Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G62750 |
| fragaria_vesca | FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 |
| malus_domestica | MD04G1081800.v1.1 MD06G1065300.v1.1 |
| prunus_persica | Prupe.5G076600_v2.0.a1 Prupe.5G076600_v2.0.a1 |
| pyrus_communis | pycom04g07370 pycom06g05630 |
| rosa_chinensis | RchiOBHm_Chr7g0202121 |
| rosa_laevigata | RLG00000003624 |
| rosa_multiflora | Rmu_ssc0000228.1_g000002 |
| rosa_roxburghii | Rroxscaffold_3G00254840 |
| rosa_rugosa | Rorug07G0068100 |
| rosa_samantha | Rh7AG195600 Rh7BG196900 Rh7CG206700 Rh7DG202200 |
| rosa_wichuraiana | Rw7G017130 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 628, 713 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1178 |
| AccI | GTMKAC | 2 cut(s) | 1309, 1971 |
| AccII | CGCG | 2 cut(s) | 308, 1938 |
| AciI | CCGC | 3 cut(s) | 157, 308, 2227 |
| AclWI | GGATC | 7 cut(s) | 666, 1199, 1212, 1225, 1257, 1270, 1457 |
| AcoI | YGGCCR | 1 cut(s) | 496 |
| AcsI | RAATTY | 4 cut(s) | 636, 735, 827, 1652 |
| AfaI | GTAC | 9 cut(s) | 26, 540, 729, 1050, 1272, 1730, 1935, 1944, 2221 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 520, 1112, 1178, 1214, 1648, 1808 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 2241 |
| AhlI | ACTAGT | 1 cut(s) | 1945 |
| AjiI | CACGTC | 1 cut(s) | 1099 |
| AjnI | CCWGG | 7 cut(s) | 493, 1683, 1709, 1801, 1864, 2123, 2158 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1656, 1688 |
| Alw21I | GWGCWC | 4 cut(s) | 378, 532, 1681, 1737 |
| Alw26I | GTCTC | 4 cut(s) | 51, 601, 684, 2171 |
| Alw44I | GTGCAC | 2 cut(s) | 374, 1733 |
| AlwI | GGATC | 7 cut(s) | 666, 1199, 1212, 1225, 1257, 1270, 1457 |
| AoxI | GGCC | 3 cut(s) | 56, 496, 1384 |
| ApaLI | GTGCAC | 2 cut(s) | 374, 1733 |
| ApeKI | GCWGC | 8 cut(s) | 236, 443, 1154, 1256, 2000, 2036, 2312, 2333 |
| ApoI | RAATTY | 4 cut(s) | 636, 735, 827, 1652 |
| AseI | ATTAAT | 1 cut(s) | 977 |
| AspLEI | GCGC | 3 cut(s) | 1378, 1940, 2185 |
| AsuC2I | CCSGG | 1 cut(s) | 2018 |
| AsuHPI | GGTGA | 7 cut(s) | 112, 502, 1080, 1376, 1418, 1882, 2123 |
| AsuNHI | GCTAGC | 2 cut(s) | 1183, 1252 |
| AxyI | CCTNAGG | 1 cut(s) | 2085 |
| BaeGI | GKGCMC | 3 cut(s) | 378, 633, 1737 |
| BalI | TGGCCA | 1 cut(s) | 498 |
| BamHI | GGATCC | 2 cut(s) | 1217, 1262 |
| BanI | GGYRCC | 2 cut(s) | 628, 713 |
| BanII | GRGCYC | 1 cut(s) | 532 |
| BauI | CACGAG | 1 cut(s) | 779 |
| BbsI | GAAGAC | 3 cut(s) | 86, 320, 1554 |
| Bbv12I | GWGCWC | 4 cut(s) | 378, 532, 1681, 1737 |
| BbvCI | CCTCAGC | 1 cut(s) | 2289 |
| BbvI | GCAGC | 7 cut(s) | 223, 430, 1141, 1243, 2012, 2023, 2299 |
| BccI | CCATC | 8 cut(s) | 239, 397, 539, 856, 1001, 1510, 1600, 1946 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1714, 1748 |
| BciT130I | CCWGG | 7 cut(s) | 495, 1685, 1711, 1803, 1866, 2125, 2160 |
| BciVI | GTATCC | 1 cut(s) | 1805 |
| BcnI | CCSGG | 1 cut(s) | 2018 |
| BcoDI | GTCTC | 4 cut(s) | 51, 601, 684, 2171 |
| BcuI | ACTAGT | 1 cut(s) | 1945 |
| BfaI | CTAG | 5 cut(s) | 938, 1184, 1253, 1316, 1946 |
| BfmI | CTRYAG | 1 cut(s) | 6 |
| BfuI | GTATCC | 1 cut(s) | 1805 |
| BisI | GCNGC | 9 cut(s) | 158, 237, 444, 1155, 1257, 2001, 2037, 2313, 2334 |
| BlpI | GCTNAGC | 2 cut(s) | 161, 1027 |
| BlsI | GCNGC | 9 cut(s) | 159, 238, 445, 1156, 1258, 2002, 2038, 2314, 2335 |
| BmcAI | AGTACT | 1 cut(s) | 540 |
| Bme1390I | CCNGG | 8 cut(s) | 495, 1685, 1711, 1803, 1866, 2018, 2125, 2160 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 2241 |
| BmgBI | CACGTC | 1 cut(s) | 1099 |
| BmiI | GGNNCC | 8 cut(s) | 585, 630, 715, 991, 1219, 1264, 1800, 2190 |
| BmrFI | CCNGG | 8 cut(s) | 495, 1685, 1711, 1803, 1866, 2018, 2125, 2160 |
| BmsI | GCATC | 6 cut(s) | 1054, 1111, 1438, 1627, 2170, 2258 |
| BmtI | GCTAGC | 2 cut(s) | 1187, 1256 |
| BpiI | GAAGAC | 3 cut(s) | 86, 320, 1554 |
| BpmI | CTGGAG | 3 cut(s) | 828, 881, 2107 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1425, 2289 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 161, 1027 |
| BpuEI | CTTGAG | 5 cut(s) | 855, 1527, 1643, 1960, 2153 |
| BpuMI | CCSGG | 1 cut(s) | 2018 |
| BsaAI | YACGTR | 1 cut(s) | 1732 |
| BsaI | GGTCTC | 2 cut(s) | 51, 2171 |
| BsaJI | CCNNGG | 5 cut(s) | 1683, 1710, 1794, 1865, 2316 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1830, 1860 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 520, 1112, 1178, 1214, 1648, 1808 |
| Bse1I | ACTGG | 5 cut(s) | 352, 404, 546, 740, 1013 |
| Bse21I | CCTNAGG | 1 cut(s) | 2085 |
| BseBI | CCWGG | 7 cut(s) | 495, 1685, 1711, 1803, 1866, 2125, 2160 |
| BseDI | CCNNGG | 5 cut(s) | 1683, 1710, 1794, 1865, 2316 |
| BseGI | GGATG | 7 cut(s) | 408, 550, 1348, 1429, 1579, 1611, 2249 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 520, 1112, 1178, 1214, 1648, 1808 |
| BseMII | CTCAG | 7 cut(s) | 62, 175, 547, 1041, 1416, 2076, 2303 |
| BseNI | ACTGG | 5 cut(s) | 352, 404, 546, 740, 1013 |
| BseRI | GAGGAG | 3 cut(s) | 166, 1901, 2022 |
| BseSI | GKGCMC | 3 cut(s) | 378, 633, 1737 |
| BseXI | GCAGC | 7 cut(s) | 223, 430, 1141, 1243, 2012, 2023, 2299 |
| BseYI | CCCAGC | 1 cut(s) | 1178 |
| BsgI | GTGCAG | 2 cut(s) | 276, 768 |
| Bsh1236I | CGCG | 2 cut(s) | 308, 1938 |
| BshFI | GGCC | 3 cut(s) | 58, 498, 1386 |
| BshNI | GGYRCC | 2 cut(s) | 628, 713 |
| BsiHKAI | GWGCWC | 4 cut(s) | 378, 532, 1681, 1737 |
| BsiSI | CCGG | 1 cut(s) | 2017 |
| BslFI | GGGAC | 3 cut(s) | 84, 476, 1129 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 520, 1112, 1178, 1214, 1648, 1808 |
| BsmAI | GTCTC | 4 cut(s) | 51, 601, 684, 2171 |
| BsmFI | GGGAC | 3 cut(s) | 84, 476, 1129 |
| BsmI | GAATGC | 3 cut(s) | 1187, 1282, 1988 |
| BsnI | GGCC | 3 cut(s) | 58, 498, 1386 |
| Bso31I | GGTCTC | 2 cut(s) | 51, 2171 |
| Bsp1286I | GDGCHC | 5 cut(s) | 378, 532, 633, 1681, 1737 |
| Bsp1407I | TGTACA | 1 cut(s) | 24 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 161, 1027 |
| Bsp19I | CCATGG | 1 cut(s) | 1794 |
| BspACI | CCGC | 3 cut(s) | 157, 308, 2227 |
| BspANI | GGCC | 3 cut(s) | 58, 498, 1386 |
| BspCNI | CTCAG | 7 cut(s) | 61, 174, 546, 1040, 1417, 2077, 2302 |
| BspFNI | CGCG | 2 cut(s) | 308, 1938 |
| BspHI | TCATGA | 1 cut(s) | 1197 |
| BspLI | GGNNCC | 8 cut(s) | 585, 630, 715, 991, 1219, 1264, 1800, 2190 |
| BspOI | GCTAGC | 2 cut(s) | 1187, 1256 |
| BspPI | GGATC | 7 cut(s) | 666, 1199, 1212, 1225, 1257, 1270, 1457 |
| BspT107I | GGYRCC | 2 cut(s) | 628, 713 |
| BspTNI | GGTCTC | 2 cut(s) | 51, 2171 |
| BsrGI | TGTACA | 1 cut(s) | 24 |
| BsrI | ACTGG | 5 cut(s) | 352, 404, 546, 740, 1013 |
| BssECI | CCNNGG | 5 cut(s) | 1683, 1710, 1794, 1865, 2316 |
| BssSI | CACGAG | 1 cut(s) | 779 |
| BssT1I | CCWWGG | 2 cut(s) | 1794, 2316 |
| Bst2BI | CACGAG | 1 cut(s) | 779 |
| Bst2UI | CCWGG | 7 cut(s) | 495, 1685, 1711, 1803, 1866, 2125, 2160 |
| Bst4CI | ACNGT | 7 cut(s) | 29, 569, 601, 1334, 1594, 1780, 1904 |
| Bst6I | CTCTTC | 3 cut(s) | 115, 807, 1572 |
| BstAUI | TGTACA | 1 cut(s) | 24 |
| BstBAI | YACGTR | 1 cut(s) | 1732 |
| BstDEI | CTNAG | 8 cut(s) | 48, 161, 533, 1027, 1425, 2085, 2271, 2289 |
| BstDSI | CCRYGG | 1 cut(s) | 1794 |
| BstF5I | GGATG | 7 cut(s) | 408, 550, 1348, 1429, 1579, 1611, 2249 |
| BstFNI | CGCG | 2 cut(s) | 308, 1938 |
| BstHHI | GCGC | 3 cut(s) | 1378, 1940, 2185 |
| BstMAI | GTCTC | 4 cut(s) | 51, 601, 684, 2171 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 204, 236, 259, 1184, 2009 |
| BstNI | CCWGG | 7 cut(s) | 495, 1685, 1711, 1803, 1866, 2125, 2160 |
| BstSCI | CCNGG | 8 cut(s) | 493, 1683, 1709, 1801, 1864, 2016, 2123, 2158 |
| BstSFI | CTRYAG | 1 cut(s) | 6 |
| BstSLI | GKGCMC | 3 cut(s) | 378, 633, 1737 |
| BstUI | CGCG | 2 cut(s) | 308, 1938 |
| BstV1I | GCAGC | 7 cut(s) | 223, 430, 1141, 1243, 2012, 2023, 2299 |
| BstV2I | GAAGAC | 3 cut(s) | 86, 320, 1554 |
| BstX2I | RGATCY | 2 cut(s) | 1217, 1262 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 146 |
| BstYI | RGATCY | 2 cut(s) | 1217, 1262 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2085 |
| BsuI | GTATCC | 1 cut(s) | 1805 |
| BsuRI | GGCC | 3 cut(s) | 58, 498, 1386 |
| BtgI | CCRYGG | 1 cut(s) | 1794 |
| BtrI | CACGTC | 1 cut(s) | 1099 |
| BtsCI | GGATG | 7 cut(s) | 408, 550, 1348, 1429, 1579, 1611, 2249 |
| BtsI | GCAGTG | 2 cut(s) | 264, 2222 |
| BtsIMutI | CAGTG | 6 cut(s) | 21, 264, 574, 1020, 1776, 2222 |
| CciI | TCATGA | 1 cut(s) | 1197 |
| CfoI | GCGC | 3 cut(s) | 1378, 1940, 2185 |
| CseI | GACGC | 1 cut(s) | 314 |
| CsiI | ACCWGGT | 1 cut(s) | 2158 |
| Csp6I | GTAC | 9 cut(s) | 25, 539, 728, 1049, 1271, 1729, 1934, 1943, 2220 |
| CviAII | CATG | 7 cut(s) | 500, 694, 1198, 1795, 1964, 2233, 2260 |
| CviQI | GTAC | 9 cut(s) | 25, 539, 728, 1049, 1271, 1729, 1934, 1943, 2220 |
| DdeI | CTNAG | 8 cut(s) | 48, 161, 533, 1027, 1425, 2085, 2271, 2289 |
| DraI | TTTAAA | 1 cut(s) | 1036 |
| DriI | GACNNNNNGTC | 1 cut(s) | 2241 |
| EaeI | YGGCCR | 1 cut(s) | 496 |
| Eam1104I | CTCTTC | 3 cut(s) | 115, 807, 1572 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 2241 |
| EarI | CTCTTC | 3 cut(s) | 115, 807, 1572 |
| Ecl136II | GAGCTC | 1 cut(s) | 530 |
| Eco130I | CCWWGG | 2 cut(s) | 1794, 2316 |
| Eco147I | AGGCCT | 1 cut(s) | 1386 |
| Eco24I | GRGCYC | 1 cut(s) | 532 |
| Eco31I | GGTCTC | 2 cut(s) | 51, 2171 |
| Eco32I | GATATC | 1 cut(s) | 1774 |
| Eco53kI | GAGCTC | 1 cut(s) | 530 |
| Eco81I | CCTNAGG | 1 cut(s) | 2085 |
| EcoICRI | GAGCTC | 1 cut(s) | 530 |
| EcoRI | GAATTC | 1 cut(s) | 1652 |
| EcoRII | CCWGG | 7 cut(s) | 493, 1683, 1709, 1801, 1864, 2123, 2158 |
| EcoRV | GATATC | 1 cut(s) | 1774 |
| EcoT14I | CCWWGG | 2 cut(s) | 1794, 2316 |
| EcoT38I | GRGCYC | 1 cut(s) | 532 |
| ErhI | CCWWGG | 2 cut(s) | 1794, 2316 |
| FaeI | CATG | 7 cut(s) | 503, 697, 1201, 1798, 1967, 2236, 2263 |
| FaqI | GGGAC | 3 cut(s) | 84, 476, 1129 |
| FatI | CATG | 7 cut(s) | 499, 693, 1197, 1794, 1963, 2232, 2259 |
| FauI | CCCGC | 2 cut(s) | 301, 2220 |
| FblI | GTMKAC | 2 cut(s) | 1309, 1971 |
| Fnu4HI | GCNGC | 9 cut(s) | 158, 237, 444, 1155, 1257, 2001, 2037, 2313, 2334 |
| FokI | GGATG | 7 cut(s) | 415, 557, 1355, 1416, 1586, 1618, 2236 |
| FriOI | GRGCYC | 1 cut(s) | 532 |
| Fsp4HI | GCNGC | 9 cut(s) | 158, 237, 444, 1155, 1257, 2001, 2037, 2313, 2334 |
| FspBI | CTAG | 5 cut(s) | 938, 1184, 1253, 1316, 1946 |
| GlaI | GCGC | 3 cut(s) | 1377, 1939, 2184 |
| GluI | GCNGC | 9 cut(s) | 158, 237, 444, 1155, 1257, 2001, 2037, 2313, 2334 |
| GsaI | CCCAGC | 1 cut(s) | 1182 |
| GsuI | CTGGAG | 3 cut(s) | 828, 881, 2107 |
| HaeIII | GGCC | 3 cut(s) | 58, 498, 1386 |
| HapII | CCGG | 1 cut(s) | 2017 |
| HgaI | GACGC | 1 cut(s) | 314 |
| HhaI | GCGC | 3 cut(s) | 1378, 1940, 2185 |
| Hin1II | CATG | 7 cut(s) | 503, 697, 1201, 1798, 1967, 2236, 2263 |
| Hin6I | GCGC | 3 cut(s) | 1376, 1938, 2183 |
| HinP1I | GCGC | 3 cut(s) | 1376, 1938, 2183 |
| HincII | GTYRAC | 2 cut(s) | 505, 1972 |
| HindII | GTYRAC | 2 cut(s) | 505, 1972 |
| HindIII | AAGCTT | 1 cut(s) | 1535 |
| HinfI | GANTC | 3 cut(s) | 11, 1236, 1973 |
| HpaII | CCGG | 1 cut(s) | 2017 |
| HphI | GGTGA | 7 cut(s) | 112, 502, 1080, 1376, 1418, 1882, 2123 |
| Hpy166II | GTNNAC | 9 cut(s) | 25, 376, 505, 835, 1310, 1735, 1754, 1972, 2222 |
| Hpy188I | TCNGA | 8 cut(s) | 51, 536, 595, 683, 853, 1590, 2136, 2301 |
| Hpy8I | GTNNAC | 9 cut(s) | 25, 376, 505, 835, 1310, 1735, 1754, 1972, 2222 |
| HpyAV | CCTTC | 7 cut(s) | 374, 935, 979, 1095, 1105, 1563, 1595 |
| HpyCH4III | ACNGT | 7 cut(s) | 29, 569, 601, 1334, 1594, 1780, 1904 |
| HpyCH4IV | ACGT | 2 cut(s) | 1098, 1731 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 204, 236, 259, 1184, 2009 |
| HpyF3I | CTNAG | 8 cut(s) | 48, 161, 533, 1027, 1425, 2085, 2271, 2289 |
| HpySE526I | ACGT | 2 cut(s) | 1098, 1731 |
| Hsp92II | CATG | 7 cut(s) | 503, 697, 1201, 1798, 1967, 2236, 2263 |
| HspAI | GCGC | 3 cut(s) | 1376, 1938, 2183 |
| LmnI | GCTCC | 8 cut(s) | 406, 523, 668, 862, 1520, 1676, 1798, 2009 |
| Lsp1109I | GCAGC | 7 cut(s) | 223, 430, 1141, 1243, 2012, 2023, 2299 |
| LweI | GCATC | 6 cut(s) | 1054, 1111, 1438, 1627, 2170, 2258 |
| MabI | ACCWGGT | 1 cut(s) | 2158 |
| MaeI | CTAG | 5 cut(s) | 938, 1184, 1253, 1316, 1946 |
| MaeII | ACGT | 2 cut(s) | 1098, 1731 |
| MaeIII | GTNAC | 4 cut(s) | 700, 882, 1418, 2077 |
| MfeI | CAATTG | 2 cut(s) | 1482, 1994 |
| MflI | RGATCY | 2 cut(s) | 1217, 1262 |
| MhlI | GDGCHC | 5 cut(s) | 378, 532, 633, 1681, 1737 |
| MlsI | TGGCCA | 1 cut(s) | 498 |
| MluNI | TGGCCA | 1 cut(s) | 498 |
| MlyI | GAGTC | 2 cut(s) | 20, 1967 |
| MmeI | TCCRAC | 2 cut(s) | 195, 1654 |
| Mox20I | TGGCCA | 1 cut(s) | 498 |
| MscI | TGGCCA | 1 cut(s) | 498 |
| MseI | TTAA | 8 cut(s) | 477, 762, 969, 977, 1035, 1347, 1473, 1533 |
| MslI | CAYNNNNRTG | 1 cut(s) | 698 |
| Msp20I | TGGCCA | 1 cut(s) | 498 |
| MspI | CCGG | 1 cut(s) | 2017 |
| MspR9I | CCNGG | 8 cut(s) | 495, 1685, 1711, 1803, 1866, 2018, 2125, 2160 |
| MunI | CAATTG | 2 cut(s) | 1482, 1994 |
| Mva1269I | GAATGC | 3 cut(s) | 1187, 1282, 1988 |
| MvaI | CCWGG | 7 cut(s) | 495, 1685, 1711, 1803, 1866, 2125, 2160 |
| MvnI | CGCG | 2 cut(s) | 308, 1938 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 204, 236, 259, 1184, 2009 |
| NciI | CCSGG | 1 cut(s) | 2018 |
| NcoI | CCATGG | 1 cut(s) | 1794 |
| NheI | GCTAGC | 2 cut(s) | 1183, 1252 |
| NlaIII | CATG | 7 cut(s) | 503, 697, 1201, 1798, 1967, 2236, 2263 |
| NlaIV | GGNNCC | 8 cut(s) | 585, 630, 715, 991, 1219, 1264, 1800, 2190 |
| NmuCI | GTSAC | 3 cut(s) | 700, 882, 1418 |
| PagI | TCATGA | 1 cut(s) | 1197 |
| PceI | AGGCCT | 1 cut(s) | 1386 |
| PctI | GAATGC | 3 cut(s) | 1187, 1282, 1988 |
| PfeI | GAWTC | 1 cut(s) | 1236 |
| PflFI | GACNNNGTC | 1 cut(s) | 1758 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1178 |
| PfoI | TCCNGGA | 2 cut(s) | 2016, 2123 |
| PkrI | GCNGC | 9 cut(s) | 159, 238, 445, 1156, 1258, 2002, 2038, 2314, 2335 |
| PleI | GAGTC | 2 cut(s) | 19, 1967 |
| PpsI | GAGTC | 2 cut(s) | 19, 1967 |
| Ppu21I | YACGTR | 1 cut(s) | 1732 |
| PshBI | ATTAAT | 1 cut(s) | 977 |
| Psp124BI | GAGCTC | 1 cut(s) | 532 |
| Psp6I | CCWGG | 7 cut(s) | 493, 1683, 1709, 1801, 1864, 2123, 2158 |
| PspFI | CCCAGC | 1 cut(s) | 1178 |
| PspGI | CCWGG | 7 cut(s) | 493, 1683, 1709, 1801, 1864, 2123, 2158 |
| PspN4I | GGNNCC | 8 cut(s) | 585, 630, 715, 991, 1219, 1264, 1800, 2190 |
| PsuI | RGATCY | 2 cut(s) | 1217, 1262 |
| PsyI | GACNNNGTC | 1 cut(s) | 1758 |
| RsaI | GTAC | 9 cut(s) | 26, 540, 729, 1050, 1272, 1730, 1935, 1944, 2221 |
| RsaNI | GTAC | 9 cut(s) | 25, 539, 728, 1049, 1271, 1729, 1934, 1943, 2220 |
| RseI | CAYNNNNRTG | 1 cut(s) | 698 |
| SacI | GAGCTC | 1 cut(s) | 532 |
| SalI | GTCGAC | 1 cut(s) | 1970 |
| SaqAI | TTAA | 8 cut(s) | 477, 762, 969, 977, 1035, 1347, 1473, 1533 |
| SatI | GCNGC | 9 cut(s) | 158, 237, 444, 1155, 1257, 2001, 2037, 2313, 2334 |
| ScaI | AGTACT | 1 cut(s) | 540 |
| SchI | GAGTC | 2 cut(s) | 20, 1967 |
| ScrFI | CCNGG | 8 cut(s) | 495, 1685, 1711, 1803, 1866, 2018, 2125, 2160 |
| SduI | GDGCHC | 5 cut(s) | 378, 532, 633, 1681, 1737 |
| SexAI | ACCWGGT | 1 cut(s) | 2158 |
| SfaNI | GCATC | 6 cut(s) | 1054, 1111, 1438, 1627, 2170, 2258 |
| SfcI | CTRYAG | 1 cut(s) | 6 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 698 |
| SmlI | CTYRAG | 5 cut(s) | 870, 1542, 1622, 1975, 2168 |
| SmoI | CTYRAG | 5 cut(s) | 870, 1542, 1622, 1975, 2168 |
| SpeI | ACTAGT | 1 cut(s) | 1945 |
| SseBI | AGGCCT | 1 cut(s) | 1386 |
| SsiI | CCGC | 3 cut(s) | 157, 308, 2227 |
| SspMI | CTAG | 5 cut(s) | 938, 1184, 1253, 1316, 1946 |
| SstI | GAGCTC | 1 cut(s) | 532 |
| StuI | AGGCCT | 1 cut(s) | 1386 |
| StyD4I | CCNGG | 8 cut(s) | 493, 1683, 1709, 1801, 1864, 2016, 2123, 2158 |
| StyI | CCWWGG | 2 cut(s) | 1794, 2316 |
| TaaI | ACNGT | 7 cut(s) | 29, 569, 601, 1334, 1594, 1780, 1904 |
| TaiI | ACGT | 2 cut(s) | 1101, 1734 |
| TaqI | TCGA | 3 cut(s) | 1971, 2070, 2277 |
| TatI | WGTACW | 6 cut(s) | 24, 538, 727, 1270, 1942, 2219 |
| TauI | GCSGC | 1 cut(s) | 160 |
| TfiI | GAWTC | 1 cut(s) | 1236 |
| Tru1I | TTAA | 8 cut(s) | 477, 762, 969, 977, 1035, 1347, 1473, 1533 |
| Tru9I | TTAA | 8 cut(s) | 477, 762, 969, 977, 1035, 1347, 1473, 1533 |
| TscAI | CASTG | 6 cut(s) | 21, 264, 574, 1020, 1783, 2229 |
| TseFI | GTSAC | 3 cut(s) | 700, 882, 1418 |
| TseI | GCWGC | 8 cut(s) | 236, 443, 1154, 1256, 2000, 2036, 2312, 2333 |
| Tsp45I | GTSAC | 3 cut(s) | 700, 882, 1418 |
| TspDTI | ATGAA | 7 cut(s) | 933, 972, 1124, 1182, 1590, 1889, 2078 |
| TspGWI | ACGGA | 1 cut(s) | 346 |
| TspRI | CASTG | 6 cut(s) | 21, 264, 574, 1020, 1783, 2229 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1758 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1178 |
| VneI | GTGCAC | 2 cut(s) | 374, 1733 |
| VspI | ATTAAT | 1 cut(s) | 977 |
| XapI | RAATTY | 4 cut(s) | 636, 735, 827, 1652 |
| XbaI | TCTAGA | 2 cut(s) | 937, 1315 |
| XmiI | GTMKAC | 2 cut(s) | 1309, 1971 |
| XspI | CTAG | 5 cut(s) | 938, 1184, 1253, 1316, 1946 |
| ZrmI | AGTACT | 1 cut(s) | 540 |