Rh7CG206700

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0010128
Physical Location & Seq
Forward (+)
18472745 .. 18476794
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG206700.1

Sequence Viewer

Length: 1716 bp
ATGGCTACAGAGTCAGTCAGAGTAGTCCACAGTTTCAGCTTCAATGGGTCTCAGACAAGGCCTACCATCCCTCTCTCCCCAGCTCGCTTTCTGGGTCTTCGTCCTCCTCGTTCTTCTTCTTCTTCTTCTTCTCTCACCTCGTCTTCACTTTCCCAGTTCTTTGGGAATCTGCGTCTCACCTCCAACTCCTCAAAGACCTCTCATTTGCGCCAACACAGCCGAAGAAACCTCTCTGTTTTCGCCATGGCCGCCGATGATGGAAAGCGTGCAGTACCATTGGTAGATTATCGGAATATTGGAATTATGGCTCACATAGATGCAGGGAAGACTACTACAACTGAACGGATTTTGTACTATACCGGAAGAAACTATAAAATTGGGGAGGTGCATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCAGCAACTACAACATTTTGGGACAAACACAGAATTAACATTATTGATACTCCTGGCCATGTGGACTTCACCCTCGAAGTGGAGCGAGCTCTCAGGGTACTTGATGGCGCAATATGCTTGTTTGACAGTGTTGCTGGGGTGGAACCTCAGTCTGAAACTGTGTGGAGGCAAGCTGATAAATATGGGGTGCCCAGGATTTGCTTTGTCAACAAAATGGATCGTCTTGGGGCAAACTTTTTCCGCACAAGGGACATGATAGTGACAAATTTGGGTGCCAAACCACTTGTACTTCAAATTCCAGTTGGTGCCGAAGATAATTTTAAGGGAGTTATTGATCTTGTGAAGATGAGAGCTATACTTTGGTCAGGAGAAGAATTGGGTGCAAAGTTTTCGTATGAGGATATTCCATCTGATCTTCAGGAGTTGGCTGACGAATACAGGTCACTGATGATAGAAACCATAGTTGAGTTGGATGATGAAGCTATGGAGGGCTATCTAGAAGGAGTTGAACCTGATGAGGCCACCATTAAGAAATTGATTAGGCAGGGAACCATCTCAGCTAGTTTTGTGCCAGTATTATGTGGCTCAGCTTTTAAAAATAAGGGGGTTCAACCATTGCTCGATGCTGTCGTGGATTATTTACCTTCACCGCTTGATGTGCCACCAATGCAGGGAACTGATGCAGATAACCCAGAAATCACGATTGAGAGGGCTGCAAGTGATGATGAACCATTTGCTGGGCTGGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATCTATGCAGGAAAGCTAACTGCTGGATCATACGTGCTGAATGCAAACAAGGGGAAAAAGGAGAGGATCGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTTAAGGTTGCTTTAGCTGGTGATATTGTTGCTCTTGCAGGTCTAAAAGATACTGTTACTGGTGAAACATTGAGTGACCCTGAGCATCCTATTGTGCTTGAACGTATGGAATTCCCTGATCCTGTGATTAAGGTGGCAATTGAACCCAAAACTAAAGCCGATGTTGATAAGATGGGAGCTGGTTTGGTTAAACTTGCTCAGGAAGACCCTTCGTTCCACTTCTCAAGGGATGAAGAGGTCAATCAGACAGTGATCGAAGGAATGGGTGAATTGCATCTTGAGATTATTGTTGATCGCCTCAAGAGGGAATTCAAGGTATATATCTGCTACTCCATGTTGCTGATTGTCAATTTCACGTAG

Protein Analysis

571

Amino Acids

63.22

Weight (kDa)

5.22

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 96 - 367 1.1e-67 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 395 - 477 8.8e-17 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 410 - 476 1.2e-16 Elongation factor Tu domain 2
EFG_III PF14492 490 - 559 2.6e-30 Elongation Factor G, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1385
AccB1I GGYRCC 3 cut(s) 640, 725, 758
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccI GTMKAC 1 cut(s) 1321
AciI CCGC 3 cut(s) 249, 694, 1103
AclWI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
AcoI YGGCCR 2 cut(s) 246, 508
AcsI RAATTY 4 cut(s) 718, 747, 1466, 1664
AcuI CTGAAG 1 cut(s) 854
AfaI GTAC 4 cut(s) 273, 353, 552, 741
AfiI CCNNNNNNNGG 6 cut(s) 532, 700, 1124, 1190, 1226, 1660
AgsI TTSAA 8 cut(s) 43, 746, 962, 1064, 1204, 1457, 1499, 1669
AjnI CCWGG 2 cut(s) 505, 644
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 54, 179
AlwI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
AoxI GGCC 4 cut(s) 59, 246, 508, 972
ApeKI GCWGC 2 cut(s) 455, 1166
ApoI RAATTY 4 cut(s) 718, 747, 1466, 1664
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
AspLEI GCGC 2 cut(s) 210, 563
AsuHPI GGTGA 7 cut(s) 127, 169, 514, 1092, 1388, 1430, 1634
BaeGI GKGCMC 1 cut(s) 645
BalI TGGCCA 1 cut(s) 510
BamHI GGATCC 1 cut(s) 1229
BanI GGYRCC 3 cut(s) 640, 725, 758
BanII GRGCYC 1 cut(s) 544
BbsI GAAGAC 4 cut(s) 89, 135, 332, 1566
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 467, 1153
BccI CCATC 7 cut(s) 74, 251, 409, 551, 868, 1013, 1522
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BciT130I CCWGG 2 cut(s) 507, 646
BcoDI GTCTC 2 cut(s) 54, 179
BfaI CTAG 3 cut(s) 950, 1014, 1328
BfmI CTRYAG 2 cut(s) 6, 453
BfuAI ACCTGC 1 cut(s) 1385
BisI GCNGC 3 cut(s) 249, 456, 1167
BlpI GCTNAGC 1 cut(s) 1039
BlsI GCNGC 3 cut(s) 250, 457, 1168
Bme1390I CCNGG 2 cut(s) 507, 646
BmiI GGNNCC 6 cut(s) 597, 642, 727, 760, 1003, 1231
BmrFI CCNGG 2 cut(s) 507, 646
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 5 cut(s) 307, 1066, 1123, 1450, 1639
BmuI ACTGGG 1 cut(s) 148
BpiI GAAGAC 4 cut(s) 89, 135, 332, 1566
Bpu10I CCTNAGC 2 cut(s) 1437, 1554
Bpu1102I GCTNAGC 1 cut(s) 1039
BpuEI CTTGAG 3 cut(s) 1564, 1640, 1655
BsaAI YACGTR 2 cut(s) 1282, 1713
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 243, 644
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 6 cut(s) 532, 700, 1124, 1190, 1226, 1660
Bse1I ACTGG 5 cut(s) 154, 416, 752, 1025, 1420
Bse3DI GCAATG 1 cut(s) 1067
BseBI CCWGG 2 cut(s) 507, 646
BseDI CCNNGG 2 cut(s) 243, 644
BseGI GGATG 6 cut(s) 66, 420, 931, 1360, 1441, 1591
BseLI CCNNNNNNNGG 6 cut(s) 532, 700, 1124, 1190, 1226, 1660
BseMI GCAATG 1 cut(s) 1067
BseMII CTCAG 7 cut(s) 65, 559, 614, 1023, 1053, 1428, 1568
BseNI ACTGG 5 cut(s) 154, 416, 752, 1025, 1420
BseRI GAGGAG 2 cut(s) 96, 178
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 2 cut(s) 467, 1153
BseYI CCCAGC 3 cut(s) 79, 587, 1190
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 4 cut(s) 61, 248, 510, 974
BshNI GGYRCC 3 cut(s) 640, 725, 758
BsiHKAI GWGCWC 1 cut(s) 544
BsiSI CCGG 1 cut(s) 360
BslFI GGGAC 2 cut(s) 488, 716
BslI CCNNNNNNNGG 6 cut(s) 532, 700, 1124, 1190, 1226, 1660
BsmAI GTCTC 2 cut(s) 54, 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 488, 716
BsmI GAATGC 2 cut(s) 1199, 1294
BsnI GGCC 4 cut(s) 61, 248, 510, 974
Bso31I GGTCTC 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 544, 645
Bsp1720I GCTNAGC 1 cut(s) 1039
Bsp19I CCATGG 1 cut(s) 243
BspACI CCGC 3 cut(s) 249, 694, 1103
BspANI GGCC 4 cut(s) 61, 248, 510, 974
BspCNI CTCAG 7 cut(s) 64, 558, 613, 1022, 1052, 1429, 1567
BspHI TCATGA 1 cut(s) 1209
BspLI GGNNCC 6 cut(s) 597, 642, 727, 760, 1003, 1231
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 1385
BspPI GGATC 7 cut(s) 678, 1211, 1224, 1237, 1282, 1322, 1469
BspT107I GGYRCC 3 cut(s) 640, 725, 758
BspTNI GGTCTC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 1067
BsrI ACTGG 5 cut(s) 154, 416, 752, 1025, 1420
BssECI CCNNGG 2 cut(s) 243, 644
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 2 cut(s) 507, 646
Bst4CI ACNGT 6 cut(s) 32, 581, 613, 1346, 1411, 1606
Bst6I CTCTTC 1 cut(s) 1584
BstBAI YACGTR 2 cut(s) 1282, 1713
BstC8I GCNNGC 6 cut(s) 85, 267, 540, 624, 1197, 1338
BstDEI CTNAG 7 cut(s) 51, 545, 600, 1009, 1039, 1437, 1554
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 6 cut(s) 66, 420, 931, 1360, 1441, 1591
BstHHI GCGC 2 cut(s) 210, 563
BstMAI GTCTC 2 cut(s) 54, 179
BstMWI GCNNNNNNNGC 6 cut(s) 216, 248, 567, 1111, 1120, 1196
BstNI CCWGG 2 cut(s) 507, 646
BstNSI RCATGY 1 cut(s) 1340
BstSCI CCNGG 2 cut(s) 505, 644
BstSFI CTRYAG 2 cut(s) 6, 453
BstSLI GKGCMC 1 cut(s) 645
BstV1I GCAGC 2 cut(s) 467, 1153
BstV2I GAAGAC 4 cut(s) 89, 135, 332, 1566
BstX2I RGATCY 1 cut(s) 1229
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 1229
BsuRI GGCC 4 cut(s) 61, 248, 510, 974
BtgI CCRYGG 1 cut(s) 243
BtsCI GGATG 6 cut(s) 66, 420, 931, 1360, 1441, 1591
BtsIMutI CAGTG 3 cut(s) 586, 896, 1611
BveI ACCTGC 1 cut(s) 1385
Cac8I GCNNGC 6 cut(s) 85, 267, 540, 624, 1197, 1338
CciI TCATGA 1 cut(s) 1209
CfoI GCGC 2 cut(s) 210, 563
CseI GACGC 1 cut(s) 161
Csp6I GTAC 4 cut(s) 272, 352, 551, 740
CviAII CATG 7 cut(s) 244, 389, 512, 706, 1210, 1337, 1690
CviQI GTAC 4 cut(s) 272, 352, 551, 740
DdeI CTNAG 7 cut(s) 51, 545, 600, 1009, 1039, 1437, 1554
DraI TTTAAA 1 cut(s) 1048
EaeI YGGCCR 2 cut(s) 246, 508
Eam1104I CTCTTC 1 cut(s) 1584
EarI CTCTTC 1 cut(s) 1584
Ecl136II GAGCTC 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 243
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 542
EcoRI GAATTC 2 cut(s) 1466, 1664
EcoRII CCWGG 2 cut(s) 505, 644
EcoT14I CCWWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 1338
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 7 cut(s) 247, 392, 515, 709, 1213, 1340, 1693
FaqI GGGAC 2 cut(s) 488, 716
FatI CATG 7 cut(s) 243, 388, 511, 705, 1209, 1336, 1689
FblI GTMKAC 1 cut(s) 1321
Fnu4HI GCNGC 3 cut(s) 249, 456, 1167
FokI GGATG 6 cut(s) 53, 427, 938, 1367, 1428, 1598
FriOI GRGCYC 1 cut(s) 544
Fsp4HI GCNGC 3 cut(s) 249, 456, 1167
FspBI CTAG 3 cut(s) 950, 1014, 1328
GlaI GCGC 2 cut(s) 209, 562
GluI GCNGC 3 cut(s) 249, 456, 1167
GsaI CCCAGC 3 cut(s) 83, 591, 1194
HaeIII GGCC 4 cut(s) 61, 248, 510, 974
HapII CCGG 1 cut(s) 360
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 210, 563
Hin1II CATG 7 cut(s) 247, 392, 515, 709, 1213, 1340, 1693
Hin6I GCGC 2 cut(s) 208, 561
HinP1I GCGC 2 cut(s) 208, 561
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 3 cut(s) 11, 166, 1248
HpaII CCGG 1 cut(s) 360
HphI GGTGA 7 cut(s) 127, 169, 514, 1092, 1388, 1430, 1634
Hpy166II GTNNAC 4 cut(s) 28, 517, 661, 1322
Hpy188I TCNGA 6 cut(s) 20, 54, 291, 607, 865, 1602
Hpy8I GTNNAC 4 cut(s) 28, 517, 661, 1322
HpyAV CCTTC 5 cut(s) 386, 947, 1107, 1575, 1607
HpyCH4III ACNGT 6 cut(s) 32, 581, 613, 1346, 1411, 1606
HpyCH4IV ACGT 3 cut(s) 1281, 1459, 1712
HpyF10VI GCNNNNNNNGC 6 cut(s) 216, 248, 567, 1111, 1120, 1196
HpyF3I CTNAG 7 cut(s) 51, 545, 600, 1009, 1039, 1437, 1554
HpySE526I ACGT 3 cut(s) 1281, 1459, 1712
Hsp92II CATG 7 cut(s) 247, 392, 515, 709, 1213, 1340, 1693
HspAI GCGC 2 cut(s) 208, 561
LmnI GCTCC 3 cut(s) 418, 535, 1532
Lsp1109I GCAGC 2 cut(s) 467, 1153
LweI GCATC 5 cut(s) 307, 1066, 1123, 1450, 1639
MaeI CTAG 3 cut(s) 950, 1014, 1328
MaeII ACGT 3 cut(s) 1281, 1459, 1712
MaeIII GTNAC 4 cut(s) 712, 894, 1411, 1430
MfeI CAATTG 1 cut(s) 1494
MflI RGATCY 1 cut(s) 1229
MhlI GDGCHC 2 cut(s) 544, 645
MlsI TGGCCA 1 cut(s) 510
MluNI TGGCCA 1 cut(s) 510
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 207, 903
Mox20I TGGCCA 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 1338
MscI TGGCCA 1 cut(s) 510
MseI TTAA 7 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545
MslI CAYNNNNRTG 2 cut(s) 315, 710
Msp20I TGGCCA 1 cut(s) 510
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 2 cut(s) 507, 646
MunI CAATTG 1 cut(s) 1494
Mva1269I GAATGC 2 cut(s) 1199, 1294
MvaI CCWGG 2 cut(s) 507, 646
MwoI GCNNNNNNNGC 6 cut(s) 216, 248, 567, 1111, 1120, 1196
NcoI CCATGG 1 cut(s) 243
NlaIII CATG 7 cut(s) 247, 392, 515, 709, 1213, 1340, 1693
NlaIV GGNNCC 6 cut(s) 597, 642, 727, 760, 1003, 1231
NmuCI GTSAC 3 cut(s) 712, 894, 1430
NsiI ATGCAT 1 cut(s) 1338
NspI RCATGY 1 cut(s) 1340
PaeI GCATGC 1 cut(s) 1340
PagI TCATGA 1 cut(s) 1209
PceI AGGCCT 1 cut(s) 61
PcsI WCGNNNNNNNCGW 2 cut(s) 106, 1080
PctI GAATGC 2 cut(s) 1199, 1294
PfeI GAWTC 2 cut(s) 166, 1248
PflMI CCANNNNNTGG 1 cut(s) 1190
PkrI GCNGC 3 cut(s) 250, 457, 1168
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 2 cut(s) 1282, 1713
Psp124BI GAGCTC 1 cut(s) 544
Psp6I CCWGG 2 cut(s) 505, 644
PspFI CCCAGC 3 cut(s) 79, 587, 1190
PspGI CCWGG 2 cut(s) 505, 644
PspN4I GGNNCC 6 cut(s) 597, 642, 727, 760, 1003, 1231
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 1229
RsaI GTAC 4 cut(s) 273, 353, 552, 741
RsaNI GTAC 4 cut(s) 272, 352, 551, 740
RseI CAYNNNNRTG 2 cut(s) 315, 710
SacI GAGCTC 1 cut(s) 544
SaqAI TTAA 7 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545
SatI GCNGC 3 cut(s) 249, 456, 1167
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 507, 646
SduI GDGCHC 2 cut(s) 544, 645
SfaNI GCATC 5 cut(s) 307, 1066, 1123, 1450, 1639
SfcI CTRYAG 2 cut(s) 6, 453
SmiMI CAYNNNNRTG 2 cut(s) 315, 710
SmlI CTYRAG 3 cut(s) 1579, 1634, 1655
SmoI CTYRAG 3 cut(s) 1579, 1634, 1655
SphI GCATGC 1 cut(s) 1340
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 3 cut(s) 249, 694, 1103
SspI AATATT 1 cut(s) 295
SspMI CTAG 3 cut(s) 950, 1014, 1328
SstI GAGCTC 1 cut(s) 544
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 505, 644
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 6 cut(s) 32, 581, 613, 1346, 1411, 1606
TaiI ACGT 3 cut(s) 1284, 1462, 1715
TaqI TCGA 3 cut(s) 528, 1074, 1611
TatI WGTACW 2 cut(s) 351, 739
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 2 cut(s) 166, 1248
Tru1I TTAA 7 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545
Tru9I TTAA 7 cut(s) 489, 774, 981, 1047, 1359, 1485, 1545
TscAI CASTG 3 cut(s) 586, 903, 1611
TseFI GTSAC 3 cut(s) 712, 894, 1430
TseI GCWGC 2 cut(s) 455, 1166
Tsp45I GTSAC 3 cut(s) 712, 894, 1430
TspDTI ATGAA 4 cut(s) 405, 945, 1194, 1602
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 3 cut(s) 586, 903, 1611
Van91I CCANNNNNTGG 1 cut(s) 1190
XapI RAATTY 4 cut(s) 718, 747, 1466, 1664
XbaI TCTAGA 2 cut(s) 949, 1327
XceI RCATGY 1 cut(s) 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmiI GTMKAC 1 cut(s) 1321
XspI CTAG 3 cut(s) 950, 1014, 1328
Zsp2I ATGCAT 1 cut(s) 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.