cut(s)	381
MwoI	GCNNNNNNNGC	1 cut(s)	452
NciI	CCSGG	2 cut(s)	309, 310
NdeII	GATC	4 cut(s)	121, 496, 556, 579
NlaIII	CATG	2 cut(s)	679, 706
NlaIV	GGNNCC	2 cut(s)	285, 633
PcsI	WCGNNNNNNNCGW	1 cut(s)	420
PdmI	GAANNNNTTC	1 cut(s)	303
Pfl23II	CGTACG	1 cut(s)	265
PflFI	GACNNNGTC	1 cut(s)	243
PfoI	TCCNGGA	1 cut(s)	194
PkrI	GCNGC	2 cut(s)	445, 481
PleI	GAGTC	1 cut(s)	410
PpsI	GAGTC	1 cut(s)	410
PpuMI	RGGWCCY	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	198
Psp6I	CCWGG	4 cut(s)	168, 180, 194, 601
PspGI	CCWGG	4 cut(s)	168, 180, 194, 601
PspLI	CGTACG	1 cut(s)	265
PspN4I	GGNNCC	2 cut(s)	285, 633
PspPI	GGNCC	1 cut(s)	198
PspPPI	RGGWCCY	1 cut(s)	198
PsyI	GACNNNGTC	1 cut(s)	243
RsaI	GTAC	5 cut(s)	267, 351, 387, 656, 664
RsaNI	GTAC	5 cut(s)	266, 350, 386, 655, 663
SalI	GTCGAC	2 cut(s)	371, 413
SaqAI	TTAA	4 cut(s)	18, 141, 174, 591
SatI	GCNGC	2 cut(s)	444, 480
Sau3AI	GATC	4 cut(s)	121, 496, 556, 579
Sau96I	GGNCC	1 cut(s)	198
SchI	GAGTC	1 cut(s)	410
ScrFI	CCNGG	6 cut(s)	170, 182, 196, 309, 310, 603
SduI	GDGCHC	2 cut(s)	166, 222
SfaNI	GCATC	3 cut(s)	112, 613, 701
SgrDI	CGTCGACG	1 cut(s)	371
SinI	GGWCC	1 cut(s)	198
SmaI	CCCGGG	1 cut(s)	310
SmlI	CTYRAG	5 cut(s)	27, 128, 418, 565, 611
SmoI	CTYRAG	5 cut(s)	27, 128, 418, 565, 611
SpeI	ACTAGT	1 cut(s)	388
Sse9I	AATT	4 cut(s)	98, 111, 137, 437
SspMI	CTAG	2 cut(s)	389, 748
StyD4I	CCNGG	6 cut(s)	168, 180, 194, 307, 308, 601
TaaI	ACNGT	3 cut(s)	79, 265, 347
TaiI	ACGT	2 cut(s)	549, 660
TaqI	TCGA	2 cut(s)	372, 414
TasI	AATT	4 cut(s)	98, 111, 137, 437
TatI	WGTACW	2 cut(s)	349, 385
Tru1I	TTAA	4 cut(s)	18, 141, 174, 591
Tru9I	TTAA	4 cut(s)	18, 141, 174, 591
TscAI	CASTG	2 cut(s)	84, 633
TseI	GCWGC	2 cut(s)	443, 479
TspDTI	ATGAA	4 cut(s)	75, 332, 485, 521
TspGWI	ACGGA	1 cut(s)	449
TspMI	CCCGGG	1 cut(s)	308
TspRI	CASTG	2 cut(s)	84, 633
Tth111I	GACNNNGTC	1 cut(s)	243
VneI	GTGCAC	1 cut(s)	218
VpaK11BI	GGWCC	1 cut(s)	198
XapI	RAATTY	1 cut(s)	137
XmaI	CCCGGG	1 cut(s)	308
XmiI	GTMKAC	3 cut(s)	372, 414, 698
XmnI	GAANNNNTTC	1 cut(s)	303
XspI	CTAG	2 cut(s)	389, 748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.