Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G62750 |
| fragaria_vesca | FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 FvH4_5g00390 |
| malus_domestica | MD04G1081800.v1.1 MD06G1065300.v1.1 |
| prunus_persica | Prupe.5G076600_v2.0.a1 Prupe.5G076600_v2.0.a1 |
| pyrus_communis | pycom04g07370 pycom06g05630 |
| rosa_chinensis | RchiOBHm_Chr7g0202121 |
| rosa_laevigata | RLG00000003624 |
| rosa_multiflora | Rmu_ssc0000228.1_g000002 |
| rosa_roxburghii | Rroxscaffold_3G00254840 |
| rosa_rugosa | Rorug07G0068100 |
| rosa_samantha | Rh7AG195600 Rh7BG196900 Rh7CG206700 Rh7DG202200 |
| rosa_wichuraiana | Rw7G017130 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 71 |
| AccI | GTMKAC | 2 cut(s) | 202, 1148 |
| AccII | CGCG | 1 cut(s) | 831 |
| AciI | CCGC | 2 cut(s) | 699, 1091 |
| AclWI | GGATC | 6 cut(s) | 92, 105, 118, 150, 163, 350 |
| AcsI | RAATTY | 1 cut(s) | 545 |
| AcuI | CTGAAG | 2 cut(s) | 302, 633 |
| AfaI | GTAC | 5 cut(s) | 675, 828, 837, 1106, 1114 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 71, 107 |
| AgeI | ACCGGT | 1 cut(s) | 296 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1134 |
| AhlI | ACTAGT | 1 cut(s) | 838 |
| AjnI | CCWGG | 3 cut(s) | 576, 757, 1051 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 549, 581 |
| Alw21I | GWGCWC | 3 cut(s) | 336, 574, 630 |
| Alw26I | GTCTC | 1 cut(s) | 1064 |
| Alw44I | GTGCAC | 2 cut(s) | 570, 626 |
| AlwI | GGATC | 6 cut(s) | 92, 105, 118, 150, 163, 350 |
| Ama87I | CYCGRG | 2 cut(s) | 462, 1015 |
| AoxI | GGCC | 1 cut(s) | 277 |
| ApaLI | GTGCAC | 2 cut(s) | 570, 626 |
| ApeKI | GCWGC | 5 cut(s) | 47, 893, 929, 1205, 1226 |
| ApoI | RAATTY | 1 cut(s) | 545 |
| AsiGI | ACCGGT | 1 cut(s) | 296 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 753 |
| AspLEI | GCGC | 1 cut(s) | 833 |
| AsuC2I | CCSGG | 2 cut(s) | 590, 911 |
| AsuHPI | GGTGA | 5 cut(s) | 269, 311, 661, 775, 1016 |
| AsuNHI | GCTAGC | 1 cut(s) | 76 |
| AvaI | CYCGRG | 2 cut(s) | 462, 1015 |
| BaeGI | GKGCMC | 2 cut(s) | 574, 630 |
| BamHI | GGATCC | 2 cut(s) | 110, 155 |
| BbsI | GAAGAC | 1 cut(s) | 447 |
| Bbv12I | GWGCWC | 3 cut(s) | 336, 574, 630 |
| BbvCI | CCTCAGC | 2 cut(s) | 1118, 1182 |
| BbvI | GCAGC | 5 cut(s) | 34, 905, 916, 1192, 1213 |
| BccI | CCATC | 2 cut(s) | 403, 839 |
| BciT130I | CCWGG | 3 cut(s) | 578, 759, 1053 |
| BciVI | GTATCC | 1 cut(s) | 280 |
| BcnI | CCSGG | 2 cut(s) | 590, 911 |
| BcoDI | GTCTC | 1 cut(s) | 1064 |
| BcuI | ACTAGT | 1 cut(s) | 838 |
| BfaI | CTAG | 3 cut(s) | 77, 209, 839 |
| BfuI | GTATCC | 1 cut(s) | 280 |
| BisI | GCNGC | 5 cut(s) | 48, 894, 930, 1206, 1227 |
| BlsI | GCNGC | 5 cut(s) | 49, 895, 931, 1207, 1228 |
| Bme1390I | CCNGG | 5 cut(s) | 578, 590, 759, 911, 1053 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1134 |
| BmeT110I | CYCGRG | 2 cut(s) | 462, 1015 |
| BmiI | GGNNCC | 4 cut(s) | 63, 112, 157, 1083 |
| BmrFI | CCNGG | 5 cut(s) | 578, 590, 759, 911, 1053 |
| BmsI | GCATC | 3 cut(s) | 4, 520, 1151 |
| BmtI | GCTAGC | 1 cut(s) | 80 |
| BpiI | GAAGAC | 1 cut(s) | 447 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1118, 1182 |
| BpuEI | CTTGAG | 5 cut(s) | 420, 521, 536, 853, 1046 |
| BpuMI | CCSGG | 2 cut(s) | 590, 911 |
| BsaAI | YACGTR | 1 cut(s) | 1108 |
| BsaI | GGTCTC | 1 cut(s) | 1064 |
| BsaJI | CCNNGG | 3 cut(s) | 576, 758, 1209 |
| BsaWI | WCCGGW | 1 cut(s) | 296 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 723, 753 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 71, 107 |
| Bse118I | RCCGGY | 1 cut(s) | 296 |
| Bse1I | ACTGG | 1 cut(s) | 1083 |
| BseBI | CCWGG | 3 cut(s) | 578, 759, 1053 |
| BseDI | CCNNGG | 3 cut(s) | 576, 758, 1209 |
| BseGI | GGATG | 3 cut(s) | 241, 322, 472 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 71, 107 |
| BseMII | CTCAG | 2 cut(s) | 1109, 1196 |
| BseNI | ACTGG | 1 cut(s) | 1083 |
| BseRI | GAGGAG | 3 cut(s) | 794, 915, 1033 |
| BseSI | GKGCMC | 2 cut(s) | 574, 630 |
| BseXI | GCAGC | 5 cut(s) | 34, 905, 916, 1192, 1213 |
| BseYI | CCCAGC | 1 cut(s) | 71 |
| BsgI | GTGCAG | 1 cut(s) | 756 |
| Bsh1236I | CGCG | 1 cut(s) | 831 |
| BshFI | GGCC | 1 cut(s) | 279 |
| BshTI | ACCGGT | 1 cut(s) | 296 |
| BsiHKAI | GWGCWC | 3 cut(s) | 336, 574, 630 |
| BsiHKCI | CYCGRG | 2 cut(s) | 462, 1015 |
| BsiSI | CCGG | 3 cut(s) | 297, 589, 910 |
| BsiWI | CGTACG | 2 cut(s) | 673, 826 |
| BslFI | GGGAC | 1 cut(s) | 22 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 71, 107 |
| BsmAI | GTCTC | 1 cut(s) | 1064 |
| BsmFI | GGGAC | 1 cut(s) | 22 |
| BsmI | GAATGC | 1 cut(s) | 80 |
| BsnI | GGCC | 1 cut(s) | 279 |
| Bso31I | GGTCTC | 1 cut(s) | 1064 |
| BsoBI | CYCGRG | 2 cut(s) | 462, 1015 |
| Bsp1286I | GDGCHC | 3 cut(s) | 336, 574, 630 |
| BspACI | CCGC | 2 cut(s) | 699, 1091 |
| BspANI | GGCC | 1 cut(s) | 279 |
| BspCNI | CTCAG | 2 cut(s) | 1110, 1195 |
| BspFNI | CGCG | 1 cut(s) | 831 |
| BspHI | TCATGA | 1 cut(s) | 90 |
| BspLI | GGNNCC | 4 cut(s) | 63, 112, 157, 1083 |
| BspOI | GCTAGC | 1 cut(s) | 80 |
| BspPI | GGATC | 6 cut(s) | 92, 105, 118, 150, 163, 350 |
| BspTNI | GGTCTC | 1 cut(s) | 1064 |
| BsrFI | RCCGGY | 1 cut(s) | 296 |
| BsrI | ACTGG | 1 cut(s) | 1083 |
| BssAI | RCCGGY | 1 cut(s) | 296 |
| BssECI | CCNNGG | 3 cut(s) | 576, 758, 1209 |
| BssNAI | GTATAC | 1 cut(s) | 1149 |
| BssT1I | CCWWGG | 1 cut(s) | 1209 |
| Bst1107I | GTATAC | 1 cut(s) | 1149 |
| Bst2UI | CCWGG | 3 cut(s) | 578, 759, 1053 |
| Bst4CI | ACNGT | 5 cut(s) | 227, 487, 673, 678, 797 |
| Bst6I | CTCTTC | 1 cut(s) | 465 |
| BstBAI | YACGTR | 1 cut(s) | 1108 |
| BstC8I | GCNNGC | 8 cut(s) | 78, 219, 277, 432, 697, 804, 808, 1203 |
| BstDEI | CTNAG | 3 cut(s) | 1118, 1164, 1182 |
| BstF5I | GGATG | 3 cut(s) | 241, 322, 472 |
| BstFNI | CGCG | 1 cut(s) | 831 |
| BstHHI | GCGC | 1 cut(s) | 833 |
| BstMAI | GTCTC | 1 cut(s) | 1064 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 77, 902 |
| BstNI | CCWGG | 3 cut(s) | 578, 759, 1053 |
| BstNSI | RCATGY | 1 cut(s) | 221 |
| BstSCI | CCNGG | 5 cut(s) | 576, 588, 757, 909, 1051 |
| BstSLI | GKGCMC | 2 cut(s) | 574, 630 |
| BstUI | CGCG | 1 cut(s) | 831 |
| BstV1I | GCAGC | 5 cut(s) | 34, 905, 916, 1192, 1213 |
| BstV2I | GAAGAC | 1 cut(s) | 447 |
| BstX2I | RGATCY | 2 cut(s) | 110, 155 |
| BstYI | RGATCY | 2 cut(s) | 110, 155 |
| BstZ17I | GTATAC | 1 cut(s) | 1149 |
| BsuI | GTATCC | 1 cut(s) | 280 |
| BsuRI | GGCC | 1 cut(s) | 279 |
| BtsCI | GGATG | 3 cut(s) | 241, 322, 472 |
| BtsI | GCAGTG | 1 cut(s) | 744 |
| BtsIMutI | CAGTG | 3 cut(s) | 492, 744, 1076 |
| Cac8I | GCNNGC | 8 cut(s) | 78, 219, 277, 432, 697, 804, 808, 1203 |
| CciI | TCATGA | 1 cut(s) | 90 |
| CfoI | GCGC | 1 cut(s) | 833 |
| Cfr10I | RCCGGY | 1 cut(s) | 296 |
| CsiI | ACCWGGT | 1 cut(s) | 1051 |
| Csp6I | GTAC | 5 cut(s) | 674, 827, 836, 1105, 1113 |
| CspAI | ACCGGT | 1 cut(s) | 296 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1036, 1071 |
| CviAII | CATG | 4 cut(s) | 91, 218, 857, 1126 |
| CviQI | GTAC | 5 cut(s) | 674, 827, 836, 1105, 1113 |
| DdeI | CTNAG | 3 cut(s) | 1118, 1164, 1182 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1134 |
| Eam1104I | CTCTTC | 1 cut(s) | 465 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1134 |
| EarI | CTCTTC | 1 cut(s) | 465 |
| EciI | GGCGGA | 1 cut(s) | 714 |
| Eco130I | CCWWGG | 1 cut(s) | 1209 |
| Eco147I | AGGCCT | 1 cut(s) | 279 |
| Eco31I | GGTCTC | 1 cut(s) | 1064 |
| Eco32I | GATATC | 1 cut(s) | 262 |
| Eco57I | CTGAAG | 2 cut(s) | 302, 633 |
| Eco88I | CYCGRG | 2 cut(s) | 462, 1015 |
| EcoRI | GAATTC | 1 cut(s) | 545 |
| EcoRII | CCWGG | 3 cut(s) | 576, 757, 1051 |
| EcoRV | GATATC | 1 cut(s) | 262 |
| EcoT14I | CCWWGG | 1 cut(s) | 1209 |
| EcoT22I | ATGCAT | 1 cut(s) | 219 |
| ErhI | CCWWGG | 1 cut(s) | 1209 |
| FaeI | CATG | 4 cut(s) | 94, 221, 860, 1129 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 889, 921 |
| FaqI | GGGAC | 1 cut(s) | 22 |
| FatI | CATG | 4 cut(s) | 90, 217, 856, 1125 |
| FblI | GTMKAC | 2 cut(s) | 202, 1148 |
| Fnu4HI | GCNGC | 5 cut(s) | 48, 894, 930, 1206, 1227 |
| FokI | GGATG | 3 cut(s) | 248, 309, 479 |
| Fsp4HI | GCNGC | 5 cut(s) | 48, 894, 930, 1206, 1227 |
| FspBI | CTAG | 3 cut(s) | 77, 209, 839 |
| GlaI | GCGC | 1 cut(s) | 832 |
| GluI | GCNGC | 5 cut(s) | 48, 894, 930, 1206, 1227 |
| GsaI | CCCAGC | 1 cut(s) | 75 |
| HaeIII | GGCC | 1 cut(s) | 279 |
| HapII | CCGG | 3 cut(s) | 297, 589, 910 |
| HhaI | GCGC | 1 cut(s) | 833 |
| Hin1II | CATG | 4 cut(s) | 94, 221, 860, 1129 |
| Hin6I | GCGC | 1 cut(s) | 831 |
| HinP1I | GCGC | 1 cut(s) | 831 |
| HincII | GTYRAC | 2 cut(s) | 823, 865 |
| HindII | GTYRAC | 2 cut(s) | 823, 865 |
| HindIII | AAGCTT | 1 cut(s) | 428 |
| HinfI | GANTC | 2 cut(s) | 866, 1073 |
| HpaII | CCGG | 3 cut(s) | 297, 589, 910 |
| HphI | GGTGA | 5 cut(s) | 269, 311, 661, 775, 1016 |
| Hpy166II | GTNNAC | 8 cut(s) | 203, 572, 583, 628, 647, 823, 865, 1149 |
| Hpy188I | TCNGA | 3 cut(s) | 613, 992, 1194 |
| Hpy188III | TCNNGA | 9 cut(s) | 34, 85, 91, 209, 437, 464, 515, 538, 603 |
| Hpy8I | GTNNAC | 8 cut(s) | 203, 572, 583, 628, 647, 823, 865, 1149 |
| HpyAV | CCTTC | 5 cut(s) | 181, 277, 456, 460, 488 |
| HpyCH4III | ACNGT | 5 cut(s) | 227, 487, 673, 678, 797 |
| HpyCH4IV | ACGT | 3 cut(s) | 825, 1107, 1173 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 77, 902 |
| HpyF3I | CTNAG | 3 cut(s) | 1118, 1164, 1182 |
| HpySE526I | ACGT | 3 cut(s) | 825, 1107, 1173 |
| Hsp92II | CATG | 4 cut(s) | 94, 221, 860, 1129 |
| HspAI | GCGC | 1 cut(s) | 831 |
| LmnI | GCTCC | 2 cut(s) | 413, 902 |
| Lsp1109I | GCAGC | 5 cut(s) | 34, 905, 916, 1192, 1213 |
| LweI | GCATC | 3 cut(s) | 4, 520, 1151 |
| MabI | ACCWGGT | 1 cut(s) | 1051 |
| MaeI | CTAG | 3 cut(s) | 77, 209, 839 |
| MaeII | ACGT | 3 cut(s) | 825, 1107, 1173 |
| MaeIII | GTNAC | 3 cut(s) | 311, 667, 970 |
| MboII | GAAGA | 4 cut(s) | 452, 482, 992, 1229 |
| MfeI | CAATTG | 1 cut(s) | 887 |
| MflI | RGATCY | 2 cut(s) | 110, 155 |
| MhlI | GDGCHC | 3 cut(s) | 336, 574, 630 |
| MluCI | AATT | 5 cut(s) | 363, 506, 545, 656, 887 |
| MlyI | GAGTC | 1 cut(s) | 860 |
| Mph1103I | ATGCAT | 1 cut(s) | 219 |
| MroXI | GAANNNNTTC | 1 cut(s) | 753 |
| MseI | TTAA | 5 cut(s) | 167, 366, 426, 714, 1011 |
| MspI | CCGG | 3 cut(s) | 297, 589, 910 |
| MspR9I | CCNGG | 5 cut(s) | 578, 590, 759, 911, 1053 |
| MunI | CAATTG | 1 cut(s) | 887 |
| Mva1269I | GAATGC | 1 cut(s) | 80 |
| MvaI | CCWGG | 3 cut(s) | 578, 759, 1053 |
| MvnI | CGCG | 1 cut(s) | 831 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 77, 902 |
| NciI | CCSGG | 2 cut(s) | 590, 911 |
| NheI | GCTAGC | 1 cut(s) | 76 |
| NlaIII | CATG | 4 cut(s) | 94, 221, 860, 1129 |
| NlaIV | GGNNCC | 4 cut(s) | 63, 112, 157, 1083 |
| NmuCI | GTSAC | 2 cut(s) | 311, 667 |
| NsiI | ATGCAT | 1 cut(s) | 219 |
| NspI | RCATGY | 1 cut(s) | 221 |
| PaeI | GCATGC | 1 cut(s) | 221 |
| PaeR7I | CTCGAG | 1 cut(s) | 1015 |
| PagI | TCATGA | 1 cut(s) | 90 |
| PceI | AGGCCT | 1 cut(s) | 279 |
| PctI | GAATGC | 1 cut(s) | 80 |
| PdmI | GAANNNNTTC | 1 cut(s) | 753 |
| PfeI | GAWTC | 1 cut(s) | 1073 |
| Pfl23II | CGTACG | 2 cut(s) | 673, 826 |
| PflFI | GACNNNGTC | 1 cut(s) | 651 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 71 |
| PfoI | TCCNGGA | 1 cut(s) | 909 |
| PinAI | ACCGGT | 1 cut(s) | 296 |
| PkrI | GCNGC | 5 cut(s) | 49, 895, 931, 1207, 1228 |
| PleI | GAGTC | 1 cut(s) | 860 |
| PpsI | GAGTC | 1 cut(s) | 860 |
| Ppu21I | YACGTR | 1 cut(s) | 1108 |
| Psp6I | CCWGG | 3 cut(s) | 576, 757, 1051 |
| PspFI | CCCAGC | 1 cut(s) | 71 |
| PspGI | CCWGG | 3 cut(s) | 576, 757, 1051 |
| PspLI | CGTACG | 2 cut(s) | 673, 826 |
| PspN4I | GGNNCC | 4 cut(s) | 63, 112, 157, 1083 |
| PspXI | VCTCGAGB | 1 cut(s) | 1015 |
| PsuI | RGATCY | 2 cut(s) | 110, 155 |
| PsyI | GACNNNGTC | 1 cut(s) | 651 |
| RsaI | GTAC | 5 cut(s) | 675, 828, 837, 1106, 1114 |
| RsaNI | GTAC | 5 cut(s) | 674, 827, 836, 1105, 1113 |
| SaqAI | TTAA | 5 cut(s) | 167, 366, 426, 714, 1011 |
| SatI | GCNGC | 5 cut(s) | 48, 894, 930, 1206, 1227 |
| SchI | GAGTC | 1 cut(s) | 860 |
| ScrFI | CCNGG | 5 cut(s) | 578, 590, 759, 911, 1053 |
| SduI | GDGCHC | 3 cut(s) | 336, 574, 630 |
| SexAI | ACCWGGT | 1 cut(s) | 1051 |
| SfaNI | GCATC | 3 cut(s) | 4, 520, 1151 |
| Sfr274I | CTCGAG | 1 cut(s) | 1015 |
| SlaI | CTCGAG | 1 cut(s) | 1015 |
| SmlI | CTYRAG | 6 cut(s) | 435, 515, 536, 868, 1015, 1061 |
| SmoI | CTYRAG | 6 cut(s) | 435, 515, 536, 868, 1015, 1061 |
| SpeI | ACTAGT | 1 cut(s) | 838 |
| SphI | GCATGC | 1 cut(s) | 221 |
| Sse9I | AATT | 5 cut(s) | 363, 506, 545, 656, 887 |
| SseBI | AGGCCT | 1 cut(s) | 279 |
| SsiI | CCGC | 2 cut(s) | 699, 1091 |
| SspMI | CTAG | 3 cut(s) | 77, 209, 839 |
| StuI | AGGCCT | 1 cut(s) | 279 |
| StyD4I | CCNGG | 5 cut(s) | 576, 588, 757, 909, 1051 |
| StyI | CCWWGG | 1 cut(s) | 1209 |
| TaaI | ACNGT | 5 cut(s) | 227, 487, 673, 678, 797 |
| TaiI | ACGT | 3 cut(s) | 828, 1110, 1176 |
| TaqI | TCGA | 3 cut(s) | 336, 602, 1016 |
| TasI | AATT | 5 cut(s) | 363, 506, 545, 656, 887 |
| TatI | WGTACW | 1 cut(s) | 835 |
| TfiI | GAWTC | 1 cut(s) | 1073 |
| Tru1I | TTAA | 5 cut(s) | 167, 366, 426, 714, 1011 |
| Tru9I | TTAA | 5 cut(s) | 167, 366, 426, 714, 1011 |
| TscAI | CASTG | 3 cut(s) | 492, 744, 1083 |
| TseFI | GTSAC | 2 cut(s) | 311, 667 |
| TseI | GCWGC | 5 cut(s) | 47, 893, 929, 1205, 1226 |
| Tsp45I | GTSAC | 2 cut(s) | 311, 667 |
| TspDTI | ATGAA | 4 cut(s) | 17, 483, 782, 971 |
| TspRI | CASTG | 3 cut(s) | 492, 744, 1083 |
| Tth111I | GACNNNGTC | 1 cut(s) | 651 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 71 |
| VneI | GTGCAC | 2 cut(s) | 570, 626 |
| XapI | RAATTY | 1 cut(s) | 545 |
| XbaI | TCTAGA | 1 cut(s) | 208 |
| XceI | RCATGY | 1 cut(s) | 221 |
| XhoI | CTCGAG | 1 cut(s) | 1015 |
| XmiI | GTMKAC | 2 cut(s) | 202, 1148 |
| XmnI | GAANNNNTTC | 1 cut(s) | 753 |
| XspI | CTAG | 3 cut(s) | 77, 209, 839 |
| Zsp2I | ATGCAT | 1 cut(s) | 219 |